Results

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Our Results

The results of the analysis of Chelonoidis abingdonii selenoproteome are shown in the following table. The abbreviations used in the third column are defined in this list:

  • NA: not applicable, there is a gap where should be a Sec.
  • ND: no data (the database does not specify the position where Cys or Sec is supposed to be in the query).
  • Sec: Sec can be found both in human and tortoise proteins.
  • Sec lost: Sec can be found in the human's protein but has been lost in the tortoise's protein.
  • Sec gain: Sec cannot be found in the human's protein but has been gained in the tortoise's protein.
  • /: selenium machinery protein and has no selenocysteine.
  • Cys-X: cystein containing homolog in human, X indicates the residue in the tortoise's protein.
  • X-Y: other aminoacid containing homolog in human, X is the residue in the human's protein, Y is the residue in the tortoise's protein.

Table of results

Protein Species Residue Scaffold Gene Location Tblastn Gene in .gff Protein prediction Genewise prediction T-coffee Seblastian SECIS candidate SECIS image
Iodothyronine deiodinases
DI1 H. sapiens Sec PKMU01008277.1 1055125-1063829
DI2 H. sapiens Sec PKMU01005068.1 5506389-5517365
DI3 H. sapiens Sec PKMU01001135.1 242560-243354
Glutathione peroxidases
GPx1 H. sapiens Sec PKMU01007833.1 511801-513930
GPx2 H. sapiens Sec PKMU01001079.1 945493-947522
GPx3 H. sapiens Sec PKMU01000857.1 2069251-2077792
GPx4 H. sapiens Sec PKMU01000001.1 5440036-5442834
GPx7 H. sapiens Cys-Cys PKMU01008789.1 526415-542110
GPx8 H. sapiens Cys-Cys PKMU01009122.1 774516-779774
Methionine-R-Sulfoxide Reductase A
MsrA H. sapiens Cys-Cys PKMU01001278.1 963309-1272944
15 kDa selenoprotein + Selenoprotein M
Sel15 H. sapiens Sec PKMU01001331.1 129427-163444
SelM H. sapiens Sel PKMU01008734.1 2162444-2176610
Selenoprotein H
SelH H. sapiens Sec PKMU01001079.1 270158-271404
Selenoprotein I
SelI H. sapiens Sec PKMU01004179.1 933317-989453
Selenoprotein K + Selenoprotein S
SelK H. sapiens NA PKMU01003079.1 139808-143690
SelS H. sapiens Sec PKMU01007956.1 982332-988379
Selenoprotein N
SelN H. sapiens Sec PKMU01005290.1 4718252-4730686
Selenoprotein O
SelO H. sapiens NA PKMU01009289.1 1697628-1711296
Selenoprotein P
SelP A. carolinensis Sec PKMU01005800.1 532753-536049
Selenoprotein Pb
SelPb G. gallus Sec PKMU01005800.1 532717-536082
Methionine-R-sulfoxide reductase 1 (Selenoprotein R)
SelR1 H. sapiens Sec PKMU01006623.1 741771-743931
SelR2 A. carolinensis 5 Sec PKMU01001068.1 2087949-2101199
SelR3 H. sapiens Cys-Cys PKMU01002212.1 5686-101324
Selenoprotein T
SelT H. sapiens Sec PKMU01006301.1 1265830-1280060
Thioredoxin reductases
TR1 H. sapiens Sec PKMU01001697.1 81770-110367
TR2 H. sapiens Sec and Sec gain PKMU01010066.1 69617-117992
TR3 H. sapiens Sec PKMU01001735.1 7625889-7643891
Selenoprotein U
SelU1 H. sapiens Cys-Cys PKMU01001923.1 404239-417868
SelU2 H. sapiens Cys-Cys PKMU01002121.1 38721-43354
SelU3 H. sapiens Cys-Cys PKMU01007067.1 4850194-4862512
Selenoprotein W
SelW1 H. sapiens NA PKMU01003401.1 1603706-1606321
SelW2 H. sapiens Cys-Cys PKMU01002623.1 172868-174789
Selenoprotein machinery
eEFsec H. sapiens / PKMU01005278.1 625504-802532
SBP2 A. carolinensis / PKMU01000807.1 1070817-1100301
PSTK A. carolinensis / PKMU01003645.1 1863877-1870699
SECp43 H. sapiens Sec gain PKMU01005290.1 1867920-1881788
SecS A. carolinensis / PKMU01006445.1 939558-992103
SPS1 H. sapiens / PKMU01001170.1 242377-263996
SPS2 H. sapiens / PKMU01001233.1 112584-118501

Description of selenoproteins

Iodothyronine deiodinases

DI1

The protein DI1 is located in the scaffold PKMU01008277.1 between the position 1055125 and the position 1063829 in the reverse strand. This gene contains 4 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:1063520-1063829
            Exon 2:1061644-1061787
	    Exon 3:1056148-1056347
            Exon 4:1055125-1055184

Seblastian was able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii (DI1). Regarding the SECIS, one grade A SECIS was found between the locations 1053908-1053976. This secis is located in the same strand of our gene, and after it.

DI2

The protein DI2 is located in the scaffold PKMU01005068.1 between the position 5517365 and 5506389 in the reverse strand. This gene contains 2 exons. The structure of the gene, analyzed with the Exonerate file, is described below: 

            Exon 1:5517144-5517365
            Exon 2:5506389-5506958

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 5501787-5501861. This secis is located in the same strand of our gene, and after it.

DI3

The protein DI2 is located in the scaffold PKMU01005068.1 between the position 5517365 and 5506389 in the reverse strand. This gene contains 2 exons. The structure of the gene, analyzed with the Exonerate file, is described below: 

            Exon 1:242560-243354

Seblastian was able to predict a known selenoprotein (DI3) using the sequence from Chelonoidis abingdonii. Regarding the SECIs, one grade A SECIS was found between the locations 242096-242179. This secis is located in the same strand of our gene, and after it.

Glutathione peroxidases

GPx1

The protein GPx1 is located in the scaffold PKMU01007833.1. between the position 511801 and 513930 in the forward strand. This gene contains 2 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:511801-512028
            Exon 2:513583-513930

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIs, one grade A SECIS was found between the locations  514100-514171. This secis is located in the same strand of our gene, and after it.

GPx2

The protein GPx2 is located in the scaffold PKMU01001079.1. between the position 945493 and 947522 in the forward strand. This gene contains 2 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:945493-945714
            Exon 2:947178-947522

Seblastian was able to predict a known selenoprotein (GPx2) using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations  947635-947700. This secis is located in the same strand of our gene, and after it.

GPx3

The protein GPx3 is located in the scaffold PKMU01000857.1 between the position 2069251 and 2077792 in the forward strand. This gene contains 5 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:2077706-2077792
            Exon 2:2071532-2071685
	    Exon 3:2070740-2070857
            Exon 4:2069570-2069669
	    Exon 5:2069251-2069445

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. It was also not capable of finding a SECIS.

GPx4

The protein GPx4 is located in the scaffold PKMU01000001.1 between the position 5440036 and 5442834 in the forward strand. This gene contains 5 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:5440036-5440098
            Exon 2:5440765-5440859
	    Exon 3:5441413-5441557
            Exon 4:5442285-5442436
	    Exon 5:5442810-5442834

Seblastian was able to predict a selenoprotein (GPx4) using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 5444262-5444334. This SECIS is located in the same strand of our gene, and after it.

GPx7

The protein GPx7 is located in the scaffold PKMU01008789.1 between the position 526415 and 542110 in the reverse strand. This gene contains 3 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:541973-542110
            Exon 2:529861-530122
	    Exon 3:526415-526575

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade B SECIS was found between the locations 481570-481653. This SECIS is located in the same strand of our gene, and after it.

GPx8

The protein GPx8 is located in the scaffold PKMU01009122.1 between the position 774516 and 779774 in the reverse strand. This gene contains 3 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:779571-779774
            Exon 2:778654-778855
	    Exon 3:774516-774676

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIS has a grade B and it is found in the positions 743796-743887. This SECIS is located in the same strand of our gene, and after it.

MsrA

The protein MsrA is located in the scaffold PKMU01001278.1 between the position 963309 and the position 1272944 in the reverse strand. This gene contains 5 exons. The structure of the gene, analyzed with the Exonerate file, is described below: 

            Exon 1:1272869-1272944
            Exon 2:1205329-1205448
	    Exon 3:1160669-1160773
	    Exon 4:1107815-1107921
            Exon 5:963309-963470

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade B SECIS was found between the locations 1311451-1311522. This SECIS is located in the same strand of our gene, and before it.

Sel15 + SelM

Sel15

The protein Sel15 is located in the scaffold PKMU01001331.1 between the position 129427 and the position 163444 in the forward strand. This gene contains 2 exons. The structure of the gene, analyzed with the Exonerate file, is described below: 

            Exon 1:129427-129594
            Exon 2:163382-163444

Seblastian was able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 176321-176391. This SECIS is located in the same strand of our gene, and after it.

SelM

The protein SelM is located in the scaffold PKMU01008734.1 between the position 2162444 and the position 2176610 in the forward strand. This gene contains 5 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:2162444-2162548
            Exon 2:2164587-2164622
	    Exon 3:2164931-2164965
	    Exon 4:2165693-2165771
            Exon 5:2176485-2176610

Seblastian was able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 2176985-2177053. This SECIS is located in the same strand of our gene, and after it.

SelH

The protein SelH is located in the scaffold PKMU01001079.1 between the position 270158 and the position 271404 in the forward strand. This gene contains 3 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:270158-270261
            Exon 2:270651-270814
	    Exon 3:271310-271404

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade B SECIS was found between the locations 261636-261716. This SECIS is located in the same strand of our gene, and before it.

SelI

The protein SelI is located in the scaffold PKMU01004179.1 between the position 933317 and the position 989453 in thereverse strand. This gene contains 10 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:989397-989453
            Exon 2:952272-952340
	    Exon 3:951822-951930
	    Exon 4:944147-977221
            Exon 5:943331-943593
	    Exon 6:941781-941889
	    Exon 7:940072-940120
            Exon 8:938323-938503
	    Exon 9:936516-936698
	    Exon 10:933317-933412

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 931948-932025. This SECIS is located in the same strand of our gene, and after it.

SelK + SelS

SelK

The protein SelK is located in the scaffold PKMU01003079.1 between the position 139808 and the position 143690 in the reverse strand. This gene contains 4 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:143672-143690
            Exon 2:141788-141878
	    Exon 3:140726-140806
	    Exon 4:139808-139886

Seblastian was able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 138734-138817. This SECIS is located in the same strand of our gene, and after it.

SelS

The protein SelS is located in the scaffold PKMU01007956.1 between the position 982332 and 988379 in the reverse strand. This gene contains 5 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:988238-988379
            Exon 2:986428-986534
	    Exon 3:984742-984831
	    Exon 4:984553-984628
	    Exon 5:982332-982417

Seblastian was able to predict a known selenoprotein (SelS) using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 981336-981416. This SECIS is located in the same strand of our gene, and after it.

SelN

The protein SelN is located in the scaffold PKMU01005290.1 between the position 4718252 and 4730686 in the reverse strand. This gene contains 11 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:4730555-4730686
            Exon 2:4729658-4729765
	    Exon 3:4728762-4728974
	    Exon 4:4728230-4728354
            Exon 5:4726797-4726934
	    Exon 6:4724987-4725068
	    Exon 7:4723718-4723906
            Exon 8:4722164-4722269
	    Exon 9:4721030-4721142
	    Exon 10:4720209-4720310
            Exon 11:4718252-4718416

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 4713150-4713220. This SECIS is located in the same strand of our gene, and after it.

SelO

The protein SelO is located in the scaffold PKMU01009289.1 between the position 1697628 and 1711296 in the forward strand. This gene contains 7 exons. The structure of the gene, analyzed with the Exonerate file, is described below: 

            Exon 1:1697628-1697833 
            Exon 2:1698873-1699053
	    Exon 3:1705233-1705363
	    Exon 4:1705801-1706081
            Exon 5:1706287-1706437
	    Exon 6:1708291-1708476
	    Exon 7:1711134-1711296

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii.  It was also not capable of finding a SECIS.

SelP

The protein SelP is located in the scaffold PKMU01005800.1 between the position 532753 and the position 536049 in the reverse strand. This gene contains 3 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:535874-536049
            Exon 2:535330-535542
	    Exon 3:532753-532864

Seblastian wasn’t able to predict either a selenoprotein nor a SECIS using the sequence from Chelonoidis abingdonii.

SelPb

The protein SelPb is located in the scaffold PKMU01005800.1 between the position 532717 and 536082 in the reverse strand. This gene contains 3 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:535874-536082
            Exon 2:535330-535542
	    Exon 3:532717-532864

Seblastian was not able to predict a selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 747203-747275. This secis is located in the same strand of our gene, and after it.

Methionine-R-sulfoxide reductase 1 (Selenoprotein R)

SelR1

The protein SelR1 is located in the scaffold PKMU01006623.1 between the position 741771 and 743931 in the forward strand. This gene contains 3 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:741771-741825
            Exon 2:743497-743639
	    Exon 3:743812-743931

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIS has a grade B and it is found in the positions 743796-743887. This SECIS is located in the same strand of our gene, and after it.

SelR2

The protein SelR2 is located in the scaffold PKMU01001068.1 between the position 2087949 and 2101199 in the forward strand. This gene contains 4 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:2087949-2088044
            Exon 2:2089032-2089108
	    Exon 3:2098989-2099137
	    Exon 4:2101104-2101199

Seblastian was not able to predict a selenoprotein using the sequence from Chelonoidis abingdonii. It was also not capable of finding a valid SECIS.

SelR3

The protein SelR3 is located in the scaffold PKMU01002212.1 between the position 5686 and 101324 in the forward strand. This gene contains 5 exons. The structure of the gene, analyzed with the Exonerate file, is described below: 

            Exon 1:5686-5798
            Exon 2:7513-7590
	    Exon 3:31343-31371 
	    Exon 2:87357-87454
	    Exon 3:101163-101324

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii.  It was also not capable of finding a SECIS.

SelT

The protein SelT is located in the scaffold  PKMU01006301.1 between the position 1265830 and 1280060 in the forward strand. This gene contains 5 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:1265830-1265960
            Exon 2:1272714-1272824
	    Exon 3:1274046-1274172
	    Exon 4:1276887-1276974
	    Exon 5:1279939-1280060

Seblastian was not able to predict a selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 1284080-1284155. This SECIS is located in the same strand of our gene, and after it.

Thioredoxin reductases

TR1

The protein TR1 is located in the scaffold PKMU01001697.1 between the position 81770 and the position 110367 in the forward strand. This gene contains 13 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:81770-81853
            Exon 2:83036-83108
	    Exon 3:83228-83347
	    Exon 4:84107-84249
	    Exon 5:84375-84490
	    Exon 6:86650-86875
	    Exon 7:90806-90898
	    Exon 8:93560-93636
	    Exon 9:96589-96745
	    Exon 10:98776-98883
	    Exon 11:101392-101487
	    Exon 12:104050-104184
	    Exon 13:110302-110367

Seblastian was able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIS has a grade A and it is found in the positions 110620-110696. This SECIS is located in the forward strand, in the 3’UTR region.

TR2

The protein TR2 is located in the scaffold PKMU01010066.1 between the position 69617 and the position 117992 in the reverse strand. This gene contains 15 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:117926-117992
            Exon 2:117309-117365
	    Exon 3:116575-116719
	    Exon 4:116172-116246
	    Exon 5:114430-114508
	    Exon 6:113415-113477
	    Exon 7:110122-110212
	    Exon 8:98614-98705
	    Exon 9:95813-95987
	    Exon 10:82834-82970
	    Exon 11:76810-76905
	    Exon 12:76456-76548
	    Exon 13:74476-74547
	    Exon 14:72488-72585
	    Exon 15:69617-69743

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIS has a grade A and it is found in the positions 65624-65701. This SECIS is located in the reverse strand, in the 3’UTR region.

TR3

The protein TR3 is located in the scaffold PKMU01001735.1 between the position 7625889 and the position 7643891 in the reverse strand. This gene contains 15 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:7645395-7645458
            Exon 2:7643782-7643891
	    Exon 3:7642249-7642365
	    Exon 4:7641571-7641643
	    Exon 5:7640172-7640291
	    Exon 6:7639507-7639649
	    Exon 7:7637563-7637678
	    Exon 8:7637223-7637448
	    Exon 9:7636864-7636956
	    Exon 10:7636260-7636336
	    Exon 11:7634744-7634900
	    Exon 12:7634370-7634477
	    Exon 13:7628505-7628600
	    Exon 14:7626314-7626448
	    Exon 15:7625889-7625954

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIs has a grade A and it is found in the positions 7625577-7625661. This secis is located in the reverse strand, in the 3’UTR region.

Selenoprotein U

SelU1

The protein SelU1 is located in the scaffold PKMU01001923.1 between the position 404239 and 417868 in the reverse strand. This gene contains 5 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:417691-417868
            Exon 2:415661-415752
	    Exon 3:407517-407657
	    Exon 4:406231-406395
	    Exon 5:404239-404328

Seblastian was not able to predict a selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, one grade A SECIS was found between the locations 403935-404006. This SECIS is located in the same strand of our gene, and after it.

SelU2

The protein SelU2 is located in the scaffold PKMU01002121.1 between the position 38721 and 4335 in the forward strand. This gene contains 6 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:38721-38798
            Exon 2:39308-39376
	    Exon 3:40655-40708
	    Exon 4:41119-41224
	    Exon 5:42446-42577
	    Exon 6:43230-43354

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii.  It was also not capable of finding a SECIS.

SelU3

The protein SelU3 is located in the scaffold PKMU01007067.1 between the position 4850194 and the position 4862512 in the reverse strand. This gene contains 6 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:4862450-4862512
            Exon 2:4860543-4860747
	    Exon 3:4858582-4858633
	    Exon 4:4857715-4857778
	    Exon 5:4857025-4857100
	    Exon 6:4850194-4850312

Seblastian wasn’t able to predict either a selenoprotein nor a SECIS using the sequence from Chelonoidis abingdonii.

Selenoprotein W

SelW1

The protein SelW1 is located in the scaffold PKMU01003401.1 between the position 1603706 and the position 1606321 in the reverse strand. This gene contains 3 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:1606268-1606321
            Exon 2:1605253-1605327
	    Exon 3:1603706-1603783

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIS has a grade A and it is found in the positions 1616552-1616462. This SECIS is located in the reverse strand, in the 3’UTR region.

SelW2

The protein SelW2 is located in the scaffold PKMU01002623.1 between the position 172868 and the position 174789 in the forward strand. This gene contains 3 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:172868-172964
            Exon 2:174039-174115
	    Exon 3:174709-174789

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIS has a grade B and it is found in the positions 175706-175783. This SECIS is located in the forward strand, in the 3’UTR region.

Selenoprotein machinery

eEFSec

The protein eEFSec is located in the scaffold PKMU01005278.1. between the position  625504 and 802532 in the forward strand. This gene contains 7 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:625504-625765
            Exon 2:687911-688118
	    Exon 3:693693-693759
	    Exon 4:697649-697813
	    Exon 5:733942-734583
	    Exon 6:753222-753378
	    Exon 7:802351-802532

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIs, one grade B SECIS was found between the locations 727708-727760. This SECIS is located in the other DNA strand and inside the predicted gene. That’s why we don’t have taken it into account in the figure and in the table of results.

SBP2

The protein SBP2 is located in the scaffold PKMU01000807.1 between the position 1070817 and the position 1100301 in the forward strand. This gene contains 16 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:1070817-1071412
            Exon 2:1072609-1072828
	    Exon 3:1077634-1077788
	    Exon 4:1078484-1078704
	    Exon 5:1079336-1079498
	    Exon 6:1081170-1081302
	    Exon 7:1082223-1082392
	    Exon 8:1083522-1083663
	    Exon 9:1083830-1083997
	    Exon 10:1085131-1085214
	    Exon 11:1085721-1085852
	    Exon 12:1092181-1092296
	    Exon 13:1093316-1093552
	    Exon 14:1096701-1096842
	    Exon 15:1097979-1098303
	    Exon 16:1100120-1100301

Seblastian wasn’t able to predict either a selenoprotein nor a SECIS using the sequence from Chelonoidis abingdonii.

PSTK

The protein PSTK is located in the scaffold PKMU01003645.1 between the position 1863877 and the position 1870699 in the reverse strand. This gene contains 6 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:1870514-1870699
            Exon 2:1868464-1868755
	    Exon 3:1867278-1867506
	    Exon 4:1864917-1864992
	    Exon 5:1864315-1864408
	    Exon 6:1863877-1864058

Seblastian wasn’t able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIS, none of them was found.

SECp43

The protein SECp43 is located in the scaffold PKMU01005290.1 between the position 1867920 and the position 1881788 in the forward strand. This gene contains 8 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:1867920-1868017
            Exon 2:1869156-1869255
	    Exon 3:1870426-1870478
	    Exon 4:1872058-1872189
	    Exon 5:1874294-1874413
	    Exon 6:1876445-1876607
	    Exon 7:1877174-1877207
	    Exon 8:1881655-1881788

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIs, one grade B SECIS was found between the locations 1930863-1930952. This SECIS is not located in the same strand of our gene.

SecS

The protein SecS is located in the scaffold PKMU01006445.1 between the position 939558 and the position 992103 in the forward strand. This gene contains 11 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:939558-939671
            Exon 2:950962-951116
	    Exon 3:953702-953820
	    Exon 4:953912-954070
	    Exon 5:956431-956584
	    Exon 6:960817-960919
	    Exon 7:967511-967640
	    Exon 8:967749-967840
	    Exon 9:988589-988682
            Exon 10:989345-989435
	    Exon 11:991920-992103

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. Regarding the SECIs, one grade B SECIS was found between the locations 972401-972474. This SECIS is located inside the gene.

SPS1

The protein SPS1 is located in the scaffold PKMU01001170.1 between the position 242377 and the position 263996 in the forward strand. This gene contains 8 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:242377-242569
            Exon 2:245419-245522
	    Exon 3:251707-251814
	    Exon 4:252834-252988
	    Exon 5:253914-254004
	    Exon 6:256919-257018
	    Exon 7:259573-259785
	    Exon 8:263785-263996

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIs has a grade B and it is found in the positions 213852-213924. This SECIS is located in the forward strand, in the 5’UTR region.

SPS2

The protein SPS2 is located in the scaffold PKMU01001233.1 between the position 112584 and the position 118501 in the reverse strand. This gene contains 7 exons. The structure of the gene, analyzed with the Exonerate file, is described below:

            Exon 1:118385-118501
            Exon 2:117840-117947
	    Exon 3:114996-115150
	    Exon 4:114349-114439
	    Exon 5:113994-114093
	    Exon 6:113406-113618
	    Exon 7:112584-112810

Seblastian was not able to predict a known selenoprotein using the sequence from Chelonoidis abingdonii. The accepted SECIs has a grade A and it is found in the positions 112034-111960. This SECIS is located in the reverse strand, in the 3’UTR region.

Pau Forte, Víctor Herrera, Xavi Marimon, Ana Yagüe.