RESULTS The protein exists in Neophocaena asiaeorientalis File available The protein does not exist in Neophocaena asiaeorientalis File not available Protein Scaffold Gene location Tblastn Exons T-coffee SECIS info SECIS Seblastian Predicted protein Sel15 KZ688413.1 11431-51756 Glutathione peroxidase family GPx1 KZ686378.1 785042-785884 GPx2 KZ687437.1 914268-917392 GPx3 KZ686489.1 205068-206355 GPx4 KZ686867.1 2035805-2036866 GPx5 KZ686378.1 519485-526293 GPx6 KZ688217.1 494754-526290 GPx7 KZ686824.1 5154138-5160331 GPx8 KZ688285.1 7740822-7745085 Iodothyronine deiodinase family DI1 KZ686824.1 6378358-6389984 DI2 KZ686724.1 109076-118197 DI3 KZ686590.1 7598239-7599036 MsrA KZ687250.1 784273-1157523 SelH KZ687389.1 11572076-11572598 SelI KZ688256.1 1939120-1976312 SelK KZ687235.1 2446417-2448040 SelM KZ688414.1 1871838-1874036 SelN KZ688383.1 3964000-3977109 SelO KZ686720.1 479701-499361 SelP KZ686890.1 1533823-1538970 Selenoprotein R family SelR1 KZ687977.1 1695603-1697931 SelR2 KZ686645.1 3712242-3724509 SelR3 KZ686645.1 16747508-16853237 SelS KZ688257.1 169717-177377 SelT KZ687677.1 57301-57885 Selenoprotein U family SelU1 KZ686884.1 2812631-2822284 SelU2 KZ688101.1 340620-350879 SelU3 KZ688031.1 2725804-2727629 SelV - - Selenoprotein W family SelW1 KZ688431.1 3253694-3253768 SelW2 KZ686466.1 2167618-2168518 Thioredoxin reductase family TR1 KZ688579.1 3576884-3623111 TR2 KZ688579.1 6256779-6283812 TR3 KZ688579.1 2969431-2997701 Selenoproteins machinery Protein Scaffold Gene location Tblastn Exons T-coffee SECIS info SECIS Seblastian Predicted protein eEFsec KZ688166.1 1849532-1936606 PSTK KZ687414.1 1390-4718 SBP2 KZ688550.1 960209-997588 SecS KZ687172.1 2503280-2537885 SEPHS1 KZ687148.1 1525204-1526403 SECp43 KZ688383.1 6138844-6159934 SPS1 KZ686361.1 1370782-1392133 SPS2 KZ687148.1 1365930-1392109 Phylogenetic tree Figure 7. Phylogenetic tree comparing the selenoproteosome of Homo sapiens with the the selenoproteosome of Neophocaena asiaeorientalis This is the phylogenetic tree obtained using the program Phylogeny.fr. which compares the homology of the selenoprotein of Homo sapiens with the Neophocaena asiaeorientalis ones. In this image we can observe that the different families of selenoproteins, such as the GPxs family, are found in clusters. Considering the homology between the two species, proteins in Homo sapiens are indeed phylogenetically close with the proteins predicted. In some particular cases, for example in PSTK or SelO, the protein predicted is phylogenetically distant from the ones of Homo sapiens.