We can conclude that 18 selenoproteins were identified in Mus spicilegus. They fit into the requisites to be a selenoprotein because they start with methionine, they have a selenocysteine and a SECIS structure in the region 3’UTR. These proteins are: GPX1, GPX2, GPX3, GPX4, Sel15, SelH, SelI, SelM, SelN, SelS, TR1, TR2, TR3, DIO1, DIO3, SelenoO, SelenoP, MSRB1.
Also 7 machinery proteins were found. These are: SecS, eEFsec, PSTK, SECp43, SBP2, SPS1 and SPS2 but only SPS2 is a selenoprotein, as the other ones do not have a selenocysteine residue. These proteins are Cysteine-containing homologous.
All of them were pretty conserved, showing a T-coffee score of 1000 comparing it with Mus musculus.
Some of the proteins analysed might have reversed Selenocysteine into Cysteine. We have considered that they might have conserved its function although they are not considered selenoproteins. These are GPX5, GPX6, GPX7, GPX8, MsrA, SelU1, SelU2, SelU3 and MSRB2.
In some cases it has been found that the protein predicted does not start with a methionine, meaning that maybe the predictions only included part of the protein. These ones are: SelW1, GPX5, MSRB2, MSRB3, eEFsec, SECp43. Further research is needed in order to correctly confirm the start with methionine of these proteins.
These results mainly concur with what was seen in literature.