ABSTRACT
The main objective of this study was to identify the genes that set up the selenoproteome of Oncorhynchus mykiss (commonly known as rainbow trout), a salmonid native from Asia and North America.
The selenoproteome is the group of all the selenoproteins present in an organism. Selenoproteins are proteins that contain one or more selenocysteines (Sec) in their primary sequences. Sec residue is codified by the UGA codon, which normally acts as a stopping translation signal. Incorporation of Sec instead of the translation termination is achieved by a specific machinery that intercedes in the common process. For this reason, and for achieving our main objective, we have also analyzed the genomic regions related to the selenoprotein translational machinery.
In order to correctly predict and assess the Oncorhynchus mykiss selenoproteome and the proteins related to it synthesis, we have compared its genome with Danio rerio selenoproteome acquired from SelenoDB.
In our study we have predicted 59 selenoproteins, 12 Cys-containing homologous and 11 selenoprotein translation machinery proteins. These results adjust to what was expected due to the recent whole-genome duplication that occurred in salmonids known as Ssr4.
