Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|70870239|gb|AAHK01001632.1|:subseq(1423,2260) Trypanosoma
cruzi strain CL Brener tcruzi_1047053508727, whole genome shotgun
sequence:[translate(1)]
(89 letters)
Database: genome.fa
1696 sequences; 31,706,682 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
mex120l07.p1k 64 2e-11
mex205b01.q1k 29 0.59
mex208m22.q1k 28 0.77
mex207h05.p1k 28 1.0
mex380f18.p1k 27 1.7
mex158p21.q1k 27 2.2
mex281p19.q1k 27 2.2
mex345c16.q1k 27 2.2
mex584j22.q1k 27 2.9
mex384j17.q1k 27 2.9
mex527e14.p1k 27 2.9
mex430g05.q1k 26 3.8
mex318i07.p1k 26 3.8
mex229h17.q1k 26 3.8
mex369l15.p1k 26 3.8
mex546l16.p1k 26 5.0
mex274n07.q1k 26 5.0
mex69f05.p1k 26 5.0
mex427i20.p1k 26 5.0
mex175g18.p1k 25 6.5
mex298l01.q1k 25 6.5
mex106a04.p1k 25 6.5
mex121b19.q1k 25 6.5
mex160k05.q1k 25 6.5
mex454c03.q1k 25 6.5
mex336f02.q1k 25 6.5
mex173e13.q1k 25 8.5
mex416b20.q1k 25 8.5
mex115n07.q1k 25 8.5
mex281i24.q1k 25 8.5
mex126c12.q1k 25 8.5
>mex120l07.p1k
Length = 38952
Score = 63.5 bits (153), Expect = 2e-11
Identities = 39/117 (33%), Positives = 60/117 (51%), Gaps = 30/117 (25%)
Frame = -1
Query: 1 MPYVANGRVVDSKPFSLVDFFLSILRLMWLFFATLVSSEPVSQHVQEYR----------- 49
M Y+ NGRVV+ KPF++ F L++ + LF TL +++P+ Q V+E++
Sbjct: 30279 MAYIHNGRVVEKKPFTVQGFLLALWTALSLFAQTLFTTQPIGQVVEEHQHPAPRAANAAA 30100
Query: 50 ------STTRIV---------PFGGGRSVGHAP----RGPNIHGLPKGSLTGGCATG 87
+ R + GGGR+ G A RG N+H LPK ++GGCA+G
Sbjct: 30099 DGGGILGSLRAMLNRRGGGGQALGGGRNSGWAAAVNRRGGNVHTLPKAPISGGCASG 29929
>mex205b01.q1k
Length = 18965
Score = 28.9 bits (63), Expect = 0.59
Identities = 18/53 (33%), Positives = 21/53 (39%)
Frame = +2
Query: 34 TLVSSEPVSQHVQEYRSTTRIVPFGGGRSVGHAPRGPNIHGLPKGSLTGGCAT 86
T VS P + + T R P G RS G G HG P + T C T
Sbjct: 9194 TRVSRAPCQRQARRLARTCRRPPPLGKRSSGPRATGREFHGSPARAATSICRT 9352
>mex208m22.q1k
Length = 81898
Score = 28.5 bits (62), Expect = 0.77
Identities = 18/44 (40%), Positives = 22/44 (50%), Gaps = 8/44 (18%)
Frame = +2
Query: 52 TRIVPF-----GGGRSVGHAPRGPNIHGLPKGSLTG---GCATG 87
+R+ PF GGG G APRGP I P + T GC+ G
Sbjct: 53171 SRVSPFFWGGGGGGLLGGEAPRGPVIAAFPPPAGTAEMVGCSRG 53302
>mex207h05.p1k
Length = 42588
Score = 28.1 bits (61), Expect = 1.0
Identities = 15/53 (28%), Positives = 26/53 (49%)
Frame = +1
Query: 35 LVSSEPVSQHVQEYRSTTRIVPFGGGRSVGHAPRGPNIHGLPKGSLTGGCATG 87
++S+EP+ ++ + T+ + G S GH RG GL + + G TG
Sbjct: 41041 IISAEPIHAASRKIKFGTKAITLGKSTSQGH-KRGTAARGLKEKRASSGVKTG 41196
>mex380f18.p1k
Length = 169514
Score = 27.3 bits (59), Expect = 1.7
Identities = 17/50 (34%), Positives = 21/50 (42%)
Frame = +1
Query: 40 PVSQHVQEYRSTTRIVPFGGGRSVGHAPRGPNIHGLPKGSLTGGCATGXR 89
P+S+ Q R T+ P GGGRS RG + G GC R
Sbjct: 97102 PLSEE*Q-IRRTSGARPSGGGRSCARVHRGLRVRGRRVMDSVAGCCESRR 97248
Score = 26.6 bits (57), Expect = 2.9
Identities = 15/34 (44%), Positives = 17/34 (50%), Gaps = 5/34 (14%)
Frame = -1
Query: 58 GGGRSV-----GHAPRGPNIHGLPKGSLTGGCAT 86
GGGR G+ RG N G+P GS G C T
Sbjct: 68429 GGGRGKPR*RRGNLIRGSNGPGVPCGSRAGACVT 68328
>mex158p21.q1k
Length = 50488
Score = 26.9 bits (58), Expect = 2.2
Identities = 14/32 (43%), Positives = 15/32 (46%)
Frame = +3
Query: 58 GGGRSVGHAPRGPNIHGLPKGSLTGGCATGXR 89
GGGR G RG GL G + G C G R
Sbjct: 5586 GGGRGRGGLRRGDAGTGLHPGRIEGSCKHGQR 5681
>mex281p19.q1k
Length = 20349
Score = 26.9 bits (58), Expect = 2.2
Identities = 16/50 (32%), Positives = 20/50 (40%)
Frame = -3
Query: 40 PVSQHVQEYRSTTRIVPFGGGRSVGHAPRGPNIHGLPKGSLTGGCATGXR 89
P HV +R T G + PRGP P+G+ G C G R
Sbjct: 4324 PFVAHVLRHRPRTET----SGCTARRHPRGPRSEPGPRGAAGGVCRAGSR 4187
>mex345c16.q1k
Length = 22995
Score = 26.9 bits (58), Expect = 2.2
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +1
Query: 57 FGGGRSVGHAPRGPNIHGLPKG 78
+GGG G PRGPN G P+G
Sbjct: 20329 WGGGSGGGQMPRGPNFPG-PRG 20391
>mex584j22.q1k
Length = 52536
Score = 26.6 bits (57), Expect = 2.9
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = -1
Query: 67 PRGPNIHGLPKGSLTG 82
PRGP +HG +GS TG
Sbjct: 858 PRGPRLHGDQRGSSTG 811
Score = 25.4 bits (54), Expect = 6.5
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -2
Query: 62 SVGHAPRGPNIHGLPKGSLTGGC 84
++GH+ + P H LP+ GGC
Sbjct: 36770 ALGHSGKDPAAHRLPRHVRAGGC 36702
>mex384j17.q1k
Length = 78339
Score = 26.6 bits (57), Expect = 2.9
Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 2/45 (4%)
Frame = +1
Query: 37 SSEPVSQHVQEYRSTTRIVPFGGGRSVGHAPRGPNIH--GLPKGS 79
S+ P+ H+ E P GGG + PRG ++ GLP G+
Sbjct: 71572 SAVPLPLHLLEGPDPDLRAPLGGGEAATAGPRGVPLYVEGLPGGA 71706
>mex527e14.p1k
Length = 82782
Score = 26.6 bits (57), Expect = 2.9
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = +1
Query: 16 SLVDFFLSILRLMWLFFATLVSSEPVS 42
S+ FF S+L L+WLFF +L S P +
Sbjct: 23173 SVASFFFSML-LLWLFFCSLPFSIPTT 23250
>mex430g05.q1k
Length = 67171
Score = 26.2 bits (56), Expect = 3.8
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 58 GGGRSVGHAPRGPNIHGLPKGSLTGGCATG 87
GGG +GH R P++ P G+L G A G
Sbjct: 59583 GGGPVLGHLRRAPSLSS-PTGTLIPGSARG 59669
>mex318i07.p1k
Length = 75510
Score = 26.2 bits (56), Expect = 3.8
Identities = 15/40 (37%), Positives = 18/40 (45%), Gaps = 1/40 (2%)
Frame = -3
Query: 42 SQHVQ-EYRSTTRIVPFGGGRSVGHAPRGPNIHGLPKGSL 80
S HVQ R+ R GGG + GPN+H G L
Sbjct: 43567 SVHVQGSLRAAVRSAAQGGGVQLAAPHDGPNVHNPTSGGL 43448
>mex229h17.q1k
Length = 31059
Score = 26.2 bits (56), Expect = 3.8
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +2
Query: 37 SSEPVSQHVQEYRSTTRIVPFGGGRSVG 64
+SE + HV + S T IVPF GGR+ G
Sbjct: 10553 ASEWPNLHVVAHLSITFIVPFCGGRTGG 10636
>mex369l15.p1k
Length = 34167
Score = 26.2 bits (56), Expect = 3.8
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +2
Query: 59 GGRSVGHAPRGPNIHGLPK 77
GG + P+ PNIHG+P+
Sbjct: 29549 GGDCLARHPQSPNIHGVPQ 29605
>mex546l16.p1k
Length = 7990
Score = 25.8 bits (55), Expect = 5.0
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = -2
Query: 58 GGGRSVGHAPRGPNIHG 74
GG +S G P GP +HG
Sbjct: 1296 GGAKSTGDNPNGPVVHG 1246
>mex274n07.q1k
Length = 17642
Score = 25.8 bits (55), Expect = 5.0
Identities = 14/34 (41%), Positives = 16/34 (47%), Gaps = 4/34 (11%)
Frame = +3
Query: 60 GRSVGHAPRGPNIHGLP----KGSLTGGCATGXR 89
GRS PR P+ HGL + CATG R
Sbjct: 12030 GRSAAVLPRPPSSHGLAAVCGSREASAACATGRR 12131
>mex69f05.p1k
Length = 106748
Score = 25.8 bits (55), Expect = 5.0
Identities = 11/28 (39%), Positives = 13/28 (46%)
Frame = -3
Query: 49 RSTTRIVPFGGGRSVGHAPRGPNIHGLP 76
RS ++ GG R H P PN H P
Sbjct: 39087 RSAATVMKGGGRRHESHVPPTPNTHHAP 39004
>mex427i20.p1k
Length = 28368
Score = 25.8 bits (55), Expect = 5.0
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +1
Query: 47 EYRSTTRIVPFGGGRSVGHAPRGPNIHGLP 76
+YR R G GR +G P P HG+P
Sbjct: 16573 QYRRHIRRTGEGDGRHLGR*PGAPVAHGVP 16662
>mex175g18.p1k
Length = 39072
Score = 25.4 bits (54), Expect = 6.5
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -1
Query: 53 RIVPFGGGRSVGHAPRGPNIHGLPKGSLTGGCATGXR 89
R++ GGG +V A P +HG + + G AT R
Sbjct: 6540 RVLSEGGGAAVVEASAAPTVHG-QRQRRSSGAATSPR 6433
>mex298l01.q1k
Length = 110435
Score = 25.4 bits (54), Expect = 6.5
Identities = 13/26 (50%), Positives = 14/26 (53%)
Frame = +3
Query: 49 RSTTRIVPFGGGRSVGHAPRGPNIHG 74
RS R + G GRS G AP IHG
Sbjct: 44013 RSFCRRLARGDGRSCGAAPHAAPIHG 44090
>mex106a04.p1k
Length = 259109
Score = 25.4 bits (54), Expect = 6.5
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = +3
Query: 61 RSVGHAPRGPNIHGLPKGSLTGGC 84
R HAP+ N L + TGGC
Sbjct: 41178 RPTAHAPQTQNFSALSSSTSTGGC 41249
>mex121b19.q1k
Length = 60457
Score = 25.4 bits (54), Expect = 6.5
Identities = 11/22 (50%), Positives = 14/22 (63%), Gaps = 2/22 (9%)
Frame = +2
Query: 65 HAPRGPNIHGLPKGSLTG--GC 84
H PRG +H LP+ S+ G GC
Sbjct: 45806 HRPRGAVLHRLPRDSVNGCSGC 45871
>mex160k05.q1k
Length = 48265
Score = 25.4 bits (54), Expect = 6.5
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -3
Query: 40 PVSQHVQEYRSTTRIVPFGGGRSVGHAPRGPNI--HGLPK 77
PVSQ + R R+V G G + GPNI HG P+
Sbjct: 31109 PVSQGGRRQRRDGRVVGVGVPDIRGRSTPGPNIRHHGTPQ 30990
>mex454c03.q1k
Length = 27867
Score = 25.4 bits (54), Expect = 6.5
Identities = 13/24 (54%), Positives = 15/24 (62%), Gaps = 3/24 (12%)
Frame = +2
Query: 42 SQHVQEY---RSTTRIVPFGGGRS 62
SQ Q Y STTR+VPF G R+
Sbjct: 8294 SQRYQRYSSCNSTTRVVPFCGARA 8365
>mex336f02.q1k
Length = 33634
Score = 25.4 bits (54), Expect = 6.5
Identities = 15/38 (39%), Positives = 18/38 (47%), Gaps = 2/38 (5%)
Frame = +2
Query: 49 RSTTRIVPFGGG--RSVGHAPRGPNIHGLPKGSLTGGC 84
RST R+ G +S+ H PR P I P GGC
Sbjct: 21047 RSTQRLRHVVGSTKQSLRHIPRTPQIPRAPPSCSQGGC 21160
>mex173e13.q1k
Length = 10494
Score = 25.0 bits (53), Expect = 8.5
Identities = 9/23 (39%), Positives = 12/23 (52%)
Frame = +2
Query: 58 GGGRSVGHAPRGPNIHGLPKGSL 80
GGGR+ H P HG G++
Sbjct: 9848 GGGRTASHPPHAAKDHGHTNGNM 9916
>mex416b20.q1k
Length = 58341
Score = 25.0 bits (53), Expect = 8.5
Identities = 18/47 (38%), Positives = 19/47 (40%), Gaps = 1/47 (2%)
Frame = +1
Query: 40 PVSQHVQEYRSTTR-IVPFGGGRSVGHAPRGPNIHGLPKGSLTGGCA 85
P S RS R + PF GG V AP GP G GCA
Sbjct: 1645 PSSSSSSSTRSRRRELPPFSGGGCVCEAPLGP-------GKREAGCA 1764
>mex115n07.q1k
Length = 110320
Score = 25.0 bits (53), Expect = 8.5
Identities = 12/30 (40%), Positives = 13/30 (43%)
Frame = +2
Query: 58 GGGRSVGHAPRGPNIHGLPKGSLTGGCATG 87
G G VGH + L L GGCA G
Sbjct: 21941 GAGECVGHVAHRARLLPLQLQRLHGGCAAG 22030
>mex281i24.q1k
Length = 22396
Score = 25.0 bits (53), Expect = 8.5
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 5/34 (14%)
Frame = +1
Query: 23 SILRLMWLFFATLVSSEPV-----SQHVQEYRST 51
S + +WLFFA L S +PV + V Y ST
Sbjct: 18292 SFVAKLWLFFAFLQSCQPVIWGSQQRQVPTYAST 18393
>mex126c12.q1k
Length = 17091
Score = 25.0 bits (53), Expect = 8.5
Identities = 20/64 (31%), Positives = 24/64 (37%), Gaps = 16/64 (25%)
Frame = +2
Query: 37 SSEPVSQHVQEYRSTTRIVPFGGGRSVGHAP------------RGPNIHGL----PKGSL 80
S+ P YRS R GGG + H P RG + GL P +L
Sbjct: 10007 STHPFRTQANAYRSKGRGWEDGGGLVLPHLPLLAQAR*CSLRGRGVCVQGLRGDEPTSTL 10186
Query: 81 TGGC 84
TG C
Sbjct: 10187 TGDC 10198
Database: genome.fa
Posted date: Jan 16, 2009 12:51 PM
Number of letters in database: 31,706,682
Number of sequences in database: 1696
Lambda K H
0.323 0.141 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,157,653
Number of Sequences: 1696
Number of extensions: 64499
Number of successful extensions: 628
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 338
Number of HSP's successfully gapped in prelim test: 28
Number of HSP's that attempted gapping in prelim test: 232
Number of HSP's gapped (non-prelim): 455
length of query: 89
length of database: 10,568,894
effective HSP length: 64
effective length of query: 25
effective length of database: 10,460,350
effective search space: 261508750
effective search space used: 261508750
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 53 (25.0 bits)
Torna a dalt