Discovering Gavialis gangeticus selenoproteins

DI1 protein is located between 420389 and 425977 positions of the KN323949.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 65%, an e-value of 3x10^-34 and was found in the positive strand.

The predicted protein contains 4 exons and 3 introns and we have found a Sec residue in exon 2. This residue has been conserved in chicken, human and gavial.

Finally, we located a SECIS element of grade A between positions 427398 and 427461 in the positive strand.

DI2

DI2 protein is located between positions 21508 and 33230 of the KN327708.1 scaffold . The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 90%, an e-value of 10^-112 and it was found in the positive strand.

The predicted protein contains 2 exons and 1 intron and we have found a Sec residue in exon 2. This residue has been conserved in chicken, human and gavial.

Finally, we have found a SECIS element of grade A between positions 38338 and 38410.

DI3

DI3 protein is located between 37015 and 102707 positions of the KN334575.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 60%, an e-value of 8x10^-90 and was found in the positive strand.

As we can see, we could not predict the whole protein because it begins with leucine instead of methionine. However, the predicted protein contains 6 exons and 5 introns and we have found a Sec residue in exon 6. This residue has been conserved in chicken, human and gavial.

Finally, we have found a SECIS element of grade A between positions 102782 and 102 861 in the positive strand.

GPx1

GPx1 protein is located between 24515 and 30340 positions of the KN325303.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 62% an e-value of 2x10^-54 and was found in the negative strand.

As we can observe, we could not predict the whole protein because it begins with alanine instead of methionine. However, the predicted protein contains 2 exons and 1 intron and we have found a Sec residue in exon 1. This residue has been conserved between human and gavial but there is no data for GPx1 protein in SelenoDB for chicken genome. Assuming that this selenoprotein or selenocysteine residue was lost during evolution in chicken, it is possible that the common ancestor between chicken and gavial had this selenoprotein and was conserved in gavial but not in chicken.

Finally, we have found a SECIS element of grade A between positions 24285 and 24352 in the negative strand.

GPx2

GPx2 protein is located between positions 34981 and 37356 of the KN332733.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 72% an e-value of 2x10^-54 and was found in the positive strand.

The predicted protein contains 2 exons and 1 intron and we have found a Sec residue in exon 1. This residue has been conserved between human and gavial but there is no data for GPx2 protein in SelenoDB for chicken genome. Assuming that this selenoprotein was lost during evolution in chicken, it is possible that the common ancestor between chicken and gavial had this selenoprotein and was conserved in gavial but not in chicken.

Finally, we have found a SECIS element of grade A between positions 37484 and 37551 of the positive strand.

GPx3

GPx3 protein is located between positions 11948 and 38921 of the KN325192.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 53 %, an e-value of 3x10^-28 and was found in the positive strand.

The predicted protein contains 5 exons and 4 introns and we have found a Sec residue in exon 2. This residue has been conserved in chicken, human and gavial.

Finally, we have found a SECIS element of grade A between positions 39910 and 39981 of the positive strand.

Sel15

Sel15 protein is located between positions 83143 and 130918 of the KN331551.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 82%, an e-value of 5x10^-19 and is in the negative strand.

We have not been able to predict the complete protein because we see that in T-coffee alignment is not complete and does not start with methionine. This may be due to the scaffold ending before we could get the complete protein. However, our prediction contains 4 exons and 3 introns and we have found a Sec residue in exon 2. This residue was conserved by human, chicken and gavial.

Finally, we found a SECIS element of grade A between positions 82467 and 82514 of the negative strand.

SelH

SelH protein is located between positions 17064 and 18112 of the KN331620.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 63%, and e-value of 3x10^-11 and was found in the negative strand.

The predicted protein contains 4 exons and 3 introns and we have found a Sec residue in exon 3. This residue has been conserved between human and gavial but is substituted for a cysteine in chicken's genome. It is possible that the common ancestor between chicken and gavial had this selenocysteine and was conserved in gavial but not in chicken.

Finally, we found a SECIS element of grade A between positions 15872 and 15951 of the negative strand.

SelI

SelI protein is located between positions 5267 and 17947 of the KN340711.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 64%, an e-value of 2x10^-37 and was found in the positive strand.

We could not predict the protein completely. As we can see, in the alignment obtained thorugh T-coffee is not complete and does not start with methionine. This is probably because the scaffold begins after the start of the predicted protein so that it is not found in the studied scaffold. However, the predicted protein contains 7 exons and 6 introns and we have found a Sec residue in exon 7. This residue is conserved in human, chicken and gavial.

Finally, found SECIS element of grade A between positions 19240 and 19316 of the positive strand.

SelK

SelK protein is located between positions 2714 and 5998 of the KN339408.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 80%, an e-value of 4x10^-6 and was found in the negative strand.

The predicted protein contains 4 exons and 3 introns and we have found a Sec residue in exon 4. This residue is conserved in human, chicken and gavial.

Finally, we found a SECIS element of grade A between positions 1088 and 1166 in the negative strand.

SelM

SelM protein is located between positions 208 and 5648 of the KN331620.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 73% an e-value of 4x10^-13 and was found in the positive strand.

We could not predict the complete protein. As we can see, the T-coffee alignment is not complete and the predicted protein does not start with methionine. However, the predicted protein contains 5 exons and 4 introns and we have found a Sec residue in exon 2. This residue has been conserved between human and gavial but there is no data for SelM protein in SelenoDB for chicken genome. Assuming that this selenoprotein or selenocisteine residue was lost during evolution in chicken, it is possible that the common ancestor between chicken and gavial had this selenoprotein and was conserved in gavial but not in chicken.

Finally, we found a SECIS element of grade A between positions 6376 and 6449 of the positive strand.

SelN

SelN protein is located between the positions 93464 and 108939 of the KN323949.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 86%, an e-value of 7x10^-33 and was found in the negative strand.

Despite we could not predict the whole protein, because it does not start with a methionine residue, the part which have been predicted has 12 exons and 11 introns, with the Sec residue in the 1st exon and another one in the 9th. The residue from the 9th exon is conserved in chicken, human and gharial, but the residue from the 1st exon is only found in gharial and human (1 & 2). It could be possible that the last ancestor between gharial, chicken and human had the residue, but chicken might lost it.

Finally, we have localized a SECIS element with an A grade between the positions 39807 and 39877 of the negative strand.

SelO

SelO protein is located between the positions 189095 and 198355 of the KN323317.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 62%, an e-value of 1x10^-28 and was found in the negative strand.

Despite we could not predict the whole protein, because it does not start with a methionine residue, the part which have been predicted has 8 exons and 7 introns, with the Sec residue in the 8th exon. The residue is conserved in chicken, human and gharial.

Finally, we have localized a SECIS element with an B grade between the positions 73885 i 73931 of the negative strand.

SelP

SelP protein is located between the positions 30419 and 37131 of the KN331503.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 86%, an e-value of 2x10^-28 and was found in the negative strand.

The predicted protein has 4 exons and 3 introns, with the Sec residue in the 1st exon and. The residue is conserved in chicken, human and gharial. Furthermore, human isoforms of the protein have between 1 to 9 more Sec residues, so it is probable that those had appeared after the evolutive separation of mammals and reptiles branches.

Finally, we have localized a SECIS element with an A grade between the positions 39807 and 39877 of the negative strand.

SelR1

SelR1 protein is located between the positions 9859 and 54028 of the KN328135.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 62%, an e-value of 2x10^-41 and was found in the negative strand.

Despite we could not predict the whole protein, because it does not start with a methionine residue, the part which have been predicted has 8 exons and 7 introns, with the Sec residue in the 3rd exon. It is also probable that V of our predicted protein works as an starting codon (aligned with M in T-coffee). The residue is conserved in chicken, human and gharial.

Finally, we have localized a SECIS element with an B grade between the positions 48563 and 48635 of the negative strand.

SelS

SelS protein is located between the positions 174094 and 180838 of the KN343631.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 53%, an e-value of 1x10^-7 and was found in the negative strand.

Despite we could not predict the whole protein, because it does not start with a methionine residue, the part which have been predicted has 6 exons and 5 introns, with the Sec residue in the 6th exon. The residue is conserved in chicken, human and gharial.

Finally, we have localized a SECIS element with an A grade between the positions 45391 and 45479 of the negative strand.

SelT

SelT protein is located between the positions 25580 and 33162 of the KN332697.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 87%, an e-value of 8x10^-21 and was found in the positive strand.

Despite we could not predict the whole protein, because it does not start with a methionine residue, the part which have been predicted has 5 exons and 4 introns, with the Sec residue in the 2nd exon. The residue is conserved in chicken, human and gharial.

Finally, we have localized a SECIS element with an A grade between the positions 37946 and 38027 of the positive strand.

SelU1

SelU1 protein is located between the positions 84072 and 92083 of the KN328551.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 75%, an e-value of 9x10^-20 and was found in the positive strand.

The predicted protein has 5 exons and 4 introns, with the Sec residue in the 2nd exon. The residue is conserved in chicken and gharial, but not in humans. It is probable that the residue had disappeared after the evolutive separation of mammals and reptiles branches.

Finally, we have localized a SECIS element with an A grade between the positions 58215 and 58282 of the positive strand.

SPS2

SPS2 protein is located between the positions 4433 and 26094 of the KN325147.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 85%, an e-value 2x10^-23 and was found in the positive strand.

Despite we could not predict the whole protein, because it does not start with a methionine residue, the part which have been predicted has 9 exons and 8 introns, with the Sec residue in the 2nd exon. The residue is conserved in human and gharial, but not in chicken, so it is probable that the last ancestor between the chicken and gharial had a Sec residue.

Finally, we have localized a SECIS element with an A grade between the positions 26555 and 26632 of the negative strand.

TR1

TR1 protein is located between the positions 102597 and 152094 of the scaffold KN333725.1. The hit given by tblastn was obtained from comparison with Homo sapiens, has an identity of 70%, an e-value of 2x10^-17 and was found in the negative strand.

We could not predict the whole protein, as can we see in the alignment of T-coffee, because the beginning of the alignment is short for the predicted protein. This is because the scaffold is not long enough to contain the whole protein. However, the predicted protein contains 18 exons and 17 introns and we have located a Sec residue aligned with the human Sec residue in exon 18 and a residue Sec not aligned with a human one in the exon 2. Selenocysteine of exon 18 is maintained in human and gharial but we have no data for TR1 in chicken. Assuming that the protein was lost in the chicken during evolution it is posible that the common ancestor between chicken and gharial had this protein but was only conserved in gharial.

Finally, we were able to locate a grade A SECIS element between positions 99394 and 99468 of the negative strand.

TR2

TR2 protein is located between the positions 4231 and 54706 of the KN346354.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 63%, an e-value of 9x10^-20 and was found in the positive strand.

Despite we could not predict the whole protein, because it does not start with a methionine residue, the part which have been predicted has 14 exons and 13 introns, with the Sec residue in the 14th exon. The residue is conserved in human, chicken and gharial.

Finally, we have localized a SECIS element with an A grade between the positions 3903 and 3978 of the positive strand.

TR3

TR3 protein is located between the positions 57360 and 89451 of the KN347796.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 67%, an e-value 2x10^-54 and was found in the positive strand.

The predicted protein has 16 exons and 15 introns, with the Sec residue in the 16th exon. The residue is conserved in chicken, gharial, and humans.

Finally, we have localized a SECIS element with an A grade between the positions 82314 and 82388 of the positive strand.

Machinery Discussion

eEFSec

eEFSec protein is located between 1657 and 50943 positions of the KN328583.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 89%, an e-value of 10^-127 and was found in the positive strand.

We could not predict the complete protein because, as we can see, it starts with a serine not with a methionine residue. The predicted protein contains 4 exons and 3 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that eEFSec, as in chicken, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

EIF4A3

EIF4A3 protein is located between 9377 and 23227 positions of the KN353580.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 97%, an e-value of 10^-36 and was found in the positive strand.

We could not predict the complete protein because, as we can see, it starts with a serine not with a methionine residue. The predicted protein contains 12 exons and 11 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that EIF4A3, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

MsrA

MsrA protein is located between 43319 and 75367 positions of the KN339230.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 87%, an e-value of 2x10^-27 and was found in the negative strand.

We could not predict the complete protein because, as we can see, it starts with a lysin not with a methionine residue. The predicted protein contains 2 exons and 1 intron and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that MsrA, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

PSTK

PTSK protein is located between 25987 and 30084 positions of the KN326215.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 57%, an e-value of 6x10^-16 and was found in the positive strand.

We could not predict the complete protein because, as we can see, it starts with a serine not with a methionine residue. The predicted protein contains 8 exons and 7 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that PTSK, as in chicken, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

RPL30

RPL30 protein is located between 9571 and 10617 positions of the KN359763.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 100%, an e-value of 3x10^-23 and was found in the positive strand.

The predicted protein contains 2 exons and 1 intron, and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that RPL30, as in chicken and human, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

SARS2

SARS2 protein is located between 3729 and 18387 positions of the KN325240.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 87%, an e-value of 7x10^-11 and was found in the positive strand.

We could not predict the complete protein because, as we can see, it starts with a cysteine not with a methionine residue. The predicted protein contains 18 exons and 17 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that SARS2, as in human, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

SBP2

SBP2 protein is located between 2 and 24332 positions of the KN340409.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 67%, an e-value of 2x10^-76 and was found in the positive strand.

We could not predict the complete protein because, as we can see, it starts with a threonine not with methionine. The predicted protein contains 16 exons and 15 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that SBP2, as in chicken, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

SECp43

SECp43 protein is located between 9110 and 85237 positions of the KN351604.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 73%, an e-value of 2x10^-9 and was found in the positive strand.

We could not predict the complete protein because, as we can see, it starts with a leucine not with a methionine residue. The predicted protein contains 13 exons and 12 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that SECp43, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

SecS

SecS protein is located between 10684 and 59440 positions of the KN341850.1 scaffold. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 98%, an e-value of 2x10^-42 and was found in the positive strand.

The predicted protein contains 11 exons and 10 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that SecS, as in chicken, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

SEPSECS

SEPSECS protein is located between 10486 and 59440 positions of the KN341850.1 scaffold. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 94%, an e-value of 6x10^-41 and was found in the negative strand.

The predicted protein contains 11 exons and 10 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that SEPSECS, as in chicken, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

SPS1

SPS1 protein is located between 172655 i 201393 positions of the KN331553.1. The hit found by tblastn was obtained by comparison with Gallus gallus, has an identity of 98%, an e-value of 5x10^-38 and was found in the positive strand.

The predicted protein contains 7 exons and 6 introns and we have not found any Sec residue. We have not located any SECIS element.

With this results we can conclude that SPS1, as in chicken, is not a selenoprotein and is part of the synthesis machinery of selenoproteins.

Cys-homologues Discussion

CELF1

CELF1 protein is located between positions 67322 and 148262 of the KN326986.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 78% an e-value of 8x10^-26 and was found in the positive strand.

The predicted protein contains 8 exons and 7 introns without Sec residues in its sequence. In spite of the fact that it starts with methionine, we have not been able to predict the complete protein because we see that T-coffee alignment is short and incomplete.

Finally, we found a SECIS element of grade B between positions 81401 and 81469.

ELAVL1

ELAVL1 protein is located between positions 2324 and 100675 of the KN326208.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 100% an e-value of 2x10^-66 and was found in the positive strand.

The predicted protein contains 5 exons and 4 introns without Sec residues in its sequence. In spite of the fact that it starts with methionine, we observe that query sequence presents gaps at the beginning and at the end. Hence, the alignment is incomplete.

Finally, we found a SECIS element of grade B between positions 81923 and 82006.

G6PD

G6PD protein is located between positions 29 and 3675 of the KN354991.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 83% an e-value of 6x10^-56 and was found in the positive strand.

The predicted protein contains 9 exons and 8 introns without Sec residues in its sequence. We have not been able to predict the complete protein because we see that T-coffee alignment is not complete and does not start with methionine. This may be due to the scaffold ending before we could get the complete protein.

Finally, any SECIS element located in the 3’ UTR region has been found.

GPx4

GPx4 protein is located between the positions 167048 and 178702 of the scaffold KN350882.1. The hit found by tblastn was obtained by comparison with Homo sapiens, has an identity of 72%, an e-value of 4x10^-18 and was found in the negative strand.

As we can see, we could not predict the whole protein because it starts with arginine instead of methionine. However, the predicted protein contains seven exons and six introns and we have found a Sec residue in exon 3. This residue has been conserved between human and gavial but there is no data for GPx4 protein in SelenoDB for chicken genome. Assuming that this selenoprotein or selenocisteine residue was lost during evolution in chicken, it is possible that the common ancestor between chicken and gavial had this selenoprotein and was conserved in gavial but not in chicken.

Finally, we could not find any SECIS element. Therefore, we can not consider GPx4 as a selenoprotein in chicken because Sec can not be incorporated during translation without a SECIS element. However, the problem could be that Seblastian can not find this structure because it works by comparing with know selenoproteins and SECIS elements of other species.

GPx7

GPx7 protein is located between positions 4518 and 8341 of the KN343115.1 scaffold. The hit taken by tblastn was obtained by comparison with Gallus gallus, has an identity of 87% an e-value of 1x10^-41 and was found in the positive strand.

The predicted protein contains 3 exons and 2 introns without Sec residues in its sequence. We have not been able to predict the complete protein because we see that T-coffee alignment is not complete and does not start with methionine. This may be due to the scaffold ending before we could get the complete protein.

Finally, any SECIS element located in the 3’ UTR region has been found.

GPx8

GPx8 protein is located between positions 105789 and 108921 of the KN325713.1 scaffold. The hit taken by tblastn was obtained by comparison with Gallus gallus, has an identity of 90% an e-value of 4x10^-48 and was found in the positive strand.

The predicted protein contains 3 exons and 2 introns without Sec residues in its sequence. It starts with methionine and it is well aligned with T-coffee. Consequently, we consider that we have it all.

Finally, we found a SECIS element of grade B between positions 67963 and 68029.

SCLY

SCLY protein is located between positions 55102 and 71706 of the KN338129.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 60% an e-value of 8x10^-19 and was found in the negative strand.

The predicted protein contains 12 exons and 11 introns without Sec residues in its sequence. We have not been able to predict the complete protein because we see that T-coffee alignment is not complete and does not start with methionine. This may be due to the scaffold ending before we could get the complete protein.

Finally, we found a SECIS element of grade B between positions 28530 and 28605 of the negative strand.

SelR2

SelR2 protein is located between positions 27975 and 56239 of the KN335858.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 67% an e-value of 4x10^-15 and was found in the negative strand.

Finally, any SECIS element located in the 3’ UTR region has been found.

SelR3

SelR3 protein is located between positions 18890 and 54034 of the KN328135.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 58% an e-value of 1x10^-36 and was found in the negative strand.

The predicted protein contains 4 exons and 3 introns without Sec residues in its sequence. It does not start with methionine and, in T-coffee alignment we can observe gaps in query sequence relative to predicted.

Finally, we found a SECIS element of grade B between positions 51581 and 51653 of the negative strand.

SelU2

SelU2 protein is located between positions 15913 and 20632 of the KN342762.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 73% an e-value of 2x10^-16 and was found in the positive strand.

The predicted protein contains 6 exons and 5 introns without Sec residues in its sequence. It does not start with methionine due to the presence of an extra nucleotide. If we could remove the first alanine of the sequence, T-coffee alignment would be correct matching both methionines.

Finally, any SECIS element located in the 3’ UTR region has been found.

SelU3

SelU3 protein is located between positions 177079 and 233872 of the KN328749.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 52% an e-value of 3x10^-11 and was found in the positive strand.

The predicted protein contains 9 exons and 8 introns without Sec residues in its sequence. Even so, the predicted protein does not start with methionine and we have not been able to predict it completely.

Finally, any SECIS element located in the 3’ UTR region has been found.

TTPA

TTPA protein is located between positions 193583 and 200940 of the KN350030.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 46% an e-value of 2x10^-12 and was found in the positive strand.

The predicted protein contains 5 exons and 4 introns without Sec residues in its sequence. Even so, the predicted protein does not start with methionine and we have not been able to predict it completely.

Finally, any SECIS element located in the 3’ UTR region has been found.

XPO1

XPO1 protein is located between positions 72686 and 123403 of the KN325182.1 scaffold. The hit taken by tblastn was obtained by comparison with Homo sapiens, has an identity of 67% an e-value of 4x10^-38 and was found in the positive strand.

The predicted protein contains 24 exons and 23 introns without Sec residues in its sequence. It starts with methionine and it is well aligned with T-coffee. Consequently, we consider that we have it all.

Finally, any SECIS element located in the 3’ UTR region has been found.