Per tal de comprovar el funcionament del programa l'hem executat per a dues seqüències de les quals en coneixem l'anotació real dels exons. Observant els resultats comprovem que el programa prediu tots els exons d'ambdues seqüències.
El fitxer fasta d'aquesta seqüència és el següent: fitxer NM_006533
Primerament mostrem l'anotació real del gen. A continuació es mostren tots els exons que el programa cercaexons ha predit per la seqüència NM_006533. En color vermell es mostren els exons reals que el programa ha predit.
# annotacio del gen real NM_006533 chr19 Utr 1 70 . + . "H.sapiens" NM_006533 chr19 First 71 197 . + . "H.sapiens" NM_006533 chr19 Internal 280 413 . + "H.sapiens" NM_006533 chr19 Internal 1497 1607 . + "H.sapiens" NM_006533 chr19 Terminal 1925 1948 . + "H.sapiens" NM_006533 chr19 Utr 1949 2019 . + . "H.sapiens"
Per aquesta seqüència hem predit un total de 58 exons.
#Exons predits amb el CERCAEXONS per la sequencia NM_006533 NM_006533 cercaexons internal 65 85 5.96 + 0 NM_006533 cercaexons internal 65 85 5.09 + 1 NM_006533 cercaexons internal 65 85 4.53 + 2 NM_006533 cercaexons terminal 65 165 2.09 + 1 NM_006533 cercaexons internal 65 197 13.55 + 0 NM_006533 cercaexons internal 65 197 6.99 + 2 NM_006533 cercaexons internal 65 413 16.60 + 2 NM_006533 cercaexons internal 65 413 12.25 + 0 NM_006533 cercaexons terminal 65 460 10.48 + 0 NM_006533 cercaexons terminal 65 509 17.35 + 2 NM_006533 cercaexons first 71 85 2.63 + 0 NM_006533 cercaexons first 71 197 10.22 + 0 NM_006533 cercaexons first 71 413 8.92 + 0 NM_006533 cercaexons single 71 460 7.15 + 0 NM_006533 cercaexons first 149 197 6.68 + 0 NM_006533 cercaexons first 149 413 5.38 + 0 NM_006533 cercaexons single 149 460 3.61 + 0 NM_006533 cercaexons first 204 413 12.05 + 0 NM_006533 cercaexons single 204 509 12.80 + 0 NM_006533 cercaexons internal 246 278 5.86 - 1 NM_006533 cercaexons terminal 280 339 3.77 + 0 NM_006533 cercaexons internal 280 413 15.99 + 1 NM_006533 cercaexons internal 280 413 2.93 + 2 NM_006533 cercaexons terminal 280 509 16.74 + 1 NM_006533 cercaexons first 291 413 10.38 + 0 NM_006533 cercaexons single 291 509 11.14 + 0 NM_006533 cercaexons internal 309 413 10.20 + 0 NM_006533 cercaexons terminal 309 509 10.96 + 0 NM_006533 cercaexons first 315 413 7.57 + 0 NM_006533 cercaexons single 315 509 8.32 + 0 NM_006533 cercaexons first 432 446 3.55 - 0 NM_006533 cercaexons first 432 459 2.00 - 0 NM_006533 cercaexons internal 432 521 4.93 - 2 NM_006533 cercaexons internal 432 521 4.12 - 0 NM_006533 cercaexons terminal 600 635 2.72 + 0 NM_006533 cercaexons internal 609 657 2.95 - 1 NM_006533 cercaexons internal 614 657 2.52 - 1 NM_006533 cercaexons terminal 737 792 3.08 + 1 NM_006533 cercaexons terminal 740 792 2.13 + 1 NM_006533 cercaexons first 789 830 4.25 + 0 NM_006533 cercaexons first 1140 1174 2.54 + 0 NM_006533 cercaexons single 1225 1311 2.78 + 0 NM_006533 cercaexons internal 1430 1467 4.75 - 2 NM_006533 cercaexons internal 1430 1467 3.78 - 1 NM_006533 cercaexons internal 1430 1467 3.38 - 0 NM_006533 cercaexons terminal 1497 1578 8.92 + 2 NM_006533 cercaexons internal 1497 1607 18.68 + 0 NM_006533 cercaexons internal 1497 1705 22.17 + 0 NM_006533 cercaexons terminal 1497 1787 18.57 + 0 NM_006533 cercaexons terminal 1503 1578 5.13 + 2 NM_006533 cercaexons internal 1503 1607 14.59 + 0 NM_006533 cercaexons internal 1503 1705 18.07 + 0 NM_006533 cercaexons terminal 1503 1787 14.48 + 0 NM_006533 cercaexons first 1617 1705 5.99 + 0 NM_006533 cercaexons single 1617 1787 2.40 + 0 NM_006533 cercaexons internal 1667 1720 3.78 - 1 NM_006533 cercaexons first 1674 1705 4.37 + 0 NM_006533 cercaexons terminal 1925 1948 7.23 + 0
El fitxer fasta d'aquesta seqüència és el següent: fitxer NM_005641
Primerament mostrem l'anotació real del gen. A continuació es mostren tots els exons que el programa cercaexons ha predit per la seqüència NM_005641. En color vermell es mostren els exons reals que el programa ha predit.
# anotacio del gen real NM_005641 chr7 Utr 1 477 . + . "H.sapiens" NM_005641 chr7 Utr 5089 5147 . + . "H.sapiens" NM_005641 chr7 First 5148 5303 . + . "H.sapiens" NM_005641 chr7 Internal 5403 5489 . + . "H.sapiens" NM_005641 chr7 Internal 5588 5741 . + . "H.sapiens" NM_005641 chr7 Internal 5952 6008 . + . "H.sapiens" NM_005641 chr7 Internal 6440 6559 . + . "H.sapiens" NM_005641 chr7 Internal 7104 7249 . + . "H.sapiens" NM_005641 chr7 Internal 7358 7435 . + . "H.sapiens" NM_005641 chr7 Internal 7528 7629 . + . "H.sapiens" NM_005641 chr7 Internal 8036 8218 . + . "H.sapiens" NM_005641 chr7 Internal 9083 9157 . + . "H.sapiens" NM_005641 chr7 Internal 9284 9409 . + . "H.sapiens" NM_005641 chr7 Internal 10817 10990 . + . "H.sapiens" NM_005641 chr7 Internal 11234 11431 . + . "H.sapiens" NM_005641 chr7 Terminal 11734 12111 . + . "H.sapiens" NM_005641 chr7 Utr 12112 12278 . + . "H.sapiens"
Per aquesta seqüència hem predit un total de 768 exons.
#Exons predits amb el CERCAEXONS per la sequencia NM_005641 NM_005641 cercaexons internal 82 113 4.25 - 2 NM_005641 cercaexons internal 82 149 5.66 - 2 NM_005641 cercaexons internal 82 149 4.88 - 0 NM_005641 cercaexons internal 82 179 4.51 - 2 NM_005641 cercaexons internal 82 260 5.62 - 0 NM_005641 cercaexons terminal 97 179 4.48 - 1 NM_005641 cercaexons internal 101 113 5.74 - 1 NM_005641 cercaexons internal 101 113 5.42 - 2 NM_005641 cercaexons internal 101 149 8.69 - 1 NM_005641 cercaexons internal 101 149 7.79 - 0 NM_005641 cercaexons internal 101 149 6.83 - 2 NM_005641 cercaexons internal 101 179 10.18 - 1 NM_005641 cercaexons internal 101 179 6.90 - 0 NM_005641 cercaexons internal 101 179 5.68 - 2 NM_005641 cercaexons internal 101 236 5.73 - 0 NM_005641 cercaexons internal 101 260 8.53 - 0 NM_005641 cercaexons internal 143 179 5.44 - 1 NM_005641 cercaexons internal 143 260 5.39 - 0 NM_005641 cercaexons internal 229 260 4.92 - 0 NM_005641 cercaexons terminal 596 636 4.30 + 1 NM_005641 cercaexons internal 596 839 6.93 + 0 NM_005641 cercaexons first 653 839 4.90 + 0 NM_005641 cercaexons internal 704 839 6.45 + 0 NM_005641 cercaexons internal 708 839 7.36 + 1 NM_005641 cercaexons internal 728 809 4.38 + 0 NM_005641 cercaexons internal 728 839 7.79 + 0 NM_005641 cercaexons internal 764 839 4.46 + 0 NM_005641 cercaexons internal 867 961 11.21 + 0 NM_005641 cercaexons internal 867 961 8.76 + 2 NM_005641 cercaexons terminal 867 965 6.53 + 0 NM_005641 cercaexons internal 867 1148 10.47 + 2 NM_005641 cercaexons internal 867 1199 7.26 + 2 NM_005641 cercaexons internal 867 1223 10.02 + 2 NM_005641 cercaexons terminal 867 1227 6.28 + 2 NM_005641 cercaexons first 883 961 5.35 + 0 NM_005641 cercaexons first 883 1148 7.06 + 0 NM_005641 cercaexons first 883 1223 6.61 + 0 NM_005641 cercaexons internal 894 961 6.80 + 0 NM_005641 cercaexons internal 894 961 5.67 + 1 NM_005641 cercaexons internal 894 961 4.95 + 2 NM_005641 cercaexons internal 894 1148 6.66 + 2 NM_005641 cercaexons internal 894 1223 6.21 + 2 NM_005641 cercaexons internal 946 961 4.97 + 1 NM_005641 cercaexons internal 946 961 4.15 + 2 NM_005641 cercaexons internal 946 961 4.05 + 0 NM_005641 cercaexons internal 946 1148 5.76 + 0 NM_005641 cercaexons internal 946 1223 5.31 + 0 NM_005641 cercaexons internal 1009 1148 7.78 + 0 NM_005641 cercaexons internal 1009 1148 4.44 + 1 NM_005641 cercaexons internal 1009 1199 4.57 + 0 NM_005641 cercaexons internal 1009 1223 7.33 + 0 NM_005641 cercaexons internal 1033 1148 5.01 + 0 NM_005641 cercaexons internal 1033 1148 4.40 + 1 NM_005641 cercaexons internal 1033 1223 4.56 + 0 NM_005641 cercaexons internal 1199 1352 4.06 + 0 NM_005641 cercaexons internal 1203 1225 5.09 - 1 NM_005641 cercaexons internal 1203 1225 4.72 - 0 NM_005641 cercaexons internal 1224 1352 6.53 + 1 NM_005641 cercaexons internal 1257 1352 7.35 + 2 NM_005641 cercaexons internal 1257 1352 5.13 + 1 NM_005641 cercaexons internal 1257 1439 6.77 + 2 NM_005641 cercaexons internal 1257 1500 4.89 + 2 NM_005641 cercaexons internal 1257 1532 7.40 + 2 NM_005641 cercaexons terminal 1257 1533 6.19 + 2 NM_005641 cercaexons internal 1315 1352 7.49 + 0 NM_005641 cercaexons internal 1315 1352 7.15 + 2 NM_005641 cercaexons terminal 1315 1427 4.77 + 1 NM_005641 cercaexons internal 1315 1439 6.92 + 0 NM_005641 cercaexons internal 1315 1500 5.04 + 0 NM_005641 cercaexons internal 1315 1532 7.54 + 0 NM_005641 cercaexons terminal 1315 1533 6.34 + 0 NM_005641 cercaexons internal 1319 1352 6.52 + 1 NM_005641 cercaexons internal 1319 1352 6.31 + 0 NM_005641 cercaexons terminal 1319 1427 4.95 + 2 NM_005641 cercaexons internal 1319 1439 5.95 + 1 NM_005641 cercaexons internal 1319 1500 4.07 + 1 NM_005641 cercaexons internal 1319 1532 6.57 + 1 NM_005641 cercaexons terminal 1319 1533 5.37 + 1 NM_005641 cercaexons internal 1339 1352 4.13 + 0 NM_005641 cercaexons internal 1339 1532 4.18 + 0 NM_005641 cercaexons first 1728 1836 8.22 + 0 NM_005641 cercaexons single 1728 1853 5.03 + 0 NM_005641 cercaexons first 1731 1836 7.88 + 0 NM_005641 cercaexons single 1731 1853 4.69 + 0 NM_005641 cercaexons internal 1740 1836 6.87 + 0 NM_005641 cercaexons internal 1740 1854 5.07 + 2 NM_005641 cercaexons internal 1820 1854 4.53 + 1 NM_005641 cercaexons internal 1844 1888 5.87 - 0 NM_005641 cercaexons internal 2040 2105 5.90 + 2 NM_005641 cercaexons internal 2040 2236 7.16 + 2 NM_005641 cercaexons internal 2040 2327 5.23 + 2 NM_005641 cercaexons first 2103 2236 6.67 + 0 NM_005641 cercaexons internal 2139 2236 6.41 + 0 NM_005641 cercaexons internal 2139 2236 5.22 + 1 NM_005641 cercaexons first 2524 2585 4.52 + 0 NM_005641 cercaexons first 2588 2644 6.25 + 0 NM_005641 cercaexons internal 2637 2677 6.13 - 0 NM_005641 cercaexons internal 2745 2795 4.31 + 2 NM_005641 cercaexons internal 2745 2801 5.03 + 2 NM_005641 cercaexons terminal 2745 2805 5.44 + 2 NM_005641 cercaexons internal 2883 2936 4.26 + 1 NM_005641 cercaexons internal 2883 2980 4.79 + 1 NM_005641 cercaexons internal 2883 3029 5.60 + 1 NM_005641 cercaexons internal 2883 3032 7.57 + 1 NM_005641 cercaexons terminal 2883 3106 7.19 + 1 NM_005641 cercaexons internal 2886 3029 4.59 + 1 NM_005641 cercaexons internal 2886 3032 6.55 + 1 NM_005641 cercaexons terminal 2886 3106 6.17 + 1 NM_005641 cercaexons internal 2965 3032 4.59 + 2 NM_005641 cercaexons terminal 2965 3106 4.21 + 2 NM_005641 cercaexons first 3315 3356 5.71 + 0 NM_005641 cercaexons first 3315 3369 4.28 + 0 NM_005641 cercaexons first 3318 3356 5.37 + 0 NM_005641 cercaexons internal 3414 3469 4.63 + 0 NM_005641 cercaexons internal 3414 3469 4.18 + 2 NM_005641 cercaexons internal 3414 3511 5.65 + 2 NM_005641 cercaexons internal 3464 3511 5.00 + 1 NM_005641 cercaexons internal 3488 3511 4.67 + 2 NM_005641 cercaexons internal 3551 3644 5.52 + 2 NM_005641 cercaexons internal 3554 3644 5.85 + 2 NM_005641 cercaexons internal 3559 3667 5.57 - 1 NM_005641 cercaexons internal 3584 3662 4.38 - 0 NM_005641 cercaexons internal 3584 3667 9.75 - 1 NM_005641 cercaexons internal 3701 3778 5.35 + 1 NM_005641 cercaexons internal 3701 3778 4.72 + 2 NM_005641 cercaexons internal 3701 3804 5.26 + 1 NM_005641 cercaexons internal 3705 3778 5.82 + 2 NM_005641 cercaexons internal 3705 3778 4.15 + 0 NM_005641 cercaexons internal 3705 3804 5.73 + 2 NM_005641 cercaexons terminal 4512 4608 4.32 + 2 NM_005641 cercaexons terminal 4512 4616 5.47 + 0 NM_005641 cercaexons terminal 4595 4626 6.87 - 1 NM_005641 cercaexons internal 4608 4626 8.70 - 1 NM_005641 cercaexons internal 4608 4626 6.24 - 2 NM_005641 cercaexons internal 4651 4720 8.30 + 1 NM_005641 cercaexons internal 4651 4720 5.69 + 0 NM_005641 cercaexons terminal 4651 4724 7.01 + 1 NM_005641 cercaexons internal 4651 4762 4.46 + 0 NM_005641 cercaexons internal 4751 4762 4.23 + 0 NM_005641 cercaexons internal 4802 4855 5.04 + 1 NM_005641 cercaexons internal 4802 4888 5.56 + 1 NM_005641 cercaexons internal 4802 4902 4.65 + 1 NM_005641 cercaexons internal 4805 4855 4.10 + 1 NM_005641 cercaexons internal 4805 4888 4.61 + 1 NM_005641 cercaexons internal 4884 4970 6.69 + 0 NM_005641 cercaexons internal 4884 5207 16.34 + 0 NM_005641 cercaexons internal 4884 5210 15.73 + 0 NM_005641 cercaexons internal 4884 5273 26.30 + 0 NM_005641 cercaexons internal 4884 5303 29.13 + 0 NM_005641 cercaexons terminal 4884 5378 25.86 + 0 NM_005641 cercaexons internal 4901 5207 13.39 + 2 NM_005641 cercaexons internal 4901 5210 12.78 + 2 NM_005641 cercaexons internal 4901 5273 23.34 + 2 NM_005641 cercaexons internal 4901 5303 26.18 + 2 NM_005641 cercaexons terminal 4901 5378 22.90 + 2 NM_005641 cercaexons internal 4927 5207 10.56 + 1 NM_005641 cercaexons internal 4927 5210 9.95 + 1 NM_005641 cercaexons internal 4927 5273 20.51 + 1 NM_005641 cercaexons internal 4927 5303 23.35 + 1 NM_005641 cercaexons terminal 4927 5378 20.07 + 1 NM_005641 cercaexons internal 4932 5207 10.11 + 0 NM_005641 cercaexons internal 4932 5210 9.50 + 0 NM_005641 cercaexons internal 4932 5273 20.07 + 0 NM_005641 cercaexons internal 4932 5303 22.90 + 0 NM_005641 cercaexons terminal 4932 5378 19.63 + 0 NM_005641 cercaexons internal 4960 4970 6.20 + 1 NM_005641 cercaexons internal 4960 4970 5.99 + 2 NM_005641 cercaexons terminal 4960 5155 7.86 + 2 NM_005641 cercaexons internal 4960 5207 15.85 + 1 NM_005641 cercaexons internal 4960 5210 15.24 + 1 NM_005641 cercaexons internal 4960 5273 25.80 + 1 NM_005641 cercaexons internal 4960 5303 28.63 + 1 NM_005641 cercaexons terminal 4960 5378 25.36 + 1 NM_005641 cercaexons first 4977 5207 10.10 + 0 NM_005641 cercaexons first 4977 5210 9.49 + 0 NM_005641 cercaexons first 4977 5273 20.06 + 0 NM_005641 cercaexons first 4977 5303 22.89 + 0 NM_005641 cercaexons single 4977 5378 19.62 + 0 NM_005641 cercaexons internal 4980 4994 6.14 - 2 NM_005641 cercaexons internal 4980 4994 5.76 - 1 NM_005641 cercaexons internal 4980 4994 5.01 - 0 NM_005641 cercaexons internal 4980 5248 6.52 - 1 NM_005641 cercaexons terminal 5089 5155 6.21 + 2 NM_005641 cercaexons internal 5089 5207 13.07 + 1 NM_005641 cercaexons internal 5089 5210 12.46 + 1 NM_005641 cercaexons internal 5089 5273 23.03 + 1 NM_005641 cercaexons internal 5089 5303 25.86 + 1 NM_005641 cercaexons terminal 5089 5378 22.59 + 1 NM_005641 cercaexons terminal 5126 5155 4.30 + 0 NM_005641 cercaexons internal 5126 5207 13.60 + 2 NM_005641 cercaexons internal 5126 5210 12.99 + 2 NM_005641 cercaexons internal 5126 5273 23.56 + 2 NM_005641 cercaexons internal 5126 5303 26.39 + 2 NM_005641 cercaexons terminal 5126 5378 23.12 + 2 NM_005641 cercaexons first 5148 5207 8.40 + 0 NM_005641 cercaexons first 5148 5210 7.79 + 0 NM_005641 cercaexons first 5148 5273 18.36 + 0 NM_005641 cercaexons first 5148 5303 21.19 + 0 NM_005641 cercaexons single 5148 5378 17.92 + 0 NM_005641 cercaexons first 5202 5273 11.14 + 0 NM_005641 cercaexons first 5202 5303 13.98 + 0 NM_005641 cercaexons single 5202 5378 10.70 + 0 NM_005641 cercaexons first 5223 5273 7.90 + 0 NM_005641 cercaexons first 5223 5303 10.73 + 0 NM_005641 cercaexons single 5223 5378 7.46 + 0 NM_005641 cercaexons internal 5238 5303 6.77 + 0 NM_005641 cercaexons internal 5403 5489 11.26 + 0 NM_005641 cercaexons internal 5403 5517 12.37 + 0 NM_005641 cercaexons internal 5403 5639 13.26 + 0 NM_005641 cercaexons internal 5403 5677 10.30 + 0 NM_005641 cercaexons terminal 5403 5687 10.28 + 0 NM_005641 cercaexons first 5418 5489 7.68 + 0 NM_005641 cercaexons first 5418 5517 8.79 + 0 NM_005641 cercaexons first 5418 5639 9.68 + 0 NM_005641 cercaexons first 5418 5677 6.72 + 0 NM_005641 cercaexons single 5418 5687 6.70 + 0 NM_005641 cercaexons first 5424 5489 7.45 + 0 NM_005641 cercaexons first 5424 5517 8.56 + 0 NM_005641 cercaexons first 5424 5639 9.46 + 0 NM_005641 cercaexons first 5424 5677 6.49 + 0 NM_005641 cercaexons single 5424 5687 6.47 + 0 NM_005641 cercaexons internal 5537 5639 6.84 + 0 NM_005641 cercaexons internal 5537 5677 7.26 + 0 NM_005641 cercaexons internal 5537 5716 10.90 + 0 NM_005641 cercaexons internal 5537 5741 16.08 + 0 NM_005641 cercaexons internal 5537 5762 15.40 + 0 NM_005641 cercaexons internal 5537 5792 20.78 + 0 NM_005641 cercaexons internal 5537 5796 19.65 + 0 NM_005641 cercaexons terminal 5537 5800 18.25 + 0 NM_005641 cercaexons internal 5541 5639 8.51 + 1 NM_005641 cercaexons internal 5541 5677 8.94 + 1 NM_005641 cercaexons internal 5541 5716 12.58 + 1 NM_005641 cercaexons internal 5541 5741 17.76 + 1 NM_005641 cercaexons internal 5541 5762 17.08 + 1 NM_005641 cercaexons internal 5541 5792 22.46 + 1 NM_005641 cercaexons internal 5541 5796 21.33 + 1 NM_005641 cercaexons terminal 5541 5800 19.93 + 1 NM_005641 cercaexons internal 5588 5639 10.53 + 0 NM_005641 cercaexons internal 5588 5639 5.55 + 2 NM_005641 cercaexons internal 5588 5677 10.95 + 0 NM_005641 cercaexons internal 5588 5716 14.59 + 0 NM_005641 cercaexons internal 5588 5741 19.77 + 0 NM_005641 cercaexons internal 5588 5762 19.09 + 0 NM_005641 cercaexons internal 5588 5792 24.47 + 0 NM_005641 cercaexons internal 5588 5796 23.34 + 0 NM_005641 cercaexons terminal 5588 5800 21.94 + 0 NM_005641 cercaexons internal 5612 5639 5.80 + 0 NM_005641 cercaexons internal 5612 5677 6.22 + 0 NM_005641 cercaexons internal 5612 5716 9.86 + 0 NM_005641 cercaexons internal 5612 5741 15.04 + 0 NM_005641 cercaexons internal 5612 5762 14.36 + 0 NM_005641 cercaexons internal 5612 5792 19.74 + 0 NM_005641 cercaexons internal 5612 5796 18.61 + 0 NM_005641 cercaexons terminal 5612 5800 17.21 + 0 NM_005641 cercaexons internal 5742 5792 5.43 + 1 NM_005641 cercaexons internal 5742 5796 4.31 + 1 NM_005641 cercaexons internal 5742 5847 9.04 + 2 NM_005641 cercaexons internal 5742 5847 8.58 + 0 NM_005641 cercaexons terminal 5742 5933 6.09 + 0 NM_005641 cercaexons first 5791 5847 5.63 + 0 NM_005641 cercaexons internal 5873 5931 6.54 - 0 NM_005641 cercaexons first 5891 6008 7.65 + 0 NM_005641 cercaexons single 5891 6076 5.34 + 0 NM_005641 cercaexons internal 5920 5931 5.78 - 1 NM_005641 cercaexons internal 5920 5931 4.01 - 0 NM_005641 cercaexons internal 5952 6008 11.70 + 1 NM_005641 cercaexons internal 5952 6008 8.56 + 2 NM_005641 cercaexons terminal 5952 6076 9.38 + 1 NM_005641 cercaexons terminal 6009 6200 5.56 + 0 NM_005641 cercaexons single 6018 6200 5.70 + 0 NM_005641 cercaexons terminal 6078 6204 4.93 + 2 NM_005641 cercaexons terminal 6082 6200 4.62 + 1 NM_005641 cercaexons terminal 6082 6204 5.77 + 0 NM_005641 cercaexons internal 6082 6246 4.20 + 2 NM_005641 cercaexons internal 6339 6482 9.99 + 2 NM_005641 cercaexons internal 6339 6529 18.74 + 2 NM_005641 cercaexons internal 6339 6559 24.38 + 2 NM_005641 cercaexons terminal 6339 6573 19.21 + 2 NM_005641 cercaexons internal 6353 6482 7.38 + 1 NM_005641 cercaexons internal 6353 6529 16.14 + 1 NM_005641 cercaexons internal 6353 6559 21.78 + 1 NM_005641 cercaexons terminal 6353 6573 16.61 + 1 NM_005641 cercaexons first 6355 6482 9.35 + 0 NM_005641 cercaexons first 6355 6529 18.10 + 0 NM_005641 cercaexons first 6355 6559 23.74 + 0 NM_005641 cercaexons single 6355 6573 18.58 + 0 NM_005641 cercaexons first 6400 6482 8.77 + 0 NM_005641 cercaexons first 6400 6529 17.53 + 0 NM_005641 cercaexons first 6400 6559 23.17 + 0 NM_005641 cercaexons single 6400 6573 18.00 + 0 NM_005641 cercaexons terminal 6440 6481 5.94 + 0 NM_005641 cercaexons internal 6440 6482 8.74 + 1 NM_005641 cercaexons internal 6440 6529 17.50 + 1 NM_005641 cercaexons internal 6440 6559 23.14 + 1 NM_005641 cercaexons terminal 6440 6573 17.97 + 1 NM_005641 cercaexons internal 6449 6529 11.68 + 1 NM_005641 cercaexons internal 6449 6559 17.32 + 1 NM_005641 cercaexons terminal 6449 6573 12.15 + 1 NM_005641 cercaexons internal 6646 6683 5.11 + 2 NM_005641 cercaexons internal 6650 6683 4.32 + 0 NM_005641 cercaexons internal 6670 6683 4.97 + 2 NM_005641 cercaexons internal 6719 6767 4.81 + 1 NM_005641 cercaexons internal 6811 6904 4.51 + 2 NM_005641 cercaexons internal 6814 6904 5.67 + 0 NM_005641 cercaexons internal 6814 6904 5.21 + 2 NM_005641 cercaexons internal 6840 6904 4.33 + 1 NM_005641 cercaexons internal 6891 6955 5.81 + 0 NM_005641 cercaexons internal 6891 7032 5.74 + 0 NM_005641 cercaexons terminal 6891 7037 4.69 + 0 NM_005641 cercaexons internal 6905 6955 4.53 + 2 NM_005641 cercaexons internal 6905 7032 4.46 + 2 NM_005641 cercaexons first 6917 6955 5.67 + 0 NM_005641 cercaexons first 6917 7032 7.80 + 0 NM_005641 cercaexons single 6917 7054 6.87 + 0 NM_005641 cercaexons first 6930 6955 4.00 + 0 NM_005641 cercaexons internal 6935 7080 4.45 - 2 NM_005641 cercaexons internal 6950 7080 4.54 - 2 NM_005641 cercaexons internal 6983 7080 5.70 - 0 NM_005641 cercaexons internal 6983 7080 4.39 - 2 NM_005641 cercaexons internal 7021 7249 6.30 + 0 NM_005641 cercaexons terminal 7021 7281 4.26 + 0 NM_005641 cercaexons internal 7029 7080 4.85 - 0 NM_005641 cercaexons internal 7082 7222 11.57 + 0 NM_005641 cercaexons internal 7082 7249 18.30 + 0 NM_005641 cercaexons internal 7082 7249 4.95 + 1 NM_005641 cercaexons terminal 7082 7336 18.47 + 0 NM_005641 cercaexons internal 7104 7222 12.26 + 1 NM_005641 cercaexons internal 7104 7249 18.99 + 1 NM_005641 cercaexons internal 7104 7249 6.09 + 2 NM_005641 cercaexons terminal 7104 7281 4.06 + 2 NM_005641 cercaexons terminal 7104 7336 19.16 + 1 NM_005641 cercaexons internal 7226 7334 4.73 - 1 NM_005641 cercaexons internal 7358 7435 13.63 + 0 NM_005641 cercaexons internal 7358 7435 8.32 + 1 NM_005641 cercaexons internal 7358 7435 6.98 + 2 NM_005641 cercaexons terminal 7358 7447 10.07 + 0 NM_005641 cercaexons internal 7358 7482 8.26 + 1 NM_005641 cercaexons internal 7358 7527 12.59 + 1 NM_005641 cercaexons internal 7358 7539 10.18 + 1 NM_005641 cercaexons internal 7358 7579 14.18 + 1 NM_005641 cercaexons internal 7358 7584 15.19 + 1 NM_005641 cercaexons internal 7358 7629 18.57 + 1 NM_005641 cercaexons internal 7358 7690 20.68 + 1 NM_005641 cercaexons internal 7358 7697 21.12 + 1 NM_005641 cercaexons terminal 7358 7719 19.82 + 1 NM_005641 cercaexons internal 7433 7527 4.96 + 1 NM_005641 cercaexons internal 7433 7579 6.55 + 1 NM_005641 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+ 1 NM_005641 cercaexons internal 11748 12272 6.75 + 0 NM_005641 cercaexons internal 11768 11891 5.79 + 1 NM_005641 cercaexons terminal 11768 12111 15.25 + 1 NM_005641 cercaexons internal 11768 12153 6.36 + 2 NM_005641 cercaexons internal 11768 12153 4.21 + 0 NM_005641 cercaexons internal 11768 12167 7.13 + 2 NM_005641 cercaexons internal 11768 12167 4.32 + 0 NM_005641 cercaexons internal 11768 12272 5.02 + 2 NM_005641 cercaexons internal 11808 11891 5.08 + 2 NM_005641 cercaexons terminal 11808 12111 14.54 + 2 NM_005641 cercaexons internal 11808 12153 7.59 + 1 NM_005641 cercaexons internal 11808 12153 7.25 + 0 NM_005641 cercaexons internal 11808 12167 8.02 + 0 NM_005641 cercaexons internal 11808 12167 7.70 + 1 NM_005641 cercaexons terminal 11808 12190 4.45 + 1 NM_005641 cercaexons internal 11808 12272 5.91 + 0 NM_005641 cercaexons terminal 11815 12111 11.60 + 0 NM_005641 cercaexons internal 11815 12153 6.18 + 1 NM_005641 cercaexons internal 11815 12153 5.95 + 2 NM_005641 cercaexons internal 11815 12167 6.95 + 1 NM_005641 cercaexons internal 11815 12167 6.07 + 2 NM_005641 cercaexons internal 11815 12272 4.84 + 1 NM_005641 cercaexons terminal 11859 12111 9.91 + 2 NM_005641 cercaexons internal 11859 12153 10.89 + 1 NM_005641 cercaexons internal 11859 12153 9.81 + 0 NM_005641 cercaexons internal 11859 12167 11.01 + 1 NM_005641 cercaexons internal 11859 12167 10.59 + 0 NM_005641 cercaexons terminal 11859 12190 7.75 + 1 NM_005641 cercaexons internal 11859 12272 8.47 + 0 NM_005641 cercaexons terminal 11921 12111 11.22 + 1 NM_005641 cercaexons internal 11921 12153 14.22 + 2 NM_005641 cercaexons internal 11921 12153 11.55 + 0 NM_005641 cercaexons internal 11921 12167 14.99 + 2 NM_005641 cercaexons internal 11921 12167 11.66 + 0 NM_005641 cercaexons terminal 11921 12190 8.41 + 0 NM_005641 cercaexons internal 11921 12272 12.88 + 2 NM_005641 cercaexons terminal 11960 12111 8.73 + 1 NM_005641 cercaexons internal 11960 12153 13.98 + 2 NM_005641 cercaexons internal 11960 12153 7.76 + 0 NM_005641 cercaexons internal 11960 12167 14.76 + 2 NM_005641 cercaexons internal 11960 12167 7.87 + 0 NM_005641 cercaexons terminal 11960 12190 4.62 + 0 NM_005641 cercaexons internal 11960 12272 12.64 + 2 NM_005641 cercaexons terminal 11994 12111 8.57 + 2 NM_005641 cercaexons internal 11994 12153 9.67 + 0 NM_005641 cercaexons internal 11994 12153 8.85 + 1 NM_005641 cercaexons internal 11994 12167 10.45 + 0 NM_005641 cercaexons internal 11994 12167 8.96 + 1 NM_005641 cercaexons terminal 11994 12190 5.71 + 1 NM_005641 cercaexons internal 11994 12272 8.33 + 0 NM_005641 cercaexons terminal 12000 12111 4.18 + 2 NM_005641 cercaexons internal 12000 12153 4.99 + 0 NM_005641 cercaexons internal 12000 12167 5.76 + 0 NM_005641 cercaexons internal 12000 12167 4.02 + 1 NM_005641 cercaexons internal 12047 12153 4.64 + 2 NM_005641 cercaexons internal 12047 12167 5.42 + 2 NM_005641 cercaexons terminal 12053 12111 5.51 + 1 NM_005641 cercaexons internal 12053 12153 7.77 + 2 NM_005641 cercaexons internal 12053 12153 5.14 + 0 NM_005641 cercaexons internal 12053 12167 8.55 + 2 NM_005641 cercaexons internal 12053 12167 5.25 + 0 NM_005641 cercaexons internal 12053 12272 6.44 + 2 NM_005641 cercaexons internal 12100 12153 4.58 + 1 NM_005641 cercaexons internal 12100 12167 5.36 + 1
Hem pogut observar que el programa funciona correctament i que prediu tots els exons de les dues seqüències exemple. Un dels llocs de splicing de la seqüència NM_005641 tenia una puntuació molt dolenta i ens ha obligat a baixar el llindar fins a -2. Per trobar tots els exons d'un gen hem de jugar amb els diferents llindars fins a trobar-los tots, encara que és curiós veure com la màquinaria de splicing tria uns llocs de splicing en teoria pitjors tenint-ne d'altres millors, això ens indica que encara falta refinar molt els sistemes de predicció d'exons.
Podríem pensar que les matrius de pesos utilitzades per a la realització del programa són prou correctes ja que han estat obtingudes a base de comparar molts llocs de splicing donadors i acceptors. No obstant, si enlloc d'utilitzar aquestes matrius utilitzéssim unes matrius de pesos condicionades, les prediccions serien més acurades. Aquest model s'anomena Model de Markov i les matrius utilitzades a l'hora de puntuar cada nucleòtid del possible lloc de splicing, tenen en compte les posicions anteriors. Hi ha diferents ordres; per exemple un Model de Markov d'ordre 2, a l'hora de puntuar una posició té en compte els nucleòtids de les dues posicions anteriors.
El número d'exons predits sembla massa alt, però no ho és tant si pensem que estem predint els exons pels dos "strands" possibles, i que estem predint exons de tots els tipus (single, first, internal, terminal). A més a més si ensemblem aquests resultats per formar un gen, obtenim uns resultats prou satisfactoris. Per exemple podem utilitzar el servidor geneid del GRIB (Grup de Recerca en Informàtica Biomèdica de Barcelona).
Per obtenir una representació gràfica dels resultats obtinguts podeu utilitzar el programa gff2ps