Resultats i conclusions

Per tal de comprovar el funcionament del programa l'hem executat per a dues seqüències de les quals en coneixem l'anotació real dels exons. Observant els resultats comprovem que el programa prediu tots els exons d'ambdues seqüències.

1. Seqüència NM_006533

El fitxer fasta d'aquesta seqüència és el següent: fitxer NM_006533

Primerament mostrem l'anotació real del gen. A continuació es mostren tots els exons que el programa cercaexons ha predit per la seqüència NM_006533. En color vermell es mostren els exons reals que el programa ha predit. 

# annotacio del gen real
NM_006533	chr19	Utr       	1	70	.	+	.	"H.sapiens"
NM_006533	chr19	First	        71	197	.	+	.	"H.sapiens"
NM_006533	chr19	Internal	280	413	.	+	        "H.sapiens"
NM_006533	chr19	Internal	1497	1607	.	+	        "H.sapiens"
NM_006533	chr19	Terminal	1925	1948	.	+	        "H.sapiens"
NM_006533	chr19	Utr     	1949	2019	.	+	.	"H.sapiens"

Selecció dels llindars
  • llindar de puntuació màxima pels senyals de splicing: 1
  • llindar de puntuació màxima pels exons predits: 2

    Per aquesta seqüència hem predit un total de 58 exons. 

    #Exons predits amb el CERCAEXONS per la sequencia NM_006533

NM_006533	cercaexons	internal	65	85	5.96	+	0
NM_006533	cercaexons	internal	65	85	5.09	+	1
NM_006533	cercaexons	internal	65	85	4.53	+	2
NM_006533	cercaexons	terminal	65	165	2.09	+	1
NM_006533	cercaexons	internal	65	197	13.55	+	0
NM_006533	cercaexons	internal	65	197	6.99	+	2
NM_006533	cercaexons	internal	65	413	16.60	+	2
NM_006533	cercaexons	internal	65	413	12.25	+	0
NM_006533	cercaexons	terminal	65	460	10.48	+	0
NM_006533	cercaexons	terminal	65	509	17.35	+	2
NM_006533	cercaexons	first   	71	85	2.63	+	0
NM_006533	cercaexons	first   	71	197	10.22	+	0
NM_006533	cercaexons	first   	71	413	8.92	+	0
NM_006533	cercaexons	single  	71	460	7.15	+	0
NM_006533	cercaexons	first   	149	197	6.68	+	0
NM_006533	cercaexons	first   	149	413	5.38	+	0
NM_006533	cercaexons	single  	149	460	3.61	+	0
NM_006533	cercaexons	first   	204	413	12.05	+	0
NM_006533	cercaexons	single  	204	509	12.80	+	0
NM_006533	cercaexons	internal	246	278	5.86	-	1
NM_006533	cercaexons	terminal	280	339	3.77	+	0
NM_006533	cercaexons	internal	280	413	15.99	+	1
NM_006533	cercaexons	internal	280	413	2.93	+	2
NM_006533	cercaexons	terminal	280	509	16.74	+	1
NM_006533	cercaexons	first   	291	413	10.38	+	0
NM_006533	cercaexons	single  	291	509	11.14	+	0
NM_006533	cercaexons	internal	309	413	10.20	+	0
NM_006533	cercaexons	terminal	309	509	10.96	+	0
NM_006533	cercaexons	first   	315	413	7.57	+	0
NM_006533	cercaexons	single  	315	509	8.32	+	0
NM_006533	cercaexons	first   	432	446	3.55	-	0
NM_006533	cercaexons	first   	432	459	2.00	-	0
NM_006533	cercaexons	internal	432	521	4.93	-	2
NM_006533	cercaexons	internal	432	521	4.12	-	0
NM_006533	cercaexons	terminal	600	635	2.72	+	0
NM_006533	cercaexons	internal	609	657	2.95	-	1
NM_006533	cercaexons	internal	614	657	2.52	-	1
NM_006533	cercaexons	terminal	737	792	3.08	+	1
NM_006533	cercaexons	terminal	740	792	2.13	+	1
NM_006533	cercaexons	first   	789	830	4.25	+	0
NM_006533	cercaexons	first   	1140	1174	2.54	+	0
NM_006533	cercaexons	single  	1225	1311	2.78	+	0
NM_006533	cercaexons	internal	1430	1467	4.75	-	2
NM_006533	cercaexons	internal	1430	1467	3.78	-	1
NM_006533	cercaexons	internal	1430	1467	3.38	-	0
NM_006533	cercaexons	terminal	1497	1578	8.92	+	2
NM_006533	cercaexons	internal	1497	1607	18.68	+	0
NM_006533	cercaexons	internal	1497	1705	22.17	+	0
NM_006533	cercaexons	terminal	1497	1787	18.57	+	0
NM_006533	cercaexons	terminal	1503	1578	5.13	+	2
NM_006533	cercaexons	internal	1503	1607	14.59	+	0
NM_006533	cercaexons	internal	1503	1705	18.07	+	0
NM_006533	cercaexons	terminal	1503	1787	14.48	+	0
NM_006533	cercaexons	first   	1617	1705	5.99	+	0
NM_006533	cercaexons	single  	1617	1787	2.40	+	0
NM_006533	cercaexons	internal	1667	1720	3.78	-	1
NM_006533	cercaexons	first   	1674	1705	4.37	+	0
NM_006533	cercaexons	terminal	1925	1948	7.23	+	0

    2. Seqüència NM_005641

    El fitxer fasta d'aquesta seqüència és el següent: fitxer NM_005641

    Primerament mostrem l'anotació real del gen. A continuació es mostren tots els exons que el programa cercaexons ha predit per la seqüència NM_005641. En color vermell es mostren els exons reals que el programa ha predit. 

    # anotacio del gen real
NM_005641	chr7	Utr	1	477	.	+	.	"H.sapiens"
NM_005641	chr7	Utr	5089	5147	.	+	.	"H.sapiens"
NM_005641	chr7	First	5148	5303	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	5403	5489	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	5588	5741	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	5952	6008	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	6440	6559	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	7104	7249	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	7358	7435	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	7528	7629	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	8036	8218	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	9083	9157	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	9284	9409	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	10817	10990	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	11234	11431	.	+	.	"H.sapiens"
NM_005641	chr7	Terminal	11734	12111	.	+	.	"H.sapiens"
NM_005641	chr7	Utr	12112	12278	.	+	.	"H.sapiens"

    Selecció dels llindars

    Per aquesta seqüència hem predit un total de 768 exons. 

    #Exons predits amb el CERCAEXONS per la sequencia NM_005641

NM_005641	cercaexons	internal	82	113	4.25	-	2
NM_005641	cercaexons	internal	82	149	5.66	-	2
NM_005641	cercaexons	internal	82	149	4.88	-	0
NM_005641	cercaexons	internal	82	179	4.51	-	2
NM_005641	cercaexons	internal	82	260	5.62	-	0
NM_005641	cercaexons	terminal	97	179	4.48	-	1
NM_005641	cercaexons	internal	101	113	5.74	-	1
NM_005641	cercaexons	internal	101	113	5.42	-	2
NM_005641	cercaexons	internal	101	149	8.69	-	1
NM_005641	cercaexons	internal	101	149	7.79	-	0
NM_005641	cercaexons	internal	101	149	6.83	-	2
NM_005641	cercaexons	internal	101	179	10.18	-	1
NM_005641	cercaexons	internal	101	179	6.90	-	0
NM_005641	cercaexons	internal	101	179	5.68	-	2
NM_005641	cercaexons	internal	101	236	5.73	-	0
NM_005641	cercaexons	internal	101	260	8.53	-	0
NM_005641	cercaexons	internal	143	179	5.44	-	1
NM_005641	cercaexons	internal	143	260	5.39	-	0
NM_005641	cercaexons	internal	229	260	4.92	-	0
NM_005641	cercaexons	terminal	596	636	4.30	+	1
NM_005641	cercaexons	internal	596	839	6.93	+	0
NM_005641	cercaexons	first   	653	839	4.90	+	0
NM_005641	cercaexons	internal	704	839	6.45	+	0
NM_005641	cercaexons	internal	708	839	7.36	+	1
NM_005641	cercaexons	internal	728	809	4.38	+	0
NM_005641	cercaexons	internal	728	839	7.79	+	0
NM_005641	cercaexons	internal	764	839	4.46	+	0
NM_005641	cercaexons	internal	867	961	11.21	+	0
NM_005641	cercaexons	internal	867	961	8.76	+	2
NM_005641	cercaexons	terminal	867	965	6.53	+	0
NM_005641	cercaexons	internal	867	1148	10.47	+	2
NM_005641	cercaexons	internal	867	1199	7.26	+	2
NM_005641	cercaexons	internal	867	1223	10.02	+	2
NM_005641	cercaexons	terminal	867	1227	6.28	+	2
NM_005641	cercaexons	first   	883	961	5.35	+	0
NM_005641	cercaexons	first   	883	1148	7.06	+	0
NM_005641	cercaexons	first   	883	1223	6.61	+	0
NM_005641	cercaexons	internal	894	961	6.80	+	0
NM_005641	cercaexons	internal	894	961	5.67	+	1
NM_005641	cercaexons	internal	894	961	4.95	+	2
NM_005641	cercaexons	internal	894	1148	6.66	+	2
NM_005641	cercaexons	internal	894	1223	6.21	+	2
NM_005641	cercaexons	internal	946	961	4.97	+	1
NM_005641	cercaexons	internal	946	961	4.15	+	2
NM_005641	cercaexons	internal	946	961	4.05	+	0
NM_005641	cercaexons	internal	946	1148	5.76	+	0
NM_005641	cercaexons	internal	946	1223	5.31	+	0
NM_005641	cercaexons	internal	1009	1148	7.78	+	0
NM_005641	cercaexons	internal	1009	1148	4.44	+	1
NM_005641	cercaexons	internal	1009	1199	4.57	+	0
NM_005641	cercaexons	internal	1009	1223	7.33	+	0
NM_005641	cercaexons	internal	1033	1148	5.01	+	0
NM_005641	cercaexons	internal	1033	1148	4.40	+	1
NM_005641	cercaexons	internal	1033	1223	4.56	+	0
NM_005641	cercaexons	internal	1199	1352	4.06	+	0
NM_005641	cercaexons	internal	1203	1225	5.09	-	1
NM_005641	cercaexons	internal	1203	1225	4.72	-	0
NM_005641	cercaexons	internal	1224	1352	6.53	+	1
NM_005641	cercaexons	internal	1257	1352	7.35	+	2
NM_005641	cercaexons	internal	1257	1352	5.13	+	1
NM_005641	cercaexons	internal	1257	1439	6.77	+	2
NM_005641	cercaexons	internal	1257	1500	4.89	+	2
NM_005641	cercaexons	internal	1257	1532	7.40	+	2
NM_005641	cercaexons	terminal	1257	1533	6.19	+	2
NM_005641	cercaexons	internal	1315	1352	7.49	+	0
NM_005641	cercaexons	internal	1315	1352	7.15	+	2
NM_005641	cercaexons	terminal	1315	1427	4.77	+	1
NM_005641	cercaexons	internal	1315	1439	6.92	+	0
NM_005641	cercaexons	internal	1315	1500	5.04	+	0
NM_005641	cercaexons	internal	1315	1532	7.54	+	0
NM_005641	cercaexons	terminal	1315	1533	6.34	+	0
NM_005641	cercaexons	internal	1319	1352	6.52	+	1
NM_005641	cercaexons	internal	1319	1352	6.31	+	0
NM_005641	cercaexons	terminal	1319	1427	4.95	+	2
NM_005641	cercaexons	internal	1319	1439	5.95	+	1
NM_005641	cercaexons	internal	1319	1500	4.07	+	1
NM_005641	cercaexons	internal	1319	1532	6.57	+	1
NM_005641	cercaexons	terminal	1319	1533	5.37	+	1
NM_005641	cercaexons	internal	1339	1352	4.13	+	0
NM_005641	cercaexons	internal	1339	1532	4.18	+	0
NM_005641	cercaexons	first   	1728	1836	8.22	+	0
NM_005641	cercaexons	single  	1728	1853	5.03	+	0
NM_005641	cercaexons	first   	1731	1836	7.88	+	0
NM_005641	cercaexons	single  	1731	1853	4.69	+	0
NM_005641	cercaexons	internal	1740	1836	6.87	+	0
NM_005641	cercaexons	internal	1740	1854	5.07	+	2
NM_005641	cercaexons	internal	1820	1854	4.53	+	1
NM_005641	cercaexons	internal	1844	1888	5.87	-	0
NM_005641	cercaexons	internal	2040	2105	5.90	+	2
NM_005641	cercaexons	internal	2040	2236	7.16	+	2
NM_005641	cercaexons	internal	2040	2327	5.23	+	2
NM_005641	cercaexons	first   	2103	2236	6.67	+	0
NM_005641	cercaexons	internal	2139	2236	6.41	+	0
NM_005641	cercaexons	internal	2139	2236	5.22	+	1
NM_005641	cercaexons	first   	2524	2585	4.52	+	0
NM_005641	cercaexons	first   	2588	2644	6.25	+	0
NM_005641	cercaexons	internal	2637	2677	6.13	-	0
NM_005641	cercaexons	internal	2745	2795	4.31	+	2
NM_005641	cercaexons	internal	2745	2801	5.03	+	2
NM_005641	cercaexons	terminal	2745	2805	5.44	+	2
NM_005641	cercaexons	internal	2883	2936	4.26	+	1
NM_005641	cercaexons	internal	2883	2980	4.79	+	1
NM_005641	cercaexons	internal	2883	3029	5.60	+	1
NM_005641	cercaexons	internal	2883	3032	7.57	+	1
NM_005641	cercaexons	terminal	2883	3106	7.19	+	1
NM_005641	cercaexons	internal	2886	3029	4.59	+	1
NM_005641	cercaexons	internal	2886	3032	6.55	+	1
NM_005641	cercaexons	terminal	2886	3106	6.17	+	1
NM_005641	cercaexons	internal	2965	3032	4.59	+	2
NM_005641	cercaexons	terminal	2965	3106	4.21	+	2
NM_005641	cercaexons	first   	3315	3356	5.71	+	0
NM_005641	cercaexons	first   	3315	3369	4.28	+	0
NM_005641	cercaexons	first   	3318	3356	5.37	+	0
NM_005641	cercaexons	internal	3414	3469	4.63	+	0
NM_005641	cercaexons	internal	3414	3469	4.18	+	2
NM_005641	cercaexons	internal	3414	3511	5.65	+	2
NM_005641	cercaexons	internal	3464	3511	5.00	+	1
NM_005641	cercaexons	internal	3488	3511	4.67	+	2
NM_005641	cercaexons	internal	3551	3644	5.52	+	2
NM_005641	cercaexons	internal	3554	3644	5.85	+	2
NM_005641	cercaexons	internal	3559	3667	5.57	-	1
NM_005641	cercaexons	internal	3584	3662	4.38	-	0
NM_005641	cercaexons	internal	3584	3667	9.75	-	1
NM_005641	cercaexons	internal	3701	3778	5.35	+	1
NM_005641	cercaexons	internal	3701	3778	4.72	+	2
NM_005641	cercaexons	internal	3701	3804	5.26	+	1
NM_005641	cercaexons	internal	3705	3778	5.82	+	2
NM_005641	cercaexons	internal	3705	3778	4.15	+	0
NM_005641	cercaexons	internal	3705	3804	5.73	+	2
NM_005641	cercaexons	terminal	4512	4608	4.32	+	2
NM_005641	cercaexons	terminal	4512	4616	5.47	+	0
NM_005641	cercaexons	terminal	4595	4626	6.87	-	1
NM_005641	cercaexons	internal	4608	4626	8.70	-	1
NM_005641	cercaexons	internal	4608	4626	6.24	-	2
NM_005641	cercaexons	internal	4651	4720	8.30	+	1
NM_005641	cercaexons	internal	4651	4720	5.69	+	0
NM_005641	cercaexons	terminal	4651	4724	7.01	+	1
NM_005641	cercaexons	internal	4651	4762	4.46	+	0
NM_005641	cercaexons	internal	4751	4762	4.23	+	0
NM_005641	cercaexons	internal	4802	4855	5.04	+	1
NM_005641	cercaexons	internal	4802	4888	5.56	+	1
NM_005641	cercaexons	internal	4802	4902	4.65	+	1
NM_005641	cercaexons	internal	4805	4855	4.10	+	1
NM_005641	cercaexons	internal	4805	4888	4.61	+	1
NM_005641	cercaexons	internal	4884	4970	6.69	+	0
NM_005641	cercaexons	internal	4884	5207	16.34	+	0
NM_005641	cercaexons	internal	4884	5210	15.73	+	0
NM_005641	cercaexons	internal	4884	5273	26.30	+	0
NM_005641	cercaexons	internal	4884	5303	29.13	+	0
NM_005641	cercaexons	terminal	4884	5378	25.86	+	0
NM_005641	cercaexons	internal	4901	5207	13.39	+	2
NM_005641	cercaexons	internal	4901	5210	12.78	+	2
NM_005641	cercaexons	internal	4901	5273	23.34	+	2
NM_005641	cercaexons	internal	4901	5303	26.18	+	2
NM_005641	cercaexons	terminal	4901	5378	22.90	+	2
NM_005641	cercaexons	internal	4927	5207	10.56	+	1
NM_005641	cercaexons	internal	4927	5210	9.95	+	1
NM_005641	cercaexons	internal	4927	5273	20.51	+	1
NM_005641	cercaexons	internal	4927	5303	23.35	+	1
NM_005641	cercaexons	terminal	4927	5378	20.07	+	1
NM_005641	cercaexons	internal	4932	5207	10.11	+	0
NM_005641	cercaexons	internal	4932	5210	9.50	+	0
NM_005641	cercaexons	internal	4932	5273	20.07	+	0
NM_005641	cercaexons	internal	4932	5303	22.90	+	0
NM_005641	cercaexons	terminal	4932	5378	19.63	+	0
NM_005641	cercaexons	internal	4960	4970	6.20	+	1
NM_005641	cercaexons	internal	4960	4970	5.99	+	2
NM_005641	cercaexons	terminal	4960	5155	7.86	+	2
NM_005641	cercaexons	internal	4960	5207	15.85	+	1
NM_005641	cercaexons	internal	4960	5210	15.24	+	1
NM_005641	cercaexons	internal	4960	5273	25.80	+	1
NM_005641	cercaexons	internal	4960	5303	28.63	+	1
NM_005641	cercaexons	terminal	4960	5378	25.36	+	1
NM_005641	cercaexons	first   	4977	5207	10.10	+	0
NM_005641	cercaexons	first   	4977	5210	9.49	+	0
NM_005641	cercaexons	first   	4977	5273	20.06	+	0
NM_005641	cercaexons	first   	4977	5303	22.89	+	0
NM_005641	cercaexons	single  	4977	5378	19.62	+	0
NM_005641	cercaexons	internal	4980	4994	6.14	-	2
NM_005641	cercaexons	internal	4980	4994	5.76	-	1
NM_005641	cercaexons	internal	4980	4994	5.01	-	0
NM_005641	cercaexons	internal	4980	5248	6.52	-	1
NM_005641	cercaexons	terminal	5089	5155	6.21	+	2
NM_005641	cercaexons	internal	5089	5207	13.07	+	1
NM_005641	cercaexons	internal	5089	5210	12.46	+	1
NM_005641	cercaexons	internal	5089	5273	23.03	+	1
NM_005641	cercaexons	internal	5089	5303	25.86	+	1
NM_005641	cercaexons	terminal	5089	5378	22.59	+	1
NM_005641	cercaexons	terminal	5126	5155	4.30	+	0
NM_005641	cercaexons	internal	5126	5207	13.60	+	2
NM_005641	cercaexons	internal	5126	5210	12.99	+	2
NM_005641	cercaexons	internal	5126	5273	23.56	+	2
NM_005641	cercaexons	internal	5126	5303	26.39	+	2
NM_005641	cercaexons	terminal	5126	5378	23.12	+	2
NM_005641	cercaexons	first   	5148	5207	8.40	+	0
NM_005641	cercaexons	first   	5148	5210	7.79	+	0
NM_005641	cercaexons	first   	5148	5273	18.36	+	0
NM_005641	cercaexons	first   	5148	5303	21.19	+	0
NM_005641	cercaexons	single  	5148	5378	17.92	+	0
NM_005641	cercaexons	first   	5202	5273	11.14	+	0
NM_005641	cercaexons	first   	5202	5303	13.98	+	0
NM_005641	cercaexons	single  	5202	5378	10.70	+	0
NM_005641	cercaexons	first   	5223	5273	7.90	+	0
NM_005641	cercaexons	first   	5223	5303	10.73	+	0
NM_005641	cercaexons	single  	5223	5378	7.46	+	0
NM_005641	cercaexons	internal	5238	5303	6.77	+	0
NM_005641	cercaexons	internal	5403	5489	11.26	+	0
NM_005641	cercaexons	internal	5403	5517	12.37	+	0
NM_005641	cercaexons	internal	5403	5639	13.26	+	0
NM_005641	cercaexons	internal	5403	5677	10.30	+	0
NM_005641	cercaexons	terminal	5403	5687	10.28	+	0
NM_005641	cercaexons	first   	5418	5489	7.68	+	0
NM_005641	cercaexons	first   	5418	5517	8.79	+	0
NM_005641	cercaexons	first   	5418	5639	9.68	+	0
NM_005641	cercaexons	first   	5418	5677	6.72	+	0
NM_005641	cercaexons	single  	5418	5687	6.70	+	0
NM_005641	cercaexons	first   	5424	5489	7.45	+	0
NM_005641	cercaexons	first   	5424	5517	8.56	+	0
NM_005641	cercaexons	first   	5424	5639	9.46	+	0
NM_005641	cercaexons	first   	5424	5677	6.49	+	0
NM_005641	cercaexons	single  	5424	5687	6.47	+	0
NM_005641	cercaexons	internal	5537	5639	6.84	+	0
NM_005641	cercaexons	internal	5537	5677	7.26	+	0
NM_005641	cercaexons	internal	5537	5716	10.90	+	0
NM_005641	cercaexons	internal	5537	5741	16.08	+	0
NM_005641	cercaexons	internal	5537	5762	15.40	+	0
NM_005641	cercaexons	internal	5537	5792	20.78	+	0
NM_005641	cercaexons	internal	5537	5796	19.65	+	0
NM_005641	cercaexons	terminal	5537	5800	18.25	+	0
NM_005641	cercaexons	internal	5541	5639	8.51	+	1
NM_005641	cercaexons	internal	5541	5677	8.94	+	1
NM_005641	cercaexons	internal	5541	5716	12.58	+	1
NM_005641	cercaexons	internal	5541	5741	17.76	+	1
NM_005641	cercaexons	internal	5541	5762	17.08	+	1
NM_005641	cercaexons	internal	5541	5792	22.46	+	1
NM_005641	cercaexons	internal	5541	5796	21.33	+	1
NM_005641	cercaexons	terminal	5541	5800	19.93	+	1
NM_005641	cercaexons	internal	5588	5639	10.53	+	0
NM_005641	cercaexons	internal	5588	5639	5.55	+	2
NM_005641	cercaexons	internal	5588	5677	10.95	+	0
NM_005641	cercaexons	internal	5588	5716	14.59	+	0
NM_005641	cercaexons	internal	5588	5741	19.77	+	0
NM_005641	cercaexons	internal	5588	5762	19.09	+	0
NM_005641	cercaexons	internal	5588	5792	24.47	+	0
NM_005641	cercaexons	internal	5588	5796	23.34	+	0
NM_005641	cercaexons	terminal	5588	5800	21.94	+	0
NM_005641	cercaexons	internal	5612	5639	5.80	+	0
NM_005641	cercaexons	internal	5612	5677	6.22	+	0
NM_005641	cercaexons	internal	5612	5716	9.86	+	0
NM_005641	cercaexons	internal	5612	5741	15.04	+	0
NM_005641	cercaexons	internal	5612	5762	14.36	+	0
NM_005641	cercaexons	internal	5612	5792	19.74	+	0
NM_005641	cercaexons	internal	5612	5796	18.61	+	0
NM_005641	cercaexons	terminal	5612	5800	17.21	+	0
NM_005641	cercaexons	internal	5742	5792	5.43	+	1
NM_005641	cercaexons	internal	5742	5796	4.31	+	1
NM_005641	cercaexons	internal	5742	5847	9.04	+	2
NM_005641	cercaexons	internal	5742	5847	8.58	+	0
NM_005641	cercaexons	terminal	5742	5933	6.09	+	0
NM_005641	cercaexons	first   	5791	5847	5.63	+	0
NM_005641	cercaexons	internal	5873	5931	6.54	-	0
NM_005641	cercaexons	first   	5891	6008	7.65	+	0
NM_005641	cercaexons	single  	5891	6076	5.34	+	0
NM_005641	cercaexons	internal	5920	5931	5.78	-	1
NM_005641	cercaexons	internal	5920	5931	4.01	-	0
NM_005641	cercaexons	internal	5952	6008	11.70	+	1
NM_005641	cercaexons	internal	5952	6008	8.56	+	2
NM_005641	cercaexons	terminal	5952	6076	9.38	+	1
NM_005641	cercaexons	terminal	6009	6200	5.56	+	0
NM_005641	cercaexons	single  	6018	6200	5.70	+	0
NM_005641	cercaexons	terminal	6078	6204	4.93	+	2
NM_005641	cercaexons	terminal	6082	6200	4.62	+	1
NM_005641	cercaexons	terminal	6082	6204	5.77	+	0
NM_005641	cercaexons	internal	6082	6246	4.20	+	2
NM_005641	cercaexons	internal	6339	6482	9.99	+	2
NM_005641	cercaexons	internal	6339	6529	18.74	+	2
NM_005641	cercaexons	internal	6339	6559	24.38	+	2
NM_005641	cercaexons	terminal	6339	6573	19.21	+	2
NM_005641	cercaexons	internal	6353	6482	7.38	+	1
NM_005641	cercaexons	internal	6353	6529	16.14	+	1
NM_005641	cercaexons	internal	6353	6559	21.78	+	1
NM_005641	cercaexons	terminal	6353	6573	16.61	+	1
NM_005641	cercaexons	first   	6355	6482	9.35	+	0
NM_005641	cercaexons	first   	6355	6529	18.10	+	0
NM_005641	cercaexons	first   	6355	6559	23.74	+	0
NM_005641	cercaexons	single  	6355	6573	18.58	+	0
NM_005641	cercaexons	first   	6400	6482	8.77	+	0
NM_005641	cercaexons	first   	6400	6529	17.53	+	0
NM_005641	cercaexons	first   	6400	6559	23.17	+	0
NM_005641	cercaexons	single  	6400	6573	18.00	+	0
NM_005641	cercaexons	terminal	6440	6481	5.94	+	0
NM_005641	cercaexons	internal	6440	6482	8.74	+	1
NM_005641	cercaexons	internal	6440	6529	17.50	+	1
NM_005641	cercaexons	internal	6440	6559	23.14	+	1
NM_005641	cercaexons	terminal	6440	6573	17.97	+	1
NM_005641	cercaexons	internal	6449	6529	11.68	+	1
NM_005641	cercaexons	internal	6449	6559	17.32	+	1
NM_005641	cercaexons	terminal	6449	6573	12.15	+	1
NM_005641	cercaexons	internal	6646	6683	5.11	+	2
NM_005641	cercaexons	internal	6650	6683	4.32	+	0
NM_005641	cercaexons	internal	6670	6683	4.97	+	2
NM_005641	cercaexons	internal	6719	6767	4.81	+	1
NM_005641	cercaexons	internal	6811	6904	4.51	+	2
NM_005641	cercaexons	internal	6814	6904	5.67	+	0
NM_005641	cercaexons	internal	6814	6904	5.21	+	2
NM_005641	cercaexons	internal	6840	6904	4.33	+	1
NM_005641	cercaexons	internal	6891	6955	5.81	+	0
NM_005641	cercaexons	internal	6891	7032	5.74	+	0
NM_005641	cercaexons	terminal	6891	7037	4.69	+	0
NM_005641	cercaexons	internal	6905	6955	4.53	+	2
NM_005641	cercaexons	internal	6905	7032	4.46	+	2
NM_005641	cercaexons	first   	6917	6955	5.67	+	0
NM_005641	cercaexons	first   	6917	7032	7.80	+	0
NM_005641	cercaexons	single  	6917	7054	6.87	+	0
NM_005641	cercaexons	first   	6930	6955	4.00	+	0
NM_005641	cercaexons	internal	6935	7080	4.45	-	2
NM_005641	cercaexons	internal	6950	7080	4.54	-	2
NM_005641	cercaexons	internal	6983	7080	5.70	-	0
NM_005641	cercaexons	internal	6983	7080	4.39	-	2
NM_005641	cercaexons	internal	7021	7249	6.30	+	0
NM_005641	cercaexons	terminal	7021	7281	4.26	+	0
NM_005641	cercaexons	internal	7029	7080	4.85	-	0
NM_005641	cercaexons	internal	7082	7222	11.57	+	0
NM_005641	cercaexons	internal	7082	7249	18.30	+	0
NM_005641	cercaexons	internal	7082	7249	4.95	+	1
NM_005641	cercaexons	terminal	7082	7336	18.47	+	0
NM_005641	cercaexons	internal	7104	7222	12.26	+	1
NM_005641	cercaexons	internal	7104	7249	18.99	+	1
NM_005641	cercaexons	internal	7104	7249	6.09	+	2
NM_005641	cercaexons	terminal	7104	7281	4.06	+	2
NM_005641	cercaexons	terminal	7104	7336	19.16	+	1
NM_005641	cercaexons	internal	7226	7334	4.73	-	1
NM_005641	cercaexons	internal	7358	7435	13.63	+	0
NM_005641	cercaexons	internal	7358	7435	8.32	+	1
NM_005641	cercaexons	internal	7358	7435	6.98	+	2
NM_005641	cercaexons	terminal	7358	7447	10.07	+	0
NM_005641	cercaexons	internal	7358	7482	8.26	+	1
NM_005641	cercaexons	internal	7358	7527	12.59	+	1
NM_005641	cercaexons	internal	7358	7539	10.18	+	1
NM_005641	cercaexons	internal	7358	7579	14.18	+	1
NM_005641	cercaexons	internal	7358	7584	15.19	+	1
NM_005641	cercaexons	internal	7358	7629	18.57	+	1
NM_005641	cercaexons	internal	7358	7690	20.68	+	1
NM_005641	cercaexons	internal	7358	7697	21.12	+	1
NM_005641	cercaexons	terminal	7358	7719	19.82	+	1
NM_005641	cercaexons	internal	7433	7527	4.96	+	1
NM_005641	cercaexons	internal	7433	7579	6.55	+	1
NM_005641	cercaexons	internal	7433	7584	7.56	+	1
NM_005641	cercaexons	internal	7433	7629	10.94	+	1
NM_005641	cercaexons	internal	7433	7690	13.05	+	1
NM_005641	cercaexons	internal	7433	7697	13.49	+	1
NM_005641	cercaexons	terminal	7433	7719	12.19	+	1
NM_005641	cercaexons	first   	7444	7527	5.41	+	0
NM_005641	cercaexons	first   	7444	7579	7.00	+	0
NM_005641	cercaexons	first   	7444	7584	8.01	+	0
NM_005641	cercaexons	first   	7444	7629	11.39	+	0
NM_005641	cercaexons	first   	7444	7690	13.50	+	0
NM_005641	cercaexons	first   	7444	7697	13.94	+	0
NM_005641	cercaexons	single  	7444	7719	12.64	+	0
NM_005641	cercaexons	internal	7463	7527	4.08	+	1
NM_005641	cercaexons	internal	7463	7579	5.67	+	1
NM_005641	cercaexons	internal	7463	7584	6.67	+	1
NM_005641	cercaexons	internal	7463	7629	10.05	+	1
NM_005641	cercaexons	internal	7463	7690	12.16	+	1
NM_005641	cercaexons	internal	7463	7697	12.61	+	1
NM_005641	cercaexons	terminal	7463	7719	11.30	+	1
NM_005641	cercaexons	internal	7474	7599	4.02	-	0
NM_005641	cercaexons	internal	7510	7579	5.57	+	0
NM_005641	cercaexons	internal	7510	7584	6.58	+	0
NM_005641	cercaexons	internal	7510	7629	9.96	+	0
NM_005641	cercaexons	internal	7510	7690	12.07	+	0
NM_005641	cercaexons	internal	7510	7697	12.51	+	0
NM_005641	cercaexons	terminal	7510	7719	11.21	+	0
NM_005641	cercaexons	internal	7528	7539	5.24	+	0
NM_005641	cercaexons	internal	7528	7539	4.79	+	1
NM_005641	cercaexons	internal	7528	7579	9.24	+	0
NM_005641	cercaexons	internal	7528	7584	10.25	+	0
NM_005641	cercaexons	internal	7528	7629	13.63	+	0
NM_005641	cercaexons	internal	7528	7629	5.03	+	1
NM_005641	cercaexons	internal	7528	7690	15.74	+	0
NM_005641	cercaexons	internal	7528	7697	16.18	+	0
NM_005641	cercaexons	terminal	7528	7719	14.88	+	0
NM_005641	cercaexons	first   	7576	7690	5.54	+	0
NM_005641	cercaexons	first   	7576	7697	5.99	+	0
NM_005641	cercaexons	single  	7576	7719	4.68	+	0
NM_005641	cercaexons	first   	7582	7629	4.21	+	0
NM_005641	cercaexons	first   	7582	7690	6.32	+	0
NM_005641	cercaexons	first   	7582	7697	6.76	+	0
NM_005641	cercaexons	single  	7582	7719	5.46	+	0
NM_005641	cercaexons	first   	7597	7690	4.85	+	0
NM_005641	cercaexons	first   	7597	7697	5.29	+	0
NM_005641	cercaexons	internal	7622	7690	4.20	+	1
NM_005641	cercaexons	internal	7622	7697	4.64	+	1
NM_005641	cercaexons	internal	7660	7690	7.83	+	0
NM_005641	cercaexons	internal	7660	7690	7.37	+	1
NM_005641	cercaexons	internal	7660	7697	8.51	+	1
NM_005641	cercaexons	internal	7660	7697	8.27	+	0
NM_005641	cercaexons	terminal	7660	7719	6.97	+	0
NM_005641	cercaexons	internal	7660	7745	11.33	+	1
NM_005641	cercaexons	terminal	7660	7787	10.63	+	1
NM_005641	cercaexons	internal	7667	7745	4.82	+	2
NM_005641	cercaexons	terminal	7667	7787	4.12	+	2
NM_005641	cercaexons	internal	7688	7745	5.60	+	2
NM_005641	cercaexons	terminal	7688	7787	4.91	+	2
NM_005641	cercaexons	internal	7874	7963	6.94	+	1
NM_005641	cercaexons	terminal	7874	8043	7.64	+	1
NM_005641	cercaexons	first   	7891	7963	4.79	+	0
NM_005641	cercaexons	single  	7891	8043	5.50	+	0
NM_005641	cercaexons	single  	7921	8043	4.35	+	0
NM_005641	cercaexons	terminal	8036	8048	4.80	+	2
NM_005641	cercaexons	internal	8036	8071	10.21	+	0
NM_005641	cercaexons	internal	8036	8143	11.20	+	0
NM_005641	cercaexons	internal	8036	8218	24.03	+	0
NM_005641	cercaexons	internal	8036	8268	22.20	+	0
NM_005641	cercaexons	internal	8036	8323	26.65	+	0
NM_005641	cercaexons	terminal	8036	8335	25.52	+	0
NM_005641	cercaexons	internal	8055	8218	15.09	+	1
NM_005641	cercaexons	internal	8055	8268	13.25	+	1
NM_005641	cercaexons	internal	8055	8323	17.71	+	1
NM_005641	cercaexons	terminal	8055	8335	16.58	+	1
NM_005641	cercaexons	first   	8060	8218	15.82	+	0
NM_005641	cercaexons	first   	8060	8268	13.99	+	0
NM_005641	cercaexons	first   	8060	8323	18.45	+	0
NM_005641	cercaexons	single  	8060	8335	17.32	+	0
NM_005641	cercaexons	internal	8100	8218	13.17	+	1
NM_005641	cercaexons	first   	8100	8218	4.22	+	0
NM_005641	cercaexons	internal	8100	8268	11.34	+	1
NM_005641	cercaexons	internal	8100	8323	15.79	+	1
NM_005641	cercaexons	terminal	8100	8335	14.67	+	1
NM_005641	cercaexons	internal	8153	8218	9.00	+	0
NM_005641	cercaexons	internal	8153	8268	7.17	+	0
NM_005641	cercaexons	internal	8153	8323	11.62	+	0
NM_005641	cercaexons	terminal	8153	8335	10.50	+	0
NM_005641	cercaexons	first   	8259	8356	4.95	-	0
NM_005641	cercaexons	first   	8273	8356	5.47	-	0
NM_005641	cercaexons	first   	8288	8356	4.08	-	0
NM_005641	cercaexons	terminal	8381	8432	4.97	+	2
NM_005641	cercaexons	terminal	8381	8484	4.40	+	1
NM_005641	cercaexons	terminal	8381	8524	6.14	+	0
NM_005641	cercaexons	internal	8412	8430	5.05	-	1
NM_005641	cercaexons	internal	8412	8460	4.11	-	1
NM_005641	cercaexons	internal	8574	8587	5.67	+	2
NM_005641	cercaexons	internal	8574	8587	4.70	+	0
NM_005641	cercaexons	internal	8574	8655	7.03	+	2
NM_005641	cercaexons	internal	8574	8655	7.01	+	1
NM_005641	cercaexons	terminal	8574	8659	6.79	+	1
NM_005641	cercaexons	terminal	8574	8709	4.80	+	2
NM_005641	cercaexons	internal	8764	8805	5.39	+	0
NM_005641	cercaexons	internal	8888	9045	5.25	-	1
NM_005641	cercaexons	internal	8888	9108	5.81	-	1
NM_005641	cercaexons	internal	8909	8950	7.06	+	0
NM_005641	cercaexons	internal	8909	8950	4.30	+	1
NM_005641	cercaexons	internal	8909	8952	4.12	+	0
NM_005641	cercaexons	internal	8992	9108	4.38	-	1
NM_005641	cercaexons	internal	9014	9045	4.15	-	1
NM_005641	cercaexons	internal	9014	9108	4.71	-	1
NM_005641	cercaexons	internal	9062	9204	4.43	+	0
NM_005641	cercaexons	terminal	9083	9144	5.91	+	1
NM_005641	cercaexons	internal	9083	9157	9.40	+	0
NM_005641	cercaexons	internal	9083	9157	4.61	+	2
NM_005641	cercaexons	terminal	9083	9203	7.80	+	2
NM_005641	cercaexons	internal	9083	9204	10.91	+	0
NM_005641	cercaexons	terminal	9083	9211	8.07	+	0
NM_005641	cercaexons	internal	9092	9108	8.58	-	1
NM_005641	cercaexons	internal	9092	9108	6.94	-	0
NM_005641	cercaexons	internal	9092	9108	5.25	-	2
NM_005641	cercaexons	internal	9092	9111	5.83	-	1
NM_005641	cercaexons	internal	9092	9111	5.08	-	0
NM_005641	cercaexons	internal	9092	9149	4.04	-	1
NM_005641	cercaexons	terminal	9135	9203	4.68	+	0
NM_005641	cercaexons	internal	9135	9204	4.24	+	1
NM_005641	cercaexons	internal	9183	9244	4.91	-	0
NM_005641	cercaexons	internal	9183	9244	4.42	-	1
NM_005641	cercaexons	internal	9183	9291	4.17	-	2
NM_005641	cercaexons	terminal	9284	9372	4.85	+	1
NM_005641	cercaexons	internal	9284	9409	22.43	+	0
NM_005641	cercaexons	terminal	9284	9445	21.91	+	0
NM_005641	cercaexons	internal	9317	9409	12.50	+	0
NM_005641	cercaexons	terminal	9317	9445	11.99	+	0
NM_005641	cercaexons	internal	9320	9409	11.21	+	0
NM_005641	cercaexons	terminal	9320	9445	10.69	+	0
NM_005641	cercaexons	internal	9550	9573	4.42	+	1
NM_005641	cercaexons	internal	10036	10073	6.74	+	0
NM_005641	cercaexons	internal	10036	10073	4.51	+	1
NM_005641	cercaexons	internal	10036	10142	8.74	+	0
NM_005641	cercaexons	first   	10036	10142	5.01	+	0
NM_005641	cercaexons	internal	10036	10142	4.13	+	1
NM_005641	cercaexons	terminal	10036	10146	6.82	+	0
NM_005641	cercaexons	internal	10060	10142	5.18	+	0
NM_005641	cercaexons	internal	10060	10142	4.38	+	2
NM_005641	cercaexons	internal	10111	10142	4.73	+	2
NM_005641	cercaexons	internal	10111	10142	4.58	+	0
NM_005641	cercaexons	internal	10199	10252	6.23	+	1
NM_005641	cercaexons	terminal	10199	10338	7.03	+	1
NM_005641	cercaexons	internal	10202	10252	5.81	+	1
NM_005641	cercaexons	terminal	10202	10338	6.62	+	1
NM_005641	cercaexons	terminal	10253	10300	5.18	+	0
NM_005641	cercaexons	terminal	10253	10338	4.88	+	1
NM_005641	cercaexons	internal	10398	10458	5.53	+	0
NM_005641	cercaexons	internal	10444	10458	4.33	+	2
NM_005641	cercaexons	internal	10447	10458	4.43	+	0
NM_005641	cercaexons	terminal	10527	10668	4.06	+	2
NM_005641	cercaexons	internal	10626	10918	6.47	+	1
NM_005641	cercaexons	internal	10626	10960	12.70	+	1
NM_005641	cercaexons	internal	10626	10990	15.60	+	1
NM_005641	cercaexons	internal	10626	11002	15.02	+	1
NM_005641	cercaexons	internal	10626	11041	12.99	+	1
NM_005641	cercaexons	internal	10626	11081	13.89	+	1
NM_005641	cercaexons	terminal	10626	11089	13.12	+	1
NM_005641	cercaexons	internal	10684	10918	11.46	+	2
NM_005641	cercaexons	internal	10684	10960	17.68	+	2
NM_005641	cercaexons	internal	10684	10990	20.59	+	2
NM_005641	cercaexons	internal	10684	11002	20.01	+	2
NM_005641	cercaexons	internal	10684	11041	17.97	+	2
NM_005641	cercaexons	internal	10684	11081	18.87	+	2
NM_005641	cercaexons	terminal	10684	11089	18.11	+	2
NM_005641	cercaexons	first   	10694	10918	8.45	+	0
NM_005641	cercaexons	first   	10694	10960	14.68	+	0
NM_005641	cercaexons	first   	10694	10990	17.58	+	0
NM_005641	cercaexons	first   	10694	11002	17.00	+	0
NM_005641	cercaexons	first   	10694	11041	14.97	+	0
NM_005641	cercaexons	first   	10694	11081	15.87	+	0
NM_005641	cercaexons	single  	10694	11089	15.10	+	0
NM_005641	cercaexons	first   	10704	10918	8.69	+	0
NM_005641	cercaexons	first   	10704	10960	8.95	+	0
NM_005641	cercaexons	single  	10704	10979	7.25	+	0
NM_005641	cercaexons	terminal	10730	10860	6.23	+	1
NM_005641	cercaexons	internal	10730	10918	10.62	+	2
NM_005641	cercaexons	internal	10730	10918	10.62	+	0
NM_005641	cercaexons	internal	10730	10960	16.84	+	0
NM_005641	cercaexons	internal	10730	10960	10.89	+	2
NM_005641	cercaexons	terminal	10730	10979	9.18	+	2
NM_005641	cercaexons	internal	10730	10990	19.75	+	0
NM_005641	cercaexons	internal	10730	11002	19.17	+	0
NM_005641	cercaexons	internal	10730	11041	17.13	+	0
NM_005641	cercaexons	internal	10730	11081	18.04	+	0
NM_005641	cercaexons	terminal	10730	11089	17.27	+	0
NM_005641	cercaexons	internal	10742	10918	7.71	+	2
NM_005641	cercaexons	internal	10742	10918	7.40	+	0
NM_005641	cercaexons	internal	10742	10960	13.63	+	0
NM_005641	cercaexons	internal	10742	10960	7.97	+	2
NM_005641	cercaexons	terminal	10742	10979	6.27	+	2
NM_005641	cercaexons	internal	10742	10990	16.53	+	0
NM_005641	cercaexons	internal	10742	11002	15.95	+	0
NM_005641	cercaexons	internal	10742	11041	13.92	+	0
NM_005641	cercaexons	internal	10742	11081	14.82	+	0
NM_005641	cercaexons	terminal	10742	11089	14.05	+	0
NM_005641	cercaexons	terminal	10782	10860	4.89	+	2
NM_005641	cercaexons	internal	10782	10918	11.13	+	1
NM_005641	cercaexons	internal	10782	10918	10.45	+	0
NM_005641	cercaexons	internal	10782	10960	17.35	+	1
NM_005641	cercaexons	internal	10782	10960	10.71	+	0
NM_005641	cercaexons	terminal	10782	10979	9.01	+	0
NM_005641	cercaexons	internal	10782	10990	20.26	+	1
NM_005641	cercaexons	internal	10782	11002	19.68	+	1
NM_005641	cercaexons	internal	10782	11041	17.64	+	1
NM_005641	cercaexons	internal	10782	11081	18.54	+	1
NM_005641	cercaexons	terminal	10782	11089	17.78	+	1
NM_005641	cercaexons	terminal	10817	10860	7.07	+	1
NM_005641	cercaexons	internal	10817	10918	12.58	+	0
NM_005641	cercaexons	internal	10817	10918	10.45	+	2
NM_005641	cercaexons	internal	10817	10960	18.81	+	0
NM_005641	cercaexons	internal	10817	10960	10.72	+	2
NM_005641	cercaexons	terminal	10817	10979	9.01	+	2
NM_005641	cercaexons	internal	10817	10990	21.71	+	0
NM_005641	cercaexons	internal	10817	11002	21.14	+	0
NM_005641	cercaexons	internal	10817	11041	19.10	+	0
NM_005641	cercaexons	internal	10817	11081	20.00	+	0
NM_005641	cercaexons	terminal	10817	11089	19.24	+	0
NM_005641	cercaexons	internal	10844	10960	9.13	+	0
NM_005641	cercaexons	internal	10844	10990	12.03	+	0
NM_005641	cercaexons	internal	10844	11002	11.45	+	0
NM_005641	cercaexons	internal	10844	11041	9.42	+	0
NM_005641	cercaexons	internal	10844	11081	10.32	+	0
NM_005641	cercaexons	terminal	10844	11089	9.55	+	0
NM_005641	cercaexons	internal	10868	10960	7.98	+	0
NM_005641	cercaexons	internal	10868	10990	10.88	+	0
NM_005641	cercaexons	internal	10868	11002	10.31	+	0
NM_005641	cercaexons	internal	10868	11041	8.27	+	0
NM_005641	cercaexons	internal	10868	11081	9.17	+	0
NM_005641	cercaexons	terminal	10868	11089	8.41	+	0
NM_005641	cercaexons	internal	10919	10960	12.67	+	0
NM_005641	cercaexons	internal	10919	10960	6.97	+	2
NM_005641	cercaexons	internal	10919	10960	5.84	+	1
NM_005641	cercaexons	terminal	10919	10979	5.26	+	2
NM_005641	cercaexons	internal	10919	10990	15.58	+	0
NM_005641	cercaexons	internal	10919	10990	7.62	+	1
NM_005641	cercaexons	internal	10919	11002	15.00	+	0
NM_005641	cercaexons	internal	10919	11002	6.33	+	1
NM_005641	cercaexons	terminal	10919	11019	4.12	+	1
NM_005641	cercaexons	internal	10919	11041	12.96	+	0
NM_005641	cercaexons	internal	10919	11081	13.86	+	0
NM_005641	cercaexons	terminal	10919	11089	13.10	+	0
NM_005641	cercaexons	internal	10952	10990	5.53	+	0
NM_005641	cercaexons	internal	10952	11002	4.95	+	0
NM_005641	cercaexons	internal	11006	11116	4.16	-	2
NM_005641	cercaexons	internal	11096	11131	4.66	+	0
NM_005641	cercaexons	terminal	11096	11194	4.34	+	0
NM_005641	cercaexons	internal	11115	11179	5.20	-	1
NM_005641	cercaexons	internal	11121	11179	6.63	-	0
NM_005641	cercaexons	internal	11121	11179	5.54	-	1
NM_005641	cercaexons	internal	11121	11196	5.36	-	1
NM_005641	cercaexons	internal	11121	11247	7.24	-	1
NM_005641	cercaexons	internal	11121	11328	6.60	-	1
NM_005641	cercaexons	first   	11121	11395	6.53	-	0
NM_005641	cercaexons	first   	11121	11434	6.14	-	0
NM_005641	cercaexons	internal	11181	11335	4.59	+	2
NM_005641	cercaexons	internal	11181	11392	4.69	+	2
NM_005641	cercaexons	internal	11181	11431	10.45	+	2
NM_005641	cercaexons	terminal	11181	11508	9.41	+	2
NM_005641	cercaexons	internal	11195	11431	9.27	+	1
NM_005641	cercaexons	terminal	11195	11508	8.23	+	1
NM_005641	cercaexons	internal	11198	11335	9.83	+	0
NM_005641	cercaexons	first   	11198	11335	7.57	+	0
NM_005641	cercaexons	internal	11198	11392	11.38	+	0
NM_005641	cercaexons	first   	11198	11392	9.13	+	0
NM_005641	cercaexons	internal	11198	11431	16.82	+	0
NM_005641	cercaexons	first   	11198	11431	14.56	+	0
NM_005641	cercaexons	internal	11198	11431	9.72	+	1
NM_005641	cercaexons	terminal	11198	11508	8.68	+	1
NM_005641	cercaexons	terminal	11198	11578	17.65	+	0
NM_005641	cercaexons	single  	11198	11578	15.40	+	0
NM_005641	cercaexons	terminal	11234	11249	5.86	+	2
NM_005641	cercaexons	internal	11234	11335	12.81	+	0
NM_005641	cercaexons	internal	11234	11335	7.25	+	1
NM_005641	cercaexons	internal	11234	11392	14.37	+	0
NM_005641	cercaexons	internal	11234	11392	7.34	+	1
NM_005641	cercaexons	internal	11234	11431	19.81	+	0
NM_005641	cercaexons	internal	11234	11431	13.10	+	1
NM_005641	cercaexons	terminal	11234	11508	12.06	+	1
NM_005641	cercaexons	terminal	11234	11578	20.64	+	0
NM_005641	cercaexons	terminal	11261	11297	4.23	+	2
NM_005641	cercaexons	internal	11261	11335	7.99	+	0
NM_005641	cercaexons	internal	11261	11392	9.55	+	0
NM_005641	cercaexons	internal	11261	11431	14.98	+	0
NM_005641	cercaexons	internal	11261	11431	7.22	+	1
NM_005641	cercaexons	terminal	11261	11508	6.18	+	1
NM_005641	cercaexons	terminal	11261	11578	15.81	+	0
NM_005641	cercaexons	internal	11270	11335	4.94	+	0
NM_005641	cercaexons	internal	11270	11392	6.50	+	0
NM_005641	cercaexons	internal	11270	11431	11.94	+	0
NM_005641	cercaexons	internal	11270	11431	4.77	+	1
NM_005641	cercaexons	terminal	11270	11578	12.77	+	0
NM_005641	cercaexons	internal	11300	11431	8.52	+	0
NM_005641	cercaexons	internal	11300	11431	4.67	+	1
NM_005641	cercaexons	terminal	11300	11578	9.35	+	0
NM_005641	cercaexons	internal	11330	11431	9.31	+	0
NM_005641	cercaexons	internal	11330	11431	7.10	+	1
NM_005641	cercaexons	terminal	11330	11508	6.06	+	1
NM_005641	cercaexons	terminal	11330	11578	10.14	+	0
NM_005641	cercaexons	internal	11366	11431	5.43	+	0
NM_005641	cercaexons	internal	11366	11431	4.20	+	1
NM_005641	cercaexons	terminal	11366	11578	6.26	+	0
NM_005641	cercaexons	internal	11387	11431	4.10	+	1
NM_005641	cercaexons	internal	11387	11431	4.00	+	0
NM_005641	cercaexons	terminal	11387	11578	4.83	+	0
NM_005641	cercaexons	first   	11396	11431	4.99	+	0
NM_005641	cercaexons	single  	11396	11578	5.82	+	0
NM_005641	cercaexons	internal	11413	11431	7.93	+	2
NM_005641	cercaexons	internal	11413	11431	5.63	+	1
NM_005641	cercaexons	internal	11413	11431	5.56	+	0
NM_005641	cercaexons	terminal	11413	11508	4.52	+	0
NM_005641	cercaexons	terminal	11413	11578	8.76	+	2
NM_005641	cercaexons	terminal	11413	11591	11.38	+	1
NM_005641	cercaexons	terminal	11429	11578	4.95	+	0
NM_005641	cercaexons	terminal	11429	11591	9.12	+	2
NM_005641	cercaexons	terminal	11432	11508	4.80	+	1
NM_005641	cercaexons	terminal	11432	11578	6.54	+	0
NM_005641	cercaexons	terminal	11432	11591	11.71	+	2
NM_005641	cercaexons	internal	11611	12153	7.21	+	1
NM_005641	cercaexons	internal	11611	12167	7.99	+	1
NM_005641	cercaexons	internal	11611	12272	5.88	+	1
NM_005641	cercaexons	internal	11621	12153	10.41	+	2
NM_005641	cercaexons	internal	11621	12167	11.19	+	2
NM_005641	cercaexons	internal	11621	12272	9.08	+	2
NM_005641	cercaexons	internal	11652	11825	6.01	+	2
NM_005641	cercaexons	internal	11652	11891	9.00	+	2
NM_005641	cercaexons	terminal	11652	12111	18.45	+	2
NM_005641	cercaexons	internal	11652	12153	5.70	+	0
NM_005641	cercaexons	internal	11652	12167	6.48	+	0
NM_005641	cercaexons	internal	11652	12272	4.36	+	0
NM_005641	cercaexons	internal	11677	11825	5.55	+	0
NM_005641	cercaexons	internal	11677	11891	8.54	+	0
NM_005641	cercaexons	terminal	11677	12111	18.00	+	0
NM_005641	cercaexons	internal	11677	12153	7.71	+	1
NM_005641	cercaexons	internal	11677	12167	8.48	+	1
NM_005641	cercaexons	internal	11677	12272	6.37	+	1
NM_005641	cercaexons	internal	11734	11825	9.79	+	0
NM_005641	cercaexons	internal	11734	11891	12.78	+	0
NM_005641	cercaexons	internal	11734	11891	4.67	+	1
NM_005641	cercaexons	terminal	11734	12111	22.23	+	0
NM_005641	cercaexons	internal	11734	12153	13.43	+	1
NM_005641	cercaexons	internal	11734	12153	9.70	+	2
NM_005641	cercaexons	internal	11734	12167	14.21	+	1
NM_005641	cercaexons	internal	11734	12167	9.82	+	2
NM_005641	cercaexons	terminal	11734	12190	6.56	+	2
NM_005641	cercaexons	internal	11734	12272	12.10	+	1
NM_005641	cercaexons	internal	11748	11825	4.06	+	2
NM_005641	cercaexons	internal	11748	11891	7.04	+	2
NM_005641	cercaexons	terminal	11748	12111	16.50	+	2
NM_005641	cercaexons	internal	11748	12153	8.08	+	0
NM_005641	cercaexons	internal	11748	12153	5.78	+	1
NM_005641	cercaexons	internal	11748	12167	8.86	+	0
NM_005641	cercaexons	internal	11748	12167	5.89	+	1
NM_005641	cercaexons	internal	11748	12272	6.75	+	0
NM_005641	cercaexons	internal	11768	11891	5.79	+	1
NM_005641	cercaexons	terminal	11768	12111	15.25	+	1
NM_005641	cercaexons	internal	11768	12153	6.36	+	2
NM_005641	cercaexons	internal	11768	12153	4.21	+	0
NM_005641	cercaexons	internal	11768	12167	7.13	+	2
NM_005641	cercaexons	internal	11768	12167	4.32	+	0
NM_005641	cercaexons	internal	11768	12272	5.02	+	2
NM_005641	cercaexons	internal	11808	11891	5.08	+	2
NM_005641	cercaexons	terminal	11808	12111	14.54	+	2
NM_005641	cercaexons	internal	11808	12153	7.59	+	1
NM_005641	cercaexons	internal	11808	12153	7.25	+	0
NM_005641	cercaexons	internal	11808	12167	8.02	+	0
NM_005641	cercaexons	internal	11808	12167	7.70	+	1
NM_005641	cercaexons	terminal	11808	12190	4.45	+	1
NM_005641	cercaexons	internal	11808	12272	5.91	+	0
NM_005641	cercaexons	terminal	11815	12111	11.60	+	0
NM_005641	cercaexons	internal	11815	12153	6.18	+	1
NM_005641	cercaexons	internal	11815	12153	5.95	+	2
NM_005641	cercaexons	internal	11815	12167	6.95	+	1
NM_005641	cercaexons	internal	11815	12167	6.07	+	2
NM_005641	cercaexons	internal	11815	12272	4.84	+	1
NM_005641	cercaexons	terminal	11859	12111	9.91	+	2
NM_005641	cercaexons	internal	11859	12153	10.89	+	1
NM_005641	cercaexons	internal	11859	12153	9.81	+	0
NM_005641	cercaexons	internal	11859	12167	11.01	+	1
NM_005641	cercaexons	internal	11859	12167	10.59	+	0
NM_005641	cercaexons	terminal	11859	12190	7.75	+	1
NM_005641	cercaexons	internal	11859	12272	8.47	+	0
NM_005641	cercaexons	terminal	11921	12111	11.22	+	1
NM_005641	cercaexons	internal	11921	12153	14.22	+	2
NM_005641	cercaexons	internal	11921	12153	11.55	+	0
NM_005641	cercaexons	internal	11921	12167	14.99	+	2
NM_005641	cercaexons	internal	11921	12167	11.66	+	0
NM_005641	cercaexons	terminal	11921	12190	8.41	+	0
NM_005641	cercaexons	internal	11921	12272	12.88	+	2
NM_005641	cercaexons	terminal	11960	12111	8.73	+	1
NM_005641	cercaexons	internal	11960	12153	13.98	+	2
NM_005641	cercaexons	internal	11960	12153	7.76	+	0
NM_005641	cercaexons	internal	11960	12167	14.76	+	2
NM_005641	cercaexons	internal	11960	12167	7.87	+	0
NM_005641	cercaexons	terminal	11960	12190	4.62	+	0
NM_005641	cercaexons	internal	11960	12272	12.64	+	2
NM_005641	cercaexons	terminal	11994	12111	8.57	+	2
NM_005641	cercaexons	internal	11994	12153	9.67	+	0
NM_005641	cercaexons	internal	11994	12153	8.85	+	1
NM_005641	cercaexons	internal	11994	12167	10.45	+	0
NM_005641	cercaexons	internal	11994	12167	8.96	+	1
NM_005641	cercaexons	terminal	11994	12190	5.71	+	1
NM_005641	cercaexons	internal	11994	12272	8.33	+	0
NM_005641	cercaexons	terminal	12000	12111	4.18	+	2
NM_005641	cercaexons	internal	12000	12153	4.99	+	0
NM_005641	cercaexons	internal	12000	12167	5.76	+	0
NM_005641	cercaexons	internal	12000	12167	4.02	+	1
NM_005641	cercaexons	internal	12047	12153	4.64	+	2
NM_005641	cercaexons	internal	12047	12167	5.42	+	2
NM_005641	cercaexons	terminal	12053	12111	5.51	+	1
NM_005641	cercaexons	internal	12053	12153	7.77	+	2
NM_005641	cercaexons	internal	12053	12153	5.14	+	0
NM_005641	cercaexons	internal	12053	12167	8.55	+	2
NM_005641	cercaexons	internal	12053	12167	5.25	+	0
NM_005641	cercaexons	internal	12053	12272	6.44	+	2
NM_005641	cercaexons	internal	12100	12153	4.58	+	1
NM_005641	cercaexons	internal	12100	12167	5.36	+	1

    Conclusions

    Hem pogut observar que el programa funciona correctament i que prediu tots els exons de les dues seqüències exemple. Un dels llocs de splicing de la seqüència NM_005641 tenia una puntuació molt dolenta i ens ha obligat a baixar el llindar fins a -2. Per trobar tots els exons d'un gen hem de jugar amb els diferents llindars fins a trobar-los tots, encara que és curiós veure com la màquinaria de splicing tria uns llocs de splicing en teoria pitjors tenint-ne d'altres millors, això ens indica que encara falta refinar molt els sistemes de predicció d'exons.

    Podríem pensar que les matrius de pesos utilitzades per a la realització del programa són prou correctes ja que han estat obtingudes a base de comparar molts llocs de splicing donadors i acceptors. No obstant, si enlloc d'utilitzar aquestes matrius utilitzéssim unes matrius de pesos condicionades, les prediccions serien més acurades. Aquest model s'anomena Model de Markov i les matrius utilitzades a l'hora de puntuar cada nucleòtid del possible lloc de splicing, tenen en compte les posicions anteriors. Hi ha diferents ordres; per exemple un Model de Markov d'ordre 2, a l'hora de puntuar una posició té en compte els nucleòtids de les dues posicions anteriors.

    El número d'exons predits sembla massa alt, però no ho és tant si pensem que estem predint els exons pels dos "strands" possibles, i que estem predint exons de tots els tipus (single, first, internal, terminal). A més a més si ensemblem aquests resultats per formar un gen, obtenim uns resultats prou satisfactoris. Per exemple podem utilitzar el servidor geneid del GRIB (Grup de Recerca en Informàtica Biomèdica de Barcelona).

    Per obtenir una representació gràfica dels resultats obtinguts podeu utilitzar el programa gff2ps

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