TBLASTN 2.7.1+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           10,816 sequences; 612,774,492 total letters



Query= SPP00000631_2.0 # Protein # Selenocysteine synthase (SecS) #
Zebrafish

Length=490
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

ML171014.1  Anarrhichthys ocellatus isolate YVR2014 unplaced geno...  165     2e-53


>ML171014.1 Anarrhichthys ocellatus isolate YVR2014 unplaced genomic scaffold 
scaffold11, whole genome shotgun sequence
Length=12673675

 Score = 165 bits (418),  Expect(2) = 2e-53, Method: Compositional matrix adjust.
 Identities = 87/130 (67%), Positives = 93/130 (72%), Gaps = 36/130 (28%)
 Frame = -2

Query  38       QGKCPEEGWSESTIELFLNELAVMDSNNFLGNCGVGEREGRVASSLVARRHY--------  89
                QGKCPE+GWSEST+ELFL+ELAVMDSNNFL NCGVGEREGRVASSLVARRHY        
Sbjct  7974150  QGKCPEDGWSESTVELFLSELAVMDSNNFLSNCGVGEREGRVASSLVARRHYRSKFDNH*  7973971

Query  90       ----------------------------RLIHGIGRSGDIAAVQPKAAGSSLLNKITNSV  121
                                            RLIHGIGRSGDIAAVQPKAAGSSLLNK+TNSV
Sbjct  7973970  LNDIPYLDASNLRRLVSRLLL*HPAVLCRLIHGIGRSGDIAAVQPKAAGSSLLNKLTNSV  7973791

Query  122      VLDVLKLTGV  131
                VLD+LKL+GV
Sbjct  7973790  VLDILKLSGV  7973761


 Score = 67.0 bits (162),  Expect(2) = 2e-53, Method: Compositional matrix adjust.
 Identities = 32/41 (78%), Positives = 34/41 (83%), Gaps = 0/41 (0%)
 Frame = -1

Query  1        MNSENFRLSEKLVSASYVRQGLDARRGHEQLIRRLLDQGKC  41
                MNSENF LSEK+VS SYVRQG  ARRGHEQLIR LL+Q K 
Sbjct  7974346  MNSENFSLSEKIVSTSYVRQGSQARRGHEQLIRHLLEQVKA  7974224


 Score = 149 bits (377),  Expect = 5e-37, Method: Compositional matrix adjust.
 Identities = 77/122 (63%), Positives = 82/122 (67%), Gaps = 35/122 (29%)
 Frame = -3

Query  182      AGFEPVVIENVLEGDELRTNLEEVERKIEEFGAENILCVHSTTSCFAPRVPD--------  233
                 GFEPVV+EN+LEGDELRT+LE VERKIEE GAENILCVHSTTSCFAPRVPD        
Sbjct  7972964  TGFEPVVVENILEGDELRTDLEAVERKIEELGAENILCVHSTTSCFAPRVPDR*HVFLIY  7972785

Query  234      ---------------------------RLEELSVLCAKHDIPHIVNNAYGVQSSKCMHLI  266
                                           RLEEL+  CAKH+IPHIVNNAYGVQSSKCMHLI
Sbjct  7972784  PF*KSSRCHSG*FINASEDKSIIILSYRLEELAAACAKHNIPHIVNNAYGVQSSKCMHLI  7972605

Query  267      QQ  268
                QQ
Sbjct  7972604  QQ  7972599


 Score = 117 bits (292),  Expect = 3e-26, Method: Compositional matrix adjust.
 Identities = 54/69 (78%), Positives = 60/69 (87%), Gaps = 0/69 (0%)
 Frame = -1

Query  122      VLDVLKLTGVRSVSSCFVVPMATGMSLTLCFLTLRHRRPKARYILWPRIDQKSCFKSMVT  181
                +L     TGVRSV+SCFVVPMATGMSLTLCFLTLRHRRPKARYI+WPRIDQKSCFK+M+T
Sbjct  7973335  MLRFFWTTGVRSVASCFVVPMATGMSLTLCFLTLRHRRPKARYIVWPRIDQKSCFKAMIT  7973156

Query  182      AGFEPVVIE  190
                AG + V I 
Sbjct  7973155  AGDDDVNIR  7973129


 Score = 91.7 bits (226),  Expect = 5e-18, Method: Compositional matrix adjust.
 Identities = 42/51 (82%), Positives = 47/51 (92%), Gaps = 0/51 (0%)
 Frame = -1

Query  263      MHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFDENFIQEISKIYPGR  313
                + +  QGARVGRIDAFVQSLDKNFMVPVGGAIIAGFDE+FIQEIS++YPG 
Sbjct  7972420  LAVFHQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFDESFIQEISQMYPGE  7972268


 Score = 88.2 bits (217),  Expect = 6e-17, Method: Compositional matrix adjust.
 Identities = 38/49 (78%), Positives = 45/49 (92%), Gaps = 0/49 (0%)
 Frame = -1

Query  403      ARVVPLGVQQTVSGHTFSGFMSHSEAYPCPYLNAASAIGITKGDVTVCM  451
                +RV+PLG +QTVSGH F GFMSHSEAYPCPYLNAASA+GIT+ DVT+C+
Sbjct  7968133  SRVIPLGKEQTVSGHRFLGFMSHSEAYPCPYLNAASAVGITREDVTLCI  7967987


 Score = 75.9 bits (185),  Expect = 6e-13, Method: Compositional matrix adjust.
 Identities = 48/104 (46%), Positives = 54/104 (52%), Gaps = 41/104 (39%)
 Frame = -1

Query  342      KELYGHLAQELSALAARHGERLLKTPHNPISL----------------------------  373
                +E+Y  LAQEL +LA+ HGERLL TPHNPISL                            
Sbjct  7969873  QEIYLFLAQELKSLASAHGERLLHTPHNPISLGKLNLLALDQQICTLYVLSMNATFPTLY  7969694

Query  374      -------------AMSLNHLEAHSSSAVTQLGSMLFTRQVSGAR  404
                             AMSL+ L+A S  AVTQLGSMLFTRQVSGAR
Sbjct  7969693  PYISHWVWLLSPPAMSLDGLQAESDKAVTQLGSMLFTRQVSGAR  7969562


 Score = 60.8 bits (146),  Expect = 3e-08, Method: Compositional matrix adjust.
 Identities = 29/32 (91%), Positives = 32/32 (100%), Gaps = 0/32 (0%)
 Frame = -1

Query  311      PGRASASPSLDVLITLLTLGANGYKKLLADRK  342
                PGRASASPSLDVLITLLTLGA+GYKKLL++RK
Sbjct  7971490  PGRASASPSLDVLITLLTLGASGYKKLLSERK  7971395



Lambda      K        H        a         alpha
   0.320    0.134    0.389    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 74060749860


  Database: genome.fa
    Posted date:  Oct 26, 2019  7:49 PM
  Number of letters in database: 612,774,492
  Number of sequences in database:  10,816



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40