TBLASTN 2.7.1+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           10,816 sequences; 612,774,492 total letters



Query= SPP00000644_2.0 # Protein # Methionine-R-sufoxide reductase 1b
(MSRB1b) # Zebrafish

Length=104
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

ML171007.1  Anarrhichthys ocellatus isolate YVR2014 unplaced geno...  93.2    4e-35
ML171065.1  Anarrhichthys ocellatus isolate YVR2014 unplaced geno...  79.0    3e-17


>ML171007.1 Anarrhichthys ocellatus isolate YVR2014 unplaced genomic scaffold 
scaffold4, whole genome shotgun sequence
Length=16548284

 Score = 93.2 bits (230),  Expect(2) = 4e-35, Method: Compositional matrix adjust.
 Identities = 40/49 (82%), Positives = 46/49 (94%), Gaps = 0/49 (0%)
 Frame = +2

Query  19        GIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMETLTAYKV  67
                 G+Y CSQC HPLFSSRSK+ HSSPWPAFT+T+REDSVTKMME+LTA+KV
Sbjct  12204053  GMYVCSQCNHPLFSSRSKFAHSSPWPAFTDTVREDSVTKMMESLTAFKV  12204199


 Score = 74.3 bits (181),  Expect(2) = 4e-35, Method: Compositional matrix adjust.
 Identities = 34/39 (87%), Positives = 37/39 (95%), Gaps = 0/39 (0%)
 Frame = +1

Query  66        KVLCGKCGNGLGHEFLNDGPEEGLSRFXIFSHSLKFVPK  104
                 +VLCGKCGNGLGHEF+NDGPEEG+SRF IFSHSLKFVP 
Sbjct  12204301  QVLCGKCGNGLGHEFVNDGPEEGVSRF*IFSHSLKFVPN  12204417


>ML171065.1 Anarrhichthys ocellatus isolate YVR2014 unplaced genomic scaffold 
scaffold62, whole genome shotgun sequence
Length=2282809

 Score = 79.0 bits (193),  Expect = 3e-17, Method: Compositional matrix adjust.
 Identities = 44/94 (47%), Positives = 52/94 (55%), Gaps = 25/94 (27%)
 Frame = -3

Query  1       MSFCSFFGKEFYKDHFKPG-------------------------IYACSQCGHPLFSSRS  35
               MS+CSF G E +K+HF+ G                         IY CS+CGH LFSS S
Sbjct  731078  MSYCSFSGGEEFKNHFQAGE*RSMKSHRSENESPFIGTFFFLSGIYVCSECGHELFSSMS  730899

Query  36      KYEHSSPWPAFTETIREDSVTKMMETLTAYKVLC  69
               K+EHSSPWPAF+ETI ED V+K  E     KV C
Sbjct  730898  KFEHSSPWPAFSETIHEDGVSKHPEAWGLLKVDC  730797


 Score = 77.0 bits (188),  Expect = 1e-16, Method: Compositional matrix adjust.
 Identities = 35/52 (67%), Positives = 41/52 (79%), Gaps = 0/52 (0%)
 Frame = -2

Query  16      FKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMETLTAYKV  67
               +  G+Y CS+C H LF+SRSK+EHSSPWPAFTETI EDSV+K  E   AYKV
Sbjct  732792  YSSGVYVCSKCDHQLFTSRSKFEHSSPWPAFTETIHEDSVSKHQERPGAYKV  732637


 Score = 62.8 bits (151),  Expect = 2e-11, Method: Compositional matrix adjust.
 Identities = 29/39 (74%), Positives = 32/39 (82%), Gaps = 0/39 (0%)
 Frame = -2

Query  66      KVLCGKCGNGLGHEFLNDGPEEGLSRFXIFSHSLKFVPK  104
               +V CGKCGNGLGHEFL DG  EGLSRF +FS SL F+PK
Sbjct  730665  QVCCGKCGNGLGHEFLYDGLTEGLSRF*LFSSSLTFIPK  730549


 Score = 54.7 bits (130),  Expect = 1e-08, Method: Composition-based stats.
 Identities = 31/40 (78%), Positives = 34/40 (85%), Gaps = 0/40 (0%)
 Frame = -2

Query  65      YKVLCGKCGNGLGHEFLNDGPEEGLSRFXIFSHSLKFVPK  104
               ++V CGKCGNGLGHEF+NDGP  G SRF IFS SLKFVPK
Sbjct  732447  FQVRCGKCGNGLGHEFVNDGPARGSSRF*IFSSSLKFVPK  732328


 Score = 38.9 bits (89),  Expect = 0.003, Method: Compositional matrix adjust.
 Identities = 17/21 (81%), Positives = 18/21 (86%), Gaps = 0/21 (0%)
 Frame = -1

Query  1       MSFCSFFGKEFYKDHFKPGIY  21
               MSFCSFFG E YKDHFKPG +
Sbjct  734644  MSFCSFFGGEVYKDHFKPGTF  734582



Lambda      K        H        a         alpha
   0.323    0.138    0.452    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 5085092500


  Database: genome.fa
    Posted date:  Oct 26, 2019  7:49 PM
  Number of letters in database: 612,774,492
  Number of sequences in database:  10,816



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40