_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 2e-17 Query name: gi|755469480|gb|AHB30480.2| thioredoxin reductase 3 [Oplegnathus fasciatus] Query range: 59-602 (total 602) Target: /data/www-rg/seblastian_results/97873130/tempfiles/temptarget.fasta Chromosome: ML683830.1:subseq_20410135,119414_ Strand: - Positions: -Exon 1 69259 69372 -Exon 2 68131 68247 -Exon 3 65917 65989 -Exon 4 63873 63992 -Exon 5 62678 62820 -Exon 6 60390 60505 -Exon 7 60042 60267 -Exon 8 59693 59785 -Exon 9 58995 59071 -Exon 10 55514 55670 -Exon 11 55181 55288 -Exon 12 51845 51940 -Exon 13 50003 50137 -Exon 14 49593 49658 Redox box: no Query IEDGTNYQEMLLEMTGQKTVPNVFINKTHIGGCDKTLQ <---Intron---> AHKDGSLQQLLSGE---NEAYDYDLIVIGGGSGGLACSK <---In //|| | | /| /|/ |/||||||/| ||/|| ||| | < 1011bp > |///||||//| | ||||||||||||||||||| < 21 Target LDDGPNIQRVLEDMSDQRTVPNVFVNGTHVGGYDKTFQ AYQNGSLQRVLEDNIDGAEVYDYDLIVIGGGSGGLACSK tgggcaaccgtggaagcaagcagtgagacgggtgaatc gtcagtccagcggaagggggtgtgcagagggtggcgtta taagcatagttaatgaagctcatttagcatggaaacta caaagctagttaaatagcataaaattttgggcggtcgca attccttgagaacgtcggtgctatgtaacgtatcattg tcgtcgtgggagtcattaatttctttttttcccatatcg Query tron---> EAALLGKKAMVLDYVVPTPKGTTWG <---Intron---> LGGTCVNVGCIPKKLMHQTALLGTAMQDARKFGWEFDETV <---Intro 41bp > ||| |||| |||||||||| ||/|| < 1924bp > |||||||||||||||||| |||| |/||/||||||/|| | < 1052b Target EAATLGKKVMVLDYVVPTPLGTSWG LGGTCVNVGCIPKKLMHQAALLGQALQDSRKFGWEYDEQV gggacgaagagcgtggcactgattg cggatgaggtacaacaccggccgcgccgtaatgtgtggcg accctgaattttaattccctgccg gtggcgtatggtcaattaaccttgactaacgatggaaaaa atttgaggtggatttaaatgttag attcttatatttggaggtatgtgagatgtagatcggttga Query n---> KHNWETMKMAVNNYIGSLNWGYRVALRDKNVNYVNAYAEFIEPHKIK <---Intron---> AINKRGKETFYTAAKFVLATGERPRYLGIPG p > ||||||| |/ ||||||||||||/||/| | |/||||||||||||| < 2172bp > | |//|/|||/|| |||||||||||||||| Target KHNWETMVEAIQNYIGSLNWGYRVSLREKTVTYLNAYAEFIEPHKIK ATNRKGQETFHTAQTFVLATGERPRYLGIPG acatgaagggacatagttatgtagttagaagatcagtggtagccaaa gaaaagcgatcagcatgcgaggaccttgacg taaagacttactaaatgctaggagtctgaactcatacacattacaata ccagagaactaccactttccgagcgatgtcg gaccggcgagctgccagagtgtcggggaagtggcctctaattgacaag accgaagactcaagatcaaaaagcatgtcta Query DKEYCITS <---Intron---> DDLFSLSYCPGKTLVIGASYVALECGGFLAGLGLDVTVMVRSILLRGFDQDMANRAGEHMEEHGVKFLRKYVPVK |||||||| < 122bp > |||||| |||||||//||||||||| |||||/||||||||||||||||||/|| ||| /|| |||||/|//||// Target DKEYCITS DDLFSLPYCPGKTLIVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQEMAERAGAYMETHGVKFIRRFVPIQ gagttaaa ggcttccttcgaacagggttggcgtggtcggagtggagagctacccgtgcgaggagggtagacggataaatgcac aaaagtcg aattctcagcgactttgccatctagcgttcgtgtatctttgctttggtaaatcagcgcatacagtattggttcta taacctt ctccccgtcctcatttgtcctgtggtaattttttgtcatggcccttactcaagggaatccgattttaccgatgttg Query <---Intron---> VEELEAGTPGRLKVTAKSTETDEIIEGEYNT <---Intron---> VLIAVGRDACTDKIGLDKTGVKVNPK <---Intron- < 256bp > /| || | |||||| |/||| ||/|||||| < 621bp > ||||/|||||| |||/| |||/| | < 3324bp Target IERLEEGMPGRLKVVAQSTEGPEIVEGEYNT VLIAIGRDACTKNIGLEKIGVKINEK agatgggacgacagggctaggcgaggggtaa gtagagcggtaaaagtgaaggaaaga tagtaagtcggtattcaccagcattagaaac tttctggacgcaatgtaatgtataaa tagggacgtaggggaagttaaaataaaattt tgatttttctcacttaagttcacta Query --> NGKIPVNDEEQTNVPHIYAIGDILEGKWELTPVAIQAGKLLARRLYGGSKLK <---Intron---> CDYVNVPTTVFTPLEYGACGLSEERATE > ||||||||||||||||/|||||||/|| |||||||||||||||||/|| /| < 225bp > ||||||||||||||||| ||| |||| / Target NGKIPVNDEEQTNVPHVYAIGDILDGKLELTPVAIQAGKLLARRLFGGGFIK CDYVNVPTTVFTPLEYGCCGLPEERAIK agaacgagggcaagccgtgaggatggacgcacggacggaccgcactgggtaa tgtgagcaagtactgtgttgtcggagaa agatctaaaaacatcatactgattagatatcctctacgattcggttgggtta gaatatcccttcctaaggggtcaagcta gtaattattgaactgttctctattgtgatatcatctgacgagtcgttttgtag tctctaatagtttgatcttaatggacca Query LYGKDNIE <---Intron---> VFHSLFWPLEFTVPNRDNNKCYGKIICNKLDS <---Intron---> DRVIGFHYLGPNAGEVTQGFGAAMKCGA |||/||| < 3240bp > |||/||||||/|| |||| || |||||| |/ < 1707bp > /|||||| |||||||/|||||||/||| Target EYGKENIE VFHTLFWPLEWTVAGRDNNTCYSKIICNKHDN NRVIGFHVLGPNAGEITQGFGAAIKCGL gtgagaag gtcatttccgtagggagaaatttaaataacga acgagtcgcgcagggaacgtgggaatgc aagaaata ttacttgctagctcggaaacgacattgaaaaa agttgtattgcacgatcagtgcctaggt atagacag gtctgtgttagaatcactttccagccctatcc ctaaattctacttgatcatttcaaatac Query TKEQLDSTIGIHPTCAE <---Intron---> IFTTLEVTKSSGGDITQAGCUG ||| |/ |||||||||| < 344bp > /|||||/||||| ||||||||| Target TKELLEETIGIHPTCAE VFTTLEITKSSGKDITQAGCUG aagtcggaagaccatgg gtaatgaaattgagaacggttg caattaactgtaccgca ttcctatcaccgaatcacgggg gaaataaaacacaatag cctagataattaatctgaccat * Sequence until next stop: TAA * Distance CDS-SECIS: 216 ; UGA-SECIS: 219 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = ML683830.1:subseq_20410135,119414_ Strand = - Positions = 49376 - 49281 Found by: Infernal (score=23.55) Covels (score=24.77) Stem2 length = 9 Apical loop length = 18 Insertion in stem2 = 0, 0 Free Energy = -11.56 ..(((((((.......................(((((((((((((((................)))))))))))))))..........))))))).. model uucggucaugcg----------------UuaAUGAcGgccuggcccuAAAcC-cuuuuuggggcgggccaggcCuGAUGuu-u-uuucaugaccggc .(({((({(((.....................{[[(((({((((((..................))))))}))))]]}......-.)))})))})). target UUGCUUGGAGGUAGCACAUAUAAGUGCUUUUUUGAAGUUUUGGCUCAGAACCCCAUUCUGGGGCGAGCUACGACUGAUGACUUA-CCUUGCAAACAU **** ^^ ****