_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:QUE_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 4e-39 Query name: gi|1002577066|ref|XP_015667711.1| PREDICTED: selenide, water dikinase 2 isoform X2 [Protobothrops mucrosquamatus] Query range: 25-366 (total 379) Target: /data/www-rg/seblastian_results/793197735/tempfiles/temptarget.fasta Chromosome: ML683863.1:subseq_1,63734_ Strand: + Positions: -Exon 1 6943 7162 -Exon 2 7642 7745 -Exon 3 8131 8238 -Exon 4 11249 11403 -Exon 5 11918 12008 -Exon 6 12257 12356 -Exon 7 12706 12953 Redox box: no Query ALPPGPPGYRPFEPEAVGLSPGWRLTSYGELRGUGCKVPQETLLRLLEGLGTSHAGLAGAGDTGAAGNPPPTLG <---Intron---> IGMDSCVIPL | ||||||||| |||/||/|||||| /||||||||||||||||||| ||| | /| || | / | < 479bp > |||||||||| Target AAAPGPPGYRPFAPEALGLAPGWRLTGFGELRGUGCKVPQETLLRLLAGLGGGGAAVAVAGGEPAGPSLGERGG IGMDSCVIPL gggcgccgtcctgcggcgcgcgtccagtggccgtgtagccgaccaccggcgggggggggggggcggctcggcgg agagttgacc ccccgccgagctccactgtccgggtcgtgatgggggatcaacttgttcgtggggcctctcggaccgcctgagg gtgtacgttct cgcccgcctgccgcgggcgctcgccgctgggccaccacgggggcgcggggcgcgctcgcggcgcggcggtggc ctggtcccttc * Query RHGGLSLVQTTDFFYPLVEDPYMM <---Intron---> GRIACANVLSDLYAMGITECDNMLMLLSISQKMTEE <---Intron---> EREKIMPL |||||||||||||||||||||||| < 385bp > ||||||||||||||||||||||||||||/|||||/| < 3010bp > |||||||| Target RHGGLSLVQTTDFFYPLVEDPYMM GRIACANVLSDLYAMGITECDNMLMLLSVSQKMTDE EREKIMPL ccggctcgcaagtttccgggctaa gcagtgagcagctgagaagtgaataccagacaaagg gcgaaact gaggtcttaccattacttaacatt ggtcgcattgatactgtcagaatttttgtgaatcaa agaattct gtgttctagtaccccagtgtccgg ctcctccgattcccggccgtctgggggtccgagttg gtagcgag Query IIKGFRDAAEDGGTSVTGGQTVLNPWIIVGGVATVVCQPNEFIM <---Intron---> PDSAVPGDVLVLTKPLGTQVAVNAHQWLDN <---Intro ||||||||||||||||||||||||||||/||||||||||||||| < 514bp > ||||||||||||||||||||||/|||||| < 248bp Target IIKGFRDAAEDGGTSVTGGQTVLNPWIIIGGVATVVCQPNEFIM PDSAVPGDVLVLTKPLGTQVAVSAHQWLDI aaagtcgggggggatgaggcagtactaaaggggaggtccagtaa cgaggcgggcgcaaccgacgggagcctcga ttagtgaccaaggcctcggacttacgtttggtccttgacaattt cagctcgattttcactgcatctgcaagtat acatcgttagttgaagacagtagtcgcctgctttggtgccacc gactgttatgggccggacaggtccccgggtc Query n---> PERWNKIKLVVSKEDVELAYQEAMFSMAMLNRTA <---Intron---> ASLMHTFNAHAATDITGFGILGHAQNLAKQQRSEVSFVIHNLPI > ||||||||||||//|||||||||||||||||||| < 349bp > |||||||||||||||||||||||||||||||||||||||||||/ Target PERWNKIKLVVSRDDVELAYQEAMFSMAMLNRTA ASLMHTFNAHAATDITGFGILGHAQNLAKQQRSEVSFVIHNLPV cgctaaaacggtaggggcgtcggataagacaaag gatacatagcggagaagtgacgcgcacgacccaggttgacaccg caggaatatttcgaatatcaaacttgtcttagc ccgttactacacccatcgtgttgacaatcaaaggatctttaatct tgggcgtggggcattgggccgacgctgcggcgt tccggctccttcccccactgcactcgcgcgggctacttcctcctc Query IAKMAAISKACGNRSRLLQGTSPETSGKLDKPGKEQAK |||||||||||||| |||||||||||/ / | / | Target IAKMAAISKACGNRFGLLQGTSPETSGRAESQGPKNCK agaaggaaagtgactgcccgatcgatgaggacgcaata tcatcctgacggagtgttagcccaccggcagagcaaga tcggtcccgctgcgtagggcacggaatgagcgtcgtca Sequence until next stop: TGGTTTTGTAATTCACCTCACCATGGCCAGTTGGAAACTGCTGTCTCTCATCATCAGGCCTGCACATCCCCCAGGGACCTTCGGGGAAGCTGCTGGGACAATTTTGTAGATCAGAAGCTTGGCATCTCTAGGGTGAAGCTGGGACAAGACAACATGCATTTGGTGCTAGAGCCTCATGTGGGACAGCCTGTGACCAGAATGGGAGGCCTAGGTGTTGAGGGAGAGATGCCTGTGACTAACCTGGCAGAGCAAAATCTAGTACTTTCTTTTTCCATTATTGACTGA W F C N S P H H G Q L E T A V S H H Q A C T S P R D L R G S C W D N F V D Q K L G I S R V K L G Q D N M H L V L E P H V G Q P V T R M G G L G V E G E M P V T N L A E Q N L V L S F S I I D * Distance CDS-SECIS: 1292 ; UGA-SECIS: 2216 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = ML683863.1:subseq_1,63734_ Strand = + Positions = 14246 - 14318 Found by: Infernal (score=29.83) Covels (score=18.32) Stem2 length = 8 Apical loop length = 20 Insertion in stem2 = 1, 0 Free Energy = -11.65 ..(((((((.......((((((((((.(((((...............)))))))))))))))........))))))).. model uucggucaugcgUuaAUGAcGgccug-gcccuAAAcCcuuuuuggggcgggccaggcCuGAUGuuuuuucaugaccggc (({(((({(---....([[{(((((((.(........-............))))))))}]])..--....)}))))})) target UUGCUCUCU---UCAAUGAAGCUCCUCCCCUUAAACC-CCAUGAGGGCUAGGAGGAGUCGAGGA--CCCCAUAGAGGGA **** ^^ ****