_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:QUE_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 2e-14 Query name: gi|1007726120|ref|XP_015804284.1| PREDICTED: migration and invasion enhancer 1 [Nothobranchius furzeri] Query range: 8-43 (total 95) Target: /data/www-rg/seblastian_results/458445382/tempfiles/temptarget.fasta Chromosome: SSNR01000030.1:subseq_3848081,105065_ Strand: + Positions: -Exon 1 55065 55172 Redox box: no Query EYCGGUGYGPRYQELAQVIKGEFPEVDVSGFVGRRG / ||||/ ||/|/| /|| /||| |||| ||||| Target SFSGGUGHFPRFQDLMTLIKAKFPETDVSGNVGRRG ttaggtgctcctcgcaacaagatcgaggtgaggaag ctgggggatcgtaattcttacatcacatcgatgggg ttttaaactcgtgtcggtcatgttattgtctgaaat * Sequence until next stop: AAATAA K * Distance CDS-SECIS: 467 ; UGA-SECIS: 557 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = SSNR01000030.1:subseq_3848081,105065_ Strand = + Positions = 55640 - 55715 Found by: Infernal (score=26.87) Covels (score=19.28) Stem2 length = 11 Apical loop length = 10 Insertion in stem2 = 0, 0 Free Energy = -24.79 ..(((((((........(((((((((((((((...............)))))))))))))))...........))))))).. model uucggucaugcg-UuaAUGAcGgccuggcccuAAAcCcuuuuuggggcgggccaggcCuGAUGuu--u-uuucaugaccggc ((((((((((.......([[{(((((((((((........-----..)))))))))))}]]).......-..)))))))))) target GAGCUUCAGGCUGUCAAUGAUUACCGUGGCUUCAAAUCUU-----AAAGGUUAUGGUAUGAAGAAUCUA-UUCCUGAAGUUC **** ^^^ **** _________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:2 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 4e-16 Query name: gi|835924519|ref|XP_004552472.2| PREDICTED: migration and invasion enhancer 1 [Maylandia zebra] Query range: 2-95 (total 95) Target: /data/www-rg/seblastian_results/458445382/tempfiles/temptarget.fasta Chromosome: SSNR01000030.1:subseq_3848081,105065_ Strand: + Positions: -Exon 1 50000 50123 -Exon 2 50275 50351 -Exon 3 50432 50512 Redox box: no Query GVKVRVEYCGGUGYEPRYRELARVVKGEFSDADVTGFVGRTG <---Intron---> SFEIEINGQLVFSKVETGGFPYEDD <---Intron---> V /|| ||||||||||||||||/ |||||||/||||| < 151bp > |||/ |||||||||/|| ||||||| < 80bp > / Target ALKVFSRCSGGUGYEPRYRELARVVQDEFSDADVSGFVGRLA SFEVTINGQLVFSKLETKGFPYEDD I gtagttctaggtgtgcctcgcgcggcggttggggtgtggacg atggaaagccgttacgaagtctggg a ctattcgggggggaacgagatcgttaaatcacatcgttggt cgtatctagatttcatacagtcaaaa t tagatttcttaaacacctggccgtcgtgtctgtgactgaaa cctgtgccggttccgtgagccgtgtc c * Query MNAIQNAYDGKPLQKITKSRAPCVIM ||||||||||||/||||||||||||| Target MNAIQNAYDGKPVQKITKSRAPCVIM aagacagtggacgcaaaaacgctgaa tactaacaagactaatcaggccgttt gtacgttttgatgggccaccagtccg Sequence until next stop: TAA * Distance CDS-SECIS: 197 ; UGA-SECIS: 443 ------- SECIS element -- id:2 -- grade:A ---- Chromosome = SSNR01000030.1:subseq_3848081,105065_ Strand = + Positions = 50710 - 50785 Found by: Infernal (score=26.41) Covels (score=22.53) Stem2 length = 10 Apical loop length = 12 Insertion in stem2 = 0, 0 Free Energy = -15.69 ..(((((((........(((((((((((((((...............)))))))))))))))...........))))))).. model uucggucaugcg-UuaAUGAcGgccuggcccuAAAcCcuuuuuggggcgggccaggcCuGAUGuu--u-uuucaugaccggc ..({((((((.......([[{((((((((((.........-----...))))))))))}]]).......-..))))))}).. target GUGCUUCAGGCUGUCAAUGAUUACUGUAGCUGAAAAUCUU-----AAAGGUUAUGGUAUGAAGAAUCCA-AUCCUGAAACUC **** ^^^ ****