TBLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: genome.fa 3,184 sequences; 1,209,001,349 total letters Query= SPP00001058_2.0 # Protein # Selenoprotein S (SELENOS) # Chicken Length=194 Score E Sequences producing significant alignments: (Bits) Value PTFA01000083.1 Casuarius casuarius isolate B32419 scaffold_82, w... 45.1 2e-10 >PTFA01000083.1 Casuarius casuarius isolate B32419 scaffold_82, whole genome shotgun sequence Length=3852796 Score = 45.1 bits (105), Expect(2) = 2e-10, Method: Compositional matrix adjust. Identities = 23/28 (82%), Positives = 23/28 (82%), Gaps = 0/28 (0%) Frame = -2 Query 140 QQEAESGASTSSAVPKSKPNKKPLRGGG 167 QQE ESGASTSS VPK K NKKPLR GG Sbjct 2012664 QQELESGASTSSTVPKPKSNKKPLRSGG 2012581 Score = 42.0 bits (97), Expect(2) = 2e-10, Method: Compositional matrix adjust. Identities = 19/21 (90%), Positives = 20/21 (95%), Gaps = 0/21 (0%) Frame = -3 Query 120 IEMWESMQEGKSYKGNLKLSQ 140 I MWESMQEGKSYKGNLKL+Q Sbjct 2012828 IAMWESMQEGKSYKGNLKLNQ 2012766 Score = 54.7 bits (130), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 24/27 (89%), Positives = 27/27 (100%), Gaps = 0/27 (0%) Frame = -3 Query 30 VGALLSSYGWYILLACVAIYLIVQKIS 56 VG+LLSSYGWYILLACVAIYL++QKIS Sbjct 2016197 VGSLLSSYGWYILLACVAIYLLIQKIS 2016117 Score = 50.1 bits (118), Expect = 1e-05, Method: Compositional matrix adjust. Identities = 23/28 (82%), Positives = 27/28 (96%), Gaps = 0/28 (0%) Frame = -2 Query 74 VEPDMVVRRQEALLASRLRMQEELNAQA 101 +EPD+VV+RQEAL A+RLRMQEELNAQA Sbjct 2014413 LEPDLVVKRQEALAAARLRMQEELNAQA 2014330 Score = 37.0 bits (84), Expect = 0.23, Method: Compositional matrix adjust. Identities = 15/15 (100%), Positives = 15/15 (100%), Gaps = 0/15 (0%) Frame = -1 Query 167 GYNPLSGEGGGTCSW 181 GYNPLSGEGGGTCSW Sbjct 2010565 GYNPLSGEGGGTCSW 2010521 Lambda K H a alpha 0.312 0.129 0.372 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 30599480012 Database: genome.fa Posted date: Oct 19, 2018 9:06 AM Number of letters in database: 1,209,001,349 Number of sequences in database: 3,184 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40