Command line: [exonerate --exhaustive yes -m p2g --showtargetgff -q ../GPx4-human.fa -t GPx4-NIVO01030317.1.subseq] Hostname: [sitdoc] C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 956 Query range: 0 -> 196 Target range: 48945 -> 51041 1 : MetSerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuAla : 20 ||||||!!!.!!||||||! !||||||||||||||||||||||||||||||||||||||| MetSerPheSerArgLeuTyrArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuAla 48946 : ATGAGCTTTAGCCGTCTGTACCGCCTGCTCAAGCCAGCGCTACTCTGCGGGGCTCTGGCT : 49003 21 : AlaProGlyLeuAlaGlyThrMet >>>> Target Intron 1 >>>> CysAlaS : 31 |||||||||||||||.!!|||||| 970 bp ||||||| AlaProGlyLeuAlaSerThrMet++ ++CysAlaS 49004 : GCGCCCGGCCTGGCCAGCACCATGgt.........................agTGCGCGT : 50006 32 : erArgAspAspTrpArgCysAlaArgSerMetHisGluPheSerAlaLysAspIleAspG : 51 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| erArgAspAspTrpArgCysAlaArgSerMetHisGluPheSerAlaLysAspIleAspG 50007 : CCCGCGACGACTGGCGCTGTGCTCGCTCCATGCACGAATTCTCAGCCAAGGACATCGACG : 50066 52 : lyHisMetValAsnLeuAspLysTyr{Ar} >>>> Target Intron 2 >>>> { : 60 ||!.!|||||||||||||||||||||{||} 77 bp { lyArgMetValAsnLeuAspLysTyr{Ar}++ ++{ 50067 : GGCGCATGGTTAACCTGGACAAGTAC{CG}gt.........................ag{ : 50172 61 : g}GlyPheValCysIleValThrAsnValAlaSerGlnUnkGlyLysThrGluValAsnT : 80 |}||| !|||||||||||||||||||||||||||||| |||||||||!!:||||||| g}GlyHisValCysIleValThrAsnValAlaSerGln***GlyLysThrAspValAsnT 50173 : G}GGCCACGTGTGCATCGTCACCAATGTGGCCTCGCAATGAGGCAAGACTGACGTAAACT : 50230 81 : yrThrGlnLeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArgIleLeuAlaP : 100 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| yrThrGlnLeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArgIleLeuAlaP 50231 : ACACTCAGCTGGTCGACCTGCACGCCCGATACGCCGAGTGTGGTTTACGGATCCTGGCCT : 50290 101 : heProCysAsnGlnPheGlyLysGln >>>> Target Intron 3 >>>> GluPr : 110 ||||||||||||||||||||!:!||| 138 bp ||||| heProCysAsnGlnPheGlyArgGln++ ++GluPr 50291 : TCCCTTGCAACCAGTTTGGGAGGCAGgt.........................agGAGCC : 50458 111 : oGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyrAsnValLysPheAspMetPh : 130 ||||||||||! !||||||||||||||||||||||||||||||||||||||||||:!!|| oGlySerAsnAlaGluIleLysGluPheAlaAlaGlyTyrAsnValLysPheAspLeuPh 50459 : AGGGAGTAATGCGGAGATCAAAGAGTTCGCTGCTGGCTACAACGTCAAATTCGATTTGTT : 50518 131 : eSerLysIleCysValAsnGlyAspAspAlaHisProLeuTrpLysTrpMetLysIleGl : 150 |||||||||||||||||||||||||||||||||||||||||||||||||||||||:!!|| eSerLysIleCysValAsnGlyAspAspAlaHisProLeuTrpLysTrpMetLysValGl 50519 : CAGCAAGATCTGTGTAAATGGGGACGACGCCCACCCTCTGTGGAAATGGATGAAAGTCCA : 50578 151 : nProLysGlyLysGlyIleLeuGly{As} >>>> Target Intron 4 >>>> {n : 159 ||||||||||!:!|||!!:||||||{||} 111 bp {| nProLysGlyArgGlyMetLeuGly{As}++ ++{n 50579 : GCCCAAGGGGAGGGGCATGCTGGGA{AA}gt.........................ag{C : 50718 160 : }AlaIleLysTrpAsnPheThrLys >>>> Target Intron 5 >>>> PheLeu : 169 }|||||||||||||||||||||||| 140 bp |||||| }AlaIleLysTrpAsnPheThrLys++ ++PheLeu 50719 : }GCCATCAAATGGAACTTCACCAAGgt.........................agTTCCTC : 50886 170 : IleAspLysAsnGlyCysValValLysArgTyrGlyProMetGluGluProLeu >>>> : 188 |||||||||||||||||||||||||||||||||||||||||||||||||||||| IleAspLysAsnGlyCysValValLysArgTyrGlyProMetGluGluProLeu++ 50887 : ATTGATAAGAACGGCTGTGTGGTGAAGCGGTATGGTCCCATGGAAGAGCCCCTGgt.... : 50945 189 : Target Intron 6 >>>> ValIleGluLysAspLeuProHisTyr : 196 72 bp ||||||||||||||||||||| !||| ++ValIleGluLysAspLeuProCysTyr 50946 : .....................agGTGATAGAGAAGGATCTGCCATGCTAC : 51041 vulgar: SPP00000007_1.0 0 196 . NIVO01030317.1:subseq(555646,1182752) 48945 51041 + 956 M 28 84 5 0 2 I 0 966 3 0 2 M 31 93 S 0 2 5 0 2 I 0 73 3 0 2 S 1 1 M 48 144 5 0 2 I 0 134 3 0 2 M 50 150 S 0 2 5 0 2 I 0 107 3 0 2 S 1 1 M 8 24 5 0 2 I 0 136 3 0 2 M 20 60 5 0 2 I 0 68 3 0 2 M 9 27 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 48946 51041 956 + . gene_id 1 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 48946 49029 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 48946 49029 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 49030 49031 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 49030 49999 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 49998 49999 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 50000 50094 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 50000 50094 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 50095 50096 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 50095 50171 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 50170 50171 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 50172 50316 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 50172 50316 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 50317 50318 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 50317 50454 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 50453 50454 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 50455 50606 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 50455 50606 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 50607 50608 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 50607 50717 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 50716 50717 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 50718 50742 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 50718 50742 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 50743 50744 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 50743 50882 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 50881 50882 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 50883 50942 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 50883 50942 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 50943 50944 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 50943 51014 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 51013 51014 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 51015 51041 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 51015 51041 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 48946 51041 956 + . alignment_id 1 ; Query SPP00000007_1.0 ; Align 48946 1 84 ; Align 50000 29 93 ; Align 50173 61 144 ; Align 50455 109 150 ; Align 50719 160 24 ; Align 50883 168 60 ; Align 51015 188 27 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 164 Query range: 3 -> 187 Target range: 12272 -> 1125868 4 : GlyArgLeuCys{Ar} >>>> Target Intron 1 >>>> {g}LeuLeuLy : 11 |||!:!||||||{||} 67457 bp {|}:!!||||| GlyGlnLeuCys{Ar}++ ++{g}IleLeuLy 12273 : GGCCAGCTCTGC{CG}gt.........................ag{G}ATTCTGAA : 79751 12 : sPro >>>> Target Intron 2 >>>> AlaLeuLeuCysGlyAlaLeu{A : 20 | ! 74790 bp |||! !|||||| ! !!! !{| sLys++ ++AlaArgLeuCysLysProPro{A 79752 : AAAGgt.........................agGCCCGCTTGTGCAAGCCCCCG{G : 154568 21 : } >>>> Target Intron 3 >>>> {la}AlaProGlyLeuAlaGlyThrM : 28 } 133100 bp {||}::!|||.!!:!!||||||||| }++ ++{la}SerProSerValAlaGlyThrA 154569 : }gt.........................ag{CC}AGCCCAAGTGTGGCAGGCACTG : 287692 29 : et{Cy} >>>> Target Intron 4 >>>> {s}AlaSerArgAspAspTrp : 35 {||} 36905 bp {|}|||!!!|||!!:|||!:: sp{Cy}++ +-{s}AlaThrArgGluAspTyr 287693 : AC{TG}gt.........................at{C}GCCACCAGAGAGGACTAC : 324618 36 : {Ar} >>>> Target Intron 5 >>>> {g}CysAlaArgSerMet{Hi} : 42 {||} 18266 bp {|}! !.!!|||:!! { } {Ar}++ ++{g}TyrThrArgAlaTyr{Va}+ 324619 : {AG}gt.........................ag{A}TACACAAGAGCATAC{GT}g : 342907 43 : >>>> Target Intron 6 >>>> {s}GluPheSer{Al} >>>> Targe : 46 158758 bp { }|||||||||{ } 2 + ++{l}GluPheSer{Ar}++ 342908 : t.........................ag{g}gaattttcc{ag}gt.......... : 501679 47 : t Intron 7 >>>> {a}LysAspIleAspGlyHisMetValAsnLeuAspLys : 58 5521 bp {!}|||! :!!! !|||..!:!! |||...::: -+{g}LysGlyValGlyGlnHisGlnLeuCysLeuGlnGlu 501680 : ...............gg{G}AAGGGAGTCGGGCAGCATCAGCTGTGcctgcaggag : 527232 59 : TyrArgGlyPheValCysIleValThrAsnValAlaSerGln >>>> Target : 73 |||||| ..! !|||..!.!!...|||::!!!!||| 717 GlyArgGlyThrThrHisIleThrAlaGlyValSerThrGln+- 527233 : gggagggggaccaCTCACATCACCGCAGGGGTGAGCACACAGga............ : 527279 74 : Intron 8 >>>> UnkGlyLysThrGluValAsnTyrThrGlnLeuValAspLe : 86 9 bp |||::: ! !|||!::|||::! !:!! ! ++GluGlyArgLeuProArgAsnTrpThrArgArgLeuProLy 527280 : .............agGAGGGTCGTCTTCCCAGGAACTGGACCAGAAGGCTCCCCAA : 534495 87 : uHisAlaArgTyrAla >>>> Target Intron 9 >>>> GluCysGlyLe : 95 !:!! !|||||| ! 26882 bp ! !..!!.! sTyrLeuArgTyrGln++ -+AlaAlaAlaAl 534496 : GTACCTCAGGTACCAGgt.........................ggGCGGCCGCCGC : 561404 96 : uArgIleLeuAlaPheProCysAsnGlnPheGlyLysGlnGluPro{G} >>>> : 111 ! !.!!||| ! ! !...||| ! |||||| :!!|||{|} aValPheLeuLeuGlyHisAlaAsnAlaSerGlyLysThrLysPro{G}++ 561405 : TGTCTTCTTGCTGGGACATGCGAACGCTTCAGGGAAAACCAAACCG{G}gt..... : 561455 112 : Target Intron 10 >>>> {ly}SerAsnGluGluIleLys >>>> Targ : 118 5253 bp {||}||| !:!!:!!:!! ++{ly}SerAlaLeuGlnLeuGln++ 561456 : .....................ag{gA}AGCGCATTGCAGCTTCAGgt......... : 566728 119 : et Intron 11 >>>> GluPheAla{Al} >>>> Target Intron 12 : 121 17699 bp |||||||||{!.} 18045 bp ++GluPheAla{Gl}++ 566729 : .................agGAGtttgca{gg}gt...................... : 584438 122 : >>>> {a}{G} >>>> Target Intron 13 >>>> {ly}TyrAsn{Va} : 125 {!}{|} 26284 bp {||}|||:::{ } ++{y}{G}++ ++{ly}TyrSer{Ar} 584439 : ....ag{g}{g}gt..........................ag{ga}tactcc{ag} : 628775 126 : >>>> Target Intron 14 >>>> {l}LysPheAspMetPheSerLys : 133 498 bp {!}! |||.!. ||||||||| +- ++{g}ThrPheGlnCysPheSerLys++ 628776 : gc..........................ag{G}ACCTTCCAGTGTTTTtcaaaagt : 629297 134 : >>>> Target Intron 15 >>>> IleCysValAsn >>>> Target In : 137 28275 bp |||:::||| 9074 ++SerCysMetAsn++ 629298 : ..........................agtcatgtatgaatgt.............. : 657586 138 : tron 16 >>>> >>>> Target Intron 17 >>>> GlyAspAspAla : 140 bp 44579 bp !|||||| ! ++++ ++GlnAspAspArg 657587 : ............aggt..........................agCAGGATGACAGG : 711246 141 : HisPro{Le} >>>> Target Intron 18 >>>> {u}TrpLys{Tr} > : 146 .!. !{ } 45618 bp {!}||| !{||} GluGly{Ar}++ ++{g}TrpLeu{Tr}++ 711247 : GAGGGA{AG}gt..........................ag{G}TGGCTA{TG}gt. : 756886 147 : >>> Target Intron 19 >>>> {p}MetLysIleGlnProLysGlyLysGl : 155 111802 bp {|} ... :::|||:::||| !.. ++{p}ArgSerSerArgProArgGlyGlyAs 756887 : .........................ag{g}agatcttcccgaccgaggggtGGAAa : 868711 156 : yIleLeuGlyAsnAlaIleLysTrpAsn >>>> Target Intron 20 >>>> : 165 . ||| ... ::: |||... 93029 bp nCysLeuLeuArgLeuLeuHisTrpGln++ 868712 : ctgtctcttacgtctcctgcactggcaggt.......................... : 868743 166 : PheThrLysPheLeu{Il} >>>> Target Intron 21 >>>> {e} : 171 |||!..!:::!!{!!} 20520 bp {:} ++AlaThrSerTrpVal{Me}++ ++{t}++ 868744 : agGCAACAAGCTGGGTG{AT}gt..........................ag{g}gt : 982308 172 : >>>> Target Intron 22 >>>> >>>> Target Intron 23 >>>> : 171 29069 bp 52744 bp ++++ 982309 : ..........................aggt.......................... : 1011379 172 : AspLysAsn >>>> Target Intron 24 >>>> GlyCysVal >>>> : 177 ...||| 30609 bp .!!|||..! ++GlySerAsn++ ++SerCysThr++ 1011380 : agggatcaaacgt..........................agAGCTGCACTgt.... : 1094750 178 : Target Intron 25 >>>> >>>> Target Intron 26 >>>> Va : 177 17400 bp 13688 bp || ++++ ++Va 1094751 : ......................aggt..........................agGT : 1125836 178 : lLysArgTyrGlyProMetGluGluProLeu : 187 |::: ! !|||||| ...! |||||| lArgAlaArgGlyProProArgAlaProLeu 1125837 : GCGTGCCCGTGGCCCGCCCCGTGCCCCTCTC : 1125868 vulgar: SPP00000007_1.0 3 187 . NIVO01030317.1:subseq(555646,1182752) 12272 1125868 + 164 M 4 12 S 0 2 5 0 2 I 0 67453 3 0 2 S 1 1 M 4 12 5 0 2 I 0 74786 3 0 2 M 7 21 S 0 1 5 0 2 I 0 133096 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 36901 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 18262 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 158754 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 25517 3 0 2 S 1 1 M 26 78 5 0 2 I 0 7175 3 0 2 M 19 57 5 0 2 I 0 26878 3 0 2 M 19 57 S 0 1 5 0 2 I 0 5249 3 0 2 S 1 2 M 6 18 5 0 2 I 0 17695 3 0 2 M 3 9 S 0 2 5 0 2 I 0 18041 3 0 2 S 1 2 5 0 2 I 0 26280 3 0 2 S 1 2 M 2 6 S 0 2 5 0 2 I 0 494 3 0 2 S 1 1 M 7 21 5 0 2 I 0 28271 3 0 2 M 4 12 5 0 2 I 0 9070 3 0 2 5 0 2 I 0 44575 3 0 2 M 6 18 S 0 2 5 0 2 I 0 45614 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 111798 3 0 2 S 1 1 M 18 54 5 0 2 I 0 93025 3 0 2 M 5 15 S 0 2 5 0 2 I 0 20516 3 0 2 S 1 1 5 0 2 I 0 29065 3 0 2 5 0 2 I 0 52740 3 0 2 M 3 9 5 0 2 I 0 30605 3 0 2 M 3 9 5 0 2 I 0 17396 3 0 2 5 0 2 I 0 13684 3 0 2 M 11 33 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 12273 1125868 164 + . gene_id 2 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 12273 12286 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 12273 12286 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 12287 12288 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 12287 79743 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 79742 79743 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 79744 79756 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 79744 79756 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 79757 79758 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 79757 154546 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 154545 154546 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 154547 154568 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 154547 154568 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 154569 154570 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 154569 287668 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 287667 287668 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 287669 287696 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 287669 287696 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 287697 287698 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 287697 324601 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 324600 324601 . + . intron_id 3 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 324602 324622 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 324602 324622 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 324623 324624 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 324623 342888 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 342887 342888 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 342889 342906 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 342889 342906 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 342907 342908 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 342907 501664 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 501663 501664 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 501665 501676 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 501665 501676 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 501677 501678 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 501677 527197 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 527196 527197 . + . intron_id 6 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 527198 527276 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 527198 527276 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 527277 527278 . + . intron_id 8 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 527277 534455 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 534454 534455 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 534456 534512 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 534456 534512 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 534513 534514 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 534513 561394 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 561393 561394 . + . intron_id 8 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 561395 561452 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 561395 561452 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 561453 561454 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 561453 566705 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 566704 566705 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 566706 566725 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 566706 566725 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 566726 566727 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 566726 584424 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 584423 584424 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 584425 584435 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 584425 584435 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 584436 584437 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 584436 602480 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 602479 602480 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 602481 602482 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 602481 602482 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 602483 602484 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 602483 628766 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 628765 628766 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 628767 628776 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 628767 628776 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 628777 628778 . + . intron_id 14 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 628777 629274 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 629273 629274 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 629275 629296 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 629275 629296 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 629297 629298 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 629297 657571 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 657570 657571 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 657572 657583 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 657572 657583 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 657584 657585 . + . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 657584 666657 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 711236 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 711235 711236 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 711237 711256 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 711237 711256 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 711257 711258 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 711257 756874 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 756873 756874 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 756875 756883 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 756875 756883 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 756884 756885 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 756884 868685 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 868684 868685 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 868686 868740 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 868686 868740 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 868741 868742 . + . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 868741 961769 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 961768 961769 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 961770 961786 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 961770 961786 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 961787 961788 . + . intron_id 21 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 961787 982306 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 982305 982306 . + . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 982307 982307 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 982307 982307 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 982308 982309 . + . intron_id 22 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 982308 1011376 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1064120 . + . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1064119 1064120 . + . intron_id 22 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1064121 1064129 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1064121 1064129 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1064130 1064131 . + . intron_id 24 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1064130 1094738 . + . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1094737 1094738 . + . intron_id 23 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1094739 1094747 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1094739 1094747 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1094748 1094749 . + . intron_id 25 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1094748 1112147 . + . intron_id 25 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 24 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 26 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1125835 . + . intron_id 26 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1125834 1125835 . + . intron_id 25 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1125836 1125868 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1125836 1125868 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 12273 1125868 164 + . alignment_id 2 ; Query SPP00000007_1.0 ; Align 12273 4 12 ; Align 79745 9 12 ; Align 154547 13 21 ; Align 287671 21 24 ; Align 324603 30 18 ; Align 342890 37 15 ; Align 501666 43 9 ; Align 527199 47 78 ; Align 534456 73 57 ; Align 561395 92 57 ; Align 566708 112 18 ; Align 584425 118 9 ; Align 628769 123 6 ; Align 629276 126 21 ; Align 657572 133 12 ; Align 711237 137 18 ; Align 756876 144 6 ; Align 868687 147 54 ; Align 961770 165 15 ; Align 1064121 171 9 ; Align 1094739 174 9 ; Align 1125836 177 33 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 158 Query range: 3 -> 194 Target range: 13845 -> 1097740 4 : GlyArgLeuCys >>>> Target Intron 1 >>>> ArgLeuLeuLys > : 12 ||||||||| 3040 bp !:!|||! !||| GlyArgLeuGln++ ++LysLeuGlnLys++ 13846 : GGAAGGCTGCAGgt.........................agAAGTTGCAGAAGgt. : 16912 13 : >>> Target Intron 2 >>>> ProAlaLeuLeuCysGlyAlaLeuAlaAla : 21 17213 bp |||!.!|||! ! ! ||||||! ! !!.! ++ProGlyLeuArgGlyGlyAlaArgArgGly 16913 : ........................agCCTGGTCTTCGAGGAGGAGCCCGAAGAGGC : 34150 22 : ProGlyLeuAla{G} >>>> Target Intron 3 >>>> {ly}ThrMetCy : 29 ||| !! ! !!{|} 5650 bp {||}!:! || ProArgGlyPro{G}++ ++{ly}SerTyrCy 34151 : CCCAGGGGGCCA{G}gt.........................ag{GC}AGCTACTG : 39824 30 : sAlaSer{Ar} >>>> Target Intron 4 >>>> {g}AspAspTrpArg{ : 37 |.!!|||{||} 36167 bp {|}||| !|||!:!{ sThrSer{Ar}++ ++{g}AspPheTrpGln{ 39825 : TACCAGC{CG}gt.........................ag{G}GACTTCTGGCAA{ : 76015 38 : C} >>>> Target Intron 5 >>>> {ys}AlaArgSerMetHis{Gl} : 43 } 25975 bp { }::! !! !!:!!|||{..} V}++ ++{al}SerGlyProLeuHis{Ar}++ 76016 : G}gt.........................ag{TG}AGTGGCCCATTGCAC{AG}gt : 102010 44 : >>>> Target Intron 6 >>>> {u}PheSerAlaLysAspIleAspGlyHi : 52 8019 bp {!} ! ! ! !!!::!!.!.|||!. ++{g}AsnLeuArgLeuGluLeuGlnGlyAr 102011 : .........................ag{A}AATCTCAGATTGGAATTACAGGGCCG : 110054 53 : sMet >>>> Target Intron 7 >>>> ValAsnLeuAspLysTyrArgGl : 61 !||| 36321 bp ! !...:!! !..!|||! !|| gMet++ ++GluGlyMetProSerTyrLeuGl 110055 : TATGgt.........................agGAAGGGATGCCCTCATACCTGGG : 146402 62 : yPheValCysIleValThrAsnValAla{Se} >>>> Target Intron 8 > : 71 |! !!.!|||! !:!! ! !.!|||{||} 24627 bp yCysAlaCysAsnLeuGlyProAlaAla{Se}++ 146403 : GTGTGCTTGCAACCTGGGACCAGCAGCC{AG}gt...................... : 146436 72 : >>> {r}Gln >>>> Target Intron 9 >>>> UnkGlyLysThrGluV : 78 {|}||| 29653 bp |||||| ... ++{r}Gln++ ++ArgGlyLysLeuArgP 146437 : ...ag{C}CAGgt.........................agagggggaaactgaggc : 200733 79 : alAsnTyrThrGlnLeuValAspLeu{Hi} >>>> Target Intron 10 >> : 87 ::: :!!..! !||||||! !{ } 11658 bp roHisArgSerMetArgValAspPro{Gl}++ 200734 : cccacaGGTCAATGAGGGTGGATCCA{GG}gt........................ : 200764 88 : >> {s}AlaArgTyrAlaGluCysGlyLeu{A} >>>> Target Intron 1 : 96 { }|||||| |||||| |||{|} 88602 bp ++{y}AlaArgAlaLeuGluCysArgLeu{A}++ 200765 : ..ag{a}gcacgggctctagagtgcaggctc{a}gt.................... : 212448 97 : 1 >>>> {rg}IleLeuAlaPheProCysAsnGlnPheGlyLysGlnGluPro : 111 {||}! ! ! !!|||! !|||...:!! ||| ! :!!! ! -+{rg}SerSerProPheLeuCysGlyGluProGlyProProGlnGln++ 212449 : ......tg{GG}AGTAGCCCCTTCCTGTGTGGGGAGCCGGGCCCTCCTCAGCAGgt : 301092 112 : >>>> Target Intron 12 >>>> GlySerAsnGluGluIleLysGluPheA : 120 89977 bp ...|||:::... |||.!.|||: +-LysLysAsnLysArgArgLysAsnPheS 301093 : ..........................aaaaaaaaaacaagaggagGAAAAACTTTA : 391096 121 : laAla{Gl} >>>> Target Intron 13 >>>> {y}TyrAsnValLysPh : 127 :! !{ !} 6408 bp {!}:!!!!. !!! || erTyr{Tr}++ ++{p}HisLysPheIlePh 391097 : GTTAC{TG}gt..........................ag{G}CATAAGTTCATTTT : 397525 128 : eAspMetPheSerLysIleCysVal{As} >>>> Target Intron 14 >>> : 136 |.!.:!:!:!..!::::!!|||:!!{ } 1146 bp eGlnLeuTyrGluArgLeuCysIle{Ph}++ 397526 : TCAACTCTATGAGCGTCTTTGTATT{TT}gt......................... : 397556 137 : > {n}GlyAspAsp{A} >>>> Target Intron 15 >>>> {la}HisP : 142 {!}|||...|||{!} 9458 bp {.!}||| ++{e}GlySerAsp{G}++ ++{ly}HisA 397557 : .ag{t}ggctcagat{g}gt..........................ag{GA}CATg : 408174 143 : roLeu{Tr} >>>> Target Intron 16 >>>> {p}LysTrpMetLys{I : 149 :::{||} 54448 bp {|}... ::: {| laIle{Tr}++ ++{p}SerHisValTrp{I 408175 : caata{tg}gt..........................ag{g}agtcatgtatgg{a : 462643 150 : l} >>>> Target Intron 17 >>>> {e}GlnProLys{G} >>>> Ta : 153 |} 39931 bp {|}! |||:!!{|} l}++ ++{e}LeuProGln{G}++ 462644 : t}gt..........................ag{C}CTCCCCCAG{G}gt....... : 502589 154 : rget Intron 18 >>>> {ly}Lys >>>> Target Intron 19 >>>> : 155 85940 bp {||}:!! 12928 bp ++{ly}Gln++ 502590 : ...................ag{GG}CAGgt.......................... : 588534 156 : GlyIleLeuGlyAsnAla >>>> Target Intron 20 >>>> >>>> : 161 ||||||... 65180 bp ++GlyIlePheProIleGln++ ++++ 588535 : aggggatcttcccaatccaggt..........................aggt.... : 666660 162 : Target Intron 21 >>>> IleLysTrpAsnPhe >>>> Target Int : 166 119566 bp :::::!|||||| 77534 ++ValArgTrpAsnGlu-+ 666661 : ......................aggtgCGATGGAACGAGtt............... : 786241 167 : ron 22 >>>> ThrLysPheLeuIle{As} >>>> Target Intron 23 : 171 bp ! !|||||| ! !{..} 7634 bp ++IleLysPheAsnSer{Se}++ 786242 : ...........agATAAAATTCAATTCC{AG}gt...................... : 863792 172 : >>>> {p}LysAsnGlyCysValValLysArgTyr{G} >>>> Target Int : 181 {!}!:!.!.|||||| !|||! ! !!{|} 121792 ++{r}ArgGlnGlyCysAsnValThrSerAsn{G}++ 863793 : ....ag{C}AGGCAAGGGTGCAACGTGACTTCCAAT{G}gt............... : 871455 182 : ron 24 >>>> {ly}ProMetGluGluProLeuValIleGlu{Ly} >>>> T : 191 bp {||}! !:!:! !.!.|||! ! !||||||{::} ++{ly}GlnLeuGlyAsnProArgArgIleGlu{Ar}++ 871456 : ...........ag{GC}CAGTTAGGGAATCCACGGAGGATCgaa{cg}gt...... : 993278 192 : arget Intron 25 >>>> {s}AspLeuPro : 194 104455 bp {!}|||:::||| ++{g}AspValPro 993279 : ....................ag{g}gatgttccc : 1097740 vulgar: SPP00000007_1.0 3 194 . NIVO01030317.1:subseq(555646,1182752) 13845 1097740 + 158 M 4 12 5 0 2 I 0 3036 3 0 2 M 4 12 5 0 2 I 0 17209 3 0 2 M 14 42 S 0 1 5 0 2 I 0 5646 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 36163 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 25971 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 8015 3 0 2 S 1 1 M 10 30 5 0 2 I 0 36317 3 0 2 M 17 51 S 0 2 5 0 2 I 0 24623 3 0 2 S 1 1 M 1 3 5 0 2 I 0 29649 3 0 2 M 14 42 S 0 2 5 0 2 I 0 11654 3 0 2 S 1 1 M 8 24 S 0 1 5 0 2 I 0 88598 3 0 2 S 1 2 M 14 42 5 0 2 I 0 89973 3 0 2 M 11 33 S 0 2 5 0 2 I 0 6404 3 0 2 S 1 1 M 13 39 S 0 2 5 0 2 I 0 1142 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 9454 3 0 2 S 1 2 M 3 9 S 0 2 5 0 2 I 0 54444 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 39927 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 85936 3 0 2 S 1 2 M 1 3 5 0 2 I 0 12924 3 0 2 M 6 18 5 0 2 I 0 65176 3 0 2 5 0 2 I 0 119562 3 0 2 M 5 15 5 0 2 I 0 77530 3 0 2 M 5 15 S 0 2 5 0 2 I 0 7630 3 0 2 S 1 1 M 9 27 S 0 1 5 0 2 I 0 121788 3 0 2 S 1 2 M 9 27 S 0 2 5 0 2 I 0 104451 3 0 2 S 1 1 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 13846 1097740 158 + . gene_id 3 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 13846 13857 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 13846 13857 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 13858 13859 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 13858 16897 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 16896 16897 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 16898 16909 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 16898 16909 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 16910 16911 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 16910 34122 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 34121 34122 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 34123 34165 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 34123 34165 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 34166 34167 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 34166 39815 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 39814 39815 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 39816 39834 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 39816 39834 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 39835 39836 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 39835 76001 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 76000 76001 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 76002 76015 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 76002 76015 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 76016 76017 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 76016 101990 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 101989 101990 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 101991 102009 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 101991 102009 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 102010 102011 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 102010 110028 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 110027 110028 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 110029 110059 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 110029 110059 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 110060 110061 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 110060 146380 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146379 146380 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146381 146433 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146381 146433 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146434 146435 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146434 171060 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 200717 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 200716 200717 . + . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 200718 200761 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 200718 200761 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 200762 200763 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 200762 212419 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 212418 212419 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 212420 212445 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 212420 212445 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 212446 212447 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 212446 301047 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 301046 301047 . + . intron_id 10 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 301048 301091 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 301048 301091 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 301092 301093 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 301092 391068 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 391067 391068 . + . intron_id 11 ; splice_site "aa" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 391069 391103 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 391069 391103 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 391104 391105 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 391104 397511 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 397510 397511 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 397512 397553 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 397512 397553 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 397554 397555 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 397554 398699 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 398698 398699 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 398700 398710 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 398700 398710 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 398711 398712 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 398711 408168 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 408167 408168 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 408169 408181 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 408169 408181 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 408182 408183 . + . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 408182 462629 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462644 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462644 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462645 462646 . + . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462645 502575 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 502574 502575 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 502576 502586 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 502576 502586 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 502587 502588 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 502587 588526 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 588525 588526 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 588527 588531 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 588527 588531 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 588532 588533 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 588532 601459 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 666657 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 786223 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 786222 786223 . + . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 786224 786238 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 786224 786238 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 786239 786240 . + . intron_id 22 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 786239 863772 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 871423 . + . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 871422 871423 . + . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 871424 871452 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 871424 871452 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 871453 871454 . + . intron_id 24 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 871453 993244 . + . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 993243 993244 . + . intron_id 23 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 993245 993275 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 993245 993275 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 993276 993277 . + . intron_id 25 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 993276 1097730 . + . intron_id 25 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1097729 1097730 . + . intron_id 24 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1097731 1097740 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1097731 1097740 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 13846 1097740 158 + . alignment_id 3 ; Query SPP00000007_1.0 ; Align 13846 4 12 ; Align 16898 8 12 ; Align 34123 12 42 ; Align 39818 27 15 ; Align 76003 33 12 ; Align 101993 38 15 ; Align 110030 44 30 ; Align 146381 54 51 ; Align 171062 72 3 ; Align 200718 73 42 ; Align 212421 88 24 ; Align 301050 97 42 ; Align 391069 111 33 ; Align 397513 123 39 ; Align 398701 137 9 ; Align 408171 141 9 ; Align 462631 145 12 ; Align 502577 150 9 ; Align 588529 154 3 ; Align 601460 155 18 ; Align 786224 161 15 ; Align 863773 166 15 ; Align 871425 172 27 ; Align 993247 182 27 ; Align 1097732 192 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 157 Query range: 6 -> 194 Target range: 780 -> 1109951 7 : CysArgLeuLeuLysProAlaLeuLeuCysGly{Al} >>>> Target Intro : 18 ||| !||| ! ||| !||||||||||||{ } 26493 bp CysValLeuAsnProProTrpLeuLeuCysGly{Gl}++ 781 : TGTGTTTTGAACCCCCCGTGGCTGCTGTGTGGG{CA}gt................. : 818 19 : n 1 >>>> {a}LeuAlaAlaPro{G} >>>> Target Intron 2 >>>> : 23 {!}||||||!.!|||{|} 64503 bp ++{n}LeuAlaGlyPro{G}+- + 819 : ........ag{G}CTGGCAGGGCCA{G}ga.........................a : 91824 24 : {ly}LeuAlaGlyThrMetCysAlaSer{Ar} >>>> Target Intron 3 : 32 {||}||| !!|||.!! ||| !..!{||} 37179 bp +{ly}LeuProGlyAlaSerCysLysGln{Ar}-+ 91825 : g{GG}CTTCCAGGGGCCTCATGCAAACAA{CG}ct..................... : 91856 33 : >>>> {g}AspAspTrpArgCysAlaArgSerMet >>>> Target Intron : 42 {|}! !.!.||| ! ||| !!!!..! 17520 bp ++{g}AlaGlnTrpAlaLeuAlaPheThrGln++ 91857 : ....ag{G}GCCCAGTGGGCTCTGGCCTTCACCCAGgt.................. : 129063 43 : 4 >>>> HisGluPheSerAlaLys >>>> Target Intron 5 >>>> : 48 ||| !!::!:!! 22185 bp ++AlaGluGluProSerGln++ ++ 129064 : .......agGCAGAGGAACCCAGCCAGgt.........................ag : 168782 49 : AspIleAspGlyHisMetValAsnLeuAspLysTyr{A} >>>> Target Int : 60 ..!:!:||||||!....!:!!..!:!!|||:!! { } 103940 SerValAspGlyArgGlnIleArgValAspGlnAla{G}+- 168783 : TCTGTGGATGGGCGGCAGATCCGCGTGGACCAGGCA{G}gc............... : 168823 61 : ron 6 >>>> {rg}{G} >>>> Target Intron 7 >>>> {ly}PheV : 63 bp {!!}{|} 17103 bp {||}||| ++{ly}{G}++ ++{ly}PheP 168824 : ..........ag{GA}{G}gt.........................ag{GA}TTCC : 289872 64 : alCysIleValThrAsnValAlaSerGlnUnk >>>> Target Intron 8 > : 74 !..!:!::!!..!:!! !||||||.!. 94688 bp roAlaValMetValAspArgAlaSerAspGln++ 289873 : CAGCTGTGATGGTAGACAGGGCCTCAGACCAGgt...................... : 289907 75 : >>> GlyLysThrGluValAsnTyrThrGln >>>> Target Intron 9 > : 83 ||| !..!!.! !::|||:!! 60458 bp ++GlyProGlnArgAlaLeuTrpThrGlu++ 289908 : ...agGGTCCCCAGAGAGCCCTGTGGACAGAGgt...................... : 384622 84 : >>> LeuValAspLeuHisAlaArg >>>> Target Intron 10 >>>> : 90 :!!:!! !! !|||:!!||| 121607 bp ++IleLeuThrArgHisSerArg++ ++ 384623 : ...agATCTTAACTCGCCACTCTCGGgt..........................ag : 566704 91 : TyrAlaGluCysGlyLeu{Ar} >>>> Target Intron 11 >>>> {g}I : 97 ! |||.!.|||.!!!!!{||} 136409 bp {|}| GluAlaHisCysSerPhe{Ar}++ +-{g}I 566705 : gAAGCGCATTGCAGCTTC{AG}gt..........................ac{A}A : 703136 98 : leLeuAlaPheProCysAsnGlnPhe{G} >>>> Target Intron 12 >>> : 106 || ...|||||||||...... {|} 14742 bp le***GlyPheProCysGlySerAsp{G}++ 703137 : tttaaggcttcccttgtggctcagac{g}gt......................... : 703166 107 : > {ly}{L} >>>> Target Intron 13 >>>> {ys}GlnGluProGly : 111 {||}{!} 39272 bp {:!}...||||||||| ++{ly}{A}++ ++{rg}MetGluProGly 703167 : .ag{GA}{A}gt..........................ag{gg}atggaacccggg : 757192 112 : SerAsnGluGluIleLysGluPhe{A} >>>> Target Intron 14 >>>> : 120 |||:::||| {|} 57956 bp SerSerGluThrProAlaLeuAla{A}++ + 757193 : tcttctgagactcctgctttggca{g}gt..........................a : 815174 121 : {la}Ala >>>> Target Intron 15 >>>> GlyTyrAsn >>>> Ta : 125 {||} ! 118940 bp |||!:!||| +{la}Leu++ ++GlyPheAsn++ 815175 : g{CG}TTGgt..........................agGGCTTCAACgt....... : 934132 126 : rget Intron 16 >>>> {V} >>>> Target Intron 17 >>>> {a : 125 48177 bp {|} 791 bp {| ++{V}++ -+{a 934133 : ...................ag{g}gt..........................tg{T : 983099 126 : l}LysPheAspMetPheSerLysIleCysValAsn >>>> Target Intron : 137 |} ! |||||||||! ! :!:|||...||| 28243 bp l}CysSerAspMetPheLeuProValCysAlaAsn++ 983100 : G}TGCTCGGACATGTTTTTGCCTGTGTGTgcgaatgt................... : 983136 138 : 18 >>>> >>>> Target Intron 19 >>>> GlyAspAsp >>>> T : 140 52744 bp |||...::: ++++ ++GlySerAsn++ 983137 : .......aggt..........................agggatcaaacgt...... : 1064132 141 : arget Intron 20 >>>> AlaHisProLeuTrpLysTrpMet{L} >>>> : 148 3082 bp !.!||| !:!!||| ||| { } -+GlyHisArgValTrpValTrpGlu{G}+- 1064133 : ....................ggGGGCACAGAGTCTGGGTCTGGGAA{G}gc..... : 1067239 149 : Target Intron 21 >>>> {ys}IleGlnProLysGlyLysGlyIleLeu : 158 3021 bp { !}|||||||||:::... ...::: ++{ly}IleGlnProArgSerProAlaLeuGln++ 1067240 : .....................ag{gg}attcaacccaggtctcctgcattgcaggt : 1070287 159 : >>>> Target Intron 22 >>>> GlyAsnAlaIleLysTrpAsnPheThrL : 167 16651 bp ||||||! |||:!!|||.!!! ++PheTyrAlaIleIleTrpAspPheAlaA 1070288 : ..........................agttttatgcTATAATTTGGGATTTTGCAA : 1086965 168 : ysPheLeuIleAspLysAsnGlyCysVal{V} >>>> Target Intron 23 : 177 !.|||:!!:!! ! ..! !! ! !{|} 22903 bp snPheMetLeuLysPheGlyTrpAsnArg{V}++ 1086966 : ATTTTATGCTAAAATTTGGCTGGAACAGG{G}gt...................... : 1086998 178 : >>>> {al}LysArgTyrGlyProMetGluGluProLeuValIleGluLysAspL : 193 {||}!.. !||| !|||! :!!:!!|||! !!.!:!: ! ! | ++{al}SerSerTyrHisProArgLysLysProCysAlaLeuTrpLeuProL 1086999 : ....ag{TG}AGCTCTTATCACCCGAGAAAGAAGCCTTGTGCCCTGTGGTTGccac : 1109946 194 : euPro : 194 ||||| euPro 1109947 : tgccc : 1109951 vulgar: SPP00000007_1.0 6 194 . NIVO01030317.1:subseq(555646,1182752) 780 1109951 + 157 M 11 33 S 0 2 5 0 2 I 0 26489 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 64499 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 37175 3 0 2 S 1 1 M 9 27 5 0 2 I 0 17516 3 0 2 M 6 18 5 0 2 I 0 22181 3 0 2 M 12 36 S 0 1 5 0 2 I 0 103936 3 0 2 S 1 3 5 0 2 I 0 17099 3 0 2 S 1 2 M 12 36 5 0 2 I 0 94684 3 0 2 M 9 27 5 0 2 I 0 60454 3 0 2 M 7 21 5 0 2 I 0 121603 3 0 2 M 6 18 S 0 2 5 0 2 I 0 136405 3 0 2 S 1 1 M 9 27 S 0 1 5 0 2 I 0 14738 3 0 2 S 1 3 5 0 2 I 0 39268 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 57952 3 0 2 S 1 2 M 1 3 5 0 2 I 0 118936 3 0 2 M 3 9 5 0 2 I 0 48173 3 0 2 S 0 1 5 0 2 I 0 787 3 0 2 S 1 2 M 11 33 5 0 2 I 0 28239 3 0 2 5 0 2 I 0 52740 3 0 2 M 3 9 5 0 2 I 0 3078 3 0 2 M 8 24 S 0 1 5 0 2 I 0 3017 3 0 2 S 1 2 M 9 27 5 0 2 I 0 16647 3 0 2 M 19 57 S 0 1 5 0 2 I 0 22899 3 0 2 S 1 2 M 17 51 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 781 1109951 157 + . gene_id 4 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 781 815 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 781 815 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 816 817 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 816 27308 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 27307 27308 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 27309 27322 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 27309 27322 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 27323 27324 . + . intron_id 2 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 27323 91825 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 91824 91825 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 91826 91853 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 91826 91853 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 91854 91855 . + . intron_id 3 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 91854 129032 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 129031 129032 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 129033 129060 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 129033 129060 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 129061 129062 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 129061 146580 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146579 146580 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146581 146598 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146581 146598 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146599 146600 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146599 168783 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 168782 168783 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 168784 168820 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 168784 168820 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 168821 168822 . + . intron_id 6 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 168821 272760 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 272759 272760 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 272761 272763 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 272761 272763 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 272764 272765 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 272764 289866 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 289865 289866 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 289867 289904 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 289867 289904 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 289905 289906 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 289905 384592 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 384591 384592 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 384593 384619 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 384593 384619 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 384620 384621 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 384620 445077 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 445076 445077 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 445078 445098 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 445078 445098 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 445099 445100 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 445099 566705 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 566704 566705 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 566706 566725 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 566706 566725 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 566726 566727 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 566726 703134 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 703133 703134 . + . intron_id 10 ; splice_site "AC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 703135 703163 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 703135 703163 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 703164 703165 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 703164 717905 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 717904 717905 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 717906 717908 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 717906 717908 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 717909 717910 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 717909 757180 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 757179 757180 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 757181 757219 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 757181 757219 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 757220 757221 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 757220 815175 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 815174 815175 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 815176 815180 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 815176 815180 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 815181 815182 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 815181 934120 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 934119 934120 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 934121 934129 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 934121 934129 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 934130 934131 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 934130 982306 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 982305 982306 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 982307 982307 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 982307 982307 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 982308 982309 . + . intron_id 17 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 982308 983098 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 983097 983098 . + . intron_id 16 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 983099 983133 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 983099 983133 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 983134 983135 . + . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 983134 1011376 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1064120 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1064119 1064120 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1064121 1064129 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1064121 1064129 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1064130 1064131 . + . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1064130 1067211 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1067210 1067211 . + . intron_id 19 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1067212 1067236 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1067212 1067236 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1067237 1067238 . + . intron_id 21 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1067237 1070257 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1070256 1070257 . + . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1070258 1070286 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1070258 1070286 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1070287 1070288 . + . intron_id 22 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1070287 1086937 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1086936 1086937 . + . intron_id 21 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1086938 1086995 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1086938 1086995 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1086996 1086997 . + . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1086996 1109898 . + . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1109897 1109898 . + . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1109899 1109951 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1109899 1109951 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 781 1109951 157 + . alignment_id 4 ; Query SPP00000007_1.0 ; Align 781 7 33 ; Align 27310 19 12 ; Align 91828 24 24 ; Align 129034 33 27 ; Align 146581 42 18 ; Align 168784 48 36 ; Align 289869 62 36 ; Align 384593 74 27 ; Align 445078 83 21 ; Align 566706 90 18 ; Align 703136 97 27 ; Align 757183 108 36 ; Align 815178 121 3 ; Align 934121 122 9 ; Align 983101 126 33 ; Align 1064121 137 9 ; Align 1067212 140 24 ; Align 1070260 149 27 ; Align 1086938 158 57 ; Align 1109901 178 51 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 143 Query range: 0 -> 190 Target range: 16883 -> 1095949 1 : MetSerLeuGlyArgLeuCysArg{Le} >>>> Target Intron 1 >>>> : 9 :!! !!|||.!!||| !||||||{ } 37789 bp LeuProLeuSerArgSerCysArg{Ar}++ + 16884 : TTGCCCCTGAGCAGAAGTTGCAGA{AG}gt.........................a : 54697 10 : {u}LeuLysProAlaLeuLeuCysGlyAlaLeuAlaAla{Pr} >>>> Targe : 22 {!}! !::! !!.!!|||:!!|||||| ! !!.!!.!{ } 9 +{g}ProArgSerThrLeuIleCysGlyHisThrGlyVal{Ar}++ 54698 : g{G}CCACGATCCACACTGATCTGTGGGCACACAGGTGTC{AG}gt.......... : 54740 23 : t Intron 2 >>>> {o}GlyLeuAlaGlyThrMetCysAlaSerArgAspAsp : 34 8745 bp {!}..!:!!.!!..! !..!..!||| !||| ! ++{g}SerValThrSerCysGlnAlaAlaHisArg***Pro 54741 : ...............ag{G}TCAGTGACTTCTTGTCAGGCCGCTCACCGCTGACCC : 153517 35 : TrpArgCys{Al} >>>> Target Intron 3 >>>> {a}Arg >>>> T : 40 |||||||||{!.} 17530 bp {!}!:! TrpArgCys{Va}++ ++{l}Gln++ 153518 : TGGCGCTGC{GT}gt.........................ag{C}CAGgt...... : 171067 41 : arget Intron 4 >>>> SerMetHisGluPhe{Se} >>>> Target In : 45 45425 bp |||..!!!.|||!::{!!} 570 ++SerGlnGlnGluTrp{Th}++ 171068 : ...................agAGCCAGCAGGAGTGG{AC}gt.............. : 216509 46 : tron 5 >>>> {r}AlaLysAspIleAsp >>>> Target Intron 6 >> : 51 bp {!}|||:!!|||:!! ! 7503 bp ++{r}AlaGluAspValPro++ 216510 : ...........ag{g}gCTGAGGATGTTCCTgt....................... : 217095 52 : >> GlyHisMetValAsnLeuAspLysTyrArgGlyPhe{V} >>>> Target : 63 |||||| !|||::!||| ! ! !||| {|} 11 -+GlyHisGlyValSerLeuArgGlyGlnLeuGlyGlu{V}++ 217096 : ..ggGGACACGGGGTCTCCCTCCGAGGCCAGCTAGGGGAA{G}gt........... : 224635 64 : Intron 7 >>>> {al}CysIleValThr{A} >>>> Target Intron : 68 625 bp {||}|||:!!|||! !{|} 11772 bp ++{al}CysLeuValIle{A}++ 224636 : ..............ag{TG}TGTCTTGTGATA{A}gt................... : 236275 69 : 8 >>>> {sn}ValAlaSerGlnUnk{G} >>>> Target Intron 9 >>> : 74 {||}:!!||||||..! {|} 9822 bp ++{sn}IleAlaSerSerLeu{G}++ 236276 : ......ag{AC}ATCGCCTCCTCATTA{G}gt........................ : 248065 75 : > {ly}LysThrGluValAsnTyrThrGln >>>> Target Intron 10 > : 83 {||} ! !!...|||!:!:!!.!!||| 20150 bp ++{ly}TrpProArgValSerHisAlaGln++ 248066 : .ag{GA}TGGCCCCGTGTCAGCCATGCCCAGgt....................... : 257913 84 : >>> LeuValAspLeuHisAlaArgTyrAla{G} >>>> Target Intron : 92 !!!!.!|||||| !!:!!..! ! ! !{!} 41094 bp ++PheAlaAspLeuAspSerGluLysGlu{G}++ 257914 : ...agTTCGCAGACTTGGACTCAGAGAAAGAA{G}gt................... : 278091 93 : 11 >>>> {lu}CysGlyLeuArgIleLeu{Al} >>>> Target Intron : 99 { !}||| !!:!!|||:!!|||{::} 16619 bp ++{ly}CysArgValArgValLeu{Se}++ 278092 : .......ag{GA}TGCAGGGTGAGGGTCTTA{AG}gt................... : 319207 100 : 12 >>>> {a}PheProCysAsnGln{Ph} >>>> Target Intron 13 > : 105 {!} !||||||..!! {!:} 48425 bp ++{r}ArgProCysGlyPro{Tr}++ 319208 : .......ag{c}cgCCCCTGTGGCCCC{TG}gt....................... : 335844 106 : >>> {e}GlyLysGlnGluProGlySerAsn >>>> Target Intron 14 : 114 {:}||| ::: !||||||.!!... 14012 bp ++{p}GlyHisGluGlyProGlyGlyGln++ 335845 : ...ag{g}ggacacgaggGGCCTGGAGGCCaggt...................... : 384294 115 : >>>> Glu{G} >>>> Target Intron 15 >>>> {lu}Ile{Ly} > : 117 |||{.} 64322 bp {.!}:::{! } ++Glu{A}++ ++{rg}Val{Me}++ 384295 : ....aggaa{a}gt..........................ag{ga}gtc{at}gt. : 462639 118 : >>> Target Intron 16 >>>> {s}GluPheAla{Al} >>>> Target : 121 39028 bp {!}||||||:::{::} 36 ++{t}GluPheSer{Se}++ 462640 : .........................ag{g}gaattttcc{ag}gt........... : 501679 122 : Intron 17 >>>> {a}GlyTyrAsnValLysPheAspMet{P} >>>> Ta : 130 529 bp {!}|||:::::: !!:!|||..!:!:{ } ++{r}GlyPheSerSerArgPheSerLeu{A}++ 501680 : ...............ag{t}ggattcTCATCGAGATTTTCTTTA{C}gt....... : 538234 131 : rget Intron 18 >>>> {he}SerLysIleCysValAsnGlyAspAspAlaH : 141 7983 bp { !}||| ! :!!|||!.!:!!|||! !... | ++{rg}SerHisLeuCysAlaHisGlyAlaGlnLeuH 538235 : ...................ag{gc}tcGCATCTCTGCGCCCACGGGGCCCagctcc : 546247 142 : isProLeuTrpLysTrpMetLysIleGlnProLysGlyLys{G} >>>> Targe : 155 || ::: ::::!! ... ||| {|} 5 isPheHisPheLeuSerValGluSerSerSerProGlyGly{G}+- 546248 : actttcactttctctctgtgGAGTccagctctcctggagga{g}gc.......... : 546292 156 : t Intron 19 >>>> {ly}IleLeuGlyAsnAla{I} >>>> Target In : 161 5170 bp {||} ||| ||| {:} 52123 ++{ly}AspLeuProAsnPro{V}++ 546293 : ................ag{gg}gatcttcccaatcca{g}gt.............. : 601480 162 : tron 20 >>>> {le}LysTrpAsnPheThrLys{P} >>>> Target Int : 168 bp {!:}||||||.!. !! !|||{ } 61045 ++{al}LysTrpGlnValGluLys{S}++ 601481 : ............ag{TG}AAGTGGCAAGTTGAGAAA{A}gt............... : 653624 169 : ron 21 >>>> {he}LeuIleAsp{L} >>>> Target Intron 22 >>> : 172 bp { !}||||||..!{|} 79140 bp ++{er}LeuIleSer{L}++ 653625 : ...........ag{GT}TTAATCAGT{A}gt......................... : 714681 173 : > {ys}Asn >>>> Target Intron 23 >>>> GlyCysValValLysA : 179 {||}.!. 19693 bp |||||||||:!! ! ++{ys}Gln+- +-GlyCysValIleLeuH 714682 : .ag{AG}CAGga..........................acggCTGCGTAATACTCC : 813532 180 : rgTyr >>>> Target Intron 24 >>>> >>>> Target Intron : 181 .!!:: 197839 bp 84543 bp isTrp++ ++++ 813533 : ACTGGgt..........................aggt................... : 1011379 182 : 25 >>>> GlyProMetGluGluProLeuValIleGlu : 190 ||||||:!!.!. !||||||:!! !||| ++GlyProValHisProProLeuLeuProGlu 1011380 : .......agGGTCCTGTGCACCCGCCACTTCTCCCAGAG : 1095949 vulgar: SPP00000007_1.0 0 190 . NIVO01030317.1:subseq(555646,1182752) 16883 1095949 + 143 M 8 24 S 0 2 5 0 2 I 0 37785 3 0 2 S 1 1 M 12 36 S 0 2 5 0 2 I 0 98741 3 0 2 S 1 1 M 15 45 S 0 2 5 0 2 I 0 17526 3 0 2 S 1 1 M 1 3 5 0 2 I 0 45421 3 0 2 M 5 15 S 0 2 5 0 2 I 0 566 3 0 2 S 1 1 M 5 15 5 0 2 I 0 7499 3 0 2 M 12 36 S 0 1 5 0 2 I 0 11621 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 11768 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 9818 3 0 2 S 1 2 M 8 24 5 0 2 I 0 20146 3 0 2 M 9 27 S 0 1 5 0 2 I 0 41090 3 0 2 S 1 2 M 6 18 S 0 2 5 0 2 I 0 16615 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 48421 3 0 2 S 1 1 M 8 24 5 0 2 I 0 14008 3 0 2 M 1 3 S 0 1 5 0 2 I 0 64318 3 0 2 S 1 2 M 1 3 S 0 2 5 0 2 I 0 39024 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 36525 3 0 2 S 1 1 M 8 24 S 0 1 5 0 2 I 0 7979 3 0 2 S 1 2 M 24 72 S 0 1 5 0 2 I 0 55166 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 52119 3 0 2 S 1 2 M 6 18 S 0 1 5 0 2 I 0 61041 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 79136 3 0 2 S 1 2 M 1 3 5 0 2 I 0 19689 3 0 2 M 7 21 5 0 2 I 0 197835 3 0 2 5 0 2 I 0 84539 3 0 2 M 10 30 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 16884 1095949 143 + . gene_id 5 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 16884 16909 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 16884 16909 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 16910 16911 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 16910 54698 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 54697 54698 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 54699 54737 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 54699 54737 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 54738 54739 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 54738 153482 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 153481 153482 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 153483 153530 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 153483 153530 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 153531 153532 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 153531 171060 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 216489 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 216488 216489 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 216490 216506 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 216490 216506 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 216507 216508 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 216507 217076 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 217075 217076 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 217077 217092 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 217077 217092 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 217093 217094 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 217093 224595 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 224594 224595 . + . intron_id 5 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 224596 224632 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 224596 224632 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 224633 224634 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 224633 236257 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 236256 236257 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 236258 236272 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 236258 236272 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 236273 236274 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 236273 248044 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 248043 248044 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 248045 248062 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 248045 248062 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 248063 248064 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 248063 257884 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 257883 257884 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 257885 257910 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 257885 257910 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 257911 257912 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 257911 278060 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 278059 278060 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 278061 278088 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 278061 278088 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 278089 278090 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 278089 319182 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 319181 319182 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 319183 319204 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 319183 319204 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 319205 319206 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 319205 335823 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 335822 335823 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 335824 335841 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 335824 335841 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 335842 335843 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 335842 384266 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 384265 384266 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 384267 384291 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 384267 384291 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 384292 384293 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 384292 398303 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 398302 398303 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 398304 398307 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 398304 398307 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 398308 398309 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 398308 462629 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462636 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462636 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462637 462638 . + . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462637 501664 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 501663 501664 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 501665 501676 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 501665 501676 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 501677 501678 . + . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 501677 538205 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 538204 538205 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 538206 538231 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 538206 538231 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 538232 538233 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 538232 546214 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546213 546214 . + . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546215 546289 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546215 546289 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546290 546291 . + . intron_id 19 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546290 601459 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 653600 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 653599 653600 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 653601 653621 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 653601 653621 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 653622 653623 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 653622 714666 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 714665 714666 . + . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 714667 714678 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 714667 714678 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 714679 714680 . + . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 714679 793818 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 793817 793818 . + . intron_id 21 ; splice_site "aG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 793819 793823 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 793819 793823 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 793824 793825 . + . intron_id 23 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 793824 813516 . + . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 813515 813516 . + . intron_id 22 ; splice_site "ac" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 813517 813537 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 813517 813537 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 813538 813539 . + . intron_id 24 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 813538 1011376 . + . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 23 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 25 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1095919 . + . intron_id 25 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1095918 1095919 . + . intron_id 24 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1095920 1095949 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1095920 1095949 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 16884 1095949 143 + . alignment_id 5 ; Query SPP00000007_1.0 ; Align 16884 1 24 ; Align 54700 10 36 ; Align 153484 23 45 ; Align 171062 39 3 ; Align 216490 40 15 ; Align 217078 46 15 ; Align 224596 51 36 ; Align 236260 64 12 ; Align 248047 69 15 ; Align 257887 75 24 ; Align 278061 83 27 ; Align 319185 93 18 ; Align 335825 100 15 ; Align 384268 106 24 ; Align 398304 114 3 ; Align 462632 116 3 ; Align 501666 118 9 ; Align 538207 122 24 ; Align 546217 131 72 ; Align 601462 156 15 ; Align 653603 162 18 ; Align 714669 169 9 ; Align 793821 173 3 ; Align 813517 174 21 ; Align 1095920 181 30 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 142 Query range: 3 -> 195 Target range: 58041 -> 1172216 4 : GlyArgLeuCysArgLeu{Le} >>>> Target Intron 1 >>>> {u}Ly : 11 ||| !!|||||| !|||{ } 21348 bp {!}:! GlyTrpLeuCysAlaLeu{Ar}++ ++{g}Gl 58042 : GGCTGGCTGTGTGCTCTC{AG}gt.........................ag{g}GA : 79411 12 : sProAlaLeuLeuCysGly{Al} >>>> Target Intron 2 >>>> {a}L : 19 ! !||||||!!!||| !!{ } 38892 bp {!}| uGlyAlaLeuPheCysCys{Ar}+- ++{g}L 79412 : AGGAGCCCTGTTCTGCTGC{AG}gc.........................ag{G}C : 118327 20 : euAlaAla >>>> Target Intron 3 >>>> Pro{G} >>>> Target : 23 |||||||| 52725 bp |||{!} 14 euAlaAla++ ++Pro{A}++ 118328 : TAGCAGCGgt.........................agCCA{G}gt........... : 171067 24 : Intron 4 >>>> {ly}LeuAlaGlyThrMetCys{Al} >>>> Target : 30 05 bp {.!}! ! !|||! !||||||{::} 208 ++{la}ProLysGlyArgMetCys{Se}++ 171068 : ..............ag{CC}CCCAAAGGCAGGATGTGC{AG}gt............ : 172494 31 : Intron 5 >>>> {a}SerArgAspAspTrpArgCysAlaArg{S} >>>> T : 40 17 bp {!}!!!|||||| !||| !!...!.!|||{.} ++{r}ThrArgAspPheTrpGlyAlaGlyArg{G}++ 172495 : .............ag{C}ACGAGGGACTTCTGGGGGGCAGGCAGA{G}gt...... : 193340 41 : arget Intron 6 >>>> {er}Met >>>> Target Intron 7 >>>> : 42 327 bp {!!}:!: 65087 bp ++{ly}Leu++ + 193341 : ...................ag{GC}CTAgt.........................a : 258755 43 : HisGluPheSerAlaLysAspIleAspGlyHisMet{Va} >>>> Target I : 54 .!.|||! !|||! !:!!|||:!! ! !||| { } 9324 +GluGluCysSerAspGlnAspValProLeuHisPro{Cy}++ 258756 : gGAAGAATGCAGTGATCAAGATGTCCCTCTACATCCA{TG}gt............. : 258797 55 : ntron 8 >>>> {l}AsnLeuAspLysTyrArgGlyPheValCysIleValThr : 67 8 bp {!}.!. !:!!||| ! !||||||:!!||| !||| ! ++{s}GluAlaAsnLysLeuTyrGlyPheLeuCysArgValTrp 258798 : ............ag{C}GAGGCAAACAAGCTTTACGGTTTCTTGTGTCGCGTGTGG : 352080 68 : AsnValAlaSer >>>> Target Intron 9 >>>> Gln{U} >>>> Ta : 73 !!.!.!!.!!!! 63029 bp |||{ } LysAlaGlyArg++ ++Gln{G}++ 352081 : AAAGCAGGTAGGgt.........................agcaa{g}gt....... : 415130 74 : rget Intron 10 >>>> {nk}GlyLysThrGluValAsnTyrThrGlnLeuV : 84 37692 bp { }|||::: ||| !... ! ||| !:!!: ++{ly}GlyArgGluGluProGlyGluThrGlyValL 415131 : ...................ag{ga}ggcagagaagagCCGGGGGAAACCGGGGTCC : 452852 85 : alAspLeuHisAlaArgTyrAlaGlu{C} >>>> Target Intron 11 >>> : 93 !!...:!! ! ! !|||!::!.!.!.{|} 30734 bp euSerIleLysAspArgTrpGlyHis{C}+- 452853 : TGTCGATAAAAGACCGCTGGGGACAC{T}gg......................... : 452882 94 : > {ys}GlyLeuArgIleLeuAlaPheProCysAsnGlnPheGlyLysGlnGluP : 110 {||}||| !||| !|||! ! ! !::!::!.!.!.!!:!.!.|||| ++{ys}GlyAlaArgGluAlaAlaSerLeuThrSerArgLeuAlaArgAsnGluP 452883 : .ag{GC}GGCGCCCGGGAGGCGGCCTCGCTCACCTCCAGGCTGGCCAGGAATGAGC : 483664 111 : ro >>>> Target Intron 12 >>>> GlySerAsnGluGluIleLysGlu : 118 || 100907 bp ||||||||| ...::: ::: ro+- ++GlySerAsnAlaHisLeuLeuGln 483665 : CGgc..........................agggatcgaacgctcatctcttgcaa : 584595 119 : PheAla >>>> Target Intron 13 >>>> AlaGlyTyrAsnValLys : 127 ::: 16856 bp ... ::: :::::: TrpGln++ ++GlyIlePheProIleGln++ 584596 : tggcaggt..........................aggggatcttcccaatccaggt : 601478 128 : >>>> Target Intron 14 >>>> {Ph} >>>> Target Intron 15 : 127 1003 bp { } 77760 bp ++{Gl}++ 601479 : ..........................ag{gg}gt...................... : 602485 128 : >>>> {e}AspMetPheSerLysIleCysValAsnGlyAspAspAlaHisProLe : 143 { } ::: ||| :::|||! !.!.||| ! ! !|||||||| ++{y}HisValGlySer***LeuCysAspGlnGly***LysLeuHisProLe 602486 : ....ag{g}catgtggggtcttagctctgTGACCAGGGATAGAAGCTGCACcctct : 680289 144 : uTrpLysTrpMetLysIleGlnProLysGlyLysGlyIleLeuGlyAsnAla{I} : 161 |::: ||| ||| !! !...||| !||| !!:! { } uTyrTrpLysAlaLysSer***ProAlaAspSerGlyGluLeuProSerPro{T}+ 680290 : gtattggaaggcaaagtcctaaccagcGGATTCCGGGGAACTCCCCAGCCca{t}g : 680344 162 : >>>> Target Intron 16 >>>> {le}LysTrpAsnPheThrLysPheLe : 169 183429 bp { !}||| .!.||| ! ! ! + ++{yr}LysIleGlnPheGlnValGlyGl 680345 : t..........................ag{AT}AAAATTCAATTCCAGGTGGGGCA : 863796 170 : uIleAspLysAsnGlyCysValValLysArgTyr{G} >>>> Target Intro : 181 ! ! ! .....!|||:!! !..!!:!:!!{!} 95244 bp nCysProGlyArgSerCysLeuArgSerGlnHis{A}++ 863797 : GTGTCCCGGCCGGTCATGCCTGAGGTCGCAGCAT{G}gt................. : 863835 182 : n 17 >>>> {ly}ProMetGluGluProLeuValIleGlu >>>> Target : 191 { !}|||..!! ...|||! ! !:!!||| 522 ++{sp}ProGlnAlaSerProSerHisLeuGlu++ 863836 : .........ag{AT}CCGCAGGCCTCCCCCTCACATCTCGAGgt............ : 959108 192 : Intron 18 >>>> >>>> Target Intron 19 >>>> >>>> Tar : 191 71 bp 100771 bp ++++ ++++ 959109 : ..............aggt..........................aggt........ : 1112150 192 : get Intron 20 >>>> LysAspLeuProHis : 195 60054 bp :::||||||||| ++GlyAsnLeuProHis 1112151 : ..................aggggaatcttccccac : 1172216 vulgar: SPP00000007_1.0 3 195 . NIVO01030317.1:subseq(555646,1182752) 58041 1172216 + 142 M 6 18 S 0 2 5 0 2 I 0 21344 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 38888 3 0 2 S 1 1 M 3 9 5 0 2 I 0 52721 3 0 2 M 1 3 S 0 1 5 0 2 I 0 1401 3 0 2 S 1 2 M 6 18 S 0 2 5 0 2 I 0 20813 3 0 2 S 1 1 M 9 27 S 0 1 5 0 2 I 0 323 3 0 2 S 1 2 M 1 3 5 0 2 I 0 65083 3 0 2 M 12 36 S 0 2 5 0 2 I 0 93244 3 0 2 S 1 1 M 17 51 5 0 2 I 0 63025 3 0 2 M 1 3 S 0 1 5 0 2 I 0 37688 3 0 2 S 1 2 M 19 57 S 0 1 5 0 2 I 0 30730 3 0 2 S 1 2 M 17 51 5 0 2 I 0 100903 3 0 2 M 10 30 5 0 2 I 0 16852 3 0 2 M 6 18 5 0 2 I 0 999 3 0 2 S 0 2 5 0 2 I 0 77756 3 0 2 S 1 1 M 33 99 S 0 1 5 0 2 I 0 183425 3 0 2 S 1 2 M 19 57 S 0 1 5 0 2 I 0 95240 3 0 2 S 1 2 M 9 27 5 0 2 I 0 52267 3 0 2 5 0 2 I 0 100767 3 0 2 5 0 2 I 0 60050 3 0 2 M 5 15 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 58042 1172216 142 + . gene_id 6 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 58042 58061 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 58042 58061 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 58062 58063 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 58062 79409 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 79408 79409 . + . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 79410 79433 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 79410 79433 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 79434 79435 . + . intron_id 2 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 79434 118325 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 118324 118325 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 118326 118335 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 118326 118335 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 118336 118337 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 118336 171060 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 172469 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 172468 172469 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 172470 172491 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 172470 172491 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 172492 172493 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 172492 193308 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 193307 193308 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 193309 193337 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 193309 193337 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 193338 193339 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 193338 193664 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 193663 193664 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 193665 193669 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 193665 193669 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 193670 193671 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 193670 258756 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 258755 258756 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 258757 258794 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 258757 258794 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 258795 258796 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 258795 352042 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 352041 352042 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 352043 352094 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 352043 352094 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 352095 352096 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 352095 415123 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 415122 415123 . + . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 415124 415127 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 415124 415127 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 415128 415129 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 415128 452819 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 452818 452819 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 452820 452879 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 452820 452879 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 452880 452881 . + . intron_id 11 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 452880 483613 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 483612 483613 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 483614 483666 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 483614 483666 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 483667 483668 . + . intron_id 12 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 483667 584573 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 584572 584573 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 584574 584603 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 584574 584603 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 584604 584605 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 584604 601459 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 602480 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 602479 602480 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 602481 602482 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 602481 602482 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 602483 602484 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 602483 680242 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 680241 680242 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 680243 680343 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 680243 680343 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 680344 680345 . + . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 680344 863772 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863832 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863832 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863833 863834 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863833 959076 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 959075 959076 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 959077 959105 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 959077 959105 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 959106 959107 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 959106 1011376 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1112147 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1172201 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1172200 1172201 . + . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1172202 1172216 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1172202 1172216 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 58042 1172216 142 + . alignment_id 6 ; Query SPP00000007_1.0 ; Align 58042 4 18 ; Align 79411 11 21 ; Align 118327 19 9 ; Align 171061 22 3 ; Align 172472 24 18 ; Align 193310 31 27 ; Align 193667 41 3 ; Align 258757 42 36 ; Align 352044 55 51 ; Align 415124 72 3 ; Align 452822 74 57 ; Align 483616 94 51 ; Align 584574 111 30 ; Align 601460 121 18 ; Align 680244 128 99 ; Align 863775 162 57 ; Align 959079 182 27 ; Align 1172202 191 15 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 141 Query range: 0 -> 197 Target range: 137522 -> 1148269 1 : MetSerLeuGlyArgLeuCysArgLeuLeuLys >>>> Target Intron 1 : 12 :!:!!!|||||||||:!! !!! !:!!:!!:!! 33505 bp LeuThrLeuGlyArgValGlyThrValValGln++ 137523 : CTCACCTTGGGCAGGGTGGGCACCGTTGTCCAGgt..................... : 137558 13 : >>>> Pro{A} >>>> Target Intron 2 >>>> {la}LeuLeuCysGl : 17 |||{!} 33295 bp {.!}::: .. ++Pro{G}++ ++{ly}IleGluProAl 137559 : ....agCCA{G}gt.........................ag{gg}atcgaacctgc : 204371 18 : yAlaLeuAlaAlaProGlyLeuAla{G} >>>> Target Intron 3 >>>> : 26 .:::|||...||||||...||||||{|} 18643 bp aSerLeuValAlaProAlaLeuAla{G}++ - 204372 : atctcttgtggctcctgcactggca{g}gt.........................t : 223040 27 : {ly}ThrMetCys >>>> Target Intron 4 >>>> AlaSerArgAspA : 34 {||}:!!:!!||| 161109 bp ...:::||| | +{ly}SerValCys++ ++GlyThrArgGlyA 223041 : g{ga}TCTGTGTGCgt.........................aggggacacgaggGG : 384174 35 : spTrpArgCys{A} >>>> Target Intron 5 >>>> {la}ArgSerMet : 41 |||||||| {!} 90237 bp {.!} |||::: spTrpArgPro{G}++ ++{ly}SerSerVal 384175 : ACTGGAGGCCa{g}gt.........................ag{gc}tcctctgtc : 474432 42 : HisGluPheSerAla{L} >>>> Target Intron 6 >>>> {ys}AspIl : 49 ||| ||| { } 84277 bp { !}||| HisGlyAspSerPro{G}++ ++{ly}AspAl 474433 : cacggggattctcca{g}gt.........................ag{GA}GACGC : 558733 50 : eAspGlyHis{M} >>>> Target Intron 7 >>>> {et}ValAsnLeuA : 57 !! ||||||{ } 71517 bp { } ||||||. aAlaGlyHis{A}++ ++{la}PheAsnLeuS 558734 : CGCAGGACAT{G}gt.........................ag{cc}ttcaatcttt : 630274 58 : spLysTyrArgGlyPheVal{Cy} >>>> Target Intron 8 >>>> {s} : 64 ..:::::::::|||... { } 5760 bp { } erGlnHisGlnGlyLeuPhe{Gl}++ -+{n} 630275 : cccagcatcagggtcttttc{ca}gt.........................tg{g} : 636057 65 : IleValThr >>>> Target Intron 9 >>>> >>>> Target Intr : 68 |||:!!||| 30591 bp 8808 b IleLeuThr++ ++++ 636058 : atTTTGACAgt.........................aggt................ : 666660 69 : on 10 >>>> AsnValAlaSerGlnUnkGlyLysThrGluValAsnTyrThrGl : 82 p :!! !!.!:!!::! ||| ! !.!.! !|||!! !|| ++AspSerValAlaArgProGlyValPheHisGlyAsn***LeuGl 666661 : ..........agGACTCAGTAGCCAGACCGGGTGTGTTCCATGGAAATTAATTGCA : 675508 83 : nLeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArgIle >>>> Tar : 98 ||||:!! ! ||| !! !!:!!:! !...||| !||| ! nLeuIleLysLeuAspHisGlnPheLeuSerCysProLeuSerGln++ 675509 : ATTAATTAAATTAGACCATCAGTTCCTCAGTTGCCCCCTGTCACAGgt........ : 675558 99 : get Intron 11 >>>> LeuAlaPheProCys{As} >>>> Target Int : 103 75523 bp |||||| {..} 11340 ++GlyMetPheProThr{Ar}+- 675559 : ..................aggggatgttcccgacc{cg}gg............... : 751098 104 : ron 12 >>>> {n}GlnPheGlyLysGlnGluProGly{S} >>>> Target : 112 bp {.} |||...:::|||||| {!} 31 ++{g}GlyPheSerArgGlnGluAspTrp{L}++ 751099 : ...........ag{g}ggattctccaggcaggaagactgg{a}gt........... : 762464 113 : Intron 13 >>>> {er}AsnGluGluIleLysGluPheAlaAlaGlyTyrAs : 124 357 bp {.!}.!.|||... !! ..! ! !||| !. ++{ys}GlnGluSerAspThrArgArgMetArgGlyGlyAr 762465 : ...............ag{AG}CAGGAAAGCGACACTAGAAGAATGAGGGGTGGAAG : 793854 125 : nValLysPheAspMetPheSerLysIleCysVal{A} >>>> Target Intro : 136 . !|||.!.! !:!:.!!! !::!:!!||||||{.} 66010 bp gTyrLysMetGlyLeuIleLeuArgValCysVal{G}++ 793855 : ATATAAAATGGGTCTAATTTTACGAGTCTGTGTT{G}gt................. : 793893 137 : n 14 >>>> {sn}GlyAspAspAlaHisProLeuTrpLysTrpMetLysIleGl : 150 {..}||| !! !||||||||||||! ! |||:!!|| -+{ly}GlyHisProArgHisProLeuTrpThrThrSerLysLeuGl 793894 : .........cg{GG}GGGCATCCGCGTCACCCACTTTGGACCACTAGCAAACTCCA : 859942 151 : n >>>> Target Intron 15 >>>> ProLysGlyLysGlyIle >>>> : 157 | 32152 bp !!||||||!:!|||! n+- ++AlaLysGlyArgGlyThr++ 859943 : Aga..........................agGCGAAGGGTAGGGGGACGgt..... : 892117 158 : Target Intron 16 >>>> LeuGlyAsnAlaIleLysTrpAsnPheThrLys : 167 69655 bp ! !!:! !:!:! ||| ::: ++AlaThrSerTrpValMetTrpPheSerGlnGlu 892118 : .....................agGCAACAAGCTGGGTGATgtggttttcccaggag : 961800 168 : PheLeuIleAsp >>>> Target Intron 17 >>>> >>>> Target : 172 :::||| 49562 bp 100 SerCysMetAsp++ ++++ 961801 : tcatgtatggatgt..........................aggt............ : 1011379 173 : Intron 18 >>>> >>>> Target Intron 19 >>>> LysAsnGlyC : 175 771 bp 25340 bp !:::|||| ++++ ++ValSerGlyC 1011380 : ..............aggt..........................agGTGTCAGGGT : 1137497 176 : ysValValLysArgTyr{G} >>>> Target Intron 20 >>>> {ly}Pr : 182 || !||| ! ! ! {|} 553 bp {||}|| ysTrpValHisThrVal{G}+- ++{ly}Pr 1137498 : GTTGGGTGCACACGGTG{G}gc..........................ag{GG}CC : 1138071 183 : oMetGluGluPro{L} >>>> Target Intron 21 >>>> {eu}ValIle : 189 |! !|||.!.|||{ } 10151 bp { !}||| ! oArgGluHisPro{G}++ ++{lu}ValSer 1138072 : GAGGGAGCACCCA{G}gt..........................ag{AG}GTCTCC : 1148243 190 : GluLysAspLeuProHisTyrPhe : 197 :!!|||!!::!!|||... ||| GlnLysGluValProArgLeuPhe 1148244 : CAGAAGGAGGTGCCAAGGCTGTTT : 1148269 vulgar: SPP00000007_1.0 0 197 . NIVO01030317.1:subseq(555646,1182752) 137522 1148269 + 141 M 11 33 5 0 2 I 0 33501 3 0 2 M 1 3 S 0 1 5 0 2 I 0 33291 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 18639 3 0 2 S 1 2 M 3 9 5 0 2 I 0 161105 3 0 2 M 8 24 S 0 1 5 0 2 I 0 90233 3 0 2 S 1 2 M 8 24 S 0 1 5 0 2 I 0 84273 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 71513 3 0 2 S 1 2 M 10 30 S 0 2 5 0 2 I 0 5756 3 0 2 S 1 1 M 3 9 5 0 2 I 0 30587 3 0 2 5 0 2 I 0 8804 3 0 2 M 30 90 5 0 2 I 0 75519 3 0 2 M 5 15 S 0 2 5 0 2 I 0 11336 3 0 2 S 1 1 M 8 24 S 0 1 5 0 2 I 0 31353 3 0 2 S 1 2 M 23 69 S 0 1 5 0 2 I 0 66006 3 0 2 S 1 2 M 14 42 5 0 2 I 0 32148 3 0 2 M 6 18 5 0 2 I 0 69651 3 0 2 M 15 45 5 0 2 I 0 49558 3 0 2 5 0 2 I 0 100767 3 0 2 5 0 2 I 0 25336 3 0 2 M 9 27 S 0 1 5 0 2 I 0 549 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 10147 3 0 2 S 1 2 M 10 30 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 137523 1148269 141 + . gene_id 7 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 137523 137555 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 137523 137555 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 137556 137557 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 137556 171060 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 204359 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 204358 204359 . + . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 204360 204398 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 204360 204398 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 204399 204400 . + . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 204399 223041 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 223040 223041 . + . intron_id 2 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 223042 223052 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 223042 223052 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 223053 223054 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 223053 384161 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 384160 384161 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 384162 384186 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 384162 384186 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 384187 384188 . + . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 384187 474423 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 474422 474423 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 474424 474450 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 474424 474450 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 474451 474452 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 474451 558727 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 558726 558727 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 558728 558745 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 558728 558745 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 558746 558747 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 558746 630262 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 630261 630262 . + . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 630263 630296 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 630263 630296 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 630297 630298 . + . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 630297 636056 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 636055 636056 . + . intron_id 7 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 636057 636066 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 636057 636066 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 636067 636068 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 636067 666657 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 675465 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 675464 675465 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 675466 675555 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 675466 675555 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 675556 675557 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 675556 751078 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 751077 751078 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 751079 751095 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 751079 751095 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 751096 751097 . + . intron_id 12 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 751096 762435 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 762434 762435 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 762436 762461 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 762436 762461 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 762462 762463 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 762462 793818 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 793817 793818 . + . intron_id 12 ; splice_site "aG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 793819 793890 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 793819 793890 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 793891 793892 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 793891 859900 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 859899 859900 . + . intron_id 13 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 859901 859944 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 859901 859944 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 859945 859946 . + . intron_id 15 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 859945 892096 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 892095 892096 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 892097 892114 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 892097 892114 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 892115 892116 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 892115 961769 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 961768 961769 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 961770 961814 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 961770 961814 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 961815 961816 . + . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 961815 1011376 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1112147 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1137487 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1137486 1137487 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1137488 1137515 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1137488 1137515 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1137516 1137517 . + . intron_id 20 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1137516 1138068 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1138067 1138068 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1138069 1138086 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1138069 1138086 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1138087 1138088 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1138087 1148237 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1148236 1148237 . + . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1148238 1148269 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1148238 1148269 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 137523 1148269 141 + . alignment_id 7 ; Query SPP00000007_1.0 ; Align 137523 1 33 ; Align 171061 12 3 ; Align 204362 14 36 ; Align 223044 27 9 ; Align 384162 30 24 ; Align 474426 39 24 ; Align 558730 48 15 ; Align 630265 54 30 ; Align 636058 65 9 ; Align 675466 68 90 ; Align 751079 98 15 ; Align 762437 104 24 ; Align 793821 113 69 ; Align 859903 137 42 ; Align 892097 151 18 ; Align 961770 157 45 ; Align 1137488 172 27 ; Align 1138071 182 15 ; Align 1148240 188 30 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 140 Query range: 1 -> 195 Target range: 28017 -> 1149602 2 : SerLeuGlyArgLeuCysArgLeuLeuLysProAla >>>> Target Intron : 14 .!!|||||| !! !||||||||||||:!!! ! ! 31601 bp GlyLeuGlyAspHisCysArgLeuLeuGlnGlnHis++ 28018 : GGCCTTGGAGACCATTGCCGTCTCCTTCAGCAGCACgt.................. : 28056 15 : 1 >>>> LeuLeuCysGlyAlaLeuAlaAlaPro >>>> Target Intron : 23 |||||| ||| !||| ! !||| 12576 bp ++LeuLeuLeuGlyGlnLeuHisGlnPro++ 28057 : .......agCTCCTCCTGGGCCAGCTCCACCAGCCGgt.................. : 59684 24 : 2 >>>> GlyLeuAlaGlyThrMetCysAlaSerArgAspAsp{T} >>>> T : 35 ||||||||| ||| !.!||||||!!:|||{|} ++GlyLeuAla---GluMetGlnGlySerArgGluAsp{T}++ 59685 : .......agggcctggca---gagATGCAAGGATCCAGGGAAGAT{T}gt...... : 72294 36 : arget Intron 3 >>>> {rp}ArgCysAlaArgSerMetHis{Gl} >>>> : 43 21930 bp {||}||| ! ::!||| !||| !{..} ++{rp}ArgArgSerArgValMetGly{Ar}++ 72295 : ...................ag{GG}AGGAGGAGCCGGGTGATGGGC{AG}gt.... : 94249 44 : Target Intron 4 >>>> {u}PheSerAlaLysAspIleAspGlyHisMet : 53 1391 bp {!} !|||::!:!! ! ! !.! :!! ++{g}AlaSerSerGluProAlaLysAlaGlyVal 94250 : .....................ag{G}GCCAGTAGTGAGCCGGCCAAGGCGGGGGTG : 95666 54 : Val{A} >>>> Target Intron 5 >>>> {sn}LeuAspLysTyrArgGl : 61 |||{.} 50708 bp {..} !||| !! ! Val{G}+- ++{ly}ArgAspAlaLeuIlePr 95667 : GTT{G}gc.........................ag{GA}AGGGATGCCCTCATACC : 146398 62 : yPheValCysIleValThrAsnValAlaSerGln >>>> Target Intron 6 : 73 ! ||||||:!: ! !!.....!::!|||||| 5534 bp oGlyValCysLeuGlnProGlyThrSerSerGln++ 146399 : TGGGGTGTGCTTGCAACCTGGGACCAGCAGCCAGgt.................... : 146436 74 : >>>> UnkGlyLysThrGluValAsnTyrThrGlnLeuValAspLeuHisAla : 89 ||| ! |||! :!!!:!||| !!! !!.! :!! !.! ++GluGlyAspThrGlyLeuSerTyrProProAlaAlaArgValGlyVal+ 146437 : .....agGAGGGTGACACAGGCCTCAGCTATCCGCCTGCTGCCAGGGTGGGGGTGg : 152016 90 : >>>> Target Intron 7 >>>> ArgTyrAlaGluCysGlyLeuArgIleL : 98 57027 bp ! ! |||:!!...||||||||| !| - ++LeuProAlaGlnAlaGlyLeuArgAlaL 152017 : c.........................agCTCCCAGCACAGGCAGGGTTGAGGGCCC : 209070 99 : eu >>>> Target Intron 8 >>>> AlaPheProCysAsnGlnPheGlyL : 107 || 64347 bp ||||||! ! ! |||!!..!!||| eu++ ++AlaPheGlnGlyAsnHisLeuGlyA 209071 : TGgt.........................agGCCTTTCAAGGGAACCACCTTGGGG : 273444 108 : ysGlnGluProGlySer{As} >>>> Target Intron 9 >>>> {n}Glu : 114 !:!!||| ! !:!!{!.} 125138 bp {.} laGluGluLysLysAla{Ar}++ ++{g}Pro 273445 : CAGAGGAGAAAAAGGCT{AG}gt.........................ag{A}CCT : 398603 115 : GluIleLys >>>> Target Intron 10 >>>> GluPheAlaAlaGlyTy : 123 |||:!!||| 36298 bp ! !||| !!: GluValLys++ ++AlaAlaArgAlaProTr 398604 : GAAGTCAAGgt..........................agGCTGCACGGGCGCCCTG : 434928 124 : rAsnValLysPheAspMetPheSerLysIleCys{Va} >>>> Target Intr : 135 : !..!!..!!...!! ! !!!!::::!!|||{ } 14690 b pProThrSerLeuSerArgSerThrArgValCys{Ar}++ 434929 : GCCCACAAGCTTGTCCAGGAGTACCCGCGTCTGC{AG}gt................ : 434968 136 : on 11 >>>> {l}AsnGlyAspAspAlaHisProLeuTrp{L} >>>> Targ : 145 p {!}..!|||:!! !||| ! !!:!!|||{ } ++{g}ArgGlyAsnProAlaSerAlaValTrp{A}++ 434969 : ..........ag{G}CGTGGCAACCCCGCCTCTGCGGTTTGG{G}gt......... : 449687 146 : et Intron 12 >>>> {ys}TrpMetLysIleGlnProLysGlyLys{G} > : 155 4371 bp { }|||! ! ||| |||..!{|} ++{la}TrpSerProArgPheProAlaGlySer{G}++ 449688 : .................ag{CC}TGGAGCCCCAGGTTTCCTGCTGGGTCA{G}gt. : 454088 156 : >>> Target Intron 13 >>>> {ly}Ile{Le} >>>> Target Intr : 157 8544 bp {||}:::{:!} 139844 ++{ly}Val{Me}++ 454089 : .........................ag{ga}gtc{at}gt................ : 462639 158 : on 14 >>>> {u}{G} >>>> Target Intron 15 >>>> {ly}AsnA : 160 bp {!}{|} 126630 bp {||}:::: ++{t}{G}++ ++{ly}SerS 462640 : ..........ag{g}{g}gt..........................ag{gc}tcct : 729118 161 : laIleLysTrpAsnPheThr >>>> Target Intron 16 >>>> LysPhe : 168 ::::: ||| ||| 48854 bp :!!||| erValHisTrpIlePheGln+- ++GluPhe 729119 : ccgtccattggattttccaggc..........................agGAGTTT : 777996 169 : LeuIleAspLysAsnGlyCysValValLysArgTyrGlyPro >>>> Target : 183 :!!:!:! !..!||| !||| !!.! !! !||| 101 ValValAlaSerAsnThrCysTrpAlaCysGlyLeuThrPro++ 777997 : GTGGTGGCTTCAAACACGTGCTGGGCGTGTGGACTGACACCTgt............ : 778043 184 : Intron 17 >>>> MetGluGluPro{L} >>>> Target Intron 18 > : 187 63 bp ||||||||||||{ } 75556 bp ++MetGluGluPro{A}++ 778044 : ..............agatggaggagcct{g}gt....................... : 788219 188 : >>> {eu}ValIleGluLysAsp >>>> Target Intron 19 >>>> : 193 { !} !|||:!! .!. 147587 bp ++{sp}LysIleGlnPheGln++ ++++ 788220 : ...ag{AT}AAAATTCAATTCCAGgt..........................aggt : 1011377 194 : >>>> Target Intron 20 >>>> >>>> Target Intron 21 >>>> : 193 100771 bp 37446 bp ++++ 1011378 : ..........................aggt.......................... : 1112150 194 : LeuProHis : 195 ||||||||| ++LeuProHis 1112151 : agCTGCCCCAC : 1149602 vulgar: SPP00000007_1.0 1 195 . NIVO01030317.1:subseq(555646,1182752) 28017 1149602 + 140 M 12 36 5 0 2 I 0 31597 3 0 2 M 9 27 5 0 2 I 0 12572 3 0 2 M 3 9 G 1 0 M 8 24 S 0 1 5 0 2 I 0 21926 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 1387 3 0 2 S 1 1 M 11 33 S 0 1 5 0 2 I 0 50704 3 0 2 S 1 2 M 17 51 5 0 2 I 0 5530 3 0 2 M 16 48 5 0 2 I 0 57023 3 0 2 M 10 30 5 0 2 I 0 64343 3 0 2 M 14 42 S 0 2 5 0 2 I 0 125134 3 0 2 S 1 1 M 4 12 5 0 2 I 0 36294 3 0 2 M 17 51 S 0 2 5 0 2 I 0 14686 3 0 2 S 1 1 M 9 27 S 0 1 5 0 2 I 0 4367 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 8540 3 0 2 S 1 2 M 1 3 S 0 2 5 0 2 I 0 139840 3 0 2 S 1 2 5 0 2 I 0 126626 3 0 2 S 1 2 M 8 24 5 0 2 I 0 48850 3 0 2 M 16 48 5 0 2 I 0 10159 3 0 2 M 4 12 S 0 1 5 0 2 I 0 75552 3 0 2 S 1 2 M 5 15 5 0 2 I 0 147583 3 0 2 5 0 2 I 0 100767 3 0 2 5 0 2 I 0 37442 3 0 2 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 28018 1149602 140 + . gene_id 8 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 28018 28053 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 28018 28053 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 28054 28055 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 28054 59654 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 59653 59654 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 59655 59681 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 59655 59681 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 59682 59683 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 59682 72257 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 72256 72257 . + . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 72258 72291 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 72258 72291 . + . insertions 0 ; deletions 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 72292 72293 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 72292 94221 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 94220 94221 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 94222 94246 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 94222 94246 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 94247 94248 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 94247 95637 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 95636 95637 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 95638 95672 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 95638 95672 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 95673 95674 . + . intron_id 5 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 95673 146380 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146379 146380 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146381 146433 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146381 146433 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146434 146435 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146434 151967 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 151966 151967 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 151968 152015 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 151968 152015 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 152016 152017 . + . intron_id 7 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 152016 209042 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 209041 209042 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 209043 209072 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 209043 209072 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 209073 209074 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 209073 273419 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 273418 273419 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 273420 273463 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 273420 273463 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 273464 273465 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 273464 398601 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 398600 398601 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 398602 398614 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 398602 398614 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 398615 398616 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 398615 434912 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 434911 434912 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 434913 434965 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 434913 434965 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 434966 434967 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 434966 449655 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 449654 449655 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 449656 449684 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 449656 449684 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 449685 449686 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 449685 454055 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 454054 454055 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 454056 454085 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 454056 454085 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 454086 454087 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 454086 462629 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462636 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462636 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462637 462638 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462637 602480 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 602479 602480 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 602481 602482 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 602481 602482 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 602483 602484 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 602483 729112 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 729111 729112 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 729113 729138 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 729113 729138 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 729139 729140 . + . intron_id 16 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 729139 777992 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 777991 777992 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 777993 778040 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 777993 778040 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 778041 778042 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 778041 788203 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 788202 788203 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 788204 788216 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 788204 788216 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 788217 788218 . + . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 788217 863772 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 1011376 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1112147 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1149593 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1149592 1149593 . + . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1149594 1149602 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1149594 1149602 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 28018 1149602 140 + . alignment_id 8 ; Query SPP00000007_1.0 ; Align 28018 2 36 ; Align 59655 14 27 ; Align 72258 23 9 ; Align 72267 27 24 ; Align 94224 36 21 ; Align 95639 44 33 ; Align 146383 56 51 ; Align 151968 73 48 ; Align 209043 89 30 ; Align 273420 99 42 ; Align 398603 114 12 ; Align 434913 118 51 ; Align 449657 136 27 ; Align 454058 146 27 ; Align 462632 156 3 ; Align 729115 159 24 ; Align 777993 167 48 ; Align 788204 183 12 ; Align 863775 188 15 ; Align 1149594 193 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 131 Query range: 13 -> 196 Target range: 210 -> 778544 14 : LeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAla{Gl} >>>> Target Intr : 26 ||| !||| !!..!||| ! !||| !!|||!..{ !} 11965 b LeuArgCysArgCysLeuPheLeuProTrpLeuGly{Ar}++ 211 : CTCAGGTGCAGGTGTCTCTTCTTGCCCTGGCTGGGa{ag}gt................ : 251 27 : on 1 >>>> {y}ThrMetCysAlaSerArgAspAspTrpArgCysAlaArgSerMe : 41 p {!}..!! ! |||!!!||| ! |||! ! !.!|||!.! ++{g}ValLysLeuAla***ArgThrAlaTrpMetLeuGlyArgLysGl 252 : .........ag{G}GTGAAATTGGCTTGAAGAACAGCATGGATGCTGGGCAGAAAAGG : 12257 42 : tHisGluPheSerAlaLysAspIleAsp >>>> Target Intron 2 >>>> G : 51 ! !:!! :!! ! !! !:!: 38431 bp ySerGlnAlaAlaArgProAlaLeuPro++ ++A 12258 : GAGCCAGGCAGCCAGGCCAGCTCTGCCGgt.........................agC : 50718 52 : lyHisMetValAsnLeuAspLys{T} >>>> Target Intron 3 >>>> {yr : 59 !!|||...:!!.!.||| !!:!!{ } 7387 bp {! rgHisGlnMetGluLeuHisGln{G}++ ++{lu 50719 : GCCATCAAATGGAACTTCACCAA{G}gt.........................ag{AA : 58130 60 : }ArgGlyPheValCysIleVal{Th} >>>> Target Intron 4 >>>> {r} : 67 }|||||| |||||| ! !{! } 1713 bp {!} }ArgGlyAlaValCysAlaCys{Ar}++ ++{g} 58131 : }CGGGGTGCAGTGTGTGCCTGC{AG}gt.........................ag{A} : 59868 68 : AsnValAlaSerGlnUnkGlyLysThrGluValAsnTyrThrGlnLeuVal >>>> : 85 ||||||!.! !!:!! ||| !:!! !..!||||||:!! !:!! AsnValGlyProGluAsnGlyGlyCysLysTrpGlyTyrThrGluAlaLeu+- 59869 : AATGTAGGGCCAGAGAATGGGGGCTGTAAGTGGGGCTATACAGAGGCCCTGgc..... : 59922 86 : Target Intron 5 >>>> AspLeuHisAlaArgTyrAlaGluCysGlyLeuArg : 96 31481 bp ||| !|||! ! !! ! ||| !||| ++ProLeuAspAlaLeuLeuProTrpGluGlyAlaArg 59923 : ....................agccccttgaTGCCCTTCTGCCTTGGGAAGGGGCTCGA : 91434 97 : IleLeu{Al} >>>> Target Intron 6 >>>> {a}PheProCysAsnGlnP : 105 ||||||{! } 33066 bp {!}.!.||||||!..!::. IleLeu{Gl}++ ++{u}LeuProCysArgArgL 91435 : ATTCTC{ga}gt.........................ag{A}CTGCCTTGCAGGCGTC : 124527 106 : heGlyLysGlnGluProGlySerAsnGluGlu >>>> Target Intron 7 >>> : 116 !...! !!..!.|||.!!|||!:!|||||| 2469 bp euSerLeuHisAsnProSerSerSerGluGlu+- 124528 : TGTCCCTCCATAATCCCAGCAGCAGCGAGGAGgc........................ : 124562 117 : > IleLysGluPhe{Al} >>>> Target Intron 8 >>>> {a}AlaGlyT : 123 |||::!:!! !{ } 1990 bp {!}||| !! ++IleArgGlnHis{Ar}++ ++{g}AlaGlnT 124563 : .agATCCGGCAGCAC{AG}gt.........................ag{G}GCCCAGT : 129040 124 : yrAsnValLysPheAspMet >>>> Target Intron 9 >>>> PheSerLys : 132 :: !:!! ||| !..! 106796 bp |||.!!||| rpAlaLeuAlaPheThrGln++ ++PheGlyLys 129041 : GGGCTCTGGCCTTCACCCAGgt.........................agTTTGGGAAG : 235863 133 : IleCysValAsnGlyAspAsp{A} >>>> Target Intron 10 >>>> {la} : 140 ! :::|||||||||{|} 35357 bp {||} ThrGluSerSerGlyAspAsp{A}++ ++{la} 235864 : Acggaaagttctggagatgat{g}gt..........................ag{CC} : 271245 141 : HisProLeuTrpLysTrp >>>> Target Intron 11 >>>> MetLysIleG : 150 |||||| !|||:::||| 101094 bp :!!:!!|||! HisProThrTrpArgTrp++ ++LeuGluIleH 271246 : CATCCCACCTGGCGTTGGgt..........................agTTGGAGATAC : 372368 151 : lnProLys{Gl} >>>> Target Intron 12 >>>> {y}LysGlyIleLeuG : 158 !.|||:!!{ } 77277 bp {!}:::|||! !! !! isProGln{Le}++ ++{u}ArgGlyAsnProA 372369 : ACCCCCAG{CT}gt..........................ag{G}CGTGGCAACCCCG : 449669 159 : lyAsnAlaIleLys{T} >>>> Target Intron 13 >>>> {rp}AsnPheT : 166 .!::!|||:!! !{ } 11619 bp {! }:::.!! laSerAlaValTrp{G}++ -+{ly}SerIleL 449670 : CCTCTGCGGTTTGG{G}gt..........................tg{ga}tctATCC : 461312 167 : hrLysPheLeuIle{A} >>>> Target Intron 14 >>>> {sp}LysAsnG : 174 !||||||||||||{!} 142970 bp { }!:!!!.| euLysPheLeuIle{G}++ ++{ly}ArgLysG 461313 : TTAAGTTCTTAATC{G}gt..........................ag{GA}AGAAAGG : 604306 175 : lyCysValValLys >>>> Target Intron 15 >>>> >>>> Target : 179 ||||||||! !:!! 62337 bp 527 lyCysValAspGln++ ++++ 604307 : GCTGTGTGGACCAGgt..........................aggt............ : 666660 180 : Intron 16 >>>> ArgTyrGlyProMet >>>> Target Intron 17 >>> : 184 79 bp ! !! !||||||..! 59054 bp ++MetCysGlyProGln+- 666661 : ..............agATGTGTGGTCCCCAGgg......................... : 719454 185 : > GluGluProLeuValIleGluLysAspLeuProHisTyr : 196 ..!! |||||| !:!:.!.!:!||||||||| !!:! ++ArgAlaProLeuCysValHisArgAspLeuProAlaPhe 719455 : .agCGAGCCCCCCTGTGTGTGCACAGGGATCTGCCTGCTTTC : 778544 vulgar: SPP00000007_1.0 13 196 . NIVO01030317.1:subseq(555646,1182752) 210 778544 + 131 M 12 36 S 0 2 5 0 2 I 0 11961 3 0 2 S 1 1 M 24 72 5 0 2 I 0 38427 3 0 2 M 8 24 S 0 1 5 0 2 I 0 7383 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 1709 3 0 2 S 1 1 M 17 51 5 0 2 I 0 31477 3 0 2 M 14 42 S 0 2 5 0 2 I 0 33062 3 0 2 S 1 1 M 16 48 5 0 2 I 0 2465 3 0 2 M 4 12 S 0 2 5 0 2 I 0 1986 3 0 2 S 1 1 M 9 27 5 0 2 I 0 106792 3 0 2 M 10 30 S 0 1 5 0 2 I 0 35353 3 0 2 S 1 2 M 6 18 5 0 2 I 0 101090 3 0 2 M 6 18 S 0 2 5 0 2 I 0 77273 3 0 2 S 1 1 M 9 27 S 0 1 5 0 2 I 0 11615 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 142966 3 0 2 S 1 2 M 7 21 5 0 2 I 0 62333 3 0 2 5 0 2 I 0 52775 3 0 2 M 5 15 5 0 2 I 0 59050 3 0 2 M 13 39 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 211 778544 131 + . gene_id 9 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 211 248 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 211 248 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 249 250 . + . intron_id 1 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 249 12213 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 12212 12213 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 12214 12286 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 12214 12286 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 12287 12288 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 12287 50717 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 50716 50717 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 50718 50742 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 50718 50742 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 50743 50744 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 50743 58129 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 58128 58129 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 58130 58154 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 58130 58154 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 58155 58156 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 58155 59867 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 59866 59867 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 59868 59919 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 59868 59919 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 59920 59921 . + . intron_id 5 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 59920 91400 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 91399 91400 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 91401 91444 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 91401 91444 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 91445 91446 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 91445 124510 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 124509 124510 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 124511 124559 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 124511 124559 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 124560 124561 . + . intron_id 7 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 124560 127028 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 127027 127028 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 127029 127042 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 127029 127042 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 127043 127044 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 127043 129032 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 129031 129032 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 129033 129060 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 129033 129060 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 129061 129062 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 129061 235856 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 235855 235856 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 235857 235887 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 235857 235887 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 235888 235889 . + . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 235888 271244 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 271243 271244 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 271245 271264 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 271245 271264 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 271265 271266 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 271265 372358 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 372357 372358 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 372359 372378 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 372359 372378 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 372379 372380 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 372379 449655 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 449654 449655 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 449656 449684 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 449656 449684 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 449685 449686 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 449685 461303 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 461302 461303 . + . intron_id 12 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 461304 461327 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 461304 461327 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 461328 461329 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 461328 604297 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 604296 604297 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 604298 604320 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 604298 604320 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 604321 604322 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 604321 666657 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 719436 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 719435 719436 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 719437 719451 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 719437 719451 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 719452 719453 . + . intron_id 17 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 719452 778505 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 778504 778505 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 778506 778544 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 778506 778544 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 211 778544 131 + . alignment_id 9 ; Query SPP00000007_1.0 ; Align 211 14 36 ; Align 12215 27 72 ; Align 50718 51 24 ; Align 58132 60 21 ; Align 59869 68 51 ; Align 91401 85 42 ; Align 124512 100 48 ; Align 127029 116 12 ; Align 129034 121 27 ; Align 235857 130 30 ; Align 271247 141 18 ; Align 372359 147 18 ; Align 449657 154 27 ; Align 461306 164 21 ; Align 604300 172 21 ; Align 719437 179 15 ; Align 778506 184 39 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 131 Query range: 1 -> 196 Target range: 17813 -> 1112171 2 : SerLeuGlyArgLeuCysArgLeuLeuLysPro{A} >>>> Target Intron : 13 :!!:!!! !|||||||||! ! !:!! |||{|} 45868 bp AlaValAspArgLeuCysLeuGlyIleProPro{A}++ 17814 : GCGGTTGACCGCCTGTGCCTGGGGATCCCCCCT{G}gt.................. : 17850 14 : 1 >>>> {la}LeuLeuCysGlyAlaLeuAlaAla{Pr} >>>> Target I : 22 {||}||| !|||||| !!!!..!|||{ } 4478 +-{la}LeuAlaCysGlyTrpPheCysAla{Ar}++ 17851 : .......aa{CA}TTGGCCTGTGGCTGGTTTTGTGCC{AG}gt............. : 63746 23 : ntron 2 >>>> {o}GlyLeuAlaGlyThr >>>> Target Intron 3 > : 28 9 bp {!}|||||| !..! ! 16446 bp ++{g}GlyLeuIleSerGln++ 63747 : ............ag{G}GGCCTCATTTCTCAAgt...................... : 108551 29 : >>> MetCysAlaSer >>>> Target Intron 4 >>>> ArgAspAspT : 35 ! |||:!!..! 13025 bp !!||| | ++ThrCysSerGlu++ ++GlyAspProT 108552 : ...agACCTGCTCAGAGgt.........................agGGCGATCCGT : 138041 36 : rpArgCysAlaArgSer{Me} >>>> Target Intron 5 >>>> {t}His : 42 |||||||| !!! !|||{ } 8520 bp {!}!!. rpArgCysProThrSer{Tr}++ ++{p}Gln 138042 : GGCGTTGTCCCACGTCG{TG}gt.........................ag{G}CAG : 146582 43 : GluPheSerAla{Ly} >>>> Target Intron 6 >>>> {s}AspIleAs : 50 ..! ! !!|||{!:} 25263 bp {!} !|| ArgAsnProAla{Ar}++ -+{g}ArgCysAs 146583 : AGGAACCCAGCC{AG}gt.........................cg{G}CGATGTGA : 171869 51 : pGlyHisMetValAsnLeuAspLysTyrArgGlyPheValCysIleValThrAsnV : 69 | ! ! ! !!!.! ! !! ! !!!:!|||.!.|||||| ! !||||||: pLysLysAlaTrpLysSerHisLeuAsnLysGlyMetValCysProProThrAsnL 171870 : CAAGAAAGCGTGGAAGTCACATTTGAATAAAGGGATGGTGTGTCCACCCACAAATC : 171926 70 : alAlaSerGlnUnk{Gl} >>>> Target Intron 7 >>>> {y}LysThr : 76 !!! !|||!:: {||} 52271 bp {|}... !! euGluSerArgLeu{Gl}++ ++{y}SerPro 171927 : TTGAGTCTCGTTTG{GG}gt.........................ag{T}TCTCCT : 224218 77 : GluValAsnTyrThrGlnLeuValAspLeuHisAlaArgTyrAla >>>> Targ : 92 |||:!!!..!::|||!:!||| ! ! !.!.||| !! !||| GluIleArgTrpThrArgLeuTrpLysAlaGluAlaCysGlyAla++ 224219 : GAAATCAGATGGACACGGCTGTGGAAAGCTGAGGCTTGTGGTGCGgt......... : 224268 93 : et Intron 8 >>>> GluCysGlyLeu{Ar} >>>> Target Intron 9 : 96 73561 bp |||||||||! !{||} 86752 bp ++GluCysGlyCys{Ar}+- 224269 : ................agGAATGTGGGTGC{CG}gc.................... : 297843 97 : >>>> {g}IleLeuAlaPheProCysAsnGln{Ph} >>>> Target Intr : 105 {|}:!!! ! ! ||||||...|||{ } 104383 ++{g}ValProArgGluProCysGlyGln{Ar}++ 297844 : .....ag{G}GTCCCCAGAGAGCCCTGTGGACAG{AG}gt................ : 384622 106 : on 10 >>>> {e}GlyLysGlnGluProGlySerAsnGluGluIleLysGluPh : 119 bp { }|||...... ||| ...... ...:::... .. ++{g}GlySerSerProProArgAspGlyThrHisValSerCysIl 384623 : ..........ag{g}ggatcttccccacccagggatggaacacacgtctcctgtat : 489043 120 : eAlaAla{G} >>>> Target Intron 11 >>>> {ly}TyrAsnValLys : 126 . |||{|} 5597 bp {||}|||...:!!! ! eLeuAla{G}++ ++{ly}TyrLysIleThr 489044 : tctggca{g}gt..........................ag{gt}tataaaaTCACA : 494661 127 : PheAspMetPheSerLysIleCysVal >>>> Target Intron 12 >>>> : 136 ||| ||| |||||| 59944 bp PheArgGlyPheProGlyGlyCysVal++ + 494662 : TTccgggggttcccaggtggttgtgtggt..........................a : 554633 137 : AsnGlyAspAsp{A} >>>> Target Intron 13 >>>> {la}HisPro : 142 |||!!:|||{ } 7588 bp { !}|||||| +ValGlyGluAsp{A}++ ++{sn}HisPro 554634 : gGTGGGAGAAGAC{A}gt..........................ag{AC}CATCCC : 562241 143 : LeuTrpLysTrpMetLysIleGlnPro >>>> Target Intron 14 >>>> : 152 ! !|||!:!||| !|||! ! 104387 bp CysTrpArgTrpProLeuAlaGlnLeu++ + 562242 : TGTTGGAGATGGCCTCTTGCCCAGCTGgt..........................a : 666656 153 : >>>> Target Intron 15 >>>> LysGlyLysGlyIleLeu{G} >> : 158 21531 bp ! !|||...|||:!!! !{|} +++ ++ThrGlySerGlyValGln{G}++ 666657 : ggt..........................agACAGGCTCTGGAGTACAA{G}gt.. : 688210 159 : >> Target Intron 16 >>>> {ly}AsnAla >>>> Target Intron : 161 150757 bp {||}::! ! 24800 bp ++{ly}SerLys-+ 688211 : ........................ag{GA}TCCAAGat.................. : 838975 162 : 17 >>>> IleLysTrpAsnPhe{Th} >>>> Target Intron 18 >>> : 166 ||||||!::|||! !{! } 32499 bp ++IleLysPheAsnSer{Ar}++ 838976 : ........agATAAAATTCAATTCC{AG}gt......................... : 863792 167 : > {r}Lys >>>> Target Intron 19 >>>> PheLeuIleAspLysAs : 173 {!}::! 10324 bp ||||||||| ::!!: ++{g}Arg++ ++PheLeuIleLeuArgSe 863793 : .ag{G}CGGgt..........................agTTCCTCATCCTGCGAAG : 906632 174 : nGly{C} >>>> Target Intron 20 >>>> {ys}ValValLysArg{T} : 180 !!.!{ } 31681 bp { }|||::: |||{|} rAla{V}++ ++{al}ValMetTyrArg{T} 906633 : CGCG{G}gt..........................ag{ta}gtcatgtacaga{t} : 938334 181 : >>>> Target Intron 21 >>>> {yr}GlyProMetGluGluProLeuV : 188 48485 bp {||}||| !!:!: ! ||| !: ++ ++{yr}GlySerLeuProValProAlaL 938335 : gt..........................ag{AT}GGTTCTCTTCCTGTCCCAGCAC : 986843 189 : al >>>> Target Intron 22 >>>> >>>> Target Intron 23 : 189 !! 24531 bp 100771 bp eu++ ++++ 986844 : TCgt..........................aggt...................... : 1011379 190 : >>>> IleGluLysAspLeuProHisTyr : 196 :!!... !..! !||||||!:! ++ValSerProSerAlaProHisPhe 1011380 : ....agGTAAGTCCGTCTGCGCCACATTTC : 1112171 vulgar: SPP00000007_1.0 1 196 . NIVO01030317.1:subseq(555646,1182752) 17813 1112171 + 131 M 11 33 S 0 1 5 0 2 I 0 45864 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 44785 3 0 2 S 1 1 M 5 15 5 0 2 I 0 16442 3 0 2 M 4 12 5 0 2 I 0 13021 3 0 2 M 9 27 S 0 2 5 0 2 I 0 8516 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 25259 3 0 2 S 1 1 M 26 78 S 0 2 5 0 2 I 0 52267 3 0 2 S 1 1 M 17 51 5 0 2 I 0 73557 3 0 2 M 4 12 S 0 2 5 0 2 I 0 86748 3 0 2 S 1 1 M 8 24 S 0 2 5 0 2 I 0 104379 3 0 2 S 1 1 M 16 48 S 0 1 5 0 2 I 0 5593 3 0 2 S 1 2 M 13 39 5 0 2 I 0 59940 3 0 2 M 4 12 S 0 1 5 0 2 I 0 7584 3 0 2 S 1 2 M 11 33 5 0 2 I 0 104383 3 0 2 5 0 2 I 0 21527 3 0 2 M 6 18 S 0 1 5 0 2 I 0 150753 3 0 2 S 1 2 M 2 6 5 0 2 I 0 24796 3 0 2 M 5 15 S 0 2 5 0 2 I 0 32495 3 0 2 S 1 1 M 1 3 5 0 2 I 0 10320 3 0 2 M 7 21 S 0 1 5 0 2 I 0 31677 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 48481 3 0 2 S 1 2 M 8 24 5 0 2 I 0 24527 3 0 2 5 0 2 I 0 100767 3 0 2 M 8 24 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 17814 1112171 131 + . gene_id 10 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 17814 17847 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 17814 17847 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 17848 17849 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 17848 63715 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 63714 63715 . + . intron_id 0 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 63716 63743 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 63716 63743 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 63744 63745 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 63744 108532 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 108531 108532 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 108533 108548 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 108533 108548 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 108549 108550 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 108549 124994 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 124993 124994 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 124995 125006 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 124995 125006 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 125007 125008 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 125007 138031 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 138030 138031 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 138032 138060 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 138032 138060 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 138061 138062 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 138061 146580 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146579 146580 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146581 146598 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146581 146598 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146599 146600 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146599 171861 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171860 171861 . + . intron_id 5 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171862 171942 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171862 171942 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171943 171944 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171943 224213 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 224212 224213 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 224214 224265 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 224214 224265 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 224266 224267 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 224266 297826 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297825 297826 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297827 297840 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297827 297840 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297841 297842 . + . intron_id 9 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297841 384592 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 384591 384592 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 384593 384619 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 384593 384619 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 384620 384621 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 384620 489002 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 489001 489002 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 489003 489052 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 489003 489052 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 489053 489054 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 489053 494649 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 494648 494649 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 494650 494690 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 494650 494690 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 494691 494692 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 494691 554634 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 554633 554634 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 554635 554647 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 554635 554647 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 554648 554649 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 554648 562235 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 562234 562235 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 562236 562270 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 562236 562270 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 562271 562272 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 562271 666657 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 688188 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 688187 688188 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 688189 688207 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 688189 688207 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 688208 688209 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 688208 838964 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 838963 838964 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 838965 838972 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 838965 838972 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 838973 838974 . + . intron_id 17 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 838973 863772 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 896288 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 896287 896288 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 896289 896292 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 896289 896292 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 896293 896294 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 896293 906616 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 906615 906616 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 906617 906638 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 906617 906638 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 906639 906640 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 906639 938319 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 938318 938319 . + . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 938320 938334 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 938320 938334 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 938335 938336 . + . intron_id 21 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 938335 986819 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 986818 986819 . + . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 986820 986845 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 986820 986845 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 986846 986847 . + . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 986846 1011376 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1112147 . + . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1112148 1112171 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1112148 1112171 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 17814 1112171 131 + . alignment_id 10 ; Query SPP00000007_1.0 ; Align 17814 2 33 ; Align 63718 14 24 ; Align 108534 23 15 ; Align 124995 28 12 ; Align 138032 32 27 ; Align 146582 42 15 ; Align 171863 48 78 ; Align 224215 75 51 ; Align 297827 92 12 ; Align 384594 97 24 ; Align 489004 106 48 ; Align 494652 123 39 ; Align 554635 136 12 ; Align 562238 141 33 ; Align 688189 152 18 ; Align 838967 159 6 ; Align 863773 161 15 ; Align 896290 167 3 ; Align 906617 168 21 ; Align 938322 176 12 ; Align 986822 181 24 ; Align 1112148 189 24 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 129 Query range: 1 -> 168 Target range: 1826 -> 1156117 2 : SerLeuGlyArgLeuCysArgLeu{L} >>>> Target Intron 1 >>>> : 10 .!!|||||||||:!!|||! ! !{ } 144529 bp GlyLeuGlyArgIleCysThrLys{G}+- ++ 1827 : GGGCTAGGAAGGATCTGTACAAAA{G}ga.........................ag : 146379 11 : {eu}LysProAlaLeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAlaGlyThr : 28 { !}!:! !||||||:!! !|||!.!! ! ! !|||||| !::!.!! ! {ly}ArgAspAlaLeuIleProGlyValCysLeuGlnProGlyThrSerSerGln+ 146380 : {GA}AGGGATGCCCTCATACCTGGGGTGTGCTTGCAACCTGGGACCAGCAGCCAGg : 146434 29 : >>>> Target Intron 2 >>>> MetCysAlaSerArgAspAspTrpArgC : 37 151393 bp |||!.!!!!|||.!.||| !!|||! + ++GluCysGlyCysArgGlnAspArgArgL 146435 : t.........................agGAATGTGGGTGCCGGCAGGACAGGCGAT : 297854 38 : ys >>>> Target Intron 3 >>>> AlaArgSerMetHisGluPheSerA : 46 29081 bp :!! !!..!:!!|||! !.!.||| eu++ ++Ser***GlnLeuHisValLeuSerP 297855 : TGgt.........................agTCATGACAACTGCATGTACTGAGTC : 326962 47 : laLysAspIleAsp >>>> Target Intron 4 >>>> GlyHisMetValA : 55 !!||| :!!.!. 157529 bp ||| :::: roLysArgValGln++ ++GlySerThrLeuH 326963 : CTAAGAGGGTTCAAgt.........................agggatcgaccctgc : 484518 56 : snLeuAspLysTyrArg >>>> Target Intron 5 >>>> GlyPheValC : 64 ::||| :::||| 116924 bp |||... isLeuLeuProTrpArg++ ++GlyIlePheP 484519 : atCTCTTACcgtggcgggt.........................aggggatcttcc : 601469 65 : ysIleVal >>>> Target Intron 6 >>>> >>>> Target Intro : 67 ||| 65180 bp 75639 bp roIleGln++ ++++ 601470 : caatccaggt.........................aggt................. : 666660 68 : n 7 >>>> ThrAsnVal{A} >>>> Target Intron 8 >>>> {la}S : 71 |||||||||{!} 101956 bp {.!}| ++ThrAsnVal{G}-+ ++{ly}S 666661 : ........agACTAacgta{g}tt.........................ag{GA}T : 844265 72 : erGlnUnkGlyLysThrGlu >>>> Target Intron 9 >>>> ValAsnT : 80 |||||.....!||| !:!! 19487 bp :!!!!.! erGlnThrSerLysLeuGln++ ++IleLysP 844266 : CTCAGACATCCAAACTCCAGgt.........................agATAAAAT : 863779 81 : yrThrGln{Le} >>>> Target Intron 10 >>>> {u}ValAspLeuHi : 87 :!!.!...{ } 4896 bp {!} ... .. heAsnSer{Ar}++ ++{g}ArgSerSerAr 863780 : TCAATTCC{AG}gt..........................ag{g}agatcttcccg : 868696 88 : sAlaArgTyrAlaGluCysGlyLeuArgIleLeuAlaPhePro >>>> Target : 102 . ||| !.!...||| ||||||:::||| ::: 27 gProArgGlyGlyAsnCysLeuLeuArgLeuLeuHisTrpGln++ 868697 : accgaggggtGGAAactgtctcttacgtctcctgcactggcaggt........... : 868743 103 : Intron 11 >>>> Cys{A} >>>> Target Intron 12 >>>> {sn : 103 548 bp ...{.} 84784 bp {!. ++Ala{G}++ ++{lu 868744 : ...............agGCG{G}gt..........................ag{aa : 981077 104 : }GlnPheGlyLysGlnGluPro >>>> Target Intron 13 >>>> >> : 111 }... |||::::::...||| 30277 bp }SerHisGlyGlnArgSerPro++ ++++ 981078 : }tcccatggacagaggagtccggt..........................aggt.. : 1011379 112 : >> Target Intron 14 >>>> GlySerAsnGluGluIleLysGluPheAla : 120 86371 bp ||||||||| ...... ...::: ++GlySerAsnLeuSerPheLeuHisTrpGln 1011380 : ........................agggatcgaacctgagtttcctacattggcag : 1097775 121 : >>>> Target Intron 15 >>>> >>>> Target Intron 16 >> : 121 14370 bp 7352 bp ++ ++++ 1097776 : gt..........................aggt........................ : 1112150 122 : >> AlaGlyTyrAsnValLysPheAspMetPheSerLysIleCysValAsnGlyA : 138 ...!.! ! ! ! ! !|||:!! !!!! !:!! !!:!!||| !!! -+GlyAlaValLeuGlyTyrAsnAspValArg***ThrValGlyIleAsnCysG 1112151 : ..tgggtGCTGTCTTGGGGTATAATGACGTGAGGTGAACAGTAGGTATTAACTGTG : 1119551 139 : spAspAlaHisProLeuTrp{L} >>>> Target Intron 17 >>>> {ys : 145 .!. !||||||||||||{ } 12750 bp { lyGlnLeuHisProLeuTrp{A}-+ ++{la 1119552 : GACAACTTCATCCGCTTTGG{G}tt..........................ag{CT : 1132323 146 : }TrpMetLysIleGlnProLysGlyLys{G} >>>> Target Intron 18 > : 155 }|||! :!! !||| !! !||| !{|} 15816 bp }TrpThrGluAlaGlnAlaProGlyLeu{G}++ 1132324 : }TGGACTGAGGCTCAGGCCCCGGGCCTG{G}gt....................... : 1132355 156 : >>> {ly}IleLeuGlyAsnAla{I} >>>> Target Intron 19 >>>> : 161 {||}||| !|||!.. !!{:} 7908 bp ++{ly}IleArgGlyArgPro{V}++ + 1132356 : ...ag{GG}ATAAGGGGGAGACCA{G}gt..........................a : 1156093 162 : {le}LysTrpAsnPheThrLysPhe : 168 {!!} |||:::... :::||| +{al}LeuTrpSerLeuProArgPhe 1156094 : g{tc}ctgtggtcactgccaaggttt : 1156117 vulgar: SPP00000007_1.0 1 168 . NIVO01030317.1:subseq(555646,1182752) 1826 1156117 + 129 M 8 24 S 0 1 5 0 2 I 0 144525 3 0 2 S 1 2 M 17 51 5 0 2 I 0 151389 3 0 2 M 10 30 5 0 2 I 0 29077 3 0 2 M 13 39 5 0 2 I 0 157525 3 0 2 M 10 30 5 0 2 I 0 116920 3 0 2 M 6 18 5 0 2 I 0 65176 3 0 2 5 0 2 I 0 75635 3 0 2 M 3 9 S 0 1 5 0 2 I 0 101952 3 0 2 S 1 2 M 7 21 5 0 2 I 0 19483 3 0 2 M 5 15 S 0 2 5 0 2 I 0 4892 3 0 2 S 1 1 M 18 54 5 0 2 I 0 27544 3 0 2 M 1 3 S 0 1 5 0 2 I 0 84780 3 0 2 S 1 2 M 7 21 5 0 2 I 0 30273 3 0 2 5 0 2 I 0 86367 3 0 2 M 10 30 5 0 2 I 0 14366 3 0 2 5 0 2 I 0 7348 3 0 2 M 24 72 S 0 1 5 0 2 I 0 12746 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 15812 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 7904 3 0 2 S 1 2 M 7 21 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 1827 1156117 129 + . gene_id 11 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1827 1851 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1827 1851 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1852 1853 . + . intron_id 1 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1852 146380 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146379 146380 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146381 146433 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146381 146433 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146434 146435 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146434 297826 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297825 297826 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297827 297856 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297827 297856 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297857 297858 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297857 326937 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 326936 326937 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 326938 326976 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 326938 326976 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 326977 326978 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 326977 484505 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 484504 484505 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 484506 484535 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 484506 484535 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 484536 484537 . + . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 484536 601459 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 666657 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 742296 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 742295 742296 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 742297 742306 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 742297 742306 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 742307 742308 . + . intron_id 8 ; splice_site "tt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 742307 844262 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 844261 844262 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 844263 844285 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 844263 844285 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 844286 844287 . + . intron_id 9 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 844286 863772 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 868685 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 868684 868685 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 868686 868740 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 868686 868740 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 868741 868742 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 868741 896288 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 896287 896288 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 896289 896292 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 896289 896292 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 896293 896294 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 896293 981076 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 981075 981076 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 981077 981099 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 981077 981099 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 981100 981101 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 981100 1011376 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1097747 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1097746 1097747 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1097748 1097777 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1097748 1097777 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1097778 1097779 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1097778 1112147 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1119499 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1119498 1119499 . + . intron_id 15 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1119500 1119572 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1119500 1119572 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1119573 1119574 . + . intron_id 17 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1119573 1132322 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1132321 1132322 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1132323 1132352 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1132323 1132352 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1132353 1132354 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1132353 1148168 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1148167 1148168 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1148169 1148186 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1148169 1148186 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1148187 1148188 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1148187 1156094 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1156093 1156094 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1156095 1156117 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1156095 1156117 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 1827 1156117 129 + . alignment_id 11 ; Query SPP00000007_1.0 ; Align 1827 2 24 ; Align 146383 11 51 ; Align 297827 28 30 ; Align 326938 38 39 ; Align 484506 51 30 ; Align 601460 61 18 ; Align 742297 67 9 ; Align 844265 71 21 ; Align 863773 78 15 ; Align 868687 84 54 ; Align 896289 102 3 ; Align 981079 104 21 ; Align 1097748 111 30 ; Align 1119500 121 72 ; Align 1132325 146 27 ; Align 1148171 156 15 ; Align 1156097 162 21 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 127 Query range: 13 -> 195 Target range: 21800 -> 1070677 14 : LeuLeuCysGlyAlaLeuAlaAlaPro >>>> Target Intron 1 >>>> : 23 ||||||||||||||| !!.!.!! ! 10384 bp LeuLeuCysGlyAlaGlyGlyThrLys++ ++ 21801 : CTCCTCTGTGGAGCTGGGGGGACCAAAgt.........................ag : 32210 24 : GlyLeuAlaGlyThrMetCysAlaSerArgAspAspTrpArgCysAla >>>> T : 39 !.!! !!.!|||:!!:!!|||:::...:::... ||| ||| AlaSerValGlySerLeuCysSerGlyGlnGlnGlyTrpGlyCysArg+- 32211 : GCTTCAGTGGGCTCATTGTGCAGtgggcagcaggggtgggggtgcagggg...... : 32262 40 : arget Intron 2 >>>> ArgSerMetHisGluPheSerAlaLysAspIleAs : 50 122490 bp |||.!!:!:||| ! !! ! ! ! ! ++ArgGlyLeuHisProHisPheLeuLeuThrProLe 32263 : ...................agAGGGGGCTCCATCCACACTTCCTCCTCACCCCCCT : 154783 51 : pGlyHisMetValAsnLeu >>>> Target Intron 3 >>>> Asp{L} : 58 !||||||:!::!!.!.:!! 16257 bp { } uGlyHisLeuIleGlnMet++ ++Pro{G}++ 154784 : CGGGCACCTTATCCAGATGgt.........................agCCA{G}gt : 171065 59 : >>>> Target Intron 4 >>>> {ys}TyrArgGlyPheValCysIle >> : 66 46012 bp { !} ||| !|||:!!|||:!: ++{ly}LeuArgMetPheLeuCysVal+- 171066 : .........................ag{gg}CTGAGGATGTTCCTGTGTGTGga.. : 217102 67 : >> Target Intron 5 >>>> ValThrAsnValAlaSerGln >>>> Tar : 73 75602 bp ||| !||| ! !!!!||| ++ValLeuAsnTyrIleThrGln++ 217103 : .......................aggtgctAAATTATATCACACAGgt........ : 292725 74 : get Intron 6 >>>> UnkGlyLysThrGluValAsnTyrThrGlnLeuVal{ : 85 32987 bp !!!. !!||||||.!.! ! |||...{ ++GlyProAsnProGluValGluSerProIleLeuAla{ 292726 : .................agGGTCCCAATCCAGAGGTTGAATCTcccatattggca{ : 325746 86 : A} >>>> Target Intron 7 >>>> {sp}LeuHisAlaArgTyrAlaGlu : 92 |} 85524 bp {||}:::::: :::|||... A}++ ++{sp}MetTyrMetGlnTyrGlyVal 325747 : g}gt.........................ag{at}atgtacatgcaatatggtgtg : 411291 93 : CysGlyLeuArgIleLeuAlaPheProCysAsnGln >>>> Target Intron : 105 ||| ::: ||||||... 67966 bp CysValTyrMetThrTyrIleTyrThrCysAsnMet++ 411292 : tgtgtatatatgacatatatatatacgtgcaatatggt.................. : 411332 106 : 8 >>>> PheGlyLys >>>> Target Intron 9 >>>> GlnGluPro : 110 .!.|||||| 23271 bp ! ||| ++LeuGlyLys++ ++ProProPro 411333 : .......agCTGGGAAAGgt.........................agCCTCCCCCA : 502582 111 : {Gl} >>>> Target Intron 10 >>>> {y}SerAsnGluGluIleLysG : 118 {||} 23655 bp {|}||||||...!!:.!! ! {Gl}++ ++{y}SerAsnArgAspPheLeuA 502583 : {GG}gt..........................ag{C}TCGAACCGTGACTTCCTTG : 526261 119 : luPheAlaAlaGlyTyrAsnValLysPhe{A} >>>> Target Intron 11 : 128 !: ! :!! !!! !:!!::!:!!! !.!!{|} 9031 bp spValSerProAspHisSerLeuMetLeu{A}++ 526262 : ACGTATCCCCAGATCACTCTCTCATGCTT{G}gt...................... : 526294 129 : >>>> {sp}Met{Ph} >>>> Target Intron 12 >>>> {e}SerLys : 132 {||} {! } 38287 bp {!}|||:!! ++{sp}Ser{Se}++ ++{r}SerGln 526295 : ....ag{at}tcc{tc}gt..........................ag{C}TCTCAA : 573621 133 : IleCys{Va} >>>> Target Intron 13 >>>> {l}AsnGlyAspAspA : 140 :!!|||{..} 27828 bp {!}............: LeuCys{Th}++ ++{r}GlySerSerGlnS 573622 : CTCTGT{AC}gt..........................ag{g}ggatcttcccaat : 601473 141 : la{Hi} >>>> Target Intron 14 >>>> {s}ProLeuTrpLys >>> : 146 ::{..} 104318 bp {.}|||||||||::! er{Ar}++ ++{g}ProLeuTrpArg++ 601474 : cc{ag}gt..........................ag{G}CCTCTCTGGCGGgt... : 705811 147 : > Target Intron 15 >>>> TrpMetLysIle >>>> Target Intro : 150 151474 bp |||:!!:!! 123782 b ++TrpLeuGluGlu++ 705812 : .......................agTGGCTGGAAGAGgt................. : 857297 151 : n 16 >>>> GlnProLysGlyLysGlyIle{Le} >>>> Target Intron : 157 p ...||| :::|||:::{! } 11308 bp ++AsnProMetAspArgGlyVal{Ar}++ 857298 : .........agaatcccatggacagaggagtc{cg}gt.................. : 981102 158 : 17 >>>> {u}GlyAsnAlaIleLysTrpAsnPheThrLysPheLeuIleAspL : 172 {!}|||:::::: ::: ||| ... ! !! !! ++{g}GlySerSerArgProArgAspGlnThrHisIleCysCysValT 981103 : ........ag{g}ggatcttcccgacccagggatcaaactcacatttgCTGTGTCA : 992451 173 : ysAsnGlyCysVal >>>> Target Intron 18 >>>> >>>> Targe : 177 !||| 18911 bp 3 hrLeuHisCysGln++ ++++ 992452 : CCTTGCATtgccaggt..........................aggt.......... : 1011379 178 : t Intron 19 >>>> ValLysArgTyrGlyProMetGluGluProLeuValIl : 189 0163 bp :!! ! ! ! !..!|||:!!!!:..!||| !..!:! ++LeuHisLeuGlyAsnProValAspArgProGlyThrVa 1011380 : ................agCTCCACCTCGGTAACCCAGTGGACAGGCCAGGCACTGT : 1041576 190 : eGlu{L} >>>> Target Intron 20 >>>> {ys}AspLeuProHis : 195 :|||{ } 29081 bp { !}|||||||||||| lGlu{G}++ ++{ly}AspLeuProHis 1041577 : GGAG{G}gt..........................ag{gg}gatcttccccac : 1070677 vulgar: SPP00000007_1.0 13 195 . NIVO01030317.1:subseq(555646,1182752) 21800 1070677 + 127 M 9 27 5 0 2 I 0 10380 3 0 2 M 16 48 5 0 2 I 0 122486 3 0 2 M 18 54 5 0 2 I 0 16253 3 0 2 M 1 3 S 0 1 5 0 2 I 0 46008 3 0 2 S 1 2 M 7 21 5 0 2 I 0 75598 3 0 2 M 7 21 5 0 2 I 0 32983 3 0 2 M 12 36 S 0 1 5 0 2 I 0 85520 3 0 2 S 1 2 M 19 57 5 0 2 I 0 67962 3 0 2 M 3 9 5 0 2 I 0 23267 3 0 2 M 3 9 S 0 2 5 0 2 I 0 23651 3 0 2 S 1 1 M 16 48 S 0 1 5 0 2 I 0 9027 3 0 2 S 1 2 M 1 3 S 0 2 5 0 2 I 0 38283 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 27824 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 104314 3 0 2 S 1 1 M 4 12 5 0 2 I 0 151470 3 0 2 M 4 12 5 0 2 I 0 123778 3 0 2 M 7 21 S 0 2 5 0 2 I 0 11304 3 0 2 S 1 1 M 19 57 5 0 2 I 0 18907 3 0 2 5 0 2 I 0 30159 3 0 2 M 14 42 S 0 1 5 0 2 I 0 29077 3 0 2 S 1 2 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 21801 1070677 127 + . gene_id 12 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 21801 21827 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 21801 21827 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 21828 21829 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 21828 32211 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 32210 32211 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 32212 32259 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 32212 32259 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 32260 32261 . + . intron_id 2 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 32260 154749 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 154748 154749 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 154750 154803 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 154750 154803 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 154804 154805 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 154804 171060 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 217076 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 217075 217076 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 217077 217099 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 217077 217099 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 217100 217101 . + . intron_id 5 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 217100 292701 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 292700 292701 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 292702 292722 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 292702 292722 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 292723 292724 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 292723 325709 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 325708 325709 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 325710 325746 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 325710 325746 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 325747 325748 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 325747 411270 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 411269 411270 . + . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 411271 411329 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 411271 411329 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 411330 411331 . + . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 411330 479295 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 479294 479295 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 479296 479304 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 479296 479304 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 479305 479306 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 479305 502575 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 502574 502575 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 502576 502586 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 502576 502586 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 502587 502588 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 502587 526241 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 526240 526241 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 526242 526291 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 526242 526291 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 526292 526293 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 526292 535322 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 535321 535322 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 535323 535329 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 535323 535329 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 535330 535331 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 535330 573616 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 573615 573616 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 573617 573631 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 573617 573631 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 573632 573633 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 573632 601459 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 705795 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 705794 705795 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 705796 705808 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 705796 705808 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 705809 705810 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 705809 857282 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 857281 857282 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 857283 857294 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 857283 857294 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 857295 857296 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 857295 981076 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 981075 981076 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 981077 981099 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 981077 981099 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 981100 981101 . + . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 981100 992407 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 992406 992407 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 992408 992465 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 992408 992465 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 992466 992467 . + . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 992466 1011376 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1041539 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1041538 1041539 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1041540 1041582 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1041540 1041582 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1041583 1041584 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1041583 1070663 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1070662 1070663 . + . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1070664 1070677 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1070664 1070677 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 21801 1070677 127 + . alignment_id 12 ; Query SPP00000007_1.0 ; Align 21801 14 27 ; Align 32212 23 48 ; Align 154750 39 54 ; Align 171061 57 3 ; Align 217079 59 21 ; Align 292702 66 21 ; Align 325710 73 36 ; Align 411273 86 57 ; Align 479296 105 9 ; Align 502576 108 9 ; Align 526243 112 48 ; Align 535325 129 3 ; Align 573618 131 12 ; Align 601461 136 15 ; Align 705797 142 12 ; Align 857283 146 12 ; Align 981077 150 21 ; Align 992409 158 57 ; Align 1041540 177 42 ; Align 1070666 192 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 132 Query range: 0 -> 194 Target range: 154537 -> 1162664 1 : MetSerLeuGlyArgLeuCysArgLeuLeu{L} >>>> Target Intron 1 : 11 :!!|||! !!.!|||||||||!:!! !! !{ } 23181 bp LeuSerGlnAlaArgLeuCysLysProPro{A}++ 154538 : CTGAGCCAGGCCCGCTTGTGCAAGCCCCCG{G}gt..................... : 154571 12 : >>>> {ys}ProAlaLeuLeuCysGlyAlaLeu{Al} >>>> Target Intr : 20 { }|||...|||||| ... |||{::} 76611 b ++{la}ProCysLeuLeuProAlaProLeu{Se}++ 154572 : ....ag{CC}CCttgcctcctgcctgcccccctC{AG}gt................ : 177780 21 : on 2 >>>> {a}AlaPro{G} >>>> Target Intron 3 >>>> {ly} : 23 p {!}||||||{|} 66713 bp {||} ++{r}AlaPro{G}++ ++{ly} 177781 : .........ag{T}GCTCCA{G}gt.........................ag{GC} : 321110 24 : LeuAlaGlyThrMetCysAlaSerArgAspAspTrpArgCysAlaArgSerMetHi : 42 ||| !||| !:!!||| !!!!!!! ! ||| !! !||| ! !! !! || LeuLysGlyHisLeuCys******Ser***AspMetIleCysLeuCysProSerHi 321111 : CTGAAAGGCCACTTGTGTTGATGAAGTTAAGATATGATTTGCCTTTGCCCCTCTCA : 321166 43 : sGluPhe{S} >>>> Target Intron 4 >>>> {er}AlaLysAspIleA : 50 |! !!:!{.} 1277 bp {!!} !!..||| !. sGlyTyr{G}++ ++{ly}LeuSerAspAlaS 321167 : TGGATAT{G}gt.........................ag{GG}CTGAGTGATGCTT : 322467 51 : sp{Gl} >>>> Target Intron 5 >>>> {y}HisMetValAsnLeuAsp : 57 .!{||} 71477 bp {|} !:!!|||.!.:!! ! er{Gl}++ ++{y}CysLeuValGluIleThr 322468 : CC{GG}gt.........................ag{G}TGTCTGGTAGAAATCACC : 393965 58 : {L} >>>> Target Intron 6 >>>> {ys}TyrArgGlyPheValCys : 65 {|} 718 bp {||}|||!:!|||!:!:!!||| {L}++ ++{ys}TyrGlnGlyTyrIleCys++ 393966 : {A}gt.........................ag{AA}TATCAGGGGTATATCTGTgt : 394707 66 : >>>> Target Intron 7 >>>> IleValThr{As} >>>> Target In : 68 42208 bp :!: !|||{!.} 164534 ++LeuGlnThr{Ar}++ 394708 : .........................agCTGCAAACC{AG}gt.............. : 436928 69 : tron 8 >>>> {n}ValAlaSerGlnUnk{Gl} >>>> Target Intron : 74 bp {.} :::||||||...{.!} 3954 bp ++{g}GlySerSerGlnSer{Se}++ 436929 : ...........ag{g}ggatcttcccaatcc{ag}gt................... : 601480 75 : 9 >>>> {y}LysThrGluValAsnTyrThrGlnLeuValAspLeuHis{Al} : 88 {!}::!..!.!.! !!:!!:! !:!! ! !..!||||||{!.} ++{r}ArgValHisGluSerPheLeuGluGluCysSerLeuHis{Gl}++ 601481 : ......ag{C}CGAGTCCATGAAAGCTTCTTGGAGGAATGCAGCCTTCAT{GG}gt : 605474 89 : >>>> Target Intron 10 >>>> {a}ArgTyrAlaGluCysGlyLeuArgI : 97 10450 bp {!} :::... |||||| ! ++{y}GlyTrpGlyIleLeuGlyLeuSerG 605475 : ..........................ag{g}ggctgggggataCTGGGTCTGTCGG : 615949 98 : leLeuAla >>>> Target Intron 11 >>>> >>>> Target Intr : 100 |||||| 50700 bp 15902 b luLeuAla++ ++++ 615950 : AGTTGGCTgt..........................aggt................ : 666660 101 : on 12 >>>> PhePro{Cy} >>>> Target Intron 13 >>>> {s}A : 103 p ||| !{||} 28669 bp {|}! ++PheLeu{Cy}++ ++{s}A 666661 : ..........agttccTA{TG}gt..........................ag{C}A : 711238 104 : snGlnPheGlyLysGln{G} >>>> Target Intron 14 >>>> {lu}Pr : 110 ....! |||!:!:!!{!} 103084 bp {!:} rgMetThrGlyArgGlu{A}++ ++{sp}Gl 711239 : GGATGACAGGGAGGGAA{G}gt..........................ag{ac}ca : 814343 111 : oGlySerAsnGluGluIleLysGluPheAlaAlaGly{T} >>>> Target In : 123 ||||||||| ...::: ... ||| { } 49390 nGlySerAsnProSerLeuMetSerProAlaLeuThr{A}++ 814344 : gggatcaaacccgagtctcatgtctcctgcactgaca{g}gt.............. : 814385 124 : tron 15 >>>> {yr}AsnValLysPheAsp >>>> Target Intron 16 : 129 bp {!!}!!.:!!:!!|||.!. 147587 bp ++{sp}LysIleGlnPheGln++ 814386 : ............ag{AT}AAAATTCAATTCCAGgt..................... : 863792 130 : >>>> >>>> Target Intron 17 >>>> MetPheSerLysIleCysV : 135 82006 bp !!!!!...!!|||: ++++ ++GlyAspThrSerPheCysL 863793 : .....aggt..........................agGGAGACACCAGCTTCTGCC : 1093401 136 : alAsnGlyAspAspAlaHisProLeuTrpLysTrpMet{L} >>>> Target I : 148 !!.!.||| !|||!.! ! !!:!!||| |||! {!} 430 euGluGlyCysAspValAlaSerValTrpGlyTrpAsn{A}++ 1093402 : TGGAAGGATGTGACGTGGCCTCGGTGTGGGGCTGGAAT{A}gt............. : 1093443 149 : ntron 18 >>>> {ys}IleGlnProLysGlyLysGlyIleLeu{G} >>>> : 158 7 bp {:!}|||:::|||::: ::: {|} ++{rg}IleGluProGluPheProThrLeuAla{G}++ 1093444 : .............ag{gg}atcgaacctgagtttcctacattggca{g}gt..... : 1097780 159 : Target Intron 19 >>>> {ly}AsnAlaIleLysTrpAsnPheThrLysPh : 168 22590 bp {||}:::!.! ...||| ! !!... ++{ly}SerGlyGlnSerTrpAlaGlnProSerPr 1097781 : .....................ag{GA}TCAGGACAGTCCTGGGCCCAGCCTTCCCC : 1120397 169 : eLeuIleAspLysAsnGlyCysValValLys{Ar} >>>> Target Intron : 179 ||| !..!..! !!||| !:!! {||} 5404 bp oLeuProSerSerLeuTrpCysSerIleLeu{Ar}++ 1120398 : ACTCCCATCCTCACTGTGGTGCTCCATCCTT{AG}gt................... : 1120434 180 : 20 >>>> {g}TyrGlyProMetGluGluProLeuValIleGlu{L} >>>> T : 191 {|}! !! !|||:!!! ...|||:!! !:!!...{:} ++{g}CysValProValAlaArgProValProLeuSer{A}++ 1120435 : .......ag{G}TGCGTGCCCGTGGCCCGCCCCGTGCCCCTCTCC{C}gt...... : 1125873 192 : arget Intron 21 >>>> {ys}AspLeuPro : 194 36783 bp {:!}||||||||| ++{rg}AspLeuPro 1125874 : ....................ag{gg}gatcttccc : 1162664 vulgar: SPP00000007_1.0 0 194 . NIVO01030317.1:subseq(555646,1182752) 154537 1162664 + 132 M 10 30 S 0 1 5 0 2 I 0 23177 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 76607 3 0 2 S 1 1 M 2 6 S 0 1 5 0 2 I 0 66709 3 0 2 S 1 2 M 21 63 S 0 1 5 0 2 I 0 1273 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 71473 3 0 2 S 1 1 M 6 18 S 0 1 5 0 2 I 0 714 3 0 2 S 1 2 M 6 18 5 0 2 I 0 42204 3 0 2 M 3 9 S 0 2 5 0 2 I 0 164530 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 3950 3 0 2 S 1 1 M 13 39 S 0 2 5 0 2 I 0 10446 3 0 2 S 1 1 M 11 33 5 0 2 I 0 50696 3 0 2 5 0 2 I 0 15898 3 0 2 M 2 6 S 0 2 5 0 2 I 0 28665 3 0 2 S 1 1 M 6 18 S 0 1 5 0 2 I 0 103080 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 49386 3 0 2 S 1 2 M 5 15 5 0 2 I 0 147583 3 0 2 5 0 2 I 0 82002 3 0 2 M 19 57 S 0 1 5 0 2 I 0 4303 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 22586 3 0 2 S 1 2 M 20 60 S 0 2 5 0 2 I 0 5400 3 0 2 S 1 1 M 11 33 S 0 1 5 0 2 I 0 36779 3 0 2 S 1 2 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 154538 1162664 132 + . gene_id 13 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 154538 154568 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 154538 154568 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 154569 154570 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 154569 177749 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 177748 177749 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 177750 177777 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 177750 177777 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 177778 177779 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 177778 254388 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 254387 254388 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 254389 254396 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 254389 254396 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 254397 254398 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 254397 321109 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 321108 321109 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 321110 321175 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 321110 321175 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 321176 321177 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 321176 322452 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 322451 322452 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 322453 322471 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 322453 322471 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 322472 322473 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 322472 393948 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 393947 393948 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 393949 393968 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 393949 393968 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 393969 393970 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 393969 394686 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 394685 394686 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 394687 394706 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 394687 394706 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 394707 394708 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 394707 436914 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 436913 436914 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 436915 436925 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 436915 436925 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 436926 436927 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 436926 601459 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 605431 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 605430 605431 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 605432 605473 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 605432 605473 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 605474 605475 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 605474 615923 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 615922 615923 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 615924 615957 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 615924 615957 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 615958 615959 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 615958 666657 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 682559 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 682558 682559 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 682560 682567 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 682560 682567 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 682568 682569 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 682568 711236 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 711235 711236 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 711237 711256 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 711237 711256 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 711257 711258 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 711257 814340 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 814339 814340 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 814341 814382 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 814341 814382 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 814383 814384 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 814383 863772 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 1011376 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1093382 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1093381 1093382 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1093383 1093440 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1093383 1093440 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1093441 1093442 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1093441 1097747 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1097746 1097747 . + . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1097748 1097777 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1097748 1097777 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1097778 1097779 . + . intron_id 19 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1097778 1120367 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1120366 1120367 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1120368 1120431 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1120368 1120431 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1120432 1120433 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1120432 1125835 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1125834 1125835 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1125836 1125870 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1125836 1125870 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1125871 1125872 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1125871 1162653 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1162652 1162653 . + . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1162654 1162664 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1162654 1162664 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 154538 1162664 132 + . alignment_id 13 ; Query SPP00000007_1.0 ; Align 154538 1 30 ; Align 177752 12 24 ; Align 254390 21 6 ; Align 321112 24 63 ; Align 322455 46 15 ; Align 393950 52 18 ; Align 394689 59 18 ; Align 436915 65 9 ; Align 601461 69 15 ; Align 605433 75 39 ; Align 615925 89 33 ; Align 682560 100 6 ; Align 711238 103 18 ; Align 814343 110 39 ; Align 863775 124 15 ; Align 1093383 129 57 ; Align 1097750 149 27 ; Align 1120370 159 60 ; Align 1125837 180 33 ; Align 1162656 192 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 124 Query range: 3 -> 153 Target range: 352061 -> 1172129 4 : GlyArgLeuCysArgLeuLeuLysPro{A} >>>> Target Intron 1 >>> : 13 ||| !|||||||||:!!! !||| !!{!} 26803 bp GlyPheLeuCysArgValTrpLysAla{G}++ 352062 : GGTTTCTTGTGTCGCGTGTGGAAAGCA{G}gt........................ : 352092 14 : > {la}LeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAla{G} >>>> Tar : 26 {.!}::: |||:::|||...:::|||...||||||{|} ++{ly}MetGluProGlySerLeuValSerProAlaLeuAla{G}++ 352093 : .ag{gg}atggaacccgggtctcttgtgtctcctgcattggca{g}gt........ : 378934 27 : get Intron 2 >>>> {ly}Thr{Me} >>>> Target Intron 3 >>> : 28 83698 bp {||}...{||} 69975 bp ++{ly}Val{Me}++ 378935 : .................ag{ga}gtc{at}gt........................ : 462639 29 : > {t}CysAlaSerArgAsp{As} >>>> Target Intron 4 >>>> {p : 34 {|} :::|||||| { } 18711 bp { ++{t}GlySerSerArgThr{Ar}+- ++{g 462640 : .ag{g}ggatcttcccgaacc{ag}gg.........................ag{g : 551341 35 : }TrpArgCysAlaArgSerMetHisGluPheSerAla{L} >>>> Target In : 47 }||| ||| |||::: ||| { } 50081 }TrpIleLeuTyrArgLeuSerHisGlnGlySerPro{G}++ 551342 : }tggattctttaccgcctgagccaccagggaagccca{g}gt.............. : 551381 48 : tron 5 >>>> {ys}AspIleAspGlyHis{M} >>>> Target Intron : 53 bp { !}|||::: ... { } 51537 bp ++{ly}AspLeuProAsnPro{A}++ 551382 : ...........ag{gg}gatcttcccaatcca{g}gt................... : 601480 54 : 6 >>>> {et}ValAsnLeuAspLysTyrArgGlyPheValCysIleValThrAs : 68 { } |||:::...:::::::::|||...::: |||::::: ++{la}PheAsnValSerGlnHisGlnGlyLeuLeuLeu***ValSerSe 601481 : ......ag{cc}ttcaacgtttcccagcatcagggtcttctcctatgagtcagctc : 653059 69 : nValAlaSerGlnUnkGlyLysThrGluValAsnTyrThr >>>> Target In : 82 :...:::... ... |||:!!.!.|||||| 13557 rAlaSerGlnValAlaLysGlyLeuGluLeuGlnTyrThr-+ 653060 : tgcgagtcaggtggccaaagggttggagCTTCAGTATACTtt.............. : 653103 83 : tron 7 >>>> >>>> Target Intron 8 >>>> GlnLeuValAspLe : 86 bp 168832 bp ::::::|||||||| ++++ ++ArgIleValAspLe 653104 : ...........aggt.........................agaggatagttgattt : 835502 87 : uHisAlaArgTyrAlaGluCysGly{L} >>>> Target Intron 9 >>>> : 95 ||||... ...||| { } 28243 bp uHisCysValAsnPheArgCysThr{A}-+ + 835503 : acattgtgttaatttcaggtgtaca{g}ct.........................a : 863771 96 : {eu}ArgIleLeuAlaPhe >>>> Target Intron 10 >>>> ProCys : 102 { !}!:!|||! ! ! 26934 bp ||| +{sp}LysIleGlnPheGln++ ++LysCys 863772 : g{AT}AAAATTCAATTCCAGgt..........................agaaatgt : 890727 103 : AsnGlnPheGly{Ly} >>>> Target Intron 11 >>>> {s}Gln >> : 109 !.!|||.!. !{:!} 5545 bp {!}!:! ThrGlnMetLys{Gl}++ ++{u}Arg++ 890728 : ACCCAAATGAAG{GA}gt..........................ag{G}CGGgt.. : 896295 110 : >> Target Intron 12 >>>> GluProGlySerAsnGlu >>>> Targe : 115 49713 bp !!:|||||| !!!.||| 6 ++AspProGlyHisLysGlu++ 896296 : ........................agGACCCCGGCCACAAGGAGgt.......... : 946026 116 : t Intron 13 >>>> >>>> Target Intron 14 >>>> GluIleLy : 117 5353 bp 23340 bp |||:!! ++++ ++GluValVa 946027 : ................aggt..........................agGAGGTCGT : 1034723 118 : sGluPheAlaAlaGlyTyrAsnValLysPhe >>>> Target Intron 15 > : 128 ! !||| ||| :::::: 39756 bp lThrAlaAlaArgGlyLeuSerLeuAlaVal++ 1034724 : TACGGCTGctcgcgggctttctctagctgtggt....................... : 1034758 129 : >>> AspMetPheSerLysIleCysValAsnGlyAsp{A} >>>> Target I : 139 ! !..! !.!!:!!|||||| !.!. !!! {!} 2019 ++ValGlnSerGlyGlnIleCysTrpGluArgGly{G}++ 1034759 : ...agGTCCAGAGTGGGCAGATCTGCTGGGAGAGAGGG{G}gt............. : 1074548 140 : ntron 16 >>>> {sp}AlaHis{P} >>>> Target Intron 17 >>>> : 142 3 bp {!:} !|||{ } 77347 bp ++{lu}LeuHis{*}++ 1074549 : .............ag{AG}CTGCAC{T}gt.......................... : 1094750 143 : {ro}LeuTrpLysTrpMetLysIleGlnProLysGly : 153 { !}||| |||:::...:::|||||||||||| ++{**}LeuThrGlyTrpIleSerLeuGlnProLysGly 1094751 : ag{ga}ttgactggttggatctccttgcagcccaaggga : 1172129 vulgar: SPP00000007_1.0 3 153 . NIVO01030317.1:subseq(555646,1182752) 352061 1172129 + 124 M 9 27 S 0 1 5 0 2 I 0 26799 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 83694 3 0 2 S 1 2 M 1 3 S 0 2 5 0 2 I 0 69971 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 18707 3 0 2 S 1 1 M 12 36 S 0 1 5 0 2 I 0 50077 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 51533 3 0 2 S 1 2 M 28 84 5 0 2 I 0 13553 3 0 2 5 0 2 I 0 168828 3 0 2 M 13 39 S 0 1 5 0 2 I 0 28239 3 0 2 S 1 2 M 5 15 5 0 2 I 0 26930 3 0 2 M 6 18 S 0 2 5 0 2 I 0 5541 3 0 2 S 1 1 M 1 3 5 0 2 I 0 49709 3 0 2 M 6 18 5 0 2 I 0 65349 3 0 2 5 0 2 I 0 23336 3 0 2 M 13 39 5 0 2 I 0 39752 3 0 2 M 11 33 S 0 1 5 0 2 I 0 20189 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 77343 3 0 2 S 1 2 M 11 33 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 352062 1172129 124 + . gene_id 14 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 352062 352089 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 352062 352089 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 352090 352091 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 352090 378892 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 378891 378892 . + . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 378893 378931 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 378893 378931 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 378932 378933 . + . intron_id 2 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 378932 462629 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462636 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462636 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462637 462638 . + . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462637 532611 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 532610 532611 . + . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 532612 532629 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 532612 532629 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 532630 532631 . + . intron_id 4 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 532630 551340 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 551339 551340 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 551341 551378 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 551341 551378 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 551379 551380 . + . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 551379 601459 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 653014 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 653013 653014 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 653015 653100 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 653015 653100 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 653101 653102 . + . intron_id 7 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 653101 666657 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 835489 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 835488 835489 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 835490 835529 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 835490 835529 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 835530 835531 . + . intron_id 9 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 835530 863772 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 890723 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 890722 890723 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 890724 890743 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 890724 890743 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 890744 890745 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 890744 896288 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 896287 896288 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 896289 896292 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 896289 896292 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 896293 896294 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 896293 946005 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 946004 946005 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 946006 946023 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 946006 946023 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 946024 946025 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 946024 1011376 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1034716 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1034715 1034716 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1034717 1034755 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1034717 1034755 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1034756 1034757 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1034756 1074511 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1074510 1074511 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1074512 1074545 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1074512 1074545 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1074546 1074547 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1074546 1094738 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1094737 1094738 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1094739 1094747 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1094739 1094747 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1094748 1094749 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1094748 1172094 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1172093 1172094 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1172095 1172129 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1172095 1172129 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 352062 1172129 124 + . alignment_id 14 ; Query SPP00000007_1.0 ; Align 352062 4 27 ; Align 378895 14 36 ; Align 462632 27 3 ; Align 532613 29 15 ; Align 551342 35 36 ; Align 601462 48 15 ; Align 653017 54 84 ; Align 835490 82 39 ; Align 863775 96 15 ; Align 890724 101 18 ; Align 896290 108 3 ; Align 946006 109 18 ; Align 1034717 115 39 ; Align 1074512 128 33 ; Align 1094741 140 6 ; Align 1172097 143 33 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 123 Query range: 1 -> 194 Target range: 36498 -> 1167165 2 : SerLeu{G} >>>> Target Intron 1 >>>> {ly}ArgLeuCysArgLe : 9 ||||||{|} 11485 bp {||}! ! !|||!.!|| SerLeu{G}++ ++{ly}LeuThrCysHisLe 36499 : AGCTTA{G}gt.........................ag{GG}CTCACGTGTCACTT : 48005 10 : uLeuLysPro >>>> Target Intron 2 >>>> AlaLeuLeuCysGly{A : 18 |! !|||! ! 16729 bp |||:::|||||||||{ uTyrLysArg-+ -+AlaMetLeuCysGly{L 48006 : ATATAAGCGGct.........................tggctatgctgtgtggc{t : 64761 19 : l} >>>> Target Intron 3 >>>> {a}LeuAlaAlaPro >>>> Tar : 23 } 26115 bp {!}:!! !||| ! e}++ ++{u}IleHisAlaLys++ 64762 : t}gt.........................ag{G}ATTCATGCCAAGgt........ : 90893 24 : get Intron 4 >>>> GlyLeuAlaGlyThrMetCysAlaSerArg >>>> : 33 33888 bp |||:::...||| :::|||||| ++GlyValGlyGlyCysValCysAlaLeuGly++ 90894 : .................agggggtgggggggtgtgtatgtgcattgggggt..... : 124811 34 : Target Intron 5 >>>> Asp{A} >>>> Target Intron 6 >>>> : 34 46252 bp {|} 53663 bp ++Pro{A}++ + 124812 : ....................agCCA{G}gt.........................a : 224726 35 : {sp}TrpArgCysAlaArg >>>> Target Intron 7 >>>> SerMetH : 42 {||}||| !! ! !||| 173204 bp !!:!!| +{sp}TrpGlyLeuLeuArg++ ++ArgLeuH 224727 : g{AT}TGGGGGCTCCTTCGGgt.........................agCGGCTGC : 397955 43 : isGluPheSerAlaLysAspIleAsp{Gl} >>>> Target Intron 8 >>> : 51 || !! !!!!|||!!...!:!!! !{||} 13013 bp isProCysThrAlaAsnSerValGly{Gl}++ 397956 : ACCCATGTACAGCCAACAGTGTAGGC{GG}gt........................ : 397986 52 : > {y}HisMetVal{As} >>>> Target Intron 9 >>>> {n}LeuAs : 57 {|}||| :::{ } 109269 bp { } !.! ++{y}HisAlaIle{**}++ ++{*}LysGl 397987 : .ag{A}CATgcaata{tg}gt.........................ag{A}AAGCA : 520282 58 : pLysTyrArgGlyPheValCysIleValThrAsn{V} >>>> Target Intro : 69 .||| !|||||| ! !|||:!!! !! !|||{.} 190918 b nLysValArgGlyAlaSerCysValGluLysAsn{T}++ 520283 : GAAAGTTAGAGGTGCCAGCTGTGTTGAGAAAAAC{A}gt................. : 520321 70 : n 10 >>>> {al}AlaSerGlnUnkGlyLys >>>> Target Intron 11 : 76 p {.!}!.!!!!||| |||||| 46963 bp ++{hr}Gly***GlnGlyGlyLys++ 520322 : .........ag{CA}GGATGACAGGGAGGGAAGgt..................... : 711259 77 : >>>> ThrGluValAsnTyrThr{G} >>>> Target Intron 12 >>>> : 82 |||.!.:!!::::::|||{|} 64910 bp ++ThrHisLeuHisHisThr{G}++ 711260 : .....agACACATCTCCaccacaca{c}gt.......................... : 758241 83 : {ln}LeuValAspLeuHisAla{A} >>>> Target Intron 13 >>>> : 89 {||}|||! !! !|||||||||{ } 94969 bp ++{ln}LeuGlyGlyLeuHisAla{V}++ + 758242 : ag{AA}CTAGGAGGTCTCCATGCA{G}gt..........................a : 918137 90 : {rg}TyrAlaGluCysGlyLeuArgIleLeuAlaPheProCysAsnGlnPheGly : 106 { !}! !!.!||| !! !|||:!: ! ! ||| !|||! !!.! +{al}SerValGluThrThrTyrArgLeuGluAsnGluProProAsnProThrAla 918138 : g{TA}TCTGTGGAAACAACCTACCGACTGGAAAACGAGCCCCCCAACCCCACCGCA : 918189 107 : LysGlnGluProGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyrAsnVa : 125 !:!!...||||||:!! !......:::||||||::: ... ..! TrpLysSerProGlyAlaLeuHisArgLeuLysGluTyrProAsnSerLeuArgLy 918190 : TGGAAGAGCCCCGGGGCTCTCcatcgcctaaaagaataccctaattctCTGCGTAA : 918246 126 : lLysPheAspMetPheSerLysIleCysValAsnGlyAspAspAlaHisProLeu{ : 144 ! !.!.! !:!! :::|||::: ||| ||| ...|||:::{ sTrpIleValSerTyrIleValLeuCysLeuLeuGlyHisAspHisArgProIle{ 918247 : GTGGATAGTATCATACATtgtattatgcttattggggcatgaccacaggcctatt{ : 918303 145 : T} >>>> Target Intron 14 >>>> {rp}LysTrpMetLysIleGlnPr : 151 } 50447 bp {! }::::::::: ...|| G}++ ++{ly}ArgPheLeuThrThrAspPr 918304 : g}gt..........................ag{gc}agattccttaccactgatcC : 968771 152 : oLysGlyLys >>>> Target Intron 15 >>>> >>>> Target In : 155 | |||! ! 42594 bp 86371 oLeuGlyMet++ ++++ 968772 : ACTTGGCATGgt..........................aggt.............. : 1011379 156 : tron 16 >>>> GlyIleLeuGlyAsnAlaIleLysTrpAsn >>>> Targe : 165 bp ||| ::: ::: |||... 1 ++GlySerAsnLeuSerPheLeuHisTrpGln++ 1011380 : ............agggatcgaacctgagtttcctacattggcaggt.......... : 1097780 166 : t Intron 17 >>>> >>>> Target Intron 18 >>>> PheThrLy : 167 4370 bp 2161 bp |||||| ++++ +-PheThrTy 1097781 : ................aggt..........................aatttacgta : 1114315 168 : sPheLeuIleAsp{L} >>>> Target Intron 19 >>>> {ys}AsnGly : 174 ||| ::: { } 42559 bp { }...||| rPhe***LeuLys{A}++ ++{la}GlyGly 1114316 : tttttaattgaag{g}gt..........................ag{CT}GGGGGT : 1156895 175 : CysValValLysArgTyrGlyPro{Me} >>>> Target Intron 20 >>>> : 183 ||||||! !::: ! ! !!|||{ } 10208 bp CysValGlyArgSerGlnArgPro{Gl}+- 1156896 : TGTGTCGGCCGCTCTCAGCGCCCA{GG}gg.......................... : 1156926 184 : {t}GluGluProLeuValIleGluLysAspLeuPro : 194 { }.!....||||||:!!:!:..!:!!..!|||||| ++{y}AsnSerProLeuLeuLeuSerGluSerLeuPro 1156927 : ag{T}AATAGTCCCCTTCTGTTGTCAGAGTCCCTCCCT : 1167165 vulgar: SPP00000007_1.0 1 194 . NIVO01030317.1:subseq(555646,1182752) 36498 1167165 + 123 M 2 6 S 0 1 5 0 2 I 0 11481 3 0 2 S 1 2 M 8 24 5 0 2 I 0 16725 3 0 2 M 5 15 S 0 2 5 0 2 I 0 26111 3 0 2 S 1 1 M 4 12 5 0 2 I 0 33884 3 0 2 M 10 30 5 0 2 I 0 46248 3 0 2 M 1 3 S 0 1 5 0 2 I 0 53659 3 0 2 S 1 2 M 5 15 5 0 2 I 0 173200 3 0 2 M 11 33 S 0 2 5 0 2 I 0 13009 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 109265 3 0 2 S 1 1 M 13 39 S 0 1 5 0 2 I 0 190914 3 0 2 S 1 2 M 6 18 5 0 2 I 0 46959 3 0 2 M 6 18 S 0 1 5 0 2 I 0 64906 3 0 2 S 1 2 M 6 18 S 0 1 5 0 2 I 0 94965 3 0 2 S 1 2 M 54 162 S 0 1 5 0 2 I 0 50443 3 0 2 S 1 2 M 10 30 5 0 2 I 0 42590 3 0 2 5 0 2 I 0 86367 3 0 2 M 10 30 5 0 2 I 0 14366 3 0 2 5 0 2 I 0 2157 3 0 2 M 7 21 S 0 1 5 0 2 I 0 42555 3 0 2 S 1 2 M 10 30 S 0 2 5 0 2 I 0 10204 3 0 2 S 1 1 M 11 33 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 36499 1167165 123 + . gene_id 15 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 36499 36505 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 36499 36505 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 36506 36507 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 36506 47990 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 47989 47990 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 47991 48016 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 47991 48016 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 48017 48018 . + . intron_id 2 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 48017 64745 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64744 64745 . + . intron_id 1 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64746 64762 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64746 64762 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64763 64764 . + . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64763 90877 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 90876 90877 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 90878 90890 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 90878 90890 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 90891 90892 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 90891 124778 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 124777 124778 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 124779 124808 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 124779 124808 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 124809 124810 . + . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 124809 171060 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 224727 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 224726 224727 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 224728 224744 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 224728 224744 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 224745 224746 . + . intron_id 7 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 224745 397948 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 397947 397948 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 397949 397983 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 397949 397983 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 397984 397985 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 397984 410996 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 410995 410996 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 410997 411008 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 410997 411008 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 411009 411010 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 411009 520277 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 520276 520277 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 520278 520318 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 520278 520318 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 520319 520320 . + . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 520319 711236 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 711235 711236 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 711237 711256 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 711237 711256 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 711257 711258 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 711257 758219 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 758218 758219 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 758220 758238 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 758220 758238 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 758239 758240 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 758239 823148 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 823147 823148 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 823149 823169 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 823149 823169 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 823170 823171 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 823170 918138 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 918137 918138 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 918139 918303 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 918139 918303 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 918304 918305 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 918304 968750 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 968749 968750 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 968751 968782 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 968751 968782 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 968783 968784 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 968783 1011376 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1097747 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1097746 1097747 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1097748 1097777 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1097748 1097777 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1097778 1097779 . + . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1097778 1112147 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1114308 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1114307 1114308 . + . intron_id 17 ; splice_site "aa" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1114309 1114330 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1114309 1114330 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1114331 1114332 . + . intron_id 19 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1114331 1156889 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1156888 1156889 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1156890 1156923 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1156890 1156923 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1156924 1156925 . + . intron_id 20 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1156924 1167131 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1167130 1167131 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1167132 1167165 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1167132 1167165 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 36499 1167165 123 + . alignment_id 15 ; Query SPP00000007_1.0 ; Align 36499 2 6 ; Align 47993 5 24 ; Align 64746 13 15 ; Align 90879 19 12 ; Align 124779 23 30 ; Align 171061 33 3 ; Align 224730 35 15 ; Align 397949 40 33 ; Align 410998 52 9 ; Align 520279 56 39 ; Align 711239 70 18 ; Align 758220 76 18 ; Align 823151 83 18 ; Align 918141 90 162 ; Align 968753 145 30 ; Align 1097748 155 30 ; Align 1114309 165 21 ; Align 1156892 173 30 ; Align 1167133 184 33 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 121 Query range: 0 -> 195 Target range: 5695 -> 1154420 1 : MetSerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAla{L : 19 :!:|||||| !!!:!||| :::|||||| ||| ! !..! !!|||{ ValSerLeuArgLysLeuIleProIleLeuLysAlaAlaHisThrAlaArgAla{L 5696 : GTTAGCCTGAGAAAGCTGAtacccattctgaaggctgcacACACAGCCCGAGCA{A : 5750 20 : } >>>> Target Intron 1 >>>> {eu}AlaAlaProGlyLeuAlaGlyT : 27 } 33944 bp { !}|||!.! !..!! !||||||| }++ ++{ys}AlaGlyTyrAsnGlnAlaGlyT 5751 : }gt.........................ag{AA}GCTGGGTACAACCAGGCCGGAA : 39718 28 : hrMetCysAlaSerArgAsp >>>> Target Intron 2 >>>> AspTrpA : 36 || !..!|||||||||!!: 18303 bp ! !|||! hrGlyAlaAlaSerArgGlu+- +-GlyTrpL 39719 : CCGGGGCTGCCTCCAGAGAGgg.........................aaGGCTGGC : 58048 37 : rgCysAlaArg{Se} >>>> Target Intron 3 >>>> {r}MetHisGlu : 43 !||||||! !{||} 70782 bp {|}:!!|||! ! euCysAlaLeu{Se}++ ++{r}LeuHisAla 58049 : TGTGTGCTCTC{AG}gt.........................ag{C}CTGCATGCA : 128851 44 : PheSerAlaLysAspIle >>>> Target Intron 4 >>>> AspGlyHis : 52 .!! !!:!!||| ! 28537 bp !!:|||||| ProGlyProGlnAspGln++ ++GluGlyHis 128852 : CCAGGGCCCCAGGACCAAgt.........................agGAGGGCCAC : 157415 53 : Met >>>> Target Intron 5 >>>> ValAsnLeuAspLysTyrArgGly : 61 ..! 5815 bp :!!.!. !||| ! !:! ! Gln++ +-IleGluAsnAspHisAlaLysMet 157416 : CAGgt.........................acATAGAAAATGACCATGCAAAAATG : 163257 62 : PheValCysIleValThrAsnValAlaSerGlnUnkGlyLysThrGluValAsnTy : 80 ||| !|||::: :::... :::... ! ...|| PheProCysValHisLysHisAlaHisThrSerMetProLeuCysThrTyrThrTy 163258 : TTTCCATGTGtgcacaagcatgcacacacctcCATGCCACtttgtacatacacata : 163314 81 : r{Th} >>>> Target Intron 6 >>>> {r}Gln >>>> Target In : 83 |{||} 7742 bp {|}||| 96212 r{Th}++ ++{r}Gln++ 163315 : c{ac}gt.........................ag{C}CAGgt.............. : 171067 84 : tron 7 >>>> LeuValAspLeuHisAlaArgTyrAlaGluCys{G} >>>> : 94 bp ||| !! !! ! !.!!|||! ! ! !|||{|} ++LeuProAlaCysSerThrArgSerLeuLeuCys{G}++ 171068 : ...........agCTCCCCGCCTGTTCCACCCGATCCTTGCTGTGC{G}gt..... : 267313 95 : Target Intron 8 >>>> {ly}{L} >>>> Target Intron 9 >>>> : 95 5450 bp {||}{ } 46193 bp ++{ly}{G}++ 267314 : ....................ag{GA}{G}gt......................... : 272766 96 : {eu}ArgIleLeuAlaPhePro{C} >>>> Target Intron 10 >>>> : 102 { !}||| !! !|||!:!|||{ } 134098 bp ++{ly}ArgTyr***AlaTyrPro{G}++ + 272767 : ag{GC}CGCTATTAAGCTTATCCA{G}gt..........................a : 453074 103 : {ys}AsnGlnPheGlyLysGlnGluProGlySer >>>> Target Intron : 113 { }...:::||| :!!!:::!!||||||!!! 43060 bp +{lu}LysLysPheProGluArgLysProGlyArg++ 453075 : g{aa}aaaaaatttccgGAGCGCAAACCCGGGAGGgt................... : 453110 114 : 11 >>>> AsnGluGluIleLys{Gl} >>>> Target Intron 12 >>>> : 118 :::...:::|||{||} 45947 bp ++ProLysAsnValLys{Gl}++ 453111 : .......agccaaaaaatgttaaa{ga}gt.......................... : 496187 119 : {u}PheAlaAlaGlyTyrAsnValLysPheAspMet{Ph} >>>> Target : 130 {|}.!!:!!:!!|||! ... !||| ..! { } 245 ++{u}LeuSerSerGlySerGlyLysLysArgSerHis{Gl}++ 496188 : ag{A}CTCTCCTCCGGGTCAGGGAAGAAAAGGAGTCAT{GG}gt............ : 542170 131 : Intron 13 >>>> {e}SerLysIleCysValAsn{G} >>>> Target In : 137 38 bp { }!.!:::|||...!.!:::{|} 34734 ++{y}LysArgIleAlaAlaSer{G}++ 542171 : ..............ag{g}AAGCGCATTGCAGCTTCA{G}gt.............. : 566728 138 : tron 14 >>>> {ly}AspAspAlaHisPro{L} >>>> Target Intron : 143 bp {||}||| :::|||{ } 99477 bp ++{ly}AspLeuProAsnPro{A}++ 566729 : ............ag{gg}gatcttcccaatcca{g}gt.................. : 601480 144 : 15 >>>> {eu}TrpLysTrp{M} >>>> Target Intron 16 >>>> : 147 { !}|||!:! !{ } 162806 bp ++{sp}TrpArgMet{A}++ ++ 601481 : ........ag{AC}TGGAGGATG{G}gt..........................ag : 863771 148 : {et}LysIleGlnProLys >>>> Target Intron 17 >>>> >>>> : 153 { }||||||||| !:!! 147587 bp {sp}LysIleGlnPheGln++ ++++ 863772 : {AT}AAAATTCAATTCCAGgt..........................aggt..... : 1011379 154 : Target Intron 18 >>>> GlyLysGlyIleLeuGlyAsnAlaIleLysTrp : 163 48105 bp |||:!!|||:!!||||||...|||! ! !!:: -+GlyGlnGlyLeuLeuGlyGlyAlaArgValPhe 1011380 : .....................ggGGACAAGGTCTCCTCGGGGGAGCGAGAGTGTTC : 1059512 164 : AsnPheThrLysPheLeuIleAspLysAsn{G} >>>> Target Intron 19 : 174 ....!.! !:!! :!!:!!...:!!:!!{ } 53212 bp ArgMetIleGluAlaValValSerGlnHis{*}++ 1059513 : CGGATGATAGAAGCGGTGGTCTCACAGCAC{T}gt..................... : 1059548 175 : >>>> {ly}CysValValLysArgTyrGlyProMetGluGluProLeuValIle : 189 { !} ::: !:!||||||.!. .!. !!!!|||:!: ++{**}MetSerIle***ThrPheGlyProPheLeuAsnCysPheValVal 1059549 : .....ag{aa}atgtctatttagacgTTTGGCCCATTTCTCAATTGTTTTGTTGTG : 1112802 190 : GluLys{A} >>>> Target Intron 20 >>>> {sp}LeuProHis : 195 ||| !{.} 41598 bp {..} !|||||| GluLeu{S}++ ++{er}SerProHis 1112803 : GAGTTG{T}gt..........................ag{ca}tcCCCCCAC : 1154420 vulgar: SPP00000007_1.0 0 195 . NIVO01030317.1:subseq(555646,1182752) 5695 1154420 + 121 M 18 54 S 0 1 5 0 2 I 0 33940 3 0 2 S 1 2 M 14 42 5 0 2 I 0 18299 3 0 2 M 6 18 S 0 2 5 0 2 I 0 70778 3 0 2 S 1 1 M 9 27 5 0 2 I 0 28533 3 0 2 M 4 12 5 0 2 I 0 5811 3 0 2 M 27 81 S 0 2 5 0 2 I 0 7738 3 0 2 S 1 1 M 1 3 5 0 2 I 0 96208 3 0 2 M 11 33 S 0 1 5 0 2 I 0 5446 3 0 2 S 1 3 5 0 2 I 0 46189 3 0 2 S 1 2 M 6 18 S 0 1 5 0 2 I 0 134094 3 0 2 S 1 2 M 10 30 5 0 2 I 0 43056 3 0 2 M 5 15 S 0 2 5 0 2 I 0 45943 3 0 2 S 1 1 M 11 33 S 0 2 5 0 2 I 0 24534 3 0 2 S 1 1 M 6 18 S 0 1 5 0 2 I 0 34730 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 99473 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 162802 3 0 2 S 1 2 M 5 15 5 0 2 I 0 147583 3 0 2 5 0 2 I 0 48101 3 0 2 M 21 63 S 0 1 5 0 2 I 0 53208 3 0 2 S 1 2 M 17 51 S 0 1 5 0 2 I 0 41594 3 0 2 S 1 2 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 5696 1154420 121 + . gene_id 16 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 5696 5750 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 5696 5750 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 5751 5752 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 5751 39694 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 39693 39694 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 39695 39738 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 39695 39738 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 39739 39740 . + . intron_id 2 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 39739 58041 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 58040 58041 . + . intron_id 1 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 58042 58061 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 58042 58061 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 58062 58063 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 58062 128843 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 128842 128843 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 128844 128871 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 128844 128871 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 128872 128873 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 128872 157408 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 157407 157408 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 157409 157420 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 157409 157420 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 157421 157422 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 157421 163235 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 163234 163235 . + . intron_id 4 ; splice_site "AC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 163236 163318 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 163236 163318 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 163319 163320 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 163319 171060 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 267276 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 267275 267276 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 267277 267310 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 267277 267310 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 267311 267312 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 267311 272760 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 272759 272760 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 272761 272763 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 272761 272763 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 272764 272765 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 272764 318956 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 318955 318956 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 318957 318977 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 318957 318977 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 318978 318979 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 318978 453075 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 453074 453075 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 453076 453107 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 453076 453107 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 453108 453109 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 453108 496167 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 496166 496167 . + . intron_id 10 ; splice_site "Ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 496168 496184 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 496168 496184 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 496185 496186 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 496185 542131 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 542130 542131 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 542132 542167 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 542132 542167 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 542168 542169 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 542168 566705 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 566704 566705 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 566706 566725 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 566706 566725 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 566726 566727 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 566726 601459 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 700954 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 700953 700954 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 700955 700966 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 700955 700966 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 700967 700968 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 700967 863772 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 1011376 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1059481 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1059480 1059481 . + . intron_id 17 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1059482 1059545 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1059482 1059545 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1059546 1059547 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1059546 1112757 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112756 1112757 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1112758 1112811 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1112758 1112811 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112812 1112813 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112812 1154409 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1154408 1154409 . + . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1154410 1154420 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1154410 1154420 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 5696 1154420 121 + . alignment_id 16 ; Query SPP00000007_1.0 ; Align 5696 1 54 ; Align 39697 20 42 ; Align 58042 34 18 ; Align 128845 41 27 ; Align 157409 50 12 ; Align 163236 54 81 ; Align 171062 82 3 ; Align 267277 83 33 ; Align 318959 96 18 ; Align 453078 103 30 ; Align 496168 113 15 ; Align 542133 119 33 ; Align 566707 131 18 ; Align 601462 138 15 ; Align 700957 144 9 ; Align 863775 148 15 ; Align 1059482 153 63 ; Align 1112760 175 51 ; Align 1154412 193 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 118 Query range: 1 -> 163 Target range: 134568 -> 1070061 2 : SerLeuGlyArg >>>> Target Intron 1 >>>> LeuCysArgLeuLeu : 10 |||||||||!:! 132696 bp ||| ! !! !! ! SerLeuGlyGln++ ++LeuProAlaCysSer 134569 : AGCCTGGGCCAGgt.........................agCTCCCCGCCTGTTCC : 267289 11 : LysProAlaLeuLeuCys{G} >>>> Target Intron 2 >>>> {ly}{A : 18 ! ! !:!!|||||||||{|} 5450 bp {||}{| ThrArgSerLeuLeuCys{G}++ ++{ly}{A 267290 : ACCCGATCCTTGCTGTGC{G}gt.........................ag{GA}{G : 272763 19 : } >>>> Target Intron 3 >>>> {la}LeuAlaAlaProGlyLeuAlaG : 26 } 14909 bp {||}! !!.! !! !!.!|||.!!| }++ ++{la}GlnValTrpGlnAlaLeuThrG 272764 : }gt.........................ag{CC}CAAGTGTGGCAGGCACTGACTG : 287696 27 : lyThrMetCysAlaSerArgAspAspTrpArgCysAlaArgSerMetHisGluPhe : 44 || !:!: !.!||| !! !||| !||||||!.! !!!!! !|||! ! ! lyGlyLeuLeuGlySerGlyProAspLeuArgCysValAspThrArgHisGlyGly 287697 : GTGGGCTTCTGGGTTCTGGACCCGACCTGAGGTGTGTAGACACCAGGCACGGAGGC : 287750 45 : SerAlaLys{A} >>>> Target Intron 4 >>>> {sp}IleAspGlyHi : 52 |||!.!!!.{!} 24268 bp {!:} :::|||.. SerGlyAsn{G}++ ++{lu}ArgAsnGlyAr 287751 : TCTGGGAAC{G}gt.........................ag{ag}agaaatGGGAG : 312042 53 : sMetVal{As} >>>> Target Intron 5 >>>> {n}LeuAspLysTyrA : 60 .:!: !{!.} 26720 bp {.} !||||||:!!! gValCys{Ar}++ ++{g}SerAspLysHisG 312043 : GGTCTGC{AG}gt.........................ag{G}AGTGATAAACACC : 338786 61 : rg >>>> Target Intron 6 >>>> GlyPheValCysIleValThrAsnV : 69 :! 111008 bp ||||||:::|||::: ...:::| ln++ -+GlyPheLeuCysValCysValSerV 338787 : AGgt.........................tgggtttcctctgtgtgtgtgtgtctg : 449821 70 : al{A} >>>> Target Intron 7 >>>> {la}SerGlnUnkGlyLysThr : 76 ||{ } 32485 bp { !}:!! |||!:!:!! al{T}++ ++{yr}AlaThrProGlyArgSer 449822 : tc{t}gt.........................ag{AT}GCCACTCCTGGAAGATCC : 482327 77 : Glu{V} >>>> Target Intron 8 >>>> {al}AsnTyrThr{G} >>> : 82 :!!{!} 90634 bp { !}|||||||||{.} Gln{G}++ -+{ly}AsnTyrThr{A}++ 482328 : CAA{G}gt.........................tg{GA}AACTACACA{G}gt... : 572982 83 : > Target Intron 9 >>>> {ln}LeuValAspLeuHisAlaArgTyrAlaG : 92 78445 bp {!.} !! ! !! !.!.|||||| ! !: ++{sp}ThrGluHisThrGluAlaArgThrPheL 572983 : ......................ag{AC}ACAGAACATACAGAAGCAAGAACCTTTA : 651454 93 : luCysGlyLeuArgIle{L} >>>> Target Intron 10 >>>> {eu}Al : 99 !! !|||||||||:!!{!} 48001 bp { !}:: ysThrGlyLeuArgLeu{T}++ ++{rp}Se 651455 : AAACTGGCTTACGGCTA{T}gt..........................ag{gg}tc : 699476 100 : aPheProCysAsnGlnPheGlyLysGln{G} >>>> Target Intron 11 > : 109 :|||||| ||||||||| ...{|} 163203 bp rPheProMetAsnGlnPhePheThrSer{G}++ 699477 : ttttccaatgaatcagttcttcacatca{g}gt....................... : 699509 110 : >>> {lu}ProGlySerAsnGluGluIleLysGluPhe{A} >>>> Target : 120 {||} !|||:!!.....!:!!!!:!...!.|||{|} 138 ++{lu}ArgGlyAlaGlyArgGlnMetSerAsnPhe{A}++ 699510 : ...ag{AG}AGAGGAGCTGGAAGGCAGATGAGCAATTTC{G}gt............ : 862745 121 : Intron 12 >>>> {la}AlaGlyTyrAsnValLysPheAspMetPheSerLys : 132 582 bp {||}::: :::::::::||| !!! .!.|||:!! ++{la}SerPhePheSerLeuLysSerHisArgLeuSerGlu 862746 : ..............ag{ct}tcgttcttttccttaaaatctcACAGACTGTCTGAG : 1001360 133 : IleCysValAsn{G} >>>> Target Intron 13 >>>> {ly}AspAspA : 140 !! !||||||{!} 8623 bp {.!}...:::: CysSerValAsn{A}-+ ++{la}SerGluS 1001361 : TGCTCTGTAAAT{G}ct..........................ag{ca}tcagagt : 1010007 141 : laHisPro >>>> Target Intron 14 >>>> >>>> Target Intr : 143 :: ||| 1361 bp 30737 b erPhePro++ ++++ 1010008 : cttttccagt..........................aggt................ : 1011379 144 : on 15 >>>> LeuTrpLysTrpMetLys{Il} >>>> Target Intron 1 : 149 p :!!||| ! ! ...{ } 27885 bp ++IleTrpValGlyLysSer{Gl}++ 1011380 : ..........agaTTTGGGTTGGTAAATCT{GG}gt.................... : 1042136 150 : 6 >>>> {e}GlnProLysGlyLysGlyIleLeuGlyAsnAlaIleLysTrp : 163 {!}:::|||:::|||:::||| ||||||..!..! ! !||| ++{y}GluProGlnGlyArgGlySerLeuGlyArgCysArgLeuTrp 1042137 : ......ag{a}gagccccagggacgggggagcctggggcgCTGCCGCCTGTGG : 1070061 vulgar: SPP00000007_1.0 1 163 . NIVO01030317.1:subseq(555646,1182752) 134568 1070061 + 118 M 4 12 5 0 2 I 0 132692 3 0 2 M 11 33 S 0 1 5 0 2 I 0 5446 3 0 2 S 1 3 5 0 2 I 0 14905 3 0 2 S 1 2 M 29 87 S 0 1 5 0 2 I 0 24264 3 0 2 S 1 2 M 6 18 S 0 2 5 0 2 I 0 26716 3 0 2 S 1 1 M 5 15 5 0 2 I 0 111004 3 0 2 M 9 27 S 0 1 5 0 2 I 0 32481 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 90630 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 78441 3 0 2 S 1 2 M 15 45 S 0 1 5 0 2 I 0 47997 3 0 2 S 1 2 M 10 30 S 0 1 5 0 2 I 0 163199 3 0 2 S 1 2 M 10 30 S 0 1 5 0 2 I 0 138578 3 0 2 S 1 2 M 16 48 S 0 1 5 0 2 I 0 8619 3 0 2 S 1 2 M 5 15 5 0 2 I 0 1357 3 0 2 5 0 2 I 0 30733 3 0 2 M 6 18 S 0 2 5 0 2 I 0 27881 3 0 2 S 1 1 M 14 42 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 134569 1070061 118 + . gene_id 17 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 134569 134580 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 134569 134580 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 134581 134582 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 134581 267276 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 267275 267276 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 267277 267310 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 267277 267310 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 267311 267312 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 267311 272760 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 272759 272760 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 272761 272763 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 272761 272763 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 272764 272765 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 272764 287672 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 287671 287672 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 287673 287762 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 287673 287762 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 287763 287764 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 287763 312030 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 312029 312030 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 312031 312052 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 312031 312052 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 312053 312054 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 312053 338772 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 338771 338772 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 338773 338788 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 338773 338788 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 338789 338790 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 338789 449796 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 449795 449796 . + . intron_id 5 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 449797 449824 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 449797 449824 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 449825 449826 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 449825 482309 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 482308 482309 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 482310 482333 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 482310 482333 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 482334 482335 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 482334 572967 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 572966 572967 . + . intron_id 7 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 572968 572979 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 572968 572979 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 572980 572981 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 572980 651424 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 651423 651424 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 651425 651472 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 651425 651472 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 651473 651474 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 651473 699473 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 699472 699473 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 699474 699506 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 699474 699506 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 699507 699508 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 699507 862709 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 862708 862709 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 862710 862742 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 862710 862742 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 862743 862744 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 862743 1001324 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1001323 1001324 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1001325 1001375 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1001325 1001375 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1001376 1001377 . + . intron_id 13 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1001376 1009998 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1009997 1009998 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1009999 1010015 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1009999 1010015 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1010016 1010017 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1010016 1011376 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1042113 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1042112 1042113 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1042114 1042133 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1042114 1042133 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1042134 1042135 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1042134 1070018 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1070017 1070018 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1070019 1070061 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1070019 1070061 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 134569 1070061 118 + . alignment_id 17 ; Query SPP00000007_1.0 ; Align 134569 2 12 ; Align 267277 6 33 ; Align 287675 19 87 ; Align 312033 49 18 ; Align 338774 56 15 ; Align 449797 61 27 ; Align 482312 71 21 ; Align 572970 79 9 ; Align 651427 83 45 ; Align 699476 99 30 ; Align 862712 110 30 ; Align 1001327 121 48 ; Align 1010001 138 15 ; Align 1042114 143 18 ; Align 1070020 150 42 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 118 Query range: 16 -> 196 Target range: 54120 -> 1148054 17 : GlyAlaLeuAlaAlaProGlyLeuAlaGlyThrMetCys{A} >>>> Target : 30 |||!.! !||| !||||||||| !!||| !:!:|||{.} 240 GlyValAlaAlaPheProGlyLeuProGlyLeuLeuCys{C}++ 54121 : GGTGTAGCTGCTTTCCCAGGGCTCCCTGGCCTCCTCTGC{T}gt............ : 54163 31 : Intron 1 >>>> {la}SerArgAspAspTrpArgCysAlaArgSerMet >> : 42 2 bp {.!}|||||||||.!.|||||| ! !! ! !!:!: ++{ys}SerArgAspGlnTrpArgAspProAlaProLeu++ 54164 : .............ag{GT}TCACGTGATCAATGGAGAGACCCTGCTCCGCTCgt.. : 56600 43 : >> Target Intron 2 >>>> HisGluPheSerAlaLysAspIleAspGlyH : 52 41552 bp ||| ...::: ::: | ++GlyIlePheProThrGlnGlySerAsnProH 56601 : .......................aggggatattcccaacccagggatcgaacccac : 98180 53 : isMetValAsnLeuAspLysTyrArg{G} >>>> Target Intron 3 >>>> : 61 ||:::::::::||| {|} 48173 bp isLeuLeuHisLeuLeuAlaLeuAla{G}++ 98181 : atctcttacatctacttgcattggca{g}gt......................... : 98210 62 : {ly}PheValCysIleValThrAsnValAlaSerGlnUnkGlyLysThrGluVa : 78 {||} ! !..!:!!:!! !!...|||..!! !||| |||! !:!... ++{ly}ArgAspAlaLeuIleProGlyValCysLeuGlnProGlyThrSerSerGl 98211 : ag{GA}AGGGATGCCCTCATACCTGGGGTGTGCTTGCAACCTGGGACCAGCAGCCA : 146431 79 : l >>>> Target Intron 4 >>>> AsnTyrThrGlnLeuValAspLeuHi : 87 ! 57030 bp .!. ||| |||||| !! !!. n++ -+GluValThrValLeuValThrProAr 146432 : Ggt.........................cgGAGGTAACAGTCCTGGTAACCCCCCG : 203488 88 : sAlaArgTyr >>>> Target Intron 5 >>>> AlaGluCysGlyLeuAr : 96 . !||| ! 90033 bp |||! !||||||||||| gLeuArgGln++ +-AlaValCysGlyLeuAr 203489 : ACTCCGTCAGgt.........................atGCGGTGTGCGGGCTcag : 293548 97 : gIle{L} >>>> Target Intron 6 >>>> {eu}AlaPheProCysAsnG : 104 |:::{!} 96174 bp { !}:::|||||| :::| gLeu{T}++ ++{rp}SerPheProMetSerG 293549 : gcta{t}gt.........................ag{gg}tctttcccaatgagtc : 389746 105 : lnPheGlyLysGln{G} >>>> Target Intron 7 >>>> {lu}ProGly : 111 ||||| ...{!} 37823 bp {!:} ! ! lnPhePheAlaSer{A}++ ++{sp}GlyGln 389747 : agttcttcgcttca{g}gt.........................ag{AC}GGTCAG : 427590 112 : SerAsnGluGluIleLysGluPheAlaAlaGlyTyrAsnValLysPheAspMetPh : 130 !:!! |||:!:...|||||| !! !! ! !!..|||||||||... !.! LeuAspThrGluValSerGluPheTyrGluGluThrThrValLysPheSerGluLe 427591 : CTGGACACTGAGGTGTCCGAGTTCTACGAGGAGACCACGGTGAAGTTCTCGGAGCT : 427647 131 : eSer >>>> Target Intron 8 >>>> LysIleCysValAsn >>>> T : 137 .||| 34977 bp ::: |||:::::: uSer+- ++GluSerCysMetAsp++ 427648 : GTCGga.........................aggagtcatgtatggatgt...... : 462647 138 : arget Intron 9 >>>> GlyAspAspAlaHisProLeuTrpLysTrpMetLy : 148 21257 bp |||!!:||| !.!.||| !||| ! :!! -+GlyGluAspGlnGluProThrTrpLeuAlaLeuAl 462648 : ...................cgGGCGAGGACCAGGAGCCCACGTGGCTGGCCCTGGC : 483935 149 : sIleGlnProLys{Gl} >>>> Target Intron 10 >>>> {y}LysGly : 155 ! ! |||!..{ !} 82754 bp {!}||| !! aThrValProSer{Tr}++ ++{p}LysArg 483936 : CACCGTCCCGAGC{TG}gt..........................ag{g}AAGCGC : 566710 156 : IleLeuGlyAsn{A} >>>> Target Intron 11 >>>> {la}IleLysT : 163 ||| !!.!:::{|} 44619 bp {||}||| | IleAlaAlaSer{A}++ ++{la}IleProT 566711 : ATTGCAGCTTCA{G}gt..........................ag{CC}ATACCCT : 611353 164 : rpAsnPheThrLysPheLeuIle{As} >>>> Target Intron 12 >>>> : 171 || .!.|||!:! !:!!! !{! } 33183 bp rpLeuIleThrArgGlyIleThr{Gl}++ + 611354 : GGTTAATAACCAGGGGTATTACC{GG}gt..........................a : 644560 172 : {p}LysAsnGlyCysValValLysArg{T} >>>> Target Intron 13 > : 180 { }:!!!!.|||||| !!.!..!! !{ } 15503 bp +{y}GlnLysGlyCysSerAlaSerPro{G}+- 644561 : g{G}CAGAAAGGATGCTCAGCTTCGCCA{G}ga....................... : 644590 181 : >>> {yr}GlyProMetGluGlu >>>> Target Intron 14 >>>> : 186 {! }...||| |||::: 6550 bp ++{lu}AsnProLysGluAsp++ ++++ 644591 : ...ag{aa}aaccccaaagaggatgt..........................aggt : 666658 187 : >>>> Target Intron 15 >>>> ProLeuValIleGluLysAsp >>>> : 193 153812 bp ||| !! ! !||||||!!: ++ProArgGlyTyrGluLysGlu++ 666659 : ..........................agCCAAGAGGCTACGAGAAGGAGgt..... : 820493 194 : Target Intron 16 >>>> >>>> Target Intron 17 >>>> > : 193 190886 bp 100771 bp ++++ ++++ 820494 : .....................aggt..........................aggt. : 1112150 194 : >>> Target Intron 18 >>>> LeuProHisTyr : 196 35895 bp !||||||!:! ++ArgProHisPhe 1112151 : .........................agAGACCCCATTTC : 1148054 vulgar: SPP00000007_1.0 16 196 . NIVO01030317.1:subseq(555646,1182752) 54120 1148054 + 118 M 13 39 S 0 1 5 0 2 I 0 2398 3 0 2 S 1 2 M 11 33 5 0 2 I 0 41548 3 0 2 M 19 57 S 0 1 5 0 2 I 0 48169 3 0 2 S 1 2 M 17 51 5 0 2 I 0 57026 3 0 2 M 12 36 5 0 2 I 0 90029 3 0 2 M 7 21 S 0 1 5 0 2 I 0 96170 3 0 2 S 1 2 M 10 30 S 0 1 5 0 2 I 0 37819 3 0 2 S 1 2 M 22 66 5 0 2 I 0 34973 3 0 2 M 5 15 5 0 2 I 0 21253 3 0 2 M 16 48 S 0 2 5 0 2 I 0 82750 3 0 2 S 1 1 M 6 18 S 0 1 5 0 2 I 0 44615 3 0 2 S 1 2 M 10 30 S 0 2 5 0 2 I 0 33179 3 0 2 S 1 1 M 8 24 S 0 1 5 0 2 I 0 15499 3 0 2 S 1 2 M 5 15 5 0 2 I 0 6546 3 0 2 5 0 2 I 0 153808 3 0 2 M 7 21 5 0 2 I 0 190882 3 0 2 5 0 2 I 0 100767 3 0 2 5 0 2 I 0 35891 3 0 2 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 54121 1148054 118 + . gene_id 18 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 54121 54160 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 54121 54160 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 54161 54162 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 54161 56562 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 56561 56562 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 56563 56597 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 56563 56597 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 56598 56599 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 56598 98149 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 98148 98149 . + . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 98150 98207 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 98150 98207 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 98208 98209 . + . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 98208 146380 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146379 146380 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146381 146433 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146381 146433 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146434 146435 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146434 203463 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 203462 203463 . + . intron_id 3 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 203464 203499 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 203464 203499 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 203500 203501 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 203500 293532 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 293531 293532 . + . intron_id 4 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 293533 293554 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 293533 293554 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 293555 293556 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 293555 389728 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 389727 389728 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 389729 389761 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 389729 389761 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 389762 389763 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 389762 427584 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 427583 427584 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 427585 427652 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 427585 427652 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 427653 427654 . + . intron_id 8 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 427653 462629 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462644 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462644 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462645 462646 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462645 483901 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 483900 483901 . + . intron_id 8 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 483902 483951 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 483902 483951 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 483952 483953 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 483952 566705 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 566704 566705 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 566706 566725 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 566706 566725 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 566726 566727 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 566726 611344 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 611343 611344 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 611345 611378 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 611345 611378 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 611379 611380 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 611379 644561 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 644560 644561 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 644562 644587 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 644562 644587 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 644588 644589 . + . intron_id 13 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 644588 660090 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 660089 660090 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 660091 660107 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 660091 660107 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 660108 660109 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 660108 666657 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 820469 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 820468 820469 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 820470 820490 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 820470 820490 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 820491 820492 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 820491 1011376 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1112147 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1148042 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1148041 1148042 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1148043 1148054 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1148043 1148054 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 54121 1148054 118 + . alignment_id 18 ; Query SPP00000007_1.0 ; Align 54121 17 39 ; Align 56565 31 33 ; Align 98150 42 57 ; Align 146383 62 51 ; Align 203464 79 36 ; Align 293533 91 21 ; Align 389731 99 30 ; Align 427587 110 66 ; Align 462630 132 15 ; Align 483902 137 48 ; Align 566707 154 18 ; Align 611347 161 30 ; Align 644563 172 24 ; Align 660093 181 15 ; Align 820470 186 21 ; Align 1148043 193 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 118 Query range: 1 -> 162 Target range: 160601 -> 1178778 2 : SerLeuGly{Ar} >>>> Target Intron 1 >>>> {g}LeuCysArgLe : 9 |||||||||{||} 96803 bp {|}:!!||| !! SerLeuGly{Ar}++ -+{g}MetCysTyrCy 160602 : TCCCTGGGG{AG}gt.........................tg{G}ATGTGCTACTG : 257426 10 : uLeu{Ly} >>>> Target Intron 2 >>>> {s}ProAlaLeu{L} >> : 15 !|||{!:} 6203 bp {!}||||||! !{ } sLeu{Ar}++ ++{g}ProAlaSer{A}++ 257427 : CCTG{AG}gt.........................ag{A}CCTGCTTCT{G}gt.. : 263650 16 : >> Target Intron 3 >>>> {eu}CysGlyAlaLeuAlaAlaPro{G} > : 23 151926 bp { !} |||||| !|||||||||{|} ++{la}LeuGlyAlaGlyAlaAlaPro{G}++ 263651 : .......................ag{CA}CTGGGGGCGGGGGCTGCCCCt{g}gt. : 415600 24 : >>> Target Intron 4 >>>> {ly}LeuAla{G} >>>> Target Int : 26 7753 bp {||}|||!.!{|} 39270 ++{ly}LeuGly{G}++ 415601 : ........................ag{GG}CTGGGA{G}gt............... : 423362 27 : ron 5 >>>> {ly}Thr{Me} >>>> Target Intron 6 >>>> {t}C : 29 bp {||}...{||} 39028 bp {|} ++{ly}Val{Me}++ ++{t}G 423363 : ..........ag{ga}gtc{at}gt.........................ag{g}g : 501666 30 : ysAlaSer{Ar} >>>> Target Intron 7 >>>> {g}Asp{A} >>>> : 34 |||{||} 86850 bp {|}! {|} luPheSer{Ar}++ ++{g}Ala{A}++ 501667 : aattttcc{ag}gt.........................ag{G}GCA{G}gt.... : 588534 35 : Target Intron 8 >>>> {sp}TrpArgCys{A} >>>> Target Int : 38 112423 bp {||}|||||| {|} 89659 ++{sp}TrpArgMet{A}++ 588535 : .....................ag{AC}TGGAGGATG{G}gt............... : 700969 39 : ron 9 >>>> {la}Arg{Se} >>>> Target Intron 10 >>>> {r} : 40 bp {||}|||{!!} 39330 bp {!} ++{la}Arg{Ar}++ ++{g} 700970 : ..........ag{CC}AGA{AG}gt..........................ag{A} : 829963 41 : MetHisGluPheSerAlaLysAspIleAspGlyHis{M} >>>> Target Int : 53 !!::!! .!!!!!||| |||:!: ! !!!!.{ } 33504 IleTyrThrLeuCysAlaProAspLeuProArgGln{A}++ 829964 : ATCTACACTCTCTGTGCTCCTGACTTGCCTAGGCAA{G}gt............... : 830003 54 : ron 11 >>>> {et}ValAsnLeuAspLysTyrArgGlyPheVal{C} >>>> : 64 bp { } ||||||...:::::::::|||||| { } -+{la}PheAsnLeuSerGlnHisGlnGlyPheSer{S}++ 830004 : ...........cg{cc}ttcaatctttcccagcatcagggtttttcc{a}gt.... : 863540 65 : Target Intron 12 >>>> {ys}IleValThrAsnValAlaSerGlnUnkG : 74 35860 bp {!!} !:!! ! ! !.!!!!!:!! . ++{er}AlaIleGln******ThrThrGluArgA 863541 : ......................ag{GT}GCTATTCAGTAATAGACTACTGAAAGAA : 899427 75 : lyLysThrGluValAsnTyr{Th} >>>> Target Intron 13 >>>> {r : 81 .!!....!|||:!!||||||{! } 60541 bp {! snSerValGluLeuAsnTyr{Ly}++ ++{s 899428 : ATAGTGTGGAATTAAATtac{aa}gt..........................ag{g : 959991 82 : }GlnLeuValAspLeuHisAlaArgTyrAla{G} >>>> Target Intron 1 : 92 }...|||::: ||||||...:::|||:::{.} 32403 bp }SerLeuLeuValLeuHisValLysTyrSer{A}++ 959992 : }tccttgttagttctccatgttaaatacagc{a}gt.................... : 960025 93 : 4 >>>> {lu}CysGlyLeuArgIleLeuAlaPheProCysAsnGln{Ph} >> : 105 {.!} ||| ... !!.!! ||||||! { } ++{rg}IleLysLeuThrPheAlaValSerProCysIleAla{Ar}++ 960026 : ......ag{gg}atcaaactcacatttgCTGTGTCACCTTGCATtgcc{ag}gt.. : 992468 106 : >> Target Intron 15 >>>> {e}GlyLysGlnGluProGlySerAsnGlu : 114 72714 bp { }|||...... ||||||...... ++{g}GlySerSerProProGlyAspArgThr 992469 : ........................ag{g}ggatcttccccgcccggggatcgaacc : 1065205 115 : GluIleLysGluPheAlaAlaGlyTyrAsnVal{Ly} >>>> Target Intro : 126 ::::::......... ...|||::::::...{!.} 31907 bp GlnValSerArgIleArgGlyGlyPheSerThr{Se}-+ 1065206 : caggtgtcccgcattagaggcggattctctacc{ag}ct................. : 1065245 127 : n 16 >>>> {s}PheAspMetPheSer{L} >>>> Target Intron 17 : 132 {.}... :::||||||{!} 19480 bp ++{r}IleArgValPheSer{A}++ 1065246 : .........ag{c}atcagggtcttttcc{a}gt...................... : 1097169 133 : >>>> {ys}IleCysValAsn >>>> Target Intron 18 >>>> GlyA : 138 {!.}|||||| ... 62040 bp ! ++{sn}IleCysSerGly++ ++ThrP 1097170 : ....ag{at}atatgctcaggagt..........................agACTC : 1178704 139 : spAspAlaHisProLeuTrpLysTrpMetLysIleGlnProLysGlyLysGlyIle : 156 !|||..!.!.|||||| ! ! ! ! ||| ! |||:!!|||!!: roAspCysGluProLeuLeuTyrIleSerAlaTyrAlaProHisGlyGlnGlyMet 1178705 : CTGACTGTGAGCCCTTGCTGTATATAAGCGCCTATGCTCCTCATGGGCAGGGGATG : 1178758 157 : LeuGlyAsnAlaIleLys : 162 !|||!..!.!:!!:!! GlyGlyThrValLeuGlu 1178759 : GGGGGCACAGTCCTTGAG : 1178778 vulgar: SPP00000007_1.0 1 162 . NIVO01030317.1:subseq(555646,1182752) 160601 1178778 + 118 M 3 9 S 0 2 5 0 2 I 0 96799 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 6199 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 151922 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 7749 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 39266 3 0 2 S 1 2 M 1 3 S 0 2 5 0 2 I 0 39024 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 86846 3 0 2 S 1 1 M 1 3 S 0 1 5 0 2 I 0 112419 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 89655 3 0 2 S 1 2 M 1 3 S 0 2 5 0 2 I 0 39326 3 0 2 S 1 1 M 12 36 S 0 1 5 0 2 I 0 33500 3 0 2 S 1 2 M 10 30 S 0 1 5 0 2 I 0 35856 3 0 2 S 1 2 M 16 48 S 0 2 5 0 2 I 0 60537 3 0 2 S 1 1 M 10 30 S 0 1 5 0 2 I 0 32399 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 72710 3 0 2 S 1 1 M 20 60 S 0 2 5 0 2 I 0 31903 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 19476 3 0 2 S 1 2 M 4 12 5 0 2 I 0 62036 3 0 2 M 26 78 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 160602 1178778 118 + . gene_id 19 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 160602 160612 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 160602 160612 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 160613 160614 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 160613 257415 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 257414 257415 . + . intron_id 0 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 257416 257433 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 257416 257433 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 257434 257435 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 257434 263636 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 263635 263636 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 263637 263647 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 263637 263647 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 263648 263649 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 263648 415573 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 415572 415573 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 415574 415597 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 415574 415597 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 415598 415599 . + . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 415598 423350 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 423349 423350 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 423351 423359 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 423351 423359 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 423360 423361 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 423360 462629 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462636 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462636 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462637 462638 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462637 501664 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 501663 501664 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 501665 501676 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 501665 501676 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 501677 501678 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 501677 588526 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 588525 588526 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 588527 588531 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 588527 588531 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 588532 588533 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 588532 700954 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 700953 700954 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 700955 700966 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 700955 700966 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 700967 700968 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 700967 790625 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 790624 790625 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 790626 790632 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 790626 790632 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 790633 790634 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 790633 829962 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 829961 829962 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 829963 830000 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 829963 830000 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 830001 830002 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 830001 863504 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863503 863504 . + . intron_id 10 ; splice_site "cg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863505 863537 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863505 863537 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863538 863539 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863538 899397 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 899396 899397 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 899398 899449 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 899398 899449 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 899450 899451 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 899450 959990 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 959989 959990 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 959991 960022 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 959991 960022 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 960023 960024 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 960023 992425 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 992424 992425 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 992426 992465 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 992426 992465 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 992466 992467 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 992466 1065179 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1065178 1065179 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1065180 1065242 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1065180 1065242 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1065243 1065244 . + . intron_id 16 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1065243 1097149 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1097148 1097149 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1097150 1097166 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1097150 1097166 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1097167 1097168 . + . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1097167 1116646 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1116645 1116646 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1116647 1116660 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1116647 1116660 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1116661 1116662 . + . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1116661 1178700 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1178699 1178700 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1178701 1178778 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1178701 1178778 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 160602 1178778 118 + . alignment_id 19 ; Query SPP00000007_1.0 ; Align 160602 2 9 ; Align 257417 6 15 ; Align 263638 12 9 ; Align 415576 16 21 ; Align 423353 24 6 ; Align 462632 27 3 ; Align 501666 29 9 ; Align 588528 33 3 ; Align 700957 35 9 ; Align 790628 39 3 ; Align 829964 41 36 ; Align 863507 54 30 ; Align 899400 65 48 ; Align 959992 82 30 ; Align 992428 93 36 ; Align 1065181 106 60 ; Align 1097151 127 15 ; Align 1116649 133 12 ; Align 1178701 137 78 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 117 Query range: 0 -> 194 Target range: 7744 -> 934268 1 : MetSerLeuGlyArgLeuCysArg >>>> Target Intron 1 >>>> LeuLe : 10 :!:!!!|||||||||:!! ..! 62121 bp !:! ValThrLeuGlyArgValMetGlu++ ++GluMe 7745 : GTCACACTGGGCCGTGTGATGGAGgt.........................agGAAAT : 69893 11 : uLysProAla{Le} >>>> Target Intron 2 >>>> {u}LeuCysGlyAla : 18 !! !||||||{! } 31276 bp {!} |||||| tMetProAla{Tr}++ ++{p}TrpProGlyAla 69894 : GATGCCTGCC{TG}gt.........................ag{g}tggcctggggca : 101193 19 : LeuAlaAlaProGlyLeu{Al} >>>> Target Intron 3 >>>> {a}GlyT : 27 |||:!! !!||| !{ } 25139 bp {!}..!! GlyAlaSerThrGlySer{Ar}++ ++{g}SerA 101194 : ggggcatCCACAGGCAGC{AG}gt.........................ag{G}TCCA : 126359 28 : hrMetCysAlaSerArgAspAspTrpArgCysAlaArgSerMetHisGluPheSer : 46 !..!||| ! ! !..!! !||| !||| !..! !:!!|||...!:::!! rgGlnCysLeuValValSerAlaTrpAlaCysLeuGluValLeuHisSerTrpAla+- 126360 : GGCAGTGTCTTGTCGTCTCTGCCTGGGCCTGCCTAGAGGTCTTGCATAGCTGGGCGgc : 126416 47 : >>>> Target Intron 4 >>>> AlaLysAspIleAspGly >>>> Target : 52 20165 bp |||:!!!!: !..! ! 117 ++AlaGluGluProSerGln++ 126417 : .........................agGCAGAGGAACCCAGCCAGgt........... : 146601 53 : Intron 5 >>>> HisMetValAsnLeuAsp{L} >>>> Target Intron : 58 964 bp |||::::::...:::|||{:} 47449 bp -+HisValLeuGluMetAsp{G}++ 146602 : ..............tgCATGtgctggagatggat{g}gt................... : 264584 59 : 6 >>>> {ys}TyrArgGlyPheValCys{Il} >>>> Target Intron 7 > : 65 {!!} ...||| ||||||{! } 30836 bp ++{lu}ArgAsnGlyArgValCys{Ar}++ 264585 : ......ag{ag}agaaatGGGAGGGTCTGC{AG}gt...................... : 312055 66 : >>> {e}ValThrAsnValAla{Se} >>>> Target Intron 8 >>>> {r : 71 {!} !|||!..!.! !{ } 68090 bp {! ++{g}TyrThrArgAlaTyr{Va}++ ++{l 312056 : ...ag{A}TACACAAGAGCATAC{GT}gt.........................ag{A : 410997 72 : }GlnUnkGlyLysThrGluValAsnTyrThrGlnLeuValAspLeuHisAlaArgTyr : 90 }!!.... ||| :::|||:::||| :::||| }HisAlaIleTrpCysValCysValTyrIleThrTyrIleAspIleHisMetGlnTyr 410998 : }CATgcaatatggtgtgtgtgtgtgtatataacatatatagatatacatatgcaatac : 411052 91 : {A} >>>> Target Intron 9 >>>> {la}GluCysGlyLeuArgIleLeuA : 99 {|} 15772 bp {||}||| !! !:!!!:!|||! !! {A}++ ++{la}GluSerLeuMetLysIleProA 411053 : {g}gt.........................ag{CC}GAAAGCCTCATGAAGATCCCAG : 426851 100 : laPheProCysAsnGln{P} >>>> Target Intron 10 >>>> {he}GlyL : 107 !!!.||||||:!!.!.{ } 25950 bp { }|||: spLeuProCysHisAsn{G}-+ ++{ly}GlyA 426852 : ACTTACCCTGTCATAAT{G}at..........................ag{ga}ggca : 452825 108 : ysGlnGluPro{Gl} >>>> Target Intron 11 >>>> {y}SerAsn >> : 114 :::::||||||{||} 9791 bp {|}|||::: rgGluGluPro{Gl}+- ++{y}SerHis++ 452826 : gagaagagCCG{GG}gg..........................ag{g}agtcatgt.. : 462639 115 : >> Target Intron 12 >>>> GluGluIleLysGluPheAlaAlaGlyTyrAs : 124 63998 bp ...:!!:!!!.. !!!.!..!|||! || ++SerGlnValSerPheValValCysGlySerAs 462640 : ........................agTCCCAAGTCAGCTTCGTCGTCTGTGGGTCAAA : 526665 125 : nValLysPheAspMetPheSerLysIleCysValAsnGlyAsp >>>> Target I : 139 | !!! !||| :!! ||| .!! !.!... !!..! 3999 nPheThrPheArgLeuAlaSerGlyPheThrAlaGlyArgSer++ 526666 : CTTCACATTTAGACTGGCGTCTGGCTTCACGGCTGGAAGATCTgt............. : 526712 140 : ntron 13 >>>> AspAlaHisProLeuTrp{Ly} >>>> Target Intron : 145 6 bp :!:|||||| ! !!::{!:} 29205 bp ++GluAlaHisCysSerPhe{Ar}++ 526713 : .............aggAAGCGCATTGCAGCTTC{AG}gt................... : 566728 146 : 14 >>>> {s}{Tr} >>>> Target Intron 15 >>>> {p}MetLysIle : 149 {!}{||} 5526 bp {|} ... ++{g}{Tr}++ ++{p}GlySerSer 566729 : .......ag{A}{TG}gt..........................ag{g}ggatcttcc : 601467 150 : GlnPro{Ly} >>>> Target Intron 16 >>>> {s}GlyLysGlyIleLeu : 157 ||| {!:} 14446 bp {!}||| ||||||||| GlnSer{Ar}++ ++{g}GlyTrpGlyIleLeu 601468 : caatcc{ag}gt..........................ag{g}ggctgggggataCTG : 615937 158 : {G} >>>> Target Intron 17 >>>> {ly}Asn >>>> Target Intr : 160 {|} 65717 bp {||}.!. 105449 {G}++ ++{ly}Glu+- 615938 : {G}gt..........................ag{GG}GAGgc................ : 681665 161 : on 18 >>>> AlaIleLysTrpAsnPheThrLysPheLeuIle{As} >>>> Ta : 171 bp !|||:!!!::||||||..!|||!:: ! !{ !} ++LeuIleGluTyrAsnPheValLysTrpAlaGly{Ly}-+ 681666 : ..........agCTCATTGAATATAATTTTGTTAAGTGGGCAGGA{AA}at....... : 787149 172 : rget Intron 19 >>>> {p}LysAsnGlyCysValValLys >>>> Target : 179 52448 bp { }|||!..|||.....!:!! ! 94 ++{s}LysThrGlyAlaThrIleHis++ 787150 : ...................ag{G}AAAACAGGTGCAACTATCCATgt........... : 839619 180 : Intron 20 >>>> ArgTyrGlyProMetGluGluProLeuValIleGluLysAs : 192 604 bp !:!|||! !|||:!: ..!||| !||| !|||..... ++LysTyrAspProLeuProArgProAlaValSerGluSerSe 839620 : ...............agAAGTATGACCCTCTCCCCCGACCCGCAGTCTCAGAGTCTTC : 934260 193 : pLeuPro : 194 !! !||| rProPro 934261 : CCCACCT : 934268 vulgar: SPP00000007_1.0 0 194 . NIVO01030317.1:subseq(555646,1182752) 7744 934268 + 117 M 8 24 5 0 2 I 0 62117 3 0 2 M 5 15 S 0 2 5 0 2 I 0 31272 3 0 2 S 1 1 M 10 30 S 0 2 5 0 2 I 0 25135 3 0 2 S 1 1 M 20 60 5 0 2 I 0 20161 3 0 2 M 6 18 5 0 2 I 0 117960 3 0 2 M 6 18 S 0 1 5 0 2 I 0 47445 3 0 2 S 1 2 M 6 18 S 0 2 5 0 2 I 0 30832 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 68086 3 0 2 S 1 1 M 19 57 S 0 1 5 0 2 I 0 15768 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 25946 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 9787 3 0 2 S 1 1 M 2 6 5 0 2 I 0 63994 3 0 2 M 25 75 5 0 2 I 0 39992 3 0 2 M 6 18 S 0 2 5 0 2 I 0 29201 3 0 2 S 1 3 5 0 2 I 0 5522 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 14442 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 65713 3 0 2 S 1 2 M 1 3 5 0 2 I 0 105445 3 0 2 M 11 33 S 0 2 5 0 2 I 0 52444 3 0 2 S 1 1 M 7 21 5 0 2 I 0 94600 3 0 2 M 16 48 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 7745 934268 117 + . gene_id 20 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 7745 7768 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 7745 7768 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 7769 7770 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 7769 69889 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 69888 69889 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 69890 69906 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 69890 69906 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 69907 69908 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 69907 101182 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 101181 101182 . + . intron_id 1 ; splice_site "Ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 101183 101215 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 101183 101215 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 101216 101217 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 101216 126354 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 126353 126354 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 126355 126415 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 126355 126415 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 126416 126417 . + . intron_id 4 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 126416 146580 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146579 146580 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146581 146598 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146581 146598 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146599 146600 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146599 264562 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 264561 264562 . + . intron_id 4 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 264563 264581 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 264563 264581 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 264582 264583 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 264582 312030 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 312029 312030 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 312031 312052 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 312031 312052 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 312053 312054 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 312053 342888 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 342887 342888 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 342889 342906 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 342889 342906 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 342907 342908 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 342907 410996 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 410995 410996 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 410997 411055 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 410997 411055 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 411056 411057 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 411056 426827 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 426826 426827 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 426828 426869 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 426828 426869 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 426870 426871 . + . intron_id 10 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 426870 452819 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 452818 452819 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 452820 452838 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 452820 452838 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 452839 452840 . + . intron_id 11 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 452839 462629 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462636 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462636 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462637 462638 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462637 526634 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 526633 526634 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 526635 526709 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 526635 526709 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 526710 526711 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 526710 566705 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 566704 566705 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 566706 566725 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 566706 566725 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 566726 566727 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 566726 595930 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 595929 595930 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 595931 595933 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 595931 595933 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 595934 595935 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 595934 601459 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 615923 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 615922 615923 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 615924 615940 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 615924 615940 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 615941 615942 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 615941 681657 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 681656 681657 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 681658 681662 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 681658 681662 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 681663 681664 . + . intron_id 18 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 681663 787111 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 787110 787111 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 787112 787146 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 787112 787146 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 787147 787148 . + . intron_id 19 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 787147 839594 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 839593 839594 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 839595 839616 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 839595 839616 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 839617 839618 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 839617 934220 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 934219 934220 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 934221 934268 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 934221 934268 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 7745 934268 117 + . alignment_id 20 ; Query SPP00000007_1.0 ; Align 7745 1 24 ; Align 69890 9 15 ; Align 101184 15 30 ; Align 126356 26 60 ; Align 146581 46 18 ; Align 264563 52 18 ; Align 312033 59 18 ; Align 342890 66 15 ; Align 410998 72 57 ; Align 426830 92 39 ; Align 452822 106 15 ; Align 462631 112 6 ; Align 526635 114 75 ; Align 566706 139 18 ; Align 601461 147 15 ; Align 615925 153 15 ; Align 681660 159 3 ; Align 787112 160 33 ; Align 839596 172 21 ; Align 934221 179 48 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 113 Query range: 1 -> 195 Target range: 3015 -> 1166704 2 : SerLeuGlyArgLeuCysArgLeuLeu{L} >>>> Target Intron 1 >>> : 11 .!!|||! ! !|||||| !!|||! !{ } 6059 bp GlyLeuAspSerLeuCysGlyLeuGln{A}++ 3016 : GGCTTGGATTCCCTCTGCGGCCTCCAG{G}gt........................ : 3046 12 : > {ys}ProAlaLeuLeuCysGlyAlaLeu{Al} >>>> Target Intron : 20 { !}||| !! !|||||| !!..!! !{ } 40782 bp ++{la}ProArgCysLeuCysTrpCysSer{Ar}++ 3047 : .ag{CA}CCGCGCTGCTTGTGCTGGTGCTCA{AG}gt................... : 9133 21 : 2 >>>> {a}AlaProGlyLeu{A} >>>> Target Intron 3 >>>> { : 25 {!} !|||||||||{|} 103842 bp { ++{g}TyrProGlyLeu{A}+- ++{ 9134 : ......ag{G}TACCCTGGGCTG{G}gc.........................ag{ : 153769 26 : la}GlyThrMetCysAlaSer >>>> Target Intron 4 >>>> ArgAsp : 33 ||}..!|||! ||| !!..! 21418 bp |||!!: la}SerThrThrCysProGlu++ ++ArgGlu 153770 : CC}TCTACCACCTGCCCCGAGgt.........................agAGGGAG : 175210 34 : >>>> Target Intron 5 >>>> AspTrpArgCysAlaArgSerMetHis : 42 109484 bp ||| !||| !||| !!! ! ! ++ ++ThrTrpPheCysTrpArgProThrGly 175211 : gt.........................agACGTGGTTCTGCTGGCGGCCCACGGGC : 284721 43 : {Gl} >>>> Target Intron 6 >>>> {u}PheSerAlaLysAspIleAs : 50 { } 94149 bp {!} |||:::::: || {Tr}++ ++{p}GlySerSerArgProArgAs 284722 : {TG}gt.........................ag{g}ggatcgtcccgacccaggga : 378894 51 : pGlyHisMetValAsnLeuAspLysTyrArgGly{Ph} >>>> Target Intr : 62 |||| |||::: ... |||{ } 41084 b pGlyThrArgValSerCysValSerCysIleGly{Ar}++ 378895 : tggaacccgggtctcttgtgtctcctgcattggc{ag}gt................ : 378934 63 : on 7 >>>> {e}ValCysIleValThrAsnValAlaSerGln >>>> Targe : 73 p { } !|||:!:|||.!! !|||!.! !||| 1 ++{g}HisCysLeuValAlaCysValGlyHisGln++ 378935 : .........ag{G}CACTGCCTGGTCGCCTGTGTCGGACACCAAgt.......... : 420049 74 : t Intron 8 >>>> UnkGlyLysThr{G} >>>> Target Intron 9 > : 77 1730 bp |||:!!|||{|} 62577 bp ++GlyGlyGlnThr{G}-+ 420050 : ...............agggtggacAGACA{G}at...................... : 431792 78 : >>> {lu}ValAsnTyrThr >>>> Target Intron 10 >>>> GlnLe : 83 {||}||| !:!||| 59942 bp :!!|| ++{lu}ValAlaPheThr++ ++GluLe 431793 : ...ag{AG}GTGGCGTTCACGgt..........................agGAGCT : 554326 84 : u{Va} >>>> Target Intron 11 >>>> {l}AspLeuHis{Al} >>> : 88 |{||} 18637 bp {|} ! ||||||{ } u{Va}++ -+{l}LysLeuHis{Ar}++ 554327 : G{GT}gt..........................tg{G}AAACTACAC{AG}gt... : 572982 89 : > Target Intron 12 >>>> {a}ArgTyrAlaGluCysGlyLeuArgIleL : 98 103343 bp {!}... |||... ......||| ++{g}GluGluAlaSerPheAsnPheArgAlaS 572983 : .......................ag{a}gaggaagcgtctttcaatttcagggctt : 676351 99 : euAlaPheProCysAsnGlnPheGlyLysGlnGlu{P} >>>> Target Intr : 110 |||::::::|||||| |||:::{ } 25036 b erLeuThrIleCysSerGluPheGlyAlaGlnAsp{S}++ 676352 : cactcaccatctgcagtgaatttggagcccaagat{a}gt................ : 676390 111 : on 13 >>>> {ro}GlySerAsn{G} >>>> Target Intron 14 >>>> : 114 p { !}||||||:::{|} 107828 bp ++{er}GlySerSer{G}++ 676391 : ..........ag{GC}GGTAGCTCA{g}gt.......................... : 701438 115 : {lu}GluIleLysGluPheAlaAlaGlyTyrAsnValLysPheAspMetPheSe : 131 {||}... !|||..!||| ! !|||!::||| ! ! ::: |||.. ++{lu}SerProLysSerPheLeu***GlyTrpAsn***AlaGlyAsnTyrPheLy 701439 : ag{AG}TCTCCAAAGTCATTTTTATGAGGTTGGAACTGAGCGgggaattattttaa : 809314 132 : r >>>> Target Intron 15 >>>> LysIleCysValAsnGlyAspAspA : 140 . 776 bp ||| ... |||| s+- ++LysAlaGluHisGlnArgThrAspA 809315 : agc..........................agaaagctgagcaccaaagaactgatg : 810117 141 : laHisProLeuTrpLysTrpMetLysIle >>>> Target Intron 16 >>> : 150 || |||||| ||| :::::: 22380 bp laPheGluLeuTrpHisTrpArgArgLeu-+ 810118 : cttttgaactgtggcattggagaagactctt......................... : 810149 151 : > GlnProLysGlyLysGlyIle{Le} >>>> Target Intron 17 >>>> : 157 ...||| |||::!|||! !{ } 31223 bp ++AsnProMetGlyArgGlyThr{Gl}++ 810150 : .agaatcccatgggaaGAGGAACC{GG}gt.......................... : 832552 158 : {u}GlyAsnAlaIleLys{T} >>>> Target Intron 18 >>>> {rp : 163 {!} !|||:!!||| !{ } 2484 bp {!! ++{y}***AsnSerIlePro{G}++ ++{ly 832553 : ag{A}TAAAATTCAATTCCA{G}gt..........................ag{gg : 866274 164 : }AsnPheThr >>>> Target Intron 19 >>>> LysPheLeuIleAspL : 172 }:::||| 116022 bp |||:!: ! }SerPheGln++ ++GlyGluLeuValArgV 866275 : }tcttttcaagt..........................agggTGAGCTGGTGCGTG : 982322 173 : ysAsnGlyCysValValLys{A} >>>> Target Intron 20 >>>> {rg : 179 !.!. !!|||..! !|||{ } 167292 bp {!! alGlnArgCysThrProLys{G}++ ++{ly 982323 : TGCAGAGGTGTACACCTAAG{G}gt..........................ag{GA : 1149636 180 : }TyrGlyProMet{G} >>>> Target Intron 21 >>>> {lu}GluPro : 186 } !||||||.!.{|} 17019 bp {||} ! ! }ArgGlyProPhe{G}++ ++{lu}ThrGln 1149637 : }CGTGGCCCATTC{G}gt..........................ag{AG}ACTCAG : 1166675 187 : LeuValIleGluLysAspLeuProHis : 195 :!!:!!! !... ! !! !|||||| IleLeuThrSerLeuThrArgProHis 1166676 : ATCCTCACTTCCCTGACCCGGCCACAC : 1166704 vulgar: SPP00000007_1.0 1 195 . NIVO01030317.1:subseq(555646,1182752) 3015 1166704 + 113 M 9 27 S 0 1 5 0 2 I 0 6055 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 40778 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 103838 3 0 2 S 1 2 M 6 18 5 0 2 I 0 21414 3 0 2 M 2 6 5 0 2 I 0 109480 3 0 2 M 9 27 S 0 2 5 0 2 I 0 94145 3 0 2 S 1 1 M 18 54 S 0 2 5 0 2 I 0 41080 3 0 2 S 1 1 M 10 30 5 0 2 I 0 11726 3 0 2 M 4 12 S 0 1 5 0 2 I 0 62573 3 0 2 S 1 2 M 4 12 5 0 2 I 0 59938 3 0 2 M 2 6 S 0 2 5 0 2 I 0 18633 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 103339 3 0 2 S 1 1 M 21 63 S 0 1 5 0 2 I 0 25032 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 107824 3 0 2 S 1 2 M 17 51 5 0 2 I 0 772 3 0 2 M 18 54 5 0 2 I 0 22376 3 0 2 M 7 21 S 0 2 5 0 2 I 0 31219 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 2480 3 0 2 S 1 2 M 3 9 5 0 2 I 0 116018 3 0 2 M 12 36 S 0 1 5 0 2 I 0 167288 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 17015 3 0 2 S 1 2 M 11 33 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 3016 1166704 113 + . gene_id 21 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 3016 3043 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 3016 3043 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 3044 3045 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 3044 9102 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 9101 9102 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 9103 9130 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 9103 9130 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 9131 9132 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 9131 49912 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 49911 49912 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 49913 49926 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 49913 49926 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 49927 49928 . + . intron_id 3 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 49927 153768 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 153767 153768 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 153769 153788 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 153769 153788 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 153789 153790 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 153789 175206 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 175205 175206 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 175207 175212 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 175207 175212 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 175213 175214 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 175213 284696 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 284695 284696 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 284697 284725 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 284697 284725 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 284726 284727 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 284726 378874 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 378873 378874 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 378875 378931 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 378875 378931 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 378932 378933 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 378932 420015 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 420014 420015 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 420016 420046 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 420016 420046 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 420047 420048 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 420047 431776 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 431775 431776 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 431777 431789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 431777 431789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 431790 431791 . + . intron_id 9 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 431790 494366 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 494365 494366 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 494367 494380 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 494367 494380 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 494381 494382 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 494381 554322 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 554321 554322 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 554323 554330 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 554323 554330 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 554331 554332 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 554331 572967 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 572966 572967 . + . intron_id 10 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 572968 572979 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 572968 572979 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 572980 572981 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 572980 676322 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 676321 676322 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 676323 676387 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 676323 676387 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 676388 676389 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 676388 701423 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 701422 701423 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 701424 701435 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 701424 701435 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 701436 701437 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 701436 809263 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 809262 809263 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 809264 809316 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 809264 809316 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 809317 809318 . + . intron_id 15 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 809317 810092 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 810091 810092 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 810093 810146 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 810093 810146 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 810147 810148 . + . intron_id 16 ; splice_site "tt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 810147 832526 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 832525 832526 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 832527 832549 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 832527 832549 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 832550 832551 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 832550 863772 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 866273 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 866272 866273 . + . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 866274 866284 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 866274 866284 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 866285 866286 . + . intron_id 19 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 866285 982306 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 982305 982306 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 982307 982343 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 982307 982343 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 982344 982345 . + . intron_id 20 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 982344 1149635 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1149634 1149635 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1149636 1149650 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1149636 1149650 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1149651 1149652 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1149651 1166669 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1166668 1166669 . + . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1166670 1166704 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1166670 1166704 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 3016 1166704 113 + . alignment_id 21 ; Query SPP00000007_1.0 ; Align 3016 2 27 ; Align 9105 12 24 ; Align 49914 21 12 ; Align 153771 26 18 ; Align 175207 32 6 ; Align 284697 34 27 ; Align 378876 44 54 ; Align 420017 63 30 ; Align 431777 73 12 ; Align 494369 78 12 ; Align 554323 82 6 ; Align 572969 85 9 ; Align 676324 89 63 ; Align 701426 111 9 ; Align 809266 115 51 ; Align 810093 132 54 ; Align 832527 150 21 ; Align 863774 158 15 ; Align 866276 164 9 ; Align 982307 167 36 ; Align 1149638 180 12 ; Align 1166672 185 33 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 112 Query range: 12 -> 196 Target range: 8134 -> 778867 13 : AlaLeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAlaGlyThrMetCysAlaSerA : 32 :!!|||! ! !!|||::! |||... ||| ||||||||| !!! !| SerLeuTrpGlyGlySerArgAlaGlyGlyGlyGlyLeuGlyThrMetProProIleA 8135 : TCCTTGTGGGGCGGAAGCagagcgggggggggggggctaggTACCATGCCACCTATCA : 8192 33 : rgAspAspTrp{Ar} >>>> Target Intron 1 >>>> {g}CysAlaArg{S : 40 ||||| ! ! {||} 8692 bp {|} !!..!|||{| rgAspLeuGly{Ar}++ ++{g}SerCysArg{S 8193 : GAGATCTTGGT{AG}gt.........................ag{A}AGTTGCAGA{A : 16908 41 : e} >>>> Target Intron 2 >>>> {r}MetHisGluPheSerAlaLysAsp : 48 |} 11242 bp {|}:!: !|||||| ! ! !!: e}++ ++{r}ValSerGluPheLeuMetGlyGlu 16909 : G}gt.........................ag{T}GTATCCGAGTTCCTCATGGGTGAA : 28174 49 : IleAspGlyHisMetVal{As} >>>> Target Intron 3 >>>> {n}LeuA : 57 :!:|||.!! !!!::!!{ } 33458 bp { } !! ValAspSerSerIleLeu{Le}++ ++{u}GlyA 28175 : GTGGACAGCAGCATCCTC{CT}gt.........................ag{G}GGAG : 61659 58 : spLysTyrArgGly >>>> Target Intron 4 >>>> PheValCysIleVal : 66 !..|||||| ! 2517 bp ||| |||::: laSerTyrArgGln++ ++PheProCysLeu*** 61660 : CAAGTTACAGACAAgt.........................agtttccctgtctgtaa : 64203 67 : ThrAsnValAlaSerGlnUnkGlyLysThrGluValAsnTyr{Th} >>>> Targe : 81 ...... !..!||||||...||| ! !...!.!:!! !{! } 2 AsnArgProCysSerGlnAlaGlyProAspSerAlaHisSer{Ly}++ 64204 : aatagACCATGCTCCCAAGCTGGCCCAGACTCTGCACACAGC{AA}gt.......... : 64252 82 : t Intron 5 >>>> {r}GlnLeuValAspLeuHisAlaArgTyrAlaGluCysGl : 94 0170 bp {!}|||||||||... ...||| ! ||||| ++{s}GlnLeuValSerArgThrGlyArgValAsnProCysGl 64253 : ...............ag{a}cagctggtctccagaactgggagagtaAATCCCTGTGG : 84457 95 : yLeu{Ar} >>>> Target Intron 6 >>>> {g}IleLeuAlaPhe >>>> : 101 ||||{||} 6413 bp {|}|||! !||| yLeu{Ar}-+ ++{g}IleHisAlaLys++ 84458 : TTTA{AG}tt.........................ag{G}ATTCATGCCAAGgt.... : 90893 102 : Target Intron 7 >>>> ProCysAsnGln >>>> Target Intron 8 : 105 34104 bp !!|||::::!! 33408 bp ++ThrCysSerGlu++ 90894 : .....................agACCTGCTCAGAGgt..................... : 125009 106 : >>>> PheGlyLysGlnGluProGlySerAsnGluGluIleLysGlu{Ph} >>>> : 119 ||| !:!!! .!.||||||.!!!..|||:!!:!::!!...{ } ++PheGlnGluProHisProGlyGlyArgGluGlnValGlnSer{Ar}+- 125010 : ....agTTCCAGGAGCCCCACCCAGGGGGAAGGGAACAGGTGCAGTCC{AG}ga.... : 158461 120 : Target Intron 9 >>>> {e}AlaAlaGlyTyrAsnValLysPheAspMetPh : 130 10583 bp { }::!!.!||||||...! !... ||| !: ++{g}SerGlyGlyTyrGlyGlySerArgAspTyrTy 158462 : .....................ag{G}AGTGGGGGCTATGGAGGCTCCAGAGACTACTA : 169073 131 : eSer{Ly} >>>> Target Intron 10 >>>> {s}IleCysValAsnGlyAs : 138 !|||{!.} 2690 bp {.}! !||| !!!.!||| ! rSer{Se}++ ++{r}ArgCysPheThrGlyLy 169074 : CAGC{AG}gt..........................ag{C}AGATGTTTTACTGGCAA : 171787 139 : p >>>> Target Intron 11 >>>> AspAlaHis{Pr} >>>> Target : 142 79630 bp ||| !|||{ } 131 s++ ++AspLysHis{Va}++ 171788 : Ggt..........................agGACAAACac{gt}gt............ : 251433 143 : Intron 12 >>>> {o}LeuTrpLysTrpMet >>>> Target Intron 13 : 148 35 bp {!} |||:::|||||| 185074 bp +-{l}CysTrpArgTrpMet++ 251434 : ..............at{G}tgctggagatggatggt...................... : 264584 149 : >>>> LysIleGlnProLysGlyLysGlyIle{Le} >>>> Target Intron : 157 ! ||||||::: ! |||:!:{ } 39980 bp ++AlaTrpGlnProArgLeuCysGlyLeu{Gl}++ 264585 : ....agGCGTGGCAACCCCGCCTCTGCGGTTTG{GG}gt................... : 449687 158 : 14 >>>> {u}GlyAsnAlaIleLysTrpAsnPheThrLysPheLeuIleAspLysA : 173 {!} ..! !:!!...!! ::!! ! !...! !! !:!!! |||. ++{y}GluGlyPheLeuSerCysSerCysLeuSerSerProLeuGlyLysG 449688 : .......ag{a}gaaGGTTTTCTCTCCTGTTCTTGCCTTTCCTCTCCACTTGGGAAGG : 489711 174 : snGlyCysValValLysArg{T} >>>> Target Intron 15 >>>> {yr}G : 181 !.|||||| !:!!..! !{!} 12843 bp { !}. luGlyCysTyrLeuSerVal{S}++ ++{er}S 489712 : AAGGCTGCTACCTTTCAGTA{T}gt..........................ag{CC}T : 502578 182 : lyProMet >>>> Target Intron 16 >>>> >>>> Target Intron : 184 .!|||! ! 164071 bp 112171 bp erProArg++ ++++ 502579 : CCCCCAGGgt..........................aggt.................. : 666660 185 : 17 >>>> GluGluProLeuValIleGluLysAspLeuProHisTyr : 196 ..!! |||||| !:!:.!.!:!||||||||| !!:! ++ArgAlaProLeuCysValHisArgAspLeuProAlaPhe 666661 : ........agCGAGCCCCCCTGTGTGTGCACAGGGATCTGCCTGCTTTC : 778867 vulgar: SPP00000007_1.0 12 196 . NIVO01030317.1:subseq(555646,1182752) 8134 778867 + 112 M 23 69 S 0 2 5 0 2 I 0 8688 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 11238 3 0 2 S 1 1 M 14 42 S 0 2 5 0 2 I 0 33454 3 0 2 S 1 1 M 6 18 5 0 2 I 0 2513 3 0 2 M 19 57 S 0 2 5 0 2 I 0 20166 3 0 2 S 1 1 M 14 42 S 0 2 5 0 2 I 0 6409 3 0 2 S 1 1 M 4 12 5 0 2 I 0 34100 3 0 2 M 4 12 5 0 2 I 0 33404 3 0 2 M 14 42 S 0 2 5 0 2 I 0 10579 3 0 2 S 1 1 M 12 36 S 0 2 5 0 2 I 0 2686 3 0 2 S 1 1 M 6 18 5 0 2 I 0 79626 3 0 2 M 3 9 S 0 2 5 0 2 I 0 13131 3 0 2 S 1 1 M 5 15 5 0 2 I 0 185070 3 0 2 M 9 27 S 0 2 5 0 2 I 0 39976 3 0 2 S 1 1 M 22 66 S 0 1 5 0 2 I 0 12839 3 0 2 S 1 2 M 3 9 5 0 2 I 0 164067 3 0 2 5 0 2 I 0 112167 3 0 2 M 13 39 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 8135 778867 112 + . gene_id 22 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 8135 8205 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 8135 8205 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 8206 8207 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 8206 16897 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 16896 16897 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 16898 16909 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 16898 16909 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 16910 16911 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 16910 28151 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 28150 28151 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 28152 28196 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 28152 28196 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 28197 28198 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 28197 61654 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 61653 61654 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 61655 61673 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 61655 61673 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 61674 61675 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 61674 64190 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64189 64190 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64191 64249 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64191 64249 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64250 64251 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64250 84419 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 84418 84419 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 84420 84464 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 84420 84464 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 84465 84466 . + . intron_id 6 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 84465 90877 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 90876 90877 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 90878 90890 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 90878 90890 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 90891 90892 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 90891 124994 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 124993 124994 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 124995 125006 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 124995 125006 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 125007 125008 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 125007 158414 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 158413 158414 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 158415 158458 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 158415 158458 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 158459 158460 . + . intron_id 9 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 158459 169041 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 169040 169041 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 169042 169080 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 169042 169080 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 169081 169082 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 169081 171770 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171769 171770 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171771 171789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171771 171789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171790 171791 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171790 251419 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 251418 251419 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 251420 251430 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 251420 251430 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 251431 251432 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 251431 264565 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 264564 264565 . + . intron_id 11 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 264566 264581 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 264566 264581 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 264582 264583 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 264582 449655 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 449654 449655 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 449656 449684 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 449656 449684 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 449685 449686 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 449685 489664 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 489663 489664 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 489665 489732 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 489665 489732 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 489733 489734 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 489733 502575 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 502574 502575 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 502576 502586 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 502576 502586 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 502587 502588 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 502587 666657 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 778828 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 778827 778828 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 778829 778867 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 778829 778867 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 8135 778867 112 + . alignment_id 22 ; Query SPP00000007_1.0 ; Align 8135 13 69 ; Align 16899 37 9 ; Align 28153 41 42 ; Align 61656 56 18 ; Align 64191 62 57 ; Align 84421 82 42 ; Align 90879 97 12 ; Align 124995 101 12 ; Align 158415 105 42 ; Align 169043 120 36 ; Align 171772 133 18 ; Align 251420 139 9 ; Align 264567 143 15 ; Align 449656 148 27 ; Align 489666 158 66 ; Align 502578 181 9 ; Align 778829 184 39 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 112 Query range: 3 -> 183 Target range: 40513 -> 1162328 4 : GlyArgLeuCysArgLeuLeu{Ly} >>>> Target Intron 1 >>>> {s : 11 ||||||||| !||| {!:} 84458 bp {! GlyArgLeuLeuPheLeuLys{Ar}++ ++{g 40514 : GGACGTCTGCTGTTCCTAAAa{ag}gt.........................ag{A : 124995 12 : }ProAlaLeu{Le} >>>> Target Intron 2 >>>> {u}CysGlyAlaL : 19 }||||||! !{ } 2022 bp {!}! !|||::! }ProAlaGln{Ar}++ ++{g}SerGlySerT 124996 : }CCTGCTCAG{AG}gt.........................ag{A}TCCGGCAGCA : 127039 20 : eu{A} >>>> Target Intron 3 >>>> {la}{Al} >>>> Target : 21 !{|} 44018 bp {||}{::} 148 hr{A}++ ++{la}{Se}++ 127040 : CA{G}gt.........................ag{CC}{AG}gt............ : 171067 22 : Intron 4 >>>> {a}Pro{G} >>>> Target Intron 5 >>>> {ly : 23 32 bp {!}|||{|} 3832 bp {|| ++{r}Pro{G}++ ++{ly 171068 : .............ag{c}cca{g}gt.........................ag{GG : 189734 24 : }LeuAlaGly >>>> Target Intron 6 >>>> ThrMetCysAla{S} : 31 }! !|||||| 33297 bp !:!!||||||{|} }ProAlaGly++ -+AspLeuCysAla{S}++ 189735 : }CCTGCTGGGgt.........................tggaTCTGTGTGCG{T}gt : 223055 32 : >>>> Target Intron 7 >>>> {er}ArgAspAspTrpArgCysAla{Ar} : 39 82072 bp {||} !!|||||||||! ! !!{||} ++{er}***AspAspTrpProLeuPro{Ar} 223056 : .........................ag{CC}TGAGATGATTGGCCACTACCT{AG} : 305150 40 : >>>> Target Intron 8 >>>> {g}SerMet >>>> Target Intr : 42 157478 bp {|}||| 107427 b +- ++{g}SerHis++ 305151 : gc.........................ag{g}agtcatgt................ : 462639 43 : on 9 >>>> HisGluPheSerAlaLysAspIleAspGlyHis{Me} >>>> T : 53 p !:!! |||||| !||| !|||{ } ++PheGlnGlySerAlaPheProArgAspProHis{Gl}++ 462640 : .........agTTTCAGGGGAGCGCCTTTCCACGAGACCCTCAC{GG}gt...... : 570101 54 : arget Intron 10 >>>> {t}ValAsnLeuAspLysTyrArgGlyPheValC : 64 46028 bp { }:!!::!! !!!:::: !! ! !|||..!. ++{y}LeuSer***GluArgSerLeuIlePheThrA 570102 : ....................ag{T}CTGTCTTAAGAACGCAGCCTGATCTTTACAG : 616158 65 : ysIleValThrAsnValAlaSerGlnUnkGlyLysThrGluValAsnTyr{T} > : 81 .!||| ! !! ..!|||||| ! !!:!!.!!|||:!!||| { } laIleSerProProThrAlaSerGlyArgArgGluAlaGluIleAsnArg{G}+- 616159 : CCATCTCCCCTCCAACAGCCTCAGGACGTAGGGAAGCTGAAATCAACAGA{G}gg. : 616212 82 : >>> Target Intron 11 >>>> {hr}GlnLeuValAspLeuHisAlaArgT : 90 39945 bp { !}:!! !|||..! !|||:!!|||! ++{ly}LysGlyValSerArgHisSerArgP 616213 : .........................ag{GA}AAGGGAGTCTCTAGACACTCACGCT : 656181 91 : yrAlaGlu >>>> Target Intron 12 >>>> >>>> Target Intr : 93 :! !:!! 10468 bp 90185 b heLeuGln++ ++++ 656182 : TTCTGCAGgt..........................aggt................ : 666660 94 : on 13 >>>> CysGlyLeuArgIleLeuAlaPheProCys{As} >>>> Tar : 103 p ...! !|||!:!|||:!!::!|||! !|||{!:} ++AlaAspLeuLysIleValSerPheLeuCys{Se}++ 666661 : ..........agGCAGATTTGAAAATTGTTAGTTTTCTTTGC{AG}gt........ : 756877 104 : get Intron 14 >>>> {n}GlnPheGlyLysGlnGluProGlySerAsnGlu : 114 216 bp {!} ...|||...... ||||||||| ::: ++{r}ProLeuGlySerSerValProGlySerLeuGln 756878 : ..................ag{c}ccgctgggctcctctgtccctgggagtctccag : 757122 115 : >>>> Target Intron 15 >>>> GluIleLysGluPheAlaAlaGlyTy : 123 105795 bp ||||||...!!:! ! !!|||! !!: +- ++GluIleSerAspSerProAlaGluPh 757123 : gc..........................agGAGATTTCCGATTCCCCTGCAGAGTT : 862944 124 : rAsnValLysPheAspMetPheSerLysIleCysValAsnGlyAspAspAlaHisP : 142 !...|||!:! !..! ! .!! ! ||| ! !!.!.!.|||!.!! !| eGlyValArgGlySerArg***GlyPheThrCysArgLeuAlaGlnAspGlyLeuP 862945 : CGGAGTTAGAGGTTCCCGCTGAGGCTTTACGTGCCGGCTCGCACAAGACGGGCTTC : 863001 143 : roLeuTrpLys >>>> Target Intron 16 >>>> TrpMetLys >>>> : 149 ||:!!||| ! 91532 bp |||:!!!:! roValTrpAla++ -+TrpLeuArg++ 863002 : CAGTCTGGGCGgt..........................cgTGGCTGAGGgt.... : 954556 150 : Target Intron 17 >>>> IleGlnProLysGlyLysGlyIleLeuGlyAs : 159 33133 bp ::! ! ! ! ||| |||||||||!.!.. ++LeuAlaLeuHisGlyLeuGlyIleLeuAlaGl 954557 : ......................agttAGCTCTGCACGGTCTTGGCATCCTTGCCGG : 987717 160 : nAlaIleLys >>>> Target Intron 18 >>>> >>>> Target In : 163 ! ! ::! 23648 bp 10077 yArgGlyArg+- ++++ 987718 : CCGGGGGCGGgg..........................aggt.............. : 1011379 164 : tron 19 >>>> >>>> Target Intron 20 >>>> Trp{As} >>> : 164 1 bp 27033 bp |||{!!} ++++ ++Trp{Ly}++ 1011380 : ............aggt..........................agtgg{aa}gt... : 1139188 165 : > Target Intron 21 >>>> {n}PheThrLysPhe{Le} >>>> Targe : 169 17061 bp {.}...::: |||{ } ++{s}MetSerGlyPhe{Ar}++ 1139189 : .......................ag{g}atgtctggcttt{ag}gt.......... : 1156264 170 : t Intron 22 >>>> {u}IleAspLysAsnGlyCysValValLysArgTyrGl : 181 6024 bp {!}:!!.!.:!!..!|||..!!.! ! ||| !|| ++{g}ValGlnGluGlyGlyAlaAlaCysProArgGlyGl 1156265 : ................ag{G}GTACAGGAAGGCGGGGCCGCCTGCCCCCGAGGTGG : 1162320 182 : yProMet : 183 ||||:!! yProLeu 1162321 : GCCCTTG : 1162328 vulgar: SPP00000007_1.0 3 183 . NIVO01030317.1:subseq(555646,1182752) 40513 1162328 + 112 M 7 21 S 0 2 5 0 2 I 0 84454 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 2018 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 44014 3 0 2 S 1 4 5 0 2 I 0 14828 3 0 2 S 1 1 M 1 3 S 0 1 5 0 2 I 0 3828 3 0 2 S 1 2 M 3 9 5 0 2 I 0 33293 3 0 2 M 4 12 S 0 1 5 0 2 I 0 82068 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 157474 3 0 2 S 1 1 M 2 6 5 0 2 I 0 107423 3 0 2 M 11 33 S 0 2 5 0 2 I 0 46024 3 0 2 S 1 1 M 27 81 S 0 1 5 0 2 I 0 39941 3 0 2 S 1 2 M 11 33 5 0 2 I 0 10464 3 0 2 5 0 2 I 0 90181 3 0 2 M 10 30 S 0 2 5 0 2 I 0 212 3 0 2 S 1 1 M 11 33 5 0 2 I 0 105791 3 0 2 M 31 93 5 0 2 I 0 91528 3 0 2 M 3 9 5 0 2 I 0 33129 3 0 2 M 14 42 5 0 2 I 0 23644 3 0 2 5 0 2 I 0 100767 3 0 2 5 0 2 I 0 27029 3 0 2 M 1 3 S 0 2 5 0 2 I 0 17057 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 6020 3 0 2 S 1 1 M 14 42 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 40514 1162328 112 + . gene_id 23 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 40514 40536 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 40514 40536 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 40537 40538 . + . intron_id 1 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 40537 124994 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 124993 124994 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 124995 125006 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 124995 125006 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 125007 125008 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 125007 127028 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 127027 127028 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 127029 127042 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 127029 127042 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 127043 127044 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 127043 171060 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 185896 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 185895 185896 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 185897 185901 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 185897 185901 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 185902 185903 . + . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 185902 189733 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 189732 189733 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 189734 189744 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 189734 189744 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 189745 189746 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 189745 223041 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 223040 223041 . + . intron_id 5 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 223042 223054 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 223042 223054 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 223055 223056 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 223055 305126 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 305125 305126 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 305127 305151 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 305127 305151 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 305152 305153 . + . intron_id 8 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 305152 462629 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462636 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462636 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462637 462638 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462637 570063 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 570062 570063 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 570064 570098 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 570064 570098 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 570099 570100 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 570099 616126 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 616125 616126 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 616127 616209 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 616127 616209 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 616210 616211 . + . intron_id 11 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 616210 656154 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 656153 656154 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 656155 656189 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 656155 656189 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 656190 656191 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 656190 666657 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 756842 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 756841 756842 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 756843 756874 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 756843 756874 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 756875 756876 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 756875 757090 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 757089 757090 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 757091 757124 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 757091 757124 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 757125 757126 . + . intron_id 15 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 757125 862919 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 862918 862919 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 862920 863012 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 862920 863012 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863013 863014 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863013 954544 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 954543 954544 . + . intron_id 15 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 954545 954553 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 954545 954553 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 954554 954555 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 954554 987686 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 987685 987686 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 987687 987728 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 987687 987728 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 987729 987730 . + . intron_id 18 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 987729 1011376 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1112147 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1139180 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1139179 1139180 . + . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1139181 1139185 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1139181 1139185 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1139186 1139187 . + . intron_id 21 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1139186 1156246 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1156245 1156246 . + . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1156247 1156261 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1156247 1156261 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1156262 1156263 . + . intron_id 22 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1156262 1162285 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1162284 1162285 . + . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1162286 1162328 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1162286 1162328 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 40514 1162328 112 + . alignment_id 23 ; Query SPP00000007_1.0 ; Align 40514 4 21 ; Align 124996 12 9 ; Align 127030 16 12 ; Align 185898 22 3 ; Align 189736 24 9 ; Align 223042 27 12 ; Align 305129 32 21 ; Align 462631 40 6 ; Align 570064 42 33 ; Align 616128 54 81 ; Align 656157 82 33 ; Align 756843 93 30 ; Align 757092 104 33 ; Align 862920 115 93 ; Align 954545 146 9 ; Align 987687 149 42 ; Align 1139181 163 3 ; Align 1156248 165 12 ; Align 1162287 170 42 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 113 Query range: 4 -> 195 Target range: 12093 -> 1123066 5 : ArgLeuCysArgLeuLeuLysProAlaLeu{Le} >>>> Target Intron 1 : 15 ||| ! !||||||! !:!!|||||| !{ } 33417 bp ArgAlaProArgLeuTrpGlnProAlaLys{Se}++ 12094 : AGGGCCCCCCGCCTCTGGCAACCAGCAAAA{AG}gt.................... : 12128 16 : >>>> {u}Cys{G} >>>> Target Intron 2 >>>> {ly}AlaLeuA : 20 {!}|||{|} 76573 bp {||} ! !! ++{r}Cys{G}++ ++{ly}LeuArgA 12129 : .....ag{t}tgt{g}gt.........................ag{gg}CTGAGGG : 122129 21 : laAlaProGlyLeuAlaGlyThrMetCys{Al} >>>> Target Intron 3 : 30 !||||||||| ...||||||:!!...{ } 68654 bp spAlaProGlyGlyGlyGlyThrLeuAla{Il}++ 122130 : ATGctcctgggggtggagggacaCTGGCA{AT}gt..................... : 122163 31 : >>>> {a}SerArgAspAspTrpArgCysAlaArg{Se} >>>> Target In : 40 {!}|||!:!! !..!|||||||||::!|||{!!} 1202 ++{e}SerLysGlySerTrpArgCysSerArg{Ar}+- 122164 : ....ag{A}TCTAAGGGCTCCTGGCGCTGTAGCCGG{AG}gc.............. : 190847 41 : tron 4 >>>> {r}MetHisGluPheSerAlaLysAspIleAspGlyHisMetV : 54 bp {!}...|||! ! !:!!||| ! ||| !! !|||:!!! | ++{g}PheHisAlaGlyAlaAlaAspAspGlyGlyGlyTyrThrV 190848 : ...........ag{g}ttCCATGCGGGCGCGGCGGACGACGGCGGCGGCTACACCG : 192087 55 : alAsn >>>> Target Intron 5 >>>> LeuAspLysTyrArgGlyPheV : 63 ||.!. 132396 bp :!!||| !! !||||||: alGlu++ ++ValAspGlyAlaLeuGlyPheL 192088 : TAGAGgt.........................agGTGGATGGTGCCCTCGGCTTCC : 324510 64 : al >>>> Target Intron 6 >>>> CysIleValThrAsnValAlaSerG : 72 !! 50460 bp ||| ... ...|||. eu++ ++GlyIlePheProThrGlnGlySerA 324511 : TGgt.........................aggggatctttccaacccagggatcga : 374997 73 : lnUnk{G} >>>> Target Intron 7 >>>> {ly}{Ly} >>>> Targ : 75 .....{|} 23300 bp {||}{||} snSer{G}++ ++{ly}{Ly}++ 374998 : attca{g}gt.........................ag{ga}{aa}gt......... : 398310 76 : et Intron 8 >>>> {s}ThrGluValAsnTyrThrGlnLeuValAspLeu{H : 87 6522 bp {|}:!!|||..!::! ! !...:!!..! !|||{| ++{s}SerGluThrSerLeuLeuSerIleThrProLeu{H 398311 : ................ag{A}TCAGAGACCTCTCTCCTCTCCATCACCCCCCTA{C : 404864 88 : } >>>> Target Intron 9 >>>> {is}AlaArgTyrAlaGluCys >> : 94 } 6132 bp {||} :::|||... ||| }++ ++{is}MetGlnTyrGlyValCys++ 404865 : }gt.........................ag{AC}ATgcaatatggtgtgtgtgt.. : 411019 95 : >> Target Intron 10 >>>> GlyLeuArgIleLeu >>>> Target I : 99 9422 bp |||||||||.!! ! 9871 ++GlyLeuArgPheGlu++ 411020 : ........................agGGCCTCAGGTTCGAGgt............. : 420456 100 : ntron 11 >>>> Ala{P} >>>> Target Intron 12 >>>> {he}P : 101 3 bp |||{!} 42248 bp {::} ++Ala{T}++ -+{rp}T 420457 : .............agGCA{T}gt..........................tg{GG}A : 561421 102 : roCysAsnGlnPheGlyLysGlnGluPro{Gl} >>>> Target Intron 13 : 111 !!|||.!.!::||| !!:!!.!.:!! !!{||} 1002 bp hrCysGluArgPheArgGluAsnGlnThr{Gl}++ 561422 : CATGCGAACGCTTCAGGGAAAACCAAACC{GG}gt..................... : 561455 112 : >>>> {y}SerAsn{G} >>>> Target Intron 14 >>>> {lu}Glu : 115 {|}!:!!..{!} 39015 bp { }||| ++{y}AsnArg{G}++ ++{ly}Glu 561456 : .....ag{T}AACAGA{G}gt..........................ag{gt}gag : 601480 116 : IleLysGluPheAlaAlaGlyTyrAsnValLys >>>> Target Intron 15 : 127 :::.........|||...|||:::::::::::: 65142 bp ValSerArgIleAlaGlyGlyPheSerLeuGln-+ 601481 : gtctcccgcattgcaggcggattctcattgcagct..................... : 601518 128 : >>>> >>>> Target Intron 16 >>>> PheAspMetPheSer{L} : 132 30060 bp ||| ...:::{!} ++++ ++AlaAspSerLeuAla{A}+ 601519 : .....aggt..........................aggcggattctttagca{a}g : 696734 133 : >>>> Target Intron 17 >>>> {ys}IleCysValAsn{G} >>>> T : 137 87267 bp {!.}:::||| ...{!} + ++{sn}LeuCysGlnGln{A}++ 696735 : t..........................ag{ac}ctttgtcagcaa{g}gt...... : 784018 138 : arget Intron 18 >>>> {ly}AspAspAlaHisProLeuTrpLysTrpMet : 147 88450 bp {.!} ... ||||||||| ! ++{la}GlyArgGlyTrpGlyLeuTrpLysHisTrp 784019 : ....................ag{ca}gggcggggctgggggctgtgGAAACACTGG : 872495 148 : LysIleGlnProLysGlyLysGlyIleLeuGlyAsnAlaIle >>>> Target : 162 !:!:!! ! |||:!!!.!:!!||| !|||..! .!!||| 181 ArgValTyrProGlnAlaGlnGlyProLeuSerValThrIle++ 872496 : AGGGTTTACCCCCAGGCCCAGGGGCCCCTCTCTGTGACCATCgt............ : 872542 163 : Intron 19 >>>> Lys{T} >>>> Target Intron 20 >>>> {rp} : 163 84 bp |||{|} 40431 bp {||} ++Lys{T}++ ++{rp} 872543 : ..............agaaa{t}gt..........................ag{GG} : 931159 164 : Asn{P} >>>> Target Intron 21 >>>> {he}ThrLysPheLeuIleA : 171 :!!{ } 61261 bp { } |||... ... His{G}++ ++{ly}IleLysLeuThrPheA 931160 : CAT{G}gt..........................ag{gg}atcaaactcacatttg : 992443 172 : spLysAsnGlyCysValVal{Ly} >>>> Target Intron 22 >>>> {s : 178 ! !::: !|||:!:...{!:} 62861 bp {! laValSerProCysIleAla{Ar}++ ++{g 992444 : CTGTGTCACCTTGCATtgcc{ag}gt..........................ag{G : 1055327 179 : }Arg{T} >>>> Target Intron 23 >>>> {yr}GlyProMetGluGlu : 185 }!:!{ } 19177 bp { }|||||| ! !! ! }Lys{A}++ ++{la}GlyProGluTrpAla 1055328 : }AAG{G}gt..........................ag{CA}GGTCCAGAGTGGGCA : 1074523 186 : ProLeuValIleGluLys{As} >>>> Target Intron 24 >>>> {p}L : 193 !|||:!! !|||!:!{! } 48511 bp { }| AspLeuLeuGlyGluArg{Gl}++ ++{y}L 1074524 : GATCTGCTGGGAGAGAGG{GG}gt..........................ag{g}c : 1123058 194 : euProHis : 195 |||||||| euProHis 1123059 : tgccgcat : 1123066 vulgar: SPP00000007_1.0 4 195 . NIVO01030317.1:subseq(555646,1182752) 12093 1123066 + 113 M 10 30 S 0 2 5 0 2 I 0 33413 3 0 2 S 1 1 M 1 3 S 0 1 5 0 2 I 0 76569 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 68650 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 1198 3 0 2 S 1 1 M 15 45 5 0 2 I 0 132392 3 0 2 M 8 24 5 0 2 I 0 50456 3 0 2 M 10 30 S 0 1 5 0 2 I 0 23296 3 0 2 S 1 4 5 0 2 I 0 6518 3 0 2 S 1 1 M 11 33 S 0 1 5 0 2 I 0 6128 3 0 2 S 1 2 M 6 18 5 0 2 I 0 9418 3 0 2 M 5 15 5 0 2 I 0 98709 3 0 2 M 1 3 S 0 1 5 0 2 I 0 42244 3 0 2 S 1 2 M 10 30 S 0 2 5 0 2 I 0 998 3 0 2 S 1 1 M 2 6 S 0 1 5 0 2 I 0 39011 3 0 2 S 1 2 M 12 36 5 0 2 I 0 65138 3 0 2 5 0 2 I 0 30056 3 0 2 M 5 15 S 0 1 5 0 2 I 0 87263 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 88446 3 0 2 S 1 2 M 24 72 5 0 2 I 0 18180 3 0 2 M 1 3 S 0 1 5 0 2 I 0 40427 3 0 2 S 1 2 M 1 3 S 0 1 5 0 2 I 0 61257 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 62857 3 0 2 S 1 1 M 1 3 S 0 1 5 0 2 I 0 19173 3 0 2 S 1 2 M 11 33 S 0 2 5 0 2 I 0 48507 3 0 2 S 1 1 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 12094 1123066 113 + . gene_id 24 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 12094 12125 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 12094 12125 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 12126 12127 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 12126 45542 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 45541 45542 . + . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 45543 45547 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 45543 45547 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 45548 45549 . + . intron_id 2 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 45548 122120 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 122119 122120 . + . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 122121 122160 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 122121 122160 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 122161 122162 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 122161 190814 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 190813 190814 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 190815 190844 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 190815 190844 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 190845 190846 . + . intron_id 4 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 190845 192046 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 192045 192046 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 192047 192092 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 192047 192092 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 192093 192094 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 192093 324488 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 324487 324488 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 324489 324512 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 324489 324512 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 324513 324514 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 324513 374972 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 374971 374972 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 374973 375003 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 374973 375003 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 375004 375005 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 375004 398303 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 398302 398303 . + . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 398304 398307 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 398304 398307 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 398308 398309 . + . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 398308 404829 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 404828 404829 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 404830 404864 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 404830 404864 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 404865 404866 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 404865 410996 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 410995 410996 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 410997 411016 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 410997 411016 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 411017 411018 . + . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 411017 420438 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 420437 420438 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 420439 420453 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 420439 420453 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 420454 420455 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 420454 519166 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 519165 519166 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 519167 519170 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 519167 519170 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 519171 519172 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 519171 561418 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 561417 561418 . + . intron_id 11 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 561419 561452 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 561419 561452 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 561453 561454 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 561453 562454 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 562453 562454 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 562455 562462 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 562455 562462 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 562463 562464 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 562463 601477 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601476 601477 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601478 601515 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601478 601515 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601516 601517 . + . intron_id 15 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601516 666657 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 696717 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 696716 696717 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 696718 696733 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 696718 696733 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 696734 696735 . + . intron_id 17 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 696734 784000 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 783999 784000 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 784001 784015 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 784001 784015 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 784016 784017 . + . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 784016 872465 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 872464 872465 . + . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 872466 872539 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 872466 872539 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 872540 872541 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 872540 890723 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 890722 890723 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 890724 890727 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 890724 890727 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 890728 890729 . + . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 890728 931158 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 931157 931158 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 931159 931164 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 931159 931164 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 931165 931166 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 931165 992425 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 992424 992425 . + . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 992426 992465 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 992426 992465 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 992466 992467 . + . intron_id 22 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 992466 1055326 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1055325 1055326 . + . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1055327 1055331 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1055327 1055331 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1055332 1055333 . + . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1055332 1074508 . + . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1074507 1074508 . + . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1074509 1074545 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1074509 1074545 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1074546 1074547 . + . intron_id 24 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1074546 1123056 . + . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1123055 1123056 . + . intron_id 23 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1123057 1123066 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1123057 1123066 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 12094 1123066 113 + . alignment_id 24 ; Query SPP00000007_1.0 ; Align 12094 5 30 ; Align 45544 16 3 ; Align 122123 18 36 ; Align 190816 31 27 ; Align 192048 41 45 ; Align 324489 56 24 ; Align 374973 64 30 ; Align 404831 76 33 ; Align 410999 88 18 ; Align 420439 94 15 ; Align 519167 99 3 ; Align 561421 101 30 ; Align 562456 112 6 ; Align 601480 115 36 ; Align 696718 127 15 ; Align 784003 133 12 ; Align 872468 138 72 ; Align 890724 162 3 ; Align 931161 164 3 ; Align 992428 166 36 ; Align 1055328 179 3 ; Align 1074511 181 33 ; Align 1123058 193 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 106 Query range: 3 -> 195 Target range: 172477 -> 942968 4 : GlyArgLeuCys{Ar} >>>> Target Intron 1 >>>> {g}LeuLeuLysP : 12 ||||||:!!|||{||} 35202 bp {|} ...| GlyArgMetCys{Ar}++ ++{g}GlySerSerP 172478 : GGCAGGATGTGC{AG}gt.........................ag{g}ggatcttccc : 207704 13 : roAlaLeuLeuCysGlyAlaLeuAla{A} >>>> Target Intron 2 >>>> : 21 || ||||||||| |||...{.} 59545 bp roHisLeuLeuCysLeuLeuLeuCys{T}++ ++ 207705 : cacatctcctgtgtcttctgctctgc{a}gt.........................ag : 267275 22 : {la}ProGlyLeuAlaGlyThrMetCysAlaSer{Ar} >>>> Target Intron : 32 {!!}||| !!||| ! ! !!!!: !||| !{||} 2896 bp {hr}ProArgLeuPheHisProIleLeuAlaVal{Ar}++ 267276 : {CT}CCCCGCCTGTTCCACCCGATCCTTGCTGTG{CG}gt.................. : 267313 33 : 3 >>>> {g}AspAspTrpArgCysAlaArgSerMetHisGluPheSerAlaLys{ : 48 {|}..! |||||| !!!! !! ..!!:! !! ! !{ ++{g}SerThrThrArgCysGlnSerProAlaMetSerTyrProTrpGly{ 267314 : .......ag{G}TCTACGACCAGATGCCAGAGCCCCGCTATGTCGTATCCATGGGGA{ : 270253 49 : A} >>>> Target Intron 4 >>>> {sp}IleAspGlyHisMetVal{A} : 55 |} 7814 bp {||}:!:|||..!.!.! ! !{:} A}++ ++{sp}LeuAspSerGluLysGlu{A}++ 270254 : G}gt.........................ag{AC}TTGGACTCAGAGAAAGAA{G}gt : 278089 56 : >>>> Target Intron 5 >>>> {sn}LeuAspLysTyrArgGlyPheValCys : 64 13652 bp {!!} ! ! !!:!!.! !! ||| ++{sp}GlyLysLeuPheHisThr***ValVal 278090 : .........................ag{AT}GGCAAACTATTTCACACTTAGGTGGTG : 291767 65 : IleValThrAsnValAlaSerGlnUnk{G} >>>> Target Intron 6 >>>> : 74 :!!|||.!!!:!:!!|||||| ! {|} 30655 bp ValValAlaSerLeuAlaSerAlaGln{G}++ + 291768 : GTTGTAGCGAGCCTGGCCTCAGCACAG{G}gt.........................a : 322451 75 : {ly}LysThrGluValAsn{Ty} >>>> Target Intron 7 >>>> {r}Th : 81 {||} !!:!!!:!.!::!{ } 74943 bp {!} +{ly}LeuSerAspAlaSer{Gl}++ ++{y}Ar 322452 : g{GG}CTGAGTGATGCTTCC{GG}gt.........................ag{c}ag : 397416 82 : rGlnLeuValAspLeuHisAlaArgTyrAlaGlu >>>> Target Intron 8 > : 93 ...|||:::|||||||||...:::|||:::.!. 137985 bp gSerLeuLeuAspLeuHisValLysTyrSerHis++ 397417 : gtccttgctggatctccatgttaaatacagccATgt...................... : 397454 94 : >>> CysGlyLeuArgIleLeuAlaPheProCysAsnGlnPheGlyLysGlnGluPr : 110 ||| !||| !.!!:!!|||.!. !|||::!||| !||| ||| || -+CysPheLeuValPheValAlaLeuValCysSerGlnThrGlyCysGlnLeuPr 397455 : ...tgTGCTTTCTCGTCTTCGTCGCCCTGGTGTGTTCCCAGACCGGCTGCCAGCTCCC : 535488 111 : oGly{Se} >>>> Target Intron 9 >>>> {r}AsnGlu{G} >>>> Ta : 115 |!.!{.!} 26959 bp {!}|||..!{|} oAla{Gl}++ ++{y}AsnArg{G}++ 535489 : AGCA{GG}gt.........................ag{T}AACAGA{G}gt....... : 562465 116 : rget Intron 10 >>>> {lu}IleLysGluPheAla{Al} >>>> Target : 121 29662 bp {||}||||||:!!! |||{ } 10 -+{lu}IleLysGlnSerAla{Ar}++ 562466 : ...................tg{AA}ATCAAGCAATCAGCA{AG}gt............ : 592146 122 : Intron 11 >>>> {a}GlyTyrAsnValLysPheAspMetPheSerLysIleCys : 134 43 bp {!}||| !!.! !|||... ... |||||| ++{g}GlyProProAlaProPheSerArgIleProTyrIleCys 592147 : ..............ag{A}GGTCCACCTGCACCGTTCTCcaggattccatatatatgc : 593224 135 : ValAsn{Gl} >>>> Target Intron 12 >>>> {y}AspAspAlaHisPro : 142 ||||||{ } 19161 bp {!}||||||!.!! !||| ValAsn{Me}++ ++{t}AspAspGlyLeuPro 593225 : gttaat{at}gt..........................ag{G}GATGACGGGCTCCCC : 612409 143 : LeuTrpLysTrpMetLys >>>> Target Intron 13 >>>> IleGlnProL : 152 ||| ! ::: !!.!. ! 47661 bp |||| LeuGlyArgGlyPheHis++ ++LysThrProL 612410 : TTAGGCCGTGGGTTCCATgt..........................agaaaaccccaa : 660100 153 : ysGlyLysGlyIleLeuGlyAsnAlaIleLysTrpAsnPheThrLysPheLeuIleAs : 171 || ::: ... |||.!! !!! ! !||| ! !!::|||:!!! ysArgMetTyrValCysAlaLeuAlaPheLeuCysIlePheTrpValTrpLeuValGl 660101 : agaggatgtatgtgtgtgcacttgCATTCCTGTGTATCTTTTGGGTGTGGTTGGTAGG : 660157 172 : pLysAsnGlyCysValValLys{A} >>>> Target Intron 14 >>>> {rg : 179 ......||||||! !:!!|||{ } 38262 bp { ! ySerGlyGlyCysAspIleLys{S}++ ++{er 660158 : GTCTGGGGGCTGTGATATAAAA{T}gt..........................ag{ct : 698444 180 : }TyrGlyProMet{Gl} >>>> Target Intron 15 >>>> {u}GluProLe : 187 }::: ||| {..} 141090 bp {!}||||||! }PhePheProGlu{Ar}+- ++{g}GluProGl 698445 : }ttttttccagaa{ag}ga..........................ag{A}GAACCCCA : 839557 188 : uValIleGlu{Ly} >>>> Target Intron 16 >>>> {s}AspLeuProHi : 195 !! !|||...{!:} 103385 bp {!}||||||||||| nGluIleSer{Ar}++ ++{g}AspLeuProHi 839558 : AGAGATCTCC{AG}gt..........................ag{g}gatcttcccca : 942966 196 : s : 195 | s 942967 : c : 942968 vulgar: SPP00000007_1.0 3 195 . NIVO01030317.1:subseq(555646,1182752) 172477 942968 + 106 M 4 12 S 0 2 5 0 2 I 0 35198 3 0 2 S 1 1 M 12 36 S 0 1 5 0 2 I 0 59541 3 0 2 S 1 2 M 10 30 S 0 2 5 0 2 I 0 2892 3 0 2 S 1 1 M 15 45 S 0 1 5 0 2 I 0 7810 3 0 2 S 1 2 M 6 18 S 0 1 5 0 2 I 0 13648 3 0 2 S 1 2 M 18 54 S 0 1 5 0 2 I 0 30651 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 74939 3 0 2 S 1 1 M 12 36 5 0 2 I 0 137981 3 0 2 M 19 57 S 0 2 5 0 2 I 0 26955 3 0 2 S 1 1 M 2 6 S 0 1 5 0 2 I 0 29658 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 1039 3 0 2 S 1 1 M 15 45 S 0 2 5 0 2 I 0 19157 3 0 2 S 1 1 M 11 33 5 0 2 I 0 47657 3 0 2 M 30 90 S 0 1 5 0 2 I 0 38258 3 0 2 S 1 2 M 4 12 S 0 2 5 0 2 I 0 141086 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 103381 3 0 2 S 1 1 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 172478 942968 106 + . gene_id 25 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 172478 172491 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 172478 172491 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 172492 172493 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 172492 207693 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 207692 207693 . + . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 207694 207731 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 207694 207731 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 207732 207733 . + . intron_id 2 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 207732 267276 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 267275 267276 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 267277 267310 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 267277 267310 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 267311 267312 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 267311 270206 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 270205 270206 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 270207 270253 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 270207 270253 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 270254 270255 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 270254 278067 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 278066 278067 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 278068 278088 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 278068 278088 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 278089 278090 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 278089 291740 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 291739 291740 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 291741 291797 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 291741 291797 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 291798 291799 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 291798 322452 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 322451 322452 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 322453 322471 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 322453 322471 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 322472 322473 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 322472 397414 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 397413 397414 . + . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 397415 397451 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 397415 397451 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 397452 397453 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 397452 535436 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 535435 535436 . + . intron_id 7 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 535437 535495 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 535437 535495 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 535496 535497 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 535496 562454 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 562453 562454 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 562455 562462 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 562455 562462 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 562463 562464 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 562463 592124 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 592123 592124 . + . intron_id 9 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 592125 592143 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 592125 592143 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 592144 592145 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 592144 593186 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 593185 593186 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 593187 593234 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 593187 593234 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 593235 593236 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 593235 612395 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 612394 612395 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 612396 612429 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 612396 612429 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 612430 612431 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 612430 660090 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 660089 660090 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 660091 660181 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 660091 660181 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 660182 660183 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 660182 698443 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 698442 698443 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 698444 698459 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 698444 698459 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 698460 698461 . + . intron_id 15 ; splice_site "ga" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 698460 839549 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 839548 839549 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 839550 839570 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 839550 839570 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 839571 839572 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 839571 942955 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 942954 942955 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 942956 942968 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 942956 942968 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 172478 942968 106 + . alignment_id 25 ; Query SPP00000007_1.0 ; Align 172478 4 12 ; Align 207695 9 36 ; Align 267279 22 30 ; Align 270208 33 45 ; Align 278070 49 18 ; Align 291743 56 54 ; Align 322455 75 15 ; Align 397416 81 36 ; Align 535437 93 57 ; Align 562456 113 6 ; Align 592127 116 15 ; Align 593188 122 45 ; Align 612397 138 33 ; Align 660091 149 90 ; Align 698446 180 12 ; Align 839551 185 18 ; Align 942957 192 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 111 Query range: 1 -> 196 Target range: 182567 -> 1162446 2 : SerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCys >>>> Targ : 17 ..!||||||! !||| !!|||||||||:!!||| !! !! ! GlnLeuGlyProLeuArgArgLeuLeuGluProGlnGlnProGln++ 182568 : CAACTCGGACCGCTACGCCGTCTACTGGAACCGCAGCAACCCCAGgt......... : 182615 18 : et Intron 1 >>>> GlyAlaLeuAlaAlaProGlyLeu{Al} >>>> Tar : 25 40185 bp ||| ! !!.!::!|||||||||{!.} ++GlyIleSerValSerProGlyLeu{Gl}++ 182616 : ................agGGAATTAGTGTCAGCCCCGGGCTG{GG}gt........ : 222826 26 : get Intron 2 >>>> {a} >>>> Target Intron 3 >>>> GlyTh : 27 33448 bp {!} 31049 bp ||| ++{y}++ ++GlyGl 222827 : .................ag{G}gt.........................agGGAGA : 287325 28 : rMetCysAlaSerArgAspAspTrpArgCysAlaArgSerMetHis{Gl} >>>> : 43 !! |||::!.!!|||||| !!! ! !||| !!:!!! !{..} uSerCysSerGlyArgAspPro***AlaGlnLeuArgProLeuPro{Ar}++ 287326 : GAGCTGCAGTGGGAGGGACCCCTGAGCACAGCTGAGGCCACTGCCT{AG}gt.... : 287377 44 : Target Intron 4 >>>> {u}PheSerAlaLysAspIleAspGlyHisMet : 53 3390 bp {!} ||| !|||||| !! !.! ! -+{g}AsnSerArgLysAspProGlyAlaIleThr 287378 : .....................tg{a}aatAGCAGAAAGGACCCAGGAGCGATAACA : 290793 54 : ValAsnLeuAspLysTyrArgGlyPheValCysIleValThrAsnValAlaSerGl : 72 ||||||! ! !:!||| !..! ! !! !:!!..!! ! ||| !..!.. ValAsnGlnIleArgTyrLeuSerGluGluPheLeuThrIleValValArgGlnSe 290794 : GTAAATCAAATAAGATATTTGTCGGAGGAATTCCTCACAATTGTGGTGAGACAGAG : 290850 73 : nUnkGlyLysThrGluValAsnTyrThr{Gl} >>>> Target Intron 5 > : 82 ....|||!!.|||..! !|||! !{ } 117818 bp rSerGlyAsnThrSerArgAsnLeuGlu{**}++ 290851 : CTCAGGGAATACTTCAAGAAATTTGGAG{TG}gt...................... : 290884 83 : >>> {n}LeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArgIleLeu : 98 {!} :::||| :::..!!:!|||!.!! ||| ::: ||| +-{*}TyrIleAspThrTyrValGlnTyrGlyValCysHisIleTyrIleTyr 290885 : ...ac{a}tatatagatacatacgTGCAATATGGTGTgtgtcacatatatatatac : 408746 99 : AlaPheProCysAsnGln >>>> Target Intron 6 >>>> PheGlyLys : 107 ||| |||... 21621 bp .!! !!!:! IlePheThrTyrAsnMet++ ++LeuArgArg 408747 : atatttacatacaatatggt.........................agCTCCGCAGG : 430394 108 : GlnGluProGlySer{As} >>>> Target Intron 7 >>>> {n}GluGl : 115 |||!!:|||!.!:!!{||} 47831 bp {|}..!.. GlnAspProAlaAla{As}++ ++{n}ArgSe 430395 : CAGGACCCGGCCGCC{AA}gt.........................ag{C}CGGAG : 478249 116 : uIleLysGluPheAlaAlaGlyTyrAsnValLysPheAspMetPheSerLys{Il} : 133 .:!:!:!|||.!...! !!||| !!... ! .!.|||:!! !!.!!! { } rValArgGluLeuCysProGlyAspGlySerLeuLeuAspLeuValGlyThr{Hi} 478250 : CGTGAGAGAGCTGTGCCCAGGAGACGGGTCCCTCCTGGACCTGGTTGGAACC{CA} : 478303 134 : >>>> Target Intron 8 >>>> {e}CysValAsn >>>> Target I : 137 24428 bp {!}||||||||| 8183 ++ ++{s}CysValAsn++ 478304 : gt.........................ag{C}TGTGTGAATgt............. : 502745 138 : ntron 9 >>>> GlyAspAspAlaHisProLeuTrpLysTrpMet >>>> Ta : 148 1 bp |||...:::|||||| ||| :::|||... ++GlySerAsnAlaHis---LeuLeuGlnTrpGln++ 502746 : ............agggatcgaacgctcat---ctcttgcaatggcaggt....... : 584606 149 : rget Intron 10 >>>> LysIleGlnProLysGly >>>> Target Int : 154 16856 bp ||| ||| 65180 ++GlyIlePheProIleGln++ 584607 : ...................aggggatcttcccaatccaggt............... : 601480 155 : ron 11 >>>> >>>> Target Intron 12 >>>> LysGlyIleLeuG : 158 bp 8957 bp |||! !:!!|||| ++++ ++LysGluValLeuG 601481 : ...........aggt..........................agAAAGAAGTACTGG : 675627 159 : lyAsnAla{Il} >>>> Target Intron 13 >>>> {e}LysTrpAsnPh : 165 ||!..!.!{:!} 110554 bp {!} ::::::.. lyArgVal{Le}++ ++{u}ThrPheSerLe 675628 : GCAGGGTT{TT}gt..........................ag{a}actttctccct : 786202 166 : eThrLysPheLeuIleAsp{L} >>>> Target Intron 14 >>>> {ys} : 172 . |||...:::...{:} 43296 bp {!!} uPhePhePhePheLeuGln{G}++ ++{lu} 786203 : tttttttttttttcttcaa{g}gt..........................ag{AG} : 829520 173 : AsnGlyCysValValLysArgTyrGlyProMetGluGluProLeuVal >>>> T : 189 !.!||||||..!!..! !!::|||||| ! ! ||| ! ! ProAlaCysValThrSerThrTrpGlyProSerMetProProAlaLys++ 829521 : CCAGCTTGCGTGACCAGCACTTGGGGACCCTCGATGCCCCCTGCTAAGgt...... : 829572 190 : arget Intron 15 >>>> IleGluLysAsp{Le} >>>> Target Intr : 193 181807 bp :!:..!|||..!{||} 151046 ++ValArgLysSer{Le}++ 829573 : ....................agGTGAGGAAGTCC{CT}gt................ : 1011393 194 : on 16 >>>> {u}ProHisTyr : 196 bp {|}||||||:!! ++{u}ProHisHis 1011394 : ..........ag{G}CCCCACCAT : 1162446 vulgar: SPP00000007_1.0 1 196 . NIVO01030317.1:subseq(555646,1182752) 182567 1162446 + 111 M 15 45 5 0 2 I 0 40181 3 0 2 M 8 24 S 0 2 5 0 2 I 0 33444 3 0 2 S 1 1 5 0 2 I 0 31045 3 0 2 M 17 51 S 0 2 5 0 2 I 0 3386 3 0 2 S 1 1 M 38 114 S 0 2 5 0 2 I 0 117814 3 0 2 S 1 1 M 22 66 5 0 2 I 0 21617 3 0 2 M 8 24 S 0 2 5 0 2 I 0 47827 3 0 2 S 1 1 M 19 57 S 0 2 5 0 2 I 0 24424 3 0 2 S 1 1 M 3 9 5 0 2 I 0 81827 3 0 2 M 5 15 G 1 0 M 5 15 5 0 2 I 0 16852 3 0 2 M 6 18 5 0 2 I 0 65176 3 0 2 5 0 2 I 0 8953 3 0 2 M 7 21 S 0 2 5 0 2 I 0 110550 3 0 2 S 1 1 M 10 30 S 0 1 5 0 2 I 0 43292 3 0 2 S 1 2 M 16 48 5 0 2 I 0 181803 3 0 2 M 4 12 S 0 2 5 0 2 I 0 151042 3 0 2 S 1 1 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 182568 1162446 111 + . gene_id 26 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 182568 182612 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 182568 182612 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 182613 182614 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 182613 222797 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 222796 222797 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 222798 222823 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 222798 222823 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 222824 222825 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 222824 256271 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 256270 256271 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 256272 256272 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 256272 256272 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 256273 256274 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 256273 287321 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 287320 287321 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 287322 287374 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 287322 287374 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 287375 287376 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 287375 290764 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 290763 290764 . + . intron_id 3 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 290765 290881 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 290765 290881 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 290882 290883 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 290882 408699 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 408698 408699 . + . intron_id 4 ; splice_site "ac" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 408700 408766 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 408700 408766 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 408767 408768 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 408767 430387 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 430386 430387 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 430388 430413 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 430388 430413 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 430414 430415 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 430414 478244 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 478243 478244 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 478245 478304 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 478245 478304 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 478305 478306 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 478305 502732 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 502731 502732 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 502733 502742 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 502733 502742 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 502743 502744 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 502743 584573 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 584572 584573 . + . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 584574 584603 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 584574 584603 . + . insertions 0 ; deletions 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 584604 584605 . + . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 584604 601459 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 666657 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 675614 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 675613 675614 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 675615 675637 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 675615 675637 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 675638 675639 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 675638 786191 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 786190 786191 . + . intron_id 12 ; splice_site "Ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 786192 786223 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 786192 786223 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 786224 786225 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 786224 829519 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 829518 829519 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 829520 829569 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 829520 829569 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 829570 829571 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 829570 1011376 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1011377 1011390 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1011377 1011390 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011391 1011392 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011391 1162436 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1162435 1162436 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1162437 1162446 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1162437 1162446 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 182568 1162446 111 + . alignment_id 26 ; Query SPP00000007_1.0 ; Align 182568 2 45 ; Align 222798 17 24 ; Align 287322 26 51 ; Align 290766 44 114 ; Align 408701 83 66 ; Align 430388 105 24 ; Align 478246 114 57 ; Align 502734 134 9 ; Align 584574 137 15 ; Align 584589 143 15 ; Align 601460 148 18 ; Align 675615 154 21 ; Align 786193 162 30 ; Align 829522 173 48 ; Align 1011377 189 12 ; Align 1162438 194 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 111 Query range: 7 -> 194 Target range: 262740 -> 1162478 8 : ArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeu{A} >>>> Target Int : 20 ||| !|||:!!|||||| !||| !|||:!!! !{|} 73046 ArgLysLeuGluProAlaAspLeuThrGlySerPro{A}++ 262741 : AGGAAGCTGGAGCCAGCTGACCTAACTGGCTCACCA{G}gt............... : 262780 21 : ron 1 >>>> {la}AlaPro >>>> Target Intron 2 >>>> GlyLe : 24 bp {||}|||||| 57446 bp |||:: ++{la}AlaPro++ ++GlyIl 262781 : ..........ag{cc}gCCCCTgt.........................aggggat : 393281 25 : uAlaGlyThrMetCysAlaSerArgAspAspTrpArgCysAlaArgSerMetHisG : 43 : |||... ::: |||...||| ||| ::::::: ePheProThrGlnGlyLeuAsnProGlyPheTrpHisCysArgArgIleLeuTyrG 393282 : tttcccgacccagggattgaacccaggtttttggcattgcaggcggattctttacc : 393338 44 : luPheSerAlaLys >>>> Target Intron 3 >>>> AspIleAsp{G} : 51 ::...||| ::: 12748 bp ||| {|} lnLeuSerArgGln+- ++AspSerPro{G}+ 393339 : agctgagccgccagga.........................aggattctcca{g}g : 406111 52 : >>>> Target Intron 4 >>>> {ly}HisMetValAsn{L} >>>> Ta : 56 56519 bp {||} ||| ...{|} - ++{ly}ValMetTyrGly{L}++ 406112 : c.........................ag{ga}gtcatgtatgga{t}gt....... : 462647 57 : rget Intron 5 >>>> {eu}AspLysTyrArgGlyPheValCys >>>> T : 65 75561 bp {||}|||...:!!|||! !|||:!!! ! ++{eu}AspSerHisArgAspPheLeuTyr++ 462648 : ..................ag{tg}gattcTCATCGAGATTTTCTTTACgt...... : 538234 66 : arget Intron 6 >>>> IleValThrAsnValAlaSerGlnUnk >>>> T : 74 35868 bp :::... ::::::|||!!!..! ++ValAlaIleSerIleAlaThrMetGly++ 538235 : ...................aggttgccatttccatagCAACAATGGGAgt...... : 574129 75 : arget Intron 7 >>>> GlyLysThrGluValAsn >>>> Target Int : 80 27333 bp ||| :::... 54677 ++GlyIlePheProIleGln++ 574130 : ...................aggggatcttcccaatccaggt............... : 601480 81 : ron 8 >>>> TyrThrGlnLeuValAspLeuHisAlaArgTyr{Al} >>>> : 91 bp ! ! !:!!! !:!!||| !|||||| !! !{::} ++GluArgGluSerLeuAspThrHisAlaPheCys{Se}++ 601481 : ..........agGAAAGGGAGTCTCTAGACACTCACGCTTTCTGC{AG}gt..... : 656192 92 : Target Intron 9 >>>> {a}GluCysGlyLeuArgIle{Le} >>>> Ta : 98 159503 bp {!}||| ...|||||||||{ } ++{r}GluSerAlaLeuArgIle{Ar}++ 656193 : ....................ag{t}gagtcagctcttcgcatc{ag}gt....... : 815716 99 : rget Intron 10 >>>> {u}AlaPheProCysAsnGlnPheGlyLysGln{G : 109 47814 bp {!}...|||||| :::|||||| ...{| ++{g}ValPheProValSerGlnPhePheProSer{G 815717 : ...................ag{g}gtttttccagtgagtcagttcttcccatca{g : 863559 110 : } >>>> Target Intron 11 >>>> {lu}ProGlySerAsnGluGlu > : 116 } 27164 bp {||} ! !!||||||! ! }++ ++{lu}MetTyrProAsnGluGly++ 863560 : }gt..........................ag{aa}atgtACCCAAATGAAGGAgt. : 890746 117 : >>> Target Intron 12 >>>> IleLysGluPheAlaAlaGlyTyrAsnVa : 125 98453 bp |||......!:!..!..!||||||..! ++IleSerArgTyrCysCysGlyTyrGlySe 890747 : .........................agATTTCTCGTTATTGCTGTGGTTATGGTTC : 989224 126 : lLysPhe{As} >>>> Target Intron 13 >>>> {p}MetPheSer{L} : 132 ! !!!.{ } 37499 bp { }:::||||||{|} rValLeu{Ar}++ ++{g}ValPheSer{L} 989225 : TGTGTTA{CG}gt..........................ag{g}gtcttttca{a} : 1026744 133 : >>>> Target Intron 14 >>>> {ys}IleCys{Va} >>>> Targe : 135 13628 bp {||}:::|||{:!} ++ ++{ys}ValCys{Il}++ 1026745 : gt..........................ag{aa}gtgtgc{at}gt.......... : 1040385 136 : t Intron 15 >>>> {l}AsnGlyAspAspAlaHisProLeuTrpLysTrpMe : 147 560 bp {!}...! !|||.!. !.!. ! ! :!! :! ++{e}GlyGluAspGlnArgGluAsnAlaAlaGluHisLe 1040386 : ................ag{C}GGGGAAGACCAAAGGGAAAATGCCGCAGAGCATTT : 1040977 148 : tLysIleGlnProLys{Gl} >>>> Target Intron 16 >>>> {y}Lys : 154 :...:!!!!.|||:!!{||} 48277 bp {|} uSerLeuHisProGln{Gl}++ -+{y}Val 1040978 : ATCTCTTCATCCACAG{GG}gt..........................tg{g}gtc : 1089275 155 : GlyIleLeuGlyAsnAlaIleLysTrpAsnPheThrLysPheLeuIleAspLysAs : 173 ||| ::: ||||||... ||| |||::: .. GlyLysIleProTrpLysArgLysTrpGlnSerThrProValLeuLeuProGlyLy 1089276 : gggaagatcccctggaaaaggaaatggcaatccactccagtactattgcctggaaa : 1089332 174 : nGlyCysValValLysArgTyr >>>> Target Intron 17 >>>> >> : 181 ....||| ::: 22792 bp sSerCysGlyGlnArgSerLeu++ ++++ 1089333 : atcctgtggacagaggagcctggt..........................aggt.. : 1112150 182 : >> Target Intron 18 >>>> GlyProMetGluGluProLeuValIleGlu : 190 50289 bp !.!||| ..! |||||| ! !! ! ++AlaProProSerThrProLeuHisProAla 1112151 : ........................agGCCCCACCATCGACCCCTCTCCATCCTGCA : 1162464 191 : LysAspLeuPro : 194 :!! |||||| Gln***LeuPro 1162465 : CAGTGACTGCCG : 1162478 vulgar: SPP00000007_1.0 7 194 . NIVO01030317.1:subseq(555646,1182752) 262740 1162478 + 111 M 12 36 S 0 1 5 0 2 I 0 73042 3 0 2 S 1 2 M 2 6 5 0 2 I 0 57442 3 0 2 M 25 75 5 0 2 I 0 12744 3 0 2 M 3 9 S 0 1 5 0 2 I 0 56515 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 75557 3 0 2 S 1 2 M 8 24 5 0 2 I 0 35864 3 0 2 M 9 27 5 0 2 I 0 27329 3 0 2 M 6 18 5 0 2 I 0 54673 3 0 2 M 11 33 S 0 2 5 0 2 I 0 159499 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 47810 3 0 2 S 1 1 M 10 30 S 0 1 5 0 2 I 0 27160 3 0 2 S 1 2 M 6 18 5 0 2 I 0 98449 3 0 2 M 12 36 S 0 2 5 0 2 I 0 37495 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 13624 3 0 2 S 1 2 M 2 6 S 0 2 5 0 2 I 0 556 3 0 2 S 1 1 M 17 51 S 0 2 5 0 2 I 0 48273 3 0 2 S 1 1 M 27 81 5 0 2 I 0 22788 3 0 2 5 0 2 I 0 50285 3 0 2 M 14 42 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 262741 1162478 111 + . gene_id 27 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 262741 262777 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 262741 262777 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 262778 262779 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 262778 335823 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 335822 335823 . + . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 335824 335831 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 335824 335831 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 335832 335833 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 335832 393277 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 393276 393277 . + . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 393278 393352 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 393278 393352 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 393353 393354 . + . intron_id 3 ; splice_site "ga" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 393353 406100 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 406099 406100 . + . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 406101 406110 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 406101 406110 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 406111 406112 . + . intron_id 4 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 406111 462629 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462644 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462644 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462645 462646 . + . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462645 538205 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 538204 538205 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 538206 538231 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 538206 538231 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 538232 538233 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 538232 574099 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 574098 574099 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 574100 574126 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 574100 574126 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 574127 574128 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 574127 601459 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 656154 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 656153 656154 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 656155 656189 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 656155 656189 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 656190 656191 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 656190 815692 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 815691 815692 . + . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 815693 815713 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 815693 815713 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 815714 815715 . + . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 815714 863527 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863526 863527 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863528 863559 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863528 863559 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863560 863561 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863560 890723 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 890722 890723 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 890724 890743 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 890724 890743 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 890744 890745 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 890744 989196 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 989195 989196 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 989197 989234 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 989197 989234 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 989235 989236 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 989235 1026733 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1026732 1026733 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1026734 1026744 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1026734 1026744 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1026745 1026746 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1026745 1040372 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1040371 1040372 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1040373 1040382 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1040373 1040382 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1040383 1040384 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1040383 1040942 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1040941 1040942 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1040943 1040996 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1040943 1040996 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1040997 1040998 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1040997 1089273 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1089272 1089273 . + . intron_id 15 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1089274 1089355 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1089274 1089355 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1089356 1089357 . + . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1089356 1112147 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1162436 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1162435 1162436 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1162437 1162478 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1162437 1162478 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 262741 1162478 111 + . alignment_id 27 ; Query SPP00000007_1.0 ; Align 262741 8 36 ; Align 335826 21 6 ; Align 393278 23 75 ; Align 406101 48 9 ; Align 462632 52 12 ; Align 538208 57 24 ; Align 574100 65 27 ; Align 601460 74 18 ; Align 656155 80 33 ; Align 815694 92 18 ; Align 863529 99 30 ; Align 890726 110 18 ; Align 989197 116 36 ; Align 1026735 129 9 ; Align 1040375 133 6 ; Align 1040944 136 51 ; Align 1089275 154 81 ; Align 1162437 181 42 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 107 Query range: 12 -> 194 Target range: 62074 -> 1168493 13 : AlaLeuLeuCysGlyAlaLeuAlaAlaPro{Gl} >>>> Target Intron 1 : 23 |||||| !||| !|||:!!!.! !|||{.!} 39692 bp AlaLeuGluCysProAlaValGlyArgPro{Se}++ 62075 : GCCCTTGAATGCCCAGCTGTGGGGAGGCCA{AG}gt.................... : 62109 24 : >>>> {y}LeuAlaGlyThrMetCysAlaSerArgAspAsp{T} >>>> Tar : 35 {!}:!! ! !|||||| |||!.!||||||.!.{|} ++{r}IleHisProThrMetGlnAlaLysArgAspGln{T}++ 62110 : .....ag{C}ATCCACCCTACCATGCAGGCCAAGAGGGATCAA{T}gt........ : 101836 36 : get Intron 2 >>>> {rp}ArgCysAlaArgSerMetHisGluPheSerAla : 46 58991 bp {||}!:! ! .!!||||||:!:!.. !|||!.! ++{rp}GlnArgThrArgSerLeuArgCysHisSerGly 101837 : .................ag{GG}CAGAGGACAAGGAGCCTCCGGTGTCACTCGGGA : 160857 47 : Lys >>>> Target Intron 3 >>>> AspIleAspGlyHisMetValAsn : 55 :!! 54623 bp :::::: |||:::::: Gln++ ++GlyLeuAsnLeuHisLeuLeuCys 160858 : CAGgt.........................agggactgaacctccatctcctgtgt : 215507 56 : LeuAspLysTyrArg >>>> Target Intron 4 >>>> GlyPheValCys : 64 ||| :::... 42916 bp !.!.!!:!!||| LeuLeuHisTrpHis++ ++AlaIleIleCys 215508 : ctcctgcattggcatgt.........................agGCCATCATCTGC : 258450 65 : IleValThrAsnValAlaSer >>>> Target Intron 5 >>>> GlnUnk : 73 :!!:!! |||! !:!! 10208 bp ||| AlaIleSerAlaValAspAla++ ++GlnPro 258451 : GCGATCTCAGCAGTAGATGCGgt.........................agCAGCCG : 268685 74 : GlyLys >>>> Target Intron 6 >>>> ThrGluValAsnTyrThrGln : 82 |||::! 50263 bp .!!! :!!!!. !! !||| GlyArg++ ++AlaAlaIleLysLeuIleGln 268686 : GGGCGAgt.........................agGCCGCTATTAAGCTTATCCAG : 318975 83 : >>>> Target Intron 7 >>>> LeuValAspLeuHisAla{Ar} >>> : 89 27226 bp :!!|||||| !|||.!!{||} ++ ++IleValAspArgHisThr{Ar}++ 318976 : gt.........................agATTGTAGACAGACACACA{AG}gt... : 346226 90 : > Target Intron 8 >>>> {g}TyrAlaGlu{Cy} >>>> Target In : 93 64773 bp {|}:!!||| {||} 88759 ++{g}HisAlaIle{Cy}++ 346227 : ......................ag{A}CATgcaata{tg}gt.............. : 411011 94 : tron 9 >>>> {s}GlyLeuArgIleLeu{Al} >>>> Target Intron : 99 bp {|}||| !!:!:!!|||{!.} 101674 bp ++{s}GlyGlyLysValLeu{Gl}++ 411012 : ...........ag{C}GGTGGGAAGGTACTC{GG}gt................... : 499788 100 : 10 >>>> {a}PheProCysAsnGln{Ph} >>>> Target Intron 11 > : 105 {!} ......{ } 8719 bp ++{y}GlySerSerGlnSer{Ar}++ 499789 : .......ag{g}ggatcttcccaatcc{ag}gt....................... : 601480 106 : >>> {e}GlyLysGlnGluProGlySerAsnGluGluIleLysGluPheAlaAla : 121 { }|||.........||| ...... :::|||... ...|||... ++{g}GlySerSerArgProArgAspGlnThrGlnIleSerCysLeuAlaGly 601481 : ...ag{g}ggatcttcccgacccagggatcaaacccagatctcctgccttgcaggc : 610243 122 : GlyTyrAsnVal{Ly} >>>> Target Intron 12 >>>> {s}PheAspM : 129 |||::: ...{!:} 43891 bp {!}...:!:: GlyPhePheThr{Ar}-+ ++{g}IleGluL 610244 : ggattctttacc{ag}ct..........................ag{a}attgAGT : 654158 130 : etPheSerLysIleCysValAsnGly >>>> Target Intron 13 >>>> : 138 !:|||:!! ! ! :!!::! !! 12473 bp euPheAlaLeuGlyLeuLeuSerArg++ ++ 654159 : TATTCGCCTTAGGGTTGTTATCCAGGgt..........................ag : 666656 139 : >>>> Target Intron 14 >>>> AspAspAlaHisProLeuTrpLysTr : 146 178703 bp ! !..!! !||||||||||||!!.!! ++ -+ValSerGluHisProLeuTrpAsnCy 666657 : gt..........................tgGTCTCCGAGCACCCGCTTTGGAACTG : 845385 147 : pMet{Ly} >>>> Target Intron 15 >>>> {s}IleGlnProLysGly : 153 :!!{ } 61533 bp {!}:!! !!:::||| sVal{Pr}++ ++{o}ValAlaAlaArgGly 845386 : TGTG{CC}gt..........................ag{G}GTCGCTGCGCGCGGC : 906939 154 : Lys{Gl} >>>> Target Intron 16 >>>> {y}IleLeuGlyAsn{Al} : 160 {||} 7209 bp {|}:!!|||||| !{!.} Pro{Gl}++ ++{y}LeuLeuGlyLeu{Va} 906940 : CCC{GG}gt..........................ag{A}CTCCTGGGGCTT{GT} : 914169 161 : >>>> Target Intron 17 >>>> {a}IleLysTrpAsnPheThrLysPh : 168 68136 bp {!}:!:!..||| ! ! !!:! ++ ++{l}ValSerTrpCysValCysArgGl 914170 : gt..........................ag{g}gTGAGCTGGTGCGTGTGCAGAGG : 982329 169 : eLeuIleAspLys >>>> Target Intron 18 >>>> >>>> Target : 173 !:!! ! !:! 29033 bp 91 yValHisLeuArg++ ++++ 982330 : TGTACACCTAAGGgt..........................aggt........... : 1011379 174 : Intron 19 >>>> AsnGlyCysValValLysArgTyrGlyProMetGlu{G} : 185 044 bp !|||...!.!||| ! !!! |||||| !|||{|} ++ProGlyAlaAlaValLeuGlySerGlyProArgGlu{G} 1011380 : ...............agCCTGGTGCGGCCGTGCTGGGTTCAGGGCCACGGGAA{G} : 1102457 186 : >>>> Target Intron 20 >>>> {lu}ProLeuValIle{Gl} >>>> : 190 9674 bp {||}|||||| !!:!!{..} ++ -+{lu}ProLeuPheLeu{Ar}++ 1102458 : gt..........................cg{AG}CCTCTGTTTCTC{AG}gt.... : 1112150 191 : Target Intron 21 >>>> {u}LysAspLeuPro : 194 56333 bp {!} !||||||||| ++{g}GlyAspLeuPro 1112151 : ......................ag{A}GGAGACCTCCCC : 1168493 vulgar: SPP00000007_1.0 12 194 . NIVO01030317.1:subseq(555646,1182752) 62074 1168493 + 107 M 10 30 S 0 2 5 0 2 I 0 39688 3 0 2 S 1 1 M 11 33 S 0 1 5 0 2 I 0 58987 3 0 2 S 1 2 M 12 36 5 0 2 I 0 54619 3 0 2 M 13 39 5 0 2 I 0 42912 3 0 2 M 11 33 5 0 2 I 0 10204 3 0 2 M 4 12 5 0 2 I 0 50259 3 0 2 M 7 21 5 0 2 I 0 27222 3 0 2 M 6 18 S 0 2 5 0 2 I 0 64769 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 88755 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 101670 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 8715 3 0 2 S 1 1 M 20 60 S 0 2 5 0 2 I 0 43887 3 0 2 S 1 1 M 11 33 5 0 2 I 0 12469 3 0 2 5 0 2 I 0 178699 3 0 2 M 10 30 S 0 2 5 0 2 I 0 61529 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 7205 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 68132 3 0 2 S 1 1 M 12 36 5 0 2 I 0 29029 3 0 2 5 0 2 I 0 91040 3 0 2 M 12 36 S 0 1 5 0 2 I 0 9670 3 0 2 S 1 2 M 4 12 S 0 2 5 0 2 I 0 56329 3 0 2 S 1 1 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 62075 1168493 107 + . gene_id 28 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 62075 62106 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 62075 62106 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 62107 62108 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 62107 101798 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 101797 101798 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 101799 101833 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 101799 101833 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 101834 101835 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 101834 160824 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 160823 160824 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 160825 160862 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 160825 160862 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 160863 160864 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 160863 215485 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 215484 215485 . + . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 215486 215524 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 215486 215524 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 215525 215526 . + . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 215525 258440 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 258439 258440 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 258441 258473 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 258441 258473 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 258474 258475 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 258474 268681 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 268680 268681 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 268682 268693 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 268682 268693 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 268694 268695 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 268694 318956 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 318955 318956 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 318957 318977 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 318957 318977 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 318978 318979 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 318978 346203 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 346202 346203 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 346204 346223 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 346204 346223 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 346224 346225 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 346224 410996 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 410995 410996 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 410997 411008 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 410997 411008 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 411009 411010 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 411009 499767 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 499766 499767 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 499768 499785 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 499768 499785 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 499786 499787 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 499786 601459 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 610196 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 610195 610196 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 610197 610259 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 610197 610259 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 610260 610261 . + . intron_id 12 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 610260 654150 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 654149 654150 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 654151 654184 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 654151 654184 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 654185 654186 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 654185 666657 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 845360 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 845359 845360 . + . intron_id 13 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 845361 845392 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 845361 845392 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 845393 845394 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 845393 906925 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 906924 906925 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 906926 906946 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 906926 906946 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 906947 906948 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 906947 914155 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 914154 914155 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 914156 914170 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 914156 914170 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 914171 914172 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 914171 982306 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 982305 982306 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 982307 982343 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 982307 982343 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 982344 982345 . + . intron_id 18 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 982344 1011376 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1102420 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1102419 1102420 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1102421 1102457 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1102421 1102457 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1102458 1102459 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1102458 1112131 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112130 1112131 . + . intron_id 19 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1112132 1112147 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1112132 1112147 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1168480 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1168479 1168480 . + . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1168481 1168493 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1168481 1168493 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 62075 1168493 107 + . alignment_id 28 ; Query SPP00000007_1.0 ; Align 62075 13 30 ; Align 101800 24 33 ; Align 160827 36 36 ; Align 215486 48 39 ; Align 258441 61 33 ; Align 268682 72 12 ; Align 318957 76 21 ; Align 346204 83 18 ; Align 410998 90 9 ; Align 499769 94 15 ; Align 601461 100 15 ; Align 610198 106 60 ; Align 654152 127 33 ; Align 845361 138 30 ; Align 906927 149 18 ; Align 914157 156 12 ; Align 982308 161 36 ; Align 1102421 173 36 ; Align 1112134 186 12 ; Align 1168482 191 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 106 Query range: 3 -> 160 Target range: 54895 -> 1174803 4 : GlyArgLeuCysArg{Le} >>>> Target Intron 1 >>>> {u}LeuLy : 11 |||!:!:!!||||||{ } 193079 bp {!}:!!|| GlyGlnValCysArg{Gl}++ ++{y}IleLy 54896 : GGCCAGGTCTGCAGA{GG}gt.........................ag{G}ATTAA : 247996 12 : s >>>> Target Intron 2 >>>> ProAlaLeuLeuCysGlyAlaLeuAl : 20 | 39670 bp ||||||! !:!!!! !||||||.! s++ ++ProAlaGlnValTrpGlnAlaLeuTh 247997 : Ggt.........................agCCAGCCCAAGTGTGGCAGGCACTGAC : 287693 21 : a{A} >>>> Target Intron 3 >>>> {la}ProGlyLeuAlaGlyThrM : 28 !{!} 41367 bp {.!}|||||| !||||||||| r{G}++ +-{ly}ProGlyGluAlaGlyThrS 287694 : T{G}gt.........................aa{GT}CCTGGGGAGGCTGGGACAT : 329084 29 : etCysAla >>>> Target Intron 4 >>>> SerArgAspAspTrpArgC : 37 ||| ! 14115 bp :!!! ! :!!|||||| erCysLys++ ++AlaThrArgAsnTrpArgG 329085 : CATGCAAGgt.........................agGCGACCCGAAATTGGAGAC : 343226 38 : ysAlaArgSerMetHisGluPheSerAlaLysAspIle{As} >>>> Target : 50 !! !.!!! |||..!.!. !::!||| !:!!{.!} 1193 lnProSerGlyThrHisArgMetLeuSerLysLeuLeu{Gl}++ 343227 : AGCCCTCAGGCACACACAGGATGCTGAGTAAGCTCTTA{CA}gt............ : 343269 51 : Intron 5 >>>> {p}GlyHisMetVal{As} >>>> Target Intron 6 : 55 63 bp {.}...|||::: {! } 11779 bp ++{n}SerHisValTrp{Me}++ 343270 : .............ag{g}agtcatgtatgg{at}gt.................... : 462647 56 : >>>> {n}LeuAspLysTyrArgGlyPheVal{Cy} >>>> Target Intr : 64 { }||| ||||||||| { !} 30116 b ++{t}LeuLeuCysProArgGlyPheSer{Se}++ 462648 : .....ag{g}ctcctctgtccacggggattctcc{ag}gt................ : 474453 65 : on 7 >>>> {s}IleValThrAsnValAlaSerGlnUnkGlyLysThrGluVal : 78 p {!} !:!!! !|||:!! !..!!:!...!.! ! !...||| ++{r}ProLeuMetAsnIleLeuGlnArgAlaAlaCysIleSerVal 474454 : .........ag{C}CCCCTAATGAACATCCTGCAGCGGGCAGCCTGTATATCTGTT : 504607 79 : AsnTyrThrGlnLeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArgIl : 97 !.! ! !|||:!!! !|||..! !!!:: !! !|||!.!:!!!.!|| ThrValAspThrLeuIleValArgHisCysCysTrpLeuGlyCysAlaValHisIl 504608 : ACCGTGGATACGCTGATAGTGAGACACTGCTGTTGGTTGGGATGTGCTGTACATAT : 504664 98 : eLeuAlaPhe >>>> Target Intron 8 >>>> ProCysAsnGlnPheGl : 106 |! !!.!.!. 43711 bp !|||!.!||| || eProGlyMet++ ++GlyCysThrGlnArgGl 504665 : TCCCGGGATGgt.........................agGGCTGTACTCAAAGGGG : 548402 107 : yLysGlnGluProGlySerAsnGluGluIleLysGlu{P} >>>> Target In : 119 | !... ||||||......:::...::: :::{ } 67482 yAlaSerValProGlyLysLysLysAsnVal***Lys{G}++ 548403 : AGCGTctgttcctggaaaaaaaaaaaatgtttaaaaa{g}gt.............. : 548444 120 : tron 9 >>>> {he}AlaAlaGlyTyrAsn >>>> Target Intron 10 : 125 bp { }|||...|||||| 50717 bp ++{ly}AlaGlyGlyTyrTrp++ 548445 : ...........ag{gg}gctgggggataCTGGgt...................... : 615943 126 : >>>> >>>> Target Intron 11 >>>> ValLysPheAspMet{Ph} : 130 197115 bp :!!||||||:!! { } ++++ ++IleLysPheAsnSer{Ar}+ 615944 : ....aggt..........................agATAAAATTCAATTCC{AG}g : 863790 131 : >>>> Target Intron 12 >>>> {e} >>>> Target Intron 13 : 131 118517 bp { } 29069 bp + ++{g}++ 863791 : t..........................ag{g}gt...................... : 982310 132 : >>>> >>>> Target Intron 14 >>>> >>>> Target Intron : 131 100771 bp 60435 bp ++++ ++++ 982311 : ....aggt..........................aggt.................. : 1112150 132 : 15 >>>> SerLysIleCysValAsnGlyAspAspAlaHisProLeuTrpLysT : 146 |||:!!:!! !! ! ||| !..! ! |||||||||:!!! ++SerGlnLeuHisGlyAlaGlyProSerHisValProLeuTrpGlnS 1112151 : ........agTCCCAGCTCCACGGTGCAGGTCCCTCCCATGTGCCCCTCTGGCAGT : 1172628 147 : rpMetLysIleGlnProLysGlyLys{Gl} >>>> Target Intron 16 >> : 155 !..!||| !|||:!!{||} 2131 bp erProProProSerProTrpGlyGlu{Gl}++ 1172629 : CCCCTCCTCCTTCACCGTGGGGCGAA{GG}gt........................ : 1172659 156 : >> {y}IleLeuGlyAsnAla : 160 {|}:!!||||||::!:!! ++{y}LeuLeuGlySerSer 1172660 : ..ag{A}CTCCTGGGTTCCTCT : 1174803 vulgar: SPP00000007_1.0 3 160 . NIVO01030317.1:subseq(555646,1182752) 54895 1174803 + 106 M 5 15 S 0 2 5 0 2 I 0 193075 3 0 2 S 1 1 M 2 6 5 0 2 I 0 39666 3 0 2 M 9 27 S 0 1 5 0 2 I 0 41363 3 0 2 S 1 2 M 9 27 5 0 2 I 0 14111 3 0 2 M 19 57 S 0 2 5 0 2 I 0 119359 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 11775 3 0 2 S 1 1 M 8 24 S 0 2 5 0 2 I 0 30112 3 0 2 S 1 1 M 36 108 5 0 2 I 0 43707 3 0 2 M 18 54 S 0 1 5 0 2 I 0 67478 3 0 2 S 1 2 M 5 15 5 0 2 I 0 50713 3 0 2 5 0 2 I 0 197111 3 0 2 M 5 15 S 0 2 5 0 2 I 0 118513 3 0 2 S 1 1 5 0 2 I 0 29065 3 0 2 5 0 2 I 0 100767 3 0 2 5 0 2 I 0 60431 3 0 2 M 24 72 S 0 2 5 0 2 I 0 2127 3 0 2 S 1 1 M 5 15 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 54896 1174803 106 + . gene_id 29 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 54896 54912 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 54896 54912 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 54913 54914 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 54913 247991 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247990 247991 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247992 247998 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247992 247998 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247999 248000 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247999 287668 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 287667 287668 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 287669 287696 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 287669 287696 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 287697 287698 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 287697 329063 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 329062 329063 . + . intron_id 2 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 329064 329092 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 329064 329092 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 329093 329094 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 329093 343207 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 343206 343207 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 343208 343266 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 343208 343266 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 343267 343268 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 343267 462629 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462644 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462644 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462645 462646 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462645 474423 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 474422 474423 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 474424 474450 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 474424 474450 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 474451 474452 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 474451 504566 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 504565 504566 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 504567 504675 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 504567 504675 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 504676 504677 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 504676 548386 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 548385 548386 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 548387 548441 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 548387 548441 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 548442 548443 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 548442 615923 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 615922 615923 . + . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 615924 615940 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 615924 615940 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 615941 615942 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 615941 666657 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 863772 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 982306 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 982305 982306 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 982307 982307 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 982307 982307 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 982308 982309 . + . intron_id 13 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 982308 1011376 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1112147 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1172582 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1172581 1172582 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1172583 1172656 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1172583 1172656 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1172657 1172658 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1172657 1174787 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1174786 1174787 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1174788 1174803 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1174788 1174803 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 54896 1174803 106 + . alignment_id 29 ; Query SPP00000007_1.0 ; Align 54896 4 15 ; Align 247993 10 6 ; Align 287669 12 27 ; Align 329066 22 27 ; Align 343208 31 57 ; Align 462631 51 12 ; Align 474425 56 24 ; Align 504568 65 108 ; Align 548387 101 54 ; Align 615926 120 15 ; Align 863773 125 15 ; Align 1172583 131 72 ; Align 1174789 156 15 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 105 Query range: 29 -> 195 Target range: 20891 -> 940791 30 : AlaSerArgAspAspTrpArgCysAlaArgSerMetHisGluPheSer >>>> Tar : 46 |||.!!|||...! ||| !!||| !|||:!! !...! ! !..! AlaGlyArgSerGlyTrpGlyCysArgArgAlaTrpArgGlyHisGln++ 20892 : GCGGGCAGATCAGGGTGGGGGTGTAGGAGGGCCTGGAGGGGACATCAGgt........ : 20942 47 : get Intron 1 >>>> AlaLysAspIleAspGlyHisMetVal{A} >>>> Ta : 55 14957 bp ||||||!!::!! ||||||:!!! !{:} ++AlaLysGluValThrGlyHisLeuGly{A}+- 20943 : .................agGCCAAAGAGGTCACAGGGCACCTGGGA{G}ga....... : 35927 56 : rget Intron 2 >>>> {sn}LeuAspLysTyrArgGlyPheValCysIle{Va} : 66 18954 bp {!!}||| !!!. !|||||| ||||||! !{! } ++{sp}LeuIleAsnAlaArgGlyGlnValCysArg{Gl} 35928 : ..................ag{AT}CTCATCAATGCCAGGGGCCAGGTCTGCAGA{GG} : 54911 67 : >>>> Target Intron 3 >>>> {l}ThrAsnValAlaSerGln >>>> T : 73 3130 bp {!} ! !|||..!|||||| ++ ++{y}LeuAlaValCysSerGln++ 54912 : gt.........................ag{G}CTGGCTGTGTGCTCTCAGgt...... : 58064 74 : arget Intron 4 >>>> UnkGlyLysThrGluVal{A} >>>> Target In : 79 3593 bp .!!||| !:!!..!{|} 50771 ++GlySerLysLeuGlnThr{A}++ 58065 : ...................agGGGAGCAAGTTACAGACA{A}gt.............. : 61676 80 : tron 5 >>>> {sn}TyrThrGlnLeuValAsp >>>> Target Intron 6 : 86 bp {||}! ! !...|||:!!||| 10883 bp ++{sn}LeuIleSerLeuIleAsp-+ 61677 : ...........ag{AT}TTAATTTCTCTAATAGATat..................... : 112467 87 : >>>> LeuHis{Al} >>>> Target Intron 7 >>>> {a}ArgTyrAlaG : 92 |||{!.} 2543 bp {!} !! !| ++AlaHis{Gl}++ ++{y}ValLeuLeuG 112468 : ....aggcccaC{GG}gt.........................ag{G}GTCTTATTGG : 125909 93 : luCysGly{Le} >>>> Target Intron 8 >>>> {u}ArgIleLeuAlaPh : 100 ||||||||{ } 3195 bp {!}!.!:!: !||| luCysGly{Se}++ +-{r}HisLeuSerAlaGl 125910 : AATGTGGG{AG}gt.........................ac{T}CACCTGAGTGCAGA : 129128 101 : eProCysAsnGlnPheGlyLysGln >>>> Target Intron 9 >>>> GluP : 110 ||||||:::|||.!...! !||| 1199 bp |||| uProCysSerGlnLeuAsnGlyGln++ ++GluP 129129 : GCCCTGCTCACAGCTGAACGGGCAGgt.........................agGAGC : 130357 111 : roGlySer >>>> Target Intron 10 >>>> AsnGluGluIleLysGlu : 119 |||||..! 16215 bp |||||| !!..:!! roGlyGln++ ++AlaGluGluProSerGln++ 130358 : CCGGCCAGgt..........................agGCAGAGGAACCCAGCCAGgt : 146599 120 : >>>> Target Intron 11 >>>> PheAlaAlaGlyTyrAsnValLysPheAsp : 128 81240 bp ||| |||||| ::: ||| ++PheIleLysGlyTyr***Ile***PhePro 146600 : ..........................agtttatcaaaggatattaaatatagttccct : 227866 129 : MetPheSer{L} >>>> Target Intron 12 >>>> {ys}{Il} >>>> T : 133 :::... {|} 19354 bp {||}{! } ValLeuTyr{L}++ ++{ys}{Th}++ 227867 : gtgctatac{a}gt..........................ag{AA}{AC}gt...... : 247239 134 : arget Intron 13 >>>> {e}CysValAsnGly{A} >>>> Target Intr : 138 23484 bp {!}|||..!||||||{|} 16066 b ++{r}CysAlaAsnGly{A}++ 247240 : ....................ag{c}tgcgCCAACGGA{G}gt................ : 270737 139 : on 14 >>>> {sp}AspAlaHisProLeuTrpLys >>>> Target Intron : 146 p {||}.!. !||||||||| ::: 9359 bp ++{sp}GlnPheHisProLeuGlnArg++ 270738 : ..........ag{AC}CAGTTCCACCCTCTGcagcgggt................... : 286826 147 : 15 >>>> TrpMetLysIleGlnProLys{G} >>>> Target Intron 16 > : 153 |||.!. ! !! ||||||{|} 64320 bp ++TrpPheLeuAlaProProLys{G}+- 286827 : .......agTGGTTCCTGGCACCTCCAAAA{G}gg....................... : 296207 154 : >>> {ly}LysGlyIleLeuGlyAsnAlaIle{L} >>>> Target Intron 1 : 162 {||}|||!.!:!:|||..!.!.|||! !{ } 117592 bp ++{ly}LysAlaValLeuSerGlnAlaThr{A}++ 296208 : ...ag{GG}AAAGCCGTGCTTTCACAGGCAACT{G}gt.................... : 360554 163 : 7 >>>> {ys}TrpAsnPheThrLysPheLeu >>>> Target Intron 18 > : 170 { !}|||!.!!:: !::!.!!:!! 188491 bp ++{la}TrpThrTrpAspArgLeuMet++ 360555 : ......ag{CG}TGGACTTGGGACCGGCTCATGgt....................... : 478169 171 : >>> >>>> Target Intron 19 >>>> IleAspLysAsnGly{Cy} >> : 175 44582 bp !|||!:!.!.|||{ !} ++++ ++AspAspArgGluGly{Se}++ 478170 : ...aggt..........................agGATGACAGGGAGGGA{AG}gt.. : 711259 176 : >> Target Intron 20 >>>> {s}Val{V} >>>> Target Intron 21 : 177 103919 bp {!}|||{|} 10393 bp ++{r}Val{V}++ 711260 : ........................ag{C}GTT{G}gt..................... : 815183 178 : >>>> {al}LysArgTyrGly{P} >>>> Target Intron 22 >>>> {r : 182 {||} ||||||{ } 115162 bp { ++{al}ValMetTyrGly{T}++ ++{y 815184 : .....ag{ta}gtcatgtatgga{t}gt..........................ag{a : 940751 183 : o}MetGluGluProLeuValIleGluLysAspLeuProHis : 195 !} :::|||||| ::::::||| ::: ||| r}TrpGlyLysProLeuLysValLysLysLysIleSerHis 940752 : t}tggggaaaaccactaaaggtaaaaaaaaaaatttcacac : 940791 vulgar: SPP00000007_1.0 29 195 . NIVO01030317.1:subseq(555646,1182752) 20891 940791 + 105 M 16 48 5 0 2 I 0 14953 3 0 2 M 9 27 S 0 1 5 0 2 I 0 18950 3 0 2 S 1 2 M 10 30 S 0 2 5 0 2 I 0 3126 3 0 2 S 1 1 M 6 18 5 0 2 I 0 3589 3 0 2 M 6 18 S 0 1 5 0 2 I 0 50767 3 0 2 S 1 2 M 6 18 5 0 2 I 0 10879 3 0 2 M 2 6 S 0 2 5 0 2 I 0 2539 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 3191 3 0 2 S 1 1 M 13 39 5 0 2 I 0 1195 3 0 2 M 4 12 5 0 2 I 0 16211 3 0 2 M 6 18 5 0 2 I 0 81236 3 0 2 M 13 39 S 0 1 5 0 2 I 0 19350 3 0 2 S 1 4 5 0 2 I 0 23480 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 16062 3 0 2 S 1 2 M 7 21 5 0 2 I 0 9355 3 0 2 M 7 21 S 0 1 5 0 2 I 0 64316 3 0 2 S 1 2 M 8 24 S 0 1 5 0 2 I 0 117588 3 0 2 S 1 2 M 7 21 5 0 2 I 0 188487 3 0 2 5 0 2 I 0 44578 3 0 2 M 5 15 S 0 2 5 0 2 I 0 103915 3 0 2 S 1 1 M 1 3 S 0 1 5 0 2 I 0 10389 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 115158 3 0 2 S 1 2 M 13 39 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 20892 940791 105 + . gene_id 30 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 20892 20939 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 20892 20939 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 20940 20941 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 20940 35896 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 35895 35896 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 35897 35924 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 35897 35924 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 35925 35926 . + . intron_id 2 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 35925 54878 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 54877 54878 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 54879 54912 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 54879 54912 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 54913 54914 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 54913 58042 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 58041 58042 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 58043 58061 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 58043 58061 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 58062 58063 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 58062 61654 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 61653 61654 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 61655 61673 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 61655 61673 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 61674 61675 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 61674 112444 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 112443 112444 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 112445 112464 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 112445 112464 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 112465 112466 . + . intron_id 6 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 112465 123347 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 123346 123347 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 123348 123355 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 123348 123355 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 123356 123357 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 123356 125898 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 125897 125898 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 125899 125919 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 125899 125919 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 125920 125921 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 125920 129114 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 129113 129114 . + . intron_id 7 ; splice_site "AC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 129115 129154 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 129115 129154 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 129155 129156 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 129155 130353 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 130352 130353 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 130354 130365 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 130354 130365 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 130366 130367 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 130366 146580 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146579 146580 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146581 146598 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146581 146598 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146599 146600 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146599 227838 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 227837 227838 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 227839 227878 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 227839 227878 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 227879 227880 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 227879 247232 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247231 247232 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247233 247236 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247233 247236 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247237 247238 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247237 270720 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 270719 270720 . + . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 270721 270734 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 270721 270734 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 270735 270736 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 270735 286800 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 286799 286800 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 286801 286823 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 286801 286823 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 286824 286825 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 286824 296182 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 296181 296182 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 296183 296204 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 296183 296204 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 296205 296206 . + . intron_id 16 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 296205 360524 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 360523 360524 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 360525 360551 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 360525 360551 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 360552 360553 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 360552 478143 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 478142 478143 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 478144 478166 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 478144 478166 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 478167 478168 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 478167 666657 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 711239 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 711238 711239 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 711240 711256 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 711240 711256 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 711257 711258 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 711257 815175 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 815174 815175 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 815176 815180 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 815176 815180 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 815181 815182 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 815181 825573 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 825572 825573 . + . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 825574 825588 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 825574 825588 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 825589 825590 . + . intron_id 22 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 825589 940750 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 940749 940750 . + . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 940751 940791 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 940751 940791 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 20892 940791 105 + . alignment_id 30 ; Query SPP00000007_1.0 ; Align 20892 30 48 ; Align 35897 46 27 ; Align 54881 56 30 ; Align 58044 67 18 ; Align 61655 73 18 ; Align 112447 80 18 ; Align 123348 86 6 ; Align 125900 89 18 ; Align 129116 96 39 ; Align 130354 109 12 ; Align 146581 113 18 ; Align 227839 119 39 ; Align 270722 134 12 ; Align 286803 139 21 ; Align 296183 146 21 ; Align 360527 154 24 ; Align 478146 163 21 ; Align 711240 170 15 ; Align 815177 176 3 ; Align 825576 178 12 ; Align 940753 183 39 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 107 Query range: 6 -> 195 Target range: 13652 -> 891328 7 : CysArgLeuLeuLysProAlaLeuLeuCysGly{A} >>>> Target Intron 1 : 18 |||! !! !! ! !||| !! !:!!|||..!{|} 2093 bp CysProSerGlnProProTrpProValCysSer{A}++ 13653 : TGCCCGTCGCAGCCGCCGTGGCCTGTTTGCTCA{G}gt.................... : 13689 19 : >>>> {la}LeuAlaAlaProGlyLeuAlaGlyThrMetCys{A} >>>> Targ : 30 {||}:!!:!!::!|||||| ! !.!! !!!!: !!{|} ++{la}ValSerSerProGlyAspLeuSerProIleSer{A}++ 13690 : .....ag{CT}GTGTCTAGCCCTGGGGACCTCAGTCCCATCAGT{G}gt......... : 15818 31 : et Intron 2 >>>> {la}SerArgAspAspTrpArgCysAla{Ar} >>>> T : 39 47900 bp {||}! ! ! !! !||| !||||||{||} +-{la}LeuAlaCysGlyTrpPheCysAla{Ar}++ 15819 : ................aa{CA}TTGGCCTGTGGCTGGTTTTGTGCC{AG}gt...... : 63746 40 : arget Intron 3 >>>> {g}SerMetHis{G} >>>> Target Intron 4 : 43 10360 bp {|}|||:!:! {!} 3292 bp ++{g}SerLeuPro{G}++ 63747 : ...................ag{A}TCCCTCCCA{G}gt.................... : 74117 44 : >>>> {lu}PheSerAlaLysAspIleAspGly{H} >>>> Target Intron : 52 { !}|||||| !!:!!!:||| !.!{ } 50327 bp ++{ly}PheSerLeuArgGluIleThrAla{G}++ 74118 : .....ag{GG}TTCTCATTAAGGGAGATCACAGCA{G}gt.................. : 77436 53 : 5 >>>> {is}MetValAsnLeuAspLysTyrArgGlyPheValCysIleVal{T} : 67 { }:!!||| |||! ! !:!!!:!||| !!.!|||||||||{:} -+{ly}LeuValProLeuAlaLeuHisLysGlyProAlaCysIleVal{S} 77437 : .......gg{GG}CTGGTTCCACTAGCTCTGCATAAAGGTCCTGCCTGCATCGTG{T} : 127805 68 : >>>> Target Intron 6 >>>> {hr}AsnValAlaSerGlnUnkGlyLysT : 76 49834 bp {!!} !! !! !...... !|||! ++ ++{er}LeuSerAspPheSerSerPheLysA 127806 : gt.........................ag{CT}CTGAGCGATTTCTCCAGCTTCAAAA : 177666 77 : hrGluValAsnTyrThrGlnLeuVal{As} >>>> Target Intron 7 >>>> : 85 !.!.:!!||||||:!!.!. !|||{..} 20058 bp rgHisIleAsnTyrSerAsnGluVal{Se}+- + 177667 : GGCACATTAATTACTCTAATGAGGTC{AG}ga.........................a : 197751 86 : {p}LeuHisAlaArgTyrAlaGluCys{Gl} >>>> Target Intron 8 >>> : 94 {!}||| ! !||| !.!!||||||{||} 10176 bp +{r}LeuAlaGlnArgGlyThrGluCys{Gl}-+ 197752 : g{C}CTGGCCCAGAGAGGCACAGAGTGT{GG}ct........................ : 197782 95 : > {y}LeuArgIleLeuAlaPheProCysAsnGlnPheGlyLys >>>> Target : 108 {|}:!!||| ||| ! !|||! !||| !::|||||| 10 +-{y}IleArgGlyLeuGlnAlaProPheAsnPheTrpGlyLys+- 197783 : .ac{T}ATTCGGGGGCTCCAGGCCCCCTTCAATTTCTGGGGTAAGga........... : 207998 109 : Intron 9 >>>> GlnGluPro >>>> Target Intron 10 >>>> Gly : 111 322 bp ::! ||| 13189 bp ||| ++ArgProPro++ ++Gly 207999 : ..............agAGACCTCCTgt..........................agGGG : 231516 112 : SerAsnGlu{Gl} >>>> Target Intron 11 >>>> {u}IleLys >>>> : 118 |||...|||{:!} 16462 bp {!}|||||| SerGlyGlu{Ly}-+ ++{s}IleLys++ 231517 : TCAGGGGAG{AA}ct..........................ag{G}ATTAAGgt.... : 248001 119 : Target Intron 12 >>>> GluPheAlaAla{G} >>>> Target Intro : 122 20549 bp ! ! ||||||{!} 1384 bp ++Ala***AlaAla{G}++ 248002 : ......................agGCCTGAGCAGca{g}gt................. : 268563 123 : n 13 >>>> {ly}TyrAsnValLysPheAspMetPheSerLysIleCysValAsnG : 137 { !} ! !||| !|||:!:!:: !! :!: !..!| ++{lu}LeuAlaValAlaHisAspLeuTrpProGlyLeuLeuCysArgG 268564 : .........ag{AG}CTCGCTGTGGCCCATGACCTTTGGCCTGGCCTGCTGTGCCGTG : 269989 138 : lyAspAspAlaHis >>>> Target Intron 14 >>>> ProLeu{Tr} >> : 144 |||||||||||||| 28836 bp |||:!!{||} lyAspAspAlaHis+- ++ProIle{Tr}++ 269990 : GAGATGATGCACATgg..........................agCCAATA{TG}gt.. : 298850 145 : >> Target Intron 15 >>>> {p}LysTrp{Me} >>>> Target Intro : 147 2458 bp {|} |||{:!} 2374 bp ++{p}CysTrp{Le}++ 298851 : ........................ag{G}TGTTGG{CT}gt................. : 301317 148 : n 16 >>>> {t}LysIleGlnProLysGlyLysGlyIleLeuGlyAsnAlaIleLy : 162 {!} :!::!!||| !! !! !|||:!:! !|||... ! ! ++{u}ProValGluProProAspMetGlyValTrpGlyGlyLeuAlaGl 301318 : .........ag{G}CCTGTGGAACCGCCGGATATGGGGGTGTGGGGCGGGCTTGCTGG : 303732 163 : s{T} >>>> Target Intron 17 >>>> {rp}AsnPheThr{L} >>>> T : 167 {|} 197929 bp {||}|||||| {:} y{T}++ ++{rp}AsnPhePro{G}++ 303733 : T{T}gt..........................ag{gg}aattttcca{g}gt...... : 501679 168 : arget Intron 18 >>>> {ys}PheLeuIleAspLysAsnGlyCysValVal{L : 178 152474 bp {!!}... ! !... ! |||||| ! !{ ++{lu}LeuSerTyrSerPro***GlyCysTyrPro{G 501680 : ....................ag{aa}ttgAGTTATTCGCCTTAGGGTTGTTATCCA{G : 654183 179 : y} >>>> Target Intron 19 >>>> {s}ArgTyr{G} >>>> Target : 181 } 28375 bp { } |||{|} 305 l}++ ++{y}SerTyr{G}++ 654184 : G}gt..........................ag{t}tccTAT{G}gt............ : 682570 182 : Intron 20 >>>> {ly}ProMetGluGluProLeuValIleGlu{L} >>>> T : 191 47 bp {||}|||:!!..!:!!||| !:!!:!! !{!} ++{ly}ProValArgLysProGlyIleValPro{A}++ 682571 : ..............ag{GC}CCCGTGCGGAAGCCGGGAATCGTACCA{A}gt...... : 713147 192 : arget Intron 21 >>>> {ys}AspLeuProHis : 195 178170 bp {:!}|||||||||||| ++{rg}AspLeuProHis 713148 : ....................ag{gg}gatcttccccac : 891328 vulgar: SPP00000007_1.0 6 195 . NIVO01030317.1:subseq(555646,1182752) 13652 891328 + 107 M 11 33 S 0 1 5 0 2 I 0 2089 3 0 2 S 1 2 M 11 33 S 0 1 5 0 2 I 0 47896 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 10356 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 3288 3 0 2 S 1 2 M 8 24 S 0 1 5 0 2 I 0 50323 3 0 2 S 1 2 M 14 42 S 0 1 5 0 2 I 0 49830 3 0 2 S 1 2 M 17 51 S 0 2 5 0 2 I 0 20054 3 0 2 S 1 1 M 8 24 S 0 2 5 0 2 I 0 10172 3 0 2 S 1 1 M 13 39 5 0 2 I 0 10318 3 0 2 M 3 9 5 0 2 I 0 13185 3 0 2 M 4 12 S 0 2 5 0 2 I 0 16458 3 0 2 S 1 1 M 2 6 5 0 2 I 0 20545 3 0 2 M 4 12 S 0 1 5 0 2 I 0 1380 3 0 2 S 1 2 M 19 57 5 0 2 I 0 28832 3 0 2 M 2 6 S 0 2 5 0 2 I 0 2454 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 2370 3 0 2 S 1 1 M 15 45 S 0 1 5 0 2 I 0 197925 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 152470 3 0 2 S 1 2 M 10 30 S 0 2 5 0 2 I 0 28371 3 0 2 S 1 1 M 2 6 S 0 1 5 0 2 I 0 30543 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 178166 3 0 2 S 1 2 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 13653 891328 107 + . gene_id 31 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 13653 13686 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 13653 13686 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 13687 13688 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 13687 15779 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 15778 15779 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 15780 15815 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 15780 15815 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 15816 15817 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 15816 63715 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 63714 63715 . + . intron_id 1 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 63716 63743 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 63716 63743 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 63744 63745 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 63744 74103 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 74102 74103 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 74104 74114 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 74104 74114 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 74115 74116 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 74115 77406 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 77405 77406 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 77407 77433 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 77407 77433 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 77434 77435 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 77434 127760 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 127759 127760 . + . intron_id 4 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 127761 127805 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 127761 127805 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 127806 127807 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 127806 177639 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 177638 177639 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 177640 177694 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 177640 177694 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 177695 177696 . + . intron_id 7 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 177695 197752 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 197751 197752 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 197753 197779 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 197753 197779 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 197780 197781 . + . intron_id 8 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 197780 207955 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 207954 207955 . + . intron_id 7 ; splice_site "aC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 207956 207995 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 207956 207995 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 207996 207997 . + . intron_id 9 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 207996 218317 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 218316 218317 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 218318 218326 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 218318 218326 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 218327 218328 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 218327 231515 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 231514 231515 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 231516 231529 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 231516 231529 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 231530 231531 . + . intron_id 11 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 231530 247991 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247990 247991 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247992 247998 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247992 247998 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247999 248000 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247999 268547 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 268546 268547 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 268548 268560 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 268548 268560 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 268561 268562 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 268561 269944 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 269943 269944 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 269945 270003 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 269945 270003 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 270004 270005 . + . intron_id 14 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 270004 298839 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 298838 298839 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 298840 298847 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 298840 298847 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 298848 298849 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 298848 301305 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 301304 301305 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 301306 301314 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 301306 301314 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 301315 301316 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 301315 303688 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 303687 303688 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 303689 303735 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 303689 303735 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 303736 303737 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 303736 501664 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 501663 501664 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 501665 501676 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 501665 501676 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 501677 501678 . + . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 501677 654150 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 654149 654150 . + . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 654151 654184 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 654151 654184 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 654185 654186 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 654185 682559 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 682558 682559 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 682560 682567 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 682560 682567 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 682568 682569 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 682568 713114 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 713113 713114 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 713115 713144 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 713115 713144 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 713145 713146 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 713145 891314 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 891313 891314 . + . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 891315 891328 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 891315 891328 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 13653 891328 107 + . alignment_id 31 ; Query SPP00000007_1.0 ; Align 13653 7 33 ; Align 15782 19 33 ; Align 63718 31 24 ; Align 74105 40 9 ; Align 77409 44 24 ; Align 127763 53 42 ; Align 177642 68 51 ; Align 197754 86 24 ; Align 207957 95 39 ; Align 218318 108 9 ; Align 231516 111 12 ; Align 247993 116 6 ; Align 268548 118 12 ; Align 269947 123 57 ; Align 298840 142 6 ; Align 301307 145 6 ; Align 303690 148 45 ; Align 501667 164 9 ; Align 654153 168 30 ; Align 682561 179 6 ; Align 713117 182 27 ; Align 891317 192 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 104 Query range: 1 -> 186 Target range: 11894 -> 1162453 2 : SerLeuGlyArgLeu >>>> Target Intron 1 >>>> CysArgLeuLeu : 10 |||||||||!!!||| 11900 bp |||||||||! ! SerLeuGlySerLeu++ ++CysArgLeuTrp 11895 : AGCTTGGGCAGCCTGgt.........................agTGCCGGCTGTGG : 23819 11 : LysProAlaLeu{L} >>>> Target Intron 2 >>>> {eu}CysGlyAl : 18 |||!.! !{ } 90319 bp { !} !!||| ! GlyProGlySer{A}++ ++{la}GlyGlyPr 23820 : GGTCCAGGGAGT{G}gt.........................ag{CC}GGCGGGCC : 114162 19 : aLeuAlaAlaProGlyLeuAlaGlyThrMetCysAlaSerArg{As} >>>> Ta : 33 !! !|||||||||!.!|||||||||.!! ! |||.!!|||{..} oArgAlaAlaProAlaLeuAlaGlyAlaGlyArgAlaGlyArg{Se}++ 114163 : CCGGGCAGCTCCGGCGCTGGCGGGAGCCGGCCGAGCTGGACGA{AG}gt....... : 114211 34 : rget Intron 3 >>>> {p}AspTrpArgCysAla >>>> Target Intr : 39 69070 bp {!}||||||..! !! ! 55746 b +-{r}AspTrpGluThrGlu++ 114212 : ..................ac{T}GACTGGGAAACTGAGgt................ : 183297 40 : on 4 >>>> ArgSerMetHisGluPheSerAlaLysAspIle{As} >>>> T : 50 p ||||||:!!.!. ! ! !.!! !!::!!{:!} ++ArgSerValGluProArgMetThrProGluVal{As}++ 183298 : .........agAGGTCAGTGGAGCCACGGATGACACCCGAGGTC{AA}gt...... : 239078 51 : arget Intron 5 >>>> {p}GlyHisMetValAsn{Le} >>>> Target : 56 31716 bp {!}||||||:!: ! !{ } 120 ++{n}GlyHisLeuArgPro{Ar}++ 239079 : ...................ag{T}GGACATCTACGTCCC{AG}gt........... : 270812 57 : Intron 6 >>>> {u}AspLysTyrArgGlyPheValCysIleValThr{A} : 68 259 bp {!} ||| |||||| ! !:!!||||||{.} +-{g}LysLysThrArgGlyGlyLysThrLeuValThr{G}+ 270813 : ..............aa{a}aaaaaaacaagaggagGAAAAACTTTAGTTACT{G}g : 391104 69 : >>>> Target Intron 7 >>>> {sn}ValAlaSerGlnUnkGlyLys{Th : 76 39227 bp {..}||||||:!!||| |||:!!{! + -+{ly}ValAlaAlaGlnValGlyGln{Ar 391105 : t.........................tg{GG}GTTGCTGCACAAGTCGGACAG{AG : 430354 77 : } >>>> Target Intron 8 >>>> {r}GluValAsnTyr{T} >>>> T : 81 } 113612 bp {!}:::... |||{.} }++ ++{g}GlnThrLeuTyr{V}++ 430355 : }gt.........................ag{g}cagactctttac{g}gt...... : 543984 82 : arget Intron 9 >>>> {hr}GlnLeuValAspLeuHisAlaArgTyrAlaG : 92 23365 bp {.!}...||||||...||| !||| !.!! +-{al}AsnLeuValSerLeuLeu***ArgValGlyG 543985 : ...................aa{tg}aatttgGTCTCACTTTTGTAAAGAGTGGGGG : 567379 93 : luCysGly >>>> Target Intron 10 >>>> LeuArgIleLeuAlaPhe : 100 !|||..! 2676 bp !!!!:! !|||||| lyCysSer++ ++PheGlnGlySerAlaPhe 567380 : GGTGTTCGgt..........................agTTTCAGGGGAGCGCCTTT : 570079 101 : ProCysAsnGlnPhe{Gl} >>>> Target Intron 11 >>>> {y}LysG : 108 ||| ! :!!! !{||} 2869 bp {|}|||! ProArgAspProHis{Gl}++ -+{y}LysL 570080 : CCACGAGACCCTCAC{GG}gt..........................tg{G}AAAC : 572972 109 : lnGlu{Pr} >>>> Target Intron 12 >>>> {o}GlySerAsnGluGl : 115 !.!.{ } 28480 bp {!}||||||::::::.. euHis{Ar}++ ++{g}GlySerSerGlnSe 572973 : TACAC{AG}gt..........................ag{g}ggatcttcccaatc : 601473 116 : u{Il} >>>> Target Intron 13 >>>> {e}LysGluPheAlaAlaGly : 122 .{! } 6380 bp {!}!.. ||||||||| r{Ar}++ ++{g}SerIlePheAlaAlaPro 601474 : c{ag}gt..........................ag{A}AGCATTtttgcagcccca : 607874 123 : TyrAsnValLys{Ph} >>>> Target Intron 14 >>>> {e}AspMetP : 130 ::: ! !{ } 18441 bp { }|||... GluSerHisGly{Gl}++ ++{y}AspGlnT 607875 : gagagccACGGA{GG}gt..........................ag{g}gatcaaa : 626339 131 : heSerLysIleCysValAsn{Gl} >>>> Target Intron 15 >>>> {y : 137 ... |||:::...{ !} 161824 bp {! hrGlnLeuSerCysIleGly{Ar}++ ++{g 626340 : cccagctctcctgcattggc{ag}gt..........................ag{G : 788186 138 : }AspAspAlaHisProLeuTrpLysTrpMet >>>> Target Intron 16 > : 148 }...!!: !!||| |||::: ::: 171087 bp }SerGluProHisSerArgTrpArgSerLeu++ 788187 : }TCAGAACCCCACtccagatggaggagcctggt....................... : 788219 149 : >>> LysIleGlnProLysGlyLys >>>> Target Intron 17 >>>> : 155 :!!:!!!!.||| |||::! 22982 bp ++GlnValHisProAlaGlyArg+- ++ 788220 : ...agCAAGTCCACCCAGCTGGCCGGga..........................ag : 982305 156 : GlyIleLeu >>>> Target Intron 18 >>>> >>>> Target Int : 158 ||| ||| 29061 bp 86371 GlyGluLeu++ ++++ 982306 : ggTGAGCTGgt..........................aggt............... : 1011379 159 : ron 19 >>>> GlyAsnAlaIleLysTrpAsnPheThrLys >>>> Target : 168 bp |||::: :::...::: ::: 14 ++GlySerAsnLeuSerPheLeuHisTrpGln++ 1011380 : ...........agggatcgaacctgagtttcctacattggcaggt........... : 1097780 169 : Intron 20 >>>> >>>> Target Intron 21 >>>> PheLeuIle : 170 370 bp 46691 bp ! |||:!! ++++ ++***LeuVal 1097781 : ...............aggt..........................agTGACTTGTA : 1158845 171 : AspLysAsnGlyCysValValLysArgTyr{G} >>>> Target Intron 22 : 181 ||| ...||||||:!! ! ! !! !{|} 3558 bp AspPheGlyGlyCysLeuSerGlyAspSer{G}++ 1158846 : GATTTTGGGGGGTGTTTGAGTGGAGACTCT{G}gt..................... : 1158881 182 : >>>> {ly}ProMetGluGluPro : 186 {||}|||! !!:||| ++{ly}ProThrIleAspPro 1158882 : .....ag{GC}CCCACCATCGACCCC : 1162453 vulgar: SPP00000007_1.0 1 186 . NIVO01030317.1:subseq(555646,1182752) 11894 1162453 + 104 M 5 15 5 0 2 I 0 11896 3 0 2 M 8 24 S 0 1 5 0 2 I 0 90315 3 0 2 S 1 2 M 17 51 S 0 2 5 0 2 I 0 69066 3 0 2 S 1 1 M 5 15 5 0 2 I 0 55742 3 0 2 M 11 33 S 0 2 5 0 2 I 0 31712 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 120255 3 0 2 S 1 1 M 11 33 S 0 1 5 0 2 I 0 39223 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 113608 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 23361 3 0 2 S 1 2 M 13 39 5 0 2 I 0 2672 3 0 2 M 11 33 S 0 2 5 0 2 I 0 2865 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 28476 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 6376 3 0 2 S 1 1 M 10 30 S 0 2 5 0 2 I 0 18437 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 161820 3 0 2 S 1 1 M 10 30 5 0 2 I 0 171083 3 0 2 M 7 21 5 0 2 I 0 22978 3 0 2 M 3 9 5 0 2 I 0 29057 3 0 2 5 0 2 I 0 86367 3 0 2 M 10 30 5 0 2 I 0 14366 3 0 2 5 0 2 I 0 46687 3 0 2 M 13 39 S 0 1 5 0 2 I 0 3554 3 0 2 S 1 2 M 5 15 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 11895 1162453 104 + . gene_id 32 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 11895 11909 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 11895 11909 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 11910 11911 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 11910 23809 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 23808 23809 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 23810 23834 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 23810 23834 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 23835 23836 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 23835 114153 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 114152 114153 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 114154 114208 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 114154 114208 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 114209 114210 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 114209 183278 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 183277 183278 . + . intron_id 2 ; splice_site "AC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 183279 183294 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 183279 183294 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 183295 183296 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 183295 239040 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 239039 239040 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 239041 239075 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 239041 239075 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 239076 239077 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 239076 270791 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 270790 270791 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 270792 270809 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 270792 270809 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 270810 270811 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 270810 391068 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 391067 391068 . + . intron_id 5 ; splice_site "aa" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 391069 391103 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 391069 391103 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 391104 391105 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 391104 430330 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 430329 430330 . + . intron_id 6 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 430331 430355 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 430331 430355 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 430356 430357 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 430356 543967 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 543966 543967 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 543968 543981 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 543968 543981 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 543982 543983 . + . intron_id 9 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 543982 567346 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 567345 567346 . + . intron_id 8 ; splice_site "aa" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 567347 567387 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 567347 567387 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 567388 567389 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 567388 570063 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 570062 570063 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 570064 570098 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 570064 570098 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 570099 570100 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 570099 572967 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 572966 572967 . + . intron_id 10 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 572968 572979 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 572968 572979 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 572980 572981 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 572980 601459 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 607857 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 607856 607857 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 607858 607890 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 607858 607890 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 607891 607892 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 607891 626331 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 626330 626331 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 626332 626361 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 626332 626361 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 626362 626363 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 626362 788185 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 788184 788185 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 788186 788216 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 788186 788216 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 788217 788218 . + . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 788217 959303 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 959302 959303 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 959304 959324 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 959304 959324 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 959325 959326 . + . intron_id 17 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 959325 982306 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 982305 982306 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 982307 982315 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 982307 982315 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 982316 982317 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 982316 1011376 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1097747 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1097746 1097747 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1097748 1097777 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1097748 1097777 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1097778 1097779 . + . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1097778 1112147 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1158838 . + . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1158837 1158838 . + . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1158839 1158878 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1158839 1158878 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1158879 1158880 . + . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1158879 1162436 . + . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1162435 1162436 . + . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1162437 1162453 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1162437 1162453 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 11895 1162453 104 + . alignment_id 32 ; Query SPP00000007_1.0 ; Align 11895 2 15 ; Align 23810 7 24 ; Align 114156 16 51 ; Align 183280 34 15 ; Align 239041 39 33 ; Align 270793 51 15 ; Align 391070 57 33 ; Align 430333 69 21 ; Align 543969 77 12 ; Align 567349 82 39 ; Align 570064 95 33 ; Align 572969 107 9 ; Align 601461 111 15 ; Align 607859 117 30 ; Align 626333 128 27 ; Align 788187 138 30 ; Align 959304 148 21 ; Align 982307 155 9 ; Align 1097748 158 30 ; Align 1158839 168 39 ; Align 1162439 182 15 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 106 Query range: 3 -> 183 Target range: 1882 -> 1125426 4 : GlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuAlaAlaPr : 22 |||!:! !||| !! !! !:!! !!.!||||||!! ||| ::: GlyLysArgCysGlyAsnProGlnGluValLeuLeuTrpGlyPheCysPheSerSe 1883 : GGGAAAAGGTGTGGGAACCCGCAGGAAGTTCTCCTCTGGGGATtctgtttctcctc : 1937 23 : oGlyLeuAlaGlyThrMetCysAlaSerArg{A} >>>> Target Intron 1 : 33 ...||| ::: ||| {!} 8621 bp rSerLeuProAspSerSerSerLeuSerSer{A}++ 1938 : ttccctccctgattcctcttctctctcttca{g}gt.................... : 1973 34 : >>>> {sp}AspTrpArgCysAlaArgSerMetHisGluPheSerAlaLysAsp : 48 { !} ! ! ! :!!!:! ! ||| !|||!.!!.!|||||| ++{la}ProLeuLeuGlySerGlnLeuAlaHisLeuPheLysGlyLysAsp 1974 : .....ag{ct}cctCTTCTTGGGTCTCAACTTGCACACTTGTTCAAGGGAAAGGAC : 10636 49 : IleAspGlyHisMetValAsnLeuAspLys >>>> Target Intron 2 >>> : 59 ||| |||!!.! !||| !:!! !! ! 17783 bp IleTrpGlyGlnLysValProIleCysIle++ 10637 : ATCTGGGGTCAGAAGGTCCCTATCTGTATAgt........................ : 10671 60 : > TyrArgGlyPheValCys{Il} >>>> Target Intron 3 >>>> {e : 65 !::|||||| ! !|||{ } 118109 bp { ++TrpArgGlyVal***Cys{Gl}++ ++{y 10672 : .agTGGCGAGGAGTGTGATGC{GG}gt.........................ag{G : 146581 66 : }ValThrAsnValAla{Se} >>>> Target Intron 4 >>>> {r}Gln : 73 } !! !||| !|||{||} 24462 bp {|}||| }GlnArgAsnProAla{Se}++ ++{r}Gln+ 146582 : }CAGAGGAACCCAGCC{AG}gt.........................ag{C}CAGg : 171065 74 : >>>> Target Intron 5 >>>> {U} >>>> Target Intron 6 >> : 73 85207 bp { } 16488 bp + ++{G}++ 171066 : t.........................ag{G}gt....................... : 256275 74 : >> {nk}GlyLysThrGluVal{A} >>>> Target Intron 7 >>>> { : 79 { }|||:!!..! !|||{.} 167261 bp { ++{ly}GlyGluValThrVal{G}++ ++{ 256276 : ..ag{GA}GGTGAGGTTACAGTA{G}gt.........................ag{ : 440040 80 : sn}TyrThrGlnLeuValAspLeuHis >>>> Target Intron 8 >>>> : 88 !.}:!!|||||| !!.!||| !||| 16192 bp lu}HisThrGlnGlyAlaAspThrHis-+ ++ 440041 : AA}CACACACAGGGAGCAGACACCCATct.........................ag : 456256 89 : AlaArgTyrAlaGlu{C} >>>> Target Intron 9 >>>> {ys}GlyLe : 95 ||||||! !!.! {|} 98361 bp {||}||| AlaArgSerValPro{C}++ ++{ys}GlyAr 456257 : GCGAGGTCCGTGCCC{T}gt.........................ag{GT}GGGAG : 554640 96 : uArg{Il} >>>> Target Intron 10 >>>> {e}LeuAlaPheProCys : 102 !|||{ } 19452 bp { }||| |||||| gArg{Gl}++ ++{n}LeuProPhePro*** 554641 : AAGA{CA}gt..........................ag{g}ttgccatttccatag : 574113 103 : AsnGln{P} >>>> Target Intron 11 >>>> {he}GlyLysGlnGluP : 110 .!.|||{!} 27337 bp {::} ...| GlnGln{T}+- ++{rp}AspLeuProAsnP 574114 : CAACAA{T}gg..........................ag{gg}gatcttcccaatc : 601474 111 : ro{G} >>>> Target Intron 12 >>>> {ly}SerAsnGluGluIleLy : 117 ||{|} 53360 bp {||}!!!!:!:!!:!!||||| ro{G}++ -+{ly}ThrSerLysGlnIleLy 601475 : ca{g}gt..........................gg{GA}ACCAGTAAACAAATAAA : 654855 118 : sGluPheAlaAlaGlyTyrAsnValLys >>>> Target Intron 13 >>>> : 127 | !|||!.!.!!!.!:!!...||| ! 11773 bp s***PheGlyThrAlaHisGlyValPro++ 654856 : GTGATTTGGGACAGCGCATGGGGTCCCAgt.......................... : 654887 128 : >>>> Target Intron 14 >>>> PheAspMetPheSer{Ly} >>> : 132 197115 bp .!. ! .!. !|||{!:} ++++ ++IleLysPheAsnSer{Ar}++ 654888 : aggt..........................agATAAAATTCAATTCC{AG}gt... : 863792 133 : > Target Intron 15 >>>> {s}IleCysValAsnGlyAspAsp{A} >> : 140 20512 bp {!} |||:!!|||.!!..!|||{|} ++{g}CysCysIleAsnSerSerAsp{A}-+ 863793 : .......................ag{a}tgcTGCATAAATAGTTCTGAT{G}at.. : 884327 141 : >> Target Intron 16 >>>> {la}HisProLeuTrpLysTrpMet{L} : 148 44232 bp {||}...|||! !|||||| { } ++{la}ArgProSerTrpLysProAla{T}++ 884328 : ........................ag{ct}agGCCATCATGGAAGCCTGCA{T}gt : 928581 149 : >>>> Target Intron 17 >>>> {ys}IleGlnProLysGlyLysGlyIle : 156 31004 bp { !}|||||||||:::... ...::: ++{rp}IleGlnProArgSerProAlaLeu 928582 : ..........................ag{gg}atccaacccaggtctcccgcattg : 959608 157 : LeuGlyAsnAlaIleLys{T} >>>> Target Intron 18 >>>> {rp}A : 164 ...::::::::: {|} 85934 bp {||}! GlnAlaAspSerLeuPro{T}-+ ++{rp}S 959609 : caggcagattctttacca{t}ct..........................ag{GG}A : 1045566 165 : snPheThrLysPheLeuIleAsp >>>> Target Intron 19 >>>> > : 172 :!!:: ! !|||:!!||| 66558 bp erTrpLeuLeuPheValIleArg++ ++++ 1045567 : GCTGGCTTTTATTCGTGATTAGGgt..........................aggt. : 1112150 173 : >>> Target Intron 20 >>>> LysAsnGlyCysValValLysArgTyrGl : 181 13243 bp !|||||| !:!! ! !! ! || ++AlaCysGlyCysProLeuTyrTrpLysGl 1112151 : .........................agGCCTGTGGCTGCCCGCTCTACTGGAAGGG : 1125418 182 : yProMet : 183 ||||:!: yProLeu 1125419 : ACCGCTC : 1125426 vulgar: SPP00000007_1.0 3 183 . NIVO01030317.1:subseq(555646,1182752) 1882 1125426 + 106 M 29 87 S 0 1 5 0 2 I 0 8617 3 0 2 S 1 2 M 25 75 5 0 2 I 0 17779 3 0 2 M 6 18 S 0 2 5 0 2 I 0 118105 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 24458 3 0 2 S 1 1 M 1 3 5 0 2 I 0 85203 3 0 2 S 0 1 5 0 2 I 0 16484 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 167257 3 0 2 S 1 2 M 8 24 5 0 2 I 0 16188 3 0 2 M 5 15 S 0 1 5 0 2 I 0 98357 3 0 2 S 1 2 M 3 9 S 0 2 5 0 2 I 0 19448 3 0 2 S 1 1 M 7 21 S 0 1 5 0 2 I 0 27333 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 53356 3 0 2 S 1 2 M 15 45 5 0 2 I 0 11769 3 0 2 5 0 2 I 0 197111 3 0 2 M 5 15 S 0 2 5 0 2 I 0 20508 3 0 2 S 1 1 M 7 21 S 0 1 5 0 2 I 0 44228 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 31000 3 0 2 S 1 2 M 14 42 S 0 1 5 0 2 I 0 85930 3 0 2 S 1 2 M 8 24 5 0 2 I 0 66554 3 0 2 5 0 2 I 0 13239 3 0 2 M 12 36 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 1883 1125426 106 + . gene_id 33 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1883 1970 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1883 1970 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1971 1972 . + . intron_id 1 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1971 10591 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 10590 10591 . + . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 10592 10668 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 10592 10668 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 10669 10670 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 10669 28451 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 28450 28451 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 28452 28471 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 28452 28471 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 28472 28473 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 28472 146580 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 146579 146580 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 146581 146598 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 146581 146598 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 146599 146600 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 146599 171060 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 171059 171060 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 171061 171064 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 171061 171064 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 171065 171066 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 171065 256271 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 256270 256271 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 256272 256272 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 256272 256272 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 256273 256274 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 256273 272760 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 272759 272760 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 272761 272778 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 272761 272778 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 272779 272780 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 272779 440039 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 440038 440039 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 440040 440065 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 440040 440065 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 440066 440067 . + . intron_id 8 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 440066 456257 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 456256 456257 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 456258 456273 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 456258 456273 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 456274 456275 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 456274 554634 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 554633 554634 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 554635 554647 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 554635 554647 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 554648 554649 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 554648 574099 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 574098 574099 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 574100 574122 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 574100 574122 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 574123 574124 . + . intron_id 11 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 574123 601459 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 654837 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 654836 654837 . + . intron_id 11 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 654838 654884 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 654838 654884 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 654885 654886 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 654885 666657 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 863772 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 863771 863772 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 863773 863789 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 863773 863789 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 863790 863791 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 863790 884301 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 884300 884301 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 884302 884324 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 884302 884324 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 884325 884326 . + . intron_id 16 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 884325 928556 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 928555 928556 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 928557 928580 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 928557 928580 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 928581 928582 . + . intron_id 17 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 928581 959584 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 959583 959584 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 959585 959629 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 959585 959629 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 959630 959631 . + . intron_id 18 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 959630 1045563 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1045562 1045563 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1045564 1045589 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1045564 1045589 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1045590 1045591 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1045590 1112147 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1125390 . + . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1125389 1125390 . + . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1125391 1125426 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1125391 1125426 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 1883 1125426 106 + . alignment_id 33 ; Query SPP00000007_1.0 ; Align 1883 4 87 ; Align 10594 34 75 ; Align 28452 59 18 ; Align 146582 66 15 ; Align 171062 72 3 ; Align 272763 74 15 ; Align 440042 80 24 ; Align 456258 88 15 ; Align 554637 94 9 ; Align 574101 98 21 ; Align 601462 106 15 ; Align 654840 112 45 ; Align 863773 127 15 ; Align 884303 133 21 ; Align 928559 141 21 ; Align 959587 149 42 ; Align 1045566 164 24 ; Align 1125391 172 36 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 103 Query range: 15 -> 195 Target range: 33855 -> 1047228 16 : CysGlyAlaLeuAla{A} >>>> Target Intron 1 >>>> {la}ProGl : 23 ||||||.!!!!!|||{|} 82079 bp {||}||| CysGlyThrPheAla{A}++ ++{la}ProLe 33856 : TGCGGCACCTTCGCA{G}gt.........................ag{cc}cccct : 115956 24 : yLeuAlaGlyThrMetCys{A} >>>> Target Intron 2 >>>> {la}S : 31 |||:!!||| !.!. !{:} 6260 bp {:!}! uLeuSerGlyGlnPheAsp{S}++ ++{er}T 115957 : gCTCTCTGGGCAGTTTGAC{A}gt.........................ag{GC}A : 122240 32 : erArgAspAspTrpArgCysAla >>>> Target Intron 3 >>>> ArgS : 40 !! ! !!!:||||||||| ! 8844 bp ! !! hrTyrProGluTrpArgCysTrp++ ++ProA 122241 : CATACCCTGAGTGGAGGTGCTGGgt.........................agCCCA : 131111 41 : erMetHisGluPheSerAlaLysAspIleAsp{G} >>>> Target Intron : 51 !! |||...|||!!!|||..! :!!!!:{|} 25717 bp rgAlaHisSerPheThrAlaSerTrpLeuGlu{G}++ 131112 : GGGCCCACAGCTTCACAGCATCGTGGCTAGAG{G}gt................... : 131147 52 : 4 >>>> {ly}HisMetValAsnLeuAspLysTyrArg{G} >>>> Target : 61 {||}:!!:!! !!!!.:!!:!! !:!|||{.} 122 ++{ly}TyrValPheLysValAsnGlyPheArg{S}++ 131148 : ......ag{GT}TATGTGTTTAAAGTTAATGGTTTCAGA{T}gt............ : 156894 62 : Intron 5 >>>> {ly}PheValCysIleValThrAsnValAlaSerGlnUnkG : 74 73 bp {.!} ||| ! :!:!.! !!....!!.!:!!! !...| ++{er}GlyValArgValAlaGlyThrThrGlyAlaLeuAlaG 156895 : .............ag{CC}GGAGTCAGGGTGGCGGGTACGACCGGAGCTCTGGCGG : 169203 75 : lyLysThrGluValAsnTyrThrGlnLeu >>>> Target Intron 6 >>>> : 84 || ||||||:!! ! ||| :!! 37288 bp lyProThrGluIleAlaThrThrValMet++ 169204 : GTCCTACAGAGATAGCTACGACAGTTATGgt......................... : 169235 85 : ValAspLeuHis{Al} >>>> Target Intron 7 >>>> {a}ArgTyr : 90 ||| :!!|||{!.} 63672 bp {!} ! +-Val***ValHis{Gl}++ ++{y}SerThr 169236 : acGTGTGAGTCCAC{GG}gt.........................ag{G}TCTACG : 270211 91 : AlaGluCysGlyLeuArgIleLeuAlaPheProCysAsn{G} >>>> Target : 104 .!!..!||| ! !! ! !:!!:!!!:!|||!! ...{ } 641 ThrArgCysGlnSerProAlaMetSerTyrProTrpGly{G}++ 270212 : ACCAGATGCCAGAGCCCCGCTATGTCGTATCCATGGGGA{G}gt............ : 270256 105 : Intron 8 >>>> {ln}PheGlyLysGlnGluProGlySerAsnGluGluIleL : 117 30 bp { }!:: ! !!::! !||||||:!!.....! :!: ++{ly}TrpProAlaArgGlyProGlyAlaGlyArgProValC 270257 : .............ag{GC}TGGCCAGCACGCGGGCCCGGGGCAGGGAGGCCCGTGT : 334422 118 : ysGluPheAlaAlaGlyTyrAsnValLysPhe >>>> Target Intron 9 > : 128 !!...!||| !!:!!!.! !:::!:! 115201 bp ysProLeuCysAlaArgHisThrSerArgTyr++ 334423 : GCCCTTTATGTGCCCGACACACTAGTCGCTACgt...................... : 334457 129 : >>> AspMetPheSerLysIleCysValAsn{Gl} >>>> Target Intron : 137 ! ! !!::::!!|||! ! {||} 12122 bp ++AlaTrpGlnProArgLeuCysGlyLeu{Gl}++ 334458 : ...agGCGTGGCAACCCCGCCTCTGCGGTTTG{GG}gt.................. : 449687 138 : 10 >>>> {y}AspAspAlaHis >>>> Target Intron 11 >>>> P : 142 {|}||| ...||| 111140 bp ++{y}AspGlyThrHis++ -+S 449688 : ........ag{g}gatgggacccacgt..........................tgt : 572960 143 : roLeuTrpLysTrpMet{Ly} >>>> Target Intron 12 >>>> {s}Il : 149 ||||||||| {!:} 81171 bp {!}|| erLeuTrpLysLeuHis{Ar}++ ++{g}Il 572961 : ctctCTGGAAACTACAC{AG}gt..........................ag{a}at : 654152 150 : eGlnProLysGlyLysGlyIleLeuGlyAsn >>>> Target Intron 13 > : 160 |:!! ! !.! !|||:!:|||..!!.. 12473 bp eGluLeuPheAlaLeuGlyLeuLeuSerArg++ 654153 : tgAGTTATTCGCCTTAGGGTTGTTATCCAGGgt....................... : 654187 161 : >>> >>>> Target Intron 14 >>>> AlaIleLysTrp >>>> Ta : 164 34766 bp |||:!! ++++ ++AlaValAlaGln++ 654188 : ...aggt..........................agGCGGTAGCTCAggt....... : 701438 165 : rget Intron 15 >>>> AsnPheThrLysPheLeuIleAspLysAsn{Gl} : 174 84756 bp |||||| |||...... ...:::{.!} ++AsnPheLeuProPhePhePhePheSerSer{Se}+ 701439 : ...................agaactttctcccttttttttttttttcttca{ag}g : 786224 175 : >>>> Target Intron 16 >>>> {y}CysValValLysArgTyrGlyPro : 182 167808 bp {!} :::||| ||| + ++{r}ValLeuValTrpArgIlePro*** 786225 : t..........................ag{t}gttcttgtctggagaatcccatga : 954054 183 : MetGluGluPro{L} >>>> Target Intron 17 >>>> {eu}ValIleG : 190 ||||||||||||{ } 93133 bp { !} ::: MetGluGluPro{A}++ ++{la}ProLeuP 954055 : atggaggagcct{g}gt..........................ag{cg}cccctcc : 1047211 191 : luLysAspLeuProHis : 195 ||||||||| roThrArgLeuProHis 1047212 : caaccaggctgccacat : 1047228 vulgar: SPP00000007_1.0 15 195 . NIVO01030317.1:subseq(555646,1182752) 33855 1047228 + 103 M 5 15 S 0 1 5 0 2 I 0 82075 3 0 2 S 1 2 M 8 24 S 0 1 5 0 2 I 0 6256 3 0 2 S 1 2 M 8 24 5 0 2 I 0 8840 3 0 2 M 12 36 S 0 1 5 0 2 I 0 25713 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 12269 3 0 2 S 1 2 M 22 66 5 0 2 I 0 37284 3 0 2 M 4 12 S 0 2 5 0 2 I 0 63668 3 0 2 S 1 1 M 15 45 S 0 1 5 0 2 I 0 64126 3 0 2 S 1 2 M 23 69 5 0 2 I 0 115197 3 0 2 M 9 27 S 0 2 5 0 2 I 0 12118 3 0 2 S 1 1 M 4 12 5 0 2 I 0 111136 3 0 2 M 6 18 S 0 2 5 0 2 I 0 81167 3 0 2 S 1 1 M 11 33 5 0 2 I 0 12469 3 0 2 5 0 2 I 0 34762 3 0 2 M 4 12 5 0 2 I 0 84752 3 0 2 M 10 30 S 0 2 5 0 2 I 0 167804 3 0 2 S 1 1 M 12 36 S 0 1 5 0 2 I 0 93129 3 0 2 S 1 2 M 8 24 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 33856 1047228 103 + . gene_id 34 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 33856 33871 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 33856 33871 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 33872 33873 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 33872 115950 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 115949 115950 . + . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 115951 115977 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 115951 115977 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 115978 115979 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 115978 122237 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 122236 122237 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 122238 122263 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 122238 122263 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 122264 122265 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 122264 131107 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 131106 131107 . + . intron_id 2 ; splice_site "aG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 131108 131144 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 131108 131144 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 131145 131146 . + . intron_id 4 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 131145 156861 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 156860 156861 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 156862 156891 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 156862 156891 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 156892 156893 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 156892 169164 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 169163 169164 . + . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 169165 169232 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 169165 169232 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 169233 169234 . + . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 169233 206520 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 206519 206520 . + . intron_id 5 ; splice_site "AC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 206521 206534 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 206521 206534 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 206535 206536 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 206535 270206 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 270205 270206 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 270207 270253 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 270207 270253 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 270254 270255 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 270254 334383 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 334382 334383 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 334384 334454 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 334384 334454 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 334455 334456 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 334455 449655 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 449654 449655 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 449656 449684 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 449656 449684 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 449685 449686 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 449685 461806 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 461805 461806 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 461807 461819 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 461807 461819 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 461820 461821 . + . intron_id 11 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 461820 572959 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 572958 572959 . + . intron_id 10 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 572960 572979 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 572960 572979 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 572980 572981 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 572980 654150 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 654149 654150 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 654151 654184 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 654151 654184 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 654185 654186 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 654185 666657 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 701423 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 701422 701423 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 701424 701435 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 701424 701435 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 701436 701437 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 701436 786191 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 786190 786191 . + . intron_id 14 ; splice_site "Ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 786192 786223 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 786192 786223 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 786224 786225 . + . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 786224 954031 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 954030 954031 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 954032 954069 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 954032 954069 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 954070 954071 . + . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 954070 1047202 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1047201 1047202 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1047203 1047228 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1047203 1047228 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 33856 1047228 103 + . alignment_id 34 ; Query SPP00000007_1.0 ; Align 33856 16 15 ; Align 115953 22 24 ; Align 122240 31 24 ; Align 131108 39 36 ; Align 156864 52 27 ; Align 169167 62 66 ; Align 206521 84 12 ; Align 270208 89 45 ; Align 334386 105 69 ; Align 449656 128 27 ; Align 461808 138 12 ; Align 572960 142 18 ; Align 654152 149 33 ; Align 701424 160 12 ; Align 786192 164 30 ; Align 954033 175 36 ; Align 1047205 188 24 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 103 Query range: 0 -> 162 Target range: 270239 -> 1163754 1 : MetSerLeuGly{Ar} >>>> Target Intron 1 >>>> {g}LeuCys{A : 8 :!:|||:!!|||{||} 4505 bp {|}:!!|||{| ValSerMetGly{Ar}++ ++{g}IleCys{A 270240 : GTATCCATGGGG{AG}gt.........................ag{G}ATCTGC{A : 274766 9 : r} >>>> Target Intron 2 >>>> {g}LeuLeuLysProAlaLeuLeu{ : 16 |} 131333 bp {|}:::|||::: |||{ r}++ ++{g}IleLeuGlnAlaArgThrLeu{ 274767 : G}gt.........................ag{g}attctccaggcaagaacactg{ : 406123 17 : Cy} >>>> Target Intron 3 >>>> {s}GlyAlaLeuAlaAlaProGly : 23 } 28863 bp { }||| !! !|||..!! !||| Ly}++ ++{s}GlyHisGlnAlaCysArgGly 406124 : aa}gt.........................ag{A}GGTCATCAAGCTTGCCGGGGA : 435007 24 : LeuAlaGlyThrMetCysAlaSerArgAspAspTrpArgCysAla{Ar} >>>> : 39 ||| !!! ! ! ||| !|||! ! ! ! !||||||{||} LeuProAspArgArgThrAlaValArgAlaGlyProLeuCysAla{Ar}++ 435008 : CTTCCAGACCGACGCACAGCCGTCAGAGCGGGTCCACTTTGTGCC{AG}gt..... : 435059 40 : Target Intron 4 >>>> {g}SerMetHis{Gl} >>>> Target Intr : 43 137911 bp {|}!.!:!:|||{..} 28480 b -+{g}LysLeuHis{Ar}++ 435060 : ....................tg{G}AAACTACAC{AG}gt................ : 572982 44 : on 5 >>>> {u}PheSerAlaLysAsp{Il} >>>> Target Intron 6 : 49 p {!} |||::::::...{! } 155703 bp ++{g}GlySerSerGlnSer{Ar}++ 572983 : .........ag{g}ggatcttcccaatcc{ag}gt..................... : 601480 50 : >>>> {e}AspGlyHisMetValAsnLeuAspLysTyrArgGly{Ph} >>>> : 62 { }|||||| ||| |||... |||{ } ++{g}AspGlyThrArgValPhe***AspSerCysPheGly{Se}++ 601481 : ....ag{g}gatggaacccgggtcttctgagactcctgctttggc{ag}gt..... : 757222 63 : Target Intron 7 >>>> {e}ValCysIleValThr{A} >>>> Target : 68 109048 bp {!}::: ::: :::{!} 24 ++{r}IleArgValPheSer{A}++ 757223 : ....................ag{c}atcagggtcttttca{a}gt........... : 866287 69 : Intron 8 >>>> {sn}ValAlaSerGlnUnkGlyLysThrGluValAsnTyr : 80 01 bp {..} ... ||| ! ! |||:::||| ++{rg}AspLeuProAspArgGlyValGluThrValSerTyr 866288 : ..............ag{ga}gatcttcccgaccgaggggtGGAAactgtctcttac : 868721 81 : ThrGlnLeuValAsp{Le} >>>> Target Intron 9 >>>> {u}HisAl : 88 ...... ... { } 111523 bp {!}...|| ValSerCysThrGly{Ar}++ ++{g}ArgAl 868722 : gtctcctgcactggc{ag}gt.........................ag{g}cgggc : 980268 89 : aArgTyrAlaGluCysGlyLeu >>>> Target Intron 10 >>>> >> : 96 | ||| |||||| ||| 31085 bp aLeuTyrHisGluCysGlnLeu+- ++++ 980269 : tctttaccacgagtgccagctggg..........................aggt.. : 1011379 97 : >> Target Intron 11 >>>> ArgIleLeuAlaPhePro >>>> Targe : 102 79655 bp !!:!::!!||| !||| ++GlyLeuValAlaGlyPro++ 1011380 : ........................agGGTCTGGTCGCCGGCCCCgt.......... : 1091052 103 : t Intron 12 >>>> CysAsnGlnPheGlyLysGlnGluProGlySerAsnGl : 114 8257 bp ||| ! !||||||:!!:!! !! !!:! :! ++ThrAsnThrThrGlyLysLysGlnAlaProAsnProLy 1091053 : ................agACAAACACGACTGGAAAAAAGCAAGCCCCAAACCCGAA : 1099343 115 : uGluIleLysGluPheAlaAla{Gl} >>>> Target Intron 13 >>>> : 122 !...|||:::||| ... {||} 12411 bp sSerIleArgGluAlaGlyArg{Gl}++ ++ 1099344 : GTCCATtagagaggcaggaaga{gg}gt..........................ag : 1111778 123 : {y}TyrAsnValLysPheAspMetPheSer{Ly} >>>> Target Intron 1 : 132 {|}|||||| ! !! |||!!:!:!!!!{ } 324 bp {y}TyrAsnProLeuSerAspIleTyrThr{Va}++ 1111779 : {A}TACAATCCCTTATCGGATATATATACT{Gt}gt.................... : 1111812 133 : 4 >>>> {s}IleCysValAsn{G} >>>> Target Intron 15 >>>> : 137 { }:!!||| !!:::{|} 47405 bp ++{l}LeuCysPheSer{G}++ +- 1111813 : ......ag{C}CTCTGTTTCTCA{G}gt..........................aa : 1159551 138 : {ly}AspAspAlaHisProLeuTrpLysTrpMetLysIle{G} >>>> Target : 150 {||}||| ||| !||| !|||:!! !! :!:{:} 4 {ly}AspProAlaSerProGluTrpGlnArgGlyAlaVal{G}++ 1159552 : {GG}GACCCAGCCTCTCCTGAGTGGCAGCGGGGAGCCGTG{G}gt........... : 1159594 151 : Intron 16 >>>> {ln}ProLysGlyLysGlyIleLeuGlyAsnAlaIleLy : 162 125 bp {!!} :::|||||| ||||||......|||::::: ++{lu}SerArgGlyLysArgIleLeuAlaGlyAlaValAr 1159595 : ...............ag{aa}tcccgtggaaagaggatcctggcgggtgcagtccg : 1163752 163 : s : 162 : g 1163753 : t : 1163754 vulgar: SPP00000007_1.0 0 162 . NIVO01030317.1:subseq(555646,1182752) 270239 1163754 + 103 M 4 12 S 0 2 5 0 2 I 0 4501 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 131329 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 28859 3 0 2 S 1 1 M 22 66 S 0 2 5 0 2 I 0 137907 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 28476 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 155699 3 0 2 S 1 1 M 12 36 S 0 2 5 0 2 I 0 109044 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 2397 3 0 2 S 1 2 M 17 51 S 0 2 5 0 2 I 0 111519 3 0 2 S 1 1 M 9 27 5 0 2 I 0 31081 3 0 2 5 0 2 I 0 79651 3 0 2 M 6 18 5 0 2 I 0 8253 3 0 2 M 20 60 S 0 2 5 0 2 I 0 12407 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 320 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 47401 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 4121 3 0 2 S 1 2 M 12 36 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 270240 1163754 103 + . gene_id 35 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 270240 270253 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 270240 270253 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 270254 270255 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 270254 274758 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 274757 274758 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 274759 274767 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 274759 274767 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 274768 274769 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 274768 406100 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 406099 406100 . + . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 406101 406124 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 406101 406124 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 406125 406126 . + . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 406125 434987 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 434986 434987 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 434988 435056 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 434988 435056 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 435057 435058 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 435057 572967 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 572966 572967 . + . intron_id 3 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 572968 572979 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 572968 572979 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 572980 572981 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 572980 601459 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 757180 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 757179 757180 . + . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 757181 757219 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 757181 757219 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 757220 757221 . + . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 757220 866267 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 866266 866267 . + . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 866268 866284 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 866268 866284 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 866285 866286 . + . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 866285 868685 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 868684 868685 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 868686 868740 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 868686 868740 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 868741 868742 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 868741 980263 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 980262 980263 . + . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 980264 980291 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 980264 980291 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 980292 980293 . + . intron_id 10 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 980292 1011376 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1091031 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1091030 1091031 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1091032 1091049 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1091032 1091049 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1091050 1091051 . + . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1091050 1099306 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1099305 1099306 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1099307 1099368 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1099307 1099368 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1099369 1099370 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1099369 1111779 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1111778 1111779 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1111780 1111809 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1111780 1111809 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1111810 1111811 . + . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1111810 1112133 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112132 1112133 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1112134 1112147 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1112134 1112147 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1159552 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1159551 1159552 . + . intron_id 14 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1159553 1159591 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1159553 1159591 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1159592 1159593 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1159592 1163716 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1163715 1163716 . + . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1163717 1163754 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1163717 1163754 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 270240 1163754 103 + . alignment_id 35 ; Query SPP00000007_1.0 ; Align 270240 1 12 ; Align 274760 6 6 ; Align 406102 9 21 ; Align 434989 17 66 ; Align 572969 40 9 ; Align 601461 44 15 ; Align 757182 50 36 ; Align 866269 63 15 ; Align 868688 69 51 ; Align 980265 87 27 ; Align 1091032 96 18 ; Align 1099307 102 60 ; Align 1111781 123 27 ; Align 1112135 133 12 ; Align 1159555 138 36 ; Align 1163719 151 36 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 102 Query range: 0 -> 158 Target range: 9097 -> 1124472 1 : MetSerLeuGlyArgLeuCysArgLeuLeuLys >>>> Target Intron 1 : 12 :!:||| !!.!! !||| ! !:!!|||||| 25780 bp LeuSerThrAlaLeuLeuValLeuValLeuLys++ 9098 : CTCAGCACCGCGCTGCTTGTGCTGGTGCTCAAGgt..................... : 9133 13 : >>>> ProAlaLeuLeuCysGlyAlaLeu >>>> Target Intron 2 >>> : 20 ||| ||||||||| 126955 bp ++ArgArgLeuArgThrGlyAlaLeu++ 9134 : ....agaggaggctgaggacGGGGGCCTTGgt........................ : 34937 21 : > AlaAlaProGlyLeuAlaGlyThrMet >>>> Target Intron 3 >>> : 29 ||| !|||.!! !|||!.!||| ! 92472 bp ++AlaGlnProSerLysAlaAlaThrGly++ 34938 : .agGCCCAGCCGAGCAAAGCGGCCACTGGGgt........................ : 161919 30 : > CysAla{Se} >>>> Target Intron 4 >>>> {r}ArgAspAspTr : 35 |||:!!{!!} 75774 bp {!}!.!||| || ++CysSer{Ar}++ ++{g}AsnAspMetTr 161920 : .agTGCTCC{AG}gt.........................ag{A}AATGATATGTG : 330181 36 : pArgCysAlaArgSerMetHisGluPheSerAla{L} >>>> Target Intro : 47 |!:!||| !..!!.!!!:|||:!!.!!..! !{:} 132412 bp pLysCysIleGluLysIleHisLysIleGlnLys{A}++ 330182 : GAAATGCATTGAAAAAATTCACAAGATCCAGAAA{c}gt................. : 330220 48 : n 5 >>>> {ys}AspIleAspGly{H} >>>> Target Intron 6 >>>> : 52 {:!} ::: |||{:} 72240 bp ++{rg}ValMetTyrGly{T}++ 330221 : ........ag{ga}gtcatgtatgga{t}gt......................... : 462647 53 : {is}MetValAsnLeuAspLysTyrArgGlyPheValCys{Il} >>>> Tar : 65 {!!}!!:|||::::!! !! ! ! ! !.!.:!!|||{!!} ++{yr}IleValSerIleHisValLeuThrHisIleLeuCys{Me}++ 462648 : ag{AC}ATAGTATCAATACACGTACTAACCCATATACTGTGT{AT}gt........ : 534927 66 : get Intron 7 >>>> {e}ValThrAsnValAla{Se} >>>> Target I : 71 66535 bp {:} :::::: :::{||} 8676 ++{t}GlySerSerGlnSer{Se}++ 534928 : .................ag{g}ggatcttcccaatcc{ag}gt............. : 601480 72 : ntron 8 >>>> {r}GlnUnkGlyLysThrGluValAsnTyrThr{G} >>>> : 82 8 bp {|} :::|||...||| !|||{:} ++{r}ValTrpAspArgThrArgValProArgThr{G}++ 601481 : ............ag{c}gtgtgggatcgaacccgcgtccccCGCAcc{g}gt.... : 688280 83 : Target Intron 9 >>>> {ln}LeuValAspLeuHisAlaArgTyrAla{G : 92 11098 bp {!!}|||...... ||||||... |||{! ++{lu}LeuAlaGlnThrHisAlaHisGlnAla{A 688281 : .....................ag{ag}cttgctcaaactcacgcccatcaagct{g : 699405 93 : } >>>> Target Intron 10 >>>> {lu}Cys{Gl} >>>> Target : 94 } 144772 bp { }|||{||} 220 }++ -+{la}Cys{Gl}++ 699406 : }gt..........................tg{CC}TGT{GG}gt............ : 844187 95 : Intron 11 >>>> {y}LeuArgIleLeuAla{P} >>>> Target Intro : 100 83 bp {|}:::|||:::...:::{ } 79721 bp ++{y}IleArgValPheSer{A}++ 844188 : ..............ag{c}atcagggtcttttca{a}gt................. : 866287 101 : n 12 >>>> {he}ProCysAsnGlnPheGlyLysGlnGluProGly >>>> T : 112 { }||| ! ||| ||| !... |||||| ++{rg}ProArgProGlnGlyGlyLeuSerIleProGly++ 866288 : .........ag{GA}CCCCGGCCACAAGGAGGTCTGTCCATTCCCGGGgt...... : 946043 113 : arget Intron 13 >>>> >>>> Target Intron 14 >>>> SerA : 113 65336 bp 15574 bp |||| ++++ ++SerA 946044 : ....................aggt..........................agtcca : 1026954 114 : snGluGluIleLysGluPheAlaAlaGlyTyrAsnValLysPheAspMetPhe{Se : 131 ||......||| .........||| ...::: ... :::{!! snSerHisIleProTyrMetThrThrGlyLysThrIleAlaLeuThrArgTrp{Th 1026955 : actctcacattccatatatgactactggaaaaaccatagccttgactagatgg{ac : 1027008 132 : } >>>> Target Intron 15 >>>> {r}Lys >>>> Target Intro : 133 } 41075 bp {!}||| 26650 bp }++ ++{r}Lys+- 1027009 : }gt..........................ag{t}aaggg................. : 1068091 134 : n 16 >>>> IleCysVal >>>> Target Intron 17 >>>> AsnGly : 137 ! !|||..! 3000 bp ...... ++SerCysThr++ ++GlySer 1068092 : .........agAGCTGCACTgt..........................agggatcg : 1097751 138 : AspAspAlaHisProLeuTrpLys >>>> Target Intron 18 >>>> : 146 ::: ::: |||::: 14370 bp AsnLeuSerPheLeuHisTrpGln++ ++++ 1097752 : aacctgagtttcctacattggcaggt..........................aggt : 1112148 147 : >>>> Target Intron 19 >>>> TrpMetLysIleGlnProLysGlyLysG : 155 12286 bp ::: :::||| .........| ++GlyIlePheLeuGlnGluSerAsnSerG 1112149 : ..........................aggggatcttcctccaggagtcgaactctg : 1124461 156 : lyIleLeuGly : 158 ||:::|||||| lyLeuLeuGly 1124462 : gtctcctgggA : 1124472 vulgar: SPP00000007_1.0 0 158 . NIVO01030317.1:subseq(555646,1182752) 9097 1124472 + 102 M 11 33 5 0 2 I 0 25776 3 0 2 M 8 24 5 0 2 I 0 126951 3 0 2 M 9 27 5 0 2 I 0 92468 3 0 2 M 2 6 S 0 2 5 0 2 I 0 75770 3 0 2 S 1 1 M 15 45 S 0 1 5 0 2 I 0 132408 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 72236 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 66531 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 86764 3 0 2 S 1 1 M 10 30 S 0 1 5 0 2 I 0 11094 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 144768 3 0 2 S 1 2 M 1 3 S 0 2 5 0 2 I 0 22079 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 79717 3 0 2 S 1 2 M 11 33 5 0 2 I 0 65332 3 0 2 5 0 2 I 0 15570 3 0 2 M 19 57 S 0 2 5 0 2 I 0 41071 3 0 2 S 1 1 M 1 3 5 0 2 I 0 26646 3 0 2 M 3 9 5 0 2 I 0 2996 3 0 2 M 10 30 5 0 2 I 0 14366 3 0 2 5 0 2 I 0 12282 3 0 2 M 13 39 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 9098 1124472 102 + . gene_id 36 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 9098 9130 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 9098 9130 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 9131 9132 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 9131 34910 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 34909 34910 . + . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 34911 34934 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 34911 34934 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 34935 34936 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 34935 161889 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 161888 161889 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 161890 161916 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 161890 161916 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 161917 161918 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 161917 254388 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 254387 254388 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 254389 254396 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 254389 254396 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 254397 254398 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 254397 330170 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 330169 330170 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 330171 330217 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 330171 330217 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 330218 330219 . + . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 330218 462629 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462628 462629 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462630 462644 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462630 462644 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462645 462646 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462645 534884 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 534883 534884 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 534885 534924 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 534885 534924 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 534925 534926 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 534925 601459 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 688245 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 688244 688245 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 688246 688277 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 688246 688277 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 688278 688279 . + . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 688278 699375 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 699374 699375 . + . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 699376 699405 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 699376 699405 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 699406 699407 . + . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 699406 844177 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 844176 844177 . + . intron_id 9 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 844178 844184 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 844178 844184 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 844185 844186 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 844185 866267 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 866266 866267 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 866268 866284 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 866268 866284 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 866285 866286 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 866285 946005 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 946004 946005 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 946006 946040 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 946006 946040 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 946041 946042 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 946041 1011376 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1026950 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1026949 1026950 . + . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1026951 1027009 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1026951 1027009 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1027010 1027011 . + . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1027010 1068084 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1068083 1068084 . + . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1068085 1068088 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1068085 1068088 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1068089 1068090 . + . intron_id 16 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1068089 1094738 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1094737 1094738 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1094739 1094747 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1094739 1094747 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1094748 1094749 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1094748 1097747 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1097746 1097747 . + . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1097748 1097777 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1097748 1097777 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1097778 1097779 . + . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1097778 1112147 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1124433 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1124432 1124433 . + . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1124434 1124472 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1124434 1124472 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 9098 1124472 102 + . alignment_id 36 ; Query SPP00000007_1.0 ; Align 9098 1 33 ; Align 34911 12 24 ; Align 161890 20 27 ; Align 254389 29 6 ; Align 330172 32 45 ; Align 462632 48 12 ; Align 534887 53 36 ; Align 601461 66 15 ; Align 688247 72 30 ; Align 699378 83 27 ; Align 844180 93 3 ; Align 866269 95 15 ; Align 946008 101 33 ; Align 1026951 112 57 ; Align 1068086 132 3 ; Align 1094739 133 9 ; Align 1097748 136 30 ; Align 1124434 146 39 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 101 Query range: 3 -> 164 Target range: 312038 -> 1180046 4 : GlyArgLeuCys{Ar} >>>> Target Intron 1 >>>> {g}LeuLeuLy : 11 ||||||:!!|||{||} 47234 bp {|}|||:::.. GlyArgValCys{Ar}++ ++{g}LeuValSe 312039 : GGGAGGGTCTGC{AG}gt.........................ag{G}ctcgtgtc : 359294 12 : sProAlaLeu >>>> Target Intron 2 >>>> LeuCysGlyAlaLeuAl : 20 .||||||||| 7271 bp ||| !|||||| !!. rProAlaLeu+- ++LeuLeuGlyAlaAlaGl 359295 : tcctgccctggc.........................agtTGCTCGGGGCTGCAGG : 366592 21 : aAlaProGlyLeu >>>> Target Intron 3 >>>> AlaGlyThrMetCy : 29 !!.!||||||||| 32881 bp !.!!.!.!! yValProGlyLeu++ ++GlyAlaAlaArgMe 366593 : TGTCCCTGGGCTTgt.........................agGGAGCGGCCCGAAT : 399500 30 : sAlaSerArgAsp{A} >>>> Target Intron 4 >>>> {sp}TrpArgC : 37 :!!.!!||||||{!} 98379 bp { }|||||| tSerGlyArgAsp{G}++ ++{ly}TrpArgA 399501 : GTCTGGGCGTGAC{G}gt.........................ag{GA}TGGAGGA : 497903 38 : ysAlaArg >>>> Target Intron 5 >>>> SerMetHisGluPheSer : 46 ! !.!||| 103548 bp ...::: ...... rgGlyArg++ ++GlyIlePheProIleGln+ 497904 : GGGGACGGgt.........................aggggatcttcccaatccagg : 601478 47 : >>>> Target Intron 6 >>>> >>>> Target Intron 7 >>>> : 46 65180 bp 44579 bp + ++++ + 601479 : t.........................aggt.........................a : 711235 47 : AlaLysAspIleAspGly{Hi} >>>> Target Intron 8 >>>> {s}M : 53 ! ! |||! !!:|||{..} 149255 bp {.}. +GlnAspAspArgGluGly{Ar}++ ++{g}G 711236 : gCAGGATGACAGGGAGGGA{AG}gt.........................ag{A}C : 860513 54 : etValAsnLeuAspLysTyrArgGly >>>> Target Intron 9 >>>> : 62 .!||||||||| ! ! |||!:! ! 150837 bp lnValAsnLeuLysAspTyrGlnGln++ +++ 860514 : AGGTCAACTTAAAAGATTATCAGCAGgt.........................agg : 1011377 63 : >>>> Target Intron 10 >>>> PheValCysIleValThrAsnValAla : 70 83168 bp |||:!!||||||!.!:!!|||:!! ! + ++PheLeuCysIleAlaSerAsnLeuArg 1011378 : t..........................agttCTTATGTATTGCATCCAATCTACGA : 1094569 71 : Ser >>>> Target Intron 11 >>>> GlnUnkGlyLysThrGluValAs : 79 !:! 3173 bp ...... ::: ::::: Asn++ ++GlySerAsnLeuSerPheLeuHi 1094570 : AATgt..........................agggatcgaacctgagtttcctaca : 1097769 80 : nTyrThr >>>> Target Intron 12 >>>> >>>> Target Intro : 82 :::: 14370 bp 14796 bp sTrpGln++ ++++ 1097770 : ttggcaggt..........................aggt................. : 1112150 83 : n 13 >>>> GlnLeuValAspLeuHisAlaArgTyrAlaGluCys >>>> Ta : 94 :!! !!.!!!:!!!|||:!!|||||| ! ++GluAlaAlaGluPheHisSerArgTyrIleThrThr++ 1112151 : .........agGAGGCGGCCGAGTTCCATTCCCGCTACATCACCACGgt....... : 1126982 95 : rget Intron 14 >>>> {G} >>>> Target Intron 15 >>>> {l : 94 9757 bp {|} 11431 bp {| ++{G}++ ++{l 1126983 : ...................ag{G}gt..........................ag{G : 1148169 95 : y}LeuArgIleLeuAla{P} >>>> Target Intron 16 >>>> {he}Pr : 101 |}:!!||| ! !!{ } 1122 bp { }|| y}IleArgGlyArgPro{G}++ ++{ly}Pr 1148170 : G}ATAAGGGGGAGACCA{G}gt..........................ag{GG}CC : 1149311 102 : oCysAsnGlnPheGlyLysGlnGluProGlySerAsnGluGluIleLysGluPheA : 120 ||||!..:!!.!! !! ||| !|||..!||| ! !... !::: ! | oCysArgGluLeuArgAlaGlnThrProSerSerLeuProSerHisArgProAlaA 1149312 : CTGCAGAGAACTCCGGGCTCAGACACCCTCCTCGCTCCCATCCCACCGTCCAGCAG : 1149368 121 : laAla{G} >>>> Target Intron 17 >>>> {ly}TyrAsnValLysPh : 127 || !{|} 10969 bp {||} !!:!||| ! laArg{G}++ ++{ly}ValSerValPhe** 1149369 : CCCGA{G}gt..........................ag{GG}GTCAGCGTCTTCTA : 1160358 128 : eAspMetPheSerLysIleCysValAsn{Gl} >>>> Target Intron 18 : 137 !!::!!! :!!|||:!!!:!{||} 18184 bp *LeuGlyTrpThrCysValCysLeuSer{Gl}++ 1160359 : ACTGGGGTGGACGTGTGTCTGTCTTAGT{GG}gt...................... : 1160392 138 : >>>> {y}AspAspAlaHisProLeuTrpLysTrpMet{L} >>>> Target : 148 {|} ! !!:||| ! !|||!!.!! ..!{ } 13 ++{y}LysGluAlaAlaGluThrTrpAsnCysGln{G}++ 1160393 : ....ag{A}AAAGAGGCCGCAGAGACATGGAACTGCCAG{G}gt............ : 1178608 149 : Intron 19 >>>> {ys}IleGlnProLysGlyLysGlyIleLeuGlyAsnAla : 160 91 bp { }|||:!! ! ! |||||| !.!!:!!! ! ! ! ++{ly}IleGluValHisGlyLysLeuPheIleAspCysGln 1178609 : ..............ag{GC}ATTGAGGTTCATGGAAAACTGTTCATTGATTGTCAG : 1180032 161 : IleLysTrpAsn : 164 :!!:!!|||||| LeuGluTrpAsn 1180033 : CTAGAGTGGAAT : 1180046 vulgar: SPP00000007_1.0 3 164 . NIVO01030317.1:subseq(555646,1182752) 312038 1180046 + 101 M 4 12 S 0 2 5 0 2 I 0 47230 3 0 2 S 1 1 M 6 18 5 0 2 I 0 7267 3 0 2 M 10 30 5 0 2 I 0 32877 3 0 2 M 9 27 S 0 1 5 0 2 I 0 98375 3 0 2 S 1 2 M 5 15 5 0 2 I 0 103544 3 0 2 M 6 18 5 0 2 I 0 65176 3 0 2 5 0 2 I 0 44575 3 0 2 M 6 18 S 0 2 5 0 2 I 0 149251 3 0 2 S 1 1 M 9 27 5 0 2 I 0 150833 3 0 2 5 0 2 I 0 83164 3 0 2 M 10 30 5 0 2 I 0 3169 3 0 2 M 10 30 5 0 2 I 0 14366 3 0 2 5 0 2 I 0 14792 3 0 2 M 12 36 5 0 2 I 0 9753 3 0 2 S 0 1 5 0 2 I 0 11427 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 1118 3 0 2 S 1 2 M 21 63 S 0 1 5 0 2 I 0 10965 3 0 2 S 1 2 M 14 42 S 0 2 5 0 2 I 0 18180 3 0 2 S 1 1 M 10 30 S 0 1 5 0 2 I 0 1387 3 0 2 S 1 2 M 16 48 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 312039 1180046 101 + . gene_id 37 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 312039 312052 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 312039 312052 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 312053 312054 . + . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 312053 359286 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 359285 359286 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 359287 359305 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 359287 359305 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 359306 359307 . + . intron_id 2 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 359306 366576 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 366575 366576 . + . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 366577 366606 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 366577 366606 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 366607 366608 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 366607 399487 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 399486 399487 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 399488 399515 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 399488 399515 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 399516 399517 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 399516 497894 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 497893 497894 . + . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 497895 497911 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 497895 497911 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 497912 497913 . + . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 497912 601459 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 601458 601459 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 601460 601477 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 601460 601477 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 601478 601479 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 601478 666657 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 711236 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 711235 711236 . + . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 711237 711256 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 711237 711256 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 711257 711258 . + . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 711257 860511 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 860510 860511 . + . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 860512 860539 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 860512 860539 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 860540 860541 . + . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 860540 1011376 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011375 1011376 . + . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011377 1011378 . + . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011377 1094544 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1094543 1094544 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1094545 1094574 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1094545 1094574 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1094575 1094576 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1094575 1097747 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1097746 1097747 . + . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1097748 1097777 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1097748 1097777 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1097778 1097779 . + . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1097778 1112147 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112146 1112147 . + . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112148 1112149 . + . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112148 1126943 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1126942 1126943 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1126944 1126979 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1126944 1126979 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1126980 1126981 . + . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1126980 1136736 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1136735 1136736 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1136737 1136737 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1136737 1136737 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1136738 1136739 . + . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1136738 1148168 . + . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1148167 1148168 . + . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1148169 1148186 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1148169 1148186 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1148187 1148188 . + . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1148187 1149308 . + . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1149307 1149308 . + . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1149309 1149374 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1149309 1149374 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1149375 1149376 . + . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1149375 1160343 . + . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1160342 1160343 . + . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1160344 1160389 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1160344 1160389 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1160390 1160391 . + . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1160390 1178573 . + . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1178572 1178573 . + . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1178574 1178605 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1178574 1178605 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1178606 1178607 . + . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1178606 1179996 . + . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1179995 1179996 . + . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1179997 1180046 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1179997 1180046 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 312039 1180046 101 + . alignment_id 37 ; Query SPP00000007_1.0 ; Align 312039 4 12 ; Align 359288 9 18 ; Align 366577 15 30 ; Align 399488 25 27 ; Align 497897 35 15 ; Align 601460 40 18 ; Align 711237 46 18 ; Align 860513 53 27 ; Align 1094545 62 30 ; Align 1097748 72 30 ; Align 1126944 82 36 ; Align 1148171 95 15 ; Align 1149311 101 63 ; Align 1160346 123 42 ; Align 1178575 138 30 ; Align 1179999 149 48 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence Model: protein2genome:local Raw score: 100 Query range: 7 -> 197 Target range: 570784 -> 1122045 8 : ArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAlaGl : 26 |||||| !|||! !||| !! |||:::::: ...|||||| !! ArgLeuAlaLysGlnAlaThrTrpValGlySerIleProGlyProGlyThrGlnAs 570785 : cgccttGCAAAGCAGGCCACGTgggtgggttccatccctggtccgggaacccAGGA : 570839 27 : yThrMetCysAla{Se} >>>> Target Intron 1 >>>> {r}ArgAsp : 34 !:!!||||||!.!{||} 33778 bp {|}|||||| pSerMetCysVal{Se}+- ++{r}ArgAsp+ 570840 : TTCCATGTGTGTC{AG}gg.........................ag{C}CGGGATg : 604641 35 : >>>> Target Intron 2 >>>> >>>> Target Intron 3 >>>> : 34 62017 bp 39084 bp + ++++ + 604642 : t.........................aggt.........................a : 705740 35 : AspTrpArg >>>> Target Intron 4 >>>> CysAlaArgSerMetHi : 42 !|||||| 22250 bp ||| |||::::::|| +ArgTrpArg++ ++CysArgArgThrLeuHi 705741 : gCGCTGGAGGgt.........................agtgcaggcggactcttca : 728016 43 : sGluPheSerAlaLys >>>> Target Intron 5 >>>> AspIleAspGl : 51 |:::... ::: 29147 bp ::: sGlnLeuHisLeuGln+- ++GlyTrpAsnPr 728017 : ccagctgcacctccaggg.........................agggatggaaccc : 757190 52 : yHisMetValAsnLeuAspLysTyrArg >>>> Target Intron 6 >>>> : 61 ::::::...||| :::::: 93930 bp oGlyLeuLeuArgLeuLeuLeuTrpGln++ + 757191 : gggtcttctgagactcctgctttggcaggt.........................a : 851148 62 : GlyPheValCysIleValThrAsnValAlaSerGlnUnkGlyLysThr{Gl} > : 77 ! !|||||||||.!!:!!|||:!!!.!:!! ||||||!.!{..} -LysArgCysCysIleValAlaHisValSerLysGluGlnGlyLysAsn{Ar}++ 851149 : aAAGAGATGCTGCATTGTAGCTCACGTTTCCAAGGAACAGGGAAAGAAT{AG}gt. : 851202 78 : >>> Target Intron 7 >>>> {u}ValAsnTyrThrGlnLeuValAspLeu : 86 131107 bp {!}|||!:!!:: ! !! ! !! !:!! ++{g}ValSerTrpCysValCysArgGlyVal 851203 : ........................ag{g}gTGAGCTGGTGCGTGTGCAGAGGTGTA : 982332 87 : HisAlaArg >>>> Target Intron 8 >>>> TyrAlaGluCysGlyLeu : 95 ||| !||| 5343 bp ||| ! !|||||| HisLeuArg++ ++LeuAlaLeuHisGlyLeu 982333 : CACCTAAGGgt.........................agttAGCTCTGCACGGTCTT : 987702 96 : ArgIleLeuAlaPheProCysAsnGln{Ph} >>>> Target Intron 9 >> : 105 !!||||||||| !! ! ! ... !{ } 47625 bp GlyIleLeuAlaGlyArgGlyArgGly{Ar}-+ 987703 : GGCATCCTTGCCGGCCGGGGGCGGGGG{AG}ct....................... : 987736 106 : >> {e}GlyLysGlnGluProGlySerAsnGluGluIleLysGluPheAlaAlaG : 122 { }|||......:::||| .........::::::...... |||...| ++{g}GlySerSerGlnProArgAspGlnSerGlnValSerSerThrAlaGlyG 987737 : ..ag{a}ggatcttcccaacccagggatcaatcccaggtttccagcactgcaggtg : 1035408 123 : lyTyrAsnVal{Ly} >>>> Target Intron 10 >>>> {s}PheAspMe : 129 ||::: ...{!:} 6692 bp {!}|||! :! lyPhePheThr{Ar}-+ ++{g}PheGlyLe 1035409 : gattcttcacc{ag}ct..........................ag{a}TTTGGGTT : 1042121 130 : tPheSerLys{I} >>>> Target Intron 11 >>>> {le}CysValAsn : 136 ! ! !:! !{ } 13267 bp { !} ..!... uValAsnLeu{G}++ ++{ly}LeuThrGly 1042122 : GGTAAATCTG{G}gt..........................ag{GT}CTAACTGGG : 1055409 137 : GlyAspAspAlaHisProLeuTrpLysTrpMetLysIleGlnProLys >>>> T : 153 ||| !! !! !||| !! !||| ||| ...:!!! |||:!! GlyHisHisLeuHisPheProTrpProTrpSerSerLeuProProGln+- 1055410 : GGTCACCACTTGCATTTTCCTTGGCCCTGGTCATCCCTACCCCCCCAGgc...... : 1055462 154 : arget Intron 12 >>>> GlyLysGlyIleLeuGlyAsnAlaIleLysTrpA : 164 26791 bp |||:::||| ||| ... |||. ++GlyGlnGlySerLeuValGlyTyrThrProTrpG 1055463 : ....................agggacaggggagtctggtcggctacactccatggg : 1082284 165 : snPheThrLysPheLeuIle{A} >>>> Target Intron 13 >>>> {sp : 171 .. :::... { } 20199 bp {! lyHisIleGlnLeuAspThr{L}++ ++{ys 1082285 : gtcacatacagctggacaca{a}gt..........................ag{AG : 1102505 172 : }LysAsnGlyCysValValLysArgTyrGlyProMetGluGluProLeu{V} >> : 188 } ::!..!||| ! !::: !! !|||! !:!!! :!! !!|||{|} }ProSerSerCysProProArgAlaCysGlyArgLeuGlyGlnAlaLeu{V}-+ 1102506 : }CCTTCTTCCTGTCCACCCCGTGCATGTGGACGCCTGGGCCAAGCGCTG{G}ct.. : 1102558 189 : >> Target Intron 14 >>>> {al}IleGluLysAspLeuProHisTyrPh : 197 19461 bp {||}:!: !! !||||||! !|| ++{al}LeuThrProProArgProHisCysPh 1102559 : ........................ag{TG}CTGACCCCTCCCCGTCCTCACTGTTT : 1122043 198 : e : 197 | e 1122044 : C : 1122045 vulgar: SPP00000007_1.0 7 197 . NIVO01030317.1:subseq(555646,1182752) 570784 1122045 + 100 M 23 69 S 0 2 5 0 2 I 0 33774 3 0 2 S 1 1 M 2 6 5 0 2 I 0 62013 3 0 2 5 0 2 I 0 39080 3 0 2 M 3 9 5 0 2 I 0 22246 3 0 2 M 11 33 5 0 2 I 0 29143 3 0 2 M 13 39 5 0 2 I 0 93926 3 0 2 M 16 48 S 0 2 5 0 2 I 0 131103 3 0 2 S 1 1 M 12 36 5 0 2 I 0 5339 3 0 2 M 15 45 S 0 2 5 0 2 I 0 47621 3 0 2 S 1 1 M 20 60 S 0 2 5 0 2 I 0 6688 3 0 2 S 1 1 M 6 18 S 0 1 5 0 2 I 0 13263 3 0 2 S 1 2 M 19 57 5 0 2 I 0 26787 3 0 2 M 18 54 S 0 1 5 0 2 I 0 20195 3 0 2 S 1 2 M 16 48 S 0 1 5 0 2 I 0 19457 3 0 2 S 1 2 M 9 27 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 570785 1122045 100 + . gene_id 38 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 570785 570855 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 570785 570855 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 570856 570857 . + . intron_id 1 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 570856 604633 . + . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 604632 604633 . + . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 604634 604640 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 604634 604640 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 604641 604642 . + . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 604641 666657 . + . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 666656 666657 . + . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 666658 666659 . + . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 666658 705741 . + . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 705740 705741 . + . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 705742 705750 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 705742 705750 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 705751 705752 . + . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 705751 728000 . + . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 727999 728000 . + . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 728001 728033 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 728001 728033 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 728034 728035 . + . intron_id 5 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 728034 757180 . + . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 757179 757180 . + . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 757181 757219 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 757181 757219 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 757220 757221 . + . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 757220 851149 . + . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 851148 851149 . + . intron_id 5 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 851150 851199 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 851150 851199 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 851200 851201 . + . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 851200 982306 . + . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 982305 982306 . + . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 982307 982343 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 982307 982343 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 982344 982345 . + . intron_id 8 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 982344 987686 . + . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 987685 987686 . + . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 987687 987733 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 987687 987733 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 987734 987735 . + . intron_id 9 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 987734 1035358 . + . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1035357 1035358 . + . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1035359 1035421 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1035359 1035421 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1035422 1035423 . + . intron_id 10 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1035422 1042113 . + . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1042112 1042113 . + . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1042114 1042133 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1042114 1042133 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1042134 1042135 . + . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1042134 1055400 . + . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1055399 1055400 . + . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1055401 1055459 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1055401 1055459 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1055460 1055461 . + . intron_id 12 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1055460 1082250 . + . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1082249 1082250 . + . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1082251 1082305 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1082251 1082305 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1082306 1082307 . + . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1082306 1102504 . + . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1102503 1102504 . + . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1102505 1102555 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1102505 1102555 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1102556 1102557 . + . intron_id 14 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1102556 1122016 . + . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1122015 1122016 . + . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1122017 1122045 . + . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1122017 1122045 . + . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 570785 1122045 100 + . alignment_id 38 ; Query SPP00000007_1.0 ; Align 570785 8 69 ; Align 604635 32 6 ; Align 705742 34 9 ; Align 728001 37 33 ; Align 757181 48 39 ; Align 851150 61 48 ; Align 982308 78 36 ; Align 987687 90 45 ; Align 1035360 106 60 ; Align 1042115 127 18 ; Align 1055403 134 57 ; Align 1082251 153 54 ; Align 1102507 172 48 ; Align 1122019 189 27 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 154 Query range: 2 -> 197 Target range: 999801 -> 61996 3 : LeuGlyArgLeuCysArgLeuLeuLysPro{A} >>>> Target Intron 1 >> : 13 :::...||||||||| |||:::|||{!} 150359 bp ValAlaArgLeuCysProThrLeuArgPro{G}++ 999801 : gttgctcggttgtgcccgactctgcgaccc{g}gt....................... : 999768 14 : >> {la}LeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAla{G} >>>> Targ : 26 {.!}::: |||:::||| :::|||...||||||{|} 1 ++{ly}IleGluProGlySerLeuArgSerProAlaLeuAla{G}+- 999767 : ..ag{gg}atcgaacctgggtctcttaggtctcctgccttggca{g}gc......... : 849370 27 : et Intron 2 >>>> {ly}ThrMetCysAlaSer{Ar} >>>> Target Int : 32 26016 bp {||}|||:!!|||!.!|||{||} 170942 ++{ly}ThrValCysValSer{Ar}++ 849369 : ................ag{GT}ACTGTGTGTGTGAGC{AG}gt............... : 723335 33 : ron 3 >>>> {g}AspAspTrpArgCysAlaArgSerMet{Hi} >>>> Targe : 42 bp {|}||| ||| :::||| {..} 2 ++{g}AspGlyThrArgValSerArgValGly{Ar}++ 723334 : ..........ag{g}gatggaacccgcgtctcccgcgttggc{ag}gt.......... : 552363 43 : t Intron 4 >>>> {s}GluPheSerAlaLysAspIle >>>> Target Int : 50 8306 bp {.}||||||..!||| ! :!!! ! 166 b ++{g}GluPheGlnAlaAspAsnThr++ 552362 : ...............ag{g}GAATTTCAAGCCGACAATACAgt............... : 524035 51 : ron 5 >>>> >>>> Target Intron 6 >>>> AspGlyHisMet >>> : 54 p 46748 bp !|||.!.... ++++ ++ThrGlyGluGln++ 524034 : ..........aggt.........................agACTGGTGAACAAgt... : 477109 55 : > Target Intron 7 >>>> ValAsnLeuAspLysTyrArgGlyPheValCysI : 65 173097 bp ||||||||| ||| !||| ||| ||| ++ValAsnLeuArgLysValArgPhePhePheCysS 477108 : ......................agGTGAACTTGCGGAAGGTCcgtttcttcttttgtt : 303981 66 : leValThrAsnValAlaSerGlnUnkGly{L} >>>> Target Intron 8 >>> : 75 ..!:!! ! |||...:::...|||{ } 24064 bp erThrSerAlaCysAlaGlyArgAlaGly{G}++ 303980 : ctacTTCCGCCTgcgcgggccgggccggg{g}gt........................ : 303948 76 : > {ys}ThrGluValAsnTyrThrGln >>>> Target Intron 9 >>>> L : 83 { !} ! !|||||| ! ! !!:! 39681 bp ++{ly}ArgThrValAsnGlnLysArg++ ++T 303947 : .ag{gg}cgAACAGTGAATCAAAAACGGgt.........................agA : 240182 84 : euValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArg{Il} >>>> Target : 97 !:!! !!!!||| !||| ! .!!! !||| !||| !{:!} 1 hrLeuThrPheHisArgArgGluThrAlaCysHisLeuLeu{Le}++ 240181 : CACTGACCTTCCACCGAAGAGAGACAGCATGTCATTTGTTA{TT}gt........... : 240136 98 : Intron 10 >>>> {e}LeuAlaPheProCysAsnGlnPheGlyLysGlnGluPr : 110 540 bp {:}! !|||!!.|||! !!..||| !:::|||||| ++{u}SerAlaLeuProPheArgGlnGlyProGlnGlnGluGl 240135 : ...............ag{G}TCAGCATTGCCCTTCAGGCAGGGGCCAcagcaggagga : 238561 111 : oGlySerAsnGlu{G} >>>> Target Intron 11 >>>> {lu}IleLys : 118 |||... !..!{!} 4820 bp {!:}:!::!! uGlyLysAlaArg{A}++ ++{sp}LeuGln++ 238560 : ggggaaagCCCGG{G}gt..........................ag{AT}CTGCAAgt : 233717 119 : >>>> Target Intron 12 >>>> GluPheAlaAlaGlyTyrAsnValLysPhe : 127 20420 bp ||| !|||::! ! ! ! |||||| ++GluHisAlaSerHisLeu***ValLysAla 233716 : ..........................agGAGCATGCAAGCCACCTCTAAGTGAAAGCA : 213270 128 : {A} >>>> Target Intron 13 >>>> {sp}MetPheSerLysIleCysVal : 135 {|} 62497 bp {||} |||::!:!!|||||| {A}++ ++{sp}ProGlySerArgValCysVal 213269 : {G}gt..........................ag{AT}CCAGGGTCCCGAGTTTGTGTG : 150749 136 : AsnGlyAsp{A} >>>> Target Intron 14 >>>> {sp}AlaHisProLeu : 143 .!.|||! {!} 23581 bp {!:} !||| !:!! GluGlyGly{G}++ ++{lu}LeuHisSerVal 150748 : GAGGGTGGA{G}gt..........................ag{AA}CTACATAGCGTG : 127144 144 : TrpLysTrpMetLysIleGlnProLys{G} >>>> Target Intron 15 >>>> : 153 !!:!|||! !:!:!: !|||!:!{|} 21275 bp GlnArgTrpArgArgValGlyProArg{G}+- 127143 : CAGAGATGGAGAAGGGTGGGGCCAAGA{G}ga.......................... : 127111 154 : {ly}LysGlyIleLeu{G} >>>> Target Intron 16 >>>> {ly}Asn : 159 {||} :::|||{|} 2783 bp {||}!.! ++{ly}CysLeuValLeu{G}++ ++{ly}Thr 127110 : ag{ga}tgtctggttcta{g}gt..........................ag{GG}ACC : 103038 160 : AlaIleLysTrpAsnPheThr >>>> Target Intron 17 >>>> LysPheL : 169 :!! !:!!|||||| ||| 13385 bp !:!!::: SerArgGluTrpAsnGlnThr++ ++ArgTrpV 103037 : TCCCGTGAGTGGAATCAGACAgt..........................agAGGTGGG : 89623 170 : euIleAspLysAsnGlyCysValValLysArgTyr >>>> Target Intron 18 : 181 !!!!:! ! .!.||||||:!!:!! !! !!:: 27540 bp alMetAlaProGlnGlyCysLeuLeuGlyProTrp++ 89622 : TGATGGCTCCTCAGGGCTGTCTACTTGGGCCCTGGgt..................... : 89585 182 : >>>> GlyProMetGluGluProLeuValIleGluLysAspLeuProHisTyrPhe : 197 ||||||! |||!!:|||! !!.!:!!.......!. !|||! !! ||| ++GlyProThrGluAspProGlnAlaLeuSerSerGlnAspProProLeuPhe 89584 : .....agGGGCCCACAGAGGATCCTCAAGCTCTATCCTCCCAGGATCCACCCTTGTTC : 61999 vulgar: SPP00000007_1.0 2 197 . NIVO01030317.1:subseq(555646,1182752) 999801 61996 - 154 M 10 30 S 0 1 5 0 2 I 0 150355 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 126012 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 170938 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 28302 3 0 2 S 1 1 M 7 21 5 0 2 I 0 162 3 0 2 5 0 2 I 0 46744 3 0 2 M 4 12 5 0 2 I 0 173093 3 0 2 M 21 63 S 0 1 5 0 2 I 0 24060 3 0 2 S 1 2 M 7 21 5 0 2 I 0 39677 3 0 2 M 14 42 S 0 2 5 0 2 I 0 1536 3 0 2 S 1 1 M 17 51 S 0 1 5 0 2 I 0 4816 3 0 2 S 1 2 M 2 6 5 0 2 I 0 20416 3 0 2 M 10 30 S 0 1 5 0 2 I 0 62493 3 0 2 S 1 2 M 10 30 S 0 1 5 0 2 I 0 23577 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 21271 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 2779 3 0 2 S 1 2 M 8 24 5 0 2 I 0 13381 3 0 2 M 14 42 5 0 2 I 0 27536 3 0 2 M 17 51 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 61997 999801 154 - . gene_id 1 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 999771 999801 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 999771 999801 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 999769 999770 . - . intron_id 1 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 849412 999770 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 849412 849413 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 849373 849411 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 849373 849411 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 849371 849372 . - . intron_id 2 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 723357 849372 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 723357 723358 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 723338 723356 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 723338 723356 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 723336 723337 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 552396 723337 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 552396 552397 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 552366 552395 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 552366 552395 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 552364 552365 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 524060 552365 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 524060 524061 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 524038 524059 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 524038 524059 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 524036 524037 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 524037 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 523871 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 304015 477111 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 304015 304016 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 303951 304014 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 303951 304014 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 303949 303950 . - . intron_id 8 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 279887 303950 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 279887 279888 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 279864 279886 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 279864 279886 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 279862 279863 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 240183 279863 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 240183 240184 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 240139 240182 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 240139 240182 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 240137 240138 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 238599 240138 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 238599 238600 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 238546 238598 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 238546 238598 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 238544 238545 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 233726 238545 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 233726 233727 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 233718 233725 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 233718 233725 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 233716 233717 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 213298 233717 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 213298 213299 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 213267 213297 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 213267 213297 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 213265 213266 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 150770 213266 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 150770 150771 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 150737 150769 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 150737 150769 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 150735 150736 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 127156 150736 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 127156 127157 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 127114 127155 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 127114 127155 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 127112 127113 . - . intron_id 15 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 105839 127113 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 105839 105840 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 105824 105838 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 105824 105838 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 105822 105823 . - . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 103041 105823 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 103041 103042 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 103015 103040 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 103015 103040 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 103013 103014 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 89630 103014 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 89630 89631 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 89588 89629 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 89588 89629 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 89586 89587 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 62048 89587 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 62048 62049 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 61997 62047 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 61997 62047 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 61997 999801 154 - . alignment_id 1 ; Query SPP00000007_1.0 ; Align 999802 3 30 ; Align 849410 14 36 ; Align 723355 27 15 ; Align 552395 33 27 ; Align 524059 43 21 ; Align 477124 50 12 ; Align 304015 54 63 ; Align 279885 76 21 ; Align 240183 83 42 ; Align 238598 98 51 ; Align 233724 116 6 ; Align 213298 118 30 ; Align 150768 129 30 ; Align 127154 140 39 ; Align 105837 154 12 ; Align 103039 159 24 ; Align 89630 167 42 ; Align 62048 181 51 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 156 Query range: 1 -> 195 Target range: 1158378 -> 956 2 : SerLeuGlyArgLeu{C} >>>> Target Intron 1 >>>> {ys}ArgLe : 9 |||! !||||||! !{ } 126252 bp {! }:::|| SerCysGlyArgPro{G}++ ++{ly}LysLe 1158378 : AGCTGCGGCCGCCCA{G}gt.........................ag{gg}aaact : 1032105 10 : uLeuLysProAlaLeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAlaGlyThrM : 28 | ...|||...::: |||::: ...:::|||...||||||||| uProAsnProGlyMetGluProGlySerProGlySerProAlaLeuAlaGlyGlyS 1032104 : tcccaacccagggatggaacctgggtctcctgggtctcctgcgttggcaggtgggt : 1032048 29 : etCysAlaSerArgAspAsp{Tr} >>>> Target Intron 2 >>>> {p} : 35 ...::: {||} 15436 bp {|} erPheThrThrSerAlaAla{Tr}+- ++{p} 1032047 : cctttaccactagcgccgcc{tg}ga.........................ag{G} : 1016589 36 : ArgCys{Al} >>>> Target Intron 3 >>>> {a}ArgSerMetHisGl : 43 ||||||{ } 59812 bp {!}||||||:::|||.. ArgCys{Ar}++ ++{g}ArgSerLeuHisHi 1016588 : CGCTGT{AG}gt.........................ag{g}cgaagtctacacca : 956755 44 : uPheSerAla{Ly} >>>> Target Intron 4 >>>> {s}AspIleAspG : 51 .::::::|||{!:} 107000 bp {!}|||::: sTrpAlaAla{Ar}+- ++{g}AspValLeuT 956754 : ctgggctgcc{ag}gg.........................ag{g}gacgttcttt : 849731 52 : lyHisMetValAsnLeu >>>> Target Intron 5 >>>> AspLysTyrA : 60 |||::: ::: 5841 bp ! !..! | yrHisLeuSerHisGln+- ++LysSerLeuA 849730 : accatctgagccaccaggg.........................agAAAAGCTTGA : 843863 61 : rg{G} >>>> Target Intron 6 >>>> {ly}PheValCysIleValThr : 67 ||{|} 51007 bp {||}|||:!!|||:!! ! ! rg{G}++ ++{ly}PheLeuCysLeu***Gly 843862 : GA{G}gt.........................ag{GC}TTCCTGTGTTTATGAGGC : 792835 68 : AsnValAlaSerGlnUnkGlyLysThrGluValAsn{T} >>>> Target Int : 80 :!!..!! !..!:!! !|||! ! ! |||{ } 3528 b HisThrGluAspGluAsnGlnLysMetProGlnAsn{A}++ 792834 : CACACAGAAGATGAGAATCAGAAAATGCCACaaaat{g}gt............... : 792793 81 : ron 7 >>>> {yr}ThrGlnLeuValAspLeuHisAla{A} >>>> Target : 89 p {!!}||| ! !!.! !! !|||:!!{ } 61 ++{sp}ThrCysTrpAlaProTyrHisSer{A}++ 792792 : ..........ag{AC}ACTTGCTGGGCGCCCTACCATTCA{G}gt........... : 789238 90 : Intron 8 >>>> {rg}TyrAlaGluCysGlyLeuArgIleLeuAlaPhePro : 101 889 bp { !} !!:!!:!! ! !|||:!: !! !.!!||| ++{sp}AspSerLysMetLeuAlaArgValArgGluLeuPro 789237 : ..............ag{AC}GATTCCAAAATGCTGGCTAGAGTGAGAGAGCTTCCC : 727316 102 : CysAsnGlnPhe{G} >>>> Target Intron 9 >>>> {ly}LysGlnGl : 109 ||| !:!!! {|} 54867 bp {||} :::|| CysAlaLysSer{G}++ ++{ly}ThrGluGl 727315 : TGTGCTAAATCG{G}gt.........................ag{gg}acagagga : 672425 110 : uPro{G} >>>> Target Intron 10 >>>> {ly}SerAsnGluGluIle : 116 ||||{|} 88117 bp {||}! !.!.|||||| ! uPro{G}++ ++{ly}MetGluGluGluGly 672424 : gcct{g}gt..........................ag{GA}ATGGAAGAGGAAGGT : 584287 117 : LysGluPheAla{Al} >>>> Target Intron 11 >>>> {a}GlyTyrA : 124 ! !|||.!! !{||} 13648 bp {|}||| : ThrGluIleGln{Al}++ ++{a}GlySerS 584286 : ACGGAAATCCAA{GC}gt..........................ag{g}ggatctt : 570615 125 : snValLysPheAspMetPheSerLysIleCysValAsn{G} >>>> Target I : 137 :: |||... |||::: {|} 1435 erProProArgAspGlnThrArgValSerCysIleAla{G}++ 570614 : ccccacccagggatcaaacccgggtctcctgcatcgca{g}gt............. : 570573 138 : ntron 12 >>>> {ly}AspAspAlaHisPro >>>> Target Intron 1 : 143 97 bp {||}||| |||||| 53874 bp ++{ly}AspProProHisPro++ 570572 : .............ag{gg}gatcctccccacccagt.................... : 426959 144 : 3 >>>> LeuTrpLysTrpMet{L} >>>> Target Intron 14 >>>> : 148 ||||||!..||| { } 35686 bp ++LeuTrpSerTrpPro{G}++ ++ 426958 : ......agCTTTGGAGCTggcct{g}gt..........................ag : 337387 149 : {ys}IleGlnPro{Ly} >>>> Target Intron 15 >>>> {s}GlyLys : 154 { !}|||:::|||{!:} 39393 bp {!}|||::! {ly}IleGluPro{Ar}++ ++{g}GlyArg 337386 : {gg}attgaaccc{ag}gt..........................ag{G}GGGCGG : 297975 155 : GlyIleLeuGlyAsnAlaIleLysTrp{As} >>>> Target Intron 16 > : 164 ..!||| ! ! !! ! !!::{!.} 31471 bp SerIleAlaThrTrpAsnThrAlaTyr{Ar}++ 297974 : TCCATAGCCACCTGGAACACAGCATAC{AG}gt....................... : 297941 165 : >>> {n}PheThrLysPheLeuIle >>>> Target Intron 17 >>>> : 171 {.}.!!!:!||| |||:!: 56494 bp ++{g}LeuSerLysArgLeuLeu++ ++ 297940 : ...ag{A}CTTAGCAAGAGGCTGCTGgt..........................ag : 209961 172 : AspLysAsnGlyCysValValLysArgTyrGlyPro{Me} >>>> Target In : 183 |||! !:!!||| :!!..! !!:!!::||||||{! } 53792 AspThrAspGlyLeuIleThrProGlnTrpGlyPro{Ar}++ 209960 : GACACAGATGGGCTAATCACACCACAGTGGGGACCC{AG}gt.............. : 209919 184 : tron 18 >>>> {t}Glu{Gl} >>>> Target Intron 19 >>>> {u : 185 bp {!}:::{..} 135506 bp {! ++{g}Lys{Ar}++ ++{g 209918 : ............ag{g}aaa{ag}gt..........................ag{G : 20617 186 : }ProLeuValIle{Gl} >>>> Target Intron 20 >>>> {u}LysAsp : 192 }||| ! !:!:{..} 19630 bp {!}:!!.!. }ProThrCysVal{Ar}++ ++{g}GlnGln 20616 : }CCCACCTGTGTG{AG}gt..........................ag{G}CAACAG : 968 193 : LeuProHis : 195 ! !|||||| GlnProHis 967 : CAGCCCCAC : 957 vulgar: SPP00000007_1.0 1 195 . NIVO01030317.1:subseq(555646,1182752) 1158378 956 - 156 M 5 15 S 0 1 5 0 2 I 0 126248 3 0 2 S 1 2 M 27 81 S 0 2 5 0 2 I 0 15432 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 59808 3 0 2 S 1 1 M 8 24 S 0 2 5 0 2 I 0 106996 3 0 2 S 1 1 M 9 27 5 0 2 I 0 5837 3 0 2 M 4 12 S 0 1 5 0 2 I 0 51003 3 0 2 S 1 2 M 18 54 S 0 1 5 0 2 I 0 3524 3 0 2 S 1 2 M 8 24 S 0 1 5 0 2 I 0 61885 3 0 2 S 1 2 M 16 48 S 0 1 5 0 2 I 0 54863 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 88113 3 0 2 S 1 2 M 9 27 S 0 2 5 0 2 I 0 13644 3 0 2 S 1 1 M 15 45 S 0 1 5 0 2 I 0 143593 3 0 2 S 1 2 M 5 15 5 0 2 I 0 53870 3 0 2 M 5 15 S 0 1 5 0 2 I 0 35682 3 0 2 S 1 2 M 3 9 S 0 2 5 0 2 I 0 39389 3 0 2 S 1 1 M 11 33 S 0 2 5 0 2 I 0 31467 3 0 2 S 1 1 M 6 18 5 0 2 I 0 56490 3 0 2 M 12 36 S 0 2 5 0 2 I 0 53788 3 0 2 S 1 1 M 1 3 S 0 2 5 0 2 I 0 135502 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 19626 3 0 2 S 1 1 M 5 15 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 957 1158378 156 - . gene_id 2 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1158363 1158378 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1158363 1158378 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1158361 1158362 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1032111 1158362 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1032111 1032112 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1032026 1032110 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1032026 1032110 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1032024 1032025 . - . intron_id 2 ; splice_site "ga" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016590 1032025 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016590 1016591 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016589 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016589 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 956769 1016580 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 956769 956770 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 956742 956768 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 956742 956768 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 956740 956741 . - . intron_id 4 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 849742 956741 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 849742 849743 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 849714 849741 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 849714 849741 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 849712 849713 . - . intron_id 5 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 843873 849713 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 843873 843874 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 843860 843872 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 843860 843872 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 843858 843859 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 792853 843859 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 792853 792854 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 792796 792852 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 792796 792852 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 792794 792795 . - . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 789268 792795 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 789268 789269 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 789241 789267 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 789241 789267 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 789239 789240 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 727352 789240 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 727352 727353 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 727301 727351 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 727301 727351 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 727299 727300 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 672434 727300 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 672434 672435 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 672419 672433 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 672419 672433 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 672417 672418 . - . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 584302 672418 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 584302 584303 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 584271 584301 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 584271 584301 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 584269 584270 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 570623 584270 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 570623 570624 . - . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 570576 570622 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 570576 570622 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 570574 570575 . - . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 426979 570575 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 426979 426980 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 426962 426978 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 426962 426978 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 426960 426961 . - . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 373088 426961 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 373088 373089 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 373072 373087 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 373072 373087 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 373070 373071 . - . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 337386 373071 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 337386 337387 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 337373 337385 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 337373 337385 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 337371 337372 . - . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297980 337372 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297980 297981 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297944 297979 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297944 297979 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297942 297943 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 266473 297943 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 266473 266474 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 266454 266472 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 266454 266472 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 266452 266453 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 209960 266453 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 209960 209961 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 209922 209959 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 209922 209959 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 209920 209921 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 156130 209921 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 156130 156131 . - . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 156124 156129 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 156124 156129 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 156122 156123 . - . intron_id 19 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 20618 156123 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 20618 20619 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 20603 20617 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 20603 20617 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 20601 20602 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 973 20602 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 973 974 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 957 972 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 957 972 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 957 1158378 156 - . alignment_id 2 ; Query SPP00000007_1.0 ; Align 1158379 2 15 ; Align 1032109 8 81 ; Align 1016589 36 6 ; Align 956768 39 24 ; Align 849741 48 27 ; Align 843873 57 12 ; Align 792851 62 54 ; Align 789266 81 24 ; Align 727350 90 48 ; Align 672432 107 12 ; Align 584300 112 27 ; Align 570622 122 45 ; Align 426977 138 15 ; Align 373088 143 15 ; Align 337384 149 9 ; Align 297979 153 33 ; Align 266472 165 18 ; Align 209960 171 36 ; Align 156129 184 3 ; Align 20617 186 12 ; Align 972 191 15 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 151 Query range: 0 -> 187 Target range: 1039280 -> 4724 1 : MetSerLeu{Gl} >>>> Target Intron 1 >>>> {y}ArgLeuCys{A : 8 :!:!!!|||{.!} 22677 bp {!}|||! !|||{| LeuCysLeu{Se}++ ++{r}ArgArgCys{A 1039280 : CTTTGTCTA{AG}gt.........................ag{T}AGGCGCTGT{A : 1016582 9 : r} >>>> Target Intron 2 >>>> {g}LeuLeuLysProAlaLeu{Le} : 15 |} 92785 bp {|}||||||...|||! ! !{||} r}++ ++{g}LeuLeuSerProGluArg{Le} 1016581 : G}gt.........................ag{A}CTACTTTCCCCCGAAAGA{CT} : 923776 16 : >>>> Target Intron 3 >>>> {u}CysGlyAlaLeuAlaAlaProGly : 23 32371 bp {|}|||||||||!!! ||| ++ ++{u}CysGlyAlaPheLeuAlaAlaGln 923775 : gt.........................ag{g}tgtGGGGCTTTCCtagcagctcag : 891381 24 : Leu >>>> Target Intron 4 >>>> AlaGlyThrMetCysAla{S} > : 31 ||| 113528 bp |||!.! !:!!||||||{.} Leu++ ++AlaAlaLeuLeuCysAla{G}+- 891380 : ttggt.........................agGCAGCCCTCCTGTGTGCA{G}gg. : 777826 32 : >>> Target Intron 5 >>>> {er}Arg{A} >>>> Target Intron : 33 8122 bp {!!}|||{!} 24592 bp ++{ly}Arg{G}++ 777825 : ........................ag{gg}agg{g}gt.................. : 769698 34 : 6 >>>> {sp}AspTrpArgCysAlaArgSerMetHisGluPheSerAlaLysA : 48 { }||| ||| ... :::... . ++{ly}AspLeuProAsnProGlySerGluProArgSerAlaThrLeuG 769697 : .......ag{gg}gatcttcccaacccagggagcgaacccagatctgccacattgc : 745064 49 : spIleAspGlyHisMetValAsnLeuAspLysTyrArgGlyPheValCysIleVal : 66 .. |||... :::...... ::: !|||! ||||||! !||| lnAlaAspSerLeuLeuThrGluAlaProArgLysLeuGlySerValCysSerVal 745063 : aggcggattctttactaactgaggcaccaaggaagctGGGTTCAGTATGCAGTGTG : 745010 67 : ThrAsnValAlaSerGln >>>> Target Intron 7 >>>> UnkGly{L} : 75 !.!!:! ! ! !||| 16852 bp ..!{ } AsnSerSerLeuLeuGln++ ++AspSer{G} 745009 : AATAGCAGTCTTCTACAGgt.........................agGATTCA{G} : 728131 76 : >>>> Target Intron 8 >>>> {ys}ThrGluValAsnTyrThrGlnLe : 83 127550 bp { !}! !||||||!.!!:! ! ! || ++ ++{ly}LysGluValThrPheLeuTyrLe 728130 : gt.........................ag{GA}AAGGAAGTAACTTTTCTCTACCT : 600557 84 : uVal >>>> Target Intron 9 >>>> AspLeuHis >>>> Target : 88 | ! 54503 bp !!:|||!!. 221 uGln+- ++GluLeuGln++ 600556 : TCAGga.........................agGAGCTGCAGgt............ : 546037 89 : Intron 10 >>>> >>>> Target Intron 11 >>>> AlaArgTyrA : 91 68 bp 14571 bp ! ! !!! !! ++++ ++GluGlySerG 546036 : ..............aggt..........................agGAGGGGTCTG : 509291 92 : laGluCysGlyLeuArgIleLeuAlaPhePro >>>> Target Intron 12 : 102 !||| !|||:!!|||! !|||!.! ||| 13716 bp luGluThrGlyIleArgSerLeuGlyGluPro++ 509290 : AAGAGACTGGGATCAGAAGCCTTGGGGAGCCGgt...................... : 509256 103 : >>>> Cys >>>> Target Intron 13 >>>> AsnGlnPheGlyLysGl : 108 6861 bp ..!...|||..!|||:: ++Leu++ ++GlySerPheSerLysAr 509255 : ....agCTGgt..........................agGGCAGCTTTTCCAAGAG : 488663 109 : nGluPro >>>> Target Intron 14 >>>> GlySerAsnGluGluIleL : 117 !|||||| 143036 bp ||||||||| gGluPro++ ++GlySerAsnProValCysL 488662 : AGAACCCgt..........................agggatcgaaccctgtgtgtc : 345600 118 : ysGluPheAlaAlaGlyTyrAsnVal{L} >>>> Target Intron 15 >>> : 126 ...|||||||||:::::::::{ } 102620 bp euAlaLeuAlaAlaGlyPheSerIle{G}-+ 345599 : ttgcattggcagctggattctctatc{g}tt......................... : 345570 127 : > {ys}PheAspMetPhe{Se} >>>> Target Intron 16 >>>> {r} : 131 { } !! !! |||{||} 31118 bp {|} ++{ly}ValHisSerPhe{Se}+- ++{r} 345569 : .ag{GT}GTTCACTCCTTC{AG}gg..........................ag{t} : 211818 132 : LysIleCysValAsnGlyAspAspAlaHis >>>> Target Intron 17 >> : 142 ||| !..! !:!!||| !!! ||| 26590 bp LysGlyAlaCysHisGlyHisGlyAlaSer++ 211817 : AAAGGAGCCTGTCATGGTCACGGGGCATCGgt........................ : 211785 143 : >> ProLeuTrpLysTrp >>>> Target Intron 18 >>>> MetLysI : 149 ! !! !|||!:!||| 29053 bp |||: ++ArgArgTrpArgTrp++ ++GluLysV 211784 : ..agCGGCGGTGGAGGTGGgt..........................aggaaaagg : 156123 150 : leGlnProLysGly{L} >>>> Target Intron 19 >>>> {ys}GlyIl : 156 ::... ||||||{|} 17075 bp {||}||| alHisGlyLysGly{L}-+ -+{ys}GlyAl 156122 : tacatggaaaagga{a}at..........................gg{AG}GGCGC : 139027 157 : eLeuGlyAsnAlaIle{Ly} >>>> Target Intron 20 >>>> {s}Trp : 163 ||||||!..|||:!!{!:} 42511 bp {!}||| aLeuGlyArgAlaVal{Ar}++ ++{g}Trp 139026 : GTTGGGGAGGGCGGTC{AG}gt..........................ag{g}tgg : 96495 164 : Asn{Ph} >>>> Target Intron 21 >>>> {e}ThrLysPheLeuIleA : 171 ...{ } 32147 bp {!}||| !.!!||||||. Arg{As}-+ ++{n}ThrProLeuLeuIleS 96494 : aga{aa}ct..........................ag{C}ACCCCACTTCTCATCA : 64324 172 : spLysAsn >>>> Target Intron 22 >>>> >>>> Target Intr : 174 .! ! .!. 22367 bp 24342 b erTyrGln++ ++++ 64323 : GCTACCAGgt..........................aggt................ : 41946 175 : on 23 >>>> {G} >>>> Target Intron 24 >>>> {ly}CysValV : 177 p {|} 2746 bp {||}||| ++{G}++ ++{ly}CysGluA 41945 : ..........ag{G}gt..........................ag{gc}tgtgaga : 14851 178 : alLysArgTyrGlyProMet{G} >>>> Target Intron 25 >>>> {lu : 184 |||::: {|} 10094 bp {|| rgAspArgHisArgGlnArg{G}++ ++{lu 14850 : gagacagacacagacagaga{g}gt..........................ag{AG : 4735 185 : }GluProLeu : 187 }|||! !||| }GluGlnLeu 4734 : }GAGCAGCTG : 4725 vulgar: SPP00000007_1.0 0 187 . NIVO01030317.1:subseq(555646,1182752) 1039280 4724 - 151 M 3 9 S 0 2 5 0 2 I 0 22673 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 92781 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 32367 3 0 2 S 1 1 M 9 27 5 0 2 I 0 113524 3 0 2 M 6 18 S 0 1 5 0 2 I 0 8118 3 0 2 S 1 2 M 1 3 S 0 1 5 0 2 I 0 24588 3 0 2 S 1 2 M 39 117 5 0 2 I 0 16848 3 0 2 M 2 6 S 0 1 5 0 2 I 0 127546 3 0 2 S 1 2 M 9 27 5 0 2 I 0 54499 3 0 2 M 3 9 5 0 2 I 0 22164 3 0 2 5 0 2 I 0 14567 3 0 2 M 14 42 5 0 2 I 0 13712 3 0 2 M 1 3 5 0 2 I 0 6857 3 0 2 M 8 24 5 0 2 I 0 143032 3 0 2 M 15 45 S 0 1 5 0 2 I 0 102616 3 0 2 S 1 2 M 4 12 S 0 2 5 0 2 I 0 31114 3 0 2 S 1 1 M 10 30 5 0 2 I 0 26586 3 0 2 M 5 15 5 0 2 I 0 29049 3 0 2 M 7 21 S 0 1 5 0 2 I 0 17071 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 42507 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 32143 3 0 2 S 1 1 M 8 24 5 0 2 I 0 22363 3 0 2 5 0 2 I 0 24338 3 0 2 S 0 1 5 0 2 I 0 2742 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 10090 3 0 2 S 1 2 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 4725 1039280 151 - . gene_id 3 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1039270 1039280 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1039270 1039280 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1039268 1039269 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016593 1039269 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016593 1016594 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016592 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016592 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 923796 1016580 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 923796 923797 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 923775 923795 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 923775 923795 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 923773 923774 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 891404 923774 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 891404 891405 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 891376 891403 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 891376 891403 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 891374 891375 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 777848 891375 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 777848 777849 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 777829 777847 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 777829 777847 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 777827 777828 . - . intron_id 5 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 769707 777828 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 769707 769708 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 769701 769706 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 769701 769706 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 769699 769700 . - . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 745109 769700 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 745109 745110 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 744990 745108 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 744990 745108 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 744988 744989 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 728138 744989 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 728138 728139 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 728131 728137 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 728131 728137 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 728129 728130 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 600581 728130 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 600581 600582 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 600552 600580 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 600552 600580 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 600550 600551 . - . intron_id 9 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 600551 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 546039 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 509301 523871 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 509301 509302 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 509259 509300 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 509259 509300 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 509257 509258 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 495543 509258 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 495543 495544 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 495540 495542 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 495540 495542 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 495538 495539 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 488679 495539 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 488679 488680 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 488655 488678 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 488655 488678 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 488653 488654 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 345619 488654 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 345619 345620 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 345573 345618 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 345573 345618 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 345571 345572 . - . intron_id 15 ; splice_site "tt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 242953 345572 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 242953 242954 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 242937 242952 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 242937 242952 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 242935 242936 . - . intron_id 16 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 211819 242936 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 211819 211820 . - . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 211788 211818 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 211788 211818 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 211786 211787 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 185198 211787 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 185198 185199 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 185183 185197 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 185183 185197 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 185181 185182 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 156130 185182 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 156130 156131 . - . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 156108 156129 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 156108 156129 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 156106 156107 . - . intron_id 19 ; splice_site "at" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 139033 156107 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 139033 139034 . - . intron_id 18 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 139008 139032 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 139008 139032 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 139006 139007 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 96497 139007 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 96497 96498 . - . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 96488 96496 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 96488 96496 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 96486 96487 . - . intron_id 21 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64341 96487 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64341 64342 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64316 64340 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64316 64340 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64314 64315 . - . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 64315 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 17607 41948 . - . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 17607 17608 . - . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 17606 17606 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 17606 17606 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 17604 17605 . - . intron_id 24 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 14860 17605 . - . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 14860 14861 . - . intron_id 23 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 14830 14859 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 14830 14859 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 14828 14829 . - . intron_id 25 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 4736 14829 . - . intron_id 25 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 4736 4737 . - . intron_id 24 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 4725 4735 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 4725 4735 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 4725 1039280 151 - . alignment_id 3 ; Query SPP00000007_1.0 ; Align 1039281 1 9 ; Align 1016592 5 9 ; Align 923795 9 18 ; Align 891403 16 27 ; Align 777848 25 18 ; Align 769705 32 3 ; Align 745107 34 117 ; Align 728138 73 6 ; Align 600579 76 27 ; Align 546049 85 9 ; Align 509301 88 42 ; Align 495543 102 3 ; Align 488679 103 24 ; Align 345619 111 45 ; Align 242951 127 12 ; Align 211818 132 30 ; Align 185198 142 15 ; Align 156130 147 21 ; Align 139031 155 21 ; Align 96496 163 6 ; Align 64340 166 24 ; Align 14858 175 27 ; Align 4734 185 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 152 Query range: 0 -> 176 Target range: 546062 -> 5734 1 : MetSerLeuGlyArgLeuCys{Ar} >>>> Target Intron 1 >>>> {g}L : 9 :!:!!!:!!..!||| !|||{||} 68916 bp {|}| LeuCysValSerArgSerCys{Ar}++ ++{g}L 546062 : CTCTGCGTCTCCAGGAGCTGC{AG}gt.........................ag{A}C : 477122 10 : eu >>>> Target Intron 2 >>>> LeuLysProAlaLeu{Le} >>>> T : 15 || 9662 bp ||| !||||||:!!{||} eu++ ++LeuProProAlaVal{Le}++ 477121 : TGgt.........................agCTGCCGCCCGCCGTC{CT}gt...... : 467438 16 : arget Intron 3 >>>> {u}Cys{G} >>>> Target Intron 4 >>>> : 17 620 bp {|}|||{|} 25308 bp ++{u}Cys{G}++ + 467437 : ...................ag{c}tgc{g}gt.........................a : 441509 18 : {ly}AlaLeuAlaAlaPro >>>> Target Intron 5 >>>> GlyLeuAla : 25 {||} |||||| ||| 8519 bp |||:::::: +{ly}AspLeuAlaHisPro+- ++GlyIleSer 441508 : g{gg}gatcttgcccacccagg.........................aggggatctct : 432965 26 : GlyThrMetCysAlaSerArgAspAspTrpArgCysAlaArgSerMetHisGlu >> : 44 ...|||... :::::: |||...||| ||| :::|||::: AlaThrGlnGlySerAsnProGlyLeuTrpHisCysArgArgLeuLeuHisGln-+ 432964 : gcaacccagggatcaaacccaggtctctggcattgcaggcggcttcttcaccagct.. : 432906 45 : >> Target Intron 6 >>>> PheSerAlaLysAspIleAsp{G} >>>> Ta : 51 36617 bp |||... |||::: {|} ++GluSerCysMetAspValArg{G}++ 432905 : .......................aggagtcgtgtatggatgtgaga{g}gt....... : 396267 52 : rget Intron 7 >>>> {ly}HisMetValAsnLeuAspLysTyrArg{G} >> : 61 149225 bp {||}:!! ! !:!! !|||||| ! |||{|} ++{ly}TyrCysAspHisThrAspLysGlnArg{G}++ 396266 : ..................ag{GT}TACTGTGACCACACTGATAAGCAAAGA{G}gt.. : 247012 62 : >> Target Intron 8 >>>> {ly}PheValCysIleValThrAsn{V} >>> : 69 34100 bp {||}.!. !|||! !:!! !!:!{!} ++{ly}IleProCysArgLeuGluSer{G}++ 247011 : .......................ag{GA}ATACCATGCAGACTGGAAAGT{G}gt... : 212888 70 : > Target Intron 9 >>>> {al}AlaSerGlnUnkGlyLysThrGluValAsn : 79 9347 bp { !}||||||! !||||||! !|||::! ++{ly}AlaSerPro***ThrLysThrGlyValSer 212887 : ......................ag{GG}GCCTCCCCCTGAACAAAGACGGGGGTCTCT : 203514 80 : TyrThrGlnLeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArgIle >> : 98 !||| !:!! ! !.! !!||| !!!:||| !|||||| LeuThrAlaAsnLeuThrGluSerGlyGlyTyrGlnAspCysTyrLeuArgGlu+- 203513 : CTTACAGCCAACCTGACGGAGTCGGGGGGTTACCAGGACTGTTACCTCCGGGAGgg.. : 203455 99 : >> Target Intron 10 >>>> LeuAlaPheProCysAsnGlnPheGlyLysGl : 108 19416 bp ||| ||||||::: ...|||!:!:! -+LeuProAlaProCysSerLeuLeuGlyArgGl 203454 : ........................ggctccctgctccctgctccctgctcGGCAGGGA : 184011 109 : nGluProGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyr{A} >>>> Ta : 124 !|||||| !|||||| !.!.:!:! ... |||!.!||| {.} uGluPro***SerAsnProHisValThrSerGlyAlaGlyGlyAla{G}+- 184010 : AGAACCGTAATCAAACCCACATGTGACCAGTGGGGCGGGTGGTGCA{G}gg....... : 183960 125 : rget Intron 11 >>>> {sn}ValLys{Ph} >>>> Target Intron 12 : 127 21217 bp {..}:!!:!!{ } 59695 bp ++{ly}IleGln{Ar}++ 183959 : ...................ag{GA}ATACAA{AG}gt..................... : 162733 128 : >>>> {e}AspMet{Ph} >>>> Target Intron 13 >>>> {e}SerLy : 132 { }|||:!:{ } 30827 bp { } !!. ++{g}AspLeu{Pr}++ ++{o}ValSe 162732 : .....ag{G}GACCTC{CC}gt..........................ag{G}GTGAG : 72200 133 : sIleCysValAsnGlyAspAspAlaHisProLeuTrpLysTrpMetLysIle{Gl} : 150 .!!:||| !.!.||| !..! !|||||| !||| ||||||!:! {! } rMetCysProGluGlyProSerArgHisProArgTrpAlaTrpMetArgAla{Le}++ 72199 : CATGTGCCCTGAGGGTCCCTCCAGGCATCCCAGGTGGGCCTGGATGAGGGCG{CT}gt : 72144 151 : >>>> Target Intron 14 >>>> {n}ProLys >>>> Target Intron : 153 12779 bp { }|||::: 17410 bp ++{u}ProGln++ 72143 : ..........................ag{t}ccacaggt................... : 59356 154 : 15 >>>> >>>> Target Intron 16 >>>> GlyLysGly{I} >>>> : 156 13148 bp |||||||||{ } ++++ ++GlyLysGly{S}++ 59355 : .......aggt..........................agGGGAAGGGG{T}gt..... : 28788 157 : Target Intron 17 >>>> {le}LeuGlyAsnAlaIleLysTrpAsnPheThr : 167 8113 bp { !}||||||:!! !!:!: |||!..||| !! ++{er}LeuGlyHisProValLeuTrpThrPhePro+ 28787 : .....................ag{CT}CTAGGCCACCCTGTGCTCTGGACATTTCCTg : 20645 168 : >>>> Target Intron 18 >>>> LysPheLeuIleAspLysAsnGlyCysVa : 176 14881 bp ! !|||:!! ! :::|||||||| + ++AlaCysLeuLeuThrCysSerGlyCysVa 20644 : t..........................agGCCTGCCTTCTCACTTGCTCGGGCTGTGT : 5737 177 : l : 176 | l 5736 : g : 5735 vulgar: SPP00000007_1.0 0 176 . NIVO01030317.1:subseq(555646,1182752) 546062 5734 - 152 M 7 21 S 0 2 5 0 2 I 0 68912 3 0 2 S 1 1 M 1 3 5 0 2 I 0 9658 3 0 2 M 5 15 S 0 2 5 0 2 I 0 616 3 0 2 S 1 1 M 1 3 S 0 1 5 0 2 I 0 25304 3 0 2 S 1 2 M 5 15 5 0 2 I 0 8515 3 0 2 M 21 63 5 0 2 I 0 36613 3 0 2 M 7 21 S 0 1 5 0 2 I 0 149221 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 34096 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 9343 3 0 2 S 1 2 M 28 84 5 0 2 I 0 19412 3 0 2 M 26 78 S 0 1 5 0 2 I 0 21213 3 0 2 S 1 2 M 2 6 S 0 2 5 0 2 I 0 59691 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 30823 3 0 2 S 1 1 M 19 57 S 0 2 5 0 2 I 0 12775 3 0 2 S 1 1 M 2 6 5 0 2 I 0 17406 3 0 2 5 0 2 I 0 13144 3 0 2 M 3 9 S 0 1 5 0 2 I 0 8109 3 0 2 S 1 2 M 10 30 5 0 2 I 0 14877 3 0 2 M 10 30 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 5735 546062 152 - . gene_id 4 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546062 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546062 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 546039 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477120 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477120 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477118 477119 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 467458 477119 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 467458 467459 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 467441 467457 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 467441 467457 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 467439 467440 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 466821 467440 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 466821 466822 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 466816 466820 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 466816 466820 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 466814 466815 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 441508 466815 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 441508 441509 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 441491 441507 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 441491 441507 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 441489 441490 . - . intron_id 5 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 432972 441490 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 432972 432973 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 432909 432971 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 432909 432971 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 432907 432908 . - . intron_id 6 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 396292 432908 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 396292 396293 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 396270 396291 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 396270 396291 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 396268 396269 . - . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247045 396269 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247045 247046 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247015 247044 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247015 247044 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247013 247014 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 212915 247014 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 212915 212916 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 212891 212914 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 212891 212914 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 212889 212890 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 203544 212890 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 203544 203545 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 203458 203543 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 203458 203543 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 203456 203457 . - . intron_id 10 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 184042 203457 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 184042 184043 . - . intron_id 9 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 183963 184041 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 183963 184041 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 183961 183962 . - . intron_id 11 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 162746 183962 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 162746 162747 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 162736 162745 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 162736 162745 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 162734 162735 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 103041 162735 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 103041 103042 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 103032 103040 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 103032 103040 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 103030 103031 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 72205 103031 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 72205 72206 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 72145 72204 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 72145 72204 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 72143 72144 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 59366 72144 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 59366 59367 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 59359 59365 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 59359 59365 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 59357 59358 . - . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 59358 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 28801 41948 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 28801 28802 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 28791 28800 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 28791 28800 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 28789 28790 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 20678 28790 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 20678 20679 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 20646 20677 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 20646 20677 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 20644 20645 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 5765 20645 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 5765 5766 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 5735 5764 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 5735 5764 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 5735 546062 152 - . alignment_id 4 ; Query SPP00000007_1.0 ; Align 546063 1 21 ; Align 477123 9 3 ; Align 467458 10 15 ; Align 466820 16 3 ; Align 441506 18 15 ; Align 432972 23 63 ; Align 396292 44 21 ; Align 247043 52 27 ; Align 212913 62 21 ; Align 203542 70 84 ; Align 184042 98 78 ; Align 162744 125 6 ; Align 103040 128 6 ; Align 72204 131 57 ; Align 59365 151 6 ; Align 28801 153 9 ; Align 20676 157 30 ; Align 5765 167 30 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 143 Query range: 0 -> 195 Target range: 1128332 -> 80522 1 : MetSerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAlaLe : 19 |||::!! !||| !! ! !!! !:!!!..||||||! !! !|||||| !! MetThrCysGlyAlaArgThrGlyProValSerProAlaArgArgCysGlyArgCy 1128332 : atgacCTGCGGGGCACGGACGGGGCCCGTCAGCCCGGCACGGCGATGTGGGCGCTG : 1128278 20 : uAlaAlaProGlyLeuAlaGly >>>> Target Intron 1 >>>> ThrMe : 28 ! !|||||| !! !||| ! 3275 bp |||:: sArgAlaProArgGluAlaGln++ ++ThrLe 1128277 : CAGGGCTCCACGGGAGGCCCAGgt.........................agaccct : 1124976 29 : tCysAlaSerArgAspAspTrpArgCysAlaArgSerMetHisGlu >>>> Tar : 44 : ...::: ::: ||| |||... |||::: uMetLeuGlyLysIleGluGlyArgSerArgArgGlyArgHisGln++ 1124975 : gatgctgggcaagattgaaggcaggagcagaaggggacggcaccaggt........ : 1124926 45 : get Intron 2 >>>> PheSerAlaLysAspIleAspGlyHisMetValAsnL : 56 48399 bp !||||||:!!...:!: ||||||:!: !.!.| ++ProSerAlaGlnSerLeuProGlyHisValHisGlnL 1124925 : .................agCCCTCAGCTCAGTCACTGCCAGGCCATGTTCATCAGC : 1076493 57 : euAsp{Ly} >>>> Target Intron 3 >>>> {s}TyrArgGlyPheVal : 63 || {!!} 55424 bp {.}!::|||||||||!.! euArg{As}+- ++{n}TrpArgGlyPheAla 1076492 : TTAGA{AA}ga.........................ag{C}TGGAGGGGATTTGCC : 1021048 64 : CysIle{Va} >>>> Target Intron 4 >>>> {l}Thr >>>> Targ : 68 ||| !{ } 44279 bp {!} ! CysGln{Ly}++ ++{s}Tyr++ 1021047 : TGTCAA{AA}gt.........................ag{G}TATgt......... : 976752 69 : et Intron 5 >>>> AsnValAlaSerGlnUnkGlyLysThrGluValAsnTy : 80 19645 bp ||| !!.!||| ||| ||| !:!!::: ++AsnArgGlySerAlaLeuGlyValThrTrpIleSerGl 976751 : ................agAATAGAGGCTCGGCCCTGGGAGTCACATGGATTTCGGG : 957073 81 : rThrGlnLeuValAsp >>>> Target Intron 6 >>>> LeuHisAlaAr : 89 !|||||| !..!!!: 21768 bp :!!.!.|||! yThrGlnGlyThrGlu++ ++ValGluAlaPr 957072 : CACCCAGGGGACAGAAgt.........................agGTCGAGGCACC : 935278 90 : gTyrAlaGlu<->CysGlyLeuArgIleLeuAlaPheProCysAsnGlnPhe{Gl} : 106 ! !.!!!: |||||||||||| !! !..!.!.||| !...:!!|||{||} oValGlyAspAlaCysGlyLeuArgAlaArgCysLeuProIleArgGluPhe{Gl} 935277 : AGTGGGTGACGCCTGTGGACTCAGAGCTCGTTGTCTGCCCATCCGGGAATTC{GG} : 935224 107 : >>>> Target Intron 7 >>>> {y}LysGlnGluProGlySerAsn{G} : 114 105013 bp {|}:!: ||||||:::{!} ++ ++{y}GlnLeuThrGlyGlySerAsp{A} 935223 : gt.........................ag{G}CAactcactggtggctcagat{g} : 830187 115 : >>>> Target Intron 8 >>>> {lu}GluIleLysGluPheAlaAla : 122 102835 bp {!:}!!: !||| !.!.||| ! ++ ++{sp}AspSerLysMetLeuAlaArg++ 830186 : gt.........................ag{AC}GATTCCAAAATGCTGGCTAGAgt : 727328 123 : >>>> Target Intron 9 >>>> GlyTyrAsnValLysPheAspMetPheSe : 131 174933 bp |||:::||| :::... :::.. ++GlyTrpAsnProArgLeuProArgTrpGl 727327 : .........................agggatggaacccgcgtctcccgcgttggca : 552368 132 : r >>>> Target Intron 10 >>>> >>>> Target Intron 11 > : 132 . 28494 bp 48538 bp n++ ++++ 552367 : ggt..........................aggt....................... : 523869 133 : >>> LysIleCysValAsnGlyAsp{A} >>>> Target Intron 12 >>> : 139 ! :!:||||||::!..!|||{ } 116646 bp ++HisValCysValSerSerAsp{A}++ 523868 : ...agCACGTGTGTGTCTCTTCGGAC{C}gt......................... : 475309 140 : > {sp}AlaHisPro{L} >>>> Target Intron 13 >>>> {eu}Trp : 144 { !}:!!||||||{!} 55778 bp { !}||| ++{rg}SerHisPro{A}++ ++{rg}Trp 475308 : .ag{GC}TCTCATCCC{C}gt..........................ag{GC}TGG : 302873 145 : LysTrpMet >>>> Target Intron 14 >>>> LysIleGlnProLysGl : 153 !..|||:!! 4649 bp !:!|||||||||!..|| SerTrpLeu-+ ++ArgIleGlnProSerGl 302872 : AGCTGGCTGct..........................agAGAATCCAGCCGAGTGG : 298197 154 : yLys{G} >>>> Target Intron 15 >>>> {ly}IleLeuGlyAsnAla : 160 |:!!{|} 24438 bp {||}! !||| !!:! !! yGln{G}++ ++{ly}SerLeuThrSerPro 298196 : ACAA{G}gt..........................ag{GT}AGCCTCACCAGCCCC : 273738 161 : IleLysTrp >>>> Target Intron 16 >>>> AsnPheThrLysPheLe : 169 ! ! !||| 26682 bp ! !..!! || ThrLeuTrp++ ++ValThrValThrThrLe 273737 : ACCCTGTGGgt..........................agGTTACTGTGACCACACT : 247029 170 : uIleAspLysAsn >>>> Target Intron 17 >>>> GlyCysVal >> : 177 ||||..!|||.!. 35529 bp |||||| ! uIleSerLysGlu++ ++GlyCys***++ 247028 : GATAAGCAAAGAGgt..........................agGGATGCTAGgt.. : 211474 178 : >> Target Intron 18 >>>> ValLysArgTyrGlyProMet >>>> Ta : 184 37179 bp !.! !..! ||||||::: ++AlaProGluLeuGlyProLeu++ 211473 : ........................agGCCCCAGAGCTAGGACccttggt....... : 174274 185 : rget Intron 19 >>>> GluGlu >>>> Target Intron 20 >>>> : 186 18147 bp |||::: 75571 bp ++GluLys++ + 174273 : ...................aggaaaaggt..........................a : 80554 187 : ProLeuValIleGluLysAspLeuProHis : 195 ||| !|||:!!..! !||||||||| +ProThrValLeuArgGlyThrLeuProHis 80553 : gCCCACTGTCCTCAGAGGCACCCTTCCCCAC : 80523 vulgar: SPP00000007_1.0 0 195 . NIVO01030317.1:subseq(555646,1182752) 1128332 80522 - 143 M 26 78 5 0 2 I 0 3271 3 0 2 M 17 51 5 0 2 I 0 48395 3 0 2 M 14 42 S 0 2 5 0 2 I 0 55420 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 44275 3 0 2 S 1 1 M 1 3 5 0 2 I 0 19641 3 0 2 M 18 54 5 0 2 I 0 21764 3 0 2 M 7 21 G 0 3 M 13 39 S 0 2 5 0 2 I 0 105009 3 0 2 S 1 1 M 7 21 S 0 1 5 0 2 I 0 102831 3 0 2 S 1 2 M 7 21 5 0 2 I 0 174929 3 0 2 M 10 30 5 0 2 I 0 28490 3 0 2 5 0 2 I 0 48534 3 0 2 M 7 21 S 0 1 5 0 2 I 0 116642 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 55774 3 0 2 S 1 2 M 4 12 5 0 2 I 0 4645 3 0 2 M 7 21 S 0 1 5 0 2 I 0 24434 3 0 2 S 1 2 M 8 24 5 0 2 I 0 26678 3 0 2 M 10 30 5 0 2 I 0 35525 3 0 2 M 3 9 5 0 2 I 0 37175 3 0 2 M 7 21 5 0 2 I 0 18143 3 0 2 M 2 6 5 0 2 I 0 75567 3 0 2 M 10 30 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 80523 1128332 143 - . gene_id 5 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1128255 1128332 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1128255 1128332 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1128253 1128254 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1124980 1128254 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1124980 1124981 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1124929 1124979 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1124929 1124979 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1124927 1124928 . - . intron_id 2 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1076530 1124928 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1076530 1076531 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1076486 1076529 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1076486 1076529 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1076484 1076485 . - . intron_id 3 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1021062 1076485 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1021062 1021063 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1021038 1021061 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1021038 1021061 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1021036 1021037 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1021037 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 976755 976758 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 976755 976758 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976753 976754 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 957110 976754 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 957110 957111 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 957056 957109 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 957056 957109 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 957054 957055 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 935288 957055 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 935288 935289 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 935223 935287 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 935223 935287 . - . insertions 3 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 935221 935222 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 830210 935222 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 830210 830211 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 830187 830209 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 830187 830209 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 830185 830186 . - . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 727352 830186 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 727352 727353 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 727329 727351 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 727329 727351 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 727327 727328 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 552396 727328 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 552396 552397 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 552366 552395 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 552366 552395 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 552364 552365 . - . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 552365 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 475334 523871 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 475334 475335 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 475312 475333 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 475312 475333 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 475310 475311 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 358666 475311 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 358666 358667 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 358654 358665 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 358654 358665 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 358652 358653 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 302876 358653 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 302876 302877 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 302862 302875 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 302862 302875 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 302860 302861 . - . intron_id 14 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 298213 302861 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 298213 298214 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 298191 298212 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 298191 298212 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 298189 298190 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 273753 298190 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 273753 273754 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 273727 273752 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 273727 273752 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 273725 273726 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247045 273726 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247045 247046 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247015 247044 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247015 247044 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247013 247014 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 211486 247014 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 211486 211487 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 211477 211485 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 211477 211485 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 211475 211476 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 174298 211476 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 174298 174299 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 174277 174297 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 174277 174297 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 174275 174276 . - . intron_id 19 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 156130 174276 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 156130 156131 . - . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 156124 156129 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 156124 156129 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 156122 156123 . - . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 80553 156123 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 80553 80554 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 80523 80552 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 80523 80552 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 80523 1128332 143 - . alignment_id 5 ; Query SPP00000007_1.0 ; Align 1128333 1 78 ; Align 1124980 27 51 ; Align 1076530 44 42 ; Align 1021061 59 21 ; Align 976758 67 3 ; Align 957110 68 54 ; Align 935288 86 21 ; Align 935264 93 39 ; Align 830209 107 21 ; Align 727350 115 21 ; Align 552396 122 30 ; Align 475334 132 21 ; Align 358664 140 9 ; Align 302874 144 12 ; Align 298213 148 21 ; Align 273751 156 24 ; Align 247045 164 30 ; Align 211486 174 9 ; Align 174298 177 21 ; Align 156130 184 6 ; Align 80553 186 30 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 142 Query range: 12 -> 195 Target range: 1142708 -> 6423 13 : AlaLeuLeuCysGlyAlaLeuAlaAlaProGly >>>> Target Intron 1 : 24 |||! !||||||||||||||||||..! !! ! 30283 bp AlaArgLeuCysGlyAlaLeuAlaCysSerPro++ 1142708 : GCACGACTGTGCGGGGCTCTGGCTTGTTCTCCAgt..................... : 1142673 25 : >>>> LeuAlaGlyThrMetCysAlaSerArgAspAspTrpArgCysAlaArgSe : 40 ! !:!!||| |||:::...|||.!.! ! !!:!... !!..! ! ++ArgSerGlyGlnHisCysSerGlnArgGlnAlaMetGlnAlaProGluAr 1142672 : ....agCGCTCAgggcagcactgttcacaacgcCAAGCCATGCAAGCACCTGAACG : 1112344 41 : rMetHisGluPheSerAlaLysAspIleAspGlyHis{M} >>>> Target In : 53 ! |||..! !!!! !!:!!!::!:||| !!|||{ } 139680 gHisHisArgArgThrAspArgGluValAspArgHis{A}++ 1112343 : TCATCACAGACGGACAGACAGAGAGGTGGACAGACAC{G}gt.............. : 1112302 54 : tron 2 >>>> {et}ValAsnLeuAspLysTyrArgGlyPheValCysIleVal : 66 bp { }:::||||||...:::::::::|||... ||| -+{la}LeuAsnLeuSerGlnHisGlnGlyLeuPhe******Val 1112301 : ...........tg{cc}ctcaatctttcccagcatcagggtcttttctaatgagtc : 972586 67 : ThrAsnValAlaSer >>>> Target Intron 3 >>>> Gln{Un} >>> : 73 ::::::::: ... 17019 bp !{ } SerSerLeuHisGln++ ++Gly{Me}++ 972585 : agttctttgcatcaggt.........................agGGA{AT}gt... : 955542 74 : > Target Intron 4 >>>> {k}GlyLysThrGluValAsnTyrThrGln{L : 83 1560 bp { }...:::|||:::|||::: ||| { ++{t}AsnGlnThrGlnValSerCysThrGly{S 955541 : ......................ag{g}aatcaaacccaggtctcctgcactggc{a : 953956 84 : e} >>>> Target Intron 5 >>>> {u}ValAspLeuHisAlaArgTyrA : 91 } 42593 bp {!}::! !.!.|||..!!:!: e}++ ++{r}LeuArgAlaGluAlaGluPheS 953955 : g}gt.........................ag{c}ctTCGGGCCGAAGCAGAGTTCT : 911339 92 : laGluCys >>>> Target Intron 6 >>>> Gly{Le} >>>> Targe : 95 !!:!!... 12858 bp |||{ } 1 erGlnAla++ ++Gly{Ly}++ 911338 : CGCAGGCGgt.........................agGGA{AA}gt.......... : 898465 96 : t Intron 7 >>>> {u}ArgIleLeuAlaPheProCysAsn{Gl} >>>> T : 104 4077 bp {!}|||! :!!::!|||||| ...{!:} ++{s}ArgThrMetSerPheProGluGly{Ar}++ 898464 : ...............ag{A}AGGACGATGAGCTTTCCAGAAGGA{CG}gt...... : 884361 105 : arget Intron 8 >>>> {n}PheGlyLysGlnGluProGlySer >>>> T : 113 41789 bp {!}||||||:::.!.:!! !|||... ++{g}PheGlyArgAsnGlnLeuGlyGlu++ 884360 : ...................ag{G}TTTGGCCGTAACCAATTGGGGGAggt...... : 842547 114 : arget Intron 9 >>>> AsnGluGluIleLys >>>> Target Intron : 118 45606 bp !!.... !|||||| 138581 bp ++LysSerThrIleLys++ 842546 : ...................agAAAAGCACGATAAAGgt.................. : 796926 119 : 10 >>>> GluPheAlaAlaGlyTyrAsnValLys{P} >>>> Target In : 127 !!:!!.!.! !|||! !..||||||{ } 80591 ++AspLeuValLysGlyLeuArgValLys{G}++ 796925 : ........agGATTTGGTCAAAGGGTTGAGGGTAAAA{C}gt.............. : 658317 128 : tron 11 >>>> {he} >>>> Target Intron 12 >>>> >>>> T : 128 bp { } 53855 bp ++{ln}++ ++++ 658316 : ............ag{AG}gt..........................aggt...... : 523869 129 : arget Intron 13 >>>> AspMetPheSerLysIleCysVal >>>> Tar : 136 35193 bp ! !! |||||||||! ! ! ++GlySerPheSerLysArgGluPro++ 523868 : ....................agGGCAGCTTTTCCAAGAGAGAACCCgt........ : 488652 137 : get Intron 14 >>>> AsnGlyAspAsp >>>> Target Intron 15 : 140 11531 bp !.!|||!!:.!. 34099 bp ++ThrGlyGluGln++ 488651 : ..................agACTGGTGAACAAgt...................... : 477109 141 : >>>> AlaHisProLeuTrpLys >>>> Target Intron 16 >>>> Tr : 146 |||!.!||||||||| ! 41321 bp || ++AlaArgProLeuTrpPro++ -+Tr 477108 : ....agGCTCGTCCACTCTGGCCGgt..........................ggTG : 401673 147 : pMetLysIle{Gl} >>>> Target Intron 17 >>>> {n}ProLysGly : 153 |:!: !:!!{::} 844 bp {!}||| !||| pValLeuLeu{Ar}++ ++{g}ProGlyGly 401672 : GGTCTTACTC{AG}gt..........................ag{G}CCTGGAGGG : 400808 154 : LysGlyIle{Le} >>>> Target Intron 18 >>>> {u}GlyAsnAlaI : 161 ...|||:!:{ } 102815 bp {!}|||...:!!| SerGlyLeu{Ar}++ ++{g}GlyArgSerI 400807 : TCTGGTCTG{AG}gt..........................ag{G}GGGCGGTCCA : 297969 162 : leLysTrpAsnPheThrLysPhe{Le} >>>> Target Intron 19 >>>> : 169 || !||| !!:!{ } 24090 bp leAlaThrTrpAsnThrAlaTyr{Ar}++ + 297968 : TAGCCACCTGGAACACAGCATAC{AG}gt..........................a : 273855 170 : {u}Ile{A} >>>> Target Intron 20 >>>> {sp}LysAsnGlyCys : 175 {!} !{!} 57653 bp { !} !.!|||||| +{g}Ala{G}++ ++{ly}ProThrGlyCys 273854 : g{A}GCT{G}gt..........................ag{GT}CCCACTGGCTGC : 216184 176 : ValValLysArgTyrGlyProMet{Gl} >>>> Target Intron 21 >>>> : 184 :!! ! ! ||||||! !{..} 151815 bp IleArgAlaAlaGlyGlyProArg{Se}++ 216183 : ATCAGAGCCGCTGGAGGGCCCAGG{AG}gt.......................... : 216153 185 : {u}GluProLeuValIleGluLysAsp >>>> Target Intron 22 >>> : 193 {.} ||||||:!!|||... ! .!. 22367 bp ++{r}ThrProLeuLeuIleSerTyrGln++ 216152 : ag{C}ACCCCACTTCTCATCAGCTACCAGgt......................... : 64313 194 : > >>>> Target Intron 23 >>>> LeuProHis : 195 35516 bp ! !|||||| ++++ ++TyrProHis 64312 : .aggt..........................agTACCCCCAC : 6424 vulgar: SPP00000007_1.0 12 195 . NIVO01030317.1:subseq(555646,1182752) 1142708 6423 - 142 M 11 33 5 0 2 I 0 30279 3 0 2 M 29 87 S 0 1 5 0 2 I 0 139676 3 0 2 S 1 2 M 18 54 5 0 2 I 0 17015 3 0 2 M 1 3 S 0 2 5 0 2 I 0 1556 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 42589 3 0 2 S 1 1 M 10 30 5 0 2 I 0 12854 3 0 2 M 1 3 S 0 2 5 0 2 I 0 14073 3 0 2 S 1 1 M 8 24 S 0 2 5 0 2 I 0 41785 3 0 2 S 1 1 M 8 24 5 0 2 I 0 45602 3 0 2 M 5 15 5 0 2 I 0 138577 3 0 2 M 9 27 S 0 1 5 0 2 I 0 80587 3 0 2 S 1 2 5 0 2 I 0 53851 3 0 2 5 0 2 I 0 35189 3 0 2 M 8 24 5 0 2 I 0 11527 3 0 2 M 4 12 5 0 2 I 0 34095 3 0 2 M 6 18 5 0 2 I 0 41317 3 0 2 M 4 12 S 0 2 5 0 2 I 0 840 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 102811 3 0 2 S 1 1 M 11 33 S 0 2 5 0 2 I 0 24086 3 0 2 S 1 1 M 1 3 S 0 1 5 0 2 I 0 57649 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 151811 3 0 2 S 1 1 M 8 24 5 0 2 I 0 22363 3 0 2 5 0 2 I 0 35512 3 0 2 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 6424 1142708 142 - . gene_id 6 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1142676 1142708 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1142676 1142708 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1142674 1142675 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1112393 1142675 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1112393 1112394 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1112305 1112392 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1112305 1112392 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1112303 1112304 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 972625 1112304 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 972625 972626 . - . intron_id 1 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 972569 972624 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 972569 972624 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 972567 972568 . - . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 955550 972568 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 955550 955551 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 955545 955549 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 955545 955549 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 955543 955544 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 953985 955544 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 953985 953986 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 953955 953984 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 953955 953984 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 953953 953954 . - . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 911362 953954 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 911362 911363 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 911331 911361 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 911331 911361 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 911329 911330 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 898473 911330 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 898473 898474 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 898468 898472 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 898468 898472 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 898466 898467 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 884391 898467 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 884391 884392 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 884364 884390 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 884364 884390 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 884362 884363 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 842575 884363 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 842575 842576 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 842550 842574 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 842550 842574 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 842548 842549 . - . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 796944 842549 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 796944 796945 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 796929 796943 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 796929 796943 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 796927 796928 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 658348 796928 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 658348 658349 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 658320 658347 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 658320 658347 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 658318 658319 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 577729 658319 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 577729 577730 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 577727 577728 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 577727 577728 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 577725 577726 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 577726 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 488679 523871 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 488679 488680 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 488655 488678 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 488655 488678 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 488653 488654 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 488654 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 443013 477111 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 443013 443014 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 442995 443012 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 442995 443012 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 442993 442994 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 401674 442994 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 401674 401675 . - . intron_id 15 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 401660 401673 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 401660 401673 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 401658 401659 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 400816 401659 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 400816 400817 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 400795 400815 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 400795 400815 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 400793 400794 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297980 400794 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297980 297981 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297944 297979 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297944 297979 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297942 297943 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 273854 297943 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 273854 273855 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 273849 273853 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 273849 273853 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 273847 273848 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 216196 273848 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 216196 216197 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 216156 216195 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 216156 216195 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 216154 216155 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64341 216155 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64341 64342 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64316 64340 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64316 64340 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64314 64315 . - . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 64315 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 6433 41948 . - . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 6433 6434 . - . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 6424 6432 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 6424 6432 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 6424 1142708 142 - . alignment_id 6 ; Query SPP00000007_1.0 ; Align 1142709 13 33 ; Align 1112393 24 87 ; Align 972623 54 54 ; Align 955550 72 3 ; Align 953984 74 27 ; Align 911361 84 30 ; Align 898473 94 3 ; Align 884390 96 24 ; Align 842574 105 24 ; Align 796944 113 15 ; Align 658348 118 27 ; Align 488679 128 24 ; Align 477124 136 12 ; Align 443013 140 18 ; Align 401674 146 12 ; Align 400815 151 18 ; Align 297979 158 33 ; Align 273853 170 3 ; Align 216194 172 36 ; Align 64340 185 24 ; Align 6433 193 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 139 Query range: 1 -> 195 Target range: 1134554 -> 7491 2 : SerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCys >>>> Targ : 17 :!! !||||||:!! |||! !! !|||||||||||| ! ! AlaAlaGlyArgValThrArgProProLysProAlaLeuThrArg++ 1134554 : GCGGCCGGGAGGGTCACACGTCCACCCAAACCCGCTCTGACCAGGgt......... : 1134507 18 : et Intron 1 >>>> GlyAlaLeuAlaAlaPro{G} >>>> Target Int : 23 90073 bp |||!.!:!!.!!!.!|||{|} 67659 ++GlyValMetThrValPro{G}++ 1134506 : ................agGGGGTGATGACTGTACCC{G}gt............... : 1044415 24 : ron 2 >>>> {ly}{Le} >>>> Target Intron 3 >>>> {u} >> : 25 bp {||}{:!} 18291 bp {!} ++{ly}{Me}++ ++{t}++ 1044414 : ..........ag{GT}{AT}gt.........................ag{G}gt.. : 958460 26 : >> Target Intron 4 >>>> AlaGly >>>> Target Intron 5 >> : 27 148579 bp ::!||| 6507 bp ++SerGly++ 958459 : .......................agAGCGGTgt....................... : 809875 28 : >> ThrMetCysAlaSerArgAspAspTrpArgCysAlaArgSerMetHisGluP : 44 ...|||::: ||| ||| ||| |||... ++GluAsnSerMetGlyArgGluAlaTrpSerCysArgArgValArgHisSer* 809874 : ..aggaaaattccatgggcagagaagcctggagttgcaggagagtcaggcacagct : 803319 45 : heSerAlaLysAspIleAsp{Gl} >>>> Target Intron 6 >>>> {y} : 51 ::!||| |||::: {||} 81576 bp {|} **ThrAlaValAspMetThr{Gl}+- ++{y} 803318 : gaacAGCagtggacatgact{gg}gc.........................ag{g} : 721720 52 : HisMetValAsnLeuAspLysTyrArgGlyPheValCysIleValThr{A} >>> : 68 ...::::::::: :::|||:::||| ::: ::! !|||{.} ArgLeuLeuHis***LeuArgTyrGlnGlySerLeuGlnValTyrThr{G}-+ 721719 : cggcttcttcactagctgaggtaccagggaagcctgcaggtATATACA{G}ct... : 721668 69 : > Target Intron 7 >>>> {sn}ValAlaSerGln >>>> Target In : 73 1090 bp {!.}|||||||||:!! 48544 ++{lu}ValAlaSerGlu++ 721667 : ......................ag{AA}GTCGCCTCGGAGgt.............. : 720564 74 : tron 8 >>>> UnkGlyLys >>>> Target Intron 9 >>>> ThrGl : 77 bp |||||| 107941 bp |||:! -+LysGlyLys++ ++ThrGl 720563 : ...........tgaaaggaAAGgt.........................agACTCA : 564069 78 : uValAsnTyrThr >>>> Target Intron 10 >>>> GlnLeuVal >> : 85 !:!!...||| ! 18006 bp :!!||| ! nIleGlyTyrGln++ ++GluLeuGln++ 564068 : GATTGGGTACCAGgt..........................agGAGCTGCAGgt.. : 546037 86 : >> Target Intron 11 >>>> >>>> Target Intron 12 >>>> : 85 22168 bp 6749 bp ++++ ++ 546036 : ........................aggt..........................ag : 517124 86 : AspLeuHisAlaArgTyr{A} >>>> Target Intron 13 >>>> {la}G : 92 ! :!! !|||!!!:!!{|} 17397 bp {||}| LysIleSerAlaSerHis{A}+- ++{la}G 517123 : aAGATCTCTGCTAGTCAT{G}gg..........................ag{CA}G : 499704 93 : luCysGlyLeuArg{Il} >>>> Target Intron 14 >>>> {e}LeuAl : 99 ||! ||| !!:!{ } 11009 bp { } !|| luSerGlyThrGln{Gl}++ ++{y}AlaAl 499703 : AATCAGGGACTCAA{GG}gt..........................ag{G}GCAGC : 488674 100 : aPheProCysAsnGln{Ph} >>>> Target Intron 15 >>>> {e}Gly : 106 ||||||| ! .!..!.{ } 55683 bp { }||| aPheProArgGluAsn{Pr}++ ++{o}Gly 488673 : TTTTCCAAGAGAGAAC{CC}gt..........................ag{g}gga : 432970 107 : LysGlnGluProGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyrAsnVa : 125 ... :::||| ...... ::::::... ...|||...|||::: .. SerLeuGlnProArgAspGlnThrGlnValSerGlyIleAlaGlyGlyPhePheTh 432969 : tctctgcaacccagggatcaaacccaggtctctggcattgcaggcggcttcttcac : 432913 126 : l{Ly} >>>> Target Intron 16 >>>> {s}PheAspMetPheSer{L} : 132 .{!.} 84567 bp {.} :::||||||{!} r{Se}-+ ++{r}ValThrValPheSer{S} 432912 : c{ag}ct..........................ag{c}gtaacagtcttttcc{a} : 348325 133 : >>>> Target Intron 17 >>>> {ys}IleCysValAsnGlyAspAspA : 140 101280 bp {..} !|||! !:!! !||| ! ++ ++{er}TyrCysAspHisThrAspLysG 348324 : gt..........................ag{GT}TACTGTGACCACACTGATAAGC : 247021 141 : laHis{P} >>>> Target Intron 18 >>>> {ro}LeuTrpLys >>> : 146 !...{ } 62789 bp {!!}||||||:!! lnArg{A}++ ++{la}LeuTrpGln++ 247020 : AAAGA{G}gt..........................ag{CC}CTGTGGCAGgt... : 184212 147 : > Target Intron 19 >>>> TrpMetLysIleGlnProLysGlyLys{Gl} : 155 47150 bp ! !||| ||| !||||||:::{ !} ++SerArgLysAlaGlnAspLysGlyArg{Ar} 184211 : .......................agAGTCGGAAGGCGCAGGACAAGGGCCGC{CG} : 137037 156 : >>>> Target Intron 20 >>>> {y}IleLeuGlyAsn{Al} >>>> : 160 31197 bp {!}::: |||:::{::} ++ ++{g}MetSerGlySer{Se}++ 137036 : gt..........................ag{g}atgtctggttct{ag}gt..... : 105821 161 : Target Intron 21 >>>> {a}IleLysTrpAsnPheThrLysPheLeuIle : 170 8819 bp {!}|||...|||:::... ||||||::: +-{r}IleSerTrpSerLeuLeuLysPheMetSer 105820 : .....................ac{c}atctcctggagtttgctcaaattcatgtcc : 96976 171 : AspLysAsn >>>> Target Intron 22 >>>> GlyCysValValLys : 179 :::::: 9509 bp ||||||:!! !:!! IleGluSer++ ++GlyCysLeuSerGln++ 96975 : attgagtcggt..........................agGGGTGCCTGAGTCAGgt : 87440 180 : >>>> Target Intron 23 >>>> ArgTyrGlyPro{Me} >>>> Targe : 183 26204 bp |||||| !|||{:!} 5 ++ArgTyrGlnPro{Le}++ 87439 : ..........................agAGATACCAGCCC{CT}gt.......... : 61220 184 : t Intron 24 >>>> {t}GluGluProLeuValIleGluLysAspLeuProHi : 195 3694 bp {:}|||..!||| !:!!! !! ! ! !:!!||||| ++{u}GluArgProGluLeuThrValAspThrIleProHi 61219 : ................ag{T}GAGCGGCCTGAGCTCACCGTGGATACCATTCCCCA : 7494 196 : s : 195 | s 7493 : C : 7492 vulgar: SPP00000007_1.0 1 195 . NIVO01030317.1:subseq(555646,1182752) 1134554 7491 - 139 M 15 45 5 0 2 I 0 90069 3 0 2 M 6 18 S 0 1 5 0 2 I 0 67655 3 0 2 S 1 4 5 0 2 I 0 18287 3 0 2 S 1 1 5 0 2 I 0 148575 3 0 2 M 2 6 5 0 2 I 0 6503 3 0 2 M 24 72 S 0 2 5 0 2 I 0 81572 3 0 2 S 1 1 M 16 48 S 0 1 5 0 2 I 0 1086 3 0 2 S 1 2 M 4 12 5 0 2 I 0 48540 3 0 2 M 3 9 5 0 2 I 0 107937 3 0 2 M 6 18 5 0 2 I 0 18002 3 0 2 M 3 9 5 0 2 I 0 22164 3 0 2 5 0 2 I 0 6745 3 0 2 M 6 18 S 0 1 5 0 2 I 0 17393 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 11005 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 55679 3 0 2 S 1 1 M 20 60 S 0 2 5 0 2 I 0 84563 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 101276 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 62785 3 0 2 S 1 2 M 3 9 5 0 2 I 0 47146 3 0 2 M 9 27 S 0 2 5 0 2 I 0 31193 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 8815 3 0 2 S 1 1 M 13 39 5 0 2 I 0 9505 3 0 2 M 5 15 5 0 2 I 0 26200 3 0 2 M 4 12 S 0 2 5 0 2 I 0 53690 3 0 2 S 1 1 M 12 36 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 7492 1134554 139 - . gene_id 7 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1134510 1134554 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1134510 1134554 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1134508 1134509 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1044437 1134509 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1044437 1044438 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1044418 1044436 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1044418 1044436 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1044416 1044417 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1044417 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 976755 976758 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 976755 976758 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976753 976754 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 958464 976754 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 958464 958465 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 958463 958463 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 958463 958463 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 958461 958462 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 809884 958462 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 809884 809885 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 809878 809883 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 809878 809883 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 809876 809877 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 803371 809877 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 803371 803372 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 803297 803370 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 803297 803370 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 803295 803296 . - . intron_id 6 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 721721 803296 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 721721 721722 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 721671 721720 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 721671 721720 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 721669 721670 . - . intron_id 7 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 720581 721670 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 720581 720582 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 720567 720580 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 720567 720580 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 720565 720566 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 672023 720566 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 672023 672024 . - . intron_id 7 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 672014 672022 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 672014 672022 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 672012 672013 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 564073 672013 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 564073 564074 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 564055 564072 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 564055 564072 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 564053 564054 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 564054 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 546039 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 517123 523871 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 517123 517124 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 517104 517122 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 517104 517122 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 517102 517103 . - . intron_id 13 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 499707 517103 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 499707 499708 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 499688 499706 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 499688 499706 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 499686 499687 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 488679 499687 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 488679 488680 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 488655 488678 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 488655 488678 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 488653 488654 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 432972 488654 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 432972 432973 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 432909 432971 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 432909 432971 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 432907 432908 . - . intron_id 16 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 348342 432908 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 348342 348343 . - . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 348325 348341 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 348325 348341 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 348323 348324 . - . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247045 348324 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247045 247046 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247015 247044 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247015 247044 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247013 247014 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 184226 247014 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 184226 184227 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 184215 184225 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 184215 184225 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 184213 184214 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 137065 184214 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 137065 137066 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 137036 137064 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 137036 137064 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 137034 137035 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 105839 137035 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 105839 105840 . - . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 105824 105838 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 105824 105838 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 105822 105823 . - . intron_id 21 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 97005 105823 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 97005 97006 . - . intron_id 20 ; splice_site "ac" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 96965 97004 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 96965 97004 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 96963 96964 . - . intron_id 22 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 87456 96964 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 87456 87457 . - . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 87441 87455 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 87441 87455 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 87439 87440 . - . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 61237 87440 . - . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 61237 61238 . - . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 61223 61236 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 61223 61236 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 61221 61222 . - . intron_id 24 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 7529 61222 . - . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 7529 7530 . - . intron_id 23 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 7492 7528 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 7492 7528 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 7492 1134554 139 - . alignment_id 7 ; Query SPP00000007_1.0 ; Align 1134555 2 45 ; Align 1044437 17 18 ; Align 809884 25 6 ; Align 803371 27 72 ; Align 721720 52 48 ; Align 720579 69 12 ; Align 672023 73 9 ; Align 564073 76 18 ; Align 546049 82 9 ; Align 517123 85 18 ; Align 499705 92 15 ; Align 488678 98 21 ; Align 432971 106 60 ; Align 348341 127 15 ; Align 247043 133 27 ; Align 184224 143 9 ; Align 137065 146 27 ; Align 105838 156 12 ; Align 97004 161 39 ; Align 87456 174 15 ; Align 61237 179 12 ; Align 7528 184 36 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 137 Query range: 2 -> 182 Target range: 1044277 -> 6675 3 : LeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeu{C} >>>> Target : 16 :!!|||..!:!!|||! !|||||| ! ! !!:!!! !{ } 276 ValGlyGluValCysIleLeuLeuLeuMetProIleSer{V}++ 1044277 : GTGGGTGAAGTCTGTATCTTATTGTTGATGCCTATATCA{G}gt............ : 1044235 17 : Intron 1 >>>> {ys}GlyAlaLeu{A} >>>> Target Intron 2 >> : 20 45 bp { }||||||:!!{!} 6314 bp ++{al}GlyAlaVal{G}++ 1044234 : .............ag{TA}GGCGCTGTA{G}gt....................... : 1016578 21 : >> {la}AlaProGlyLeuAla >>>> Target Intron 3 >>>> GlyT : 27 {.!} !|||||||||||| 2915 bp |||. ++{ly}LysProGlyLeuAla++ ++GlyA 1016577 : ..ag{GG}AAACCTGGACTTGCGgt.........................agggaa : 1007331 28 : hrMetCysAlaSerArgAsp{A} >>>> Target Intron 4 >>>> {sp} : 34 ..:::|||||| :::{|} 4451 bp {||} snValCysAlaCysValGlu{A}++ ++{sp} 1007330 : acgtgtgtgcatgtgtagaa{g}gt.........................ag{AC} : 1002858 35 : TrpArgCysAlaArgSerMetHisGlu >>>> Target Intron 5 >>>> : 44 |||..! ! |||!!!|||:!! !||| 26071 bp TrpGluGlyAlaSerSerLeuSerGlu++ ++ 1002857 : TGGGAGGGGGCCAGTTCTCTGAGCGAGgt.........................ag : 976760 45 : >>>> Target Intron 6 >>>> PheSerAlaLys{A} >>>> Targe : 48 132886 bp ||| !!:!{|} 4 ++ ++LysSerLeuArg{A}++ 976759 : gt.........................agAAAAGCTTGAGA{G}gt.......... : 843857 49 : t Intron 7 >>>> {sp}IleAspGlyHisMetValAsnLeuAspLys{T} : 59 9920 bp {||}:!!:!!..!:!!:!: !!.!||| !!:!{ } ++{sp}LeuAsnSerAsnLeuProThrLeuPheArg{A}++ 843856 : ...............ag{AT}TTAAACTCAAATCTTCCTACTTTATTTAGA{A}gt : 793906 60 : >>>> Target Intron 8 >>>> {yr}ArgGlyPheValCys{Il} >>>> : 65 24200 bp { }|||||| |||{||} ++{rg}ArgGlyGlyGlyCys{Il}++ 793905 : .........................ag{gg}aggggtgggggatgc{AT}gt.... : 769685 66 : Target Intron 9 >>>> {e}ValThrAsnValAlaSerGlnUnkGly > : 75 163512 bp {|}:!! ! !!.!|||.!!|||... ! ++{e}LeuAspAlaAlaAlaGlyGlnAlaGln++ 769684 : .....................ag{C}CTGGATGCCGCCGCTGGCCAAGCTCAGgt. : 606145 76 : >>> Target Intron 10 >>>> >>>> Target Intron 11 >>>> : 75 82276 bp 16612 bp ++++ + 606144 : .........................aggt..........................a : 507261 76 : LysThrGluValAsnTyrThrGlnLeuVal >>>> Target Intron 12 > : 85 ! ||||||! !::: !|||! |||:!! 92927 bp +ThrThrGluGlySerAlaThrProLeuLeu++ 507260 : gACCACTGAAGGTTCAGCCACACCCTTGCTGgt....................... : 507227 86 : >>> AspLeuHisAlaArgTyrAlaGluCysGlyLeu >>>> Target Intr : 96 ||| !!!.!!.!:!!|||.!.||||||||| 9170 b ++ArgLeuAspGlyHisHisAlaHisCysGlyLeu++ 507226 : ...agAGGCTGGATGGACATCACGCCCATTGCGGGCTGgt................ : 414267 97 : on 13 >>>> Arg{Il} >>>> Target Intron 14 >>>> {e}LeuA : 99 p ..!{!!} 72498 bp {:}||| ++Glu{Me}++ ++{t}LeuL 414266 : ..........agGAG{AT}gt..........................ag{G}CTGA : 332592 100 : laPheProCysAsnGlnPheGly{Ly} >>>> Target Intron 15 >>>> : 107 !.!.|||! !:!:!! ! |||{||} 133699 bp ysLeuPro***SerGluValGly{Ly}++ + 332591 : AGCTGCCTTAAAGTGAAGTAGGG{AA}gt..........................a : 198869 108 : {s}GlnGluProGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyrAsn : 125 {|}:!!!!: ! :::|||:::...|||::::::!!....|||||| ... +{s}GluAspGlyArgThrAsnLysAsnIleArgAspLeuThrAlaGlyGlyGly+ 198868 : g{G}GAAGACGGACggacaaataaaaatatcagagatTTGAcagcagggggtgggg : 198815 126 : >>>> Target Intron 16 >>>> ValLysPheAspMetPheSerLysIle : 133 54380 bp |||:::|||! ! ...... ::: + ++ValArgPheGlyCysLeuGlnGlyVal 198814 : t..........................agGTTCGCTTTGGCtgcctgcagggggtg : 144411 134 : Cys >>>> Target Intron 17 >>>> >>>> Target Intron 18 : 135 102457 bp 18619 bp Gly++ ++++ 144410 : ggggt..........................aggt..................... : 41946 136 : >>>> ValAsnGlyAspAspAlaHisProLeuTrpLysTrpMetLysIleGlnP : 151 :!!:::|||... !! !...||| ! ! ||| ! !:!!! | ++LeuSerGlySerArgAspArgProSerProHisTrpArgAlaLeuProP 41945 : .....agCTGTCAGGCTCACGTGACAGGCCCAGCCCTCATTGGCGGGCGCTTCCCC : 23281 152 : roLysGlyLysGlyIleLeuGly{As} >>>> Target Intron 19 >>>> : 159 ||! !|||||||||:!!! ! !!{..} 5649 bp roThrGlyLysGlyLeuArgArg{Ar}++ + 23280 : CCACAGGAAAAGGCCTCCGGAGG{CG}gt..........................a : 17608 160 : {n} >>>> Target Intron 20 >>>> AlaIleLysTrpAsnPheThrL : 167 {.} 10861 bp !.!:!: ! ||| !.!. !! +{g}++ ++GlyLeuHisTrpLeuLeuProH 17607 : g{G}gt..........................agGGTCTGCACTGGCTCCTGCCCC : 6723 168 : ysPheLeuIleAspLysAsnGlyCysValValLysArgTyrGlyPro : 182 ! |||:!!.!. ! !||| !:!! ! ..! |||||| isGlnLeuLeuGlnValIleGlyLeuIleProValGluGlyGlyPro 6722 : ACCAGCTCCTTCAGGTCATTGGCCTCATACCAGTTGAGGGAGGGCCC : 6676 vulgar: SPP00000007_1.0 2 182 . NIVO01030317.1:subseq(555646,1182752) 1044277 6675 - 137 M 13 39 S 0 1 5 0 2 I 0 27641 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 6310 3 0 2 S 1 2 M 5 15 5 0 2 I 0 2911 3 0 2 M 8 24 S 0 1 5 0 2 I 0 4447 3 0 2 S 1 2 M 9 27 5 0 2 I 0 26067 3 0 2 5 0 2 I 0 132882 3 0 2 M 4 12 S 0 1 5 0 2 I 0 49916 3 0 2 S 1 2 M 10 30 S 0 1 5 0 2 I 0 24196 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 163508 3 0 2 S 1 1 M 9 27 5 0 2 I 0 82272 3 0 2 5 0 2 I 0 16608 3 0 2 M 10 30 5 0 2 I 0 92923 3 0 2 M 11 33 5 0 2 I 0 9166 3 0 2 M 1 3 S 0 2 5 0 2 I 0 72494 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 133695 3 0 2 S 1 1 M 17 51 5 0 2 I 0 54376 3 0 2 M 10 30 5 0 2 I 0 102453 3 0 2 5 0 2 I 0 18615 3 0 2 M 24 72 S 0 2 5 0 2 I 0 5645 3 0 2 S 1 1 5 0 2 I 0 10857 3 0 2 M 23 69 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 6676 1044277 137 - . gene_id 8 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1044238 1044277 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1044238 1044277 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1044236 1044237 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016593 1044237 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016593 1016594 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016592 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016592 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1010267 1016580 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1010267 1010268 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1010250 1010266 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1010250 1010266 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1010248 1010249 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1007335 1010249 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1007335 1007336 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1007310 1007334 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1007310 1007334 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1007308 1007309 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1002859 1007309 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1002859 1002860 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1002830 1002858 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1002830 1002858 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1002828 1002829 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1002829 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 843873 976758 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 843873 843874 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 843860 843872 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 843860 843872 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 843858 843859 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 793940 843859 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 793940 793941 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 793907 793939 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 793907 793939 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 793905 793906 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 769707 793906 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 769707 769708 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 769688 769706 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 769688 769706 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 769686 769687 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 606176 769687 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 606176 606177 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 606148 606175 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 606148 606175 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 606146 606147 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 606147 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 507260 523871 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 507260 507261 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 507230 507259 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 507230 507259 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 507228 507229 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 414303 507229 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 414303 414304 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 414270 414302 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 414270 414302 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 414268 414269 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 405100 414269 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 405100 405101 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 405095 405099 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 405095 405099 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 405093 405094 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 332597 405094 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 332597 332598 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 332567 332596 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 332567 332596 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 332565 332566 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 198868 332566 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 198868 198869 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 198816 198867 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 198816 198867 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 198814 198815 . - . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 144436 198815 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 144436 144437 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 144406 144435 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 144406 144435 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 144404 144405 . - . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 144405 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 23330 41948 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 23330 23331 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 23256 23329 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 23256 23329 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 23254 23255 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 17607 23255 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 17607 17608 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 17606 17606 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 17606 17606 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 17604 17605 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 6745 17605 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 6745 6746 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 6676 6744 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 6676 6744 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 6676 1044277 137 - . alignment_id 8 ; Query SPP00000007_1.0 ; Align 1044278 3 39 ; Align 1016591 17 9 ; Align 1010265 21 15 ; Align 1007335 26 24 ; Align 1002857 35 27 ; Align 843873 44 12 ; Align 793938 49 30 ; Align 769705 60 15 ; Align 606175 66 27 ; Align 507260 75 30 ; Align 414303 85 33 ; Align 405100 96 3 ; Align 332596 98 27 ; Align 198867 108 51 ; Align 144436 125 30 ; Align 23330 135 72 ; Align 6745 160 69 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 135 Query range: 3 -> 190 Target range: 1169194 -> 4693 4 : GlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAla{L} >>>> T : 19 ..!||| ! !|||:!!|||||||||! !! !! ||| !!{ } SerArgSerLeuSerLeuValLysProAlaHisHisSerGlyPro{A}++ 1169194 : TCTAGGAGCCTATCGCTTGTGAAGCCTGCCCACCACTCGGGGCCG{G}gt...... : 1169146 20 : arget Intron 1 >>>> {eu}AlaAlaProGlyLeuAlaGlyThrMetCysA : 30 73521 bp { !} !!|||||| !! ! !||||||:::||| ++{sp}ProAlaProProSerTyrGlyThrLeuCysP 1169145 : ...................ag{AC}CCTGCACCACCCTCCTACGGGACCctgtgcc : 1095595 31 : laSerArgAspAspTrp >>>> Target Intron 2 >>>> ArgCysAlaA : 39 ||| ......||| 34134 bp !:!! !:!!| roSerSerSerSerTrp+- ++LysSerSerA 1095594 : cctcctcctcctcatgggg.........................agAAATCTTCAA : 1061434 40 : rgSerMetHis >>>> Target Intron 3 >>>> GluPheSerAla >> : 47 |||||...!!. 44074 bp |||||||||!.! rgSerGlnGln++ ++GluPheSerGly++ 1061433 : GGAGCCAACAAgt.........................agGAATTCTCCGGGgt.. : 1017334 48 : >> Target Intron 4 >>>> >>>> Target Intron 5 >>>> Ly : 47 40578 bp 132886 bp || ++++ ++Ly 1017333 : .......................aggt.........................agAA : 843872 48 : sAspIleAsp{G} >>>> Target Intron 6 >>>> {ly}HisMetValA : 55 |..!:!: {|} 42718 bp {||}...::: | sSerLeuArg{G}++ ++{ly}ArgLeuPheA 843871 : AAGCTTGAGA{G}gt.........................ag{gc}agactgttta : 801130 56 : sn{L} >>>> Target Intron 7 >>>> {eu}AspLysTyrArgGlyPhe : 62 ||{:} 82858 bp {!!}|||:!! |||..!! sn{V}++ ++{al}AspGluIleArgSerSer 801129 : ac{G}gt.........................ag{TT}GATGAAATAAGGTCCTCA : 718251 63 : ValCysIleValThrAsnValAlaSerGlnUnk >>>> Target Intron 8 : 74 ||| ! ! ! :::::::::...:::||| 194344 bp ValArgSerTrpSerSerLeuValAlaGlnMet++ 718250 : GTGAGAAGCTggtcttccctcgtggctcagatggt..................... : 718213 75 : >>>> >>>> Target Intron 9 >>>> GlyLysThrGluValAsnTyr : 80 12006 bp !||| !:!!:!!||| !! ++++ ++IleLysAspLysLeuAsnAsp 718212 : ....aggt.........................agATCAAAGATAAATTGAATGAT : 511847 81 : ThrGlnLeuValAspLeuHis >>>> Target Intron 10 >>>> AlaAr : 89 !.!|||! !||||||! !.!. 42344 bp ||||| AsnGlnGlnValAspHisGlu++ ++AlaAr 511846 : AACCAACAAGTCGACCACGAGgt..........................agGCTAG : 469476 90 : gTyrAla{Gl} >>>> Target Intron 11 >>>> {u}CysGly{Le} : 95 |!:!:!!{..} 3252 bp {.}||| !{ } gPheSer{Se}++ ++{r}CysPro{Se}++ 469475 : ATTCTCG{AG}gt..........................ag{C}TGCCCC{AG}gt : 466204 96 : >>>> Target Intron 12 >>>> {u}ArgIleLeuAlaPheProCysAsnG : 104 90640 bp {!} !!||||||||||||||| !!...| ++{r}GlyIleLeuAlaPheProGlyArgG 466203 : ..........................ag{C}GGTATTTTGGCGTTCCCAGGCCGGC : 375539 105 : ln{P} >>>> Target Intron 13 >>>> {he}GlyLysGlnGluProGl : 111 ||{ } 15673 bp { !}|||:!! !...||||| ln{G}++ ++{ly}GlyGlnGlySerProGl 375538 : AG{G}gt..........................ag{GC}GGGCAGGGGTCTCCAGG : 359845 112 : ySerAsnGluGluIleLysGlu >>>> Target Intron 14 >>>> PheA : 120 |!!!...|||.!.|||:!!||| 43403 bp .!!| yCysGlyGluHisIleGlnGlu++ ++LeuA 359844 : ATGCGGGGAGCACATACAGGAGgt..........................agCTTG : 316415 121 : la{Al} >>>> Target Intron 15 >>>> {a}GlyTyrAsnValLysPh : 127 ||{ } 61303 bp {!}|||:!!::!||| la{Le}++ ++{u}GlyHisSerValProHi 316414 : CC{CT}gt..........................ag{G}GGGCACTCTGTCCCCCA : 255091 128 : eAspMetPheSerLysIleCysValAsnGlyAspAspAlaHisProLeuTrpLys : 146 !.!.! !!!! ! ! ! |||!..|||:!!! !.!!!.|||! !|||!:! sGlnThrAlaThrLeuHisArgValArgGlyAsnGlyGlyGlnProProTrpArg- 255090 : CCAAACAGCCACCCTGCACAGGGTCAGGGGGAATGGGGGGCAGCCTCCCTGGAGGc : 255034 147 : >>>> Target Intron 16 >>>> TrpMetLysIleGlnProLys >>>> : 153 42829 bp :!! ! :!!!!.|||:!! + -+AsnLeuAspValHisProGln++ 255033 : t..........................tgAATTTGGATGTCCACCCCCAGgt.... : 212182 154 : Target Intron 17 >>>> GlyLysGlyIleLeuGlyAsnAlaIleLys : 163 59416 bp ||| !|||:::||||||......:::::: ++GlyProGlyLeuLeuGlyGlyGlyValGlu++ 212181 : ......................agGGGCCGGgattgttgggggggggggtggaggt : 152738 164 : >>>> Target Intron 18 >>>> TrpAsnPheThr{Ly} >>>> Targe : 167 81523 bp |||||| !|||{::} ++TrpAsnHisThr{Ar}++ 152737 : ..........................agTGGAATCACACC{CG}gt.......... : 71199 168 : t Intron 19 >>>> {s}PheLeuIle{As} >>>> Target Intron 2 : 171 6199 bp {:}|||::: { } 3460 bp ++{g}PheIleGlu{Me}++ 71198 : ................ag{t}tttattgag{at}gt.................... : 64988 172 : 0 >>>> {p}LysAsnGlyCysVal >>>> Target Intron 21 >>>> : 177 { }|||...||| !!||| 19566 bp ++{t}LysGlyGlySerVal++ ++ 64987 : ......ag{G}AAGGGAGGCAGTGTGgt..........................ag : 41950 178 : >>>> Target Intron 22 >>>> ValLysArgTyrGlyProMetGluGl : 185 37213 bp !!..!!!!::||||||! ! || ++ ++ArgSerSerTrpGlyProLysGlyGl 41949 : gt..........................agAGGAGCAGCTGGGGTCCCAAAGGCGA : 4711 186 : uProLeuValIleGlu : 190 | !!!!|||:!::!! uGluPheValLeuGln 4710 : GGAATTTGTCCTGCAG : 4694 vulgar: SPP00000007_1.0 3 190 . NIVO01030317.1:subseq(555646,1182752) 1169194 4693 - 135 M 15 45 S 0 1 5 0 2 I 0 73517 3 0 2 S 1 2 M 16 48 5 0 2 I 0 34130 3 0 2 M 7 21 5 0 2 I 0 44070 3 0 2 M 4 12 5 0 2 I 0 40574 3 0 2 5 0 2 I 0 132882 3 0 2 M 4 12 S 0 1 5 0 2 I 0 42714 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 82854 3 0 2 S 1 2 M 17 51 5 0 2 I 0 194340 3 0 2 5 0 2 I 0 12002 3 0 2 M 14 42 5 0 2 I 0 42340 3 0 2 M 4 12 S 0 2 5 0 2 I 0 3248 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 90636 3 0 2 S 1 1 M 9 27 S 0 1 5 0 2 I 0 15669 3 0 2 S 1 2 M 13 39 5 0 2 I 0 43399 3 0 2 M 2 6 S 0 2 5 0 2 I 0 61299 3 0 2 S 1 1 M 24 72 5 0 2 I 0 42825 3 0 2 M 7 21 5 0 2 I 0 59412 3 0 2 M 10 30 5 0 2 I 0 81519 3 0 2 M 4 12 S 0 2 5 0 2 I 0 6195 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 3456 3 0 2 S 1 1 M 5 15 5 0 2 I 0 19562 3 0 2 5 0 2 I 0 37209 3 0 2 M 14 42 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 4694 1169194 135 - . gene_id 9 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1169149 1169194 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1169149 1169194 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1169147 1169148 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1095628 1169148 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1095628 1095629 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1095578 1095627 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1095578 1095627 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1095576 1095577 . - . intron_id 2 ; splice_site "gG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1061444 1095577 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1061444 1061445 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1061423 1061443 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1061423 1061443 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1061421 1061422 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1017349 1061422 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1017349 1017350 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1017337 1017348 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1017337 1017348 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1017335 1017336 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1017336 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 843873 976758 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 843873 843874 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 843860 843872 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 843860 843872 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 843858 843859 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 801142 843859 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 801142 801143 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 801127 801141 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 801127 801141 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 801125 801126 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 718269 801126 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 718269 718270 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 718216 718268 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 718216 718268 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 718214 718215 . - . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 718215 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 511866 523871 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 511866 511867 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 511824 511865 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 511824 511865 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 511822 511823 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 469480 511823 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 469480 469481 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 469466 469479 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 469466 469479 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 469464 469465 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 466214 469465 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 466214 466215 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 466205 466213 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 466205 466213 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 466203 466204 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 375565 466204 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 375565 375566 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 375536 375564 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 375536 375564 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 375534 375535 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 359863 375535 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 359863 359864 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 359822 359862 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 359822 359862 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 359820 359821 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 316419 359821 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 316419 316420 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 316411 316418 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 316411 316418 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 316409 316410 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 255108 316410 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 255108 255109 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 255035 255107 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 255035 255107 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 255033 255034 . - . intron_id 16 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 212206 255034 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 212206 212207 . - . intron_id 15 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 212185 212205 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 212185 212205 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 212183 212184 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 152769 212184 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 152769 152770 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 152739 152768 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 152739 152768 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 152737 152738 . - . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 71216 152738 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 71216 71217 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 71202 71215 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 71202 71215 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 71200 71201 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 65003 71201 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 65003 65004 . - . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64991 65002 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64991 65002 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64989 64990 . - . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 61531 64990 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 61531 61532 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 61515 61530 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 61515 61530 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 61513 61514 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 61514 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 4736 41948 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 4736 4737 . - . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 4694 4735 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 4694 4735 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 4694 1169194 135 - . alignment_id 9 ; Query SPP00000007_1.0 ; Align 1169195 4 45 ; Align 1095626 20 48 ; Align 1061444 36 21 ; Align 1017349 43 12 ; Align 843873 47 12 ; Align 801140 52 12 ; Align 718267 57 51 ; Align 511866 74 42 ; Align 469480 88 12 ; Align 466213 93 6 ; Align 375564 96 27 ; Align 359861 106 39 ; Align 316419 119 6 ; Align 255107 122 72 ; Align 212206 146 21 ; Align 152769 153 30 ; Align 71216 163 12 ; Align 65002 168 9 ; Align 61530 172 15 ; Align 4736 177 42 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 133 Query range: 1 -> 194 Target range: 1064286 -> 27806 2 : SerLeuGlyArgLeuCysArgLeuLeu >>>> Target Intron 1 >>>> : 11 ||||||||||||||||||!:! !||| 87501 bp SerLeuGlyArgLeuCysLysAspLeu++ ++ 1064286 : TCACTCGGTCGGCTCTGCAAAGACCTGgt.........................ag : 976760 12 : >>>> Target Intron 2 >>>> LysProAlaLeuLeuCysGlyAlaLeu : 19 44919 bp :!!||| !:!!:!!|||! !|||||| ++ ++GluProArgMetMetCysGluAlaLeu 976759 : gt.........................agGAGCCGCGGATGATGTGCGAGGCATTA : 931815 20 : {A} >>>> Target Intron 3 >>>> {la}AlaProGlyLeuAlaGlyTh : 27 { } 140382 bp { !}|||||||||:!!! !||| {T}++ ++{yr}AlaProGlyValGluGlyAs 931814 : {T}gt.........................ag{AT}GCTCCAGGGGTTGAGGGAGA : 791409 28 : rMetCysAlaSerArgAsp{A} >>>> Target Intron 4 >>>> {sp}T : 35 !:!:! ! !!:!!:!!!:{!} 68976 bp { !}| pLeuPheGlnAsnLysGlu{G}++ ++{ly}T 791408 : TTTATTTCAGAACAAGGAG{G}gt.........................ag{gt}t : 722409 36 : rpArgCysAla >>>> Target Intron 5 >>>> >>>> Target In : 39 ||:::|||... 198526 bp 11419 rpGlnCysVal-+ ++++ 722408 : ggcaatgcgtgct.........................aggt.............. : 523869 40 : tron 6 >>>> ArgSerMetHisGluPheSerAlaLysAspIle{A} >>>> : 50 bp ||| !|||.!.||| ! ! ! ..!! !{ } ++ValSerTrpHisHisPheLeuLeuAspSerThr{P}++ 523868 : ...........aggtaagttgGCATCATTTCCTTTTAGACTCCACA{C}gt..... : 512416 51 : Target Intron 7 >>>> {sp}{G} >>>> Target Intron 8 >>>> : 51 16876 bp { !}{|} 39268 bp ++{ro}{G}++ 512415 : ....................ag{CT}{G}gt......................... : 495537 52 : {ly}HisMetValAsnLeuAspLysTyr{A} >>>> Target Intron 9 : 60 {||}||| !..!|||.!.!:! { } 22230 bp ++{ly}HisGlyProArgLeuGlnArgGly{G}++ 495536 : ag{GG}CACGGACCTCGCCTACAAAGAGGA{G}gt..................... : 456242 61 : >>>> {rg}GlyPheValCysIleValThrAsnValAlaSerGlnUnkGly >> : 75 {!!}|||||| ||| |||!.!! ! !:!! ! ! ++{ly}GlyPhePheProIle***ThrThrGlyLeuAlaTyrLeuGln++ 456241 : ....ag{gc}ggattctttcccatctgaaccACTGGCTTAGCCTATCTGCAGgt.. : 433968 76 : >> Target Intron 10 >>>> LysThrGluValAsnTyrThrGlnLeuVal : 84 114795 bp !:!|||! ! ! ||||||! !||| ! ++ArgThrAlaHisAlaTyrThrLeuLeuGln 433967 : ........................agAGAACAGCACACGCATACACCCTACTGCAG : 319148 85 : AspLeuHisAlaArg >>>> Target Intron 11 >>>> TyrAlaGluCy : 93 |||||| ! ! 33694 bp ! ..!||||| ArgLeuHisGlnSer++ ++GluCysGluCy 319147 : AGACTCCACCAGTCCgt..........................agGAGTGCGAGTG : 285427 94 : sGlyLeuArgIleLeuAlaPheProCysAsn >>>> Target Intron 12 > : 104 |||||||! !! !|||||| ||||||.!. 19541 bp sGlyLeuProThrLeuAlaGlnProCysGln+- 285426 : TGGGCTCCCCACTCTAGCTCAGCCATGCCAAga....................... : 285392 105 : >>> GlnPheGlyLysGlnGluProGlySerAsn >>>> Target Intron : 114 :!!||| ! !|||.!.|||||| !!||| 8600 bp -+GluPheGlnGlyGlnHisProGlyProAsn++ 285391 : ...ggGAATTTCAGGGGCAGCATCCTGGGCCAAATgt................... : 265821 115 : 13 >>>> GluGluIleLys >>>> Target Intron 14 >>>> GluPh : 119 !!:! !:!! 44297 bp |||!: ++AspGlyProGln++ ++GluTy 265820 : .......agGATGGCCCACAGgt..........................agGAATA : 212911 120 : eAlaAlaGlyTyrAsnVal >>>> Target Intron 15 >>>> LysPheA : 128 ! !|||! !!::!!.||| 22697 bp |||!::! rHisAlaAspTrpLysVal++ ++LysTrpG 212910 : CCATGCAGACTGGAAAGTGgt..........................agAAATGGG : 190187 129 : spMetPheSerLysIleCysValAsnGlyAspAsp{A} >>>> Target Intr : 140 !: .!!!!!!:!||| !|||::!|||..!! {!} 36356 b luGlyLeuCysArgIleAsnValSerGlySerAla{A}++ 190186 : AAGGCCTTTGCAGAATAAATGTGTCTGGCAGTGCA{G}gt................ : 190148 141 : on 16 >>>> {la}HisProLeuTrpLysTrpMetLysIleGlnProLysGlyL : 154 p { !} |||! ! ! !:!||| !!:!:!:.!. !!..|||| ++{sp}SerProArgGlyArgTrp***ArgLeuAsnGlySerGlyL 190147 : ..........ag{AC}TCACCTCGGGGCAGGTGGTAGAGGCTGAATGGGAGCGGAA : 153753 155 : ysGlyIleLeuGlyAsn >>>> Target Intron 17 >>>> AlaIleLys : 162 ||.!! ||| !!.!. 81305 bp |||:!! ysSerArgLeu***Gln++ ++AlaLeuLeu 153752 : AGAGCCGGCTGTGACAGgt..........................aggcCCTCCTC : 72424 163 : TrpAsnPheThrLysPheLeuIleAspLysAsnGlyCysVal{V} >>>> Targ : 177 |||::!! ! !! !::!!!.!! !.. !|||||| !{!} TrpSerSerProLeuTrpPhePheTrpSerProGlyCysArg{A}++ 72423 : TGGTCCTCCCCACTCTGGTTTTTCTGGAGTCCTGGCTGCAGG{G}gt......... : 72376 178 : et Intron 18 >>>> {al}LysArgTyrGlyProMetGlu{Gl} >>>> T : 185 8038 bp {.!} !.!!:!..! !! {..} ++{la}ProHisPheSerSerAlaThr{Se}++ 72375 : .................ag{CA}CCCCACTTCTCATCAGCTACC{AG}gt...... : 64313 186 : arget Intron 19 >>>> {u}ProLeuValIleGlu >>>> Target In : 191 1932 bp {.}|||||||||:!:||| 20419 ++{r}ProLeuValValGlu-+ 64312 : ....................ag{C}CCCCTAGTAGTGGAGct.............. : 62365 192 : tron 20 >>>> >>>> Target Intron 21 >>>> LysAspLeuPro : 194 bp 14130 bp |||! !|||||| ++++ ++LysAlaLeuPro 62364 : ............aggt..........................agAAAGCCCTCCCA : 27809 vulgar: SPP00000007_1.0 1 194 . NIVO01030317.1:subseq(555646,1182752) 1064286 27806 - 133 M 9 27 5 0 2 I 0 87497 3 0 2 5 0 2 I 0 44915 3 0 2 M 9 27 S 0 1 5 0 2 I 0 140378 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 68972 3 0 2 S 1 2 M 4 12 5 0 2 I 0 198522 3 0 2 5 0 2 I 0 11415 3 0 2 M 11 33 S 0 1 5 0 2 I 0 16872 3 0 2 S 1 3 5 0 2 I 0 39264 3 0 2 S 1 2 M 8 24 S 0 1 5 0 2 I 0 22226 3 0 2 S 1 2 M 14 42 5 0 2 I 0 114791 3 0 2 M 15 45 5 0 2 I 0 33690 3 0 2 M 14 42 5 0 2 I 0 19537 3 0 2 M 10 30 5 0 2 I 0 8596 3 0 2 M 4 12 5 0 2 I 0 44293 3 0 2 M 8 24 5 0 2 I 0 22693 3 0 2 M 14 42 S 0 1 5 0 2 I 0 36352 3 0 2 S 1 2 M 19 57 5 0 2 I 0 81301 3 0 2 M 17 51 S 0 1 5 0 2 I 0 8034 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 1928 3 0 2 S 1 1 M 5 15 5 0 2 I 0 20415 3 0 2 5 0 2 I 0 14126 3 0 2 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 27807 1064286 133 - . gene_id 10 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1064260 1064286 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1064260 1064286 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1064258 1064259 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1064259 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 931840 976758 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 931840 931841 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 931812 931839 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 931812 931839 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 931810 931811 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 791430 931811 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 791430 791431 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 791388 791429 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 791388 791429 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 791386 791387 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 722412 791387 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 722412 722413 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 722398 722411 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 722398 722411 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 722396 722397 . - . intron_id 5 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 722397 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 512453 523871 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 512453 512454 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 512419 512452 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 512419 512452 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 512417 512418 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 495543 512418 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 495543 495544 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 495540 495542 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 495540 495542 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 495538 495539 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 456272 495539 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 456272 456273 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 456245 456271 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 456245 456271 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 456243 456244 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 434015 456244 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 434015 434016 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 433971 434014 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 433971 434014 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 433969 433970 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 319176 433970 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 319176 319177 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 319131 319175 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 319131 319175 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 319129 319130 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 285437 319130 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 285437 285438 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 285395 285436 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 285395 285436 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 285393 285394 . - . intron_id 12 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 265854 285394 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 265854 265855 . - . intron_id 11 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 265824 265853 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 265824 265853 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 265822 265823 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 257224 265823 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 257224 257225 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 257212 257223 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 257212 257223 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 257210 257211 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 212915 257211 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 212915 212916 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 212891 212914 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 212891 212914 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 212889 212890 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 190194 212890 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 190194 190195 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 190151 190193 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 190151 190193 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 190149 190150 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 153795 190150 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 153795 153796 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 153736 153794 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 153736 153794 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 153734 153735 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 72431 153735 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 72431 72432 . - . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 72379 72430 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 72379 72430 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 72377 72378 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64341 72378 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64341 64342 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64316 64340 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64316 64340 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64314 64315 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 62384 64315 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 62384 62385 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 62368 62383 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 62368 62383 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 62366 62367 . - . intron_id 20 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 62367 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 27819 41948 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 27819 27820 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 27807 27818 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 27807 27818 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 27807 1064286 133 - . alignment_id 10 ; Query SPP00000007_1.0 ; Align 1064287 2 27 ; Align 931840 11 27 ; Align 791428 21 39 ; Align 722410 35 12 ; Align 512453 39 33 ; Align 456270 52 24 ; Align 434013 61 42 ; Align 319176 75 45 ; Align 285437 90 42 ; Align 265854 104 30 ; Align 257224 114 12 ; Align 212915 118 24 ; Align 190194 126 42 ; Align 153793 141 57 ; Align 72431 160 51 ; Align 64339 178 21 ; Align 62383 186 15 ; Align 27819 191 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 131 Query range: 0 -> 186 Target range: 1081295 -> 25756 1 : MetSerLeuGlyArgLeuCysArgLeuLeuLys >>>> Target Intron 1 : 12 ||||||||||||:::||| ... ||| 104504 bp MetSerLeuGlyLysLeuArgGluThrLeuMet++ 1081295 : atgagtttgggtaaactccgggagacgctgatggt..................... : 1081260 13 : >>>> >>>> Target Intron 2 >>>> ProAlaLeuLeuCysGly{A} : 18 198911 bp !!||||||||||||!.!{!} ++++ ++AlaAlaLeuLeuCysAla{G} 1081259 : ....aggt.........................agGCAGCCCTCCTGTGTGCA{G} : 777829 19 : >>>> Target Intron 3 >>>> {la}Leu{A} >>>> Target Int : 20 8122 bp {.!} {|} 20483 +- ++{ly}Arg{A}++ 777828 : gg.........................ag{gg}agg{g}gt............... : 769698 21 : ron 4 >>>> {la}AlaPro{G} >>>> Target Intron 5 >>>> {l : 23 bp {||} |||{|} 77190 bp {| ++{la}ProPro{G}++ ++{l 769697 : ..........ag{ca}ccacct{g}gt.........................ag{g : 672018 24 : y}Leu >>>> Target Intron 6 >>>> AlaGlyThrMetCysAlaSerA : 32 |} ! 55721 bp ||||||:!! ! !|||.!!| y}Lys++ ++AlaGlySerAlaSerAlaGlyA 672017 : a}AAGgt.........................agGCTGGGTCAGCTTCTGCAGGAC : 616271 33 : rgAspAspTrpArgCysAlaArgSerMetHisGluPhe{Se} >>>> Target : 45 ||...! ||| !||||||||| !|||! .!!{!!} 304 rgSerGlyAsnArgAspAlaArgSerSerHisAlaLeu{Cy}-+ 616270 : GGTCAGGAAACCGGGATGCGCGTTCGTCGCATGCCCTC{TG}ct............ : 616228 46 : Intron 7 >>>> {r}AlaLysAspIleAspGlyHisMetValAsnLeuAspLy : 58 97 bp {!}::!::!||||||.!.!.! |||::::::...:: ++{s}SerArgAspIleGlnAlaThrGluValSerIleSerAr 616227 : .............ag{T}AGCCGAGACATCCAAGCAACcgaagtgtccatcagcag : 585696 59 : sTyrArgGly{Ph} >>>> Target Intron 8 >>>> {e}ValCys{Il} : 65 : !|||{ } 39634 bp { } !|||{! } gGluMetGly{Ly}++ ++{s}SerCys{Ar} 585695 : agaaatGGGT{AA}gt.........................ag{G}AGCTGC{AG} : 546041 66 : >>>> Target Intron 9 >>>> {e}ValThrAsn{Va} >>>> Targ : 69 68916 bp {!}:!!..!|||{ } ++ ++{g}LeuValAsn{Ly}++ 546040 : gt.........................ag{A}CTGGTGAAC{AA}gt......... : 477109 70 : et Intron 10 >>>> {l}AlaSerGlnUnkGlyLysThrGlu >>>> Tar : 78 7012 bp {!}|||!!!||| !.!!:!||| ++{s}AlaThrGlnGlyAlaArgThrPro++ 477108 : .................ag{A}GCCACGCAGGGAGCCAGGACTCCCgt........ : 470072 79 : get Intron 11 >>>> ValAsnTyrThr >>>> Target Intron 12 : 82 98259 bp |||:::!:!..! 22166 bp ++ValSerPheVal++ 470071 : ..................agGTGTCATTTGTGgt...................... : 371801 83 : >>>> GlnLeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArg{I} : 97 ! ||| !||||||||| ! !! :!! ||| ! ! !!{!} ++ProLeuSerAspLeuHisLeuSerLeuSerThrCysHisLysGly{T}++ 371800 : ....agCCTCTGTCTGATCTTCACCTCTCATTGTCTACCTGTCACAAAGGA{A}gt : 349591 98 : >>>> Target Intron 13 >>>> {le}LeuAlaPhePro{C} >>>> Ta : 102 6004 bp { }! ! !!||||||{|} ++{hr}CysProPhePro{C}++ 349590 : ..........................ag{CG}TGTCCCTTCCCC{T}gt....... : 343570 103 : rget Intron 14 >>>> {ys}AsnGlnPheGlyLysGlnGluPro{Gl} > : 111 4543 bp {||}||| !.!! !:!!::!:!!|||{||} ++{ys}AsnTrpLeuProGlnArgGlnPro{Gl}-+ 343569 : ...................ag{GC}AATTGGCTTCCTCAGAGGCAACCA{GG}ct. : 338999 112 : >>> Target Intron 15 >>>> {y}SerAsnGluGluIleLysGlu{P} : 119 59115 bp {|}:::|||...||| !||| !{ } ++{y}AlaAsnSerGluSerLysThr{G}++ 338998 : .........................ag{g}gcgAACAGTGAATCAAAAACG{G}gt : 279863 120 : >>>> Target Intron 16 >>>> {he}AlaAlaGlyTyrAsnValLysPhe : 127 65182 bp { !} !::!..! !!:!!..!...||| ++{ly}LeuSerSerAspAspThrSerPhe 279862 : ..........................ag{GC}CTCAGCTCAGATGACACCTCCTTT : 214658 128 : {A} >>>> Target Intron 17 >>>> {sp}MetPheSerLysIleCysV : 135 {|} 19194 bp {||}:!: !!!:!! !||| {A}+- ++{sp}LeuLysThrGlnCysCys* 214657 : {G}gg..........................ag{AC}CTCAAGACCCAATGTTGTT : 195440 136 : alAsnGlyAspAspAlaHisProLeuTrpLysTrpMetLysIleGln >>>> Ta : 151 !!.. ! !...!.!|||||| |||:::|||::: :::||| **ThrProPheSerGlyHisPro---TrpGlnTrpLeuGlyLeuGln++ 195439 : AAACACCTTTTTCAGGACATCCC---tggcagtggttaggactccaggt....... : 195393 152 : rget Intron 18 >>>> ProLysGlyLysGlyIleLeuGlyAsnAla >>> : 161 58921 bp ||| !||||||!.!:!! !|||..!:!! ++ProProGlyLysAlaLeuThrGlyArgSer++ 195392 : ...................agCCCCCGGGGAAGGCCCTCACCGGCCGCTCTgt... : 136442 162 : > Target Intron 19 >>>> IleLys >>>> Target Intron 20 > : 163 78198 bp :!:||| 5803 bp ++ValLys++ 136441 : .......................agGTGAAAgt....................... : 58238 164 : >>> TrpAsnPheThrLys >>>> Target Intron 21 >>>> >>>> : 168 |||..! ! !:!! 10474 bp ++TrpGlyValGlyGlu++ ++++ 58237 : ...agTGGGGTGTGGGTGAGgt..........................aggt.... : 41946 169 : Target Intron 22 >>>> PheLeuIleAspLysAsnGlyCys<->ValVa : 177 16132 bp ||| ... ...|||||| !|| ++AlaLeuProSerAlaGlnGlyCysProLysVa 41945 : ......................aggcccttccctctgcccaggGCTGCCCCAAGGT : 25786 178 : lLysArgTyrGlyProMetGluGluPro : 186 |:!!||| ||||||:!! !||| lGlnArgArgGlyProLeuProTrpPro 25785 : GCAAAGAAGGGGGCCCCTGCCCTGGCCA : 25757 vulgar: SPP00000007_1.0 0 186 . NIVO01030317.1:subseq(555646,1182752) 1081295 25756 - 131 M 11 33 5 0 2 I 0 104500 3 0 2 5 0 2 I 0 198907 3 0 2 M 6 18 S 0 1 5 0 2 I 0 8118 3 0 2 S 1 2 M 1 3 S 0 1 5 0 2 I 0 20479 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 77186 3 0 2 S 1 2 M 1 3 5 0 2 I 0 55717 3 0 2 M 20 60 S 0 2 5 0 2 I 0 30493 3 0 2 S 1 1 M 16 48 S 0 2 5 0 2 I 0 39630 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 68912 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 7008 3 0 2 S 1 1 M 8 24 5 0 2 I 0 98255 3 0 2 M 4 12 5 0 2 I 0 22162 3 0 2 M 15 45 S 0 1 5 0 2 I 0 6000 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 4539 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 59111 3 0 2 S 1 1 M 7 21 S 0 1 5 0 2 I 0 65178 3 0 2 S 1 2 M 8 24 S 0 1 5 0 2 I 0 19190 3 0 2 S 1 2 M 14 42 G 1 0 M 7 21 5 0 2 I 0 58917 3 0 2 M 10 30 5 0 2 I 0 78194 3 0 2 M 2 6 5 0 2 I 0 5799 3 0 2 M 5 15 5 0 2 I 0 10470 3 0 2 5 0 2 I 0 16128 3 0 2 M 8 24 G 0 3 M 11 33 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 25757 1081295 131 - . gene_id 11 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1081263 1081295 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1081263 1081295 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1081261 1081262 . - . intron_id 1 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1081262 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 777848 976758 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 777848 777849 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 777829 777847 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 777829 777847 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 777827 777828 . - . intron_id 3 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 769707 777828 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 769707 769708 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 769701 769706 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 769701 769706 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 769699 769700 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 749218 769700 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 749218 749219 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 749209 749217 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 749209 749217 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 749207 749208 . - . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 672019 749208 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 672019 672020 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 672014 672018 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 672014 672018 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 672012 672013 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 616293 672013 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 616293 616294 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 616231 616292 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 616231 616292 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 616229 616230 . - . intron_id 7 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 585734 616230 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 585734 585735 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 585683 585733 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 585683 585733 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 585681 585682 . - . intron_id 8 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 585682 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 546039 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 470100 477111 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 470100 470101 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 470075 470099 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 470075 470099 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 470073 470074 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 371816 470074 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 371816 371817 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 371804 371815 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 371804 371815 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 371802 371803 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 349638 371803 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 349638 349639 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 349592 349637 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 349592 349637 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 349590 349591 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 343588 349591 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 343588 343589 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 343573 343587 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 343573 343587 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 343571 343572 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 339030 343572 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 339030 339031 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 339002 339029 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 339002 339029 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 339000 339001 . - . intron_id 15 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 279887 339001 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 279887 279888 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 279864 279886 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 279864 279886 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 279862 279863 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 214682 279863 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 214682 214683 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 214655 214681 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 214655 214681 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 214653 214654 . - . intron_id 17 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 195461 214654 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 195461 195462 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 195396 195460 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 195396 195460 . - . insertions 0 ; deletions 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 195394 195395 . - . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 136475 195395 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 136475 136476 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 136445 136474 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 136445 136474 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 136443 136444 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 58247 136444 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 58247 58248 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 58241 58246 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 58241 58246 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 58239 58240 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 52438 58240 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 52438 52439 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 52423 52437 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 52423 52437 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 52421 52422 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 52422 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 25817 41948 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 25817 25818 . - . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 25757 25816 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 25757 25816 . - . insertions 3 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 25757 1081295 131 - . alignment_id 11 ; Query SPP00000007_1.0 ; Align 1081296 1 33 ; Align 777848 12 18 ; Align 769705 19 3 ; Align 749216 21 6 ; Align 672017 24 3 ; Align 616293 25 60 ; Align 585733 46 48 ; Align 546048 63 6 ; Align 477123 66 9 ; Align 470099 70 24 ; Align 371816 78 12 ; Align 349638 82 45 ; Align 343586 98 12 ; Align 339028 103 24 ; Align 279886 112 21 ; Align 214680 120 24 ; Align 195459 129 42 ; Align 195417 144 21 ; Align 136475 151 30 ; Align 58247 161 6 ; Align 52438 163 15 ; Align 25817 168 24 ; Align 25790 176 33 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 130 Query range: 24 -> 172 Target range: 1160558 -> 618 25 : AlaGlyThrMetCysAlaSerArgAspAspTrp{Ar} >>>> Target Intro : 36 :!!..!||| !|||.!! !!||| !...|||{||} 33243 bp SerSerThrTrpCysThrArgArgProSerTrp{Ar}++ 1160558 : TCGTCCACATGGTGTACACGACGGCCCTCGTGG{AG}gt................. : 1160521 37 : n 1 >>>> {g}CysAlaArgSer{Me} >>>> Target Intron 2 >>>> : 41 {|}|||!.! ! !!{! } 23780 bp ++{g}CysGlySerPro{Ar}++ 1160520 : ........ag{G}TGTGGTTCCCCC{AG}gt......................... : 1127263 42 : {t}HisGluPheSerAlaLysAspIleAsp{Gl} >>>> Target Intron : 51 {!}|||!!:||||||! !:::|||:!!..!{ !} 42012 bp -+{g}HisAspPheSerGluArgAspLeuSer{Ar}++ 1127262 : cg{G}CACGACTTCTCCGAGCGCGACCTCAGC{CG}gt.................. : 1103453 52 : 3 >>>> {y}HisMetValAsnLeuAsp{Ly} >>>> Target Intron 4 : 58 {!}:!!:!: !... !:!!{||} 84664 bp ++{g}AsnLeuGlnGlyAlaAsn{Ly}++ 1103452 : .......ag{A}AATCTTCAAGGAGCCAAC{AA}gt.................... : 1061420 59 : >>>> {s}Tyr >>>> Target Intron 5 >>>> {A} >>>> Targ : 60 {|}||| 18291 bp {.} 1 ++{s}Tyr++ ++{G}++ 1061419 : .....ag{G}TATgt.........................ag{G}gt......... : 958460 61 : et Intron 6 >>>> {rg}GlyPheValCysIleValThrAsnValAlaSer{ : 72 41422 bp {.!}|||||| ! !:!!||||||!.!||| !:!!{ ++{lu}GlyPheTyrValLeuValThrThrValGlnAla{ 958459 : ................ag{AG}GGATTTTATGTCCTTGTTACAACTGTCCAGGCT{ : 817005 73 : Gl} >>>> Target Intron 7 >>>> {n}Unk{Gl} >>>> Target : 74 ||} 47297 bp {|} {||} 124 Gl}++ ++{n}Glu{Gl}++ 817004 : CA}gt.........................ag{g}gag{gg}gt............ : 769698 75 : Intron 8 >>>> {y}LysThrGluValAsnTyrThr{Gl} >>>> Target : 82 00 bp {|}|||.!!|||!.!.!.!:!:!!{ } 55 ++{y}LysAlaGluAlaGlnPheSer{Gl}++ 769697 : .............ag{G}AAAGCAGAAGCGCAGTTTTCA{GG}gt........... : 757274 83 : Intron 9 >>>> {n}LeuValAspLeuHisAlaArgTyrAla >>>> Tar : 92 78 bp {!} ::: ||| ::: ++{y}GlnIleProTyrHisLeuSerHisGln++ 757273 : ..............ag{g}cagattccttaccatctgagccaccaggt........ : 751668 93 : get Intron 10 >>>> GluCys{Gl} >>>> Target Intron 11 >> : 94 22034 bp |||{ !} 43739 bp ++LeuCys{Ar}++ 751667 : ..................agCTTTGC{AG}gt........................ : 729626 95 : >> {y}LeuArgIleLeuAla >>>> Target Intron 12 >>>> PheP : 101 {!}|||!:!|||! ! 50407 bp |||| ++{g}LeuLysIleTyrGln++ ++PheP 729625 : ..ag{A}CTGAAGATCTACCaggt..........................agtttc : 635463 102 : roCysAsnGlnPhe{G} >>>> Target Intron 13 >>>> {ly}LysGl : 108 || ! |||:!! {|} 34867 bp {||}|||:! roGlyAsnLysGln{G}++ ++{ly}LysGl 635462 : ctgGGAATAAACAA{G}gt..........................ag{GA}AAGGA : 600575 109 : n >>>> Target Intron 14 >>>> {Gl} >>>> Target Intron : 109 ! 22844 bp {..} 29338 bp u++ ++{Se}++ 600574 : Agt..........................ag{AG}gt................... : 577724 110 : 15 >>>> {u}ProGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyrAs : 124 {.}||||||.!!...|||! !:!!...||| !|||||| ! .! ++{r}ProGlyGlyArgGluGlyValSerGluThrAlaAlaThrIleGl 577723 : .......ag{C}CCTGGGGGCCGGGAGGGGGTTTCCGAAACTGCAGCAACAATAGA : 548345 125 : n >>>> Target Intron 16 >>>> >>>> Target Intron 17 > : 125 . 24471 bp 22947 bp u-+ ++++ 548344 : Gat..........................aggt....................... : 523869 126 : >>> ValLysPheAspMetPheSerLysIle{Cy} >>>> Target Intron : 134 |||||| ! :!:||||||||| { !} 51824 bp -+ValLysGlyAlaValPheSerLysGly{Se}++ 523868 : ...tgGTGAAAGGAGCAGTTTTCTCAAAGGGG{AG}gt.................. : 500893 135 : 18 >>>> {s}ValAsn{G} >>>> Target Intron 19 >>>> {ly} : 137 {!}:!!!..{|} 7556 bp {||} ++{r}IleArg{G}++ ++{ly} 500892 : ........ag{T}ATTAGG{G}gt..........................ag{gg} : 441507 138 : AspAspAlaHisPro >>>> Target Intron 20 >>>> LeuTrpLys : 146 ||| ||||||||| 40675 bp !|||!:! AspLeuAlaHisPro+- ++AlaTrpArg++ 441506 : gatcttgcccacccagg..........................agGCCTGGAGGgt : 400806 147 : >>>> Target Intron 21 >>>> TrpMetLysIleGlnProLys >>>> : 153 98434 bp |||! !..:!!!!.|||||| ++TrpAsnSerValHisProLys++ 400805 : ..........................agTGGAACAGTGTCCATCCAAAGgt..... : 302349 154 : Target Intron 22 >>>> GlyLysGlyIle{L} >>>> Target Intr : 157 157937 bp ||| |||:::{:} 35778 b ++GlyValGlyVal{V}++ 302348 : .....................agggggtgggggtg{g}gt................ : 144399 158 : on 23 >>>> {eu}GlyAsnAlaIleLys{Tr} >>>> Target Intron : 163 p {!!}|||!..:!!:!!|||{||} 107958 bp ++{al}GlyArgSerLeuLys{Tr}-+ 144398 : ..........ag{TT}GGTAGGTCTCTTAAA{TG}tt................... : 108602 164 : 24 >>>> {p}AsnPheThrLysPheLeuIleAspLys : 172 {|}..!|||:!!:!!|||:!!||| !||| ++{p}GlyPheSerGlnPheIleIleCysLys 108601 : .......ag{g}GGCTTCTCCCAATTTATAATTTGTAAA : 619 vulgar: SPP00000007_1.0 24 172 . NIVO01030317.1:subseq(555646,1182752) 1160558 618 - 130 M 11 33 S 0 2 5 0 2 I 0 33239 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 23776 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 42008 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 84660 3 0 2 S 1 1 M 1 3 5 0 2 I 0 18287 3 0 2 S 0 1 5 0 2 I 0 141418 3 0 2 S 1 2 M 11 33 S 0 2 5 0 2 I 0 47293 3 0 2 S 1 1 M 1 3 S 0 2 5 0 2 I 0 12396 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 5574 3 0 2 S 1 1 M 9 27 5 0 2 I 0 22030 3 0 2 M 2 6 S 0 2 5 0 2 I 0 43735 3 0 2 S 1 1 M 5 15 5 0 2 I 0 50403 3 0 2 M 6 18 S 0 1 5 0 2 I 0 34863 3 0 2 S 1 2 M 2 6 5 0 2 I 0 22840 3 0 2 S 0 2 5 0 2 I 0 29334 3 0 2 S 1 1 M 15 45 5 0 2 I 0 24467 3 0 2 5 0 2 I 0 22943 3 0 2 M 9 27 S 0 2 5 0 2 I 0 51820 3 0 2 S 1 1 M 2 6 S 0 1 5 0 2 I 0 7552 3 0 2 S 1 2 M 5 15 5 0 2 I 0 40671 3 0 2 M 3 9 5 0 2 I 0 98430 3 0 2 M 7 21 5 0 2 I 0 157933 3 0 2 M 4 12 S 0 1 5 0 2 I 0 35774 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 107954 3 0 2 S 1 1 M 9 27 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 619 1160558 130 - . gene_id 12 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1160524 1160558 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1160524 1160558 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1160522 1160523 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1127281 1160523 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1127281 1127282 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1127266 1127280 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1127266 1127280 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1127264 1127265 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1103486 1127265 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1103486 1103487 . - . intron_id 1 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1103456 1103485 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1103456 1103485 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1103454 1103455 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1061444 1103455 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1061444 1061445 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1061423 1061443 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1061423 1061443 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1061421 1061422 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1061422 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 976755 976758 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 976755 976758 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976753 976754 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 958464 976754 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 958464 958465 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 958463 958463 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 958463 958463 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 958461 958462 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 817041 958462 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 817041 817042 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 817004 817040 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 817004 817040 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 817002 817003 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 769707 817003 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 769707 769708 . - . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 769701 769706 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 769701 769706 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 769699 769700 . - . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 757301 769700 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 757301 757302 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 757277 757300 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 757277 757300 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 757275 757276 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 751699 757276 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 751699 751700 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 751671 751698 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 751671 751698 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 751669 751670 . - . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 729637 751670 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 729637 729638 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 729629 729636 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 729629 729636 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 729627 729628 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 685890 729628 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 685890 685891 . - . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 685874 685889 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 685874 685889 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 685872 685873 . - . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 635467 685873 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 635467 635468 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 635448 635466 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 635448 635466 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 635446 635447 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 600581 635447 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 600581 600582 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 600573 600580 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 600573 600580 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 600571 600572 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 577729 600572 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 577729 577730 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 577727 577728 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 577727 577728 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 577725 577726 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 548389 577726 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 548389 548390 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 548343 548388 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 548343 548388 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 548341 548342 . - . intron_id 16 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 548342 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 500925 523871 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 500925 500926 . - . intron_id 16 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 500896 500924 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 500896 500924 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 500894 500895 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 449072 500895 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 449072 449073 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 449064 449071 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 449064 449071 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 449062 449063 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 441508 449063 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 441508 441509 . - . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 441491 441507 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 441491 441507 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 441489 441490 . - . intron_id 20 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 400816 441490 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 400816 400817 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 400807 400815 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 400807 400815 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 400805 400806 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 302373 400806 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 302373 302374 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 302352 302372 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 302352 302372 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 302350 302351 . - . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 144415 302351 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 144415 144416 . - . intron_id 21 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 144402 144414 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 144402 144414 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 144400 144401 . - . intron_id 23 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 108624 144401 . - . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 108624 108625 . - . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 108605 108623 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 108605 108623 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 108603 108604 . - . intron_id 24 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 647 108604 . - . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 647 648 . - . intron_id 23 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 619 646 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 619 646 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 619 1160558 130 - . alignment_id 12 ; Query SPP00000007_1.0 ; Align 1160559 25 33 ; Align 1127280 37 12 ; Align 1103485 42 27 ; Align 1061443 52 18 ; Align 976758 59 3 ; Align 817039 61 33 ; Align 769706 73 3 ; Align 757300 75 21 ; Align 751698 83 27 ; Align 729637 92 6 ; Align 685889 95 15 ; Align 635467 100 18 ; Align 600579 107 6 ; Align 548388 110 45 ; Align 500925 125 27 ; Align 449071 135 6 ; Align 441506 138 15 ; Align 400816 143 9 ; Align 302373 146 21 ; Align 144415 153 12 ; Align 108622 158 15 ; Align 646 164 27 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 129 Query range: 3 -> 195 Target range: 1152507 -> 239107 4 : GlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuAlaAlaPr : 22 |||! !|||||| !! !||| !|||..!! !! ! ! ||| !|||! !!.!|| GlyProLeuCysCysGlyLeuProProCysSerProGlyGlyArgLeuGluGlyPr 1152507 : GGTCCACTTTGTTGTGGACTTCCACCATGCTCTCCAGGAGGGCGTCTGGAAGGCCC : 1152453 23 : oGlyLeuAlaGly >>>> Target Intron 1 >>>> ThrMetCysAlaSe : 31 ||||||| !!||| 77378 bp !!!: !|||! oGlyLeuProGly-+ ++GlyIleAsnAlaPh 1152452 : AGGCCTCCCAGGGct.........................agGGAATTAATGCCTT : 1075048 32 : rArgAspAspTrpArgCysAlaArg{S} >>>> Target Intron 2 >>>> : 40 !|||! !... !|||..!||| !{.} 4545 bp eArgGlySerGlnArgAlaAlaAla{G}++ + 1075047 : TAGGGGCTCACAGCGGGCTGCGGCT{G}gt.........................a : 1070477 41 : {er}MetHisGluPheSerAlaLysAspIleAspGlyHisMetValAsnLeuAsp : 57 {!!} ...... ... ......:!!||||||.!.|||.!. +{ly}SerGlnArgValGlyGlnAsp***SerSerSerTyrMetValGluLeuGln 1070476 : g{gg}tcacaaagagtcggacaggactgaagcagctcaTACATGGTTGAGTTACAA : 1070425 58 : LysTyrArgGlyPheValCysIle{Va} >>>> Target Intron 3 >>>> : 66 ! |||! |||||| ||||||{..} 8953 bp AspTyrThrGlyPheArgCysIle{Th}++ + 1070424 : GATTATActggtttcaggtgtata{ac}gt.........................a : 1061445 67 : {l}ThrAsnValAlaSerGln{Un} >>>> Target Intron 4 >>>> { : 73 {!}!.! ! !!.!:!!.!.{ } 150061 bp { +{r}AsnLeuGlnGlyAlaAsn{As}++ ++{ 1061444 : g{A}AATCTTCAAGGAGCCAAC{AA}gt.........................ag{ : 911361 74 : k}GlyLysThrGluValAsnTyrThrGlnLeuVal{A} >>>> Target Intr : 85 } !::!.!!|||!.!.!.!:!:!!||| !|||{!} 99782 b n}LeuArgAlaGluAlaGluPheSerGlnAlaVal{G}++ 911360 : c}ctTCGGGCCGAAGCAGAGTTCTCGCAGGCGGTG{G}gt................ : 911324 86 : on 5 >>>> {sp}LeuHisAlaArgTyrAlaGluCysGlyLeuArgIleLeuAl : 99 p { !} !|||.!!!:!|||::!! ! !!! !|||! !:!::!!|| ++{ly}AlaHisThrGlnTyrSerAlaSerValLeuLeuValValAl 911323 : .........ag{GT}GCACACACGCAGTACAGCGCAAGCGTTCTGCTTGTGGTAGC : 811503 100 : aPheProCysAsnGlnPheGlyLysGln >>>> Target Intron 6 >>>> : 109 | !||||||!.!! !|||! ! ! :!! 1445 bp aAlaProCysThrLeuPheGluTyrGlu++ + 811502 : GGCCCCATGCACTCTGTTTGAATATGAAgt.........................a : 810030 110 : GluPro{G} >>>> Target Intron 7 >>>> {ly}Ser{A} >>>> : 113 ||||||{|} 7283 bp {||}:!!{.} +GluPro{G}++ ++{ly}Ala{A}++ 810029 : ggagcct{g}gt.........................ag{GG}GCC{C}gt..... : 802730 114 : Target Intron 8 >>>> {sn}GluGluIleLysGluPheAlaAlaGlyTyr : 123 18400 bp {.!}||||||:!!|||||| !.!!!.!|||!:! ++{rg}GluGluValLysGluGlyThrGlyGlyPhe 802729 : ....................ag{GT}GAGGAGGTAAAGGAGGGTACAGGCGGTTTC : 784303 124 : AsnVal{Ly} >>>> Target Intron 9 >>>> {s}PheAspMetPheSe : 131 !.. !{!:} 36060 bp {!}! !|||... .. Arg***{Ar}++ ++{g}CysAspPheAspGl 784302 : AGGTAG{AG}gt.........................ag{G}TGTGattttgatgg : 748219 132 : rLysIleCysValAsnGlyAspAspAlaHis >>>> Target Intron 10 > : 142 .|||::: !..!!.! !..!|||:!!||| 44180 bp yLysValThrThrThrThrSerAspSerHis++ 748218 : caaagtaacCACCACCACTTCTGATTCTCACgt....................... : 748184 143 : >>> ProLeuTrpLysTrpMetLysIleGlnPro >>>> Target Intron : 152 ||| |||:::|||...|||::: 31954 bp ++SerLeuLeuLysPheMetSerIleGluSer++ 748183 : ...agagtttgctcaaattcatgtccatcgagtcggt................... : 703974 153 : 11 >>>> LysGlyLys >>>> Target Intron 12 >>>> GlyIleLe : 157 ||||||||| 86103 bp ||| :: -+LysGlyLys++ ++GlySerIl 703973 : .......tgaaaggaAAGgt..........................agggatcgat : 585904 158 : uGlyAsnAlaIleLysTrpAsn >>>> Target Intron 13 >>>> >> : 165 : ... ::: |||... 62009 bp eProGlyLeuLeuHisTrpGln++ ++++ 585903 : cccaggtctcctgcattggcaggt..........................aggt.. : 523869 166 : >> Target Intron 14 >>>> PheThrLysPheLeu{Il} >>>> Targ : 170 6915 bp !|||:!!!::|||{||} ++SerThrGluTrpLeu{Il}++ 523868 : ........................agaGCACAGAGTGGCTC{At}gt......... : 516937 171 : et Intron 15 >>>> {e}AspLys >>>> Target Intron 16 >>>> : 173 686 bp {|}|||||| 39123 bp ++{e}AspLys+- 516936 : .................ag{A}GACAAGgg.......................... : 516244 174 : AsnGlyCysVal >>>> Target Intron 17 >>>> ValLysArgTyr : 180 !.!||| ! 72691 bp :!! ||| !! ++ThrGlyGluGln++ ++IleCysArgAsn 516243 : agACTGGTGAACAAgt..........................agATCTGTCGTAAC : 404411 181 : GlyPro >>>> Target Intron 18 >>>> MetGluGluProLeuValIl : 189 !||| 157358 bp :!: ! ! !! !:!!|| LeuPro++ ++ValThrValThrThrLeuIl 404410 : CTCCCGgt..........................agGTTACTGTGACCACACTGAT : 247026 190 : eGluLysAsp >>>> Target Intron 19 >>>> LeuProHis : 195 |...|||!!: 7898 bp ||||||||| eSerLysGlu++ ++LeuProHis 247025 : AAGCAAAGAGgt..........................agCTGCCCCAT : 239108 vulgar: SPP00000007_1.0 3 195 . NIVO01030317.1:subseq(555646,1182752) 1152507 239107 - 129 M 23 69 5 0 2 I 0 77374 3 0 2 M 13 39 S 0 1 5 0 2 I 0 4541 3 0 2 S 1 2 M 25 75 S 0 2 5 0 2 I 0 8949 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 150057 3 0 2 S 1 1 M 11 33 S 0 1 5 0 2 I 0 99778 3 0 2 S 1 2 M 23 69 5 0 2 I 0 1441 3 0 2 M 2 6 S 0 1 5 0 2 I 0 7279 3 0 2 S 1 2 M 1 3 S 0 1 5 0 2 I 0 18396 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 36056 3 0 2 S 1 1 M 15 45 5 0 2 I 0 44176 3 0 2 M 10 30 5 0 2 I 0 31950 3 0 2 M 3 9 5 0 2 I 0 86099 3 0 2 M 10 30 5 0 2 I 0 62005 3 0 2 5 0 2 I 0 6911 3 0 2 M 5 15 S 0 2 5 0 2 I 0 682 3 0 2 S 1 1 M 2 6 5 0 2 I 0 39119 3 0 2 M 4 12 5 0 2 I 0 72687 3 0 2 M 6 18 5 0 2 I 0 157354 3 0 2 M 10 30 5 0 2 I 0 7894 3 0 2 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 239108 1152507 129 - . gene_id 13 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1152439 1152507 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1152439 1152507 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1152437 1152438 . - . intron_id 1 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1075061 1152438 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1075061 1075062 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1075021 1075060 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1075021 1075060 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1075019 1075020 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1070476 1075020 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1070476 1070477 . - . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1070397 1070475 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1070397 1070475 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1070395 1070396 . - . intron_id 3 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1061444 1070396 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1061444 1061445 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1061423 1061443 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1061423 1061443 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1061421 1061422 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 911362 1061422 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 911362 911363 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 911327 911361 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 911327 911361 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 911325 911326 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 811545 911326 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 811545 811546 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 811474 811544 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 811474 811544 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 811472 811473 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 810029 811473 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 810029 810030 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 810022 810028 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 810022 810028 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 810020 810021 . - . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 802739 810021 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 802739 802740 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 802733 802738 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 802733 802738 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 802731 802732 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 784333 802732 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 784333 784334 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 784293 784332 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 784293 784332 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 784291 784292 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 748233 784292 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 748233 748234 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 748187 748232 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 748187 748232 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 748185 748186 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 704007 748186 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 704007 704008 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 703977 704006 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 703977 704006 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 703975 703976 . - . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 672023 703976 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 672023 672024 . - . intron_id 10 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 672014 672022 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 672014 672022 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 672012 672013 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 585911 672013 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 585911 585912 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 585881 585910 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 585881 585910 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 585879 585880 . - . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 585880 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 516957 523871 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 516957 516958 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 516940 516956 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 516940 516956 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 516938 516939 . - . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 516254 516939 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 516254 516255 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 516247 516253 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 516247 516253 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 516245 516246 . - . intron_id 16 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 516246 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 404421 477111 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 404421 404422 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 404403 404420 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 404403 404420 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 404401 404402 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247045 404402 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247045 247046 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247015 247044 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247015 247044 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247013 247014 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 239117 247014 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 239117 239118 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 239108 239116 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 239108 239116 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 239108 1152507 129 - . alignment_id 13 ; Query SPP00000007_1.0 ; Align 1152508 4 69 ; Align 1075061 27 39 ; Align 1070474 41 75 ; Align 1061443 67 18 ; Align 911361 74 33 ; Align 811543 86 69 ; Align 810029 109 6 ; Align 802737 112 3 ; Align 784331 114 36 ; Align 748232 127 45 ; Align 704007 142 30 ; Align 672023 152 9 ; Align 585911 155 30 ; Align 516957 165 15 ; Align 516253 171 6 ; Align 477124 173 12 ; Align 404421 177 18 ; Align 247045 183 30 ; Align 239117 193 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 127 Query range: 10 -> 196 Target range: 1130465 -> 393882 11 : LysProAlaLeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAlaGlyThr{M} : 28 ||||||.!!:!!||| ! ! !!|||||| !||||||:!! ! !|||{ } LysProThrValLeuArgLysProLeuAlaLysProGlyIleHisHisThr{G}++ 1130465 : AAACCTACAGTTCTAAGGAAACCGTTGGCAAAACCAGGCATCCACCACACA{G}gt : 1130413 29 : >>>> Target Intron 1 >>>> {et}CysAlaSerArgAspAsp >>>> : 35 809 bp { !} !!!!||| ! ++{ly}ThrGlnArgArgProGly++ 1130412 : .........................ag{GG}ACACAGAGGAGACCGGGGgt..... : 1129582 36 : Target Intron 2 >>>> TrpArgCysAlaArgSerMet >>>> Target : 42 28794 bp !!||||||:!!|||:!! ! 43 ++GlyArgCysSerArgAlaGlu++ 1129581 : ....................agGGGCGCTGTTCCCGGGCGGAGgt........... : 1100767 43 : Intron 3 >>>> HisGluPheSerAlaLysAspIleAspGlyHisMetValA : 55 213 bp ... ...::: ::: |||::::::: ++GlyThrLeuProThrArgGlySerAsnProHisLeuLeuH 1100766 : ..............aggggaccctccccacccggggatccaacccacacctcctgc : 1057517 56 : snLeuAspLysTyr{Ar} >>>> Target Intron 4 >>>> {g}GlyPhe : 62 ::||| {||} 6897 bp {|}...... isLeuLeuAspCys{Ar}++ +-{g}SerIle 1057516 : acctcctggactgc{ag}gt.........................at{g}tccatt : 1050599 63 : ValCysIleValThrAsnValAlaSerGlnUnkGlyLys >>>> Target Int : 76 ||||||::: |||:::||| ::::::...... ! 73799 ValCysValSerThrAspValHisThrArgThrSerHis++ 1050598 : gtgtgtgtatctacagatgtacacacacgcacatcacACgt............... : 1050555 77 : ron 5 >>>> >>>> Target Intron 6 >>>> ThrGluValAsnTyr : 80 bp 71254 bp |||:!!||| ! ++++ ++ThrLysValProGln 1050554 : ..........aggt.........................agACCAAGGTGCCACAG : 905492 81 : ThrGlnLeuValAspLeuHisAlaArgTyrAlaGluCysGlyLeuArg >>>> T : 97 |||! ! ! ||| ||| ..!||||||... ThrProAsnSerAspGluIleLysArgLysProIleAlaGlyLeuGlu++ 905491 : ACGCCAAACAGtgatgaaatcaaaagaaaaccaaTTGCCGgactggaggt...... : 905439 98 : arget Intron 7 >>>> IleLeuAlaPheProCysAsnGlnPheGlyLysGl : 108 14038 bp ::: !!.!|||||| :::... |||||| ++ValTrpGlyPheProSerSerSerValGlyLys-- 905438 : ...................aggtgtGGGGCTTTCCtagcagctcagttggtaaa-- : 891370 109 : nGluPro >>>> Target Intron 8 >>>> GlySerAsnGluGluIleLy : 117 |||||| 18373 bp |||||||||......::: -GluPro++ ++GlySerAsnSerSerLeuLe 891369 : -gaacctgt.........................agggatcgaactcaagtctcct : 872973 118 : sGluPhe{Al} >>>> Target Intron 9 >>>> {a}AlaGlyTyrAsnV : 125 ...:::{::} 20233 bp {!}:!!|||!:!!..! uHisTyr{Se}++ ++{r}SerGlyPheThrA 872972 : gcattac{ag}gt.........................ag{T}TCTGGCTTCACAG : 852716 126 : alLys >>>> Target Intron 10 >>>> PheAspMetPheSer >>>> : 132 .!||| 51569 bp ||| ...::! laLys++ ++AlaAspCysLeuThr++ 852715 : CAAAAgt..........................aggcagactgtttaacGgt.... : 801124 133 : Target Intron 11 >>>> LysIleCysValAsn >>>> Target Int : 137 4183 bp |||! ! :!!!!. 12585 ++LysSerThrIleLys++ 801123 : ......................agAAAAGCACGATAAAGgt............... : 796926 138 : ron 12 >>>> GlyAspAsp{Al} >>>> Target Intron 13 >>>> : 140 bp ||||||..!{ } 24413 bp ++GlyAspSer{Ar}++ ++ 796925 : ...........agGGAGACTCC{AG}gt..........................ag : 759921 141 : {a}HisProLeu{T} >>>> Target Intron 14 >>>> {rp}LysTrpM : 147 {!}||||||! !{ } 107162 bp {!!}:!!||| {g}HisProPro{G}++ ++{ly}GlnTrpG 759920 : {A}CACCCGCCA{G}gt..........................ag{GG}CAGTGGG : 652738 148 : etLysIleGlnProLysGlyLysGlyIleLeuGly >>>> Target Intron : 159 ! :!: |||:::||||||||| !||| 128606 bp luPheValLeuProGluGlyLysGlyGlnGlnGly-+ 652737 : AGTTTGTGctgccagaaggcaaggggcagcAGGGAat................... : 652700 160 : 15 >>>> AsnAlaIleLysTrpAsnPheThrLysPheLeuIle{A} >>>> T : 171 ... ...:::|||||| ...||||||:::{|} ++GluGlnAlaSerPheAsnPheIleSerPheLeuLeu{A}+- 652699 : .......aggagcaagcgtcttttaatttcatttcttttctctta{g}gg...... : 524057 172 : arget Intron 16 >>>> {sp}LysAsnGlyCysValVal{Ly} >>>> T : 178 33865 bp {||}......|||||| !:!!{ } ++{sp}SerGlyGlyCysSerLeu{Gl}++ 524056 : ....................ag{ac}agCGGAGGCTGCTCGCTA{GG}gt...... : 490170 179 : arget Intron 17 >>>> {s}ArgTyrGlyProMetGluGlu{Pr} >>>> : 186 1494 bp {!} ! ! !|||! |||.!.{||} ++{y}AlaAlaPheProArgGluAsn{Pr}++ 490169 : ....................ag{G}GCAGCTTTTCCAAGAGAGAAC{CC}gt.... : 488652 187 : Target Intron 18 >>>> {o}LeuValIle{Gl} >>>> Target In : 190 11531 bp {|}||||||! !{:!} 83210 ++{o}LeuValAsn{Ly}++ 488651 : ......................ag{A}CTGGTGAAC{AA}gt.............. : 477109 191 : tron 19 >>>> {u}LysAspLeuProHisTyr : 196 bp {!}|||!!: !||| !:!! ++{s}LysGluLysProSerHis 477108 : ............ag{A}AAAGAGAAACCAAGCCAC : 393883 vulgar: SPP00000007_1.0 10 196 . NIVO01030317.1:subseq(555646,1182752) 1130465 393882 - 127 M 17 51 S 0 1 5 0 2 I 0 805 3 0 2 S 1 2 M 6 18 5 0 2 I 0 28790 3 0 2 M 7 21 5 0 2 I 0 43209 3 0 2 M 18 54 S 0 2 5 0 2 I 0 6893 3 0 2 S 1 1 M 15 45 5 0 2 I 0 73795 3 0 2 5 0 2 I 0 71250 3 0 2 M 21 63 5 0 2 I 0 14034 3 0 2 M 11 33 G 1 0 M 2 6 5 0 2 I 0 18369 3 0 2 M 9 27 S 0 2 5 0 2 I 0 20229 3 0 2 S 1 1 M 6 18 5 0 2 I 0 51565 3 0 2 M 5 15 5 0 2 I 0 4179 3 0 2 M 5 15 5 0 2 I 0 12581 3 0 2 M 3 9 S 0 2 5 0 2 I 0 24409 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 107158 3 0 2 S 1 2 M 14 42 5 0 2 I 0 128602 3 0 2 M 12 36 S 0 1 5 0 2 I 0 33861 3 0 2 S 1 2 M 6 18 S 0 2 5 0 2 I 0 1490 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 11527 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 83206 3 0 2 S 1 1 M 6 18 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 393883 1130465 127 - . gene_id 14 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1130414 1130465 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1130414 1130465 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1130412 1130413 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1129605 1130413 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1129605 1129606 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1129585 1129604 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1129585 1129604 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1129583 1129584 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1100791 1129584 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1100791 1100792 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1100770 1100790 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1100770 1100790 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1100768 1100769 . - . intron_id 3 ; splice_site "Gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1057557 1100769 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1057557 1057558 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1057501 1057556 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1057501 1057556 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1057499 1057500 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1050604 1057500 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1050604 1050605 . - . intron_id 3 ; splice_site "at" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1050558 1050603 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1050558 1050603 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1050556 1050557 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1050557 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 905505 976758 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 905505 905506 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 905442 905504 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 905442 905504 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 905440 905441 . - . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 891404 905441 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 891404 891405 . - . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 891365 891403 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 891365 891403 . - . insertions 0 ; deletions 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 891363 891364 . - . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 872992 891364 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 872992 872993 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 872963 872991 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 872963 872991 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 872961 872962 . - . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 852730 872962 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 852730 852731 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 852711 852729 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 852711 852729 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 852709 852710 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 801142 852710 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 801142 801143 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 801127 801141 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 801127 801141 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 801125 801126 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 796944 801126 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 796944 796945 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 796929 796943 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 796929 796943 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 796927 796928 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 784344 796928 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 784344 784345 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 784333 784343 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 784333 784343 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 784331 784332 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 759920 784332 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 759920 759921 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 759909 759919 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 759909 759919 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 759907 759908 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 652747 759908 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 652747 652748 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 652703 652746 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 652703 652746 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 652701 652702 . - . intron_id 15 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 524097 652702 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 524097 524098 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 524060 524096 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 524060 524096 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 524058 524059 . - . intron_id 16 ; splice_site "gG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 490195 524059 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 490195 490196 . - . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 490173 490194 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 490173 490194 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 490171 490172 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 488679 490172 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 488679 488680 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 488655 488678 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 488655 488678 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 488653 488654 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 488654 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 393902 477111 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 393902 393903 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 393883 393901 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 393883 393901 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 393883 1130465 127 - . alignment_id 14 ; Query SPP00000007_1.0 ; Align 1130466 11 51 ; Align 1129603 29 18 ; Align 1100791 35 21 ; Align 1057557 42 54 ; Align 1050603 61 45 ; Align 905505 76 63 ; Align 891404 97 33 ; Align 891371 109 6 ; Align 872992 111 27 ; Align 852729 121 18 ; Align 801142 127 15 ; Align 796944 132 15 ; Align 784344 137 9 ; Align 759919 141 9 ; Align 652745 145 42 ; Align 524097 159 36 ; Align 490193 172 18 ; Align 488678 179 21 ; Align 477123 187 9 ; Align 393901 191 18 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 125 Query range: 7 -> 184 Target range: 1149341 -> 121464 8 : ArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeu >>>> Target Intron : 20 |||:!!! !:!!|||!.!:!!|||||| !!|||||| 107825 bp ArgValSerGluProGlyValLeuCysArgAlaLeu++ 1149341 : AGGGTGTCTGAGCCCGGAGTTCTCTGCAGGGCCCTGgt.................. : 1149303 21 : 1 >>>> AlaAlaProGlyLeuAlaGlyThrMetCysAlaSer{Ar} >>>> : 32 !.!..!||| !||| !!||||||:!:||| !! !!{||} -+GlyCysProLeuLeuProGlyThrLeuCysProPro{Ar}+- 1149302 : .......cgGGATGCCCTCTGCTCCCTGGAACACTCTGTCCTCCC{CG}gg..... : 1041440 33 : Target Intron 2 >>>> {g}AspAspTrpArg{Cy} >>>> Target I : 37 5055 bp {|} ||||||{ !} 12501 ++{g}ArgArgTrpArg{Gl}++ 1041439 : ....................ag{G}AGAAGATGGAGG{GG}gt............. : 1036370 38 : ntron 3 >>>> {s}AlaArgSerMetHisGluPheSerAlaLys >>>> Ta : 48 1 bp {!} !|||:!! ||||||||| ! ! ++{y}LeuArgAlaGluAlaGluPheSerGlnAla++ 1036369 : ............ag{c}ctTCGGGCCGAAGCAGAGTTCTCGCAGGCGgt....... : 911328 49 : rget Intron 4 >>>> AspIle{A} >>>> Target Intron 5 >>>> : 50 183193 bp ||| !{|} 47653 bp ++AspSer{A}++ 911327 : ..................agGATTCA{G}gt......................... : 728128 51 : {sp}Gly >>>> Target Intron 6 >>>> HisMetValAsnLeuAsp : 57 {||}||| 110494 bp |||::: ... !||| ++{sp}Gly++ +-HisLeuArgLysGluAsp 728127 : ag{at}ggggt.........................accatctccggaaAGAGGAT : 569963 58 : LysTyrArgGlyPhe{Va} >>>> Target Intron 7 >>>> {l}CysIl : 65 ! |||||||||.!.{..} 59234 bp {!}||| HisTyrArgGlyLeu{Th}++ ++{r}CysTy 569962 : CATTACAGAGGTCTA{AC}gt.........................ag{A}TGCTA : 510705 66 : eValThrAsnValAlaSerGlnUnkGlyLys >>>> Target Intron 8 >> : 76 !! !! !!:!:!! !!!!:!! ! !||| 69830 bp rGlyArgSerLeuHisThrLysArgGluLys++ 510704 : TGGGAGAAGTTTACACACGAAGCGGGAAAAGgt....................... : 510670 77 : >> ThrGluValAsnTyrThrGlnLeuValAspLeuHisAlaArgTyrAlaGluC : 93 ...|||::!!:: !:!! !:!! ! ! !:!! !!::!.!|||. ++ProSerValSerTrpGlnGluGlyLeuProGluSerSerSerTrpGlyGluA 510669 : ..agccctcggTGTCCTGGCAGGAGGGCCTTCCTGAGTCCTCTTCCTGGGGAGAGG : 440791 94 : ysGlyLeu{Ar} >>>> Target Intron 9 >>>> {g}IleLeuAlaPhe : 100 ..||| !{||} 64514 bp {|}:::|||:::... laGlyLys{Ar}++ ++{g}LeuLeuSerLeu 440790 : CAGGTAAG{AG}gt.........................ag{g}cttctttctctc : 376256 101 : ProCysAsnGlnPheGlyLysGlnGluPro >>>> Target Intron 10 >> : 111 ||| ... !||||||! :!!||| 24481 bp ProLeuArgLeuPheGlyIleValGlnPro-+ 376255 : cccctcaGGTTATTTGGAATCGTCCAGCCCtt........................ : 376221 112 : >> GlySerAsnGluGlu{Il} >>>> Target Intron 11 >>>> {e} : 116 ||||||||| !{:!} 53746 bp {:} ++GlySerAsnProAla{Le}++ ++{u} 376220 : ..agggctcgaaccctgcA{TT}gt..........................ag{G} : 297979 117 : LysGluPheAlaAlaGlyTyrAsnValLysPhe{As} >>>> Target Intro : 128 !..!! ! !||| !!::|||..! !!:!{ } 26933 bp GlyArgSerIleAlaThrTrpAsnThrAlaTyr{Ar}++ 297978 : GGGCGGTCCATAGCCACCTGGAACACAGCATAC{AG}gt................. : 297941 129 : n 12 >>>> {p}MetPheSerLys{Il} >>>> Target Intron 13 >> : 133 { }! .!!:!!|||{! } 1676 bp ++{g}ThrLeuAlaLys{Ar}++ 297940 : .........ag{G}ACCCTTGCAAAG{AG}gt........................ : 270993 134 : >> {e}CysValAsnGly{A} >>>> Target Intron 14 >>>> {sp} : 138 {!}|||..!|||..!{|} 64866 bp {||} ++{g}CysThrAsnSer{A}++ ++{sp} 270992 : ..ag{A}TGTACTAATTCA{G}gt..........................ag{AT} : 204439 139 : AspAlaHisProLeuTrpLysTrp{Me} >>>> Target Intron 15 >>>> : 147 ! !! |||:::|||{ } 13128 bp ProLysLeuLeuArgTrpArgTrp{**}++ 204438 : CCTAAGCTGctcaggtggcgctgg{tg}gt.......................... : 204409 148 : {t}LysIleGlnProLysGlyLysGlyIleLeuGlyAsn{Al} >>>> Targ : 160 { }... ::: ! !||| !||||||:!!{ } ++{*}SerAlaCysGlnGluAspProGlyTyrLeuGlyHis{Ar}++ 204408 : ag{a}tctgcctgccaagaagACCCAGGGTACCTGGGACAC{AG}gt......... : 191242 161 : et Intron 16 >>>> {a}IleLysTrpAsnPheThrLysPheLeuIleAspL : 172 69707 bp {!}! ||| !:!|||||| ! !! ! ! ! ++{g}ArgLysHisSerPheThrValAlaSerGlyLysP 191241 : .................ag{G}AGGAAACACAGCTTCACAGTGGCTTCTGGCAAGC : 121503 173 : ysAsnGlyCysValValLysArgTyrGlyProMetGlu : 184 !.!|||||| !||| !! ! ||||||:!::!! roThrGlyCysSerValProGlyGlnGlyProLeuGln 121502 : CCACTGGCTGCTCCGTCCCTGGCCAGGGTCCCCTCCAG : 121465 vulgar: SPP00000007_1.0 7 184 . NIVO01030317.1:subseq(555646,1182752) 1149341 121464 - 125 M 12 36 5 0 2 I 0 107821 3 0 2 M 12 36 S 0 2 5 0 2 I 0 5051 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 125007 3 0 2 S 1 1 M 10 30 5 0 2 I 0 183189 3 0 2 M 2 6 S 0 1 5 0 2 I 0 47649 3 0 2 S 1 2 M 1 3 5 0 2 I 0 110490 3 0 2 M 11 33 S 0 2 5 0 2 I 0 59230 3 0 2 S 1 1 M 12 36 5 0 2 I 0 69826 3 0 2 M 20 60 S 0 2 5 0 2 I 0 64510 3 0 2 S 1 1 M 14 42 5 0 2 I 0 24477 3 0 2 M 5 15 S 0 2 5 0 2 I 0 53742 3 0 2 S 1 1 M 11 33 S 0 2 5 0 2 I 0 26929 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 1672 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 64862 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 13124 3 0 2 S 1 1 M 12 36 S 0 2 5 0 2 I 0 69703 3 0 2 S 1 1 M 24 72 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 121465 1149341 125 - . gene_id 15 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1149306 1149341 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1149306 1149341 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1149304 1149305 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1041481 1149305 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1041481 1041482 . - . intron_id 0 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1041443 1041480 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1041443 1041480 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1041441 1041442 . - . intron_id 2 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1036388 1041442 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1036388 1036389 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1036373 1036387 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1036373 1036387 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1036371 1036372 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 911362 1036372 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 911362 911363 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 911331 911361 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 911331 911361 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 911329 911330 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 728138 911330 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 728138 728139 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 728131 728137 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 728131 728137 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 728129 728130 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 680478 728130 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 680478 680479 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 680473 680477 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 680473 680477 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 680471 680472 . - . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 569979 680472 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 569979 569980 . - . intron_id 5 ; splice_site "ac" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 569944 569978 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 569944 569978 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 569942 569943 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 510710 569943 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 510710 510711 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 510673 510709 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 510673 510709 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 510671 510672 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 440843 510672 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 440843 440844 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 440781 440842 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 440781 440842 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 440779 440780 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 376267 440780 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 376267 376268 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 376224 376266 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 376224 376266 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 376222 376223 . - . intron_id 10 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 351743 376223 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 351743 351744 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 351726 351742 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 351726 351742 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 351724 351725 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297980 351725 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297980 297981 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297944 297979 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297944 297979 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297942 297943 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 271011 297943 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 271011 271012 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 270996 271010 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 270996 271010 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 270994 270995 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 269320 270995 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 269320 269321 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 269306 269319 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 269306 269319 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 269304 269305 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 204440 269305 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 204440 204441 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 204412 204439 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 204412 204439 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 204410 204411 . - . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 191284 204411 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 191284 191285 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 191245 191283 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 191245 191283 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 191243 191244 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 121538 191244 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 121538 121539 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 121465 121537 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 121465 121537 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 121465 1149341 125 - . alignment_id 15 ; Query SPP00000007_1.0 ; Align 1149342 8 36 ; Align 1041481 20 36 ; Align 1036387 33 12 ; Align 911361 38 30 ; Align 728138 48 6 ; Align 680476 51 3 ; Align 569979 52 33 ; Align 510709 64 36 ; Align 440843 76 60 ; Align 376266 97 42 ; Align 351743 111 15 ; Align 297979 117 33 ; Align 271010 129 12 ; Align 269319 134 12 ; Align 204438 139 24 ; Align 191283 148 36 ; Align 121537 161 72 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 124 Query range: 3 -> 195 Target range: 1007334 -> 80855 4 : GlyArgLeuCysArgLeuLeuLys{P} >>>> Target Intron 1 >>>> : 12 |||...:::||| ::::::{ } 30509 bp GlyAsnValCysAlaCysValGlu{V}++ ++ 1007334 : ggaaacgtgtgtgcatgtgtagaa{g}gt.........................ag : 976802 13 : {ro}AlaLeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAla{G} >>>> Tar : 26 { !}||||||:!!! |||::!!!! ! !||| !! !|||{|} {al}AlaLeuValSerGlySerPheHisLeuProPheSerAla{G}++ 976801 : {tg}GCTCTGGTTTCAGGAAGTTTCCACCTCCCCTTTTCTGCA{G}gt........ : 976756 27 : get Intron 2 >>>> {ly}ThrMetCysAlaSerArg{A} >>>> Targe : 33 118770 bp {||}|||:!: !:!!.!!!:!{!} 1 ++{ly}ThrLeuLeuSerGlyLys{G}++ 976755 : .................ag{GA}ACGCTTCTTTCTGGGAAG{G}gt.......... : 857965 34 : t Intron 3 >>>> {sp}AspTrp{A} >>>> Target Intron 4 >>> : 36 1507 bp {!:}||||||{!} 29612 bp ++{lu}AspTrp{L}++ 857964 : ...............ag{aa}gactgg{a}gt........................ : 846449 37 : > {rg}CysAlaArgSerMetHis{G} >>>> Target Intron 5 >>>> : 43 {:!} !!.!||||||:!: ! {|} 19875 bp ++{ys}IleValArgSerLeuLys{G}-+ + 846448 : .ag{AG}ATTGTCAGGTCTTTAAAA{G}at.........................a : 796945 44 : {lu}PheSerAlaLys{A} >>>> Target Intron 6 >>>> {sp}Ile : 49 {||} !! !|||{!} 612 bp {!:}! ! +{lu}LysHisAspLys{G}++ ++{lu}Arg 796944 : g{AA}AAGCACGATAAA{G}gt.........................ag{AA}AGA : 796314 50 : AspGlyHisMetValAsnLeuAspLysTyrArgGlyPheVal{C} >>>> Targ : 64 ! ||| !! |||!!. !|||!:!:!!|||||||||:!!{ } GlyGlySerLysValLysAspAspArgHisArgGlyPheLeu{A}++ 796313 : GGAGGTTCTAAAGTAAAAGATGACAGACATAGGGGGTTCTTA{G}gt......... : 796266 65 : et Intron 7 >>>> {ys}IleValThrAsnValAlaSerGlnUnkGlyLysT : 76 17305 bp { !}! ! !:!! :!!||||||! ! ! ++{sp}AsnProSerLeuLeuAlaSerProGlyGlnAspL 796265 : ................ag{AT}AACCCTTCCCTGCTAGCATCCCCTGGCCAAGATT : 778928 77 : hrGluValAsnTyrThrGlnLeuValAspLeuHisAlaArgTyrAlaGluCysGly : 94 !:!!:!!...! !!:!||| ! ! !|||... !|||:!!..!.!.||| !! euGlnLeuGlyCysSerGlnThrSerLeuLeuArgLeuArgHisCysHisCysArg 778927 : TACAGCTAGGGTGCAGTCAGACCTCGCTTCTGAGACTCAGACACTGTCACTGCAGG : 778874 95 : LeuArgIleLeuAlaPheProCysAsnGlnPhe{G} >>>> Target Intron : 106 ||| !!! ! !||| !|||||| !!. {|} 9131 bp LeuGlyLysGlnAlaAspProCysAlaHisThr{G}+- 778873 : CTTGGAAAGCAGGCAGATCCCTGTGCACACACA{G}gg.................. : 778835 107 : 8 >>>> {ly}Lys{G} >>>> Target Intron 9 >>>> {ln}GluP : 110 {||}:::{:} 9418 bp {!!}|||| ++{ly}Arg{G}++ ++{lu}GluP 778834 : .......ag{gg}agg{g}gt.........................ag{ag}gagc : 760277 111 : roGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyr{As} >>>> Targ : 124 ||||| ......:!! !..!.!. ! !|||:!!{||} roGlyArgLeuHisSerValGlySerLeuArgLeuGlyHis{As}++ 760276 : ctggaaggctacattcTGTAGGGTCGCTGAGACTCGGACAC{AA}gt......... : 760231 125 : et Intron 10 >>>> {n}ValLys{Ph} >>>> Target Intron 11 : 127 11016 bp {|}... {!:} 19428 bp ++{n}ThrThr{Tr}++ 760230 : .................ag{c}accacc{tg}gt...................... : 749206 128 : >>>> {e}AspMetPheSerLysIleCysValAsn{G} >>>> Target Int : 137 {:}|||:!! ||| !:!!! !||||||{|} 115008 ++{p}AspLeuGluSerProLeuPheValAsn{G}++ 749205 : ....ag{G}GACCTGGAAAGTCCGCTTTTCGTGAAT{G}gt............... : 729749 138 : ron 12 >>>> {ly}AspAspAlaHisProLeuTrp{Ly} >>>> Target : 145 bp {||} !!|||:!! !||| !|||{ } 600 ++{ly}HisAspSerValProThrTrp{Gl}++ 729748 : ...........ag{GG}CATGACTCTGTCCCCACATGG{GG}gt............ : 614716 146 : Intron 13 >>>> {s}TrpMetLysIleGlnProLysGlyLys >>>> Tar : 155 84 bp { }||| ||||||!!.||| !|||||| +-{y}TrpGluLysIleHisProGlyGlyLys++ 614715 : ..............ac{C}TGGGAAAAGATACACCCCGGAGGAAAGgt........ : 554604 156 : get Intron 14 >>>> >>>> Target Intron 15 >>>> GlyIle : 156 30735 bp 188186 bp ||| ++++ ++GlyGln 554603 : ..................aggt..........................agGGGCAG : 335682 157 : LeuGlyAsnAlaIleLysTrpAsnPheThrLysPheLeu >>>> Target Int : 170 ||||||:!! ! !::!|||.!.! ! !! !|||||| 83563 LeuGlyAspGlnGlyArgTrpGluSerProGlyPheLeu++ 335681 : CTGGGCGACCAGGGACGATGGGAATCCCCAGGGTTCCTAgt............... : 335638 171 : ron 16 >>>> IleAspLysAsnGlyCysValValLys >>>> Target In : 179 bp :!:|||!:!||| ! ! ! !||| 91628 ++ValAspArgAsnThrLeuCysArgLys++ 335637 : ...........agGTGGACAGAAACACCCTCTGCAGGAAGgt.............. : 252048 180 : tron 17 >>>> ArgTyrGlyProMet >>>> Target Intron 18 >>> : 184 bp !:!:!!|||||| 38272 bp ++LysHisGlyProSer++ 252047 : ............agAAGCACGGCCCATCTgt......................... : 160405 185 : > GluGluProLeuValIleGlu{Ly} >>>> Target Intron 19 >>>> : 191 |||.!.|||! !!..::::::{!:} 19072 bp ++GluHisProSerAlaLeuLys{Ar}+- 160404 : .aggagCATCCCTCAGccctgaaa{ag}ga.......................... : 122110 192 : {s}AspLeu{Pr} >>>> Target Intron 20 >>>> {o}His : 195 {!}||||||{||} 22172 bp {|}||| ++{g}AspLeu{Pr}++ ++{o}His 122109 : ag{G}GACCTC{CC}gt..........................ag{A}CAC : 80856 vulgar: SPP00000007_1.0 3 195 . NIVO01030317.1:subseq(555646,1182752) 1007334 80855 - 124 M 8 24 S 0 1 5 0 2 I 0 30505 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 118766 3 0 2 S 1 2 M 6 18 S 0 1 5 0 2 I 0 11503 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 29608 3 0 2 S 1 2 M 6 18 S 0 1 5 0 2 I 0 19871 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 608 3 0 2 S 1 2 M 15 45 S 0 1 5 0 2 I 0 17301 3 0 2 S 1 2 M 41 123 S 0 1 5 0 2 I 0 9127 3 0 2 S 1 2 M 1 3 S 0 1 5 0 2 I 0 9414 3 0 2 S 1 2 M 15 45 S 0 2 5 0 2 I 0 11012 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 19424 3 0 2 S 1 1 M 9 27 S 0 1 5 0 2 I 0 115004 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 60080 3 0 2 S 1 1 M 9 27 5 0 2 I 0 30731 3 0 2 5 0 2 I 0 188182 3 0 2 M 15 45 5 0 2 I 0 83559 3 0 2 M 9 27 5 0 2 I 0 91624 3 0 2 M 5 15 5 0 2 I 0 38268 3 0 2 M 7 21 S 0 2 5 0 2 I 0 19068 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 22168 3 0 2 S 1 1 M 1 3 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 80856 1007334 124 - . gene_id 16 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1007310 1007334 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1007310 1007334 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1007308 1007309 . - . intron_id 1 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976801 1007309 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976801 976802 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 976759 976800 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 976759 976800 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 857989 976758 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 857989 857990 . - . intron_id 1 ; splice_site "aG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 857968 857988 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 857968 857988 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 857966 857967 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 846461 857967 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 846461 846462 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 846452 846460 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 846452 846460 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 846450 846451 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 816840 846451 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 816840 816841 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 816819 816839 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 816819 816839 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 816817 816818 . - . intron_id 5 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 796944 816818 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 796944 796945 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 796929 796943 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 796929 796943 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 796927 796928 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 796317 796928 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 796317 796318 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 796269 796316 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 796269 796316 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 796267 796268 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 778964 796268 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 778964 778965 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 778838 778963 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 778838 778963 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 778836 778837 . - . intron_id 8 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 769707 778837 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 769707 769708 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 769701 769706 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 769701 769706 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 769699 769700 . - . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 760283 769700 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 760283 760284 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 760234 760282 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 760234 760282 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 760232 760233 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 749218 760233 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 749218 749219 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 749209 749217 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 749209 749217 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 749207 749208 . - . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 729781 749208 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 729781 729782 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 729752 729780 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 729752 729780 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 729750 729751 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 614744 729751 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 614744 614745 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 614719 614743 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 614719 614743 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 614717 614718 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 554635 614718 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 554635 554636 . - . intron_id 12 ; splice_site "AC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 554607 554634 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 554607 554634 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 554605 554606 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 554606 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 335686 523871 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 335686 335687 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 335641 335685 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 335641 335685 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 335639 335640 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 252078 335640 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 252078 252079 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 252051 252077 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 252051 252077 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 252049 252050 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 160423 252050 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 160423 160424 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 160408 160422 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 160408 160422 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 160406 160407 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 122136 160407 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 122136 122137 . - . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 122113 122135 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 122113 122135 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 122111 122112 . - . intron_id 19 ; splice_site "ga" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 103041 122112 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 103041 103042 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 103032 103040 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 103032 103040 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 103030 103031 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 80860 103031 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 80860 80861 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 80856 80859 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 80856 80859 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 80856 1007334 124 - . alignment_id 16 ; Query SPP00000007_1.0 ; Align 1007335 4 24 ; Align 976799 13 39 ; Align 857987 27 18 ; Align 846459 34 6 ; Align 816838 37 18 ; Align 796942 44 12 ; Align 796315 49 45 ; Align 778962 65 123 ; Align 769705 107 3 ; Align 760281 109 45 ; Align 749217 125 6 ; Align 729780 128 27 ; Align 614742 138 21 ; Align 554634 146 27 ; Align 335686 155 45 ; Align 252078 170 27 ; Align 160423 179 15 ; Align 122136 184 21 ; Align 103040 192 6 ; Align 80859 195 3 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 123 Query range: 10 -> 194 Target range: 1154516 -> 78861 11 : LysProAlaLeuLeuCysGlyAlaLeuAla >>>> Target Intron 1 >>> : 21 ..!||||||!!!:!!||||||::!! !! ! 36488 bp SerProAlaPheValCysGlySerSerGlu++ 1154516 : TCACCTGCCTTTGTCTGCGGCAGCTCTGAGgt........................ : 1154484 22 : > AlaProGlyLeuAlaGlyThr{Me} >>>> Target Intron 2 >>>> : 28 .!!|||!.!! !::! !|||{! } 78832 bp ++ThrProAlaCysSerThrThr{Ar}++ + 1154483 : .agACCCCTGCATGCAGCACCACA{AG}gt.........................a : 1039145 29 : {t}CysAlaSerArg >>>> Target Intron 3 >>>> AspAspTrpAr : 36 {!}|||!.!|||!.! 55525 bp !!:! || +{g}CysValSerHis++ ++GluGlyIleAr 1039144 : g{G}TGTGTTTCCCACgt.........................agGAAGGGATCCG : 983596 37 : gCysAlaArgSerMetHisGluPheSerAlaLys{A} >>>> Target Intro : 48 |||||||||||||! .!.! |||..!!.! {|} 99446 bp gCysAlaArgSerArgGluAlaPheGlnGlyPhe{A}+- 983595 : GTGCGCGCGGTCCAGAGAGGCCTTCCAGGGCTTC{G}gg................. : 983557 49 : n 4 >>>> {sp}IleAspGlyHisMetValAsnLeuAspLysTyrArgGlyPhe : 62 {||} ...... ||| !..!:!!!::!.!..! !! ++{sp}ThrHisThrGluGlnArgAsnGluSerGluTrpAsnSerVal 983556 : ........ag{ac}acacacacagagcagagaaATGAATCCGAGTGGAATTCCGTT : 884072 63 : ValCysIleValThrAsnValAlaSerGlnUnkGlyLysThr{G} >>>> Targ : 77 |||||| ! !!:!:::||| ! !!.!. !:!!|||{!} ValCysSerProSerSerValLysProAspArgThrGlnThr{A}++ 884071 : GTGTGTTCACCAAGTTCAGTGAAGCCCGACCGCACACAGACG{G}gt......... : 884024 78 : et Intron 5 >>>> {lu}ValAsnTyrThrGlnLeu{Va} >>>> Targe : 84 929 bp { }:::... |||:::|||{ } 2 ++{la}LeuGlyThrThrArgLeu{Ar}++ 884023 : ................ag{cc}ctggggaccacacgtctg{ag}gt.......... : 883073 85 : t Intron 6 >>>> {l}AspLeuHisAlaArg{Ty} >>>> Target Int : 90 9936 bp {!}||| !||| !|||{ } 184021 ++{g}AspLysHisGlnArg{Il}++ 883072 : ...............ag{g}GACAAACACCAAAGA{AT}gt............... : 853119 91 : ron 7 >>>> {r}AlaGluCysGlyLeuArgIle >>>> Target Intron : 98 bp { }!.!.!. !||||||||| 51741 bp ++{e}ValHisValGlyLeuArgPro++ 853118 : ..........ag{A}GTCCACGTCGGTCTCCGCCCGgt.................. : 669076 99 : 8 >>>> LeuAlaPheProCysAsnGlnPheGly{L} >>>> Target Int : 107 ||| !.!!||| !!.. !!!.|||{|} 39581 ++LeuLeuIleProAsnThrAlaLeuGly{L}++ 669075 : .......agCTCCTAATTCCCAACACAGCGTTGGGA{A}gt............... : 617307 108 : ron 9 >>>> {ys} >>>> Target Intron 10 >>>> GlnGluProG : 111 bp {||} 8894 bp |||! !|||| ++{ys}++ ++GlnAlaProG 617306 : ..........ag{AG}gt..........................agCAGGCACCCG : 568823 112 : lySerAsnGluGluIleLysGluPheAlaAlaGly{Ty} >>>> Target Int : 123 ||:!! ! !:!:|||!!:.!.|||!.!!.!{!:} 22737 lyAlaValThrValLeuLysAspIleAlaGlyAla{Tr}++ 568822 : GCGCTGTAACCGTGTTGAAGGATATAGCTGGAGCA{TG}gt............... : 568783 124 : ron 11 >>>> {r}AsnVal{Ly} >>>> Target Intron 12 >>>> : 126 bp {:}!:! !{!.} 76554 bp ++{p}SerCys{Se}++ ++ 568782 : ...........ag{G}AGCTGC{AG}gt..........................ag : 469487 127 : {s}PheAspMetPheSerLys{I} >>>> Target Intron 13 >>>> {l : 133 {.} ! :!: !|||::!{ } 16239 bp { {r}HisArgLeuAspSerArg{A}++ ++{s 469486 : {T}CACAGGCTAGATTCTCGA{G}gt..........................ag{A : 453226 134 : e}CysValAsnGlyAspAspAlaHisProLeuTrpLysTrpMetLysIleGlnPro : 151 !}|||! !!:! !:!!! :!! !|||!!!||| ! ! !:!: ||| p}CysGlySerLysAsnAlaSerPheProPheTrpLeuValTyrLeuLeuAlaPro 453225 : C}TGTGGCAGCAAGAACGCGTCCTTCCCGTTCTGGCTGGTGTACCTGCTGGCCCCG : 453173 152 : LysGlyLysGlyIleLeu >>>> Target Intron 14 >>>> GlyAsnAl : 160 ||| ! !:!!||| 101392 bp |||||| LeuGlyAlaLeuLeuLeu++ ++GlyAsnPh 453172 : CTCGGGGCGCTGCTCCTGgt..........................aggggaactt : 351754 161 : aIleLysTrpAsnPheThrLysPhe{Le} >>>> Target Intron 15 >>> : 169 ::: ... ... {||} 2209 bp eLeuThrGlnGlySerAsnProAla{Le}++ 351753 : cctgacccagggctcgaaccctgcA{TT}gt......................... : 351723 170 : > {u}IleAspLysAsnGlyCysValVal{L} >>>> Target Intron 16 : 178 {|}||| !!..!..||||||||||||{!} 43213 bp ++{u}IleCysSerThrGlyCysValVal{A}++ 351722 : .ag{A}ATCTGTAGCACAGGCTGTGTTGTC{A}gt..................... : 349488 179 : >>>> {ys}ArgTyr{G} >>>> Target Intron 17 >>>> {ly}Pr : 182 {:!}||| {|} 35258 bp {||}|| ++{rg}ArgAla{G}++ ++{ly}Pr 349487 : .....ag{GG}Agagca{g}gt..........................ag{GA}CC : 271008 183 : oMetGluGlu{P} >>>> Target Intron 18 >>>> {ro}LeuValIle : 189 |:!!:!!..!{ } 192108 bp {!!}||| !!:!! oLeuGlnArg{A}++ ++{la}LeuPheLeu 271007 : CTTGCAAAGA{G}gt..........................ag{CC}CTGTTTCTC : 78879 190 : GluLysAspLeuPro : 194 ...||| !|||||| SerLysProLeuPro 78878 : TCCAAGCCTCTGCCC : 78862 vulgar: SPP00000007_1.0 10 194 . NIVO01030317.1:subseq(555646,1182752) 1154516 78861 - 123 M 10 30 5 0 2 I 0 36484 3 0 2 M 7 21 S 0 2 5 0 2 I 0 78828 3 0 2 S 1 1 M 4 12 5 0 2 I 0 55521 3 0 2 M 15 45 S 0 1 5 0 2 I 0 99442 3 0 2 S 1 2 M 28 84 S 0 1 5 0 2 I 0 925 3 0 2 S 1 2 M 6 18 S 0 2 5 0 2 I 0 29932 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 184017 3 0 2 S 1 1 M 7 21 5 0 2 I 0 51737 3 0 2 M 9 27 S 0 1 5 0 2 I 0 39577 3 0 2 S 1 2 5 0 2 I 0 8890 3 0 2 M 15 45 S 0 2 5 0 2 I 0 22733 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 76550 3 0 2 S 1 1 M 6 18 S 0 1 5 0 2 I 0 16235 3 0 2 S 1 2 M 24 72 5 0 2 I 0 101388 3 0 2 M 11 33 S 0 2 5 0 2 I 0 2205 3 0 2 S 1 1 M 8 24 S 0 1 5 0 2 I 0 43209 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 35254 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 192104 3 0 2 S 1 2 M 8 24 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 78862 1154516 123 - . gene_id 17 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1154487 1154516 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1154487 1154516 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1154485 1154486 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1117999 1154486 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1117999 1118000 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1117976 1117998 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1117976 1117998 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1117974 1117975 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1039144 1117975 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1039144 1039145 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1039131 1039143 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1039131 1039143 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1039129 1039130 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 983606 1039130 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 983606 983607 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 983560 983605 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 983560 983605 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 983558 983559 . - . intron_id 4 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 884114 983559 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 884114 884115 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 884027 884113 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 884027 884113 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 884025 884026 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 883098 884026 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 883098 883099 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 883076 883097 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 883076 883097 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 883074 883075 . - . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 853140 883075 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 853140 853141 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 853122 853139 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 853122 853139 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 853120 853121 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 669101 853121 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 669101 669102 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 669079 669100 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 669079 669100 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 669077 669078 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 617338 669078 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 617338 617339 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 617310 617337 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 617310 617337 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 617308 617309 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 577729 617309 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 577729 577730 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 577727 577728 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 577727 577728 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 577725 577726 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 568833 577726 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 568833 568834 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 568786 568832 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 568786 568832 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 568784 568785 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 568785 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 469486 546039 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 469486 469487 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 469466 469485 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 469466 469485 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 469464 469465 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 453227 469465 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 453227 453228 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 453153 453226 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 453153 453226 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 453151 453152 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 351761 453152 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 351761 351762 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 351726 351760 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 351726 351760 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 351724 351725 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 349517 351725 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 349517 349518 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 349491 349516 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 349491 349516 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 349489 349490 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 306278 349490 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 306278 306279 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 306269 306277 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 306269 306277 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 306267 306268 . - . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 271011 306268 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 271011 271012 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 270996 271010 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 270996 271010 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 270994 270995 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 78888 270995 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 78888 78889 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 78862 78887 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 78862 78887 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 78862 1154516 123 - . alignment_id 17 ; Query SPP00000007_1.0 ; Align 1154517 11 30 ; Align 1117999 21 21 ; Align 1039143 29 12 ; Align 983606 33 45 ; Align 884112 49 84 ; Align 883096 78 18 ; Align 853139 85 15 ; Align 669100 91 21 ; Align 617338 98 27 ; Align 568833 108 45 ; Align 546048 124 6 ; Align 469485 127 18 ; Align 453225 134 72 ; Align 351761 158 33 ; Align 349516 170 24 ; Align 306276 179 6 ; Align 271009 182 12 ; Align 78886 187 24 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 118 Query range: 2 -> 195 Target range: 1180097 -> 38126 3 : LeuGlyArgLeu{C} >>>> Target Intron 1 >>>> {ys}ArgLeuLe : 10 ||||||! !|||{.} 38286 bp {.!}|||||||| LeuGlyThrLeu{A}++ ++{la}ArgLeuLe 1180097 : CTGGGGACCCTG{G}gt.........................ag{CC}CGGCTTCT : 1141790 11 : uLysProAlaLeu{L} >>>> Target Intron 2 >>>> {eu}CysGlyA : 18 |::!|||:!! !{!} 12054 bp { !} |||| uArgProSerAla{A}++ ++{rg}ThrGlyA 1141789 : CCGGCCATCCGCA{C}gt.........................ag{GG}ACAGGCG : 1129712 19 : la >>>> Target Intron 3 >>>> LeuAlaAlaProGly{L} >>>> : 24 || 34158 bp !|||!.!||||||{ } la++ ++LysAlaValProGly{G}++ 1129711 : CTgt.........................agAAGGCAGTCCCAGGA{G}gt..... : 1095533 25 : Target Intron 4 >>>> {eu}AlaGlyThrMetCysAla{S} >>>> Ta : 31 40002 bp { !} |||||||||{!} ++{ly}IleGluProMetCysAla{A}++ 1095532 : ....................ag{gg}atcgaacccatgtgtgca{a}gt....... : 1055510 32 : rget Intron 5 >>>> {er}ArgAspAsp >>>> Target Intron 6 : 35 71907 bp {!!}|||||| 6836 bp ++{rg}ArgAspPro++ 1055509 : ..................ag{GA}AGGGATCCGgt..................... : 983592 36 : >>>> >>>> Target Intron 7 >>>> TrpArgCysAlaArgSerMet : 41 73823 bp |||||| !! ! !|||:!! ++++ -+TrpArgSerArgAlaSerLeu 983591 : ....aggt.........................ggTGGAGAAGTAGGGCATCCTTG : 902917 42 : HisGluPheSerAlaLysAspIleAspGlyHisMet >>>> Target Intron : 54 .!...! !|||:!!:!!!!::!: |||||| ! 41674 bp GluArgHisSerSerGlnGluValArgGlyHisTrp++ 902916 : GAAAGGCATTCGTCTCAGGAGGTGAGAGGGCATTGGgt.................. : 902876 55 : 8 >>>> ValAsnLeuAspLysTyr{A} >>>> Target Intron 9 >>> : 60 !:!!||| !!.|||{ } 33702 bp ++SerHisLeuLeuAsnTyr{G}++ 902875 : .......agTCCCATTTATTAAATTAC{G}gt........................ : 861183 61 : > {rg}GlyPheValCysIleValThrAsnValAlaSerGln{U} >>>> Tar : 73 {!!}!.! ! |||::: :::...:::...:::...{ } ++{ly}AlaValPheCysLeuGlySerLysIleThrAlaAsp{G}++ 861182 : .ag{GG}GCTGTtttttgcttgggctccaaaatcactgcagac{g}gt........ : 827442 74 : get Intron 10 >>>> {nk}GlyLysThrGluValAsnTyrThrGlnLeuVa : 84 17972 bp { } ::: |||... !!|||... :: ++{ly}IleGluProGluThrTyrTyrValAlaCysIl 827441 : ..................ag{gg}attgaacctgagactTACTATGtagcctgcat : 809440 85 : lAsp{Le} >>>> Target Intron 11 >>>> {u}HisAlaArgTyr{Al : 91 : { } 12489 bp {!} ! ||||||!! {:: eGly{Ar}++ ++{g}LysAlaArg***{Se 809439 : tggc{ag}gt..........................ag{A}AAAGCACGATAA{AG : 796930 92 : } >>>> Target Intron 12 >>>> {a}GluCysGlyLeuArgIleLeu{ : 99 } 38488 bp {!}||||||||||||!! { }++ ++{r}GluCysGlyLeuSerSerGlu{ 796929 : }gt..........................ag{T}GAGTGTGGTCTGAGtagtgag{ : 758418 100 : Al} >>>> Target Intron 13 >>>> {a}PheProCysAsnGlnPheGl : 106 !.} 22430 bp {!}||||||! !...:!! .! Gl}++ ++{y}PheProSerGlyGluGluSe 758417 : gg}gt..........................ag{G}TTCCCCTCTGGAGAGGAGAG : 735967 107 : yLysGlnGluPro >>>> Target Intron 14 >>>> GlySerAsnGluG : 115 ! |||...||| 75459 bp |||||||||::: rGlyGlnSerPro+- ++GlySerAsnGlnG 735966 : CGGCCAGTCCCCAga..........................agggatcgaaccagg : 660481 116 : luIleLysGluPheAla >>>> Target Intron 15 >>>> >>>> Ta : 121 ::: ...::: 136592 bp lyLeuLeuHisTrpGln++ ++++ 660480 : gtctcctgcattggcaggt..........................aggt....... : 523869 122 : rget Intron 16 >>>> AlaGlyTyrAsn >>>> Target Intron 17 : 125 46748 bp .!!||| ! .!. 7632 bp ++ThrGlyGluGln++ 523868 : ...................agACTGGTGAACAAgt..................... : 477109 126 : >>>> ValLysPheAsp{Me} >>>> Target Intron 18 >>>> {t} : 129 !.!!:!|||...{! } 64366 bp {!} ++AlaArgPheSer{Ar}++ ++{g} 477108 : .....agGCTAGATTCTCG{AG}gt..........................ag{G} : 405099 130 : PheSerLysIleCysValAsn{G} >>>> Target Intron 19 >>>> {l : 137 !!!!!.||||||! !...{|} 26121 bp {| ArgCysAsnIleCysGlyGly{G}++ ++{l 405098 : AGATGTAACATCTGTGGTGGG{G}gt..........................ag{g : 378955 138 : y}AspAspAla >>>> Target Intron 20 >>>> HisPro >>>> Ta : 143 |}|||...... 77748 bp |||||| y}AspSerVal++ ++HisPro++ 378954 : t}gactcagtggt..........................agCACCCAgt....... : 301188 144 : rget Intron 21 >>>> LeuTrpLysTrpMetLysIleGlnProLys{G} : 153 119971 bp !||| ! :!!|||!:!{|} ++AlaTrpProGlyAlaValTrpLysProArg{G}-+ 301187 : ...................agGCCTGGCCTGGCGCAGTCTGGAAGCCTAGA{G}ct : 181188 154 : >>>> Target Intron 22 >>>> {ly}Lys{G} >>>> Target Intr : 155 25059 bp {||}|||{|} 57980 b ++{ly}Lys{G}++ 181187 : ..........................ag{ga}aaa{g}gt................ : 156121 156 : on 23 >>>> {ly}IleLeuGlyAsnAlaIleLysTrpAsnPheThrLysPheL : 169 p {||} |||||| :::::::::::: ||| | ++{ly}GlyHisGlyAsnProLeuGlnTyrSerCysArgLysAsnL 156120 : ..........ag{ga}gggcacggcaacccactccagtattcttgccggaagaatc : 98102 170 : euIleAspLysAsnGly{Cy} >>>> Target Intron 24 >>>> {s}Va : 176 ||:::|||:::... {||} 31075 bp {|} euMetAspArgGlyThr{Cy}++ ++{s}Ar 98101 : tcatggacagaggaacc{tg}gt..........................ag{C}CG : 67006 177 : lValLysArg >>>> Target Intron 25 >>>> TyrGlyProMetGluG : 185 !!.! ! ||| 15238 bp !!||||||.!.|||: gAlaHisArg++ ++AspGlyProPheGluG 67005 : GGCACATCGGgt..........................agGATGGCCCCTTTGAGC : 51741 186 : luPro >>>> Target Intron 26 >>>> >>>> Target Intron : 187 !!! ! 9787 bp 3795 bp lnGln++ ++++ 51740 : AGCAGgt..........................aggt................... : 41946 188 : 27 >>>> LeuValIleGluLysAspLeuProHis : 195 ! !||| !|||.!.!!!|||||| ++AlaGlnIleThrLysGlnPheProHis 41945 : .......agGCACAAATTACAAAACAGTTTCCCCAC : 38127 vulgar: SPP00000007_1.0 2 195 . NIVO01030317.1:subseq(555646,1182752) 1180097 38126 - 118 M 4 12 S 0 1 5 0 2 I 0 38282 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 12050 3 0 2 S 1 2 M 3 9 5 0 2 I 0 34154 3 0 2 M 5 15 S 0 1 5 0 2 I 0 39998 3 0 2 S 1 2 M 6 18 S 0 1 5 0 2 I 0 71903 3 0 2 S 1 2 M 3 9 5 0 2 I 0 6832 3 0 2 5 0 2 I 0 73819 3 0 2 M 19 57 5 0 2 I 0 41670 3 0 2 M 6 18 S 0 1 5 0 2 I 0 33698 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 17968 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 12485 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 38484 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 22426 3 0 2 S 1 1 M 11 33 5 0 2 I 0 75455 3 0 2 M 10 30 5 0 2 I 0 136588 3 0 2 5 0 2 I 0 46744 3 0 2 M 4 12 5 0 2 I 0 7628 3 0 2 M 4 12 S 0 2 5 0 2 I 0 64362 3 0 2 S 1 1 M 7 21 S 0 1 5 0 2 I 0 26117 3 0 2 S 1 2 M 3 9 5 0 2 I 0 77744 3 0 2 M 2 6 5 0 2 I 0 119967 3 0 2 M 10 30 S 0 1 5 0 2 I 0 25055 3 0 2 S 1 2 M 1 3 S 0 1 5 0 2 I 0 57976 3 0 2 S 1 2 M 19 57 S 0 2 5 0 2 I 0 31071 3 0 2 S 1 1 M 4 12 5 0 2 I 0 15234 3 0 2 M 7 21 5 0 2 I 0 9783 3 0 2 5 0 2 I 0 3791 3 0 2 M 9 27 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 38127 1180097 118 - . gene_id 18 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1180085 1180097 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1180085 1180097 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1180083 1180084 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1141799 1180084 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1141799 1141800 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1141775 1141798 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1141775 1141798 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1141773 1141774 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1129721 1141774 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1129721 1129722 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1129710 1129720 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1129710 1129720 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1129708 1129709 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1095552 1129709 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1095552 1095553 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1095536 1095551 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1095536 1095551 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1095534 1095535 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1055534 1095535 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1055534 1055535 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1055513 1055533 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1055513 1055533 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1055511 1055512 . - . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 983606 1055512 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 983606 983607 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 983595 983605 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 983595 983605 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 983593 983594 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 983594 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 902936 976758 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 902936 902937 . - . intron_id 6 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 902879 902935 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 902879 902935 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 902877 902878 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 861205 902878 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 861205 861206 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 861186 861204 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 861186 861204 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 861184 861185 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 827484 861185 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 827484 827485 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 827445 827483 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 827445 827483 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 827443 827444 . - . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 809473 827444 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 809473 809474 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 809433 809472 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 809433 809472 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 809431 809432 . - . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 796944 809432 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 796944 796945 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 796929 796943 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 796929 796943 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 796927 796928 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 758441 796928 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 758441 758442 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 758417 758440 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 758417 758440 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 758415 758416 . - . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 735987 758416 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 735987 735988 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 735953 735986 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 735953 735986 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 735951 735952 . - . intron_id 14 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 660494 735952 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 660494 660495 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 660464 660493 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 660464 660493 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 660462 660463 . - . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 660463 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 523871 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 469480 477111 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 469480 469481 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 469466 469479 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 469466 469479 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 469464 469465 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 405100 469465 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 405100 405101 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 405077 405099 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 405077 405099 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 405075 405076 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 378956 405076 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 378956 378957 . - . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 378945 378955 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 378945 378955 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 378943 378944 . - . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 301197 378944 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 301197 301198 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 301191 301196 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 301191 301196 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 301189 301190 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 181220 301190 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 181220 181221 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 181189 181219 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 181189 181219 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 181187 181188 . - . intron_id 22 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 156130 181188 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 156130 156131 . - . intron_id 21 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 156124 156129 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 156124 156129 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 156122 156123 . - . intron_id 23 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 98144 156123 . - . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 98144 98145 . - . intron_id 22 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 98083 98143 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 98083 98143 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 98081 98082 . - . intron_id 24 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 67008 98082 . - . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 67008 67009 . - . intron_id 23 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 66995 67007 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 66995 67007 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 66993 66994 . - . intron_id 25 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 51757 66994 . - . intron_id 25 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 51757 51758 . - . intron_id 24 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 51736 51756 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 51736 51756 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 51734 51735 . - . intron_id 26 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 51735 . - . intron_id 26 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 25 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 27 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 38154 41948 . - . intron_id 27 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 38154 38155 . - . intron_id 26 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 38127 38153 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 38127 38153 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 38127 1180097 118 - . alignment_id 18 ; Query SPP00000007_1.0 ; Align 1180098 3 12 ; Align 1141797 8 21 ; Align 1129719 16 9 ; Align 1095552 19 15 ; Align 1055532 25 18 ; Align 983604 32 9 ; Align 902936 35 57 ; Align 861205 54 18 ; Align 827482 61 36 ; Align 809471 74 36 ; Align 796943 87 12 ; Align 758440 92 21 ; Align 735986 100 33 ; Align 660494 111 30 ; Align 477124 121 12 ; Align 469480 125 12 ; Align 405099 130 21 ; Align 378954 138 9 ; Align 301197 141 6 ; Align 181220 143 30 ; Align 156128 154 3 ; Align 98142 156 57 ; Align 67007 176 12 ; Align 51757 180 21 ; Align 38154 187 27 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 118 Query range: 4 -> 153 Target range: 934285 -> 4959 5 : ArgLeuCysArgLeuLeu{L} >>>> Target Intron 1 >>>> {ys}ProA : 13 |||:!!||||||:!!! !{:} 89834 bp {!!}||| ArgValCysArgMetPro{G}++ ++{lu}ProP 934285 : AGGGTCTGCAGAATGCCA{G}gt.........................ag{AA}CCAC : 844427 14 : la{L} >>>> Target Intron 2 >>>> {eu}LeuCysGlyAlaLeuAlaAl : 21 !!{:} 13156 bp {!!} !..!|||::!:!!! !|| ro{V}++ ++{al}ThrAlaGlySerValGluAl 844426 : CA{G}gt.........................ag{TC}ACTGCCGGGAGTGTGGAGGC : 831247 22 : a >>>> Target Intron 3 >>>> Pro{G} >>>> Target Intron 4 : 23 | 167666 bp |||{|} 117526 bp a++ ++Pro{G}++ 831246 : Ggt.........................agCCT{G}gt.................... : 663572 24 : >>>> {ly}LeuAla{G} >>>> Target Intron 5 >>>> {ly}ThrMe : 28 {||} !|||{|} 92583 bp {||}|||.! ++{ly}AlaAla{G}++ ++{ly}ThrPh 663571 : .....ag{GA}GCTGCA{G}gt.........................ag{gc}acaTT : 453451 29 : tCysAlaSerArgAspAspTrp{Ar} >>>> Target Intron 6 >>>> {g} : 36 .|||||| !! !..! !|||{ !} 14965 bp {!} eCysAlaProAspSerIleTrp{Cy}++ +-{s} 453450 : TTGTGCGCCAGACTCCATCTGG{TG}gt.........................aa{c} : 438460 37 : CysAlaArgSerMetHisGluPhe >>>> Target Intron 7 >>>> SerAl : 46 ||| ..!|||...|||||| 121921 bp ! !|| CysLysGluSerGlnHisGluLys+- ++TyrAl 438459 : tgcaaagaGTCCCAACACGAAAAGga.........................agTATGC : 316511 47 : aLysAspIleAsp{G} >>>> Target Intron 8 >>>> {ly}HisMetVal : 54 |:!!!!::!! {|} 186209 bp {||} !|||:!! aGluGluValPro{G}++ ++{ly}SerMetLeu 316510 : AGAGGAAGTCCCA{G}gt.........................ag{GC}AGCATGCTC : 130278 55 : AsnLeuAspLysTyrArg{Gl} >>>> Target Intron 9 >>>> {y}PheV : 63 ::!|||||| ! !|||{||} 65933 bp {|}!:! SerLeuAspProSerArg{Gl}++ ++{y}TyrG 130277 : TCCCTGGATCCCTCCAGA{GG}gt.........................ag{C}TACC : 64318 64 : al >>>> Target Intron 10 >>>> CysIleValThrAsnValAlaSerGl : 72 ! 4929 bp |||:!:||| ...::: ||| ln++ ++CysValValIleGlyLeuGlnSerTh 64317 : AGgt..........................agTGTGTGGTCatcgggctgcagtccac : 59362 73 : n{U} >>>> Target Intron 11 >>>> {nk}GlyLysThrGluValAsn : 80 { } 31816 bp { }|||:::||||||...... r{G}++ ++{ly}GlyGluThrGluAlaGlu++ 59361 : a{g}gt..........................ag{gt}ggggagactgaggcagaggt : 27522 81 : >>>> Target Intron 12 >>>> TyrThrGlnLeuValAspLeuHisAlaArg : 89 8828 bp |||!!. !:!! !!||| ! ! !! ++MetThrHisSerMetTyrLeuIleAsnGly 27521 : ..........................agatgaCACACAGCATGTATTTAATCAATGGA : 18667 90 : TyrAlaGluCysGlyLeuArg >>>> Target Intron 13 >>>> Ile >> : 98 :!!::!..!|||..!:!!!:! 6674 bp :!: HisSerArgCysSerIleLys++ ++Val++ 18666 : CACAGTAGATGTTCTATAAAGgt..........................agGTGgt.. : 11964 99 : >> Target Intron 14 >>>> LeuAlaPheProCysAsnGlnPheGly{Ly} : 107 1326 bp :!!:!!||||||! |||:!!! !!.!{!:} ++MetSerPheProLeuAsnLysCysAla{Ar}- 11963 : ........................agATGTCCTTTCCCTTGAACAAGTGTGCA{AG}t : 10611 108 : >>>> Target Intron 15 >>>> {s}GlnGluProGlySerAsnGluGluIl : 116 1953 bp {!} !.!.|||||| !!:!! |||! + ++{g}TrpHisProGlyProHisProGluTh 10610 : t..........................ag{A}TGGCACCCAGGCCCCCACCCTGAGAC : 8633 117 : eLysGluPheAlaAlaGlyTyr{A} >>>> Target Intron 16 >>>> {sn : 124 !:!!!!:.!.|||:!!||| {|} 812 bp {|| rGlnAspLeuAlaSerGlyArg{A}++ ++{sn 8632 : CCAGGATCTGGCATCCGGGAGA{A}gt..........................ag{AC : 7796 125 : }ValLysPhe{A} >>>> Target Intron 17 >>>> {sp}MetPheSerLy : 132 }||| {!} 2748 bp { }! ! !||||| }ValPhePro{G}++ ++{ly}AsnSerSerLy 7795 : }GTTTTCCCA{G}gt..........................ag{GA}AATTCTTCCAA : 5025 133 : sIleCysValAsnGlyAspAspAlaHisProLeuTrpLysTrpMetLysIleGlnPro : 151 | !...!.!:::!.!! ..! ! ! |||! !|||||||||! ! !::! !! sAlaAlaAlaSerAlaValSerArgLysProTrpTrpLysTrpThrGlyAspArgThr 5024 : AGCAGCAGCATCAGCTGTGTCCAGAAAGCCATGGTGGAAATGGACAGGAGACAGGACG : 4968 152 : LysGly : 153 :!!||| GluGly 4967 : GAGGGG : 4960 vulgar: SPP00000007_1.0 4 153 . NIVO01030317.1:subseq(555646,1182752) 934285 4959 - 118 M 6 18 S 0 1 5 0 2 I 0 89830 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 13152 3 0 2 S 1 2 M 7 21 5 0 2 I 0 167662 3 0 2 M 1 3 S 0 1 5 0 2 I 0 117522 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 92579 3 0 2 S 1 2 M 9 27 S 0 2 5 0 2 I 0 14961 3 0 2 S 1 1 M 8 24 5 0 2 I 0 121917 3 0 2 M 6 18 S 0 1 5 0 2 I 0 186205 3 0 2 S 1 2 M 9 27 S 0 2 5 0 2 I 0 65929 3 0 2 S 1 1 M 2 6 5 0 2 I 0 4925 3 0 2 M 9 27 S 0 1 5 0 2 I 0 31812 3 0 2 S 1 2 M 6 18 5 0 2 I 0 8824 3 0 2 M 17 51 5 0 2 I 0 6670 3 0 2 M 1 3 5 0 2 I 0 1322 3 0 2 M 9 27 S 0 2 5 0 2 I 0 1949 3 0 2 S 1 1 M 16 48 S 0 1 5 0 2 I 0 808 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 2744 3 0 2 S 1 2 M 25 75 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 4960 934285 118 - . gene_id 19 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 934267 934285 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 934267 934285 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 934265 934266 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 844433 934266 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 844433 844434 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 844424 844432 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 844424 844432 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 844422 844423 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 831268 844423 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 831268 831269 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 831245 831267 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 831245 831267 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 831243 831244 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 663579 831244 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 663579 663580 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 663575 663578 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 663575 663578 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 663573 663574 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 663574 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 453457 546039 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 453457 453458 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 453426 453456 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 453426 453456 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 453424 453425 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 438461 453425 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 438461 438462 . - . intron_id 5 ; splice_site "aa" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 438436 438460 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 438436 438460 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 438434 438435 . - . intron_id 7 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 316515 438435 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 316515 316516 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 316496 316514 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 316496 316514 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 316494 316495 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 130287 316495 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 130287 130288 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 130256 130286 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 130256 130286 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 130254 130255 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64323 130255 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64323 64324 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64316 64322 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64316 64322 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64314 64315 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 59387 64315 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 59387 59388 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 59359 59386 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 59359 59386 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 59357 59358 . - . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 27543 59358 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 27543 27544 . - . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 27523 27542 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 27523 27542 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 27521 27522 . - . intron_id 12 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 18695 27522 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 18695 18696 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 18644 18694 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 18644 18694 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 18642 18643 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 11970 18643 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 11970 11971 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 11967 11969 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 11967 11969 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 11965 11966 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 10641 11966 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 10641 10642 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 10612 10640 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 10612 10640 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 10610 10611 . - . intron_id 15 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 8659 10611 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 8659 8660 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 8609 8658 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 8609 8658 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 8607 8608 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 7797 8608 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 7797 7798 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 7785 7796 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 7785 7796 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 7783 7784 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 5037 7784 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 5037 5038 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 4960 5036 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 4960 5036 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 4960 934285 118 - . alignment_id 19 ; Query SPP00000007_1.0 ; Align 934286 5 18 ; Align 844431 12 6 ; Align 831266 15 21 ; Align 663579 22 3 ; Align 546047 24 6 ; Align 453455 27 27 ; Align 438460 37 24 ; Align 316515 45 18 ; Align 130285 52 27 ; Align 64322 62 6 ; Align 59387 64 27 ; Align 27541 74 18 ; Align 18695 80 51 ; Align 11970 97 3 ; Align 10641 98 27 ; Align 8658 108 48 ; Align 7795 125 9 ; Align 5035 129 75 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 118 Query range: 3 -> 164 Target range: 819599 -> 36118 4 : GlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeu{A} >>>> : 20 |||!:!||| !::: ::: ::: !|||||||||! !{|} GlyLysLeuLeuGlnArgArgGluGluGluMetSerCysGlyAlaTrp{A}++ 819599 : GGGAAGCTGCTCcaaaggagagaagaagaaatgtCCTGTGGAGCTTGG{g}gt..... : 819548 21 : Target Intron 1 >>>> {la}AlaProGlyLeuAlaGlyThrMetCys >>> : 30 146065 bp {||} !||| !! !|||||| !:!:||| ++{la}TyrProArgArgAlaGlyCysLeuCys++ 819547 : ....................ag{CG}TATCCTAGGAGAGCTGGATGTCTCTGTgt... : 673454 31 : > Target Intron 2 >>>> AlaSerArgAspAspTrpArgCysAlaArgSerM : 41 86327 bp |||||||||! ! !|||||| !! !|||.!!: ++AlaSerArgGlyProTrpArgValGluArgGlyL 673453 : ......................agGCTTCCAGGGGCCCCTGGAGGGTTGAAAGGGGCT : 587096 42 : et >>>> Target Intron 3 >>>> >>>> Target Intron 4 >>>> : 42 !! 63222 bp 35193 bp eu-+ ++++ 587095 : TGct.........................aggt......................... : 523869 43 : HisGluPheSerAlaLysAspIle >>>> Target Intron 5 >>>> Asp : 50 !...|||||| !!:!!!: ! 2664 bp ||| ++GlySerPheSerLysArgGluPro++ ++Asp 523868 : agGGCAGCTTTTCCAAGAGAGAACCCgt.........................aggat : 485990 51 : GlyHisMetValAsnLeuAspLysTyrArgGlyPheVal{C} >>>> Target In : 64 ||| ::: :!! !||| ! !!||| ! |||{ } 80848 GlyLeuLeuGlyAspSerAspLeuArgTrpGlyValVal{G}++ 485989 : ggactgcttggggACAGTGACCTACGGTGGGGTGTGGTG{G}gt.............. : 485945 65 : tron 6 >>>> {ys}IleValThrAsnVal >>>> Target Intron 7 >>> : 70 bp {! } !||||||::!||| 109649 bp ++{ly}AspValThrSerVal++ 485944 : ...........ag{GA}GATGTAACATCTGTGgt........................ : 405080 71 : > AlaSerGlnUnkGlyLysThrGluValAsnTyrThrGlnLeuValAspLeuHisA : 88 !:!!||| ! ! !|||:!!:!! ! ! !...::: ||| ...| ++GlnAlaGlnValGluLeuThrGlnLeuProIleCysAspValArgAspArgGluA 405079 : .agCAGGCTCAGGTGGAGCTGACCCAGCTGCCCATCTGTGatgtgagggacagagagg : 295379 89 : laArgTyrAlaGluCysGlyLeuArgIleLeu{A} >>>> Target Intron 8 : 99 || ! !.!.!.|||..!||||||:!!! !{|} 61592 bp laThrLeuGlyHisCysSerLeuArgValSer{A}-+ 295378 : ccacCCTGGGCCACTGTTCCCTCCGTGTCTCA{G}ct..................... : 295343 100 : >>>> {la}PhePro >>>> Target Intron 9 >>>> CysAsnGln{Ph} : 105 {||}|||||| 41181 bp ||||||..!{ } ++{la}PhePro++ ++CysAsnMet{Gl} 295342 : ....ag{CA}TTCCCAgt.........................agTGCAACATG{GG} : 192555 106 : >>>> Target Intron 10 >>>> {e}GlyLysGlnGluProGlySerAsnG : 114 18834 bp { }|||......:::||| ...... ++ ++{y}GlySerSerGlnProArgAspArgT 192554 : gt..........................ag{g}ggatcttcccaacccagggatcgaa : 173694 115 : luGluIleLysGluPheAlaAla{Gl} >>>> Target Intron 11 >>>> { : 122 ::::::.........|||...{||} 109346 bp { hrGlnValSerArgIleAlaGly{Gl}+- ++{ 173693 : cccaggtctcccgtattgcaggc{gg}ga..........................ag{ : 64322 123 : y}TyrAsn >>>> Target Intron 12 >>>> ValLys >>>> Target : 127 |}|||.!. 6069 bp |||||| 110 y}TyrGln++ ++ValLys++ 64321 : C}TACCAGgt..........................agGTGAAAgt............ : 58238 128 : Intron 13 >>>> PheAspMetPheSerLysIleCysValAsnGlyAspAspAla : 140 46 bp ||| !:!:!::!!! ! :!:! :!! ! !!!:|||!.! ++PheProLeuTrpTrpAspLeu***LeuCysHisGluAspGly 58237 : ..............agTTTCCTTTATGGTGGGATCTGTAACTTTGTCATGAAGATGGC : 47155 141 : HisProLeuTrpLysTrpMetLysIleGln{Pr} >>>> Target Intron 14 : 151 ||||||||| !:::!!!:!:!!...{ } 203 bp HisProLeuThrGlyTyrLeuArgValSer{Ar}+- 47154 : CACCCTCTGACTGGTTACTTGAGGGTTTCC{AG}ga...................... : 47118 152 : >>>> {o}LysGlyLysGlyIleLeu{G} >>>> Target Intron 15 >>>> : 158 {!} !|||!..|||||||||{|} 10759 bp ++{g}GlyGlySerGlyIleLeu{G}+- 47117 : ....ag{A}GGAGGCAGTGGAATCCTG{G}gg.......................... : 46895 159 : {ly}AsnAlaIleLysTrpAsn : 164 {||} |||! !..|||||| ++{ly}ProAlaArgSerTrpAsn 46894 : ag{gc}ccAGCAAGGAGCTGGAAT : 36119 vulgar: SPP00000007_1.0 3 164 . NIVO01030317.1:subseq(555646,1182752) 819599 36118 - 118 M 16 48 S 0 1 5 0 2 I 0 146061 3 0 2 S 1 2 M 9 27 5 0 2 I 0 86323 3 0 2 M 12 36 5 0 2 I 0 63218 3 0 2 5 0 2 I 0 35189 3 0 2 M 8 24 5 0 2 I 0 2660 3 0 2 M 14 42 S 0 1 5 0 2 I 0 80844 3 0 2 S 1 2 M 5 15 5 0 2 I 0 109645 3 0 2 M 29 87 S 0 1 5 0 2 I 0 61588 3 0 2 S 1 2 M 2 6 5 0 2 I 0 41177 3 0 2 M 3 9 S 0 2 5 0 2 I 0 18830 3 0 2 S 1 1 M 16 48 S 0 2 5 0 2 I 0 109342 3 0 2 S 1 1 M 2 6 5 0 2 I 0 6065 3 0 2 M 2 6 5 0 2 I 0 11042 3 0 2 M 24 72 S 0 2 5 0 2 I 0 199 3 0 2 S 1 1 M 6 18 S 0 1 5 0 2 I 0 10755 3 0 2 S 1 2 M 6 18 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 36119 819599 118 - . gene_id 20 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 819551 819599 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 819551 819599 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 819549 819550 . - . intron_id 1 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 673486 819550 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 673486 673487 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 673457 673485 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 673457 673485 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 673455 673456 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 587130 673456 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 587130 587131 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 587094 587129 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 587094 587129 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 587092 587093 . - . intron_id 3 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 587093 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 488679 523871 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 488679 488680 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 488655 488678 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 488655 488678 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 488653 488654 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 485991 488654 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 485991 485992 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 485948 485990 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 485948 485990 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 485946 485947 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 405100 485947 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 405100 405101 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 405083 405099 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 405083 405099 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 405081 405082 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 295434 405082 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 295434 295435 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 295346 295433 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 295346 295433 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 295344 295345 . - . intron_id 8 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 233754 295345 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 233754 233755 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 233746 233753 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 233746 233753 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 233744 233745 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 192565 233745 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 192565 192566 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 192554 192564 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 192554 192564 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 192552 192553 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 173720 192553 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 173720 173721 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 173669 173719 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 173669 173719 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 173667 173668 . - . intron_id 11 ; splice_site "gA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64323 173668 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64323 64324 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64316 64322 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64316 64322 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64314 64315 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 58247 64315 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 58247 58248 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 58241 58246 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 58241 58246 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 58239 58240 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 47195 58240 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 47195 47196 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 47121 47194 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 47121 47194 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 47119 47120 . - . intron_id 14 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 46918 47120 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 46918 46919 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 46898 46917 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 46898 46917 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 46896 46897 . - . intron_id 15 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 36139 46897 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 36139 36140 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 36119 36138 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 36119 36138 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 36119 819599 118 - . alignment_id 20 ; Query SPP00000007_1.0 ; Align 819600 4 48 ; Align 673484 21 27 ; Align 587130 30 36 ; Align 488679 42 24 ; Align 485991 50 42 ; Align 405098 65 15 ; Align 295434 70 87 ; Align 233752 100 6 ; Align 192565 102 9 ; Align 173719 106 48 ; Align 64322 123 6 ; Align 58247 125 6 ; Align 47195 127 72 ; Align 46917 152 18 ; Align 36137 159 18 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 114 Query range: 1 -> 155 Target range: 606818 -> 12199 2 : SerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuAlaA : 21 ||| !||||||||| ||| !:!!:!! ! !||| ! |||::!|||:!!: SerAlaGlyArgLeuLeuArgGlyValGlnArgGlnLeuThrValGlySerLeuSerS 606818 : TCTGCTGGCCGGCTCCTGCGTGGGGTGCAGAGACAGCTAACAGTGGGGAGCTTGTCCA : 606761 22 : laPro{Gl} >>>> Target Intron 1 >>>> {y}LeuAla{Gl} >>>> : 26 :!|||{||} 162647 bp {|}||||||{||} erPro{Gl}+- ++{y}LeuAla{Gl}++ 606760 : GCCCT{GG}ga.........................ag{C}CTCGCA{GG}gt..... : 444095 27 : Target Intron 2 >>>> {y}ThrMetCysAlaSer{Ar} >>>> Target : 32 39677 bp {|}:!!:!: .!!|||{||} 901 ++{y}SerValValThrSer{Ar}++ 444094 : ....................ag{A}TCTGTCGTAACCTCC{CG}gt............ : 404400 33 : Intron 3 >>>> {g}AspAspTrpArgCys{A} >>>> Target Intron 4 : 38 49 bp {|} ||||||!!!|||{|} 13040 bp ++{g}MetAspTrpSerCys{A}++ 404399 : .............ag{G}ATGGACTGGAGTTGT{G}gt.................... : 314234 39 : >>>> {la}Arg{S} >>>> Target Intron 5 >>>> {er}MetHisGl : 43 {||}! !{|} 58238 bp {||}:!:|||.. ++{la}Pro{S}++ ++{er}ValHisSe 314233 : .....ag{CA}CCC{A}gt.........................ag{GT}GTTCACTC : 242944 44 : uPhe{Se} >>>> Target Intron 6 >>>> {r}AlaLysAspIleAspGly : 51 .|||{||} 73775 bp {|} !||||||||||||||| rPhe{Se}+- -+{r}ArgLysAspIleAspGly 242943 : CTTC{AG}gg.........................tg{C}CGGAAGGACATAGATGGG : 169145 52 : HisMetValAsnLeuAsp >>>> Target Intron 7 >>>> LysTyrArg{G : 61 ||| ! !:!!||| 6379 bp :!!|||!:!{| HisGluGlyHisLeuArg+- ++GluTyrLys{G 169144 : CATGAAGGACACCTCAGGgg.........................agGAATACAAA{G : 162736 62 : } >>>> Target Intron 8 >>>> {ly}PheValCysIleValThrAsnVal : 69 } 25112 bp {||}.!.:!!||| !:!!||| :!! }++ ++{ly}LeuLeuCysSerLeuThrValLeu 162735 : }gt.........................ag{GC}CTACTGTGCTCTCTCACTGTGCTC : 137600 70 : AlaSerGlnUnkGlyLysThrGluValAsnTyrThrGlnLeuValAspLeuHisAlaA : 89 !!!!! :::...||| ... !||| ! !:!!||| ! !|||! ProArgAlaProGluGluAlaGluLysThrThrThrThrTyrLeuAspAsnGlyAlaH 137599 : CCCAGAGccccagaggaagcagagaagaccACCACCACCTACCTGGACAACGGTGCCC : 137540 90 : rgTyrAlaGlu{C} >>>> Target Intron 9 >>>> {ys}GlyLeuArgIl : 97 .! !|||:!!{ } 98207 bp {! }!.!||||||! isProAlaGln{G}++ ++{ly}AlaLeuArgTh 137539 : ACCCTGCCCAA{G}gt.........................ag{GG}GCTCTGAGGAC : 39309 98 : eLeuAla >>>> Target Intron 10 >>>> PheProCysAsnGlnPheGly : 106 ! !||| 3383 bp ! ||||||:!!! |||||| rAlaAla++ ++ValProCysAspLeuPheGly 39308 : AGCAGCGgt..........................agGTGCCCTGTGACCTCTTTGGC : 35899 107 : LysGlnGlu{P} >>>> Target Intron 11 >>>> {ro}GlySerAsnGlu : 114 ! :!!{ } 8344 bp { !}|||......||| LeuAlaGln{G}++ ++{ly}GlyGluThrGlu 35898 : CTGGCTCAG{G}gt..........................ag{gt}ggggagactgag : 27531 115 : GluIle >>>> Target Intron 12 >>>> LysGluPheAlaAlaGlyTyrA : 124 1986 bp !||| !!! ! !!||| ! AlaGlu++ ++ValGluValGluProGlyLeuT 27530 : gcagaggt..........................agGTAGAGGTTGAACCCGGGCTCT : 25515 125 : snVal{Ly} >>>> Target Intron 13 >>>> {s}PheAspMetPheSerL : 132 :!!{!.} 2283 bp {.}......::: rpLeu{Se}++ ++{r}LeuSerLeuSerLeuP 25514 : GGCTC{AG}gt..........................ag{c}ctcagcctcagcctgC : 23208 133 : ysIleCysValAsnGlyAspAsp >>>> Target Intron 14 >>>> Ala : 141 :!!|||..! |||... ! 8325 bp ||| roLeuCysThrValGlySerLys++ ++Ala++ 23207 : CCCTCTGCACAGTGGGCTCGAAGgt..........................aggctgt : 14856 142 : >>>> Target Intron 15 >>>> HisProLeuTrpLysTrpMetLysIleGln : 150 2612 bp ||||||! !!! ...! ! :!!.!!!!. ++HisProCysCysSerSerSerGlnPheHis 14855 : ..........................agCATCCATGCTGTTCTTCAAGCCAATTTCAC : 12217 151 : ProLysGlyLysGly : 155 ||| !|||!:!||| ProGlyGlyArgGly 12216 : CCTGGGGGCAGAGGA : 12200 vulgar: SPP00000007_1.0 1 155 . NIVO01030317.1:subseq(555646,1182752) 606818 12199 - 114 M 21 63 S 0 2 5 0 2 I 0 162643 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 39673 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 90145 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 13036 3 0 2 S 1 2 M 1 3 S 0 1 5 0 2 I 0 58234 3 0 2 S 1 2 M 4 12 S 0 2 5 0 2 I 0 73771 3 0 2 S 1 1 M 12 36 5 0 2 I 0 6375 3 0 2 M 3 9 S 0 1 5 0 2 I 0 25108 3 0 2 S 1 2 M 31 93 S 0 1 5 0 2 I 0 98203 3 0 2 S 1 2 M 6 18 5 0 2 I 0 3379 3 0 2 M 10 30 S 0 1 5 0 2 I 0 8340 3 0 2 S 1 2 M 6 18 5 0 2 I 0 1982 3 0 2 M 9 27 S 0 2 5 0 2 I 0 2279 3 0 2 S 1 1 M 13 39 5 0 2 I 0 8321 3 0 2 M 1 3 5 0 2 I 0 2608 3 0 2 M 15 45 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 12200 606818 114 - . gene_id 21 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 606754 606818 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 606754 606818 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 606752 606753 . - . intron_id 1 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 444107 606753 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 444107 444108 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 444098 444106 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 444098 444106 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 444096 444097 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 404421 444097 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 404421 404422 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 404403 404420 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 404403 404420 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 404401 404402 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 314254 404402 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 314254 314255 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 314237 314253 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 314237 314253 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 314235 314236 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 301197 314236 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 301197 301198 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 301191 301196 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 301191 301196 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 301189 301190 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 242953 301190 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 242953 242954 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 242937 242952 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 242937 242952 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 242935 242936 . - . intron_id 6 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 169162 242936 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 169162 169163 . - . intron_id 5 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 169125 169161 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 169125 169161 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 169123 169124 . - . intron_id 7 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 162746 169124 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 162746 162747 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 162736 162745 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 162736 162745 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 162734 162735 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 137624 162735 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 137624 137625 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 137528 137623 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 137528 137623 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 137526 137527 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 39321 137527 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 39321 39322 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 39301 39320 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 39301 39320 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 39299 39300 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 35918 39300 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 35918 35919 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 35887 35917 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 35887 35917 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 35885 35886 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 27543 35886 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 27543 27544 . - . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 27523 27542 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 27523 27542 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 27521 27522 . - . intron_id 12 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 25537 27522 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 25537 25538 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 25508 25536 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 25508 25536 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 25506 25507 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 23225 25507 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 23225 23226 . - . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 23185 23224 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 23185 23224 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 23183 23184 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 14860 23184 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 14860 14861 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 14857 14859 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 14857 14859 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 14855 14856 . - . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 12245 14856 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 12245 12246 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 12200 12244 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 12200 12244 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 12200 606818 114 - . alignment_id 21 ; Query SPP00000007_1.0 ; Align 606819 2 63 ; Align 444106 24 6 ; Align 404420 27 15 ; Align 314253 33 15 ; Align 301195 39 3 ; Align 242951 41 12 ; Align 169161 46 36 ; Align 162746 58 9 ; Align 137622 62 93 ; Align 39319 94 18 ; Align 35918 100 30 ; Align 27541 111 18 ; Align 25537 117 27 ; Align 23224 127 39 ; Align 14860 140 3 ; Align 12245 141 45 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 114 Query range: 0 -> 163 Target range: 629611 -> 2343 1 : MetSerLeuGlyArg >>>> Target Intron 1 >>>> >>>> Target : 6 :!! !!||||||||| 105725 bp 1194 LeuArgLeuGlyArg++ ++++ 629611 : CTGCGCCTGGGCCGGgt.........................aggt............ : 523869 7 : Intron 2 >>>> LeuCysArgLeuLeuLys >>>> Target Intron 3 >> : 12 51 bp :!!|||||| !||| ! 5807 bp ++IleCysArgAsnLeuPro++ 523868 : .............agATCTGTCGTAACCTCCCGgt....................... : 404400 13 : >> ProAlaLeuLeuCysGlyAlaLeuAlaAlaPro{Gl} >>>> Target Int : 23 !! ! !|||||| !:!! !:!!||||||{.!} 5175 b ++ThrLeuAsnLeuCysHisSerSerSerAlaPro{Se}-+ 404399 : ..agACACTGAATCTGTGTCATTCAAGCTCTGCTCCC{AG}tt............... : 398558 24 : ron 4 >>>> {y}LeuAlaGlyThrMetCysAlaSerArgAspAspTrpArgCysA : 38 p {!}|||!.!|||:!! ||||||!!! ...|||||| : ++{r}LeuValGlySerTyrCysAlaThrGlyLeuSerTrpArgLeuS 398557 : ..........ag{T}CTAGTTGGTTCATATTGTGCTACTggactttcctggcggctca : 393342 39 : la{Ar} >>>> Target Intron 5 >>>> {g}SerMetHisGluPheSerAl : 46 ::{ !} 113451 bp {!}:::! !|||! !.!.! er{Tr}++ ++{p}AlaAsnSerGluSerLysTh 393341 : gc{tg}gt.........................ag{g}gcgAACAGTGAATCAAAAAC : 279867 47 : a{L} >>>> Target Intron 6 >>>> {ys}AspIleAspGlyHisMetVal : 54 !{:} 33633 bp {!!}|||||| !||| ! ! r{G}++ ++{lu}AspIleLeuGlyIleArgHis 279866 : G{G}gt.........................ag{AG}GACATACTTGGCATCCGTCAT : 246210 55 : AsnLeuAspLys{T} >>>> Target Intron 7 >>>> {yr}ArgGlyPheV : 63 :!!||||||..!{!} 19349 bp {:!}!!!!.! !: AspLeuAspSer{P}++ ++{he}SerAlaGlyL 246209 : GATTTAGACTCA{T}gt.........................ag{TT}AGTGCTGGCC : 226834 64 : alCysIleValThrAsnValAlaSerGln{U} >>>> Target Intron 8 >>> : 73 !!! :!!|||||||||!.!:!!.!!|||{ } 130307 bp euLeuValValThrAsnAlaSerGlyGln{G}++ 226833 : TGTTGGTTGTGACCAACGCTTCGGGTCAA{G}gt........................ : 226801 74 : > {nk}GlyLysThrGlu >>>> Target Intron 9 >>>> ValAsnTyrT : 81 { }|||:::|||||| 1092 bp |||!!.!:!| ++{ly}GlyGluThrGlu+- ++ValLysPheT 226800 : .ag{gt}ggagaaactgaggc.........................agGTGAAATTCA : 95381 82 : hrGlnLeuValAspLeuHisAlaArgTyrAlaGluCys >>>> Target Intron : 94 ||.!.:!!!.!..! !! ||| ! |||!!: 23138 bp hrAspValAlaSerSerProAlaTyrValAlaAspGln++ 95380 : CAGATGTGGCCTCTAGTCCAGCATATGTGGCAGATCAGgt.................. : 95340 95 : 10 >>>> {G} >>>> Target Intron 11 >>>> {ly}LeuArgIleLe : 98 {|} 6503 bp {||}|||!:!!!:! ++{G}++ ++{ly}LeuLysMetGl 95339 : ........ag{G}gt..........................ag{GC}CTGAAGATGCA : 65689 99 : uAlaPhePro >>>> Target Intron 12 >>>> CysAsnGlnPheGlyLys : 107 !!.!.!!||| 6291 bp ||| ...||| nGlyLeuPro++ ++CysValValIleGlyLeu 65688 : GGGGCTCCCAgt..........................agTGTGTGGTCatcgggctg : 59371 108 : GlnGluPro{G} >>>> Target Intron 13 >>>> {ly}SerAsnGluGlu : 115 |||... {|} 1922 bp {||}|||.!.:!!||| GlnSerThr{G}++ ++{ly}SerGluGlnGlu 59370 : cagtccaca{g}gt..........................ag{GC}TCTGAGCAGGAA : 57425 116 : Ile{Ly} >>>> Target Intron 14 >>>> {s}GluPheAlaAlaGlyTyr : 123 :!!{!:} 8844 bp {!}:!!!:!|||:!!! !||| Val{Ar}+- ++{g}LysTyrAlaSerValTyr 57424 : GTC{AG}gg..........................ag{A}AAATATGCCTCAGTTTAC : 48557 124 : AsnValLysPheAspMetPheSer{Ly} >>>> Target Intron 15 >>>> : 132 ..!:::... ...|||...{! } 10233 bp ***ThrArgIleCysPhePheGlu{Me}++ ++ 48556 : TGAACACgtatttgtttctttgaa{at}gt..........................ag : 38297 133 : {s}IleCysVal{A} >>>> Target Intron 16 >>>> {sn}GlyAsp{A} : 139 {!} !|||! !{ } 7544 bp { !}|||!!:{|} {t}CysCysGly{A}++ ++{la}GlyGlu{A} 38296 : {G}TGCTGTGGT{G}gt..........................ag{CT}GGGGAA{G} : 30732 140 : >>>> Target Intron 17 >>>> {sp}AlaHisProLeuTrpLysTrpMet : 147 3463 bp {||}::!||||||:!! !!:!! :!! ++ ++{sp}SerHisProValArgGlnAlaLeu 30731 : gt..........................ag{AC}AGCCACCCTGTGAGGCAGGCCCTG : 27245 148 : LysIleGlnProLysGly{Ly} >>>> Target Intron 18 >>>> {s}Gly : 155 ! !! ||| {!:} 2770 bp {!} ! LeuSerProProPheArg{Ar}++ ++{g}Gln 27244 : TTATCACCTCCATTTaga{ag}gt..........................ag{G}CAG : 24451 156 : IleLeuGly{As} >>>> Target Intron 19 >>>> {n} >>>> Targe : 160 :::||||||{..} 6831 bp {.} 1 LeuLeuGly{Gl}++ ++{y}++ 24450 : Ctcctggga{gg}gt..........................ag{G}gt.......... : 17603 161 : t Intron 20 >>>> AlaIleLysTrp : 163 5250 bp ::!:!!!!.||| ++SerLeuAsnTrp 17602 : ................agAGTCTCAACTGG : 2344 vulgar: SPP00000007_1.0 0 163 . NIVO01030317.1:subseq(555646,1182752) 629611 2343 - 114 M 5 15 5 0 2 I 0 105721 3 0 2 5 0 2 I 0 119447 3 0 2 M 6 18 5 0 2 I 0 5803 3 0 2 M 11 33 S 0 2 5 0 2 I 0 5171 3 0 2 S 1 1 M 15 45 S 0 2 5 0 2 I 0 113447 3 0 2 S 1 1 M 7 21 S 0 1 5 0 2 I 0 33629 3 0 2 S 1 2 M 11 33 S 0 1 5 0 2 I 0 19345 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 130303 3 0 2 S 1 2 M 4 12 5 0 2 I 0 1088 3 0 2 M 16 48 5 0 2 I 0 23134 3 0 2 S 0 1 5 0 2 I 0 6499 3 0 2 S 1 2 M 7 21 5 0 2 I 0 6287 3 0 2 M 9 27 S 0 1 5 0 2 I 0 1918 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 8840 3 0 2 S 1 1 M 14 42 S 0 2 5 0 2 I 0 10229 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 7540 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 3459 3 0 2 S 1 2 M 14 42 S 0 2 5 0 2 I 0 2766 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 6827 3 0 2 S 1 1 5 0 2 I 0 15246 3 0 2 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 2344 629611 114 - . gene_id 22 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 629597 629611 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 629597 629611 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 629595 629596 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 629596 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 404421 523871 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 404421 404422 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 404403 404420 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 404403 404420 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 404401 404402 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 398596 404402 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 398596 398597 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 398561 398595 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 398561 398595 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 398559 398560 . - . intron_id 4 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 393386 398560 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 393386 393387 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 393338 393385 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 393338 393385 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 393336 393337 . - . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 279887 393337 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 279887 279888 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 279864 279886 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 279864 279886 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 279862 279863 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 246231 279863 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 246231 246232 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 246195 246230 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 246195 246230 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 246193 246194 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 226846 246194 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 226846 226847 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 226804 226845 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 226804 226845 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 226802 226803 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 96497 226803 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 96497 96498 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 96483 96496 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 96483 96496 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 96481 96482 . - . intron_id 9 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 95391 96482 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 95391 95392 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 95343 95390 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 95343 95390 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 95341 95342 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 72205 95342 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 72205 72206 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 72204 72204 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 72204 72204 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 72202 72203 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 65701 72203 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 65701 65702 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 65678 65700 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 65678 65700 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 65676 65677 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 59387 65677 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 59387 59388 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 59359 59386 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 59359 59386 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 59357 59358 . - . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 57437 59358 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 57437 57438 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 57418 57436 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 57418 57436 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 57416 57417 . - . intron_id 14 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 48574 57417 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 48574 48575 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 48529 48573 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 48529 48573 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 48527 48528 . - . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 38296 48528 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 38296 38297 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 38285 38295 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 38285 38295 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 38283 38284 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 30741 38284 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 30741 30742 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 30732 30740 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 30732 30740 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 30730 30731 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 27269 30731 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 27269 27270 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 27223 27268 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 27223 27268 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 27221 27222 . - . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 24453 27222 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 24453 24454 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 24438 24452 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 24438 24452 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 24436 24437 . - . intron_id 19 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 17607 24437 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 17607 17608 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 17606 17606 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 17606 17606 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 17604 17605 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 2356 17605 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 2356 2357 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 2344 2355 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 2344 2355 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 2344 629611 114 - . alignment_id 22 ; Query SPP00000007_1.0 ; Align 629612 1 15 ; Align 404421 6 18 ; Align 398596 12 33 ; Align 393385 24 45 ; Align 279886 40 21 ; Align 246229 48 33 ; Align 226844 60 39 ; Align 96495 74 12 ; Align 95391 78 48 ; Align 65699 95 21 ; Align 59387 102 27 ; Align 57435 112 15 ; Align 48573 118 42 ; Align 38295 133 9 ; Align 30739 137 6 ; Align 27267 140 42 ; Align 24452 155 12 ; Align 2356 160 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 113 Query range: 0 -> 196 Target range: 1066744 -> 55865 1 : MetSerLeu{G} >>>> Target Intron 1 >>>> {ly}ArgLeu{C} : 7 ||||||:::{|} 50145 bp {||} !:!!{ } MetSerMet{G}++ ++{ly}AlaVal{G}++ 1066744 : atgtccatg{g}gt.........................ag{GC}GCTGTA{G}gt : 1016580 8 : >>>> Target Intron 2 >>>> {ys}ArgLeuLeuLysProAlaLeuLeuC : 16 60146 bp {! } ||| |||... ! ++{ly}AspLeuProAspProGlyAlaLysT 1016579 : .........................ag{gg}gaccttcccgacccaggggccaAAA : 956408 17 : ysGlyAlaLeuAlaAlaProGlyLeuAlaGlyThrMetCysAlaSerArgAspAsp : 34 !|||...|||...:::|||...||||||||| ... ... hrGlyCysLeuValSerProAlaLeuAlaGlyArgPhePheThrProSerAlaAla 956407 : CTgggtgtcttgtgtctcctgcgttggcaggcaggttcttcacccctagcgccgcc : 956354 35 : {Tr} >>>> Target Intron 3 >>>> {p}Arg{C} >>>> Target : 37 {||} 800 bp {|}..!{|} 616 {Tr}+- ++{p}Glu{C}++ 956353 : {tg}gg.........................ag{G}GAA{T}gt............ : 955542 38 : Intron 4 >>>> {ys}AlaArgSerMetHisGluPheSerAlaLysAspIleA : 50 54 bp {||} !!!!! !! !!!.!!: !..!:!!!:!! !! !. ++{ys}LeuSerTyrArgGlnAspProGluSerArgGlyThrS 955541 : .............ag{GC}CTGAGCTATAGGCAGGACCCTGAGTCCAGGGGCACAT : 893852 51 : spGlyHisMetValAsnLeu{As} >>>> Target Intron 5 >>>> {p} : 57 ....!|||..!:::...:::{! } 41114 bp {!} erAsnHisGlnLeuArgVal{Gl}++ ++{y} 893851 : CGAACCATCAGCtgagggtg{gg}gt.........................ag{C} : 852715 58 : LysTyrArgGlyPheValCysIleValThrAsnValAlaSerGlnUnkGlyLysTh : 76 ||| !!:!||| ||| ! !:!!! !...:!!||||||:::...... || LysSerLysGlyLysValGlnArgLeuArgGlyIleAlaSerGluThrAsnThrTh 852714 : AAAAGTAAGGGGAAGGTGCAGAGATTAAGAGGGATTGcttcagaaaccaacacaac : 852660 77 : rGluVal{A} >>>> Target Intron 6 >>>> {sn}TyrThrGlnLeuV : 84 | {|} 31923 bp {||}|||!:!||| !| rLeuTyr{A}+- ++{sn}TyrSerGlnThrV 852659 : attgtac{a}gc.........................ag{AC}TACAGCCAGACTG : 820713 85 : alAspLeuHisAlaArgTyrAlaGluCysGlyLeu{A} >>>> Target Intr : 96 || ! !! ! !! !|||...||||||{|} 22692 b alThrSerProLysGlyThrLysGluAlaGlyLeu{A}++ 820712 : TTACATCTCCAAAGGGAACGAAGGAGGCAGGCCTA{C}gt................ : 820674 97 : on 7 >>>> {rg}{I} >>>> Target Intron 8 >>>> {le}LeuAl : 99 p {||}{:} 68166 bp {!!}||||| ++{rg}{V}+- -+{al}LeuAl 820673 : .........ag{ga}{g}ga.........................cg{TC}TTGGC : 729810 100 : aPheProCysAsnGlnPheGlyLysGlnGluProGlySerAsnGluGluIleLys{ : 118 ||||||| ! ..! !! !||| !|||! !||||||!.!::!! !:!!{ aPheProArgGlyValSerGlyValGlnGlyProGlyLysSerAlaPheArgGlu{ 729809 : TTTTCCAAGAGGTGTGTCTGGGGTGCAGGGACCTGGAAAGTCCGCTTTTCGTGAA{ : 729753 119 : Gl} >>>> Target Intron 9 >>>> {u}PheAla{A} >>>> Targe : 121 } 115 bp { }||||||{|} 1 Cy}++ ++{s}PheAla{A}++ 729752 : TG}gt.........................ag{C}TTTGCA{G}gt.......... : 729626 122 : t Intron 10 >>>> {la}Gly{Ty} >>>> Target Intron 11 >>> : 123 1226 bp {||}|||{ } 40941 bp ++{la}Gly{Ar}++ 729625 : ................ag{CA}GGA{AG}gt......................... : 718393 124 : > {r}AsnValLysPheAspMetPheSerLysIleCys{V} >>>> Target : 135 { }.!.:!!! ||| !!...||| !!:!!||| {|} 698 ++{g}GlnIleThrPheHisGlnPheProGlnIleSer{V}++ 718392 : .ag{A}CAAATTACTTTTCACCAATTTCCCCAGatatct{g}gt............ : 677417 136 : Intron 12 >>>> {al}AsnGlyAspAspAlaHisProLeuTrpLys{T} > : 146 97 bp {||}:::|||||| ...... :::||| {|} ++{al}SerGlyAspLeuGlyArgTrpValTrpAla{T}++ 677416 : ..............ag{TT}TCAGGcgatttggggaggtgggtttgggca{t}gt. : 607487 147 : >>> Target Intron 13 >>>> {rp}MetLysIleGlnProLysGly{L} : 154 118811 bp {||}..! .!!||| !!:! !{ } ++{rp}GlnLeuPheGlnGluArgThr{H}+ 607486 : .........................ag{GG}CAGCTTTTCCAAGAGAGAACC{C}g : 488654 155 : >>>> Target Intron 14 >>>> {ys}GlyIleLeuGlyAsn{A} >>> : 160 11698 bp {! } !! |||||||||{ } + ++{is}ArgGluLeuGlyAsn{P}++ 488653 : t..........................ag{AC}AGGGAGCTGGGGAAT{C}gt... : 476936 161 : > Target Intron 15 >>>> {la}IleLysTrpAsn{P} >>>> Targe : 165 175626 bp {!!}! !! |||!!.{ } 1 ++{ro}AsnThrTrpLys{G}++ 476935 : .......................ag{CC}AACACCTGGAAA{G}gt.......... : 301295 166 : t Intron 16 >>>> {he}ThrLysPheLeuIleAspLysAsnGlyCysValV : 177 50202 bp { }||| !||||||:!!..!! ...||||||!.! ++{ly}ThrGlyPheLeuValSerThrGlyGlyCysAlaC 301294 : ................ag{GG}ACAGGGTTCCTCGTCAGTACTGGAGGGTGTGCTT : 151060 178 : al{Ly} >>>> Target Intron 17 >>>> {s}ArgTyrGlyProMetGl : 184 !{!:} 95135 bp {!}! ! !! !|||:!::! ys{Ar}+- ++{g}ProAspProProLeuGl 151059 : GC{AG}gg..........................ag{G}CCGGACCCACCGCTCCA : 55904 185 : uGluProLeuValIleGluLysAspLeuProHisTyr : 196 ! ! !||| ! !! ! ! !!|||||||||!:! nProGlyLeuCysAlaGlyValHisLeuProHisPhe 55903 : ACCAGGTCTCTGCGCCGGAGTACATCTTCCTCATTTC : 55866 vulgar: SPP00000007_1.0 0 196 . NIVO01030317.1:subseq(555646,1182752) 1066744 55865 - 113 M 3 9 S 0 1 5 0 2 I 0 50141 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 60142 3 0 2 S 1 2 M 27 81 S 0 2 5 0 2 I 0 796 3 0 2 S 1 1 M 1 3 S 0 1 5 0 2 I 0 61650 3 0 2 S 1 2 M 19 57 S 0 2 5 0 2 I 0 41110 3 0 2 S 1 1 M 21 63 S 0 1 5 0 2 I 0 31919 3 0 2 S 1 2 M 16 48 S 0 1 5 0 2 I 0 22688 3 0 2 S 1 3 5 0 2 I 0 68162 3 0 2 S 1 2 M 20 60 S 0 2 5 0 2 I 0 111 3 0 2 S 1 1 M 2 6 S 0 1 5 0 2 I 0 11222 3 0 2 S 1 2 M 1 3 S 0 2 5 0 2 I 0 40937 3 0 2 S 1 1 M 11 33 S 0 1 5 0 2 I 0 69893 3 0 2 S 1 2 M 10 30 S 0 1 5 0 2 I 0 118807 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 11694 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 175622 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 150198 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 95131 3 0 2 S 1 1 M 18 54 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 55866 1066744 113 - . gene_id 23 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1066735 1066744 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1066735 1066744 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1066733 1066734 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016590 1066734 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016590 1016591 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016589 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016589 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 956435 1016580 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 956435 956436 . - . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 956350 956434 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 956350 956434 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 956348 956349 . - . intron_id 3 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 955550 956349 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 955550 955551 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 955545 955549 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 955545 955549 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 955543 955544 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 893891 955544 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 893891 893892 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 893830 893890 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 893830 893890 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 893828 893829 . - . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 852716 893829 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 852716 852717 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 852651 852715 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 852651 852715 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 852649 852650 . - . intron_id 6 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 820728 852650 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 820728 820729 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 820677 820727 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 820677 820727 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 820675 820676 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 797985 820676 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 797985 797986 . - . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 797982 797984 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 797982 797984 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 797980 797981 . - . intron_id 8 ; splice_site "ga" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 729816 797981 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 729816 729817 . - . intron_id 7 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 729752 729815 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 729752 729815 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 729750 729751 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 729637 729751 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 729637 729638 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 729629 729636 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 729629 729636 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 729627 729628 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 718403 729628 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 718403 718404 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 718396 718402 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 718396 718402 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 718394 718395 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 677455 718395 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 677455 677456 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 677420 677454 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 677420 677454 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 677418 677419 . - . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 607523 677419 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 607523 607524 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 607490 607522 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 607490 607522 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 607488 607489 . - . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 488679 607489 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 488679 488680 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 488655 488678 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 488655 488678 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 488653 488654 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 476957 488654 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 476957 476958 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 476939 476956 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 476939 476956 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 476937 476938 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 301313 476938 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 301313 301314 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 301298 301312 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 301298 301312 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 301296 301297 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 151096 301297 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 151096 151097 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 151056 151095 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 151056 151095 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 151054 151055 . - . intron_id 17 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 55921 151055 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 55921 55922 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 55866 55920 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 55866 55920 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 55866 1066744 113 - . alignment_id 23 ; Query SPP00000007_1.0 ; Align 1066745 1 9 ; Align 1016588 5 6 ; Align 956433 8 81 ; Align 955549 36 3 ; Align 893889 38 57 ; Align 852715 58 63 ; Align 820726 80 48 ; Align 729814 98 60 ; Align 729636 119 6 ; Align 718401 122 3 ; Align 677454 124 33 ; Align 607521 136 30 ; Align 488677 147 21 ; Align 476955 155 15 ; Align 301311 161 12 ; Align 151094 166 36 ; Align 55920 179 54 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 114 Query range: 1 -> 167 Target range: 1008876 -> 24247 2 : SerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCysGlyAlaLeuAl : 20 |||! !||| ! !||| !! !:!!:!!|||!.! !:!! !|||...|||:: SerGlnGlySerThrCysGlyThrValGluProValThrValHisGlyThrLeuSe 1008876 : AGTCAGGGCTCGACCTGTGGGACAGTTGAGCCCGTAACGGTACACGGaactctctc : 1008822 21 : aAlaProGlyLeuAlaGlyThrMetCysAlaSer >>>> Target Intron 1 : 32 ::::|||... |||! !..!.!.! !|||! ! 32028 bp rSerProSerSerAlaValValPheSerAlaMet++ 1008821 : ttctccctcttcagCTGTGGTTTTCTCTGCGATGgt.................... : 1008784 33 : >>>> >>>> Target Intron 2 >>>> ArgAspAspTrpArgCysAl : 38 92368 bp !:!|||||| !! !! ! ++++ ++LysAspAspGluLeuSerAr 1008783 : .....aggt.........................agAAGGACGATGAGCTTTCCAG : 884372 39 : aArgSer >>>> Target Intron 3 >>>> MetHisGlu >>>> Targ : 44 !|||!!! 39931 bp ! |||:!! 1 gArgThr++ ++AsnHisGln++ 884371 : AAGGACGgt.........................agAACCACCAGgt......... : 844421 45 : et Intron 4 >>>> PheSer{A} >>>> Target Intron 5 >>>> : 46 16286 bp !|||{!} 127550 bp ++AspSer{G}++ ++ 844420 : ................agGATTCA{G}gt.........................ag : 600582 47 : {la}LysAsp >>>> Target Intron 6 >>>> >>>> Target Int : 49 {.!}|||!!: 76701 bp 11419 {ly}LysGlu++ ++++ 600581 : {GA}AAGGAAgt.........................aggt............... : 523869 50 : ron 7 >>>> IleAspGlyHisMetValAsnLeuAspLysTyr{A} >>>> T : 60 bp :::... !||| !! !||||||... {|} ++ValSerTrpHisHisPheLeuLeuAspSerThr{A}++ 523868 : ..........aggtaagttgGCATCATTTCCTTTTAGACTCCACA{C}gt...... : 512416 61 : arget Intron 8 >>>> {rg}Gly >>>> Target Intron 9 >>>> : 62 110759 bp {||}||| 74625 bp ++{rg}Gly++ + 512415 : ...................ag{GT}GGGgt.........................a : 327031 63 : PheValCysIleValThrAsnValAlaSerGlnUnk{G} >>>> Target In : 74 ||| !|||:!! !! !!|||! ! !|||||| {|} 18525 +PheSerCysValPheProAsnGluPheSerGln***{G}++ 327030 : gTTTTCCTGTGTCTTCCCAAATGAGTTTAGTCAATAG{G}gt.............. : 326990 75 : tron 10 >>>> {ly}LysThrGlu >>>> Target Intron 11 >>>> : 78 bp {||}||||||! 61412 bp ++{ly}LysThrAla++ + 326989 : ............ag{GG}AAAACGGCCgt..........................a : 247046 79 : ValAsnTyrThrGlnLeuValAspLeuHis >>>> Target Intron 12 > : 88 |||!.! ||| !|||:!!..! !.!. 37714 bp +ValThrValThrThrLeuIleSerLysGlu++ 247045 : gGTTACTGTGACCACACTGATAAGCAAAGAGgt....................... : 247012 89 : >>> AlaArgTyrAlaGluCys{Gl} >>>> Target Intron 13 >>>> : 94 !.!|||!::::! !|||{.!} 1140 bp ++GlyArgTrpSerTrpCys{Se}++ + 247011 : ...agGGCAGGTGGAGCTGGTGC{AG}gt..........................a : 208142 95 : {y}LeuArgIleLeuAlaPheProCysAsnGlnPheGly{Ly} >>>> Targe : 107 {!}|||||| ! !::!.!!||| ::!! !! !{!:} 2 +{r}LeuArgAlaLysSerLeuProGlnSerLeuValThr{Ar}++ 208141 : g{T}CTGAGAGCAAAGAGTCTCCCACAGTCTCTTGTTACA{AG}gt.......... : 208099 108 : t Intron 14 >>>> {s}GlnGluProGlySerAsnGluGluIleLysGluPh : 119 2192 bp {!}.!. |||||| ...||| ::::::||| ++{g}AspProProGlyLeuGluGluGlyValGluGluGl 208098 : ................ag{G}GACCCacctgggctggaggagggggtggaggagcA : 185875 120 : eAlaAlaGlyTyr >>>> Target Intron 15 >>>> AsnValLysPheA : 128 :!!!.!|||:!! 56518 bp |||:!!! ||| nSerGlyGlyHis+- ++AsnLeuThrPheL 185874 : GTCAGGTGGGCACgg..........................agaACCTGACCTTCA : 129330 129 : sp >>>> Target Intron 16 >>>> MetPheSerLysIleCysValAsn : 136 ! 185 bp :!!! :!! !:!!|||..!.!. ys+- ++Leu***AlaGlyLeuCysThrGln 129329 : AGgg..........................agCTGTGAGCAGGGCTCTGCACTCAG : 129121 137 : >>>> Target Intron 17 >>>> GlyAspAsp >>>> Target Int : 140 4346 bp ||| ||| 32808 ++ ++GlyTrpAsp++ 129120 : gt..........................agggaTGGGATgt............... : 124761 141 : ron 18 >>>> AlaHisProLeu >>>> Target Intron 19 >>>> T : 144 bp |||!!.|||||| 39506 bp | ++AlaGlnProLeu++ ++T 124760 : ...........agGCCCAGCCCCTGgt..........................agT : 52437 145 : rpLysTrpMetLys >>>> Target Intron 20 >>>> >>>> Targe : 149 || ! :!! 10474 bp rpGlyValGlyGlu++ ++++ 52436 : GGGGTGTGGGTGAGgt..........................aggt.......... : 41946 150 : t Intron 21 >>>> IleGlnPro{Ly} >>>> Target Intron 22 > : 152 809 bp ! ...|||{!:} 12328 bp ++ArgSerPro{Ar}++ 41945 : ................agAGGAGCCCA{AG}gt....................... : 41126 153 : >>> {s}GlyLysGly >>>> Target Intron 23 >>>> IleLeuGly : 158 {!}|||!:!||| 4507 bp :!:||| ! ++{g}GlyArgGly++ +-LeuLeuPro 41125 : ...ag{G}GGAAGGGGTgt..........................acCTGTTACCC : 24277 159 : AsnAlaIleLysTrpAsnPheThrLys : 167 |||!.!:!!...|||!:!||| !||| AsnValLeuSerTrpSerPheHisLys 24276 : AACGTCCTCTCCTGGAGCTTTCACAAA : 24248 vulgar: SPP00000007_1.0 1 167 . NIVO01030317.1:subseq(555646,1182752) 1008876 24247 - 114 M 30 90 5 0 2 I 0 32024 3 0 2 5 0 2 I 0 92364 3 0 2 M 9 27 5 0 2 I 0 39927 3 0 2 M 3 9 5 0 2 I 0 116282 3 0 2 M 2 6 S 0 1 5 0 2 I 0 127546 3 0 2 S 1 2 M 2 6 5 0 2 I 0 76697 3 0 2 5 0 2 I 0 11415 3 0 2 M 11 33 S 0 1 5 0 2 I 0 110755 3 0 2 S 1 2 M 1 3 5 0 2 I 0 74621 3 0 2 M 12 36 S 0 1 5 0 2 I 0 18521 3 0 2 S 1 2 M 3 9 5 0 2 I 0 61408 3 0 2 M 10 30 5 0 2 I 0 37710 3 0 2 M 6 18 S 0 2 5 0 2 I 0 1136 3 0 2 S 1 1 M 12 36 S 0 2 5 0 2 I 0 22188 3 0 2 S 1 1 M 16 48 5 0 2 I 0 56514 3 0 2 M 5 15 5 0 2 I 0 181 3 0 2 M 8 24 5 0 2 I 0 4342 3 0 2 M 3 9 5 0 2 I 0 32804 3 0 2 M 4 12 5 0 2 I 0 39502 3 0 2 M 5 15 5 0 2 I 0 10470 3 0 2 5 0 2 I 0 805 3 0 2 M 3 9 S 0 2 5 0 2 I 0 12324 3 0 2 S 1 1 M 3 9 5 0 2 I 0 4503 3 0 2 M 12 36 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 24248 1008876 114 - . gene_id 24 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1008787 1008876 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1008787 1008876 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1008785 1008786 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1008786 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 884391 976758 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 884391 884392 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 884364 884390 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 884364 884390 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 884362 884363 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 844433 884363 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 844433 844434 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 844424 844432 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 844424 844432 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 844422 844423 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 728138 844423 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 728138 728139 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 728131 728137 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 728131 728137 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 728129 728130 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 600581 728130 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 600581 600582 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 600573 600580 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 600573 600580 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 600571 600572 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 600572 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 512453 523871 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 512453 512454 . - . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 512419 512452 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 512419 512452 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 512417 512418 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 401660 512418 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 401660 401661 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 401655 401659 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 401655 401659 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 401653 401654 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 327030 401654 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 327030 327031 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 326993 327029 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 326993 327029 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 326991 326992 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 308468 326992 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 308468 308469 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 308457 308467 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 308457 308467 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 308455 308456 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247045 308456 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247045 247046 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247015 247044 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247015 247044 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247013 247014 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 209301 247014 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 209301 209302 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 209281 209300 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 209281 209300 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 209279 209280 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 208141 209280 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 208141 208142 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 208102 208140 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 208102 208140 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 208100 208101 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 185910 208101 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 185910 185911 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 185861 185909 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 185861 185909 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 185859 185860 . - . intron_id 15 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 129343 185860 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 129343 129344 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 129328 129342 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 129328 129342 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 129326 129327 . - . intron_id 16 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 129143 129327 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 129143 129144 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 129119 129142 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 129119 129142 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 129117 129118 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 124773 129118 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 124773 124774 . - . intron_id 16 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 124764 124772 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 124764 124772 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 124762 124763 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 91956 124763 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 91956 91957 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 91944 91955 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 91944 91955 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 91942 91943 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 52438 91943 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 52438 52439 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 52423 52437 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 52423 52437 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 52421 52422 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 52422 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41140 41948 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41140 41141 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 41129 41139 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 41129 41139 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41127 41128 . - . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 28801 41128 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 28801 28802 . - . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 28791 28800 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 28791 28800 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 28789 28790 . - . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 24284 28790 . - . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 24284 24285 . - . intron_id 22 ; splice_site "AC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 24248 24283 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 24248 24283 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 24248 1008876 114 - . alignment_id 24 ; Query SPP00000007_1.0 ; Align 1008877 2 90 ; Align 884391 32 27 ; Align 844433 41 9 ; Align 728138 44 6 ; Align 600579 47 6 ; Align 512453 49 33 ; Align 401658 61 3 ; Align 327030 62 36 ; Align 308466 75 9 ; Align 247045 78 30 ; Align 209301 88 18 ; Align 208140 95 36 ; Align 185909 108 48 ; Align 129343 124 15 ; Align 129143 129 24 ; Align 124773 137 9 ; Align 91956 140 12 ; Align 52438 144 15 ; Align 41140 149 9 ; Align 28800 153 9 ; Align 24284 156 36 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 109 Query range: 59 -> 194 Target range: 1167780 -> 297854 60 : ArgGlyPheValCysIleValThrAsnValAlaSerGln{Un} >>>> Target : 73 ...||||||:!!||| ! ! !|||:!!.!!:!!:!!{ } 43 GluGlyPheIleCysAlaArgGlyAsnIleThrAlaGlu{Ar}++ 1167780 : gagggcTTTATTTGTGCAAGGGGCAATATTACTGCAGAG{AG}gt........... : 1167737 74 : Intron 1 >>>> {k}GlyLys{Th} >>>> Target Intron 2 >>>> : 76 004 bp { }|||!:!{||} 61167 bp ++{g}GlyArg{Th}++ 1167736 : ..............ag{G}GGCAGG{AC}gt......................... : 1124724 77 : {r}GluValAsnTyrThrGlnLeuValAsp{Le} >>>> Target Intron : 86 {|}::: |||:::...... ::: { } 24341 bp ++{r}AspArgAsnTrpValSerCysIleGly{Ar}++ 1124723 : ag{g}gatcgaaactgggtctcctgtattggc{ag}gt.................. : 1063527 87 : 3 >>>> {u}HisAlaArgTyrAlaGluCysGlyLeuArgIleLeuAlaPhePr : 101 {!}||| ::: ... !:!!!:! !|||..!||| ++{g}HisLeuSerPheLeuSerGluThrMetLysSerLeuCysPheAr 1063526 : .......ag{g}catttgtcttttctctctgaaacTATGAAATCTCTTTGTTTTAG : 1039145 102 : oCysAsnGlnPhe >>>> Target Intron 4 >>>> >>>> Target : 106 !||| !... ! 62372 bp 534 gCysValSerHis++ ++++ 1039144 : GTGTGTTTCCCACgt.........................aggt............ : 976756 107 : Intron 5 >>>> GlyLysGlnGluPro{G} >>>> Target Intron 6 : 111 64 bp |||::!! !! !|||{|} 31189 bp ++GlyArgLeuGlyPro{G}++ 976755 : .............agGGGCGGCTGGGGCCA{G}gt..................... : 923276 112 : >>>> {ly}SerAsnGluGluIleLysGluPheAlaAlaGlyTyr{A} >>>> : 124 {||}||||||:!!||| ::: ! ! !!||| ! {.} ++{ly}SerAsnLysGluAlaArgLeuThrArgProGlyGln{G}++ 923275 : ....ag{GT}AGCAATAAGGAAGCGCGTTTGACACGGCCCGGCCAA{G}gt..... : 892048 125 : Target Intron 7 >>>> {sn}ValLysPheAspMetPheSerLysIleCys : 134 64567 bp {..}!.! ||| ::: ||||||||| ++{ly}AlaValPheCysLeuGlySerLysIleThr 892047 : ....................ag{GG}GCTGTtttttgcttgggctccaaaatcact : 827454 135 : ValAsn{G} >>>> Target Intron 8 >>>> {ly}{A} >>>> Targ : 138 ...:::{|} 29460 bp {||}{|} AlaAsp{G}++ ++{ly}{A}+- 827453 : gcagac{g}gt.........................ag{ga}{g}ga......... : 797979 139 : et Intron 9 >>>> {sp}AspAlaHisProLeuTrpLys >>>> Target : 146 70630 bp {||}|||:!! ! !||| !:! 25 ++{sp}AspSerLysMetLeuAlaArg++ 797978 : ................ag{AC}GATTCCAAAATGCTGGCTAGAgt........... : 727326 147 : Intron 10 >>>> TrpMet{L} >>>> Target Intron 11 >>>> : 148 447 bp ||| { } 15346 bp ++TrpSer{C}++ ++ 727325 : ...............agtggtca{t}gt..........................ag : 686530 149 : {ys}IleGlnProLysGlyLysGlyIleLeu >>>> Target Intron 12 > : 158 { }|||:::|||:::... ...::: 29212 bp {ys}IleGluProArgSerProAlaLeuGln++ 686529 : {gc}atcgaacccaggtctcctgcattgcaggt....................... : 686497 159 : >>> GlyAsnAlaIleLysTrpAsnPheThr >>>> Target Intron 13 : 167 |||||| !||| ! ||| !|||! ! 133389 bp +-GlyAsnTyrIleTyrTrpCysPheMet++ 686496 : ...aaGGCAATTACATATATTGGTGCTTTATGgt...................... : 657258 168 : >>>> >>>> Target Intron 14 >>>> LysPheLeuIleAspLysAs : 173 51914 bp :!!.!! ! !|||::!.. ++++ ++GluIleAlaGlnAspArgGl 657257 : ....aggt..........................agGAAATTGCCCAAGACCGAGG : 471939 174 : nGlyCysValValLysArgTyrGlyProMetGluGluProLeuValIleGlu{Ly} : 191 !||| ! |||! !!..|||!::|||||| :!!! ! ! !!.!! |||{||} yGlyGlyValGlySerArgTrpGlyProAspLysAlaGluAlaAlaLysGlu{Ly} 471938 : TGGAGGGGTGGGCAGCCGCTGGGGTCCTGACAAAGCAGAAGCTGCAAAGGAG{AA} : 471885 192 : >>>> Target Intron 15 >>>> {s}AspLeuPro : 194 174019 bp {|}||||||||| ++ ++{s}AspLeuPro 471884 : gt..........................ag{G}GACTTACCA : 297855 vulgar: SPP00000007_1.0 59 194 . NIVO01030317.1:subseq(555646,1182752) 1167780 297854 - 109 M 13 39 S 0 2 5 0 2 I 0 43000 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 61163 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 24337 3 0 2 S 1 1 M 19 57 5 0 2 I 0 62368 3 0 2 5 0 2 I 0 53460 3 0 2 M 5 15 S 0 1 5 0 2 I 0 31185 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 64563 3 0 2 S 1 2 M 12 36 S 0 1 5 0 2 I 0 29456 3 0 2 S 1 3 5 0 2 I 0 70626 3 0 2 S 1 2 M 7 21 5 0 2 I 0 25443 3 0 2 M 2 6 S 0 1 5 0 2 I 0 15342 3 0 2 S 1 2 M 9 27 5 0 2 I 0 29208 3 0 2 M 9 27 5 0 2 I 0 133385 3 0 2 5 0 2 I 0 51910 3 0 2 M 24 72 S 0 2 5 0 2 I 0 174015 3 0 2 S 1 1 M 3 9 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 297855 1167780 109 - . gene_id 25 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1167740 1167780 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1167740 1167780 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1167738 1167739 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1124736 1167739 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1124736 1124737 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1124727 1124735 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1124727 1124735 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1124725 1124726 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1063560 1124726 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1063560 1063561 . - . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1063530 1063559 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1063530 1063559 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1063528 1063529 . - . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1039189 1063529 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1039189 1039190 . - . intron_id 2 ; splice_site "Ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1039131 1039188 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1039131 1039188 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1039129 1039130 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1039130 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 923295 976758 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 923295 923296 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 923279 923294 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 923279 923294 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 923277 923278 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 892090 923278 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 892090 892091 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 892051 892089 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 892051 892089 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 892049 892050 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 827484 892050 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 827484 827485 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 827445 827483 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 827445 827483 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 827443 827444 . - . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 797985 827444 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 797985 797986 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 797982 797984 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 797982 797984 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 797980 797981 . - . intron_id 9 ; splice_site "ga" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 727352 797981 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 727352 727353 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 727329 727351 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 727329 727351 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 727327 727328 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 701882 727328 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 701882 701883 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 701875 701881 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 701875 701881 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 701873 701874 . - . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 686529 701874 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 686529 686530 . - . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 686500 686528 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 686500 686528 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 686498 686499 . - . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 657288 686499 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 657288 657289 . - . intron_id 11 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 657261 657287 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 657261 657287 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 657259 657260 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 657260 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 471958 523871 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 471958 471959 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 471884 471957 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 471884 471957 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 471882 471883 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297865 471883 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297865 297866 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297855 297864 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297855 297864 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 297855 1167780 109 - . alignment_id 25 ; Query SPP00000007_1.0 ; Align 1167781 60 39 ; Align 1124735 74 6 ; Align 1063559 77 27 ; Align 1039188 87 57 ; Align 923295 106 15 ; Align 892088 112 36 ; Align 827482 125 36 ; Align 727350 139 21 ; Align 701882 146 6 ; Align 686527 149 27 ; Align 657288 158 27 ; Align 471958 167 72 ; Align 297864 192 9 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 108 Query range: 12 -> 195 Target range: 1157515 -> 173705 13 : AlaLeuLeuCysGlyAlaLeuAlaAlaProGlyLeuAlaGlyThrMetCysAlaSe : 31 !.!||| !..!||||||! !|||! !||||||:!! !!|||.!! ! !:! GlyLeuThrAlaGlyAlaArgAlaGluProGlyIleProGlyAlaAlaProGlnAl 1157515 : GGGCTGACGGCCGGCGCGCGGGCGGAGCCGGGCATCCCGGGGGCTGCTCCTCAGGC : 1157461 32 : rArgAsp >>>> Target Intron 1 >>>> AspTrpArg{Cy} >>>> : 37 !||| ! 127135 bp ||||||:::{ !} aArgPro++ ++AspTrpLys{Se}++ 1157460 : GCGTCCTgt.........................aggactggaaa{ag}gt..... : 1030304 38 : Target Intron 2 >>>> {s}AlaArgSer{Me} >>>> Target Intr : 41 13714 bp {!} !|||!!!{! } 16518 b ++{r}ArgArgCys{Ar}++ 1030303 : ....................ag{T}AGGCGCTGT{AG}gt................ : 1016578 42 : on 3 >>>> {t}HisGluPheSerAla{L} >>>> Target Intron 4 > : 47 p { }|||:!! !..!|||{!} 128672 bp ++{g}HisGlnAspGluAla{A}++ 1016577 : .........ag{A}CACCAGGATGAAGCC{A}gt...................... : 1000043 48 : >>> {ys}AspIleAspGlyHisMetValAsn{Le} >>>> Target Intro : 56 {:!}.!. ! ! !||| :!!::!{||} 18206 bp ++{rg}GlnArgProValHisArgLeuSer{Le}++ 1000042 : ...ag{GG}CAGCGTCCAGTTCACCGTCTGTCC{TT}gt................. : 871343 57 : n 5 >>>> {u}AspLysTyrArgGly{Ph} >>>> Target Intron 6 > : 62 {|}||||||:!!!:! !!{.!} 75354 bp ++{u}AspLysHisGlnArg{Il}++ 871342 : ........ag{g}GACAAACACCAAAGA{AT}gt...................... : 853119 63 : >>> {e}ValCysIleValThrAsnVal{Al} >>>> Target Intron 7 : 70 {!}:!!|||:!!|||||| :!!{ } 62200 bp ++{e}LeuCysLeuValThrLeuMet{Ar}-+ 853118 : ...ag{C}CTCTGCCTAGTAACGCTAATG{AG}at..................... : 777741 71 : >>>> {a}SerGlnUnkGlyLys{T} >>>> Target Intron 8 >>>> : 76 {!}|||..!...|||:::{ } 116589 bp ++{g}SerSerAlaGlyArg{G}++ ++ 777740 : ....ag{G}AGCTCAgcggggaga{g}gt.........................ag : 598939 77 : {hr}GluValAsnTyrThr{Gl} >>>> Target Intron 9 >>>> {n}L : 83 { !}..!:!!||| ! ! !{..} 10492 bp {.} {ly}ArgIleAsnGlnLys{Se}++ ++{r}A 598938 : {GG}AGAATAAATCAGAAG{AG}gt.........................ag{c}A : 588425 84 : euValAspLeuHis{Al} >>>> Target Intron 10 >>>> {a}ArgTy : 90 !|||||||||!!.{ } 40396 bp {!}||| rgValAspLeuGln{Ar}-+ ++{g}ArgAr 588424 : GAGTTGATTTGCAA{AG}ct..........................ag{a}aggag : 548008 91 : rAlaGluCysGlyLeu >>>> Target Intron 11 >>>> ArgIleLeuA : 99 ! !! |||||| 2679 bp !:!:!!! gLeuProTrpGlyLeu++ ++TyrLeuPheI 548007 : gcTTCCTTGGGGCCTGgt..........................agTACTTGTTTA : 545302 100 : laPheProCysAsnGlnPheGlyLysGln{Gl} >>>> Target Intron 12 : 109 !!::||||||.!. |||||| {..} 68314 bp leTrpProCysGlnAlaPheGlyCysGly{Ar}+- 545301 : TCTGGCCGTGCCAAGCCTTTGgctgtggc{ag}gc..................... : 545268 110 : >>>> {u}ProGlySerAsnGlu{Gl} >>>> Target Intron 13 >>> : 115 {!}! !|||:!!...|||{..} 14052 bp ++{g}GlnGlyAlaGlyGlu{Se}++ 545267 : .....ag{A}CAGGGAGCTGGGGAA{TC}gt......................... : 476936 116 : > {u}IleLysGluPheAlaAlaGlyTyrAsnValLys >>>> Target Int : 127 {.}:::|||...||| |||... ::: ::: 3895 -+{r}LeuLysSerPheProAlaSerGlySerPheGln++ 476935 : .cg{c}cttaaatctttcccagcgtcagggtcttttcaagt............... : 462850 128 : ron 14 >>>> PheAspMetPheSerLysIleCysValAsnGlyAsp{As} > : 139 bp ||| !:!:|||:!!! !:!:... ! !|||! {!!} ++PheLeuValPheAlaMetLeuAlaSerCysGlyGly{Gl}++ 462849 : ...........agTTCCTTGTCTTTGCGATGCTGGCATCTTGTGGCGGA{GA}gt. : 458917 140 : >>> Target Intron 15 >>>> {p}AlaHisProLeuTrpLysTrpMet{L : 148 85873 bp {:}...||| ...::: |||:::{: ++{u}GlyHisArgPheTyrProTrpVal{A 458916 : .........................ag{g}ggacacaggttctatccctgggtc{c : 373021 149 : y} >>>> Target Intron 16 >>>> {s}IleGlnProLysGlyLys > : 155 :} 56505 bp {:}!!:||| !|||..!:!! r}+- ++{g}MetGlnArgLysSerGln++ 373020 : g}ga..........................ag{T}ATGCAGAGGAAGTCCCAGgt. : 316493 156 : >>> Target Intron 17 >>>> GlyIleLeuGlyAsnAlaIleLysTrpAs : 164 18516 bp ||| :!! !!:! !:!::!! !: ++GlyAlaValHisSerHisLeuGluHisSe 316492 : .........................agGGGGCGGTCCATAGCCACCTGGAACACAG : 297952 165 : nPheThr >>>> Target Intron 18 >>>> LysPheLeuIleAspLysA : 173 !.!. ! 10927 bp ||| !! !! !! !| rIleGln++ ++AlaPheGlyThrGlyThrA 297951 : CATACAGgt..........................agGCCTTTGGCACAGGCACAA : 286998 174 : snGlyCysValValLys{Ar} >>>> Target Intron 19 >>>> {g}Ty : 180 |||||! !!.!|||:!!{!:} 48398 bp {!} ! snGlyPheAlaValGlu{Ly}++ ++{s}Gl 286997 : ATGGGTTTGCTGTGGAA{AA}gt..........................ag{G}CA : 238579 181 : rGlyProMetGluGluProLeuValIleGlu{L} >>>> Target Intron 2 : 191 ||||||...:::||| !..!{ } 64826 bp nGlyProGlnGlnGluGluGlyLysAlaArg{G}++ 238578 : GGGGCCAcagcaggaggaggggaaagCCCGG{G}gt.................... : 238543 192 : 0 >>>> {ys}AspLeuProHis : 195 { !}|||||||||::: ++{ly}AspLeuProAsn 238542 : ......ag{gg}gatcttcccaac : 173706 vulgar: SPP00000007_1.0 12 195 . NIVO01030317.1:subseq(555646,1182752) 1157515 173705 - 108 M 21 63 5 0 2 I 0 127131 3 0 2 M 3 9 S 0 2 5 0 2 I 0 13710 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 16514 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 128668 3 0 2 S 1 2 M 8 24 S 0 2 5 0 2 I 0 18202 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 75350 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 62196 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 116585 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 10488 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 40392 3 0 2 S 1 1 M 7 21 5 0 2 I 0 2675 3 0 2 M 13 39 S 0 2 5 0 2 I 0 68310 3 0 2 S 1 1 M 5 15 S 0 2 5 0 2 I 0 14048 3 0 2 S 1 1 M 11 33 5 0 2 I 0 3891 3 0 2 M 12 36 S 0 2 5 0 2 I 0 85869 3 0 2 S 1 1 M 8 24 S 0 2 5 0 2 I 0 56501 3 0 2 S 1 1 M 6 18 5 0 2 I 0 18512 3 0 2 M 12 36 5 0 2 I 0 10923 3 0 2 M 12 36 S 0 2 5 0 2 I 0 48394 3 0 2 S 1 1 M 11 33 S 0 1 5 0 2 I 0 64822 3 0 2 S 1 2 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 173706 1157515 108 - . gene_id 26 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1157453 1157515 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1157453 1157515 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1157451 1157452 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1030318 1157452 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1030318 1030319 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1030307 1030317 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1030307 1030317 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1030305 1030306 . - . intron_id 2 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016593 1030306 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016593 1016594 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016592 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016592 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1000063 1016580 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1000063 1000064 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1000046 1000062 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1000046 1000062 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1000044 1000045 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 871374 1000045 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 871374 871375 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 871346 871373 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 871346 871373 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 871344 871345 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 853140 871345 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 853140 853141 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 853122 853139 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 853122 853139 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 853120 853121 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 777768 853121 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 777768 777769 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 777744 777767 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 777744 777767 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 777742 777743 . - . intron_id 7 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 715544 777743 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 715544 715545 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 715527 715543 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 715527 715543 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 715525 715526 . - . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 598938 715526 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 598938 598939 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 598919 598937 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 598919 598937 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 598917 598918 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 588427 598918 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 588427 588428 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 588409 588426 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 588409 588426 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 588407 588408 . - . intron_id 10 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 548013 588408 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 548013 548014 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 547991 548012 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 547991 548012 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 547989 547990 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 545312 547990 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 545312 545313 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 545271 545311 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 545271 545311 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 545269 545270 . - . intron_id 12 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 476957 545270 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 476957 476958 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 476939 476956 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 476939 476956 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 476937 476938 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 462887 476938 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 462887 462888 . - . intron_id 12 ; splice_site "cg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 462853 462886 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 462853 462886 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 462851 462852 . - . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 458958 462852 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 458958 458959 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 458920 458957 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 458920 458957 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 458918 458919 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 373047 458919 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 373047 373048 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 373020 373046 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 373020 373046 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 373018 373019 . - . intron_id 16 ; splice_site "ga" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 316515 373019 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 316515 316516 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 316496 316514 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 316496 316514 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 316494 316495 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297980 316495 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297980 297981 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297944 297979 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297944 297979 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297942 297943 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 287017 297943 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 287017 287018 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 286979 287016 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 286979 287016 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 286977 286978 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 238581 286978 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 238581 238582 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 238546 238580 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 238546 238580 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 238544 238545 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 173720 238545 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 173720 173721 . - . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 173706 173719 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 173706 173719 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 173706 1157515 108 - . alignment_id 26 ; Query SPP00000007_1.0 ; Align 1157516 13 63 ; Align 1030318 34 9 ; Align 1016592 38 9 ; Align 1000062 42 15 ; Align 871372 48 24 ; Align 853139 57 15 ; Align 777767 63 21 ; Align 715543 71 15 ; Align 598936 77 15 ; Align 588426 83 15 ; Align 548012 89 21 ; Align 545312 96 39 ; Align 476956 110 15 ; Align 462886 116 33 ; Align 458958 127 36 ; Align 373046 140 24 ; Align 316514 149 18 ; Align 297980 155 36 ; Align 287017 167 36 ; Align 238580 180 33 ; Align 173718 192 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 110 Query range: 0 -> 196 Target range: 1126961 -> 38581 1 : MetSerLeuGlyArgLeuCysArgLeuLeuLysProAla >>>> Target Int : 14 |||..!|||||||||||| !!:! !! !:!!|||!.! 62688 MetGluLeuGlyArgLeuLeuGlnGluCysGlnProGly++ 1126961 : ATGGAACTCGGCCGCCTCCTTCAGGAATGCCAGCCCGGGgt............... : 1126920 15 : ron 1 >>>> LeuLeuCys{G} >>>> Target Intron 2 >>>> {ly : 17 bp |||||| ! {|} 8673 bp {|| ++LeuLeuGly{G}++ ++{ly 1126919 : ..........agCTCTTGGGG{G}gt.........................ag{gg : 1055551 18 : }AlaLeuAlaAlaProGlyLeuAlaGly{Th} >>>> Target Intron 3 > : 27 }|||||| ||||||::: {! } 38931 bp }AlaLeuProAspProGlyIleGluPro{Me}++ 1055550 : }gctcttcccgacccagggatcgaaccc{at}gt...................... : 1055518 28 : >>> {r}MetCys{Al} >>>> Target Intron 4 >>>> {a}SerArg : 32 {!} |||{ } 62596 bp {!}:::::: ++{t}ArgCys{Ar}++ ++{g}AsnGln 1055517 : ...ag{G}CGCTGT{AG}gt.........................ag{g}aatcaa : 953980 33 : AspAspTrpArgCysAlaArg{Se} >>>> Target Intron 5 >>>> {r : 40 ... |||... {||} 10471 bp {| ThrGlnValSerCysThrGly{Se}++ -+{r 953979 : acccaggtctcctgcactggc{ag}gt.........................cg{C : 943483 41 : }MetHisGluPheSerAlaLysAspIleAspGlyHisMetValAsnLeuAspLysT : 59 }! !!...! !|||.!!:!!! :!!..!!.!|||||| !||||||! !!.| }LysGlnSerHisSerThrGluGlyLeuSerAlaHisMetCysAsnLeuGlyAsnT 943482 : }AAACAGTCACACTCGACTGAGGGACTTAGCGCACACATGTGCAATCTAGGAAATT : 943428 60 : yrArgGlyPheValCysIleValThrAsnValAlaSerGlnUnkGlyLysThrGlu : 77 ||||| !.!.:!! ! !!.!! !!.!:!! !..!:!!...! ! ! !!:!! yrArgLeuMetLeuIleGlnAlaLysThrLeuPheGluGluThrAspProProLys 943427 : ACCGACTCATGCTCATTCAAGCTAAAACTCTTTTTGAAGAAACAGACCCACCGAAA : 943374 78 : ValAsnTyrThrGlnLeuValAspLeuHisAlaArgTyr{A} >>>> Target : 91 ..!..! ! ! !...||| ! !|||||||||!.! !{ } 1637 ThrArgLysIleSerLeuProProLeuHisAlaHisArg{*}++ 943373 : ACCCGCAAAATATCTCTCCCACCCCTTCACGCACACCGT{T}gt............ : 943329 92 : Intron 6 >>>> {la}GluCysGlyLeuArgIleLeuAlaPheProCysAsnG : 104 54 bp { !}! ||||||! !|||||||||:!!.!! !||| !: -+{**}ValCysGlyTrpArgIleLeuSerIleLeuCysProG 943328 : .............gg{AA}GTCTGTGGGTGGCGTATTTTGTCAATTTTGTGCCCTG : 779539 105 : ln{Ph} >>>> Target Intron 7 >>>> {e}GlyLysGlnGluProGly : 111 !!{ } 34426 bp { }|||......:::||| lu{Ly}++ ++{s}GlySerSerGlnProArg 779538 : AA{AA}gt.........................ag{g}ggatcttcccaacccagg : 745092 112 : SerAsnGluGluIleLysGluPheAlaAlaGlyTyrAsnVal{Ly} >>>> Tar : 126 ...... :::||| ......|||...|||::: ...{||} GluArgThrGlnIleCysHisIleAlaGlyGlyPhePheThr{Ly}-+ 745091 : gagcgaacccagatctgccacattgcaggcggattctttact{aa}ct........ : 745043 127 : get Intron 8 >>>> {s}PheAspMetPheSer >>>> Target Intro : 132 59156 bp {|}.!. ! !!:!:!... 139825 bp ++{s}LeuLysIleTyrGln++ 745042 : .................ag{A}CTGAAGATCTACCaggt................. : 685871 133 : n 9 >>>> LysIleCys >>>> Target Intron 10 >>>> >>>> : 135 :!!:!: 22168 bp ++GluLeuGln++ ++++ 685870 : ........agGAGCTGCAGgt..........................aggt..... : 523869 136 : Target Intron 11 >>>> ValAsnGlyAspAspAlaHisProLeuTrp > : 145 16612 bp ..!!.!! !! !...||| |||||| ! ++ThrThrGluGlySerAlaThrProLeuLeu++ 523868 : .....................agACCACTGAAGGTTCAGCCACACCCTTGCTGgt. : 507227 146 : >>> Target Intron 12 >>>> LysTrpMetLysIleGlnProLysGlyLy : 154 6300 bp ||| ||| ||| !... !!: -+ProTrp***LysGluGlnPheSerGlnAr 507226 : .........................tgcccTGGTGAAAGGAGCAGTTTTCTCAAAG : 500902 155 : sGly{I} >>>> Target Intron 13 >>>> {le}LeuGly >>>> Ta : 159 !|||{:} 51824 bp {!!}|||||| gGly{V}++ ++{al}LeuGly++ 500901 : GGGA{G}gt..........................ag{TA}TTAGGGgt....... : 449061 160 : rget Intron 14 >>>> AsnAlaIleLysTrpAsnPheThrLysPheLeuIl : 170 151084 bp ...|||:!! ! ! :!!.!. ! ! !:!! ++GlyAlaValHisSerHisLeuGluHisSerIleGl 449060 : ...................agGGGGCGGTCCATAGCCACCTGGAACACAGCATACA : 297946 171 : e >>>> Target Intron 15 >>>> AspLys >>>> Target Intro : 173 141814 bp :::||| 85947 bp n++ ++GluLys++ 297945 : Ggt..........................aggaaaaggt................. : 156121 174 : n 16 >>>> AsnGly >>>> Target Intron 17 >>>> CysValVal : 177 :!!||| 10784 bp ||||||||| ++HisGly++ ++CysValVal 156120 : .........agCATGGGgt..........................agTGTGTGGTC : 59380 178 : LysArgTyrGlyProMet{G} >>>> Target Intron 18 >>>> {lu}G : 185 {|} 18219 bp {||}| IleGlyLeuGlnSerThr{G}++ ++{lu}G 59379 : atcgggctgcagtccaca{g}gt..........................ag{AG}G : 41137 186 : luProLeu >>>> Target Intron 19 >>>> ValIleGluLysAspLeu : 193 ||||| ! 2520 bp :!!!!:!!: !! !! ! luProLys++ ++LeuMetAspProGlyCys 41136 : AGCCCAAGgt..........................agCTGATGGACCCAGGTTGT : 38593 194 : ProHisTyr : 196 !|||||| PheHisTyr 38592 : TTCCATTAT : 38582 vulgar: SPP00000007_1.0 0 196 . NIVO01030317.1:subseq(555646,1182752) 1126961 38581 - 110 M 13 39 5 0 2 I 0 62684 3 0 2 M 3 9 S 0 1 5 0 2 I 0 8669 3 0 2 S 1 2 M 9 27 S 0 2 5 0 2 I 0 38927 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 62592 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 10467 3 0 2 S 1 1 M 50 150 S 0 1 5 0 2 I 0 163750 3 0 2 S 1 2 M 13 39 S 0 2 5 0 2 I 0 34422 3 0 2 S 1 1 M 20 60 S 0 2 5 0 2 I 0 59152 3 0 2 S 1 1 M 5 15 5 0 2 I 0 139821 3 0 2 M 3 9 5 0 2 I 0 22164 3 0 2 5 0 2 I 0 16608 3 0 2 M 10 30 5 0 2 I 0 6296 3 0 2 M 11 33 S 0 1 5 0 2 I 0 51820 3 0 2 S 1 2 M 2 6 5 0 2 I 0 151080 3 0 2 M 12 36 5 0 2 I 0 141810 3 0 2 M 2 6 5 0 2 I 0 85943 3 0 2 M 2 6 5 0 2 I 0 10780 3 0 2 M 9 27 S 0 1 5 0 2 I 0 18215 3 0 2 S 1 2 M 3 9 5 0 2 I 0 2516 3 0 2 M 9 27 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 38582 1126961 110 - . gene_id 27 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1126923 1126961 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1126923 1126961 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1126921 1126922 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1064235 1126922 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1064235 1064236 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1064225 1064234 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1064225 1064234 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1064223 1064224 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1055552 1064224 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1055552 1055553 . - . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1055521 1055551 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1055521 1055551 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1055519 1055520 . - . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016590 1055520 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016590 1016591 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016589 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016589 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 953985 1016580 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 953985 953986 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 953955 953984 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 953955 953984 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 953953 953954 . - . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 943484 953954 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 943484 943485 . - . intron_id 4 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 943332 943483 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 943332 943483 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 943330 943331 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 779578 943331 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 779578 779579 . - . intron_id 5 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 779535 779577 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 779535 779577 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 779533 779534 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 745109 779534 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 745109 745110 . - . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 745046 745108 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 745046 745108 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 745044 745045 . - . intron_id 8 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 685890 745045 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 685890 685891 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 685874 685889 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 685874 685889 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 685872 685873 . - . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 685873 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 546039 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 507260 523871 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 507260 507261 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 507230 507259 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 507230 507259 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 507228 507229 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 500930 507229 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 500930 500931 . - . intron_id 11 ; splice_site "tg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 500896 500929 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 500896 500929 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 500894 500895 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 449072 500895 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 449072 449073 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 449064 449071 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 449064 449071 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 449062 449063 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297980 449063 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297980 297981 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297944 297979 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297944 297979 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297942 297943 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 156130 297943 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 156130 156131 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 156124 156129 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 156124 156129 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 156122 156123 . - . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 70177 156123 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 70177 70178 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 70171 70176 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 70171 70176 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 70169 70170 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 59387 70170 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 59387 59388 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 59359 59386 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 59359 59386 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 59357 59358 . - . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41140 59358 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41140 41141 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 41129 41139 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 41129 41139 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41127 41128 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 38609 41128 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 38609 38610 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 38582 38608 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 38582 38608 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 38582 1126961 110 - . alignment_id 27 ; Query SPP00000007_1.0 ; Align 1126962 1 39 ; Align 1064235 14 9 ; Align 1055550 18 27 ; Align 1016589 28 6 ; Align 953984 31 27 ; Align 943483 41 150 ; Align 779576 92 39 ; Align 745108 106 60 ; Align 685889 127 15 ; Align 546049 132 9 ; Align 507260 135 30 ; Align 500930 145 33 ; Align 449070 157 6 ; Align 297980 159 36 ; Align 156130 171 6 ; Align 70177 173 6 ; Align 59387 175 27 ; Align 41138 185 9 ; Align 38609 188 27 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 109 Query range: 1 -> 195 Target range: 1165724 -> 32395 2 : SerLeuGlyArgLeuCysArgLeuLeuLysProAlaLeuLeuCys{Gl} >>>> : 17 ..!|||..! !||| ! ||| ! ! !|||||||||! ! !{.!} GluLeuSerAlaLeuGlyArgAlaAlaAlaProAlaLeuHisPro{Se}++ 1165724 : GAGCTCTCGGCTCTGGGACGTGCCGCTGCACCAGCTCTCCATCCC{AG}gt..... : 1165675 18 : Target Intron 1 >>>> {y}AlaLeuAlaAlaProGlyLeuAla{G} >> : 26 3153 bp {!}.!!! ! !!!.! !||||||...{|} ++{r}ThrProProGlyArgGlyLeuGly{G}++ 1165674 : ....................ag{T}ACTCCTCCCGGGAGGGGtttaggg{g}gt.. : 1162496 27 : >> Target Intron 2 >>>> {ly}ThrMetCysAla{Se} >>>> Targ : 31 24707 bp {||}|||! !|||.!!{.!} ++{ly}ThrLysCysThr{Gl}++ 1162495 : .......................ag{GG}ACGAAGTGCACT{GG}gt......... : 1137773 32 : et Intron 3 >>>> {r}ArgAspAspTrpArgCysAlaArgSerMetHisGl : 43 37177 bp {!} !! ..!|||||| ! !|||||| ! !.. ++{y}SerGlySerTrpArgLysGluArgSerArgSerAr 1137772 : ................ag{G}TCGGGGTCCTGGCGCAAGGAGAGGTCGCGGAGCCG : 1100564 44 : uPheSerAlaLys >>>> Target Intron 4 >>>> AspIleAspGlyHi : 52 ..!.||||||::: 11213 bp |||... |||:: gMetSerAlaArg++ ++AspPheProGlyAs 1100563 : CATGTCGGCTCGCgt.........................aggattttccaggcaa : 1089324 53 : sMetValAsnLeuAspLysTyrArgGlyPheVal >>>> Target Intron 5 : 64 : ......:::||| ::: ||| 59663 bp nSerThrGlyValAspCysHisPheLeuPheGln+- 1089323 : tagtactggagtggattgccatttccttttccaggg.................... : 1089286 65 : >>>> CysIleValThr{A} >>>> Target Intron 6 >>>> {sn}V : 69 |||:!:||||||{.} 11512 bp {.!} ++CysLeuValThr{G}+- ++{ly}S 1089285 : .....agTGTCTGGTAACA{G}gg.........................ag{GC}T : 1018098 70 : alAlaSerGln{U} >>>> Target Intron 7 >>>> {nk}GlyLysThr : 76 !|||||||||{ } 1493 bp { }||| ..! erAlaSerGln{V}++ ++{al}GlyAlaVal 1018097 : CTGCGTCCCAG{G}gt.........................ag{TA}GGCGCTGTA : 1016584 77 : {G} >>>> Target Intron 8 >>>> {lu}ValAsnTyr >>>> Targ : 81 {|} 25577 bp {||}! !!.. ! {G}++ ++{lu}GlyArgGln++ 1016583 : {G}gt.........................ag{AA}GGGAGGCAGgt......... : 990990 82 : et Intron 9 >>>> >>>> Target Intron 10 >>>> ThrGlnLe : 83 14234 bp 44565 bp ... ++++ ++AlaThrTh 990989 : ................aggt..........................aggcaacaac : 932187 84 : uValAspLeuHisAlaArgTyrAlaGluCysGlyLeu{Ar} >>>> Target I : 96 :::::: ||||||!:! !...||||||! !{||} 1278 rLeuGluTrpValAlaArgPheLeuSerCysGlyGln{Ar}++ 932186 : actggagtgggttgcccgtTTCCTCAGCTGCGGCCAG{AG}gt............. : 932144 97 : ntron 11 >>>> {g}IleLeuAlaPheProCysAsnGln{Ph} >>>> Tar : 105 5 bp {|}:!::!!.!!||| ! !.!...{!:} ++{g}LeuMetThrPheArgValThrSer{Tr}++ 932143 : .............ag{A}CTGATGACCTTCAGGGTGACCTCC{TG}gt........ : 919332 106 : get Intron 12 >>>> {e}GlyLysGlnGluProGlySerAsnGluGluIle : 116 4860 bp {:}||| ...:::||| :::... :::::: ++{p}GlyProSerGlnProArgAsnGlyThrGlnVal 919331 : ..................ag{g}ggaccttcccaacccaggaatggaacccaggtc : 914443 117 : LysGluPheAlaAlaGlyTyrAsnVal{Ly} >>>> Target Intron 13 > : 126 ... |||...|||::: ...{!.} 113788 bp SerCysThrAlaGlyGlyPhePheThr{Se}-+ 914442 : tcctgcactgcaggcgggttctttacc{ag}ct....................... : 914409 127 : >>> {s}PheAspMetPheSer{L} >>>> Target Intron 14 >>>> : 132 {.}... :::||||||{!} 41303 bp ++{r}IleArgValPheSer{A}++ ++ 914408 : ...ag{c}atcagggtcttttcc{a}gt..........................ag : 759305 133 : {ys}IleCysValAsnGlyAspAspAlaHisPro >>>> Target Intron 1 : 143 {:!}:::|||||| ||| |||!.! ! ! 174970 bp {rg}ValCysValCysGlyGlyAspValSerVal++ 759304 : {gg}gtgtgtgtgtgtggtggagatGTGTCTGTGgt.................... : 759269 144 : 5 >>>> LeuTrpLysTrpMet >>>> Target Intron 16 >>>> Lys : 148 !|||||| !!! ! 38238 bp :!! ++GluTrpLysArgLys++ ++Glu 759268 : ......agGAATGGAAGAGGAAGgt..........................agGAG : 546048 149 : IleGln >>>> Target Intron 17 >>>> >>>> Target Intron : 151 :!:||| 22168 bp 12126 bp LeuGln++ ++++ 546047 : CTGCAGgt..........................aggt.................. : 523869 152 : 18 >>>> ProLys >>>> Target Intron 19 >>>> GlyLysGlyI : 156 |||||| 106916 bp ||||||||| ++ProLys-+ ++GlyLysGlyG 523868 : ........agCCAAAGct..........................agGGGAAAGGGG : 404814 157 : leLeuGlyAsnAlaIleLysTrp{A} >>>> Target Intron 20 >>>> : 164 !||||||...||| {.} 12032 bp luLeuGlyGluAlaGlyAlaGly{G}++ +- 404813 : AACTcggggaggctggggctggg{g}gt..........................aa : 392759 165 : {sn}PheThrLysPheLeuIleAspLys{A} >>>> Target Intron 21 > : 173 {.!}||| |||::::::||| {:} 156732 bp {ly}PheLysLeuPheIleLeuAspTrp{A}++ 392758 : {gc}tttaaactttttattttagattgg{G}gt....................... : 392728 174 : >>> {sn}GlyCysValValLysArgTyr{G} >>>> Target Intron 22 : 181 {!!}|||...||| !!|||!.!|||{|} 5883 bp +-{sp}GlyAlaValPheLysAsnTyr{G}++ 392727 : ...at{AT}GGGGCAGTTTTCAAAAATTAT{G}gt..................... : 235972 182 : >>>> {ly}ProMetGluGluProLeuValIle >>>> Target Intron : 190 {||}|||:!:|||! ! !!! ! !!!: 73936 bp ++{ly}ProValGluAlaSerSerHisMet++ 235971 : .....ag{gg}cctgTAGAGGCATCATCTCACATGgt................... : 230063 191 : 23 >>>> GluLys >>>> Target Intron 24 >>>> >>>> Targ : 192 |||||| 114175 bp ++GluLys++ ++++ 230062 : .......aggaaaaggt..........................aggt......... : 41946 193 : et Intron 25 >>>> AspLeuProHis : 195 9541 bp ||||||||| ++ArgLeuProHis 41945 : .................agAGGCTCCCTCAC : 32396 vulgar: SPP00000007_1.0 1 195 . NIVO01030317.1:subseq(555646,1182752) 1165724 32395 - 109 M 15 45 S 0 2 5 0 2 I 0 3149 3 0 2 S 1 1 M 8 24 S 0 1 5 0 2 I 0 24703 3 0 2 S 1 2 M 4 12 S 0 2 5 0 2 I 0 37173 3 0 2 S 1 1 M 16 48 5 0 2 I 0 11209 3 0 2 M 16 48 5 0 2 I 0 59659 3 0 2 M 4 12 S 0 1 5 0 2 I 0 11508 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 1489 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 25573 3 0 2 S 1 2 M 3 9 5 0 2 I 0 14230 3 0 2 5 0 2 I 0 44561 3 0 2 M 15 45 S 0 2 5 0 2 I 0 12781 3 0 2 S 1 1 M 8 24 S 0 2 5 0 2 I 0 4856 3 0 2 S 1 1 M 20 60 S 0 2 5 0 2 I 0 113784 3 0 2 S 1 1 M 5 15 S 0 1 5 0 2 I 0 41299 3 0 2 S 1 2 M 10 30 5 0 2 I 0 174966 3 0 2 M 5 15 5 0 2 I 0 38234 3 0 2 M 3 9 5 0 2 I 0 22164 3 0 2 5 0 2 I 0 12122 3 0 2 M 2 6 5 0 2 I 0 106912 3 0 2 M 11 33 S 0 1 5 0 2 I 0 12028 3 0 2 S 1 2 M 8 24 S 0 1 5 0 2 I 0 156728 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 5879 3 0 2 S 1 2 M 8 24 5 0 2 I 0 73932 3 0 2 M 2 6 5 0 2 I 0 114171 3 0 2 5 0 2 I 0 9537 3 0 2 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 32396 1165724 109 - . gene_id 28 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1165678 1165724 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1165678 1165724 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1165676 1165677 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1162525 1165677 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1162525 1162526 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1162499 1162524 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1162499 1162524 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1162497 1162498 . - . intron_id 2 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1137792 1162498 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1137792 1137793 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1137776 1137791 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1137776 1137791 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1137774 1137775 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1100599 1137775 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1100599 1100600 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1100550 1100598 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1100550 1100598 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1100548 1100549 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1089337 1100549 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1089337 1089338 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1089289 1089336 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1089289 1089336 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1089287 1089288 . - . intron_id 5 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1029626 1089288 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1029626 1029627 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1029613 1029625 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1029613 1029625 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1029611 1029612 . - . intron_id 6 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1018101 1029612 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1018101 1018102 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1018086 1018100 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1018086 1018100 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1018084 1018085 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016593 1018085 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016593 1016594 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016592 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016592 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 991004 1016580 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 991004 991005 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 990993 991003 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 990993 991003 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 990991 990992 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 990992 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 932194 976758 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 932194 932195 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 932147 932193 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 932147 932193 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 932145 932146 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 919362 932146 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 919362 919363 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 919335 919361 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 919335 919361 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 919333 919334 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 914475 919334 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 914475 914476 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 914412 914474 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 914412 914474 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 914410 914411 . - . intron_id 13 ; splice_site "ct" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 800624 914411 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 800624 800625 . - . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 800607 800623 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 800607 800623 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 800605 800606 . - . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 759304 800606 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 759304 759305 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 759272 759303 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 759272 759303 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 759270 759271 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 584302 759271 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 584302 584303 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 584287 584301 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 584287 584301 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 584285 584286 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 584286 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 546039 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 511746 523871 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 511746 511747 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 511740 511745 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 511740 511745 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 511738 511739 . - . intron_id 19 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 404824 511739 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 404824 404825 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 404790 404823 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 404790 404823 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 404788 404789 . - . intron_id 20 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 392758 404789 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 392758 392759 . - . intron_id 19 ; splice_site "aa" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 392731 392757 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 392731 392757 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 392729 392730 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 235999 392730 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 235999 236000 . - . intron_id 20 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 235975 235998 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 235975 235998 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 235973 235974 . - . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 230092 235974 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 230092 230093 . - . intron_id 21 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 230066 230091 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 230066 230091 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 230064 230065 . - . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 156130 230065 . - . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 156130 156131 . - . intron_id 22 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 156124 156129 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 156124 156129 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 156122 156123 . - . intron_id 24 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 156123 . - . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 23 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 25 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 32408 41948 . - . intron_id 25 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 32408 32409 . - . intron_id 24 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 32396 32407 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 32396 32407 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 32396 1165724 109 - . alignment_id 28 ; Query SPP00000007_1.0 ; Align 1165725 2 45 ; Align 1162524 18 24 ; Align 1137790 27 12 ; Align 1100598 32 48 ; Align 1089337 48 48 ; Align 1029626 64 12 ; Align 1018099 69 12 ; Align 1016591 74 9 ; Align 991002 78 9 ; Align 932194 81 45 ; Align 919361 97 24 ; Align 914474 106 60 ; Align 800623 127 15 ; Align 759302 133 30 ; Align 584302 143 15 ; Align 546049 148 9 ; Align 511746 151 6 ; Align 404824 153 33 ; Align 392756 165 24 ; Align 235997 174 21 ; Align 230090 182 24 ; Align 156130 190 6 ; Align 32408 192 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 107 Query range: 1 -> 164 Target range: 1117864 -> 40258 2 : SerLeuGlyArgLeu >>>> Target Intron 1 >>>> CysArgLeuLeu : 10 ||||||||||||||| 47873 bp |||||| ::: SerLeuGlyArgLeu++ ++CysArgLysVal 1117864 : AGCCTGGGGAGGCTGgt.........................agtgcaggaaagtg : 1069967 11 : LysProAla{Le} >>>> Target Intron 2 >>>> {u}LeuCys{Gl} : 17 ||| |||{ } 53364 bp {!}! !|||{ !} LysSerAla{Ar}+- ++{g}ArgCys{Ar}+ 1069966 : aaaagtgca{ag}gc.........................ag{G}CGCTGT{AG}g : 1016580 18 : >>>> Target Intron 3 >>>> {y}AlaLeuAlaAlaPro >>>> Tar : 23 843 bp {!}:!!||| !!:!!||| + ++{g}SerLeuProSerPro++ 1016579 : t.........................ag{G}TCACTGCCCTCCCCGgt........ : 1015719 24 : get Intron 4 >>>> >>>> Target Intron 5 >>>> GlyLeuAl : 25 38963 bp 15107 bp !||||| ++++ ++HisLeuAl 1015718 : .................aggt.........................agCACCTCGC : 961645 26 : aGlyThr{M} >>>> Target Intron 6 >>>> {et}CysAlaSerArgA : 33 |!.!|||{ } 162595 bp { }|||::: :::: aAlaThr{G}++ -+{ly}CysSerArgGlnG 961644 : GGCTACA{G}gt.........................gg{ga}tgctccaggcaag : 799026 34 : spAspTrp{A} >>>> Target Intron 7 >>>> {rg}CysAlaArgSer : 40 ::||||||{|} 38880 bp {||}||| ! !! !! luAspTrp{A}++ ++{rg}CysLeuTrpPro 799025 : aagactgg{a}gt.........................ag{GA}TGCCTCTGGCCA : 760125 41 : MetHisGluPheSerAlaLysAsp{Il} >>>> Target Intron 8 >>>> : 49 !||| !!!! !:!!.!.{! } 117691 bp AlaHisLeuAlaThrAspGluGln{Th}++ + 760124 : GCGCATCTCGCAACTGATGAACAA{AC}gt.........................a : 642407 50 : {e}AspGlyHisMetValAsn >>>> Target Intron 9 >>>> >>> : 56 {!}|||! !|||:!!:!!.!. 118515 bp +{r}AspAspHisValLeuGlu++ ++++ 642406 : g{A}GATGACCACGTGCTGGAGgt.........................aggt... : 523869 57 : > Target Intron 10 >>>> Leu >>>> Target Intron 11 >>>> : 57 28329 bp ||| 13892 bp ++Leu++ 523868 : .......................agCTGgt.......................... : 495537 58 : AspLysTyrArgGlyPheValCysIleValThrAsn >>>> Target Intr : 69 ! !!. !|||..!||| !|||:!:||| !..! 4488 b ++AlaAsnProArgSerPheLysCysValValLeuArg++ 495536 : agGCGAATCCCAGGTCCTTCAAATGTGTGGTCCTGCGTgt................ : 481609 70 : on 12 >>>> ValAlaSerGln >>>> Target Intron 13 >>>> Un : 73 p ..!!.!..!||| 49969 bp .. ++ThrGlyGluGln++ ++Th 481608 : ..........agACTGGTGAACAAgt..........................agAC : 427142 74 : kGlyLysThrGluValAsnTyrThrGlnLeuVal >>>> Target Intron 1 : 85 .|||||| ... ||| |||::: 98040 bp rGlyLysGlnArgGlnAsnLysThrGluSerGlu++ 427141 : agggaaacagagacagaataaaacagaaagtgaagt.................... : 427104 86 : 4 >>>> AspLeuHisAlaArgTyrAlaGluCys{G} >>>> Target Intr : 94 ||||||!!.!.!!:!:!!!.!... !{|} 55519 b ++AspLeuGlnValLysHisGlySerAsp{G}++ 427103 : ......agGACTTGCAAGTAAAACACGGTAGTGAT{G}gt................ : 329036 95 : on 15 >>>> {ly}LeuArgIleLeuAlaPheProCysAsnGln{P} >>>> : 105 p {||}! ||| ...|||||||||::!{!} ++{ly}***ArgProCysProLeuProCysAsnArg{S}+- 329035 : ..........ag{GG}TGaaggccctgccccctcccttgtAACAGA{T}gg..... : 273484 106 : Target Intron 16 >>>> {he}GlyLysGlnGluPro{G} >>>> Targ : 111 82827 bp { }|||||| ...|||{|} ++{er}GlyLysLeuArgPro{G}++ 273483 : .....................ag{ca}gggaaactgaggcct{g}gt......... : 190639 112 : et Intron 17 >>>> {ly}SerAsnGluGluIleLysGluPheAlaAlaGly : 122 108241 bp {||}.!!:!!! !||| ! !:: ! !||| ++{ly}GlyHisValGluGlnAspCysTrpLeuArgGly 190638 : .................ag{GT}GGACACGTGGAGCAGGACTGCTGGCTCCGAGGT : 82368 123 : TyrAsnValLys{Ph} >>>> Target Intron 18 >>>> {e}AspMetP : 130 ||| !||| { } 15475 bp { }! ! !! TyrCysValAla{Gl}++ +-{y}Gly***T 82367 : TACTGTGTAGCC{GG}gt..........................ac{A}GGTTAGT : 66869 131 : heSerLysIleCysValAsnGlyAspAspAlaHisProLeuTrpLysTrpMetLys : 148 ::..!||| ! !!:!! !|||..! !!!!.||| !||| !||| ... rpAspLysCysSerLeuProGlySerProProGlnProThrTrpLeuTrpProSer 66868 : GGGACAAATGCAGCCTTCCTGGCAGCCCGCCTCAGCCCACTTGGCTGTGGCCCTCC : 66815 149 : IleGlnProLysGly{Ly} >>>> Target Intron 19 >>>> {s}GlyI : 156 !!!.|||!..|||{:!} 26506 bp {!}|||: AspHisProSerGly{Gl}++ -+{n}GlyL 66814 : GACCACCCTAGTGGG{CA}gt..........................tg{G}GGGC : 40285 157 : leLeuGlyAsnAlaIleLysTrpAsn : 164 !!||||||::!!.!:!! !|||!:! euLeuGlySerValValProTrpSer 40284 : TCCTGGGGTCTGTCGTCCCATGGAGC : 40259 vulgar: SPP00000007_1.0 1 164 . NIVO01030317.1:subseq(555646,1182752) 1117864 40258 - 107 M 5 15 5 0 2 I 0 47869 3 0 2 M 7 21 S 0 2 5 0 2 I 0 53360 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 839 3 0 2 S 1 1 M 5 15 5 0 2 I 0 38959 3 0 2 5 0 2 I 0 15103 3 0 2 M 5 15 S 0 1 5 0 2 I 0 162591 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 38876 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 117687 3 0 2 S 1 1 M 6 18 5 0 2 I 0 118511 3 0 2 5 0 2 I 0 28325 3 0 2 M 1 3 5 0 2 I 0 13888 3 0 2 M 12 36 5 0 2 I 0 4484 3 0 2 M 4 12 5 0 2 I 0 49965 3 0 2 M 12 36 5 0 2 I 0 98036 3 0 2 M 9 27 S 0 1 5 0 2 I 0 55515 3 0 2 S 1 2 M 10 30 S 0 1 5 0 2 I 0 82823 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 108237 3 0 2 S 1 2 M 15 45 S 0 2 5 0 2 I 0 15471 3 0 2 S 1 1 M 26 78 S 0 2 5 0 2 I 0 26502 3 0 2 S 1 1 M 10 30 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 40259 1117864 107 - . gene_id 29 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1117850 1117864 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1117850 1117864 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1117848 1117849 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1069977 1117849 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1069977 1069978 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1069954 1069976 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1069954 1069976 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1069952 1069953 . - . intron_id 2 ; splice_site "gc" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016590 1069953 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016590 1016591 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016589 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016589 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1015738 1016580 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1015738 1015739 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1015722 1015737 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1015722 1015737 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1015720 1015721 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1015721 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 961652 976758 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 961652 961653 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 961636 961651 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 961636 961651 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 961634 961635 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 799041 961635 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 799041 799042 . - . intron_id 5 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 799017 799040 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 799017 799040 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 799015 799016 . - . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 760137 799016 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 760137 760138 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 760097 760136 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 760097 760136 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 760095 760096 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 642406 760096 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 642406 642407 . - . intron_id 7 ; splice_site "aG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 642387 642405 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 642387 642405 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 642385 642386 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 642386 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 495543 523871 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 495543 495544 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 495540 495542 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 495540 495542 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 495538 495539 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 481648 495539 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 481648 481649 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 481612 481647 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 481612 481647 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 481610 481611 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 481611 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 427143 477111 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 427143 427144 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 427107 427142 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 427107 427142 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 427105 427106 . - . intron_id 14 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 329067 427106 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 329067 329068 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 329039 329066 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 329039 329066 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 329037 329038 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 273520 329038 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 273520 273521 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 273487 273519 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 273487 273519 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 273485 273486 . - . intron_id 16 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 190660 273486 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 190660 190661 . - . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 190642 190659 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 190642 190659 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 190640 190641 . - . intron_id 17 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 82401 190641 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 82401 82402 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 82352 82400 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 82352 82400 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 82350 82351 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 66877 82351 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 66877 66878 . - . intron_id 17 ; splice_site "AC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 66796 66876 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 66796 66876 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 66794 66795 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 40290 66795 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 40290 40291 . - . intron_id 18 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 40259 40289 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 40259 40289 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 40259 1117864 107 - . alignment_id 29 ; Query SPP00000007_1.0 ; Align 1117865 2 15 ; Align 1069977 7 21 ; Align 1016589 15 6 ; Align 1015737 18 15 ; Align 961652 23 15 ; Align 799039 29 21 ; Align 760135 37 36 ; Align 642405 50 18 ; Align 495543 56 3 ; Align 481648 57 36 ; Align 477124 69 12 ; Align 427143 73 36 ; Align 329067 85 27 ; Align 273518 95 30 ; Align 190658 106 15 ; Align 82399 112 45 ; Align 66876 128 78 ; Align 40289 155 30 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 107 Query range: 0 -> 142 Target range: 1128210 -> 31072 1 : MetSerLeuGlyArg >>>> Target Intron 1 >>>> Leu{C} >>>> : 7 ..!.!!||||||||| 151437 bp :!!{|} GlnGlyLeuGlyArg++ ++Val{C}++ 1128210 : CAGGGACTCGGGAGGgt.........................agGTA{T}gt.... : 976752 8 : Target Intron 2 >>>> {ys}ArgLeuLeuLys{P} >>>> Target : 12 175613 bp {||}||||||......{ } 908 ++{ys}ArgLeuPheAsn{A}++ 976751 : .....................ag{gc}agactgtttaac{G}gt............ : 801124 13 : Intron 3 >>>> {ro}AlaLeuLeuCysGly{Al} >>>> Target Intr : 18 04 bp {!!}:!!! !|||||||||{ } 46725 b ++{la}SerArgLeuCysGly{Ar}+- 801123 : .............ag{CG}TCACGACTTTGTGGA{CG}ga................ : 710301 19 : on 4 >>>> {a}Leu >>>> Target Intron 5 >>>> >>>> Tar : 20 p {!}||| 139703 bp ++{g}Leu++ ++++ 710300 : .........ag{C}CTGgt.........................aggt........ : 523869 21 : get Intron 6 >>>> AlaAlaProGlyLeuAlaGlyThrMetCysAlaSerA : 32 7580 bp ||| !!||| !! ! !|||! !:!!||| ! !!| ++AlaProProProTrpLeuGlyIleLeuCysLeuProA 523868 : .................agGCACCCCCGCCATGGTTGGGTATCCTGTGTCTCCCCA : 516255 33 : rgAspAsp >>>> Target Intron 7 >>>> TrpArgCysAlaArg >> : 40 ||||| ! 2932 bp ||| ||| rgAspLys+- ++TrpSerCysMetAsp++ 516254 : GAGACAAGgg.........................agtggtcatgtatggatgt.. : 513297 41 : >> Target Intron 8 >>>> SerMetHisGluPheSer{Al} >>>> Ta : 46 43814 bp |||..! !..!||||||{::} ++SerGlnAlaArgPheSer{Se}++ 513296 : .......................agTCACAGGCTAGATTCTCG{AG}gt....... : 469463 47 : rget Intron 9 >>>> {a}LysAspIleAsp{Gl} >>>> Target Int : 51 168153 bp {!}:!! !!:!:!!:{.!} 39780 ++{r}GlnHisLeuGlu{Se}++ 469462 : ..................ag{C}CAACACCTGGAA{AG}gt............... : 301295 52 : ron 10 >>>> {y}HisMetValAsnLeuAspLysTyrArgGlyPheValCysI : 65 bp {!}...:!: !!:!:!!||| ! !!..!.!.||||||: ++{r}ArgValLysSerValAspGlySerTrpSerLeuValCysL 301294 : ...........ag{C}AGAGTCAAGAGTGTGGATGGCAGCTGGTCTCTGGTCTGCC : 261477 66 : leValThrAsnValAlaSerGlnUnkGlyLysThrGluValAsnTyrThrGlnLeu : 83 !!..!!.!!.! ! !|||||| !!:!||||||!.!.!. ! |||||| ! euThrAsnThrTrpPheSerGlnLysHisArgThrGluAlaGlnGlnThrGlnGlu 261476 : TCACAAACACCTGGTTTTCTCAAAAACACAGAACAGAGGCACAGCAAACTCAGGAG : 261423 84 : Val >>>> Target Intron 11 >>>> AspLeuHisAlaArgTyrAlaGl : 92 ! 56398 bp !!|||...!.!|||||| !:! Pro++ ++HisLeuArgGlyArgTyrIleGl 261422 : CCGgt..........................agCACCTGAGAGGCAGGTACATACA : 204998 93 : u >>>> Target Intron 12 >>>> CysGlyLeu{Ar} >>>> Targe : 96 ! 12431 bp |||..!:!!{ !} 3 n++ ++CysAsnMet{Gl}++ 204997 : Ggt..........................agTGCAACATG{GG}gt.......... : 192551 97 : t Intron 13 >>>> {g}IleLeuAlaPheProCysAsnGlnPheGlyLysGl : 108 2742 bp {!} !||| ! ||||||:!!||| !||| !! ++{y}HisLeuArgThrProCysHisGlnHisGlyAlaPr 192550 : ................ag{A}CATCTGCGCACGCCGTGCCACCAACATGGGGCACC : 159777 109 : nGluProGlySer{A} >>>> Target Intron 14 >>>> {sn}GluGlu : 115 ! |||..!|||{:} 95421 bp {::} .!. oPheProSerSer{S}++ ++{er}ProHis 159776 : ATTTCCCTCTTCC{T}gt..........................ag{CA}CCCCAC : 64335 116 : IleLysGluPheAla{Al} >>>> Target Intron 15 >>>> {a}GlyT : 123 .!!..!..! !.!!{::} 11878 bp {!}||| PheSerSerAlaThr{Se}++ ++{r}GlyV 64334 : TTCTCATCAGCTACC{AG}gt..........................ag{T}GGGG : 52433 124 : yrAsnVal{Ly} >>>> Target Intron 16 >>>> {s}PheAspMetPh : 130 |||{!:} 666 bp {!}.!.! ! !! alTrpVal{Ar}++ ++{g}MetAlaProLe 52432 : TGTGGGTG{AG}gt..........................ag{G}ATGGCCCCTTT : 51746 131 : eSerLys{Il} >>>> Target Intron 17 >>>> {e}CysValAsn{G} : 137 .|||!..{! } 13440 bp { }||| !..!{|} uSerSer{Ar}++ ++{g}CysCysGly{G} 51745 : GAGCAGC{AG}gt..........................ag{G}TGCTGTGGT{G} : 38285 138 : >>>> Target Intron 18 >>>> {ly}AspAspAlaHisPro : 142 7195 bp {||}|||||||||:!!||| ++ ++{ly}AspAspAlaTyrPro 38284 : gt..........................ag{gc}gacGACGCTTATCCA : 31073 vulgar: SPP00000007_1.0 0 142 . NIVO01030317.1:subseq(555646,1182752) 1128210 31072 - 107 M 5 15 5 0 2 I 0 151433 3 0 2 M 1 3 S 0 1 5 0 2 I 0 175609 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 90800 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 46721 3 0 2 S 1 1 M 1 3 5 0 2 I 0 139699 3 0 2 5 0 2 I 0 7576 3 0 2 M 15 45 5 0 2 I 0 2928 3 0 2 M 5 15 5 0 2 I 0 43810 3 0 2 M 6 18 S 0 2 5 0 2 I 0 168149 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 39776 3 0 2 S 1 1 M 33 99 5 0 2 I 0 56394 3 0 2 M 8 24 5 0 2 I 0 12427 3 0 2 M 3 9 S 0 2 5 0 2 I 0 32738 3 0 2 S 1 1 M 16 48 S 0 1 5 0 2 I 0 95417 3 0 2 S 1 2 M 7 21 S 0 2 5 0 2 I 0 11874 3 0 2 S 1 1 M 4 12 S 0 2 5 0 2 I 0 662 3 0 2 S 1 1 M 6 18 S 0 2 5 0 2 I 0 13436 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 7191 3 0 2 S 1 2 M 5 15 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 31073 1128210 107 - . gene_id 30 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1128196 1128210 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1128196 1128210 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1128194 1128195 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1128195 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 976755 976758 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 976755 976758 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976753 976754 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 801142 976754 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 801142 801143 . - . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 801127 801141 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 801127 801141 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 801125 801126 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 710323 801126 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 710323 710324 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 710304 710322 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 710304 710322 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 710302 710303 . - . intron_id 4 ; splice_site "GA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 663579 710303 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 663579 663580 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 663575 663578 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 663575 663578 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 663573 663574 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 663574 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 516292 523871 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 516292 516293 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 516247 516291 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 516247 516291 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 516245 516246 . - . intron_id 7 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 513315 516246 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 513315 513316 . - . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 513300 513314 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 513300 513314 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 513298 513299 . - . intron_id 8 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 469486 513299 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 469486 469487 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 469466 469485 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 469466 469485 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 469464 469465 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 301313 469465 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 301313 301314 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 301298 301312 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 301298 301312 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 301296 301297 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 261518 301297 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 261518 261519 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 261418 261517 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 261418 261517 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 261416 261417 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 205020 261417 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 205020 205021 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 204996 205019 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 204996 205019 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 204994 204995 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 192565 204995 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 192565 192566 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 192554 192564 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 192554 192564 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 192552 192553 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 159812 192553 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 159812 159813 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 159762 159811 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 159762 159811 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 159760 159761 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64341 159761 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64341 64342 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64316 64340 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64316 64340 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64314 64315 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 52438 64315 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 52438 52439 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 52423 52437 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 52423 52437 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 52421 52422 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 51757 52422 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 51757 51758 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 51736 51756 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 51736 51756 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 51734 51735 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 38296 51735 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 38296 38297 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 38285 38295 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 38285 38295 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 38283 38284 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 31090 38284 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 31090 31091 . - . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 31073 31089 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 31073 31089 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 31073 1128210 107 - . alignment_id 30 ; Query SPP00000007_1.0 ; Align 1128211 1 15 ; Align 976759 6 3 ; Align 801140 8 12 ; Align 710321 13 15 ; Align 663578 19 3 ; Align 516292 20 45 ; Align 513315 35 15 ; Align 469486 40 18 ; Align 301312 47 12 ; Align 261517 52 99 ; Align 205020 85 24 ; Align 192565 93 9 ; Align 159811 97 48 ; Align 64339 114 21 ; Align 52437 122 12 ; Align 51756 127 18 ; Align 38295 134 9 ; Align 31088 138 15 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 110 Query range: 4 -> 186 Target range: 1160072 -> 13414 5 : ArgLeuCysArgLeuLeu{Ly} >>>> Target Intron 1 >>>> {s}Pr : 12 ||| !||| !!||||||{!:} 113024 bp {!}|| ArgArgCys***LeuLeu{Ar}++ ++{g}Pr 1160072 : AGAAGATGCTGACTCCTA{AG}gt.........................ag{G}CC : 1047027 13 : oAlaLeuLeuCysGlyAlaLeuAlaAlaProGly >>>> Target Intron 2 : 24 |!.!|||! ! !! !!!.! !!.!!.!|||! ! 70233 bp oValLeuTyrArgArgValAlaGlyGlyProGlu++ 1047026 : CGTTCTTTACCGCCGAGTGGCCGGGGGTCCTGAGgt.................... : 1046989 25 : >>>> >>>> Target Intron 3 >>>> LeuAlaGlyThrMetCysAl : 30 186691 bp :!!:!!||| !..!..! ++++ ++MetSerGlyLeuGlnAlaLe 1046988 : .....aggt.........................agATGTCCGGTCTCCAGGCCTT : 790049 31 : aSerArgAspAspTrp{Ar} >>>> Target Intron 4 >>>> {g}CysA : 38 !.!! !!|||! |||{||} 20323 bp {|} . uGlyGlyAspGlyTrp{Ar}++ ++{g}GluG 790048 : GGGGGGCGATGGATGG{AG}gt.........................ag{g}gagg : 769702 39 : laArgSerMetHis >>>> Target Intron 5 >>>> GluPheSerAlaL : 47 .. ...|||||| 185386 bp |||!::!.! !| lyTrpGlyMetHis++ ++GluTrpLysArgL 769701 : ggtgggggatgcATgt.........................agGAATGGAAGAGGA : 584289 48 : ys >>>> Target Intron 6 >>>> AspIleAsp >>>> Target In : 51 || 38238 bp !!::!:.!. 22168 ys++ ++GluLeuGln++ 584288 : AGgt.........................agGAGCTGCAGgt.............. : 546037 52 : tron 7 >>>> >>>> Target Intron 8 >>>> Gly{Hi} >>>> : 52 bp 22971 bp |||{..} ++++ ++Gly{Ar}++ 546036 : ...........aggt.........................agGGG{AG}gt..... : 500893 53 : Target Intron 9 >>>> {s}MetValAsn{Le} >>>> Target Intr : 56 23772 bp {.}:!!||||||{ } 125075 ++{g}LeuValAsn{Ly}++ 500892 : ....................ag{A}CTGGTGAAC{AA}gt................ : 477109 57 : on 10 >>>> {u}AspLys{Ty} >>>> Target Intron 11 >>>> { : 59 bp {!}||| !{ !} 5797 bp { ++{s}AspAla{Ly}++ ++{ 477108 : ..........ag{A}GACGCA{AA}gt..........................ag{ : 346230 60 : r}ArgGlyPheValCysIleValThrAsn{Va} >>>> Target Intron 12 : 69 }!:! !.!!||||||:!: !|||! !{ } 48221 bp s}LysHisLeuValCysLeuSerThrIle{Tr}++ 346229 : G}AAACACCTTGTGTGTCTGTCTACAATC{TG}gt..................... : 346198 70 : >>>> {l}AlaSerGlnUnkGlyLysThrGluValAsnTyr{Th} >>>> Ta : 81 {!}!.! !!... !.!! !.!...! |||{! } ++{p}GlyArgSerIleAlaThrTrpAsnThrAlaTyr{Ar}++ 346197 : .....ag{G}GGGCGGTCCATAGCCACCTGGAACACAGCATAC{AG}gt....... : 297941 82 : rget Intron 13 >>>> {r}GlnLeuValAspLeuHisAlaArgTyr >>> : 91 29447 bp {!}.!.||||||..!||| !!.!!:! ! ++{g}AspLeuValSerLeuSerGlyLysGln++ 297940 : ...................ag{G}GACTTGGTCTCCTTATCTGGAAAACAGgt... : 268466 92 : > Target Intron 14 >>>> AlaGluCysGlyLeu{Ar} >>>> Targe : 96 74528 bp |||..!||||||! !{||} 1 ++AlaSerCysGlyPro{Ar}++ 268465 : .......................agGCCTCATGTGGCCCA{AG}gt.......... : 193921 97 : t Intron 15 >>>> {g}IleLeuAlaPheProCysAsnGlnPheGlyLysGl : 108 3937 bp {|}:!:! !!.! !||| !! !||| !.!! ! || ++{g}ValArgGlyHisProGlyLeuGlnThrSerHisGl 193920 : ................ag{A}GTGCGTGGCCATCCTGGCCTCCAGACCAGCCATCA : 179952 109 : nGlu >>>> Target Intron 16 >>>> Pro >>>> Target Intro : 111 |||| 12926 bp ||| 48987 bp nGlu++ ++Pro++ 179951 : GGAGgt..........................agCCCgt................. : 167015 112 : n 17 >>>> GlySerAsnGluGlu{I} >>>> Target Intron 18 >>> : 116 ||||||...:!!..!{ } 22606 bp ++GlySerGlyGlnArg{A}++ 167014 : .........agGGGTCGGGGCAGAGG{G}gt......................... : 118012 117 : > {le}LysGluPheAlaAlaGlyTyrAsnValLysPheAspMetPheSerLysI : 133 { !}... ||| !! !!||| ! ! ! !!:!! !! !:!:! |||!..| ++{la}SerPhePheProProGlyGluIleHisArgCysGlyLeu***SerSerI 118011 : .ag{CC}TCCTTCTTTCCACCAGGTGAAATTCACAGATGTGGCCTCTAGTCCAGCA : 95358 134 : leCysValAsnGly{A} >>>> Target Intron 19 >>>> {sp}AspAl : 140 |||||! !!....!{|} 14483 bp {||} !:: leCysGlyArgSer{A}++ ++{sp}ThrSe 95357 : TATGTGGCAGATCA{G}gt..........................ag{AC}ACTAG : 80854 141 : aHisProLeuTrp{L} >>>> Target Intron 20 >>>> {ys}TrpMet : 147 !||| !! ! !!{!} 19476 bp {:!}||| rHisArgSerGly{A}++ ++{rg}TrpSer 80853 : TCACAGATCTGGG{A}gt..........................ag{GG}TGGTCT : 61357 148 : LysIleGlnProLys >>>> Target Intron 21 >>>> GlyLysGlyIl : 156 ! ! ! !|||:!! 8845 bp |||...|||:: MetProAlaProGln++ ++GlySerGlyVa 61356 : ATGCCAGCGCCACAGgt..........................agGGcagcggggt : 52485 157 : eLeuGlyAsnAlaIleLys >>>> Target Intron 22 >>>> >>>> : 163 : |||...|||!!: ! 10516 bp lGlyGlyGlyAlaMetVal++ ++++ 52484 : gggagggggggcaATGGTGgt..........................aggt..... : 41946 164 : Target Intron 23 >>>> TrpAsnPheThrLysPheLeuIleAspLysAsn : 173 23371 bp ||| .!.||| ! ! !! !:!!||| ++TrpValLeuThrAlaArgAlaProGlyGluAsn 41945 : .....................agTGGGTACTGACAGCAAGAGCACCTGGTGAGAAT : 18547 174 : GlyCysValValLys{A} >>>> Target Intron 24 >>>> {rg}TyrG : 181 ..!||| !!.! ! {|} 5091 bp {||} . SerCysTrpAlaHis{A}++ ++{rg}LeuS 18546 : TCATGCTGGGCACAC{A}gt..........................ag{GG}CTAT : 13432 182 : lyProMetGluGluPro : 186 .!|||:!!! !|||||| erProLeuAlaGluPro 13431 : CTCCTCTGGCGGAACCG : 13415 vulgar: SPP00000007_1.0 4 186 . NIVO01030317.1:subseq(555646,1182752) 1160072 13414 - 110 M 6 18 S 0 2 5 0 2 I 0 113020 3 0 2 S 1 1 M 12 36 5 0 2 I 0 70229 3 0 2 5 0 2 I 0 186687 3 0 2 M 12 36 S 0 2 5 0 2 I 0 20319 3 0 2 S 1 1 M 6 18 5 0 2 I 0 185382 3 0 2 M 5 15 5 0 2 I 0 38234 3 0 2 M 3 9 5 0 2 I 0 22164 3 0 2 5 0 2 I 0 22967 3 0 2 M 1 3 S 0 2 5 0 2 I 0 23768 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 125071 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 5793 3 0 2 S 1 1 M 9 27 S 0 2 5 0 2 I 0 48217 3 0 2 S 1 1 M 11 33 S 0 2 5 0 2 I 0 29443 3 0 2 S 1 1 M 9 27 5 0 2 I 0 74524 3 0 2 M 5 15 S 0 2 5 0 2 I 0 13933 3 0 2 S 1 1 M 13 39 5 0 2 I 0 12922 3 0 2 M 1 3 5 0 2 I 0 48983 3 0 2 M 5 15 S 0 1 5 0 2 I 0 22602 3 0 2 S 1 2 M 21 63 S 0 1 5 0 2 I 0 14479 3 0 2 S 1 2 M 6 18 S 0 1 5 0 2 I 0 19472 3 0 2 S 1 2 M 7 21 5 0 2 I 0 8841 3 0 2 M 10 30 5 0 2 I 0 10512 3 0 2 5 0 2 I 0 23367 3 0 2 M 16 48 S 0 1 5 0 2 I 0 5087 3 0 2 S 1 2 M 7 21 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 13415 1160072 110 - . gene_id 31 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1160053 1160072 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1160053 1160072 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1160051 1160052 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1047029 1160052 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1047029 1047030 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1046992 1047028 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1046992 1047028 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1046990 1046991 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1046991 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 790068 976758 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 790068 790069 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 790030 790067 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 790030 790067 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 790028 790029 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 769707 790029 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 769707 769708 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 769688 769706 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 769688 769706 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 769686 769687 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 584302 769687 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 584302 584303 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 584287 584301 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 584287 584301 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 584285 584286 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 584286 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 546039 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 500901 523871 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 500901 500902 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 500896 500900 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 500896 500900 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 500894 500895 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 500895 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 352037 477111 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 352037 352038 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 352028 352036 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 352028 352036 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 352026 352027 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 346231 352027 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 346231 346232 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 346201 346230 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 346201 346230 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 346199 346200 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297980 346200 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297980 297981 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297944 297979 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297944 297979 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297942 297943 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 268497 297943 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 268497 268498 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 268469 268496 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 268469 268496 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 268467 268468 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 193941 268468 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 193941 193942 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 193924 193940 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 193924 193940 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 193922 193923 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 179987 193923 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 179987 179988 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 179947 179986 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 179947 179986 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 179945 179946 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 167021 179946 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 167021 167022 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 167018 167020 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 167018 167020 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 167016 167017 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 118031 167017 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 118031 118032 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 118015 118030 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 118015 118030 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 118013 118014 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 95409 118014 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 95409 95410 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 95343 95408 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 95343 95408 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 95341 95342 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 80860 95342 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 80860 80861 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 80839 80859 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 80839 80859 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 80837 80838 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 61363 80838 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 61363 61364 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 61340 61362 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 61340 61362 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 61338 61339 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 52495 61339 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 52495 52496 . - . intron_id 20 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 52465 52494 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 52465 52494 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 52463 52464 . - . intron_id 22 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 52464 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 23 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 18578 41948 . - . intron_id 23 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 18578 18579 . - . intron_id 22 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 18529 18577 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 18529 18577 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 18527 18528 . - . intron_id 24 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 13438 18528 . - . intron_id 24 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 13438 13439 . - . intron_id 23 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 13415 13437 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 13415 13437 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 13415 1160072 110 - . alignment_id 31 ; Query SPP00000007_1.0 ; Align 1160073 5 18 ; Align 1047028 12 36 ; Align 790068 24 36 ; Align 769706 37 18 ; Align 584302 43 15 ; Align 546049 48 9 ; Align 500901 51 3 ; Align 477123 53 9 ; Align 352036 57 6 ; Align 346230 60 27 ; Align 297979 70 33 ; Align 268496 82 27 ; Align 193941 91 15 ; Align 179986 97 39 ; Align 167021 110 3 ; Align 118031 111 15 ; Align 95407 117 63 ; Align 80858 139 18 ; Align 61361 146 21 ; Align 52495 153 30 ; Align 18578 163 48 ; Align 13436 180 21 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 106 Query range: 12 -> 195 Target range: 1166712 -> 381306 13 : AlaLeuLeuCysGlyAlaLeu{Al} >>>> Target Intron 1 >>>> {a : 20 .!!:!!||||||||| !:!!{ } 862 bp {! ThrValLeuCysGlyArgVal{Ar}+- ++{g 1166712 : ACTGTTCTGTGTGGCCGGGTC{AG}gg.........................ag{A : 1165827 21 : }AlaProGlyLeuAlaGlyThrMetCysAla{S} >>>> Target Intron 2 : 31 }:!!||||||! !|||..! ! ! ! |||{|} 80255 bp }SerProGlySerAlaSerAspGlyArgAla{S}++ 1165826 : }TCTCCCGGCTCGGCCTCCGATGGGCGGGCA{A}gt.................... : 1165793 32 : >>>> {er}ArgAspAspTrpArgCysAlaArgSerMetHisGluPheSerAla : 46 {||}!.!!!: ! !!|||.!! !.!!:!!!!.|||.!.! ! ! ++{er}AsnGluThrAlaGlyCysThrAlaGlyValGlnGluIleMetLys 1165792 : .....ag{GT}AATGAAACCGCAGGATGCACGGCCGGAGTGCAGGAAATAATGAAG : 1085496 47 : LysAspIleAspGlyHisMetValAsnLeuAspLysTyrArgGlyPheValCysIl : 65 ..! ! ! !! !|||! ! !...:!!! ! ||| !!!.! !||||| SerIleGluHisProHisLysCysGlyIleAlaHisTyrGlyAlaArgProCysIl 1085495 : TCGATTGAACATCCCCACAAGTGCGGAATTGCACATTACGGCGCGAGGCCCTGCAT : 1085439 66 : eValThrAsnValAla{Se} >>>> Target Intron 3 >>>> {r}GlnU : 73 |:!! !::::!!|||{ !} 20586 bp {!}!:: eLeuLeuSerLeuAla{Pr}++ ++{o}ArgP 1085438 : CCTCCTGTCATTGGCC{CC}gt.........................ag{G}CGTT : 1064829 74 : nkGlyLysThrGluValAsnTyrThrGlnLeuVal{A} >>>> Target Intr : 85 !.! !:!!:!!.!. ! !:!!|||:!!{|} 42092 b heAlaCysPheGlnIleGlnGlnGluGluLeuLeu{A}++ 1064828 : TCGCTTGTTTCCAGATTCAGCAGGAGGAGCTGCTC{G}gt................ : 1064790 86 : on 4 >>>> {sp}LeuHisAlaArgTyrAlaGluCysGlyLeuArgIleLeuAl : 99 p {||} !||| !! ! !.!||| !||||||||| !! !:! -+{sp}ThrHisPheLeuGlyValGluValGlyLeuArgAlaProSe 1064789 : .........tg{AC}ACTCATTTCCTGGGGGTGGAAGTCGGGCTGCGAGCTCCCTC : 1022659 100 : aPhe{Pr} >>>> Target Intron 5 >>>> {o}CysAsnGlnPheGly : 107 !|||{||} 20657 bp {|} !||| !||| rPhe{Pr}-+ ++{o}IleAsnCysHisGly+ 1022658 : ATTC{CC}tt.........................ag{G}ATTAACTGTCATGGGg : 1001978 108 : >>>> Target Intron 6 >>>> LysGlnGluProGlySerAsnGluGluI : 116 14690 bp ...!!. ! !! !|||!:!|||:!!| + ++AsnHisThrSerLeuSerSerGluLysI 1001977 : t.........................agaaCCATACATCATTGTCAAGCGAGAAGA : 987261 117 : leLysGluPheAlaAlaGlyTyrAsnValLysPheAsp >>>> Target Intr : 129 ||||| .!!!.!:!! !! ||| !:!!.!! ! 10464 b leLysLeuIleValSerPheLeuAsnArgGluLeuLys++ 987260 : TTAAACTTATTGTTTCTTTCTTGAACCGGGAACTCAAGgt................ : 987220 130 : on 7 >>>> >>>> Target Intron 8 >>>> MetPheSerLysIleC : 134 p 35994 bp |||||| |||| ++++ ++TrpPheSerProIleC 987219 : .........aggt.........................agtggttttccccaatCT : 940749 135 : ysVal >>>> Target Intron 9 >>>> AsnGlyAspAspAlaHisProL : 143 || ! 79381 bp !||| ! ! !... !: ysLys+- ++AlaGlyMetGlyGluArgArgV 940748 : GCAAGgg.........................agGCTGGCATGGGGGAGAGAAGAG : 861341 144 : euTrp{Ly} >>>> Target Intron 10 >>>> {s}TrpMetLysIleGl : 150 !!|||{!:} 5643 bp {!}|||:!!!:! !: alTrp{Ar}++ +-{g}TrpLeuArgArgAr 861340 : TGTGG{AG}gt..........................aa{G}TGGCTGAGGCGGCG : 855677 151 : nProLysGlyLysGlyIleLeu{Gl} >>>> Target Intron 11 >>>> : 158 :|||:::|||!:! !:!!! !{||} 4695 bp gProArgGlyArgLeuLeuPro{Gl}+- ++ 855676 : TCCACGTGGGAGGCTCCTACCT{GG}gg..........................ag : 850958 159 : {y}AsnAlaIleLysTrpAsnPheThr{L} >>>> Target Intron 12 >> : 167 {|}||||||:!! !::|||||| { } 179965 bp {y}AsnAlaLeuAlaPheAsnPhePro{G}++ 850957 : {A}AATGCTCTTGCCTTtaacttcccc{g}gt........................ : 850928 168 : >> {ys}PheLeuIleAspLys{A} >>>> Target Intron 13 >>>> : 173 { }|||||| !!!: ! {.} 132705 bp ++{ly}PheLeuAlaGluAsp{G}++ ++ 850927 : ..ag{GC}TTCCTGGCTGAGGAT{G}gt..........................ag : 538244 174 : {sn}GlyCysValValLysArgTyrGly >>>> Target Intron 14 >>>> : 182 {.!}|||||| !||||||!:! !..! 14345 bp {ly}GlyCysTyrValLysLysIleSer-+ 538243 : {GC}GGCTGTTACGTAAAGAAAATCTCGat.......................... : 538214 183 : >>>> Target Intron 15 >>>> ProMetGluGluProLeuValIle : 189 31108 bp !:!!:!! |||:!!||| ! ++++ ++ValValLysProProValValArg 538213 : aggt..........................agGTTGTGAAGCCCCCTGTGGTCCGA : 492742 190 : Glu{L} >>>> Target Intron 16 >>>> {ys}AspLeuProHis : 195 |||{ } 111415 bp { !}|||||||||||| Glu{T}++ ++{rp}AspLeuProHis 492741 : GAG{T}gt..........................ag{gg}gatcttccccac : 381307 vulgar: SPP00000007_1.0 12 195 . NIVO01030317.1:subseq(555646,1182752) 1166712 381306 - 106 M 7 21 S 0 2 5 0 2 I 0 858 3 0 2 S 1 1 M 10 30 S 0 1 5 0 2 I 0 80251 3 0 2 S 1 2 M 39 117 S 0 2 5 0 2 I 0 20582 3 0 2 S 1 1 M 13 39 S 0 1 5 0 2 I 0 42088 3 0 2 S 1 2 M 15 45 S 0 2 5 0 2 I 0 20653 3 0 2 S 1 1 M 5 15 5 0 2 I 0 14686 3 0 2 M 22 66 5 0 2 I 0 10460 3 0 2 5 0 2 I 0 35990 3 0 2 M 7 21 5 0 2 I 0 79377 3 0 2 M 9 27 S 0 2 5 0 2 I 0 5639 3 0 2 S 1 1 M 12 36 S 0 2 5 0 2 I 0 4691 3 0 2 S 1 1 M 8 24 S 0 1 5 0 2 I 0 179961 3 0 2 S 1 2 M 5 15 S 0 1 5 0 2 I 0 132701 3 0 2 S 1 2 M 8 24 5 0 2 I 0 14341 3 0 2 5 0 2 I 0 31104 3 0 2 M 9 27 S 0 1 5 0 2 I 0 111411 3 0 2 S 1 2 M 4 12 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 381307 1166712 106 - . gene_id 32 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1166690 1166712 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1166690 1166712 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1166688 1166689 . - . intron_id 1 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1165828 1166689 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1165828 1165829 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1165796 1165827 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1165796 1165827 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1165794 1165795 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1085541 1165795 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1085541 1085542 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1085420 1085540 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1085420 1085540 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1085418 1085419 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1064834 1085419 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1064834 1064835 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1064793 1064833 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1064793 1064833 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1064791 1064792 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1022701 1064792 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1022701 1022702 . - . intron_id 3 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1022652 1022700 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1022652 1022700 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1022650 1022651 . - . intron_id 5 ; splice_site "TT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1001995 1022651 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1001995 1001996 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1001979 1001994 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1001979 1001994 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1001977 1001978 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 987289 1001978 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 987289 987290 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 987223 987288 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 987223 987288 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 987221 987222 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 987222 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 940765 976758 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 940765 940766 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 940744 940764 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 940744 940764 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 940742 940743 . - . intron_id 9 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 861363 940743 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 861363 861364 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 861334 861362 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 861334 861362 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 861332 861333 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 855691 861333 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 855691 855692 . - . intron_id 9 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 855652 855690 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 855652 855690 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 855650 855651 . - . intron_id 11 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 850957 855651 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 850957 850958 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 850931 850956 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 850931 850956 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 850929 850930 . - . intron_id 12 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 670966 850930 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 670966 670967 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 670948 670965 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 670948 670965 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 670946 670947 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 538243 670947 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 538243 538244 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 538217 538242 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 538217 538242 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 538215 538216 . - . intron_id 14 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 538216 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 492764 523871 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 492764 492765 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 492736 492763 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 492736 492763 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 492734 492735 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 381321 492735 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 381321 381322 . - . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 381307 381320 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 381307 381320 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 381307 1166712 106 - . alignment_id 32 ; Query SPP00000007_1.0 ; Align 1166713 13 21 ; Align 1165827 21 30 ; Align 1085539 32 117 ; Align 1064833 72 39 ; Align 1022699 86 45 ; Align 1001994 102 15 ; Align 987289 107 66 ; Align 940765 129 21 ; Align 861363 136 27 ; Align 855690 146 36 ; Align 850956 159 24 ; Align 670964 168 15 ; Align 538241 174 24 ; Align 492764 182 27 ; Align 381319 192 12 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 105 Query range: 15 -> 192 Target range: 1168584 -> 11205 16 : CysGlyAlaLeuAlaAlaProGlyLeuAlaGlyThrMetCysAlaSerArg{As} : 33 ||||||! ! ! !|||! !! !! !||||||.!!:!!||| !.!!..!{ } CysGlyAspArgArgAlaGlnAspTrpAlaGlyAlaLeuCysTyrGlyGlu{Th}+ 1168584 : TGTGGAGACAGGAGGGCTCAGGACTGGGCAGGAGCCCTGTGTTATGGGGAG{AC}g : 1168531 34 : >>>> Target Intron 1 >>>> {p}AspTrpArg{C} >>>> Target : 37 38811 bp { }||| !!|||{|} 64 + ++{r}AspArgArg{C}++ 1168530 : t.........................ag{G}GACAGGCGC{T}gt........... : 1129707 38 : Intron 2 >>>> {ys}AlaArgSerMetHisGluPheSerAlaLysAspIle : 49 876 bp {||}!.! ! !:!: !..!|||||| !!:!..! ! ++{ys}ValSerLeuValSerArgPheSerArgArgSerCys 1129706 : ..............ag{GC}GTTTCGCTTGTTTCCAGATTCAGCAGGAGGAGCTGC : 1064798 50 : Asp >>>> Target Intron 3 >>>> GlyHisMetValAsnLeuAspLys : 58 ... 1233 bp ||| :::...||| Ser++ ++GlySerLysLeuGlyLeuLeuTyr 1064797 : TCGgt.........................agggatcgaaactgggtctcctgtat : 1063538 59 : TyrArg >>>> Target Intron 4 >>>> GlyPheValCysIleValThr : 67 :::::: 56195 bp ||| |||||| ... TrpGln++ ++GlyAsnValCysAlaCysVal 1063537 : tggcaggt.........................agggaaacgtgtgtgcatgtgta : 1007316 68 : Asn{V} >>>> Target Intron 5 >>>> {al}AlaSerGlnUnkGlyLy : 75 ...{|} 30509 bp {||}||| ! ...|||!. Glu{V}++ ++{al}AlaLeuValSerGlySe 1007315 : gaa{g}gt.........................ag{tg}GCTCTGGTTTCAGGAAG : 976783 76 : sThrGluValAsnTyrThrGln{L} >>>> Target Intron 6 >>>> {e : 83 . !.!.:!! !!:!:!! !{ } 55693 bp { rPheHisLeuProPheSerAla{G}++ ++{l 976782 : TTTCCACCTCCCCTTTTCTGCA{G}gt.........................ag{A : 921065 84 : u}ValAspLeuHisAlaArgTyrAlaGluCysGlyLeu{Ar} >>>> Target : 96 !}:!!...|||!..|||! ! .!!... ||||||{||} 765 u}LeuSerLeuArgAlaLeuProThrArgLeuGlyLeu{Ar}++ 921064 : A}CTGTCACTTCGGGCACTGCCAACGCGCCTGGGACTC{AG}gt............ : 921023 97 : Intron 7 >>>> {g}IleLeuAlaPheProCysAsnGlnPhe >>>> Targ : 106 74 bp {|} ::: :::||| |||!!. ++{g}GlnIleLeuTyrProGlnAsnHisGln++ 921022 : .............ag{g}cagattctttacccgcagAACCACCAGgt......... : 844421 107 : et Intron 8 >>>> GlyLys >>>> Target Intron 9 >>>> Gln : 108 74717 bp ||| 51298 bp ||| ++GlyGly++ ++Gln 844420 : ................agggaggggt.........................agCAG : 718402 109 : Glu{P} >>>> Target Intron 10 >>>> {ro}GlySerAsn{G} >> : 114 |||{ } 115599 bp { !}||||||:::{|} Glu{G}++ ++{ly}GlySerAsp{G}++ 718401 : GAA{G}gt..........................ag{gt}ggctcggat{g}gt.. : 602782 115 : >> Target Intron 11 >>>> {lu} >>>> Target Intron 12 >> : 115 25056 bp {||} 31678 bp ++{lu}++ 602781 : ........................ag{AG}gt........................ : 577724 116 : >> GluIleLys >>>> Target Intron 13 >>>> >>>> Target : 118 |||:!::!! 22168 bp 6 ++GluLeuGln++ ++++ 577723 : ..agGAGCTGCAGgt..........................aggt........... : 523869 119 : Intron 14 >>>> GluPheAlaAlaGlyTyr{A} >>>> Target Intr : 124 749 bp :!!.!!:!!|||.!!:!!{:} 45596 b ++LysIleSerAlaSerHis{A}+- 523868 : ...............agaAGATCTCTGCTAGTCAT{G}gg................ : 517101 125 : on 15 >>>> {sn}ValLysPheAspMetPheSerLys >>>> Target In : 133 p {!!}|||!:!.!!..! |||:!! 17026 -+{sp}ValArgLeuSerSerAlaSerGln++ 517100 : ..........tg{AC}GTCAGACTCTCCTCTGCATCGCAGgt.............. : 471479 134 : tron 16 >>>> IleCysValAsnGlyAspAspAlaHisPro{L} >>>> Ta : 143 0 bp ||||||:!!|||..!..!..!:!! |||{:} ++IleCysLeuAsnSerSerSerSerAlaPro{I}++ 471478 : ............agATCTGTCTAAACTCCTCTTCCTCAGCACCC{A}gt....... : 301188 144 : rget Intron 17 >>>> {eu}TrpLysTrpMetLysIleGln >>>> Tar : 151 15517 bp {!!}||| !:::!: ! :!:||| ++{le}TrpAlaPheLeuHisLeuGln-- 301187 : ...................ag{TC}TGGGCTTTCCTTCACCTGCAGaa........ : 285648 152 : get Intron 18 >>>> ProLysGlyLys{G} >>>> Target Intron : 155 38103 bp |||:::||||||{ } 36049 bp ++ProGlnGlyLys{T}++ 285647 : ..................agcctcagggaaaG{T}gt................... : 247532 156 : 19 >>>> {ly}IleLeu{G} >>>> Target Intron 20 >>>> {ly} : 158 {!!}!!:|||{|} 845 bp {||} ++{rp}MetLeu{G}++ ++{ly} 247531 : .......ag{GG}ATGCTA{G}gt..........................ag{GG} : 210631 159 : AsnAlaIleLysTrpAsnPheThrLysPheLeuIleAspLysAsn{G} >>>> T : 174 :!! !||| ||||||! ||| ! !!:!!:!!! :!!.!.{ } AspArgIlePheTrpAsn***ThrAspValMetValAlaGlnGln{A}++ 210630 : GATAGAATTTTCTGGAATTAGACAGATGTCATGGTTGCACAACAG{A}gt...... : 210581 175 : arget Intron 21 >>>> {ly}CysValValLys{A} >>>> Target I : 179 104745 bp {!!}|||:::||| { } 9457 ++{rg}CysLeuValLeu{A}++ 210580 : ....................ag{ga}tgtctggttcta{g}gt............. : 105821 180 : ntron 22 >>>> {rg}TyrGlyProMetGluGluProLeuValIleGluLysA : 192 7 bp { !} !|||||| ..!!!:|||! ! !:!:.....!| ++{la}AlaGlyProAlaArgAspProArgGlnLeuArgSerA 105820 : .............ag{CA}GCCGGTCCTGCTCGGGACCCACGGCAGCTGCGCTCAG : 11208 193 : sp : 192 || sp 11207 : AC : 11206 vulgar: SPP00000007_1.0 15 192 . NIVO01030317.1:subseq(555646,1182752) 1168584 11205 - 105 M 17 51 S 0 2 5 0 2 I 0 38807 3 0 2 S 1 1 M 3 9 S 0 1 5 0 2 I 0 64872 3 0 2 S 1 2 M 13 39 5 0 2 I 0 1229 3 0 2 M 10 30 5 0 2 I 0 56191 3 0 2 M 8 24 S 0 1 5 0 2 I 0 30505 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 55689 3 0 2 S 1 2 M 12 36 S 0 2 5 0 2 I 0 76570 3 0 2 S 1 1 M 9 27 5 0 2 I 0 74713 3 0 2 M 2 6 5 0 2 I 0 51294 3 0 2 M 2 6 S 0 1 5 0 2 I 0 115595 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 25052 3 0 2 S 1 2 5 0 2 I 0 31674 3 0 2 M 3 9 5 0 2 I 0 22164 3 0 2 5 0 2 I 0 6745 3 0 2 M 6 18 S 0 1 5 0 2 I 0 45592 3 0 2 S 1 2 M 8 24 5 0 2 I 0 170256 3 0 2 M 10 30 S 0 1 5 0 2 I 0 15513 3 0 2 S 1 2 M 7 21 5 0 2 I 0 38099 3 0 2 M 4 12 S 0 1 5 0 2 I 0 36045 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 841 3 0 2 S 1 2 M 15 45 S 0 1 5 0 2 I 0 104741 3 0 2 S 1 2 M 4 12 S 0 1 5 0 2 I 0 94573 3 0 2 S 1 2 M 13 39 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 11206 1168584 105 - . gene_id 33 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1168532 1168584 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1168532 1168584 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1168530 1168531 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1129721 1168531 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1129721 1129722 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1129710 1129720 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1129710 1129720 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1129708 1129709 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1064834 1129709 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1064834 1064835 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1064793 1064833 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1064793 1064833 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1064791 1064792 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1063560 1064792 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1063560 1063561 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1063530 1063559 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1063530 1063559 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1063528 1063529 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1007335 1063529 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1007335 1007336 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1007310 1007334 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1007310 1007334 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1007308 1007309 . - . intron_id 5 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976801 1007309 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976801 976802 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 976759 976800 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 976759 976800 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 921066 976758 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 921066 921067 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 921026 921065 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 921026 921065 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 921024 921025 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 844452 921025 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 844452 844453 . - . intron_id 6 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 844424 844451 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 844424 844451 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 844422 844423 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 769707 844423 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 769707 769708 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 769701 769706 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 769701 769706 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 769699 769700 . - . intron_id 9 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 718403 769700 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 718403 718404 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 718396 718402 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 718396 718402 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 718394 718395 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 602797 718395 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 602797 602798 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 602785 602796 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 602785 602796 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 602783 602784 . - . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 577729 602784 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 577729 577730 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 577727 577728 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 577727 577728 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 577725 577726 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 577726 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 546039 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 517123 523871 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 517123 517124 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 517104 517122 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 517104 517122 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 517102 517103 . - . intron_id 15 ; splice_site "GG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 471508 517103 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 471508 471509 . - . intron_id 14 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 471482 471507 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 471482 471507 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 471480 471481 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 301222 471481 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 301222 301223 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 301191 301221 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 301191 301221 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 301189 301190 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 285674 301190 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 285674 285675 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 285651 285673 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 285651 285673 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 285649 285650 . - . intron_id 18 ; splice_site "AA" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 247548 285650 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 247548 247549 . - . intron_id 17 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 247535 247547 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 247535 247547 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 247533 247534 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 211486 247534 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 211486 211487 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 211477 211485 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 211477 211485 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 211475 211476 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 210632 211476 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 210632 210633 . - . intron_id 19 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 210584 210631 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 210584 210631 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 210582 210583 . - . intron_id 21 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 105839 210583 . - . intron_id 21 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 105839 105840 . - . intron_id 20 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 105824 105838 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 105824 105838 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 105822 105823 . - . intron_id 22 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 11247 105823 . - . intron_id 22 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 11247 11248 . - . intron_id 21 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 11206 11246 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 11206 11246 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 11206 1168584 105 - . alignment_id 33 ; Query SPP00000007_1.0 ; Align 1168585 16 51 ; Align 1129720 34 9 ; Align 1064832 38 39 ; Align 1063560 51 30 ; Align 1007335 61 24 ; Align 976799 70 39 ; Align 921064 84 36 ; Align 844451 97 27 ; Align 769707 106 6 ; Align 718403 108 6 ; Align 602795 111 9 ; Align 546049 115 9 ; Align 517123 118 18 ; Align 471506 125 24 ; Align 301222 133 30 ; Align 285672 144 21 ; Align 247548 151 12 ; Align 211484 156 6 ; Align 210630 159 45 ; Align 105837 175 12 ; Align 11245 180 39 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 103 Query range: 13 -> 195 Target range: 1144443 -> 437959 14 : LeuLeuCysGlyAlaLeu >>>> Target Intron 1 >>>> AlaAlaPro : 22 ||||||||||||.!!||| 56677 bp !.! !||| LeuLeuCysGlyThrLeu++ ++GlyTrpPro 1144443 : CTGCTTTGCGGTACCTTGgt.........................agGGGTGGCCC : 1087742 23 : GlyLeuAlaGlyThrMet >>>> Target Intron 2 >>>> Cys{Al} : 30 ! !:!!:!!||| !! ! 17745 bp |||{::} GluValSerGlyGlnArg++ ++Cys{Se}+- 1087741 : GAGGTCTCAGGGCAGAGGgt.........................agtgc{ag}ga : 1069971 31 : >>>> Target Intron 3 >>>> {a}SerArgAspAspTrpArgCysAlaAr : 39 23502 bp {!}|||||| ! ! ! ||||||::! ! ++{r}SerArgLeuLysSerArgCysSerGl 1069970 : .........................ag{C}TCACGTCTCAAGAGCAGGTGCAGCGG : 1046444 40 : gSerMetHisGluPhe{Se} >>>> Target Intron 4 >>>> {r}AlaL : 47 ! ! !!.|||!:!{||} 18830 bp {|}|||| yValAspGlnGluTyr{Se}++ ++{r}AlaL 1046443 : GGTGGACCAAGAGTAC{AG}gt.........................ag{T}GCAA : 1027590 48 : ysAspIleAspGlyHis{Me} >>>> Target Intron 5 >>>> {t}Val : 54 || ! !.!!|||{:!} 519 bp {:}:!! ysThr***ThrSerHis{Va}++ ++{l}Ile 1027589 : AAACGTGAACCAGCCAC{GT}gt.........................ag{A}ATT : 1027050 55 : AsnLeuAspLysTyrArgGlyPheValCysIleValThrAsnValAlaSerGlnUn : 73 ! :!!||||||!! !.. ...:::||| |||:::::: |||::: IleIleAspLys***AsnArgLeuLeuCysGlnGlnThrSerIle***SerArgLe 1027049 : ATAATAGATAAATAGAacagattactttgccaacaaacgtccatctagtcaaggct : 1026993 74 : kGlyLysThrGluValAsnTyrThrGln{L} >>>> Target Intron 6 >> : 83 ::: :::||| :::{|} 10370 bp uTrpPhePheGln***SerTyrMetGlu{L}++ 1026992 : atggtttttccagtagtcatatatggaa{t}gt....................... : 1026960 84 : >> {eu}ValAspLeu{H} >>>> Target Intron 7 >>>> {is}Ala : 88 {||}! !! !:!!{ } 5234 bp { }.!! ++{eu}GlyAlaVal{G}++ ++{ly}Thr 1026959 : ..ag{TA}GGCGCTGTA{G}gt.........................ag{GA}ACC : 1011344 89 : ArgTyrAlaGluCysGlyLeuArgIleLeuAlaPheProCysAsnGln >>>> T : 105 ||| ||| !||| !! !!.!!!:|||::!! ! !|||!..:!! ArgArgAlaProCysThrGlnAsnMetLeuSerSerValCysArgLys-+ 1011343 : AGGAGGGCACCATGCACGCAGAACATGCTCAGCTCTGTTTGCAGGAAGct...... : 1011291 106 : arget Intron 8 >>>> >>>> Target Intron 9 >>>> {P} > : 105 34535 bp 18295 bp { } ++++ ++{G}++ 1011290 : ...................aggt.........................ag{G}gt. : 958460 106 : >>> Target Intron 10 >>>> {he}GlyLysGlnGluProGlySerAsnG : 114 4460 bp { } :::|||||| ... ++{ly}AspLeuProAspProGlyIleLysP 958459 : .........................ag{gg}gatcttcccgacccaggaatcaaac : 953976 115 : luGluIleLysGluPheAla{A} >>>> Target Intron 11 >>>> {la : 121 ... ...|||{!} 156985 bp {.! roArgSerProAlaLeuAla{G}++ ++{ly 953975 : ccaggtctcctgcactggca{g}gt..........................ag{GA : 796969 122 : }GlyTyrAsnValLysPheAspMetPheSerLysIleCys >>>> Target In : 135 }||| ! |||||| !||| ..! |||! !||| 19623 }GlyLysAsnValLeuPheIleGlnLysSerThrIleLys++ 796968 : }GGAAAGAATGTTCTGTTTATACAGAAAAGCACGATAAAGgt.............. : 796926 136 : tron 12 >>>> ValAsnGlyAspAsp{Al} >>>> Target Intron 13 : 140 0 bp :!!!..|||! |||{ } 8436 bp ++LeuArgGlyGlyAsp{Tr}++ 796925 : ............agCTCAGAGGAGGAGAC{TG}gt..................... : 600679 141 : >>>> {a}HisProLeuTrpLysTrpMet{L} >>>> Target Intron 1 : 148 {!}:!!! !! ! !! !|||:!!{ } 6312 bp ++{p}AsnGlnTrpLeuThrTrpLeu{G}++ 600678 : .....ag{G}AACCAGTGGCTGACATGGCTG{G}gt.................... : 592220 149 : 4 >>>> {ys}IleGlnProLysGlyLysGlyIleLeu{G} >>>> Target : 158 { !}|||...|||:::... ...::: {|} 398 ++{ly}IleAspProArgSerProAlaLeuAla{G}++ 592219 : ......ag{gg}atcgatcccaggtctcctgcattggca{g}gt............ : 585878 159 : Intron 15 >>>> {ly}AsnAla{I} >>>> Target Intron 16 >>> : 161 32 bp {||} !|||{:} 18744 bp ++{ly}AlaAla{V}++ 585877 : ..............ag{GA}GCTGCA{G}gt......................... : 546037 162 : > {le}LysTrpAsnPheThrLysPheLeuIleAspLysAsnGly{Cy} >>>> : 175 {!!} |||:!!!:!:!!...! !:!!:!! ! ! !{ } ++{al}CysTrpHisTyrSerSerCysValLeuThrProAlaVal{Il}++ 546036 : .ag{TC}TGCTGGCATTATTCCTCCTGTGTGCTCACCCCTGCGGTG{AT}gt.... : 527250 176 : Target Intron 17 >>>> {s}ValValLys{Ar} >>>> Target In : 179 50129 bp { }:!!|||!!.{!.} 39103 ++{e}LeuValAsn{As}++ 527249 : ......................ag{A}CTGGTGAAC{AA}gt.............. : 477109 180 : tron 18 >>>> {g}TyrGlyProMetGluGluProLeuValIleGluLysAsp : 192 bp {!} !|||||| :!!! |||||| !:!! ! ++{n}ProGlyProProGlnAlaProLeuArgValProAlaThr 477108 : ............ag{C}CCCGGGCCGCCCCAGGCCCCTCTCCGAGTCCCCGCGACA : 437971 193 : LeuProHis : 195 ||||||||| LeuProHis 437970 : CTCCCCCAC : 437960 vulgar: SPP00000007_1.0 13 195 . NIVO01030317.1:subseq(555646,1182752) 1144443 437959 - 103 M 6 18 5 0 2 I 0 56673 3 0 2 M 9 27 5 0 2 I 0 17741 3 0 2 M 1 3 S 0 2 5 0 2 I 0 23498 3 0 2 S 1 1 M 14 42 S 0 2 5 0 2 I 0 18826 3 0 2 S 1 1 M 7 21 S 0 2 5 0 2 I 0 515 3 0 2 S 1 1 M 29 87 S 0 1 5 0 2 I 0 10366 3 0 2 S 1 2 M 3 9 S 0 1 5 0 2 I 0 5230 3 0 2 S 1 2 M 17 51 5 0 2 I 0 34531 3 0 2 5 0 2 I 0 18291 3 0 2 S 0 1 5 0 2 I 0 4456 3 0 2 S 1 2 M 15 45 S 0 1 5 0 2 I 0 156981 3 0 2 S 1 2 M 13 39 5 0 2 I 0 196226 3 0 2 M 5 15 S 0 2 5 0 2 I 0 8432 3 0 2 S 1 1 M 7 21 S 0 1 5 0 2 I 0 6308 3 0 2 S 1 2 M 9 27 S 0 1 5 0 2 I 0 39828 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 18740 3 0 2 S 1 2 M 13 39 S 0 2 5 0 2 I 0 50125 3 0 2 S 1 1 M 3 9 S 0 2 5 0 2 I 0 39099 3 0 2 S 1 1 M 16 48 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 437960 1144443 103 - . gene_id 34 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1144426 1144443 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1144426 1144443 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1144424 1144425 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1087749 1144425 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1087749 1087750 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1087722 1087748 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1087722 1087748 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1087720 1087721 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1069977 1087721 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1069977 1069978 . - . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1069972 1069976 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1069972 1069976 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1069970 1069971 . - . intron_id 3 ; splice_site "ga" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1046470 1069971 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1046470 1046471 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1046425 1046469 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1046425 1046469 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1046423 1046424 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1027595 1046424 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1027595 1027596 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1027571 1027594 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1027571 1027594 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1027569 1027570 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1027052 1027570 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1027052 1027053 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1026963 1027051 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1026963 1027051 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1026961 1026962 . - . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1016593 1026962 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1016593 1016594 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1016581 1016592 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1016581 1016592 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1016579 1016580 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 1011347 1016580 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 1011347 1011348 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1011294 1011346 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1011294 1011346 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1011292 1011293 . - . intron_id 8 ; splice_site "CT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1011293 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 958464 976758 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 958464 958465 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 958463 958463 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 958463 958463 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 958461 958462 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 954003 958462 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 954003 954004 . - . intron_id 9 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 953955 954002 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 953955 954002 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 953953 953954 . - . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 796970 953954 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 796970 796971 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 796929 796969 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 796929 796969 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 796927 796928 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 600699 796928 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 600699 600700 . - . intron_id 11 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 600682 600698 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 600682 600698 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 600680 600681 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 592246 600681 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 592246 592247 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 592223 592245 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 592223 592245 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 592221 592222 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 585911 592222 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 585911 585912 . - . intron_id 13 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 585881 585910 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 585881 585910 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 585879 585880 . - . intron_id 15 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 585880 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 527296 546039 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 527296 527297 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 527253 527295 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 527253 527295 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 527251 527252 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 477124 527252 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 477124 477125 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 477112 477123 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 477112 477123 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 477110 477111 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 438009 477111 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 438009 438010 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 437960 438008 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 437960 438008 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 437960 1144443 103 - . alignment_id 34 ; Query SPP00000007_1.0 ; Align 1144444 14 18 ; Align 1087749 20 27 ; Align 1069977 29 3 ; Align 1046469 31 42 ; Align 1027594 46 21 ; Align 1027051 54 87 ; Align 1016591 84 9 ; Align 1011345 88 51 ; Align 954001 106 45 ; Align 796968 122 39 ; Align 600699 135 15 ; Align 592245 141 21 ; Align 585909 149 27 ; Align 546047 159 6 ; Align 527294 162 39 ; Align 477123 176 9 ; Align 438008 180 48 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 104 Query range: 1 -> 195 Target range: 987227 -> 108348 2 : Ser{Le} >>>> Target Intron 1 >>>> {u}GlyArgLeuCysArgLeuL : 10 |||{ } 10422 bp {!}||| ! !! !||| !! Ser{Se}++ ++{r}GlySerGlyPheArgLysP 987227 : TCA{AG}gt.........................ag{t}gGCTCTGGTTTCAGGAAGT : 976781 11 : euLysProAlaLeuLeuCys{Gl} >>>> Target Intron 2 >>>> {y}Al : 18 !! !||| !!|||!!!|||{ !} 175571 bp {!} heProProProLeuPheCys{Ar}++ ++{g}As 976780 : TTCCACCTCCCCTTTTCTGC{AG}gt.........................ag{g}ga : 801186 19 : aLeuAlaAlaProGlyLeuAlaGlyThrMetCysAlaSerArgAspAspTrpArgCys : 37 ||| |||||||||::: ||| ::: ||| pLeuProAlaProGlyIleLysProThrSerProIleLeuGlnAlaAspCysLeuThr 801185 : tcttcccgccccaggaatcaaacccacatctcccatattgcaggcagactgtttaacG : 801129 38 : >>>> Target Intron 3 >>>> AlaArgSerMetHisGluPheSerAlaLy : 47 8274 bp ||| !!!!:!::!!||| !!!! !|| ++ ++AlaSerCysValTyrGluAlaThrGlnLy 801128 : gt.........................agGCTTCCTGTGTTTATGAGGCCACACAGAA : 792825 48 : s >>>> Target Intron 4 >>>> AspIleAspGly >>>> Target In : 52 | 8706 bp :!!|||... ! 62546 s-+ ++AsnIleSerGln++ 792824 : Gat.........................agaATATTTCACAGgt.............. : 784102 53 : tron 5 >>>> HisMetValAsnLeuAspLysTyrArgGlyPheValCysIleVal : 66 bp |||! !|||! !! !:!! !!.!..!!:!:!! ! !||| ++HisThrProAsnCysValArgCysHisAsnTyrIleHisTyrVal 784101 : ...........agCATACCCCAAACTGCGTAAGGTGTCACAACTATATACATTATGTT : 721516 67 : ThrAsnValAlaSerGlnUnkGlyLysThrGluValAsnTyrThrGlnLeuValAspL : 86 !.!|||..!!.!! !! ! !!:!! !:!! !||||||! ! ! ||| ! !!| AsnAsnThrGlyTyrProArgAspArgArgGlnProAsnTyrMetTyrLeuSerTyrL 721515 : AATAACACAGGGTACCCCAGGGATAGAAGACAGCCCAACTACATGTACCTAAGTTATC : 721456 87 : eu{H} >>>> Target Intron 6 >>>> {is}Ala >>>> Target Int : 89 ||{|} 40975 bp {||}... 1767 b eu{H}++ ++{is}Gly++ 721455 : TA{C}gt.........................ag{at}ggggt............... : 680470 90 : ron 7 >>>> ArgTyrAlaGluCysGlyLeuArgIleLeuAlaPheProCysAsnG : 104 p ! ! !:!!||| !..!! ! !:!:||||||||||||! !:!!! ++LeuLeuSerGluLeuSerProSerLeuLeuAlaPheProSerHisL 680469 : ..........agCTTCTCTCTGAGCTTTCTCCATCTCTGCTTGCCTTTCCTTCTCACC : 678660 105 : lnPheGlyLysGln >>>> Target Intron 8 >>>> GluProGlySerAsn : 113 .!. ! ||| 43179 bp ||||||!:!!!. euLeuThrPheGln++ ++PheProGlyAsnLys 678659 : TCCTGACTTTCCAGgt.........................agtttcctgGGAATAAA : 635454 114 : Glu{G} >>>> Target Intron 9 >>>> {lu}IleLys{Gl} >>>> Ta : 118 :!!{|} 14102 bp {||}||||||{..} Gln{G}++ ++{lu}IleLys{Ar}++ 635453 : CAA{G}gt.........................ag{aa}atcaaa{ag}gt....... : 621333 119 : rget Intron 10 >>>> {u}{P} >>>> Target Intron 11 >>>> { : 119 43607 bp {!}{ } 31678 bp { ++{g}{G}++ ++{ 621332 : ...................ag{A}{G}gt..........................ag{ : 546048 120 : he}AlaAla{G} >>>> Target Intron 12 >>>> {ly}TyrAsnValLys : 126 }||||||{|} 986 bp {||}|||::: !! ! ly}AlaAla{G}++ ++{ly}TyrAspLysMet 546047 : GA}GCTGCA{G}gt..........................ag{gt}tatgacAAGATG : 545042 127 : PheAspMetPheSerLysIleCysValAsnGlyAspAspAlaHisProLeu{T} >> : 144 ! ! ||| !! !:!!! ! !.!...! ! !:!!||| !|||{|} CysProTyrPheProValLeu***GlyGluSerPheLeuSerHisIleLeu{T}++ 545041 : TGTCCGTATTTCCCTGTGTTATAGGGTGAATCCTTCCTTTCACATATTTTA{T}gt.. : 544985 145 : >> Target Intron 13 >>>> {rp}LysTrpMetLysIleGlnProLysGlyL : 154 22238 bp {||}:::||| !:!.!!::! !!::!|||: -+{rp}ArgTrpGlyArgPheArgAlaArgGlyA 544984 : ........................tg{GG}CGCTGGGGTAGATTCAGGGCCCGGGGGC : 522720 155 : ysGlyIleLeuGlyAsnAla >>>> Target Intron 14 >>>> IleLysTr : 163 ::|||:!:|||||| !!.! 74870 bp !:::|| rgGlyLeuLeuGlyAlaGly++ ++GlyArgTr 522719 : GCGGACTGCTGGGCGCCGGGgt..........................agGGACGCTG : 447823 164 : pAsnPheThr{Ly} >>>> Target Intron 15 >>>> {s}PheLeuIleAs : 171 |||||||:!!{||} 18587 bp {|} !|||::: pAsnPheSer{Ly}++ ++{s}ArgLeuValPr 447822 : GAATTTCTCG{AA}gt..........................ag{A}CGTCtggtccc : 429212 172 : pLysAsnGlyCysVal{V} >>>> Target Intron 16 >>>> {al}LysAr : 179 ::: ||| ..!{|} 112679 bp {||} || oArgAlaGlyValAla{V}++ ++{al}CysAr 429211 : cagggctggggtggcG{G}gt..........................ag{TA}TGCAG : 316509 180 : gTyrGlyPro{Me} >>>> Target Intron 17 >>>> {t}Glu >>>> T : 185 | .!!|||{! } 119317 bp {!}||| gGlySerPro{Ar}++ ++{g}Glu++ 316508 : AGGAAGTCCC{AG}gt..........................ag{G}GAGgt...... : 197172 186 : arget Intron 18 >>>> GluProLeu >>>> Target Intron 19 >>> : 188 74831 bp ||||||:!! 13962 bp ++GluProVal++ 197171 : ....................agGAGCCGGTGgt......................... : 122332 189 : > ValIleGluLysAspLeuProHis : 195 :::... |||||||||||| ++GlyLeuAsnProAspLeuProHis 122331 : .agggattgaacccagatcttccgcAT : 108349 vulgar: SPP00000007_1.0 1 195 . NIVO01030317.1:subseq(555646,1182752) 987227 108348 - 104 M 1 3 S 0 2 5 0 2 I 0 10418 3 0 2 S 1 1 M 13 39 S 0 2 5 0 2 I 0 175567 3 0 2 S 1 1 M 20 60 5 0 2 I 0 8270 3 0 2 M 10 30 5 0 2 I 0 8702 3 0 2 M 4 12 5 0 2 I 0 62542 3 0 2 M 35 105 S 0 1 5 0 2 I 0 40971 3 0 2 S 1 2 M 1 3 5 0 2 I 0 1763 3 0 2 M 20 60 5 0 2 I 0 43175 3 0 2 M 6 18 S 0 1 5 0 2 I 0 14098 3 0 2 S 1 2 M 2 6 S 0 2 5 0 2 I 0 43603 3 0 2 S 1 2 5 0 2 I 0 31674 3 0 2 S 1 2 M 2 6 S 0 1 5 0 2 I 0 982 3 0 2 S 1 2 M 21 63 S 0 1 5 0 2 I 0 22234 3 0 2 S 1 2 M 16 48 5 0 2 I 0 74866 3 0 2 M 6 18 S 0 2 5 0 2 I 0 18583 3 0 2 S 1 1 M 9 27 S 0 1 5 0 2 I 0 112675 3 0 2 S 1 2 M 5 15 S 0 2 5 0 2 I 0 119313 3 0 2 S 1 1 M 1 3 5 0 2 I 0 74827 3 0 2 M 3 9 5 0 2 I 0 13958 3 0 2 M 8 24 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 108349 987227 104 - . gene_id 35 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 987223 987227 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 987223 987227 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 987221 987222 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976801 987222 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976801 976802 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 976759 976800 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 976759 976800 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 801188 976758 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 801188 801189 . - . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 801127 801187 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 801127 801187 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 801125 801126 . - . intron_id 3 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 792853 801126 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 792853 792854 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 792823 792852 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 792823 792852 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 792821 792822 . - . intron_id 4 ; splice_site "AT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 784117 792822 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 784117 784118 . - . intron_id 3 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 784105 784116 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 784105 784116 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 784103 784104 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 721559 784104 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 721559 721560 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 721453 721558 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 721453 721558 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 721451 721452 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 680478 721452 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 680478 680479 . - . intron_id 5 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 680473 680477 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 680473 680477 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 680471 680472 . - . intron_id 7 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 678706 680472 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 678706 678707 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 678646 678705 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 678646 678705 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 678644 678645 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 635467 678645 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 635467 635468 . - . intron_id 7 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 635448 635466 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 635448 635466 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 635446 635447 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 621346 635447 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 621346 621347 . - . intron_id 8 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 621336 621345 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 621336 621345 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 621334 621335 . - . intron_id 10 ; splice_site "gT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 577729 621335 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 577729 577730 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 577727 577728 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 577727 577728 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 577725 577726 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 577726 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 545054 546039 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 545054 545055 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 544988 545053 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 544988 545053 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 544986 544987 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 522750 544987 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 522750 522751 . - . intron_id 12 ; splice_site "TG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 522700 522749 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 522700 522749 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 522698 522699 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 447830 522699 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 447830 447831 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 447810 447829 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 447810 447829 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 447808 447809 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 429223 447809 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 429223 429224 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 429194 429222 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 429194 429222 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 429192 429193 . - . intron_id 16 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 316515 429193 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 316515 316516 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 316496 316514 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 316496 316514 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 316494 316495 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 197179 316495 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 197179 197180 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 197175 197178 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 197175 197178 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 197173 197174 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 122344 197174 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 122344 122345 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 122335 122343 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 122335 122343 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 122333 122334 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 108373 122334 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 108373 108374 . - . intron_id 18 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 108349 108372 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 108349 108372 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 108349 987227 104 - . alignment_id 35 ; Query SPP00000007_1.0 ; Align 987228 2 3 ; Align 976800 4 39 ; Align 801187 18 60 ; Align 792853 38 30 ; Align 784117 48 12 ; Align 721559 52 105 ; Align 680476 88 3 ; Align 678706 89 60 ; Align 635467 109 18 ; Align 621344 116 6 ; Align 546047 120 6 ; Align 545052 123 63 ; Align 522748 145 48 ; Align 447830 161 18 ; Align 429222 168 27 ; Align 316513 178 15 ; Align 197178 184 3 ; Align 122344 185 9 ; Align 108373 188 24 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 101 Query range: 10 -> 195 Target range: 1083462 -> 75640 11 : LysProAlaLeuLeuCysGlyAlaLeuAlaAla >>>> Target Intron 1 : 22 :!!||| !||||||||| !!||| !!.!||| 106671 bp GluProGlnLeuLeuCys***AlaAlaValAla++ 1083462 : GAACCGCAGCTGCTGTGCTGAGCGGCCGTGGCAgt..................... : 1083427 23 : >>>> >>>> Target Intron 2 >>>> ProGlyLeuAlaGlyThrMet : 28 121664 bp ||||||...|||::: ++++ ++AspGlyLeuValGlySerPro 1083426 : ....aggt.........................aggatggactggttggatctcct : 855076 29 : CysAlaSerArgAspAspTrpArg >>>> Target Intron 3 >>>> Cys : 37 |||...|||||||||... ||| 133329 bp ... CysCysSerArgAspSerGlnArg++ ++Ala 855075 : tgctgttcaagggactctcaaagagt.........................aggcg : 721720 38 : AlaArgSerMetHisGluPheSerAlaLysAspIle{A} >>>> Target Int : 50 ||| |||::: ||| ...||| {!} 143952 AlaSerSerLeuAlaGluValProGlyLysProAla{G}++ 721719 : gcttcttcactagctgaggtaccagggaagcctgca{g}gt............... : 721678 51 : ron 4 >>>> {sp} >>>> Target Intron 5 >>>> GlyHisMetVa : 54 bp {!:} 25331 bp ||| ++{lu}++ ++GlyTrpAsnPr 721677 : ..........ag{AG}gt.........................agggatggaaccc : 552386 55 : lAsnLeuAspLysTyrArg >>>> Target Intron 6 >>>> >>>> T : 61 ...||| ::::::::: 28494 bp oArgLeuProArgTrpGln++ ++++ 552385 : gcgtctcccgcgttggcaggt.........................aggt...... : 523869 62 : arget Intron 7 >>>> GlyPheValCysIleVal{T} >>>> Target : 67 52371 bp !.!!:!!||||||!.!{ } 663 ++ThrLeuLeuCysIleAla{G}++ 523868 : ...................agACTCTCCTCTGCATCGCA{G}gt............ : 471479 68 : Intron 8 >>>> {hr}AsnValAlaSerGlnUnkGly >>>> Target In : 75 82 bp { !}:!!|||.!!||| ! ||| 109643 ++{ly}AspValThrSerValValGly++ 471478 : .............ag{GA}GATGTAACATCTGTGGTGGGGgt.............. : 405074 76 : tron 9 >>>> LysThrGluValAsnTyrThrGlnLeuValAspLeuHis >> : 88 bp :!!.!!:!!|||.!. ||||||||| ! !! ! ++GlnAlaGlnValGluLeuThrGlnLeuProIleCysAsp++ 405073 : ...........agCAGGCTCAGGTGGAGCTGACCCAGCTGCCCATCTGTGatgt.. : 295392 89 : >> Target Intron 10 >>>> AlaArgTyrAlaGluCysGlyLeuArgIle : 97 28473 bp |||!.!!! ..! ! !|||||| ! ++AlaHis***CysProThrTyrLeuArgAla 295391 : ........................agGCACATTAGTGTCCCACCTACCTCAGGGCC : 266894 98 : LeuAlaPheProCysAsnGlnPheGlyLysGlnGluProGly{S} >>>> Targ : 112 ||| !!!.||||||::!! ! !||| !! ...|||..!{|} LeuHisLeuProCysSerLeuProGlyAlaProSerProSer{S}++ 266893 : TTGCACTTGCCATGCTCTCTGCCTGGAGCACCCAGCCCTTCT{T}gt......... : 266846 113 : et Intron 11 >>>> {er}AsnGluGluIleLysGluPhe{A} >>>> Ta : 120 26091 bp {||}... |||||||||||| !{!} ++{er}GlyProGluIleLysGluAsp{G}++ 266845 : .................ag{CT}GGGCCTGAGATCAAGGAAGAC{G}gt....... : 240731 121 : rget Intron 12 >>>> {la}AlaGlyTyrAsnValLys >>>> Target : 127 28001 bp { !}|||||| ...|||::: 3 ++{lu}AlaGlyGluGlyValGlu++ 240730 : ...................ag{ag}gctggggagggggtggaggt........... : 212710 128 : Intron 13 >>>> PheAspMetPheSerLysIleCysValAsnGlyAspAsp : 139 367 bp :!:|||..!:!! !.....!!...!! !!..! ++ArgTrpValPheGlnGluAspAlaThrArgSerHisSer 212709 : ...............agagATGGGTCTTCCAAGAGGATGCAACTAGAAGCCACTCC : 209309 140 : AlaHisProLeuTrpLysTrpMet{Ly} >>>> Target Intron 14 >>>> : 148 :!!.!. ! !|||!..||| {!.} 36525 bp SerGluGlyArgTrpSerTrpCys{Se}++ 209308 : TCAGAGGGCAGGTGGAGCTGGTGC{AG}gt.......................... : 209278 149 : {s}IleGlnProLys{G} >>>> Target Intron 15 >>>> {ly}Ly : 154 {.}:!:! |||!:!{|} 16612 bp {||}|| ++{r}LeuProProArg{G}++ ++{ly}Ly 209277 : ag{C}CTGCCCCCGAGG{G}gt..........................ag{ga}aa : 156127 155 : s{G} >>>> Target Intron 16 >>>> {ly}IleLeuGlyAsnAlaIle : 161 |{|} 4767 bp {||}:!!|||||| !! ! s{G}++ ++{ly}LeuLeuGlyAlaGlnThr 156126 : a{g}gt..........................ag{GC}CTCCTGGGAGCACAAACA : 151339 162 : LysTrpAsnPheThrLysPheLeuIleAspLysAsnGlyCysValVal >>>> T : 178 ! !!..!!:!!::! ! !! !!... !|||||| ThrProLysLeuSerArgValAlaArgHisSerProGlyCysProGln+- 151338 : ACCCCTAAGCTCTCCCGAGTGGCTAGGCACTCCCCCGGGTGcccccaggg...... : 151286 179 : arget Intron 17 >>>> LysArgTyrGlyProMetGluGlu >>>> Tar : 186 6853 bp |||!:!||| :::::: ++ValArgPheGlyCysLeuGlnGly++ 151285 : ....................agGTTCGCTTTGGCtgcctgcagggggt........ : 144409 187 : get Intron 18 >>>> ProLeuValIleGluLysAspLeuProHis : 195 68741 bp ||||||||| !||| ! !:!! !||| ++ProLeuValArgGluAspThrIleAspHis 144408 : ..................agCCACTGGTCCGTGAGGACACCATTGACCAC : 75641 vulgar: SPP00000007_1.0 10 195 . NIVO01030317.1:subseq(555646,1182752) 1083462 75640 - 101 M 11 33 5 0 2 I 0 106667 3 0 2 5 0 2 I 0 121660 3 0 2 M 15 45 5 0 2 I 0 133325 3 0 2 M 13 39 S 0 1 5 0 2 I 0 143948 3 0 2 S 1 2 5 0 2 I 0 25327 3 0 2 M 10 30 5 0 2 I 0 28490 3 0 2 5 0 2 I 0 52367 3 0 2 M 6 18 S 0 1 5 0 2 I 0 66378 3 0 2 S 1 2 M 7 21 5 0 2 I 0 109639 3 0 2 M 13 39 5 0 2 I 0 28469 3 0 2 M 24 72 S 0 1 5 0 2 I 0 26087 3 0 2 S 1 2 M 7 21 S 0 1 5 0 2 I 0 27997 3 0 2 S 1 2 M 6 18 5 0 2 I 0 3363 3 0 2 M 21 63 S 0 2 5 0 2 I 0 36521 3 0 2 S 1 1 M 4 12 S 0 1 5 0 2 I 0 16608 3 0 2 S 1 2 M 1 3 S 0 1 5 0 2 I 0 4763 3 0 2 S 1 2 M 22 66 5 0 2 I 0 6849 3 0 2 M 8 24 5 0 2 I 0 68737 3 0 2 M 10 30 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 75641 1083462 101 - . gene_id 36 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 1083430 1083462 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 1083430 1083462 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 1083428 1083429 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 976759 1083429 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 976759 976760 . - . intron_id 0 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 976757 976758 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 855095 976758 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 855095 855096 . - . intron_id 1 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 855050 855094 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 855050 855094 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 855048 855049 . - . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 721721 855049 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 721721 721722 . - . intron_id 2 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 721681 721720 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 721681 721720 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 721679 721680 . - . intron_id 4 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 577729 721680 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 577729 577730 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 577727 577728 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 577727 577728 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 577725 577726 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 552396 577726 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 552396 552397 . - . intron_id 4 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 552366 552395 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 552366 552395 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 552364 552365 . - . intron_id 6 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 552365 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 471501 523871 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 471501 471502 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 471482 471500 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 471482 471500 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 471480 471481 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 405100 471481 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 405100 405101 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 405077 405099 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 405077 405099 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 405075 405076 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 295434 405076 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 295434 295435 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 295395 295433 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 295395 295433 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 295393 295394 . - . intron_id 10 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 266922 295394 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 266922 266923 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 266849 266921 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 266849 266921 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 266847 266848 . - . intron_id 11 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 240758 266848 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 240758 240759 . - . intron_id 10 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 240734 240757 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 240734 240757 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 240732 240733 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 212733 240733 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 212733 212734 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 212713 212732 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 212713 212732 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 212711 212712 . - . intron_id 13 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 209346 212712 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 209346 209347 . - . intron_id 12 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 209281 209345 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 209281 209345 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 209279 209280 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 172756 209280 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 172756 172757 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 172742 172755 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 172742 172755 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 172740 172741 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 156130 172741 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 156130 156131 . - . intron_id 14 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 156124 156129 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 156124 156129 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 156122 156123 . - . intron_id 16 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 151357 156123 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 151357 151358 . - . intron_id 15 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 151289 151356 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 151289 151356 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 151287 151288 . - . intron_id 17 ; splice_site "gg" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 144436 151288 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 144436 144437 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 144412 144435 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 144412 144435 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 144410 144411 . - . intron_id 18 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 75671 144411 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 75671 75672 . - . intron_id 17 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 75641 75670 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 75641 75670 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 75641 1083462 101 - . alignment_id 36 ; Query SPP00000007_1.0 ; Align 1083463 11 33 ; Align 855095 22 45 ; Align 721721 37 39 ; Align 552396 51 30 ; Align 471501 61 18 ; Align 405098 68 21 ; Align 295434 75 39 ; Align 266922 88 72 ; Align 240756 113 21 ; Align 212731 121 18 ; Align 209346 127 63 ; Align 172755 149 12 ; Align 156128 154 3 ; Align 151355 156 66 ; Align 144436 178 24 ; Align 75671 186 30 # --- END OF GFF DUMP --- # C4 Alignment: ------------ Query: SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete Target: NIVO01030317.1:subseq(555646,1182752) Ammotragus lervia isolate NAZ scaffold354_len1753023_cov69, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 101 Query range: 3 -> 175 Target range: 784344 -> 31056 4 : GlyArgLeuCys >>>> Target Intron 1 >>>> ArgLeuLeuLysProAl : 13 ||||||||| 71691 bp ||| !! ! ! !! GlyArgLeuGln++ ++ArgCysHisHisThrGl 784344 : GGGAGACTCCAGgt.........................agaggtGCCATCATACTCA : 712626 14 : aLeuLeuCysGly >>>> Target Intron 2 >>>> AlaLeuAlaAlaProG : 23 !||||||||| !! 40958 bp |||||| !!.!|||| nLeuLeuCysArg++ ++AlaLeuArgGlyProG 712625 : GTTACTCTGCAGGgt.........................agGCTCTGCGAGGCCCCG : 671638 24 : lyLeuAla{Gl} >>>> Target Intron 3 >>>> {y}ThrMet{Cy} >> : 29 || !!..{||} 125579 bp {|}!:! { !} lyGlyVal{Gl}++ ++{y}SerCys{Ar}++ 671637 : GCGGCGtc{gg}gt.........................ag{G}AGCTGC{AG}gt.. : 546037 30 : >> Target Intron 4 >>>> {s}AlaSerArg >>>> Target Intron : 33 18142 bp { }::!|||||| 4016 bp ++{g}SerSerArg++ 546036 : .......................ag{G}AGCAGCAGGgt................... : 527885 34 : 5 >>>> >>>> Target Intron 6 >>>> AspAspTrpArgCysAlaArg : 39 117857 bp !||||||..!||| !! ! ++++ ++ProAspTrpGluCysGlnPro 527884 : ......aggt.........................agCCTGACTGGGAGTGCCAGCCT : 405996 40 : SerMetHis{G} >>>> Target Intron 7 >>>> {lu}PheSerAlaLysA : 48 :!! !!{!} 16967 bp { !}.!.!:!:!!||| AlaGlyAsp{V}++ ++{al}MetAsnSerLysA 405995 : GCTGGAGAC{G}gt.........................ag{TA}ATGAACTCAAAAC : 389002 49 : spIleAspGlyHisMetValAsnLeuAsp{Ly} >>>> Target Intron 8 >> : 58 ||| ! !!!.:!! !||| ! ! {::} 87774 bp rgIleThrValGlnValSerAsnThrLys{Ar}++ 389001 : GGATCACAGTCCAGGTGTCAAACACGAAA{CG}gt....................... : 388968 59 : >> {s}Tyr{Ar} >>>> Target Intron 9 >>>> {g}GlyPheValCys : 64 {:} !{!:} 3211 bp {!}||| ! ++{g}Thr{Gl}++ ++{n}GlyArgSerIle 388967 : ..ag{C}ACC{CA}gt.........................ag{G}GGGCGGTCCATA : 297969 65 : IleValThrAsnValAlaSer{Gl} >>>> Target Intron 10 >>>> {n} : 72 !..! !|||..!|||! !{::} 74837 bp {!} AlaThrTrpAsnThrAlaTyr{Ar}++ ++{g} 297968 : GCCACCTGGAACACAGCATAC{AG}gt..........................ag{G} : 223106 73 : UnkGlyLys >>>> Target Intron 11 >>>> ThrGluValAsnTyrThrG : 82 |||||| 36223 bp :::..!!.!.!. ArgGlyLys++ ++SerSerAlaGlnLeuLeuP 223105 : AGAGGAaaagt..........................agtccTCAGCTCAgctgctgc : 186855 83 : lnLeuValAspLeuHisAlaArgTyrAlaGluCysGly >>>> Target Intron : 95 |||||||||..! !!..! ||| 50805 bp roGlnPheProLeuHisAlaGluLeuProArgMetGly++ 186854 : ctcagtttcctctgcacGCTGAGCTGCCCAGGATGGGGgt.................. : 186814 96 : 12 >>>> LeuArgIleLeuAlaPheProCys{As} >>>> Target Intron : 103 ||| ! !||| !!.!.|||! !{||} 572 bp ++LeuSerAlaLeuProLeuProTyr{As}++ 186813 : ........agctcTCTGCTCTTCCTCTGCCCTAC{AA}gt.................. : 135983 104 : 13 >>>> {n}GlnPhe{G} >>>> Target Intron 14 >>>> {ly}Ly : 107 {|}||||||{|} 46301 bp {||}:! ++{n}GlnPhe{G}++ ++{ly}Gl 135982 : ........ag{T}CAGTTT{G}gt..........................ag{GG}GA : 89102 108 : sGlnGluProGlySerAsnGluGluIleLysGluPheAlaAlaGlyTyrAsnValLys : 126 !! ! |||...|||||| ! ! |||! !!.!||| ... ! ! uProValTrpGlnSerThrGluGluAlaValValPheGluValGlyValGlyGlnHis 89101 : GCCCGtgtggcagagcacagaggaggcAGTTGTCTTTGAAGTTGGGGTGGGGCAGCAT : 89045 127 : PheAspMet >>>> Target Intron 15 >>>> PheSerLysIleCysValA : 136 !:: !..! 8350 bp !!||| ||||||:!!. TrpProGln++ ++ValSerValIleCysLeuG 89044 : TGGCCTCAGgt..........................agGTCTCTGTCATTTGCCTAG : 80665 137 : snGlyAspAspAla >>>> Target Intron 16 >>>> HisProLeuTrpLy : 145 ..|||..!|||!.! 16310 bp |||||| !! ! lyGlySerAspGly+- ++HisProThrSerHi 80664 : GAGGGAGTGATGGGgc..........................agCACCCCACTTCTCA : 64328 146 : sTrpMetLys{I} >>>> Target Intron 17 >>>> {le}GlnProLysGl : 153 !:!: !{ } 6648 bp { }!:!|||::!|| sGlnLeuPro{G}++ ++{ly}ArgProArgGl 64327 : TCAGCTACCA{G}gt..........................ag{GG}CGGCCACGGGG : 57656 154 : yLysGlyIleLeuGlyAsnAlaIleLys{T} >>>> Target Intron 18 >>> : 163 |:::||| ! !!.! ! !:!: !{|} 13321 bp yArgGlyAlaLysAlaProArgLeuAla{T}++ 57655 : CCGTGGGGCCAAGGCACCCAGACTGGCG{T}gt......................... : 57623 164 : > {rp}Asn >>>> Target Intron 19 >>>> >>>> Target Intr : 165 {||}:!! 2351 bp 10859 b -+{rp}His++ ++++ 57622 : .cg{GG}CATgt..........................aggt................ : 41946 166 : on 20 >>>> PheThrLysPheLeuIleAspLysAsnGlyCys : 175 p |||! !.!!:!! ! ! ...|||||| ++AlaThrThrLeuIleGlnGlyLeuGlyGlyCys 41945 : ..........aggcgacGACGCTTATCCAGGGTCTTGGAGGCTGC : 31057 vulgar: SPP00000007_1.0 3 175 . NIVO01030317.1:subseq(555646,1182752) 784344 31056 - 101 M 4 12 5 0 2 I 0 71687 3 0 2 M 10 30 5 0 2 I 0 40954 3 0 2 M 8 24 S 0 2 5 0 2 I 0 125575 3 0 2 S 1 1 M 2 6 S 0 2 5 0 2 I 0 18138 3 0 2 S 1 1 M 3 9 5 0 2 I 0 4012 3 0 2 5 0 2 I 0 117853 3 0 2 M 10 30 S 0 1 5 0 2 I 0 16963 3 0 2 S 1 2 M 14 42 S 0 2 5 0 2 I 0 87770 3 0 2 S 1 1 M 1 3 S 0 2 5 0 2 I 0 3207 3 0 2 S 1 1 M 11 33 S 0 2 5 0 2 I 0 74833 3 0 2 S 1 1 M 3 9 5 0 2 I 0 36219 3 0 2 M 19 57 5 0 2 I 0 50801 3 0 2 M 8 24 S 0 2 5 0 2 I 0 568 3 0 2 S 1 1 M 2 6 S 0 1 5 0 2 I 0 46297 3 0 2 S 1 2 M 23 69 5 0 2 I 0 8346 3 0 2 M 11 33 5 0 2 I 0 16306 3 0 2 M 8 24 S 0 1 5 0 2 I 0 6644 3 0 2 S 1 2 M 13 39 S 0 1 5 0 2 I 0 13317 3 0 2 S 1 2 M 1 3 5 0 2 I 0 2347 3 0 2 5 0 2 I 0 10855 3 0 2 M 11 33 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2017-11-18 ##type DNA # # # seqname source feature start end score strand frame attributes # NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local gene 31057 784344 101 - . gene_id 37 ; sequence SPP00000007_1.0 ; gene_orientation + NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 784333 784344 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 784333 784344 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 784331 784332 . - . intron_id 1 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 712642 784332 . - . intron_id 1 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 712642 712643 . - . intron_id 0 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 712612 712641 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 712612 712641 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 712610 712611 . - . intron_id 2 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 671654 712611 . - . intron_id 2 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 671654 671655 . - . intron_id 1 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 671628 671653 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 671628 671653 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 671626 671627 . - . intron_id 3 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 546049 671627 . - . intron_id 3 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 546049 546050 . - . intron_id 2 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 546040 546048 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 546040 546048 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 546038 546039 . - . intron_id 4 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 527898 546039 . - . intron_id 4 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 527898 527899 . - . intron_id 3 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 527888 527897 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 527888 527897 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 527886 527887 . - . intron_id 5 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 523872 527887 . - . intron_id 5 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 523872 523873 . - . intron_id 4 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 523870 523871 . - . intron_id 6 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 406015 523871 . - . intron_id 6 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 406015 406016 . - . intron_id 5 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 405984 406014 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 405984 406014 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 405982 405983 . - . intron_id 7 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 389017 405983 . - . intron_id 7 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 389017 389018 . - . intron_id 6 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 388971 389016 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 388971 389016 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 388969 388970 . - . intron_id 8 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 301197 388970 . - . intron_id 8 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 301197 301198 . - . intron_id 7 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 301191 301196 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 301191 301196 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 301189 301190 . - . intron_id 9 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 297980 301190 . - . intron_id 9 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 297980 297981 . - . intron_id 8 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 297944 297979 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 297944 297979 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 297942 297943 . - . intron_id 10 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 223107 297943 . - . intron_id 10 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 223107 223108 . - . intron_id 9 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 223097 223106 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 223097 223106 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 223095 223096 . - . intron_id 11 ; splice_site "gt" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 186874 223096 . - . intron_id 11 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 186874 186875 . - . intron_id 10 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 186817 186873 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 186817 186873 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 186815 186816 . - . intron_id 12 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 136012 186816 . - . intron_id 12 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 136012 136013 . - . intron_id 11 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 135986 136011 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 135986 136011 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 135984 135985 . - . intron_id 13 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 135414 135985 . - . intron_id 13 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 135414 135415 . - . intron_id 12 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 135406 135413 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 135406 135413 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 135404 135405 . - . intron_id 14 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 89105 135405 . - . intron_id 14 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 89105 89106 . - . intron_id 13 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 89034 89104 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 89034 89104 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 89032 89033 . - . intron_id 15 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 80684 89033 . - . intron_id 15 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 80684 80685 . - . intron_id 14 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 80651 80683 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 80651 80683 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 80649 80650 . - . intron_id 16 ; splice_site "GC" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 64341 80650 . - . intron_id 16 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 64341 64342 . - . intron_id 15 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 64316 64340 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 64316 64340 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 64314 64315 . - . intron_id 17 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 57668 64315 . - . intron_id 17 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 57668 57669 . - . intron_id 16 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 57626 57667 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 57626 57667 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 57624 57625 . - . intron_id 18 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 44305 57625 . - . intron_id 18 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 44305 44306 . - . intron_id 17 ; splice_site "CG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 44300 44304 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 44300 44304 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 44298 44299 . - . intron_id 19 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 41949 44299 . - . intron_id 19 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 41949 41950 . - . intron_id 18 ; splice_site "AG" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice5 41947 41948 . - . intron_id 20 ; splice_site "GT" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local intron 31090 41948 . - . intron_id 20 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local splice3 31090 31091 . - . intron_id 19 ; splice_site "ag" NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local cds 31057 31089 . - . NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local exon 31057 31089 . - . insertions 0 ; deletions 0 NIVO01030317.1:subseq(555646,1182752) exonerate:protein2genome:local similarity 31057 784344 101 - . alignment_id 37 ; Query SPP00000007_1.0 ; Align 784345 4 12 ; Align 712642 8 30 ; Align 671654 18 24 ; Align 546048 27 6 ; Align 527897 30 9 ; Align 406015 33 30 ; Align 389015 44 42 ; Align 301196 59 3 ; Align 297979 61 33 ; Align 223106 73 9 ; Align 186874 76 57 ; Align 136012 95 24 ; Align 135413 104 6 ; Align 89103 107 69 ; Align 80684 130 33 ; Align 64341 141 24 ; Align 57666 150 39 ; Align 44303 164 3 ; Align 31090 165 33 # --- END OF GFF DUMP --- # -- completed exonerate analysis