TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: genome.fa 20,089 sequences; 1,310,797,575 total letters Query= SPP00001058_2.0 # Protein # Selenoprotein S (SELENOS) # Chicken Length=194 Score E Sequences producing significant alignments: (Bits) Value NOIK01000026.1 Anas zonorhyncha breed spot-billed scaffold27, wh... 54.3 2e-13 >NOIK01000026.1 Anas zonorhyncha breed spot-billed scaffold27, whole genome shotgun sequence Length=5302363 Score = 54.3 bits (129), Expect(2) = 2e-13, Method: Compositional matrix adjust. Identities = 26/28 (93%), Positives = 27/28 (96%), Gaps = 0/28 (0%) Frame = -2 Query 140 QQEAESGASTSSAVPKSKPNKKPLRGGG 167 QQE ESGASTS+AVPKSKPNKKPLRGGG Sbjct 4264476 QQEVESGASTSAAVPKSKPNKKPLRGGG 4264393 Score = 43.1 bits (100), Expect(2) = 2e-13, Method: Compositional matrix adjust. Identities = 20/21 (95%), Positives = 20/21 (95%), Gaps = 0/21 (0%) Frame = -1 Query 120 IEMWESMQEGKSYKGNLKLSQ 140 I MWESMQEGKSYKGNLKLSQ Sbjct 4264639 IAMWESMQEGKSYKGNLKLSQ 4264577 Score = 60.8 bits (146), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 32/54 (59%), Positives = 37/54 (69%), Gaps = 0/54 (0%) Frame = -1 Query 20 REGLELLQHTVGALLSSYGWYILLACVAIYLIVQKISPYLRMRPSSQQGATGAA 73 R L L+ VG+LLS+YGWYILL CV IY+IVQKIS L R S + GA AA Sbjct 4266523 RPNLRLVPLAVGSLLSNYGWYILLGCVVIYVIVQKISQSLAARQSGRAGAVDAA 4266362 Score = 53.5 bits (127), Expect = 8e-07, Method: Composition-based stats. Identities = 24/28 (86%), Positives = 27/28 (96%), Gaps = 0/28 (0%) Frame = -1 Query 74 VEPDMVVRRQEALLASRLRMQEELNAQA 101 +EPD+VVRRQEAL A+RLRMQEELNAQA Sbjct 4265251 LEPDLVVRRQEALAAARLRMQEELNAQA 4265168 Score = 36.2 bits (82), Expect = 0.39, Method: Compositional matrix adjust. Identities = 15/15 (100%), Positives = 15/15 (100%), Gaps = 0/15 (0%) Frame = -1 Query 167 GYNPLSGEGGGTCSW 181 GYNPLSGEGGGTCSW Sbjct 4262437 GYNPLSGEGGGTCSW 4262393 Lambda K H a alpha 0.312 0.129 0.372 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 32590645050 Database: genome.fa Posted date: Oct 23, 2017 5:50 PM Number of letters in database: 1,310,797,575 Number of sequences in database: 20,089 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40