_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 3e-19 Query name: gi|6649223|gb|AAF21431.1|AF166126_1 selenoprotein Zf1 [Homo sapiens] Query range: 1-277 (total 277) Target: /data/www-rg/seblastian_results/293455631/tempfiles/temptarget.fasta Chromosome: KZ294150.1:subseq_2264215,95001_ Strand: - Positions: -Exon 1 49581 49613 -Exon 2 48933 49107 -Exon 3 35088 35224 -Exon 4 31587 31682 -Exon 5 31143 31235 -Exon 6 29135 29206 -Exon 7 28843 28940 -Exon 8 25000 25126 Redox box: no Query MRSIPLRGFDQ <---Intron---> QMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIG <---Intron--- ||||||||||| < 473bp > ||||/|/||| ||||/||/|| |/ //||||||||||||| |/||/||| ||||||||| < 13708bp Target MRSIPLRGFDQ QMSSLVMEHMESHGTQFLKGCIPTCIKRLPDGQLQVTWEDRTSGKKDTGIFDTVLWAIG acaacccgtgc catttgagcagtcgactcagtacataaaccggcccgatggcatgaagagatgagctgag tggtctggtaa atcctttaatacagcattaggtccgtagtcagatatcgaagccgaaacgttacttgct gctcctgctcg agttgcggcggtcccacggtccccccggtatcacgtcggccctcggcacctctcggta Query > RVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVE <---Intron---> GRPELTPTAIMAGRLLVQRLFGGSSDLMDYDN <--- > |/|/||/|||// ||/ /|/ |||/||/// |||||||||||| | < 3405bp > |||||||||||||/|| ||||| |||||||/| < Target RIPETRNLNLKEVGVNINPENQKIIVDAQDVTSVPHIYAIGDVAE GRPELTPTAIMAGKLLAQRLFGQSSDLMDYNN cacgaaacacaggggaaacgacaaagggcggatgccatgaggggg gccgcacagaaggaccgccctgcttgcagtaa ggtcacgatataatgtatacaaaatttacaatcctcatactgatca ggcatccccttcgattcagttgaccattaaaa tataacatgtggatagtactgtagctttcgtcctcccctctatttg ggtggacatcgaggtgcggctaacacggttct Query Intron---> VPTTVFTPLEYGCVGLSEEEAVARHGQEHVE <---Intron---> VYHAHYKPLEFTVAGRDASQCYVK <---Intron---> MV 351bp > |||||||||||||||||||||||||||||/| < 1936bp > ||||/||||||||| |||||||/| < 194bp > || Target VPTTVFTPLEYGCVGLSEEEAVARHGQEHIE VYHAYYKPLEFTVAERDASQCYIK MV gcaagtactgtgtggttggggggccgcgcag gtcgttaccgtagggcggtcttaa ag tcccttcctaaggtgtcaaactcgagaaata taacaaactatctcagaccagata tt tcttcctagatctgggaaggtgtctaagttg ttcattaaagcagtaatacgtcag gg Query CLREPPQLVLGLHFLGPNAGEVTQGFALGIK <---Intron---> CGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCUG ||/|||||||||||||||||||||||||||| < 3716bp > |||||||||||||||||||||||||||||||||||||||||| Target CLKEPPQLVLGLHFLGPNAGEVTQGFALGIK CGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCUG tcagccccgcgcctcgcaggggacgtgcgaa tggttgcgaaaggaccattggggaccatactgcgcagagttg gtaaccatttgtattgcacgatcagtctgta ggccacattgctgtaccgcaattatgtcagcgtacctcgggg cgggcaggggcgccccccttattaattggc acgcctaggggagcccccttgggcgatccgcacgcttgtccat * Sequence until next stop: TAA * Distance CDS-SECIS: 1365 ; UGA-SECIS: 1368 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = KZ294150.1:subseq_2264215,95001_ Strand = - Positions = 23634 - 23561 Found by: Infernal (score=35.63) Covels (score=39.03) Stem2 length = 10 Apical loop length = 16 Insertion in stem2 = 0, 0 Free Energy = -23.89 ..(((((((.......(((((((((((((((................)))))))))))))))........))))))).. model uucggucaugcgUuaAUGAcGgccuggcccuAAAcC-cuuuuuggggcgggccaggcCuGAUGuuuuuucaugaccggc .((((((((.--....([[{((({(((((((................)))))))})))}]])..--..-.)))))))). target UAAAUGCCAA--AUGAUGACGGCCUGGGCAGAAACCCACUCUGUGGGCUGCCCAUGUCCGAGCC--CC-CUGGCGUUUC **** ^^^ ****