________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 5e-05 Query name: gi|379698834|ref|NP_001243891.1| thioredoxin reductase 1, cytoplasmic [Oryctolagus cuniculus] Query range: 1-499 (total 499) Target: /data/www-rg/seblastian_results/790677170/tempfiles/temptarget.fasta Chromosome: KZ294002.1:subseq_1337875,80123_ Strand: - Positions: -Exon 1 58354 58440 -Exon 2 56602 56674 -Exon 3 55003 55122 -Exon 4 50113 50255 -Exon 5 49621 49736 -Exon 6 47526 47751 -Exon 7 38469 38561 -Exon 8 37841 37917 -Exon 9 37067 37223 -Exon 10 31909 32016 -Exon 11 29061 29156 -Exon 12 25003 25137 -Exon 13 13037 13102 Redox box: no Query MNGSEDLPPSYDYDLIIIGGGSGGLAAAK <---Intron---> EAAKYGKKVMVLDFVTPTPLGTRWG <---Intron---> LGGTCVNVGCIPKK |||||||| |||/|||||||||||||||| < 1679bp > ||||| ||||||||||||||||||| < 1479bp > |||||||||||||| Target MNGSEDLPESYDFDLIIIGGGSGGLAAAK EAAKYDKKVMVLDFVTPTPLGTRWG LGGTCVNVGCIPKK aagtggccgttgtgcaaagggtggcggga gggatgaagagtgtgacaccgaatg cggatgaggtacaa tagcaatcacaatattttgggcggtccca accaaaaattttattcccctgcgg gtggcgtatggtcaa gtctgttcgtttcctcctatcaaggttcg gacatcggtgcgttctacttatag ttaagtgtgtcataa Query LMHQAALLGQALQDSRNYGWKFEDTV <---Intron---> EHDWEKMTEAVQNHIGSLNWGYRVALREKKVVYENAYGEFIGPHQIK <---Intron ||||||||||||/||||||| ||| | < 4747bp > /|||||||||||||||||||||||||||||||||||/|/|||||/|| < 376bp Target LMHQAALLGQALKDSRNYGWNFEDNV KHDWEKMTEAVQNHIGSLNWGYRVALREKKVVYENAFGQFIGPHRIK caccggttgcgcagtcatgtatggag acgtgaaagggcacagtcatgtcggccgaaggtgagtgctagccaaa ttaaccttgactaacgaaggataaa taaagaatcactaaatgctaggagtctgaaattaaactgattgcagta agcaatgaaacgactcctcgccgct tatcgaagaatagtctctgcgcctataggaggctgcttgatcattgtg Query ---> ATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISS <---Intron---> DDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMV > ||||||||| |||||||||||||||||||||||||||| < 1869bp > |||||||||||||||||||||||||||||||||||||||| Target ATNNKGKEKFYSAERFLIATGERPRYLGIPGDKEYCISR DDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMV gaaaagagatttggctcagaggaccttgacggagttaaa ggctttcttcgaacggggttggtgtggtcggagtggagag ccaaagaaataccagtttccgagcgatgtcgaaaagtgg aattctcagcgactttgccatctagcgttcgtgtatcttt aatcacaaattaagatctcttagatcgcccgcggcccc attatcatccgtgcggtaactctgattattttttgtcttgg Query RSILLRGFDQDMANKIGAHMEEHGVKFIRQFVPIK <---Intron---> VEQIEAGTPGRLRVVAKSTNSEETIEGEYNT <---Intron---> VL ||||||||||||||||| ||||||/|||/|||||| < 8964bp > /||||||||||||| |/|||||| ||||||| < 551bp > || Target RSILLRGFDQDMANKIGEHMEEHGIKFIKQFVPIK IEQIEAGTPGRLRVTAQSTNSEEIIEGEYNT VL ctaccagtgcgagaaaggcaggcgaataactgcaa agcagggacgccagagctaaaggaagggtaa gc gctttggtaaatcaatgaataaagtattaattcta taatacgccggtgtccaccagaattagaaac tt gttcgaatcgcgctattacgaattcgtaggtaata taataagaacacggatgccttgactaagttg ag Query LAIGRDACTRKIGLETVGVKINEK <---Intron---> TGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYAGSTVK <---Int ||||||/||| ||||||||||||| < 617bp > |||||||||||||||||||||||||||||||||||||||||||||||||||| < 505 Target LAIGRDSCTRNIGLETVGVKINEK TGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYAGSTVK cgagagttaaaagtgagggaaaga agaacgagggcaagctatgaggatggacgcacggacggatcgcactggtaga tctggacgcgatgtactgtataaa cgatctcaaaacatcatactgattagatatcctctacggttcagtacgccta gaaaatttaattcaatggggata atcaatcgtaagctgtcctctttaggaggatcagccgaaaggcggtttcctcg Query ron---> CDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENVE <---Intron---> VYHSFFWPLEWTIPSRDNNRCYAKIVCNLKDN <---Int 0bp > ||||||||||||||||||||||||||||||||||/| < 2752bp > ||||||||||||/||||||/|||||||||||| < 392 Target CDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIE VYHSFFWPLEWTVPSRDNNKCYAKIVCNLKDN tgtgagcaagtactgtggtgctggagggatgggaag gtcatttccgtagctagaaattgaagtacaga gaaaatcccttcctaagcggtcaaactaatgaaata taagttgctagctccgaaaagacattgataaa tctattaatatttgattttctaaggtagatgggctg tcttctgagagataaatccattaaacttgact Query ron---> ERVVGFHVLGPNAGEVTQGFAAALKCGLTKTQLDSTIGIHPVCAE <---Intron---> VFTTLSVTKRSGGDILQAGCUG 3bp > |||||||||||||||||||||||||||||| |||||||||||||| < 11900bp > |||||||||||||||||||||| Target ERVVGFHVLGPNAGEVTQGFAAALKCGLTKRQLDSTIGIHPVCAE VFTTLSVTKRSGGDILQAGCUG gcgggtcgcgcaggggacgtgggcatgcaaaccgaaagaccgtgg gtaactgaactgggaccggttg agttgtattgcacgatcagtccctaggtcagatagctgtactgca ttcctctcagcggattacgggg attgcttagcattaacggccatgcgtagcggggccgtacctctag gcaggggcgctgccctgtccat * Sequence until next stop: TAA * Distance CDS-SECIS: 227 ; UGA-SECIS: 230 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = KZ294002.1:subseq_1337875,80123_ Strand = - Positions = 12809 - 12731 Found by: Infernal (score=27.45) Covels (score=22.57) Stem2 length = 10 Apical loop length = 14 Insertion in stem2 = 1, 0 Free Energy = -13.31 ..(((((((............((((((((((.(((((...............)))))))))))))))........))))))).. model uucggucaugcg-----UuaAUGAcGgccug-gcccuAAAcCcuuuuuggggcgggccaggcCuGAUGuuuuuucaugaccggc ..(((({((............([[[((((((.((((......---........))))))))))]]])..--....))})))).. target UGCAUCAGAGUGAUGCGUCCAUGAAGUCGCCAGCCUCAAGCC---CGAGUGGUGGGCGGUGAUGGAACA--GCUGUCAGAUGAG **** ^^ ****