_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 2e-25 Query name: gi|914930242|ref|XP_005341979.2| PREDICTED: glutathione peroxidase 6 [Ictidomys tridecemlineatus] Query range: 1-221 (total 221) Target: /data/www-rg/seblastian_results/113286254/tempfiles/temptarget.fasta Chromosome: KZ294329.1:subseq_603787,54660_ Strand: - Positions: -Exon 1 32464 32550 -Exon 2 29504 29657 -Exon 3 27173 27290 -Exon 4 26501 26600 -Exon 5 24997 25200 Redox box: no Query MNWQFQATCLFPLFLIGFCQEILKPQMVK <---Intron---> MDCNKGVTGTIYEYGALTLSGEEYIQFKKYAGKHVLFVNVATYUGLTAQYPE <- ||||||||||||||||||||||||||||| < 2806bp > ||||| |||||||||||||||||||||||||||||||||||||||||||||| < Target MNWQFQATCLFPLFLIGFCQEILKPQMVK MDCNKRVTGTIYEYGALTLSGEEYIQFKKYAGKHVLFVNVATYUGLTAQYPE aatctcgatctcttcagttcgacaccaga agtaaagagaatgtggcacagggtactaatggacgctgaggattgtagctcg tagataccgttcttttgtgaattacatta tagaagtcgctaaagctctggaaatataaacgaattttatccaggtccaac gcggcgcttttggcgtcctggcggaaggg gtccaggtccctgtacctcttggccgcagttcgtcctccgcctacgatgtt * Query --Intron---> LNALQEELKHLDVIVLGFPCNQFGKQEPGKNSEILSGLK <---Intron---> YVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFL 2213bp > ||||||||||||||||||||||||||||||||||||||| < 572bp > |||||||||||||||||||||||||||||||| Target LNALQEELKHLDVIVLGFPCNQFGKQEPGKNSEILSGLK YVRPGGGFVPNFQLFEKGDVNGEKEQKVFTFL cagccggcactggagtgtctactgacgcgaatgactgca tgccgggtgcatcctgagggaggagcagtatc atactaaataatattttgtcgaatgaaacgaacattcgta atgcgggttcatattaagatagaaaaattctt agtaggggggtatctggcttttataaaaaaacagtctgc gtgtattctccttgctgattataaaaggctacc Query K <---Intron---> NSCPPTSELLGSSKQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHQIPVSTVKLDILEYLKQFDTQ | < 1300bp > |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Target K NSCPPTSELLGSSKQLFWEPMKVHDIRWNFEKFLVGPDGVPVMRWFHQIPVSTVKLDILEYLKQFDTQ a attccatgctgttaccttgcaagcgactatgatcggcgggcgacttccacgaagatgacgtcactgac a acgccccattgccaattgactataatggataatttgcagtcttggtaatctgctatattaataataca g tcctactatgttaaaccggcggcttccgctggcgggttactcgcgccgtactacagccgacaggttcg Sequence until next stop: TAG * Distance CDS-SECIS: 170 ; UGA-SECIS: 614 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = KZ294329.1:subseq_603787,54660_ Strand = - Positions = 24826 - 24751 Found by: Infernal (score=31.4) Covels (score=22.42) Stem2 length = 10 Apical loop length = 15 Insertion in stem2 = 0, 1 Free Energy = -27.16 ..(((((((.......(((((((((((((((...............))))))).))))))))..........))))))).. model uucggucaugcgUuaAUGAcGgccuggcccuAAAcCcuuuuuggggcgggcca-ggcCuGAUGuu-u-uuucaugaccggc (({(((((((--....([[(((((-((((((...............))))))-.)))))]])......-..)))))))})) target GAGGGGCAUU--UUCAUGAUGGUG-GGUCCGAAAGCCCCUCUGAGCCGGACC-CCACCAGAACCUUCC-UUGGUGCCUGUC **** ^^^ ****