_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 2e-05 Query name: gi|387598071|ref|NP_001248375.1| thioredoxin reductase 1, cytoplasmic isoform 5 [Homo sapiens] Query range: 1-461 (total 461) Target: /data/www-rg/seblastian_results/155653343/tempfiles/temptarget.fasta Chromosome: KV389434.1:subseq_1333459,100236_ Strand: + Positions: -Exon 1 40552 40597 -Exon 2 42925 43044 -Exon 3 46795 46937 -Exon 4 47425 47540 -Exon 5 50005 50230 -Exon 6 53294 53386 -Exon 7 54572 54648 -Exon 8 55315 55471 -Exon 9 55998 56105 -Exon 10 58264 58359 -Exon 11 62298 62432 -Exon 12 72590 72655 Redox box: no Query MVLDFVTPTPLGTRWG <---Intron---> LGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETV <---Intron---> KHDWDRMIEAVQ |||||||||||||||| < 2327bp > |||||||||||||||||||||||||||||||||||||||| < 3750bp > |||||||||||| Target MVLDFVTPTPLGTRWG LGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETV KHDWDRMIEAVQ agcgtgacaccgaatg cggatgaggtacaacaccggttgcgccgtcatgtagggag acgtgaaagggc tttattcccctgcgg gtggcgtatggtcaattaaccttgactaacgaaggataac taaagagttacta gcgctctccttatag tcaaatgtgtcataaggcattgaaacaactactagacgga tgttgcagaatag Query NHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIK <---Intron---> ATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISS <---Intro ||||||||||||||||||||||||||||||||||| < 487bp > ||||||||||||||||||||||||||||||||||||||| < 2464b Target NHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIK ATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISS acagttatgtcggccgaaggtgagtgctagccaaa gaaaagagaattggatcagaggaccttgacggagttaaa aatgctaggagtctgaaattaaacagattgcagta ccaaagaaataccagtttccgagcgatgtcgaaaagtgg tctctgcgccgatggggatctgtttgattttcgtg aattacaagttaagatctcttaaatcgccttcaacccc Query n---> DDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIK <---Intron---> VEQ p > ||||||||||||||/|||||||||||||||||||||||||||||||||||/|||||||||||||||||||||||| < 3063bp > ||| Target DDLFSLPYCPGKTLIVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQEMANKIGEHMEEHGIKFIRQFVPIK VEQ ggctttcttcgaacagggttggtgtggtcggagtggagagataccagtgcgagaaaggcaggcgaataactgcaa ggc aattctcagcgactttgccatctagcgttcgtgtatctttgctttggtaaatcaatgaataaagtattgattcta taa ttttccatccgggccctaactttgattattttttatcttgtgctttaatcgagccattacgaatccgtaagcaata caa Query IEAGTPGRLRVVAQSTNSEEIIEGEYNT <---Intron---> VMLAIGRDACTRKIGLETVGVKINEK <---Intron---> TGKIPVTDEEQTNV |||||||||||||||||||||||||||| < 1185bp > |/|||||||||||||||||||||||| < 666bp > |||||||||||||| Target IEAGTPGRLRVVAQSTNSEEIIEGEYNT VLLAIGRDACTRKIGLETVGVKINEK TGKIPVTDEEQTNV agggacgcccgggctaaaggaagggtaa gttgagaggtaaaagtgagggaaaga agaacgagggcaag tacgccggtgttcaccagaattagaaac tttctggacgcgatgtactgtataaa cgatctcaaaacat taagaacacaaatgtcttgactaagttg gggaacattcaaatcaatagggata gtaaatcataggccg Query PYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVK <---Intron---> CDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIE <---Intro ||||||||||| |||||||||||||||||||||||||| < 526bp > |||||||||||||||||||||||||||||||||||| < 2158b Target PYIYAIGDILEGKVELTPVAIQAGRLLAQRLYAGSTVK CDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIE ctatgaggatggaggcacggacggatcgcactggtaga tgtgagcaagtactgtggtgctggagggatgggaag catactgattagatatcctctacggttcagtacgccta gaaaatcccttcctaagcggtcaaactaatgaaata tcctctctaggtgggtcatacgaaaggtggctatcttg tctattgctatttgatattcttggatgggtgaattg Query n---> VYHSYFWPLEWTIPSRDNNKCYAKIICNTKDN <---Intron---> ERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAE p > |||||||||||||||||||||||||||| ||| < 3938bp > ||||||||||||||||||||||||||||||||||||||||||||| Target VYHSYFWPLEWTIPSRDNNKCYAKIICNIKDN ERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAE gtcatttctgtaactagaaattgaaataaaga gcgggtcgcgcaggggacgtgggcatgcaaaccgaaagaccgtgg taagatgctagctccgaaaagacattgataaa agttgtattgcacgatcagtccctaggtcaaatagctgtactgca tcttctgagaggtaaatccattaaactttact attgctcagtattaataaccatagatagcaaggccatacctctag Query <---Intron---> VFTTLSVTKRSGASILQAGCUG < 10157bp > |||||||||||||||||||||| Target VFTTLSVTKRSGASILQAGCUG gtaactgaactggaaccggttg ttcctctcagcgcgttacgggg acaactgcactgaccggtccat * Sequence until next stop: TAA * Distance CDS-SECIS: 219 ; UGA-SECIS: 222 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = KV389434.1:subseq_1333459,100236_ Strand = + Positions = 72875 - 72961 Found by: Infernal (score=24.19) Covels (score=18.72) Stem2 length = 9 Apical loop length = 14 Insertion in stem2 = 1, 0 Free Energy = -11.19 ..(((((((......................((((((((((.(((((...............)))))))))))))))........))))))).. model uucggucaugcg---------------UuaAUGAcGgccug-gcccuAAAcCcuuuuuggggcgggccaggcCuGAUGuuuuuucaugaccggc .(((((((((.....................([[[((((({.((((......---........))))})))))]]])----...))))))))). target CAUUUGGCAGGGCAUUGUAAGGAUGCAUCCAUGAAGUCACCAGUCUCAAGCC---CAUGUGGUAGACAGUGAUGGAA----UAACUGUCAAAUC **** ^^ ****