_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 1e-92 Query name: gi|1044366702|ref|XP_017388639.1| PREDICTED: selenoprotein P isoform X2 [Cebus capucinus imitator] Query range: 1-381 (total 381) Target: /data/www-rg/seblastian_results/32125515/tempfiles/temptarget.fasta Chromosome: KV389506.1:subseq_2207742,100551_ Strand: - Positions: -Exon 1 56008 56210 -Exon 2 54732 54944 -Exon 3 52489 52606 -Exon 4 49943 50551 Redox box: YES Query MWRSLGLALALCLLPSGGTESQDQSSLCKQPPAWSIRDQDPMLNSTGSVTVVALLQASUYLCILQASR <---Intron---> LEDLRVKLEKEGYSNI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| < 1063bp > |||||||||||||||| Target MWRSLGLALALCLLPSGGTESQDQSSLCKQPPAWSIRDQDPMLNSTGSVTVVALLQASUYLCILQASR LEDLRVKLEKEGYSNI ataacgcgcgctccctggagacgcatttacccgtaaagcgcacatagtgagggcccgattctaccgta tggccgacgaggttaa tgggtgtctctgttccggcagaaagctgaacccggtgaaacttaccgctcttcttacggatgttaccg taatgtataaagacat ggacggtcgtctcccaaaagcgcaccatgacacgcaatataggccttagtgttttactacgctggat agacgaaaggaaatttt * Query SYIIVNHQGLSSQLRYTHLKNQVSEHIPVYQQEENQPDVWALLNGNKDDFLIYDR <---Intron---> CGRLVYHLGLPFSFLTFPYVEEAIKIAYC ||||||||||||||||||||||||||||||||||||||||||||||||||||||| < 2125bp > ||||||||||||||||||||||||||||| Target SYIIVNHQGLSSQLRYTHLKNQVSEHIPVYQQEENQPDVWALLNGNKDDFLIYDR CGRLVYHLGLPFSFLTFPYVEEAIKIAYC ttaagaccgcttctataccaacgtgcacgtccggaccggtgctagaaggtcatga tgccgtccgtctttcatctggggaaagtt catttaaagtccatgacataaatcaatctaaaaaaacatgcttagaaaatttaag gggttaatgtctcttctcataactatcag tttttttaacttaaacattgtgtagtttttgaaacaatcgttatacatcccatc atcttattttgttccaacataaactgttct Query EKKCGNCSLT <---Intron---> TLKDEDFCKNVSLATVNKTTEAPQPHHHHKHHRNHGHQHLGSSELSKNQQPGAPDAPTHLTPSDLHHHHKHKGQ |||||||||| < 1937bp > |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Target EKKCGNCSLT TLKDEDFCKNVSLATVNKTTEAPQPHHHHKHHRNHGHQHLGSSELSKNQQPGAPDAPTHLTPSDLHHHHKHKGQ gaatgattca acagggttaagttgagaaaaggcccccccacccacgccccgaagctaacccggcggcaccactgcccccacagc aaaggagctc ctaaaatgaatctcctaaccaccacaaaaaaagaagaaatgggatcaaaacgccacccatcccataaaaaaaga agatacctcg tcatacttatatgttgtaatatagctcttgttcttatgctcctgtagtgaaaaatttttcttatttcctgcgta Query HRQGHPENUYIPGSEGLQHPRNKLUQKRCINQLLUKLPKDSELAPRSUCUHCRHLIFEKTGSAITUQCTENLPSLCSUQGLRAEENITESCQURLPPAAU |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Target HRQGHPENUYIPGSEGLQHPRNKLUQKRCINQLLUKLPKDSELAPRSUCUHCRHLIFEKTGSAITUQCTENLPSLCSUQGLRAEENITESCQURLPPAAU cacgccgattacgaggtccccaactcaataactctatcagtgtgcaatttctcccatgaagtgaatctagacctttatcgccgggaaagttctctccggt agagacaagatcggagtaacgaatgaaggtaattgatcaacatccgggggaggatttaacgcctcgagcaatcctgggagtgcaaatcacgaggtccccg tggtcagcataaatataataacgcaagatataacaagcataggttgcacattatgataaggtaccagtaactatatcagatgaggtatattgatgtatca * * * * * * * * * Query KTSQQLIPTEASTNUSUKNMAKKUEUPSN ||||||||||||||||||||||||||||| Target KTSQQLIPTEASTNUSUKNMAKKUEUPSN aaacccacaggaaatataaagaatgtcta acgaattccacgcagggaatcaagagcca gatggtataactctacagtgaagaaatac * * * * Sequence until next stop: TAA * Distance CDS-SECIS: 265 ; UGA-SECIS: 274 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = KV389506.1:subseq_2207742,100551_ Strand = - Positions = 49677 - 49608 Found by: Infernal (score=31.58) Covels (score=32.57) Stem2 length = 11 Apical loop length = 14 Insertion in stem2 = 0, 0 Free Energy = -12.54 ..(((((((.......(((((((((((((((...............)))))))))))))))........))))))).. model uucggucaugcgUuaAUGAcGgccuggcccuAAAcCcuuuuuggggcgggccaggcCuGAUGuuuuuucaugaccggc ..({(((((---....{[[{((((((((([(.....-.........)])))))))))}]]}----....)))))}).. target CUACUUGCU---UUAAUGAGAACAGAAACAUAAACU-AUGACCUAGGGGUUUCUGUUGGAU----AAUUAGCAAUUUA **** ^^^ **** _________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:2 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 1e-92 Query name: gi|1044366702|ref|XP_017388639.1| PREDICTED: selenoprotein P isoform X2 [Cebus capucinus imitator] Query range: 1-381 (total 381) Target: /data/www-rg/seblastian_results/32125515/tempfiles/temptarget.fasta Chromosome: KV389506.1:subseq_2207742,100551_ Strand: - Positions: -Exon 1 56008 56210 -Exon 2 54732 54944 -Exon 3 52489 52606 -Exon 4 49943 50551 Redox box: YES Query MWRSLGLALALCLLPSGGTESQDQSSLCKQPPAWSIRDQDPMLNSTGSVTVVALLQASUYLCILQASR <---Intron---> LEDLRVKLEKEGYSNI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| < 1063bp > |||||||||||||||| Target MWRSLGLALALCLLPSGGTESQDQSSLCKQPPAWSIRDQDPMLNSTGSVTVVALLQASUYLCILQASR LEDLRVKLEKEGYSNI ataacgcgcgctccctggagacgcatttacccgtaaagcgcacatagtgagggcccgattctaccgta tggccgacgaggttaa tgggtgtctctgttccggcagaaagctgaacccggtgaaacttaccgctcttcttacggatgttaccg taatgtataaagacat ggacggtcgtctcccaaaagcgcaccatgacacgcaatataggccttagtgttttactacgctggat agacgaaaggaaatttt * Query SYIIVNHQGLSSQLRYTHLKNQVSEHIPVYQQEENQPDVWALLNGNKDDFLIYDR <---Intron---> CGRLVYHLGLPFSFLTFPYVEEAIKIAYC ||||||||||||||||||||||||||||||||||||||||||||||||||||||| < 2125bp > ||||||||||||||||||||||||||||| Target SYIIVNHQGLSSQLRYTHLKNQVSEHIPVYQQEENQPDVWALLNGNKDDFLIYDR CGRLVYHLGLPFSFLTFPYVEEAIKIAYC ttaagaccgcttctataccaacgtgcacgtccggaccggtgctagaaggtcatga tgccgtccgtctttcatctggggaaagtt catttaaagtccatgacataaatcaatctaaaaaaacatgcttagaaaatttaag gggttaatgtctcttctcataactatcag tttttttaacttaaacattgtgtagtttttgaaacaatcgttatacatcccatc atcttattttgttccaacataaactgttct Query EKKCGNCSLT <---Intron---> TLKDEDFCKNVSLATVNKTTEAPQPHHHHKHHRNHGHQHLGSSELSKNQQPGAPDAPTHLTPSDLHHHHKHKGQ |||||||||| < 1937bp > |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Target EKKCGNCSLT TLKDEDFCKNVSLATVNKTTEAPQPHHHHKHHRNHGHQHLGSSELSKNQQPGAPDAPTHLTPSDLHHHHKHKGQ gaatgattca acagggttaagttgagaaaaggcccccccacccacgccccgaagctaacccggcggcaccactgcccccacagc aaaggagctc ctaaaatgaatctcctaaccaccacaaaaaaagaagaaatgggatcaaaacgccacccatcccataaaaaaaga agatacctcg tcatacttatatgttgtaatatagctcttgttcttatgctcctgtagtgaaaaatttttcttatttcctgcgta Query HRQGHPENUYIPGSEGLQHPRNKLUQKRCINQLLUKLPKDSELAPRSUCUHCRHLIFEKTGSAITUQCTENLPSLCSUQGLRAEENITESCQURLPPAAU |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Target HRQGHPENUYIPGSEGLQHPRNKLUQKRCINQLLUKLPKDSELAPRSUCUHCRHLIFEKTGSAITUQCTENLPSLCSUQGLRAEENITESCQURLPPAAU cacgccgattacgaggtccccaactcaataactctatcagtgtgcaatttctcccatgaagtgaatctagacctttatcgccgggaaagttctctccggt agagacaagatcggagtaacgaatgaaggtaattgatcaacatccgggggaggatttaacgcctcgagcaatcctgggagtgcaaatcacgaggtccccg tggtcagcataaatataataacgcaagatataacaagcataggttgcacattatgataaggtaccagtaactatatcagatgaggtatattgatgtatca * * * * * * * * * Query KTSQQLIPTEASTNUSUKNMAKKUEUPSN ||||||||||||||||||||||||||||| Target KTSQQLIPTEASTNUSUKNMAKKUEUPSN aaacccacaggaaatataaagaatgtcta acgaattccacgcagggaatcaagagcca gatggtataactctacagtgaagaaatac * * * * Sequence until next stop: TAA * Distance CDS-SECIS: 688 ; UGA-SECIS: 697 ------- SECIS element -- id:2 -- grade:A ---- Chromosome = KV389506.1:subseq_2207742,100551_ Strand = - Positions = 49254 - 49189 Found by: Infernal (score=30.45) Covels (score=21.41) Stem2 length = 10 Apical loop length = 10 Insertion in stem2 = 1, 0 Free Energy = -12.4 ..(((((((.......((((((((((.(((((...............)))))))))))))))........))))))).. model uucggucaugcgUuaAUGAcGgccug-gcccuAAAcCcuuuuuggggcgggccaggcCuGAUGuuuuuucaugaccggc ...((((((---....{[[{((((((.((((......--.-----...))))))))))}]]}..--..-.))))))... target UUUCCAUAG---UCAAUGAUGGUUUAAUAGGUAAACU--A-----AACCCUAUAAACCUGACCU--CC-UUUAUGGUUA **** ^^^ ****