_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 5e-05 Query name: gi|5107031|gb|AAD39929.1|AF133519_1 thioredoxin reductase 3, partial [Homo sapiens] Query range: 2-577 (total 577) Target: /data/www-rg/seblastian_results/391718330/tempfiles/temptarget.fasta Chromosome: KV389994.1:subseq_492455,100245_ Strand: + Positions: -Exon 1 29164 29205 -Exon 2 34711 34771 -Exon 3 35784 35893 -Exon 4 38706 38810 -Exon 5 39056 39128 -Exon 6 41020 41139 -Exon 7 45044 45186 -Exon 8 49107 49222 -Exon 9 50014 50239 -Exon 10 51500 51592 -Exon 11 58463 58539 -Exon 12 59110 59266 -Exon 13 65249 65356 -Exon 14 66061 66156 -Exon 15 68680 68814 -Exon 16 69335 69400 Redox box: no Query VVIFSKSYCPHSTR <---Intron---> VKELFSSLGVECNVLELDQVD <---Intron---> DGARVQEVLSEITNQKTVPNIFVNKVHVGGCDQ |||||||||||||| < 5505bp > ||||||||| || |||||||| < 1012bp > |||//|||| ||||||||||||||||||||||| Target VVIFSKSYCPHSTR VKELFSSLGAECYVLELDQVD DGAKIQEVLLEITNQKTVPNIFVNKVHVGGCDQ ggataaattccaac gagcttttgggttgtgcgcgg gggaacggctgaaacaagcaatgaagcgggtgc ttttgagagcagcg taattcctgcagattataat aagcataatttatcaaactcatttaatatgggaa ggcccgcctcccgg gaacttcgatattcgattat ttgcgtaaggaacttgacgctttgtagtatatca Query TFQ <---Intron---> AYQSGLLQKLLQEDLAYDYDLIIIGGGSGGLSCAK <---Intron---> EAAILGKKVMVLDFVVPSPQGTSWG <--- ||| < 2812bp > ||||||||||| || |||||||/|||||||||||| < 245bp > ||||||||||||||||||||||||| < Target TFQ AYQSGLLQKLLHEDSAYDYDLIVIGGGSGGLSCAK EAAILGKKVMVLDFVVPSPQGTSWG atc gtcagttcacccggtgtgtgcagagggtggcttga gggatgaagagcgtggctccgattg cta caaggttaattaaaccaaaattttgggcggtcgca accttgaattttatttcccagccg tcg atgttggggcttgtaatttcgccctttttctgtag atctgagatggacttcgatgcatg Query Intron---> LGGTCVNVGCIPKKLMHQAALLGQALCDSRKFGWEYNQQV <---Intron---> RHNWETMTKAIQNHISSLNWGYRLSLREKAVAY 1891bp > ||||||||||/||||||||||||||| ||||||||||||| < 3904bp > |||||||||||||| |||/|| |||||||||| Target LGGTCVNVGCVPKKLMHQAALLGQALHDSRKFGWEYNQQV THNWETMTKAIQNHIRSLNFGYGLSLREKAVAY cggatgaggtgcaataccggctgcgtcgtaatgtgtaccg acatgaaaagacacactcatgtgtttagagggt gtggcgtatggtcaattaaccttgactaacgatggaaaaa tcaagactcactaaatgctatgagtctgaactca tttcttatattttgaggtgtctgggaatcaggtcggttaa ggccggggaagtgcccctactccggtggagtgct Query VNSYGEFVEHHKIK <---Intron---> ATNKKGQETYYTAAQFVIATGERPRYLGIQGDKEYCITS <---Intron---> DDLFSLPYCPGKPLV |||||||||||||| < 3920bp > ||||||||||||||||||||||||||||||||||||||| < 791bp > |||||||||||| || Target VNSYGEFVEHHKIK ATNKKGQETYYTAAQFVIATGERPRYLGIQGDKEYCITS DDLFSLPYCPGKTLV gattggtggccaaa gaaaagcgattaggctgagaggaccttgacggagttaaa ggcttccttcgaatg tacagattaaaata ccaaagaacaacccatttccgagcgatgtagaaaagtcg aattctcagcgactt ctctaattatcaag actaaagatttttagtcaagtagggtaacaataacttc ttctttgttctcagag Query VGASYVALECAGFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYMEQHGVKFLRKFIPVM <---Intron---> VQQLEKGSPGKLKVLAKSTEGTET ||||||||||||||||||||||||||||||||||||||||||||||||||/|| |||||| < 1260bp > |||||||||||||||||||||||| Target VGASYVALECAGFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYMEQHGVRFLWKFIPVM VQQLEKGSPGKLKVLAKSTEGTET gggttggcgtggtcggtgcggagagctacccgtgcgaggaggttagccggatctatacga gcctgagtcgacagtgataggaga tgccatctagcgttcgtgtatctttgctttggtaaatcaatgcataaagtgttgattctt taataagccgatattcaccagcac gtctcccggtagtgtctcatcatgacactctctcaagagagtctgggttggcagacatgg tagggatataggaagtactaaaaa Query IEGVYNT <---Intron---> VLLAIGRDSCTRKIGLEKIGVKINEK <---Intron---> SGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVAI || |||| < 6870bp > |||||||||||||||||||||||||| < 570bp > ||||||||||||/|||||||||||| ||||||||| Target IEEVYNT VLLAIGRDSCTRKIGLEKIGVKINEK SGKIPVNDVEQTSVPYVYAVGDILEGKPELTPVAI agggtaa gttgagcgttaaaagtgaaggaaaga agaacgagggcaagctgtggggatggacgcacgga taataac tttctggacgcgatgtaatgtataaa ggatctaataacgtcatactgattagacatcctct taactca tgattttcctagaaggggttcattg ataaatattgagcttatctttgttggtgagcttcca Query QSGKLLAQRLFGASLEK <---Intron---> CDYINVPTTVFTPLEYGCCGLSEEKAIEVYKKENLE <---Intron---> IYHTLFWPLEWTVAG |||||||/||||||||| < 5982bp > |||||||||||||||||||||||||||||||||||| < 704bp > ||||||||||||||| Target QSGKLLARRLFGASLEK CDYINVPTTVFTPLEYGCCGLSEEKAIEVYKKENLE IYHTLFWPLEWTVAG ctgaccgcactggttga tgtaagcaagtaccgtgttgttggagaggtaagacg atcatttccgtaggg acgattcggttgcctaa gaatatcccttcctaaggggtcaaactataaaaata taacttgctagctcg gacggatgattgctaag ttttttatggtttggttctaataggttaataagtaa atttgcgttagaatc Query RENNTCYAKIICNKFDH <---Intron---> DRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGE <---Intron---> VFTTLE ||||||||||||||||| < 2523bp > ||||||||||||||||||||||||||||||||||||||||||||| < 520bp > |||||| Target RENNTCYAKIICNKFDH DRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGE VFTTLE agaaattgaaataatgc gcgagtcacgcaggggacgtgggaatgcaacccggaagaccatgg gtaatg gaaacgacattgaataa agttgtattgcacgatcagtccctaggtcaattaactgtaccgga ttccta agccttcagacctacct tggagttttaatctgtcactatagactcaagattcctatccatgg gcatga Query ITKSSGLDITQKGCUG |||||||||||||||| Target ITKSSGLDITQKGCUG aaattgcgaacagttg tcaccgtatcaagggg caacaaacctgaccac * Sequence until next stop: TAG * Distance CDS-SECIS: 212 ; UGA-SECIS: 215 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = KV389994.1:subseq_492455,100245_ Strand = + Positions = 69613 - 69687 Found by: Infernal (score=23.92) Covels (score=22.5) Stem2 length = 11 Apical loop length = 14 Insertion in stem2 = 0, 0 Free Energy = -19.44 ..(((((((.......(((((((((((((((................))))))))))))))).........))))))).. model uucggucaugcgUuaAUGAcGgccuggcccuAAAcC-cuuuuuggggcgggccaggcCuGAUGuuu-uuucaugaccggc .....((((---(...{[[{((((({((((((..............))))))})))))}]]}.....-.-)))))..... target GGGACUGCU---UCCUUGAUGCCUUAGCUCGCAGCCCCGUUAUGAGGUGAGCCAAGGCUGAUUCUCG-C-AAGCGAGGAC **** ^^ ****