Command line: [exonerate -m p2g --showtargetgff -q ./SPP00000345_2.0.fa -t ./SPP00000345_2.0/fastasubseq/KV390105.1_fastasubseq.fa] Hostname: [ws101072u] C4 Alignment: ------------ Query: SPP00000345_2.0 # Protein # Selenocysteine synthase (SecS) # Marmoset Target: KV390105.1:subseq(89394,100281) Cebus capucinus imitator isolate Cc_AM_T3 unplaced genomic scaffold Scaffold679, whole genome shotgun sequence:[revcomp] Model: protein2genome:local Raw score: 2443 Query range: 0 -> 493 Target range: 86588 -> 49999 1 : MetAsnArgGluSerPheAlaAlaGlyGluArgLeuValSerProAlaTyrValArgGln : 20 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MetAsnArgGluSerPheAlaAlaGlyGluArgLeuValSerProAlaTyrValArgGln 86588 : ATGAACCGCGAGAGCTTCGCGGCGGGCGAGCGACTGGTGTCGCCGGCTTACGTGAGGCAG : 86531 21 : GlyCysGluAlaArgArgSerHisGluHisLeuIleArgLeuLeuLeuGluLys >>>> : 39 |||||||||||||||||||||||||||||||||||||||||||||||||||||| GlyCysGluAlaArgArgSerHisGluHisLeuIleArgLeuLeuLeuGluLys++ 86530 : GGCTGTGAGGCCCGCCGCTCGCATGAGCACCTCATACGGCTGCTTCTGGAGAAGgt.... : 86472 40 : Target Intron 1 >>>> GlyLysCysProGluAsnGlyTrpAspGluSerThrL : 51 1140 bp ||||||||||||||||||||||||||||||||||||| ++GlyLysCysProGluAsnGlyTrpAspGluSerThrL 86471 : .....................aggGCAAGTGTCCAGAGAATGGCTGGGATGAAAGTACAC : 85298 52 : euGluLeuPheLeuHisGluLeuAlaIleMetAspSerAsnAsnPheLeuGlyAsnCysG : 71 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| euGluLeuPheLeuHisGluLeuAlaIleMetAspSerAsnAsnPheLeuGlyAsnCysG 85297 : TTGAACTCTTTTTACATGAACTTGCAATCATGGACAGCAACAATTTCTTAGGCAATTGTG : 85238 72 : lyValGlyGluArgGluGlyArgValAlaSerAlaLeuValAlaArgArgHisTyr{Ar} : 90 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||{||} lyValGlyGluArgGluGlyArgValAlaSerAlaLeuValAlaArgArgHisTyr{Ar} 85237 : GtgtgggagaaagggaagggagagttGCATCCGCATTAGTTGCTCGTCGTCATTAC{AG} : 85181 91 : >>>> Target Intron 2 >>>> {g}LeuIleHisGlyIleGlyArgSerGlyA : 100 1658 bp {|}!!!||||||||||||||||||||||||| ++ ++{g}PheIleHisGlyIleGlyArgSerGlyA 85180 : gt.........................ag{G}TTCATTCATGGGATTGGACGATCGGGTG : 83493 101 : spIleSerAlaValGlnProLysAlaAlaGlySerSerLeuLeuAsnLysIleThrAsnP : 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||! spIleSerAlaValGlnProLysAlaAlaGlySerSerLeuLeuAsnLysIleThrAsnS 83492 : ATATTTCTGCTGTGCAACCAAAAGCTGCAGGCTCTAGCCTTTTGAACAAAATTACCAATT : 83433 121 : heLeuValLeuAspMetIleLysLeuAla{G} >>>> Target Intron 3 >>>> : 130 !||||||||||||!!:||||||||||||{|} 312 bp erLeuValLeuAspIleIleLysLeuAla{G}++ + 83432 : CTTTGGTCCTGGACATTATCAAGCTGGCT{G}gt.........................a : 83092 131 : {ly}ValHisThrValAlaAsnCysPheValValProMetAlaThrGlyMetSerLeuT : 149 {||}||||||||||||||||||||||||||||||||||||||||||||||||||||||| +{ly}ValHisThrValAlaAsnCysPheValValProMetAlaThrGlyMetSerLeuT 83091 : g{GT}GTCCATACAGTAGCCAACTGCTTTGTAGTTCCTATGGCAACTGGTATGAGTCTAA : 83034 150 : hrLeuCysPheLeuThrLeuArgHisLysArgProLysAlaLysTyrIleIleTrpProA : 169 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| hrLeuCysPheLeuThrLeuArgHisLysArgProLysAlaLysTyrIleIleTrpProA 83033 : CCCTATGTTTCTTAACATTACGACACAAAAGACCAAAGGCAAAGTATATTATATGGCCAC : 82974 170 : rgIleAspGlnLysSerCysPheLysSerMetIleThrAla{G} >>>> Target In : 183 |||||||||||||||||||||||||||||||||||||||||{|} 910 rgIleAspGlnLysSerCysPheLysSerMetIleThrAla{G}++ 82973 : GAATAGACCAGAAGTCCTGCTTTAAATCCATGATCACTGCA{G}gt.............. : 82929 184 : tron 4 >>>> {ly}PheGluProValValIleGluAsnValLeuGluGlyAspGluL : 198 bp {||}||||||||||||||||||||||||||||||||||||||||||| ++{ly}PheGluProValValIleGluAsnValLeuGluGlyAspGluL 82928 : ...........ag{GT}TTTGAGCCTGTGGTgatagaaaatgttttggaagGTGACGAGC : 81977 199 : euArgThrAspLeuGlnAlaValLysAlaLysValGlnGluLeuGlyProAspAsnIleL : 218 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| euArgThrAspLeuGlnAlaValLysAlaLysValGlnGluLeuGlyProAspAsnIleL 81976 : TGCGTACAGACCTGCAAGCAGTAAAGGCTAAAGTCCAGGAACTTGGGCCTGATAACATTC : 81917 219 : euCysIleHisSerThrThrSerCysPheAlaProArgValProAsp{Ar} >>>> Ta : 234 |||||||||||||||||||||||||||||||||||||||||||||||{||} euCysIleHisSerThrThrSerCysPheAlaProArgValProAsp{Ar}++ 81916 : TGTGTATTCATTCTACTACATCCTGTTTTGCTCCGAGGGTGCCTGAT{AG}gt....... : 81865 235 : rget Intron 5 >>>> {g}LeuGluGluLeuAlaValIleCysAlaAsnPheAspI : 247 2899 bp {|}|||||||||||||||||||||||||||||||||! !| ++{g}LeuGluGluLeuAlaValIleCysAlaAsnPheGlyI 81864 : ..................ag{a}ctAGAAGAACTGGCTGTGATTTGTGCTAATTTTGGCA : 78931 248 : leProHisIleValAsnAsnAlaTyrGlyValGlnSerSerLysCysMetHisLeuIleG : 267 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| leProHisIleValAsnAsnAlaTyrGlyValGlnSerSerLysCysMetHisLeuIleG 78930 : TTCCACATATAGTCAATAATGCTTATGGAGTGCAGTCTTCAAAGTGTATGCACCTCATTC : 78871 268 : lnGln >>>> Target Intron 6 >>>> GlyAlaArgValGlyArgIleAspAl : 277 ||||| 6515 bp |||||||||||||||||||||||||| lnGln++ ++GlyAlaArgValGlyArgIleAspAl 78870 : AGCAGgt.........................agGGGGCTCGAGTTGGTAGAATAGATGC : 72326 278 : aPheValGlnSerLeuAspLysAsnPheMetValProValGlyGlyAlaIleIleAlaGl : 297 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| aPheValGlnSerLeuAspLysAsnPheMetValProValGlyGlyAlaIleIleAlaGl 72325 : TTTTGTTCAGAGCTTGGACAAAAATTTTATGGTTCCAGTAGGTGGCGCTATAATTGCTGG : 72266 298 : yPheAsnAspSerPheIleGlnGluIleSerLysMetTyrPro{G} >>>> Target : 312 |||||||||||||||||||||||||||||||||||||||||||{|} 13 yPheAsnAspSerPheIleGlnGluIleSerLysMetTyrPro{G}++ 72265 : CTTTAATGATTCCTTCATTCAGGAAATCAGCAAGATGTATCCA{G}gt............ : 72218 313 : Intron 7 >>>> {ly}ArgAlaSerAlaSerProSerLeuAspValLeuIleThrLe : 326 3 bp {||}||||||||||||||||||||||||||||||||||||||||| ++{ly}ArgAlaSerAlaSerProSerLeuAspValLeuIleThrLe 72217 : .............ag{GA}AGAGCTTCAGCTTCACCTTCTTTAGATGTCCTTATTACTTT : 72046 327 : uLeuSerLeuGlySerAsnGlyTyrLysLysLeuLeuLysGluArgLys >>>> Targ : 343 ||||||||||||||||||||||||||||||||||||||||||||||||| uLeuSerLeuGlySerAsnGlyTyrLysLysLeuLeuLysGluArgLys++ 72045 : ATTATCACTTGGATCAAATGGCTATaagaaactattaaaagaaagaaaggt......... : 71993 344 : et Intron 8 >>>> GluMetPheSerTyrLeuSerAsnGlnIleLysLysLeuSer : 356 18596 bp |||||||||||||||||||||||||||||||||||||||||| ++GluMetPheSerTyrLeuSerAsnGlnIleLysLysLeuSer 71992 : ................aggaaatgttttcatatttgtcCAACCAAATAAAGAAGTTGTCA : 53360 357 : GluAlaTyrAsnGluArgLeuLeuHisThrProHisAsnProIleSerLeu{A} >>>> : 374 |||||||||||||||||||||||||||||||||||||||||||||||||||{|} GluAlaTyrAsnGluArgLeuLeuHisThrProHisAsnProIleSerLeu{A}++ 53359 : GAAGCCTACAATGAAAGACTGTTGCATACACCTCACAATCCCATATCTTTA{G}gt.... : 53303 375 : Target Intron 9 >>>> {la}ValThrLeuLysThrLeuAspLysHisHisAsp : 385 1471 bp {||}||||||||||||||||||||||||||||||||| ++{la}ValThrLeuLysThrLeuAspLysHisHisAsp 53302 : .....................ag{ct}gtgACACTTAAAACATTAGATAAACACCATGAC : 51802 386 : LysAlaValThrGlnLeuGlySerMetLeuPheThrArgGlnValSerGlyAla{Ar} : 404 ||||||||||||||||||||||||||||||||||||||||||||||||||||||{||} LysAlaValThrGlnLeuGlySerMetLeuPheThrArgGlnValSerGlyAla{Ar}++ 51801 : AAAGCTGTCACTCAGCTTGGCTCTATGCTTTTTACCAGACAGGTTTCTGGAGCC{AG}gt : 51743 405 : >>>> Target Intron 10 >>>> {g}ValValProLeuGlySerValGlnThrVa : 414 1476 bp {|}||||||||||||||||||||||||||||| ++{g}ValValProLeuGlySerValGlnThrVa 51742 : ..........................ag{G}GTTGTGCCTCTTGGGTCTGTGCAAACTGT : 50239 415 : lSerGlyTyrThrPheArgGlyPheMetSerHisThrAsnAsnTyrProCysAlaTyrLe : 434 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| lSerGlyTyrThrPheArgGlyPheMetSerHisThrAsnAsnTyrProCysAlaTyrLe 50238 : GAGTGGCTATACTTTCAGAGGCTTTATGTCACATACAAATAATTACCCTTGTGCTTACCT : 50179 435 : uAsnAlaAlaSerAlaIleGlyMetLysMetGlnAspValAspLeuPheIleLysArgLe : 454 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| uAsnAlaAlaSerAlaIleGlyMetLysMetGlnAspValAspLeuPheIleLysArgLe 50178 : CAATGCTGCATCAGCCATCGGAATGAAGATGCAGGATGTGGACCTGTTCATAAAGAGACT : 50119 455 : uAspArgCysLeuLysThrValArgLysGluGlnSerLysAspSerAspAspAsnTyrAs : 474 ||||||||||||||||||||||||||||||||||||||||:::||||||||||||||||| uAspArgCysLeuLysThrValArgLysGluGlnSerLysGluSerAspAspAsnTyrAs 50118 : TGACAGGTGTTTAAAGACagtaagaaaagaacaaagtaaagaGAGTGATGACAATTATGA : 50059 475 : pLysThrGluAspValAspIleGluGluMetAlaLeuLysLeuAspAsnValLeuLeu : 493 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| pLysThrGluAspValAspIleGluGluMetAlaLeuLysLeuAspAsnValLeuLeu 50058 : CAAAACTGAAGATGTGGATATTGAAGAAATGGCTTTAAAACTAGATAATGTACTTCTT : 50000 vulgar: SPP00000345_2.0 0 493 . KV390105.1:subseq(89394,100281) 86588 49999 - 2443 M 38 114 5 0 2 I 0 1136 3 0 2 M 51 153 S 0 2 5 0 2 I 0 1654 3 0 2 S 1 1 M 39 117 S 0 1 5 0 2 I 0 308 3 0 2 S 1 2 M 52 156 S 0 1 5 0 2 I 0 906 3 0 2 S 1 2 M 50 150 S 0 2 5 0 2 I 0 2895 3 0 2 S 1 1 M 34 102 5 0 2 I 0 6511 3 0 2 M 43 129 S 0 1 5 0 2 I 0 129 3 0 2 S 1 2 M 30 90 5 0 2 I 0 18592 3 0 2 M 31 93 S 0 1 5 0 2 I 0 1467 3 0 2 S 1 2 M 29 87 S 0 2 5 0 2 I 0 1472 3 0 2 S 1 1 M 89 267 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2016-11-17 ##type DNA # # # seqname source feature start end score strand frame attributes # KV390105.1:subseq(89394,100281) exonerate:protein2genome:local gene 50000 86588 2443 - . gene_id 1 ; sequence SPP00000345_2.0 ; gene_orientation + KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 86475 86588 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 86475 86588 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 86473 86474 . - . intron_id 1 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 85335 86474 . - . intron_id 1 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 85335 85336 . - . intron_id 0 ; splice_site "ag" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 85180 85334 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 85180 85334 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 85178 85179 . - . intron_id 2 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 83522 85179 . - . intron_id 2 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 83522 83523 . - . intron_id 1 ; splice_site "AG" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 83403 83521 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 83403 83521 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 83401 83402 . - . intron_id 3 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 83091 83402 . - . intron_id 3 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 83091 83092 . - . intron_id 2 ; splice_site "AG" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 82932 83090 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 82932 83090 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 82930 82931 . - . intron_id 4 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 82022 82931 . - . intron_id 4 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 82022 82023 . - . intron_id 3 ; splice_site "AG" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 81868 82021 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 81868 82021 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 81866 81867 . - . intron_id 5 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 78969 81867 . - . intron_id 5 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 78969 78970 . - . intron_id 4 ; splice_site "ag" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 78866 78968 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 78866 78968 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 78864 78865 . - . intron_id 6 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 72351 78865 . - . intron_id 6 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 72351 72352 . - . intron_id 5 ; splice_site "AG" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 72221 72350 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 72221 72350 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 72219 72220 . - . intron_id 7 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 72088 72220 . - . intron_id 7 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 72088 72089 . - . intron_id 6 ; splice_site "AG" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 71996 72087 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 71996 72087 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 71994 71995 . - . intron_id 8 ; splice_site "gt" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 53400 71995 . - . intron_id 8 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 53400 53401 . - . intron_id 7 ; splice_site "ag" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 53306 53399 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 53306 53399 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 53304 53305 . - . intron_id 9 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 51835 53305 . - . intron_id 9 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 51835 51836 . - . intron_id 8 ; splice_site "ag" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 51744 51834 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 51744 51834 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice5 51742 51743 . - . intron_id 10 ; splice_site "GT" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local intron 50268 51743 . - . intron_id 10 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local splice3 50268 50269 . - . intron_id 9 ; splice_site "AG" KV390105.1:subseq(89394,100281) exonerate:protein2genome:local cds 50000 50267 . - . KV390105.1:subseq(89394,100281) exonerate:protein2genome:local exon 50000 50267 . - . insertions 0 ; deletions 0 KV390105.1:subseq(89394,100281) exonerate:protein2genome:local similarity 50000 86588 2443 - . alignment_id 1 ; Query SPP00000345_2.0 ; Align 86589 1 114 ; Align 85335 39 153 ; Align 83521 91 117 ; Align 83089 131 156 ; Align 82020 184 150 ; Align 78968 235 102 ; Align 72351 269 129 ; Align 72086 313 90 ; Align 53400 343 93 ; Align 51833 375 87 ; Align 50267 405 267 # --- END OF GFF DUMP --- # -- completed exonerate analysis