Command line: [exonerate -m p2g --showtargetgff -q ./SPP00000309_2.0.fa -t ./SPP00000309_2.0/fastasubseq/KV389748.1_fastasubseq.fa] Hostname: [ws101072u] C4 Alignment: ------------ Query: SPP00000309_2.0 # Protein # Iodothyronine deiodinase 1 (DIO1) # Marmoset Target: KV389748.1:subseq(962971,100341) Cebus capucinus imitator isolate Cc_AM_T3 unplaced genomic scaffold Scaffold322, whole genome shotgun sequence Model: protein2genome:local Raw score: 1302 Query range: 0 -> 249 Target range: 49999 -> 68429 1 : MetGlyLeuProGlyProGlyLeuTrpLeuLysArgLeuTrpValLeuLeuGluValAla : 20 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MetGlyLeuProGlyProGlyLeuTrpLeuLysArgLeuTrpValLeuLeuGluValAla 50000 : ATGGGGCTGCCCGGGCCAGGGCTGTGGCTGAAGAGGCTCTGGGTGCTCTTGGAGGTGGCT : 50057 21 : ValHisValAlaValGlyLysValLeuLeuThrLeuPheProAspArgValLysLysAsn : 40 |||||||||||||||||||||:!!|||||||||||||||||||||||||||||||||||| ValHisValAlaValGlyLysLeuLeuLeuThrLeuPheProAspArgValLysLysAsn 50058 : GTGCATGTGGCTGTGGGCAAACTGCTTCTGACATTGTTTCCAGACAGAGTCAAGAAGAAC : 50117 41 : IleLeuAlaMetGlyAspLysThrGlyMetThrArgAsnProAsnPheSerHisAspAsn : 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| IleLeuAlaMetGlyAspLysThrGlyMetThrArgAsnProAsnPheSerHisAspAsn 50118 : ATACTAGCCATGGGCGACAAGACGGGTATGACCAGGAACCCAAACTTCAGCCACGACAAC : 50177 61 : TrpIleProThrPhePheSerThrGlnTyrPheTrpPheValLeuLysValArgTrpGln : 80 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| TrpIleProThrPhePheSerThrGlnTyrPheTrpPheValLeuLysValArgTrpGln 50178 : TGGATCCCGACCTTTTTCAGCACCCAGTATTTCTGGTTCGTCCTGAAGGTCCGTTGGCAG : 50237 81 : ArgLeuGluAspThrThrGluLeuGlyGlyLeuAlaProAsnCysProValValHisLeu : 100 |||||||||||||||.!!|||||||||||||||||||||||||||||||||||||||||| ArgLeuGluAspThrAlaGluLeuGlyGlyLeuAlaProAsnCysProValValHisLeu 50238 : CGACTAGAGGACACGGCTGAGCTAGGGGGTCTGGCCCCAAACTGCCCAGTGGTCCACCTC : 50297 101 : SerGlyGlnArgCysAsnIleTrpAspPheMetGln{G} >>>> Target Intron : 113 ||||||||||||||||||||||||||||||||||||{|} 10101 bp SerGlyGlnArgCysAsnIleTrpAspPheMetGln{G}++ 50298 : TCAGGACAGAGGTGCAACATTTGGGACTTTATGCAA{G}gt................... : 50339 114 : 1 >>>> {ly}AsnArgProLeuValLeuAsnPheGlySerCysThrUnkProSerPhe : 129 {||}|||||||||||||||||||||||||||||||||||| ||||||||| ++{ly}AsnArgProLeuValLeuAsnPheGlySerCysThr***ProSerPhe 50340 : ......ag{GA}AACAGGCCCCTGGTGCTGAATTTTGGAAGTTGTACCTGACCTTCATTT : 60485 130 : MetPheLysPheAspGlnPheLysArgLeuThrGluAspPheSerSerValAlaAspPhe : 149 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MetPheLysPheAspGlnPheLysArgLeuThrGluAspPheSerSerValAlaAspPhe 60486 : ATGTTCAAATTTGACCAATTCAAGAGACTTACTGAAGACTTTAGTTCTGTAGCAGATTTT : 60545 150 : LeuIleIleTyrIleGluGluAlaHisAlaSer{A} >>>> Target Intron 2 > : 161 |||||||||||||||||||||||||||||||||{|} 4570 bp LeuIleIleTyrIleGluGluAlaHisAlaSer{A}++ 60546 : CTCATCATTTACATTGAAGAAGCACATGCATCA{G}gt...................... : 60584 162 : >>> {sp}GlyTrpAlaPheLysAsnAsnValAspIleArgAsnHisGlnAsnLeuGln : 178 {||}||||||||||||||||||||||||||||||||||||||||||||||||||| ++{sp}GlyTrpAlaPheLysAsnAsnValAspIleArgAsnHisGlnAsnLeuGln 60585 : ...ag{AT}GGCTGGGCTTTTAAGAACAACGTGGACATCAGGAATCACCAGAACCTTCAG : 65202 179 : AspArgLeuGlnAlaAlaHisLeuLeuLeuAlaArgSerProGlnCysProValValVal : 198 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| AspArgLeuGlnAlaAlaHisLeuLeuLeuAlaArgSerProGlnCysProValValVal 65203 : GACCGCCTGCAGGCAGCCCATCTGCTGCTGGCCAGGAGCCCCCAGTGCCCTGTGGTGGTG : 65262 199 : AspThrMetGlnAsnGlnSerSerGluLeuTyrAlaAlaLeuProGluArgLeuTyrIle : 218 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| AspThrMetGlnAsnGlnSerSerGluLeuTyrAlaAlaLeuProGluArgLeuTyrIle 65263 : GACACGATGCAGAACCAGAGCAGTGAGCTCTACGCAGCACTGCCGGAAAGGCTCTACATA : 65322 219 : IleGlnGluGlyArgIleLeuTyrLys >>>> Target Intron 3 >>>> GlyL : 229 ||||||||||||||||||||||||||| 3012 bp |||| IleGlnGluGlyArgIleLeuTyrLys++ ++GlyL 65323 : ATCCAGGAGGGCAGGATCCTCTACAAGgt.........................agGGTA : 68367 230 : ysSerGlyProTrpAsnTyrAsnProGluGluValArgAspValLeuGluLysLeuHisS : 249 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ysSerGlyProTrpAsnTyrAsnProGluGluValArgAspValLeuGluLysLeuHisS 68368 : AATCCGGCCCTTGGAACTACAATCCAGAGGAAGTTCGTGATGTTCTGGAAAAACTCCATA : 68427 250 : er : 249 || er 68428 : GT : 68429 vulgar: SPP00000309_2.0 0 249 . KV389748.1:subseq(962971,100341) 49999 68429 + 1302 M 112 336 S 0 1 5 0 2 I 0 10097 3 0 2 S 1 2 M 47 141 S 0 1 5 0 2 I 0 4566 3 0 2 S 1 2 M 66 198 5 0 2 I 0 3008 3 0 2 M 22 66 # --- START OF GFF DUMP --- # # ##gff-version 2 ##source-version exonerate:protein2genome:local 2.2.0 ##date 2016-11-17 ##type DNA # # # seqname source feature start end score strand frame attributes # KV389748.1:subseq(962971,100341) exonerate:protein2genome:local gene 50000 68429 1302 + . gene_id 1 ; sequence SPP00000309_2.0 ; gene_orientation + KV389748.1:subseq(962971,100341) exonerate:protein2genome:local cds 50000 50336 . + . KV389748.1:subseq(962971,100341) exonerate:protein2genome:local exon 50000 50336 . + . insertions 0 ; deletions 0 KV389748.1:subseq(962971,100341) exonerate:protein2genome:local splice5 50337 50338 . + . intron_id 1 ; splice_site "GT" KV389748.1:subseq(962971,100341) exonerate:protein2genome:local intron 50337 60437 . + . intron_id 1 KV389748.1:subseq(962971,100341) exonerate:protein2genome:local splice3 60436 60437 . + . intron_id 0 ; splice_site "AG" KV389748.1:subseq(962971,100341) exonerate:protein2genome:local cds 60438 60581 . + . KV389748.1:subseq(962971,100341) exonerate:protein2genome:local exon 60438 60581 . + . insertions 0 ; deletions 0 KV389748.1:subseq(962971,100341) exonerate:protein2genome:local splice5 60582 60583 . + . intron_id 2 ; splice_site "GT" KV389748.1:subseq(962971,100341) exonerate:protein2genome:local intron 60582 65151 . + . intron_id 2 KV389748.1:subseq(962971,100341) exonerate:protein2genome:local splice3 65150 65151 . + . intron_id 1 ; splice_site "AG" KV389748.1:subseq(962971,100341) exonerate:protein2genome:local cds 65152 65351 . + . KV389748.1:subseq(962971,100341) exonerate:protein2genome:local exon 65152 65351 . + . insertions 0 ; deletions 0 KV389748.1:subseq(962971,100341) exonerate:protein2genome:local splice5 65352 65353 . + . intron_id 3 ; splice_site "GT" KV389748.1:subseq(962971,100341) exonerate:protein2genome:local intron 65352 68363 . + . intron_id 3 KV389748.1:subseq(962971,100341) exonerate:protein2genome:local splice3 68362 68363 . + . intron_id 2 ; splice_site "AG" KV389748.1:subseq(962971,100341) exonerate:protein2genome:local cds 68364 68429 . + . KV389748.1:subseq(962971,100341) exonerate:protein2genome:local exon 68364 68429 . + . insertions 0 ; deletions 0 KV389748.1:subseq(962971,100341) exonerate:protein2genome:local similarity 50000 68429 1302 + . alignment_id 1 ; Query SPP00000309_2.0 ; Align 50000 1 336 ; Align 60440 114 141 ; Align 65154 162 198 ; Align 68364 228 66 # --- END OF GFF DUMP --- # -- completed exonerate analysis