_________________________________.______________________._________________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_exonerate category:QUE_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_________________________________________________________________________________ Blastx evalue: 8e-21 Query name: gi|30231240|ref|NP_840079.1| 15 kDa selenoprotein precursor [Danio rerio] >gi|172046121|sp|Q802F3.2|SEP15_DANRE RecName: Full=15 kDa selenoprotein; Flags: Precursor >gi|29165356|gb|AAO65272.1| 15 kDa selenoprotein SeP15 [Danio rerio] Query range: 6-153 (total 153) Target: /data/www-rg/seblastian_results/849535871/tempfiles/temptarget.fasta Chromosome: LOCP02000277.1:subseq_1735833,200000_ Strand: + Positions: -Exon 1 35315 35512 -Exon 2 49997 50060 -Exon 3 56647 56696 -Exon 4 57364 57492 Redox box: no Query YLLWLLPLLQGLASYGAELSSEACRELGFSSNLLCSSCELLGQFSLNQLDLPCRQCCQEEAQLENRK <---Intron---> LYPGAILEVCGUKLGRF |/|/|/ | ////||| ||||| |||||||||||||/|||||/| ||| || ||||||| | /| < 14484bp > || |||||||||||||| Target YVLFLIFYFQ-VSAFGAEFSSEACSELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKK LYAGAILEVCGUKLGRF tgttcatttc-gtgtgggtttggtagtgttaatctattgccgctaccccgcgtagttcgggctgaaa ctgggacggtgtatgat attttttata-tcctgcatccacggatgtcgattggcgattgatattatacagggggaaacatacaa tacgcttatgggatggt ttatcttttg-gtacgagtaaatccgacccccgtccctcttaatcgtggttctaactgaaagcacag ataatttactaaagggc * Query PQVQA <---Intron---> FVRSDKPKLFRGLQIK <---Intron---> YVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI ||||| < 6586bp > |||||||||||||||| < 667bp > ||||||||||||||||||||||||||||||||||||||||||| Target PQVQA FVRSDKPKLFRGLQIK YVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI ccgcg tgaagacactagccaa tgcgtgcgcactggagaagggcaacataagagggtcagatgca cata cttggaacattggtata atggcacttattaaagatcaatgttagacagtaattgaatagt taca ttcgttacagcaaaacg ttttgctaagtgtctgcttaaacccagcactaaacataggaca Sequence until next stop: TAA * Distance CDS-SECIS: 583 ; UGA-SECIS: 790 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = LOCP02000277.1:subseq_1735833,200000_ Strand = + Positions = 58076 - 58157 Found by: Infernal (score=29.22) Covels (score=29.65) Stem2 length = 11 Apical loop length = 15 Insertion in stem2 = 0, 1 Free Energy = -17.35 ..(((((((.........(((((((((((((((...............))))))))).))))))..........))))))).. model uucggucaugcg--UuaAUGAcGgccuggcccuAAAcCcuuuuuggggcgggccagg-cCuGAUGuu-u-uuucaugaccggc ((((((((((........{[[((((((((((((...............))))))))).)))]]}......-..)))))))))) target CACAAAGAUUAGCAUUAAUGAAGACUACACAGAAAACCUUUUUGAGGAUUUGUGUGGAUCUGAUAUUUAG-CAAAUUUUUGUG **** ^^^ ****