TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a 25 sequences; 708,396,389 total letters Query= SPP00000656_2.0 # Protein # Selenoprotein U3 (SELENOU3) # Zebrafish Length=179 Score E Sequences producing significant alignments: (Bits) Value KQ557213.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 110 5e-26 KQ557215.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 36.2 0.021 KQ557214.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 33.5 1.3 > KQ557213.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold12, whole genome shotgun sequence Length=28566418 Score = 110 bits (275), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 48/67 (72%), Positives = 61/67 (91%), Gaps = 0/67 (0%) Frame = -1 Query 1 VEIGSLWREQAVVLFFLRRFGCQVCRWMAAEVSKLEKDLKAHGIALVGIGPEETGVKEFK 60 VE+ SLW+ Q VV+FFLRRFGCQVCRWMA+E+SKLE DL+A+G++L G+GPEE G++EFK Sbjct 16879177 VELQSLWQNQPVVVFFLRRFGCQVCRWMASEISKLEPDLRANGVSLAGVGPEELGLQEFK 16878998 Query 61 DGGFFKG 67 +GGFFKG Sbjct 16878997 EGGFFKG 16878977 Score = 60.1 bits (144), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 28/39 (72%), Positives = 33/39 (85%), Gaps = 0/39 (0%) Frame = -2 Query 132 GGEKVLLHFIQKSPADNPPLEEITKALGISANVQAGEKP 170 GGEKVLLHFIQ SP D PL++I+ ALGISA V+AG+KP Sbjct 16876464 GGEKVLLHFIQDSPGDFVPLDDISTALGISATVKAGQKP 16876348 Score = 45.4 bits (106), Expect = 1e-04, Method: Composition-based stats. Identities = 22/27 (81%), Positives = 25/27 (93%), Gaps = 0/27 (0%) Frame = -1 Query 106 KASAEGIQGNFSGDLLQSGGMLIVAKG 132 +ASA GIQGNFSGDLLQSGG++IV KG Sbjct 16878004 QASAVGIQGNFSGDLLQSGGLIIVTKG 16877924 Score = 37.7 bits (86), Expect = 0.062, Method: Composition-based stats. Identities = 15/22 (68%), Positives = 21/22 (95%), Gaps = 0/22 (0%) Frame = -3 Query 85 RYNAINVVPAAMGKKVREIASK 106 RY A++V+PAA+GKKVR+I+SK Sbjct 16878227 RYTALSVLPAALGKKVRDISSK 16878162 Score = 37.4 bits (85), Expect = 0.082, Method: Composition-based stats. Identities = 14/17 (82%), Positives = 16/17 (94%), Gaps = 0/17 (0%) Frame = -3 Query 69 IYIDEMKQCYKDLGFKR 85 IY+DE K+CYKDLGFKR Sbjct 16878692 IYVDEEKKCYKDLGFKR 16878642 > KQ557215.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold14, whole genome shotgun sequence Length=28402361 Score = 36.2 bits (82), Expect(2) = 0.021, Method: Compositional matrix adjust. Identities = 19/53 (36%), Positives = 31/53 (58%), Gaps = 4/53 (8%) Frame = +2 Query 29 AAEVSKLEKDLKAHGIALVGIGPEETG--VKEFKDGGFFKGDIYIDEMKQCYK 79 A+E+S L+ L+ G+ LV + E G +++F+ F GDIY+DEM + Sbjct 14670503 ASELSSLKPQLEELGVPLVAVVKENVGSEIQDFR--PHFAGDIYVDEMVSSWN 14670655 Score = 21.2 bits (43), Expect(2) = 0.021, Method: Compositional matrix adjust. Identities = 9/29 (31%), Positives = 18/29 (62%), Gaps = 0/29 (0%) Frame = +3 Query 5 SLWREQAVVLFFLRRFGCQVCRWMAAEVS 33 SLW + V+ +RR G +CR + ++++ Sbjct 14670348 SLWEKNGAVVMAVRRPG*FLCREVVSDLT 14670434 Score = 31.2 bits (69), Expect = 8.9, Method: Compositional matrix adjust. Identities = 10/26 (38%), Positives = 17/26 (65%), Gaps = 0/26 (0%) Frame = +1 Query 18 RRFGCQVCRWMAAEVSKLEKDLKAHG 43 R + C++C + A+ SKL + +K HG Sbjct 6296947 RPYKCELCNYACAQSSKLTRHMKTHG 6297024 > KQ557214.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold13, whole genome shotgun sequence Length=22457735 Score = 33.5 bits (75), Expect = 1.3, Method: Compositional matrix adjust. Identities = 18/47 (38%), Positives = 30/47 (64%), Gaps = 4/47 (9%) Frame = +2 Query 126 MLIVAKGGEKVLLHFIQKSPADNPPLEEITKALGISANVQAGEKPQC 172 +L+V++G E + +H Q +P PP ++ + LGIS +V + E PQC Sbjct 7956845 LLMVSRGLEVISIHN-QDNP---PPPSQVCRHLGISESVGSSEVPQC 7956973 Lambda K H a alpha 0.319 0.138 0.410 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 15348403135 Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a Posted date: Oct 21, 2016 4:48 PM Number of letters in database: 708,396,389 Number of sequences in database: 25 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40