TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a 25 sequences; 708,396,389 total letters Query= SPP00000648_2.0 # Protein # Selenoprotein S (SELENOS) # Zebrafish Length=190 Score E Sequences producing significant alignments: (Bits) Value KQ557219.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 48.9 9e-11 KQ557213.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 31.6 7.4 > KQ557219.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold18, whole genome shotgun sequence Length=33548221 Score = 48.9 bits (115), Expect(2) = 9e-11, Method: Compositional matrix adjust. Identities = 24/35 (69%), Positives = 29/35 (83%), Gaps = 0/35 (0%) Frame = +1 Query 73 DVGSVVRRQEALEASRRRMQEEQDARAAEFREKQR 107 DV V +RQEA+EA+RRRMQ E DA+AA F+EKQR Sbjct 1372252 DVSLVTKRQEAMEAARRRMQAELDAKAAAFKEKQR 1372356 Score = 38.1 bits (87), Expect(2) = 9e-11, Method: Compositional matrix adjust. Identities = 18/23 (78%), Positives = 21/23 (91%), Gaps = 0/23 (0%) Frame = +2 Query 110 EEEKRRQKIEMWDSMQEGKSYKG 132 EEEKRRQKIE W+SM++GKS KG Sbjct 1372433 EEEKRRQKIEEWESMKQGKSSKG 1372501 Score = 44.3 bits (103), Expect = 5e-04, Method: Compositional matrix adjust. Identities = 20/30 (67%), Positives = 22/30 (73%), Gaps = 0/30 (0%) Frame = +2 Query 26 PSVTAFMSEYGWYLLFGCVGVYLLIQHLRK 55 P+V F+S YGWYLL V V LLIQHLRK Sbjct 1371038 PAVGEFLSLYGWYLLAVTVTVLLLIQHLRK 1371127 Score = 37.0 bits (84), Expect = 0.14, Method: Compositional matrix adjust. Identities = 17/26 (65%), Positives = 24/26 (92%), Gaps = 1/26 (4%) Frame = +3 Query 139 QNTEEA-ASSSSLRPKTEKKPLRSSG 163 QNTEEA A+++ ++PKT+KKPLRS+G Sbjct 1372674 QNTEEASAAAAGVKPKTDKKPLRSNG 1372751 > KQ557213.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold12, whole genome shotgun sequence Length=28566418 Score = 31.6 bits (70), Expect = 7.4, Method: Compositional matrix adjust. Identities = 19/43 (44%), Positives = 26/43 (60%), Gaps = 4/43 (9%) Frame = -1 Query 11 NEDVPPQNQDLSFLQPSVTAFMSEYGWYLLFG-CVGVYLLIQH 52 ++D+ P SFL PS T+ M +YLL G C+ +YLL QH Sbjct 13460002 SDDMYPSQ---SFLYPSFTSSMGLINFYLLRGTCILLYLLSQH 13459883 Lambda K H a alpha 0.312 0.126 0.358 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 17709694050 Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a Posted date: Oct 21, 2016 4:48 PM Number of letters in database: 708,396,389 Number of sequences in database: 25 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40