TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a 25 sequences; 708,396,389 total letters Query= SPP00000626_2.0 # Protein # Methionine sulfoxide reductase A (MsrA) # Zebrafish Length=232 Score E Sequences producing significant alignments: (Bits) Value KQ557207.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 83.6 4e-25 KQ557211.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 77.8 1e-14 KQ557225.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 33.5 2.6 > KQ557207.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold6, whole genome shotgun sequence Length=23593460 Score = 83.6 bits (205), Expect(2) = 4e-25, Method: Compositional matrix adjust. Identities = 39/43 (91%), Positives = 39/43 (91%), Gaps = 0/43 (0%) Frame = -2 Query 67 LFGMGCFWGAERKFWRQKGVYSTQVGYSGGYTPNPTYEEVCTG 109 L GMGCFW AERKFW QKGVYSTQVGYSGGYTPN TYEEVCTG Sbjct 2734036 L*GMGCFWEAERKFWTQKGVYSTQVGYSGGYTPNATYEEVCTG 2733908 Score = 52.8 bits (125), Expect(2) = 4e-25, Method: Compositional matrix adjust. Identities = 21/25 (84%), Positives = 24/25 (96%), Gaps = 0/25 (0%) Frame = -1 Query 45 SAKHDVNGNRTVPPFPEGLQMVLFG 69 +AKHDVNGNRT+PPFPEG QMV+FG Sbjct 2734208 AAKHDVNGNRTIPPFPEGTQMVMFG 2734134 Score = 99.8 bits (247), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 46/53 (87%), Positives = 49/53 (92%), Gaps = 0/53 (0%) Frame = -3 Query 179 KVLTEEGFGAITTEITMAKEFYYAEDYHQQYLSKNPDGYCGLGGTGVSCPIGL 231 +VL EEGFG+ITTEIT AK FYYAEDYHQQYLSKNP GYCGLGGTGV+CPIGL Sbjct 2714751 QVLAEEGFGSITTEITEAKPFYYAEDYHQQYLSKNPHGYCGLGGTGVTCPIGL 2714593 Score = 68.6 bits (166), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 30/42 (71%), Positives = 34/42 (81%), Gaps = 0/42 (0%) Frame = -3 Query 109 GKTGHTEVVRVVFEPQKIKFSELLKVFWESHNPTQGMRQGND 150 G+TGHTEVVRVV+ P+KI F+ LLKVFWESHNPTQG D Sbjct 2728185 GRTGHTEVVRVVYHPEKISFNSLLKVFWESHNPTQGKHNIRD 2728060 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 28/37 (76%), Positives = 34/37 (92%), Gaps = 0/37 (0%) Frame = -1 Query 144 GMRQGNDVGTTYRSSIYTNTQEQLEQALQSREEYQKV 180 GMRQGNDVGTTYRS+IY TQ+QLE+AL S+++YQKV Sbjct 2726666 GMRQGNDVGTTYRSAIYAYTQQQLEEALASKDQYQKV 2726556 > KQ557211.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold10, whole genome shotgun sequence Length=26077971 Score = 77.8 bits (190), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 37/60 (62%), Positives = 44/60 (73%), Gaps = 4/60 (7%) Frame = -1 Query 69 GMGCFWGAERKFWRQKGVYSTQVGYSGGYTPNPTYEEVCTGKTGHTEVVRVVFEPQKIKF 128 GMGCFWGAE+ FWR GV+STQVG++GG+TPNPTY EVCTG H +VF P + F Sbjct 4128762 GMGCFWGAEKLFWRLPGVFSTQVGFAGGFTPNPTYNEVCTG-IAH---FLLVFTPPTL*F 4128595 Score = 74.7 bits (182), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 34/50 (68%), Positives = 39/50 (78%), Gaps = 0/50 (0%) Frame = -2 Query 181 LTEEGFGAITTEITMAKEFYYAEDYHQQYLSKNPDGYCGLGGTGVSCPIG 230 L ++G+G ITTEI +EF+YAEDYHQQYL K P GYCGL GTGV CP G Sbjct 4099718 LDKKGYGPITTEILEGQEFFYAEDYHQQYLKKVPKGYCGLKGTGVYCPTG 4099569 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 26/42 (62%), Positives = 31/42 (74%), Gaps = 0/42 (0%) Frame = -3 Query 108 TGKTGHTEVVRVVFEPQKIKFSELLKVFWESHNPTQGMRQGN 149 +G T HTEVVRVVF PQ I ELL+ FWE+H+PTQG R + Sbjct 4125220 SGLTAHTEVVRVVFSPQDISLEELLRQFWENHDPTQGRRSAH 4125095 Score = 48.5 bits (114), Expect = 3e-05, Method: Compositional matrix adjust. Identities = 22/43 (51%), Positives = 31/43 (72%), Gaps = 0/43 (0%) Frame = -1 Query 142 TQGMRQGNDVGTTYRSSIYTNTQEQLEQALQSREEYQKVLTEE 184 + GM+Q ND GT YRS IY ++ Q E AL+S+E +QKV+T + Sbjct 4111716 SAGMKQHNDSGTQYRSVIYPSSPAQQEAALKSKEAFQKVITHK 4111588 Score = 35.8 bits (81), Expect = 0.49, Method: Compositional matrix adjust. Identities = 14/26 (54%), Positives = 20/26 (77%), Gaps = 0/26 (0%) Frame = -2 Query 44 VSAKHDVNGNRTVPPFPEGLQMVLFG 69 ++ KH VNGN TV PFP G++ ++FG Sbjct 4128986 LTDKHAVNGNPTVEPFPGGMETIMFG 4128909 > KQ557225.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold24, whole genome shotgun sequence Length=55650406 Score = 33.5 bits (75), Expect = 2.6, Method: Compositional matrix adjust. Identities = 16/52 (31%), Positives = 25/52 (48%), Gaps = 3/52 (6%) Frame = -2 Query 72 CFWGAERKFWRQKGVYSTQVGYSGGYTPNPTYEEVCTGKTGHTEVVRVVFEP 123 CFW E + +++ Y +G G+ P+PT CT G V+ + F P Sbjct 45550266 CFWCLENEAFQKPSNYDVTIG---GHCPSPTRCMHCTMAAGKGSVLLLTFHP 45550120 Lambda K H a alpha 0.316 0.133 0.404 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 26918726406 Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a Posted date: Oct 21, 2016 4:48 PM Number of letters in database: 708,396,389 Number of sequences in database: 25 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40