TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a 25 sequences; 708,396,389 total letters Query= SPP00000625_2.0 # Protein # Methionine sulfoxide reductase A (MsrA) # Zebrafish Length=238 Score E Sequences producing significant alignments: (Bits) Value KQ557207.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 69.7 4e-15 KQ557211.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 78.6 8e-15 KQ557219.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 32.0 8.3 > KQ557207.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold6, whole genome shotgun sequence Length=23593460 Score = 69.7 bits (169), Expect(2) = 4e-15, Method: Compositional matrix adjust. Identities = 34/57 (60%), Positives = 40/57 (70%), Gaps = 3/57 (5%) Frame = -2 Query 71 GMGCFWGAERRFWKITGVFSTQVGYAGGFTSNPTYHEVCSG---LTGHTEVVRVVFS 124 GMGCFW AER+FW GV+STQVGY+GG+T N TY EVC+G + HT VFS Sbjct 2734030 GMGCFWEAERKFWTQKGVYSTQVGYSGGYTPNATYEEVCTGSLCIPTHTHTHMHVFS 2733860 Score = 33.1 bits (74), Expect(2) = 4e-15, Method: Compositional matrix adjust. Identities = 12/19 (63%), Positives = 16/19 (84%), Gaps = 0/19 (0%) Frame = -1 Query 53 NGNTTVEPFPDGMEMIMFG 71 NGN T+ PFP+G +M+MFG Sbjct 2734190 NGNRTIPPFPEGTQMVMFG 2734134 Score = 74.3 bits (181), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 34/55 (62%), Positives = 40/55 (73%), Gaps = 0/55 (0%) Frame = -3 Query 181 EALSRKGLGEITTDIRQLTVFYYAEDYHQQYLHKVPKGYCGLKGTGVTCSITEGR 235 + L+ +G G ITT+I + FYYAEDYHQQYL K P GYCGL GTGVTC I G+ Sbjct 2714751 QVLAEEGFGSITTEITEAKPFYYAEDYHQQYLSKNPHGYCGLGGTGVTCPIGLGK 2714587 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 27/43 (63%), Positives = 32/43 (74%), Gaps = 0/43 (0%) Frame = -3 Query 110 SGLTGHTEVVRVVFSPKDVSLEKLLKVFWESHDPTQGMAQGND 152 +G TGHTEVVRVV+ P+ +S LLKVFWESH+PTQG D Sbjct 2728188 AGRTGHTEVVRVVYHPEKISFNSLLKVFWESHNPTQGKHNIRD 2728060 Score = 44.3 bits (103), Expect = 0.001, Method: Compositional matrix adjust. Identities = 21/36 (58%), Positives = 26/36 (72%), Gaps = 0/36 (0%) Frame = -1 Query 146 GMAQGNDHGTQYRSAIYTYSPEQQDLAMKSKHMYQE 181 GM QGND GT YRSAIY Y+ +Q + A+ SK YQ+ Sbjct 2726666 GMRQGNDVGTTYRSAIYAYTQQQLEEALASKDQYQK 2726559 > KQ557211.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold10, whole genome shotgun sequence Length=26077971 Score = 78.6 bits (192), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 36/58 (62%), Positives = 46/58 (79%), Gaps = 4/58 (7%) Frame = -1 Query 71 GMGCFWGAERRFWKITGVFSTQVGYAGGFTSNPTYHEVCSGLTGHTEVVRVVFSPKDV 128 GMGCFWGAE+ FW++ GVFSTQVG+AGGFT NPTY+EVC+G+ H +VF+P + Sbjct 4128762 GMGCFWGAEKLFWRLPGVFSTQVGFAGGFTPNPTYNEVCTGIA-H---FLLVFTPPTL 4128601 Score = 76.3 bits (186), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 36/56 (64%), Positives = 42/56 (75%), Gaps = 0/56 (0%) Frame = -2 Query 183 LSRKGLGEITTDIRQLTVFYYAEDYHQQYLHKVPKGYCGLKGTGVTCSITEGRDEL 238 L +KG G ITT+I + F+YAEDYHQQYL KVPKGYCGLKGTGV C ++EL Sbjct 4099718 LDKKGYGPITTEILEGQEFFYAEDYHQQYLKKVPKGYCGLKGTGVYCPTGGSKEEL 4099551 Score = 71.2 bits (173), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 32/50 (64%), Positives = 39/50 (78%), Gaps = 0/50 (0%) Frame = -3 Query 110 SGLTGHTEVVRVVFSPKDVSLEKLLKVFWESHDPTQGMAQGNDHGTQYRS 159 SGLT HTEVVRVVFSP+D+SLE+LL+ FWE+HDPTQG + + Q S Sbjct 4125220 SGLTAHTEVVRVVFSPQDISLEELLRQFWENHDPTQGRRSAHPNKPQSPS 4125071 Score = 51.6 bits (122), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 23/43 (53%), Positives = 30/43 (70%), Gaps = 0/43 (0%) Frame = -1 Query 144 TQGMAQGNDHGTQYRSAIYTYSPEQQDLAMKSKHMYQEALSRK 186 + GM Q ND GTQYRS IY SP QQ+ A+KSK +Q+ ++ K Sbjct 4111716 SAGMKQHNDSGTQYRSVIYPSSPAQQEAALKSKEAFQKVITHK 4111588 Score = 37.0 bits (84), Expect = 0.20, Method: Compositional matrix adjust. Identities = 16/19 (84%), Positives = 16/19 (84%), Gaps = 0/19 (0%) Frame = -2 Query 53 NGNTTVEPFPDGMEMIMFG 71 NGN TVEPFP GME IMFG Sbjct 4128965 NGNPTVEPFPGGMETIMFG 4128909 > KQ557219.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold18, whole genome shotgun sequence Length=33548221 Score = 32.0 bits (71), Expect = 8.3, Method: Compositional matrix adjust. Identities = 16/37 (43%), Positives = 21/37 (57%), Gaps = 1/37 (3%) Frame = -1 Query 90 STQVGYAGGFTSNPTYHEVCSGLTGHTEVVRVVFSPK 126 + + YAG F S P H CSGLT HT ++R P+ Sbjct 28282261 ANSLRYAGTF-SLPRPHSFCSGLTNHTVILRQSILPQ 28282154 Lambda K H a alpha 0.319 0.136 0.414 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 28335501480 Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a Posted date: Oct 21, 2016 4:48 PM Number of letters in database: 708,396,389 Number of sequences in database: 25 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40