TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a 25 sequences; 708,396,389 total letters Query= SPP00000619_2.0 # Protein # Glutathione peroxidase 7 (GPx7) # Zebrafish Length=147 Score E Sequences producing significant alignments: (Bits) Value KQ557224.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 168 3e-46 KQ557204.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 104 3e-24 KQ557216.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 45.4 8e-05 KQ557211.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 44.3 2e-04 KQ557202.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 41.6 0.002 KQ557213.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 37.4 0.036 KQ557217.1 Xiphophorus couchianus unplaced genomic scaffold Sca... 31.6 4.5 > KQ557224.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold23, whole genome shotgun sequence Length=30408201 Score = 168 bits (425), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 76/88 (86%), Positives = 83/88 (94%), Gaps = 0/88 (0%) Frame = +3 Query 1 QVSLAVNVASECGYTDEHYKDLQQLQKDFGPFHFNVLAFPCNQFGQQEPGSDKEIDSFVR 60 QVSL VNVASECG+TD+HY+DLQQLQ+DFGPFHFNVLAFPCNQFGQQEPGSDK+IDSFVR Sbjct 25623405 QVSLVVNVASECGFTDQHYRDLQQLQRDFGPFHFNVLAFPCNQFGQQEPGSDKDIDSFVR 25623584 Query 61 RVYGVSFPIFSKIAVVGIGANNAYKYLG 88 VYGVSFP+FSKIAVVG GANN YK+L Sbjct 25623585 TVYGVSFPLFSKIAVVGTGANNVYKHLA 25623668 Score = 74.3 bits (181), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 31/46 (67%), Positives = 39/46 (85%), Gaps = 0/46 (0%) Frame = +2 Query 102 SEASRKEPTWNFWKYLIDTDGKVVDAWGPEVSVKEIRPRITEMVRK 147 ++ S KEP WNFWK+LID DG+VVDAWGP+V V EIRP+I +MVR+ Sbjct 25625096 ADVSGKEPDWNFWKFLIDVDGRVVDAWGPKVPVTEIRPKIADMVRQ 25625233 Score = 31.2 bits (69), Expect = 5.6, Method: Composition-based stats. Identities = 34/124 (27%), Positives = 58/124 (47%), Gaps = 23/124 (19%) Frame = -2 Query 13 GYTDEHYKDLQQLQKDFGPFHFNVLAFPCNQFGQQEPGSDKEIDSFVRRVYGVSFPIFSK 72 GY + +++QL +F F+F++ C F ++ SDK I SF + F IF + Sbjct 21327944 GYKLTNIVNMRQL*TEFCKFYFHL---ACLTFLKRRHESDK-IPSFPGEI--CIFIIFLR 21327783 Query 73 IAVV---------GIGANNAYKYL--------GLLSSKHSSVFLLISEASRKEPTWNFWK 115 I+ V G +++ +L L+ S + SV + +RK+ N+WK Sbjct 21327782 ISAVCKP*KPAE*GCWESSSLHFLMRARKKLIKLIRSANISVL*FLF*KNRKQCLENYWK 21327603 Query 116 YLID 119 YLI+ Sbjct 21327602 YLIN 21327591 Score = 30.4 bits (67), Expect = 8.9, Method: Compositional matrix adjust. Identities = 11/23 (48%), Positives = 14/23 (61%), Gaps = 0/23 (0%) Frame = +1 Query 33 HFNVLAFPCNQFGQQEPGSDKEI 55 HF LAFPC ++G EP S + Sbjct 5160322 HFCFLAFPCLRYGSAEPASSSCV 5160390 > KQ557204.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold3, whole genome shotgun sequence Length=33259606 Score = 104 bits (259), Expect = 3e-24, Method: Composition-based stats. Identities = 45/87 (52%), Positives = 61/87 (70%), Gaps = 0/87 (0%) Frame = +3 Query 1 QVSLAVNVASECGYTDEHYKDLQQLQKDFGPFHFNVLAFPCNQFGQQEPGSDKEIDSFVR 60 Q SL VNVAS C +T+ YK LQ+L ++ G HFNVLAFPC QFG E G ++I++F + Sbjct 24957945 QASLVVNVASHCQHTEASYKSLQELHRELGTSHFNVLAFPCGQFGDTERGLSRDIEAFAK 24958124 Query 61 RVYGVSFPIFSKIAVVGIGANNAYKYL 87 YGV+FP FSKI ++G A A+++L Sbjct 24958125 STYGVTFPFFSKIKILGTEAEPAFRFL 24958205 Score = 45.4 bits (106), Expect = 9e-05, Method: Compositional matrix adjust. Identities = 17/45 (38%), Positives = 32/45 (71%), Gaps = 0/45 (0%) Frame = +2 Query 103 EASRKEPTWNFWKYLIDTDGKVVDAWGPEVSVKEIRPRITEMVRK 147 ++ +K P WNFWK+L++ +GKV+ W + S++ +R +T +VR+ Sbjct 24959402 DSVQKIPKWNFWKFLVNQEGKVIRFWKTDESMESVRKEVTALVRE 24959536 > KQ557216.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold15, whole genome shotgun sequence Length=30170098 Score = 45.4 bits (106), Expect = 8e-05, Method: Compositional matrix adjust. Identities = 18/39 (46%), Positives = 25/39 (64%), Gaps = 0/39 (0%) Frame = -3 Query 22 LQQLQKDFGPFHFNVLAFPCNQFGQQEPGSDKEIDSFVR 60 L L ++ P +L FPCNQFG+QEPG+ EI S ++ Sbjct 21050366 LNALHEELKPLGLTILGFPCNQFGKQEPGTKSEILSGLK 21050250 Score = 32.0 bits (71), Expect = 3.2, Method: Compositional matrix adjust. Identities = 13/28 (46%), Positives = 17/28 (61%), Gaps = 0/28 (0%) Frame = -2 Query 111 WNFWKYLIDTDGKVVDAWGPEVSVKEIR 138 WNF K+L+ DGK V W P V + +R Sbjct 21048810 WNFEKFLVGPDGKAVMRWNPRVDMSTVR 21048727 > KQ557211.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold10, whole genome shotgun sequence Length=26077971 Score = 44.3 bits (103), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 27/64 (42%), Positives = 36/64 (56%), Gaps = 3/64 (5%) Frame = -1 Query 1 QVSLAVNVASECGYTDEHYKDLQQLQKDFGPFHFNVLAFPCNQFGQQEPGSDKEIDSFVR 60 +V L NVAS G T + +L QLQ + P VL FPCNQFG Q S+ E++ F+ Sbjct 9655323 RVVLIENVASL*GTTARDFSELNQLQSKY-PHRLVVLGFPCNQFGYQV--SNSEVNLFIH 9655153 Query 61 RVYG 64 + G Sbjct 9655152 FLEG 9655141 > KQ557202.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold1, whole genome shotgun sequence Length=25629008 Score = 41.6 bits (96), Expect = 0.002, Method: Compositional matrix adjust. Identities = 17/34 (50%), Positives = 20/34 (59%), Gaps = 0/34 (0%) Frame = -1 Query 17 EHYKDLQQLQKDFGPFHFNVLAFPCNQFGQQEPG 50 Y + L + FG +F VL FPCNQFG Q PG Sbjct 17126096 SQYNRMNALMEMFGNLNFTVLGFPCNQFGLQSPG 17125995 > KQ557213.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold12, whole genome shotgun sequence Length=28566418 Score = 37.4 bits (85), Expect = 0.036, Method: Composition-based stats. Identities = 19/47 (40%), Positives = 23/47 (49%), Gaps = 0/47 (0%) Frame = +3 Query 1 QVSLAVNVASECGYTDEHYKDLQQLQKDFGPFHFNVLAFPCNQFGQQ 47 +V L NVAS G T Y + +L F +L PCNQFG Q Sbjct 15207162 KVVLVENVASL*GTTVRDYTQMNELHSRFSAEGLVILGVPCNQFGHQ 15207302 Score = 31.6 bits (70), Expect = 4.3, Method: Compositional matrix adjust. Identities = 21/91 (23%), Positives = 41/91 (45%), Gaps = 9/91 (10%) Frame = +3 Query 66 SFPIFSKIAVVGIGANNAYKYL---------GLLSSKHSSVFLLISEASRKEPTWNFWKY 116 +F + K+ V G A+ + YL +S + F++ S R + +WNF K+ Sbjct 15207738 NFQLLEKVDVNGSEAHPLFVYLKEKLPVPSDNAVSLMNDPKFIIWSPVCRSDISWNFEKF 15207917 Query 117 LIDTDGKVVDAWGPEVSVKEIRPRITEMVRK 147 LI DG+ + ++ I E++++ Sbjct 15207918 LIGADGEPYKRYSRNFLTIDLEGDIKELLKR 15208010 > KQ557217.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold16, whole genome shotgun sequence Length=12713041 Score = 31.6 bits (70), Expect = 4.5, Method: Compositional matrix adjust. Identities = 14/33 (42%), Positives = 18/33 (55%), Gaps = 2/33 (6%) Frame = +2 Query 12 CGYTDEHYKDLQQLQKDFGPFHFNVLAFPCNQF 44 CG++ YK QQ + GP NV FP N+F Sbjct 12578450 CGHSS--YKQAQQKRHKHGPLRLNVPHFPLNEF 12578542 Lambda K H a alpha 0.320 0.137 0.417 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 8736787023 Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f a Posted date: Oct 21, 2016 4:48 PM Number of letters in database: 708,396,389 Number of sequences in database: 25 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40