TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f
a
           25 sequences; 708,396,389 total letters



Query= SPP00000614_2.0 # Protein # Iodothyronine deiodinase 2 (DIO2) #
Zebrafish

Length=257
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  KQ557211.1 Xiphophorus couchianus unplaced genomic scaffold Sca...    282   4e-84


> KQ557211.1 Xiphophorus couchianus unplaced genomic scaffold Scaffold10, 
whole genome shotgun sequence
Length=26077971

 Score =   282 bits (721),  Expect = 4e-84, Method: Compositional matrix adjust.
 Identities = 140/192 (73%), Positives = 157/192 (82%), Gaps = 8/192 (4%)
 Frame = -2

Query  73       QVKLGEAAPNSKVVKVTGINRCWSISG-------KTHNQCHLLDFESPDRPLVVNFGSAT  125
                QVKLG  APNSKVVKV    R WS +        +T  +C LLDFES DRPLVVNFGSAT
Sbjct  7723130  QVKLGCEAPNSKVVKVPDGPR-WSSTVVPYGSRIQTGGECRLLDFESSDRPLVVNFGSAT  7722954

Query  126      XPPFISQLPVFRRMVEEFSDVADFLLVYIDEAHPSDGWVGPPMENFSFEVRKHRNLEERM  185
                 PPFIS LP FR++VE+FSDVADFLLVYIDEAHP+DGWV PPM   SF  RKH+NLEER+
Sbjct  7722953  *PPFISHLPAFRQLVEDFSDVADFLLVYIDEAHPADGWVAPPMGPCSFSFRKHQNLEERI  7722774

Query  186      FAARTLLEHFSLPPQCQLVADCMDNNANIAYGVSYERVCIVQKNKIAYLGGKGPFFYNLK  245
                 AAR L++HFSLPPQCQLVADCMDNNAN+AYGV+ ERVCIV + KIAYLGGKGPFFYNLK
Sbjct  7722773  GAARQLIDHFSLPPQCQLVADCMDNNANVAYGVANERVCIVHQRKIAYLGGKGPFFYNLK  7722594

Query  246      DVRRWLEKCYGK  257
                DVR+WLE+ YG+
Sbjct  7722593  DVRQWLEQSYGR  7722558


 Score =   154 bits (388),  Expect = 8e-40, Method: Compositional matrix adjust.
 Identities = 83/252 (33%), Positives = 144/252 (57%), Gaps = 25/252 (10%)
 Frame = +3

Query  10       VTLQILPGFFSNCLFFVLYDSIVLVKRVVSLLSCSGSTG--------EWQRMLTTAGVRS  61
                V   +LP F    + F L D + + KR    +   G           +  R+ +   +++
Sbjct  3441957  VCFVLLPRFLMAAVVFWLLDFLCIRKRFFFRVKEQGGDAIDPPVCISDSNRLFSLESLKA  3442136

Query  62       IWNSFLLDAYKQVKLGEAAPNSKVVKVTGINRCWSISGKTHNQCHLLDFESPDRPLVVNF  121
                +W+   LD  K   LG  APN++VV+         +  + H++  +LDF   DRPL++NF
Sbjct  3442137  VWHGQKLDFLKAAHLGRGAPNTEVVR---------LEDRQHSR--ILDFAHGDRPLILNF  3442283

Query  122      GSATXPPFISQLPVFRRMVEEFSDVADFLLVYIDEAHPSDGWVGPPMENFSFEVRKHRNL  181
                GS T PPF+++L  F+ + +  +D+AD ++VYI+EAHPSDGW      +  +++  H++L
Sbjct  3442284  GSCT*PPFMARLKAFQEVAQLNADIADTVVVYIEEAHPSDGWTS---TDAPYQIPTHQSL  3442454

Query  182      EERMFAARTLLEHFSLPPQCQLVADCMDNNANIAYGVSYERVCIVQKNKIAYLGGKGPFF  241
                EER+ AA   L H  + P C++VAD M+++++ AYG  + R+ ++ +  +AY GG+GP  
Sbjct  3442455  EERLSAAH--LIHLEV-PGCRVVADNMEDSSSAAYGAYFNRLYVLHRGTVAYQGGRGPEG  3442625

Query  242      YNLKDVRRWLEK  253
                Y + ++R WL++
Sbjct  3442626  YRVSELRAWLDQ  3442661


 Score =   119 bits (299),  Expect = 4e-28, Method: Compositional matrix adjust.
 Identities = 61/78 (78%), Positives = 67/78 (86%), Gaps = 2/78 (3%)
 Frame = -1

Query  1        MGLLSVDLLVTLQILPGFFSNCLFFVLYDSIVLVKRVVSLLSCSGST--GEWQRMLTTAG  58
                MG  S DLLVTLQILPGFFSNCLF  LYDS+VLVKRVV+LLS S S   GEW+RMLT+ G
Sbjct  7727262  MGSASEDLLVTLQILPGFFSNCLFLALYDSVVLVKRVVTLLSRSHSAGCGEWRRMLTSEG  7727083

Query  59       VRSIWNSFLLDAYKQVKL  76
                +RSIWNSFLLDAYKQV+L
Sbjct  7727082  LRSIWNSFLLDAYKQVRL  7727029



Lambda      K        H        a         alpha
   0.324    0.140    0.440    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 32585823252


  Database: /export/cursos/20428/BI/genomes/2016/Xiphophorus_couchianus/genome.f
a
    Posted date:  Oct 21, 2016  4:48 PM
  Number of letters in database: 708,396,389
  Number of sequences in database:  25



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40