TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /export/cursos/20428/BI/genomes/2016/Manis_javanica/genome.fa 80,669 sequences; 2,547,379,330 total letters Query= SPP00000067_2.0 # Protein # 15 kDa selenoprotein (Sel15) # Human Length=162 Score E Sequences producing significant alignments: (Bits) Value JSZB01012017.1 Manis javanica isolate MP_PG03-UM SCAFFOLD14246,... 88.2 5e-18 JSZB01034099.1 Manis javanica isolate MP_PG03-UM SCAFFOLD45619,... 43.9 2e-04 JSZB01003378.1 Manis javanica isolate MP_PG03-UM SCAFFOLD3795, ... 33.5 4.5 > JSZB01012017.1 Manis javanica isolate MP_PG03-UM SCAFFOLD14246, whole genome shotgun sequence Length=130207 Score = 88.2 bits (217), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 42/45 (93%), Positives = 45/45 (100%), Gaps = 0/45 (0%) Frame = -3 Query 118 IKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI 162 ++YVRGSDPVLKLLDD+GNIAEELSILKWNTDSVEEFLSEKLERI Sbjct 41597 LQYVRGSDPVLKLLDDSGNIAEELSILKWNTDSVEEFLSEKLERI 41463 Score = 88.2 bits (217), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 56/62 (90%), Positives = 58/62 (94%), Gaps = 0/62 (0%) Frame = -3 Query 26 VSAFGAEFSSEACRELGFssnllcsscdllGQFNLLQLDPDCRGCCQEEAQFETKKLYAG 85 VSA GAEFSSEACRELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKK+ Sbjct 77783 VSALGAEFSSEACRELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKKVLFS 77604 Query 86 AI 87 +I Sbjct 77603 SI 77598 Score = 43.1 bits (100), Expect = 0.002, Method: Compositional matrix adjust. Identities = 23/34 (68%), Positives = 25/34 (74%), Gaps = 1/34 (3%) Frame = -2 Query 77 FETKKLYAGAILEVCGXKLGRFPQVQAF-VRSDK 109 F +LYAGAILEVCG KLGRFPQVQ + S K Sbjct 55695 FFHLQLYAGAILEVCG*KLGRFPQVQGIKINSSK 55594 Score = 37.4 bits (85), Expect = 0.20, Method: Compositional matrix adjust. Identities = 17/17 (100%), Positives = 17/17 (100%), Gaps = 0/17 (0%) Frame = -2 Query 103 AFVRSDKPKLFRGLQIK 119 AFVRSDKPKLFRGLQIK Sbjct 44397 AFVRSDKPKLFRGLQIK 44347 > JSZB01034099.1 Manis javanica isolate MP_PG03-UM SCAFFOLD45619, whole genome shotgun sequence Length=316706 Score = 43.9 bits (102), Expect(3) = 2e-04, Method: Compositional matrix adjust. Identities = 32/95 (34%), Positives = 42/95 (44%), Gaps = 19/95 (20%) Frame = -2 Query 56 GQFNLLQLDPDCRGCCQEEAQFETKKLYAGAILEVCGXKLGRFPQVQAFVR--SDKPKLF 113 GQF L Q DPDCR CQ E + +G + K G+FP + R + +F Sbjct 56656 GQFILFQPDPDCREYCQREVPLQDVGCRSGNLF----TKPGKFPSIHDSSRGLQNMSNMF 56489 Query 114 RGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNT 148 L K + GN+AEEL+I K NT Sbjct 56488 EILSTKAL-------------GNMAEELNIFK*NT 56423 Score = 22.7 bits (47), Expect(3) = 2e-04, Method: Compositional matrix adjust. Identities = 10/12 (83%), Positives = 11/12 (92%), Gaps = 0/12 (0%) Frame = -1 Query 151 VEEFLSEKLERI 162 VEEFLSEKLE + Sbjct 56420 VEEFLSEKLEHM 56385 Score = 18.5 bits (36), Expect(3) = 2e-04, Method: Compositional matrix adjust. Identities = 15/22 (68%), Positives = 18/22 (82%), Gaps = 0/22 (0%) Frame = -1 Query 11 PAFGLRLLLATVLQAVSAFGAE 32 PAF LRLLLAT + A+SAFG + Sbjct 56780 PAFRLRLLLATAV*ALSAFGGQ 56715 > JSZB01003378.1 Manis javanica isolate MP_PG03-UM SCAFFOLD3795, whole genome shotgun sequence Length=811246 Score = 33.5 bits (75), Expect = 4.5, Method: Compositional matrix adjust. Identities = 16/38 (42%), Positives = 25/38 (66%), Gaps = 1/38 (3%) Frame = +3 Query 68 RGCCQEEAQFETKKLYAGAILEVCGXKLGRFPQVQAFV 105 +GCC++E++ + + A A L CG KLG+FP + A V Sbjct 805758 QGCCEKESEHASDQEVA-ARLPGCGSKLGKFPHLSASV 805868 Lambda K H a alpha 0.322 0.140 0.416 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 35256738846 Database: /export/cursos/20428/BI/genomes/2016/Manis_javanica/genome.fa Posted date: Oct 21, 2016 4:42 PM Number of letters in database: 2,547,379,330 Number of sequences in database: 80,669 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40