TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /export/cursos/20428/BI/genomes/2016/Manis_javanica/genome.fa 80,669 sequences; 2,547,379,330 total letters Query= SPP00000116_2.0 # Protein # Selenoprotein U3 (SELENOU3) # Human Length=228 Score E Sequences producing significant alignments: (Bits) Value JSZB01006448.1 Manis javanica isolate MP_PG03-UM SCAFFOLD7381, ... 45.8 0.001 JSZB01001214.1 Manis javanica isolate MP_PG03-UM SCAFFOLD1338, ... 41.2 0.032 > JSZB01006448.1 Manis javanica isolate MP_PG03-UM SCAFFOLD7381, whole genome shotgun sequence Length=120346 Score = 45.8 bits (107), Expect = 0.001, Method: Compositional matrix adjust. Identities = 24/36 (67%), Positives = 26/36 (72%), Gaps = 0/36 (0%) Frame = +1 Query 85 SSLAGLLDQHGVRLVGVGPEALGLQEFLDGDYFAGE 120 SSL L DQHGV LV + EA L+EFLDGDYFAG Sbjct 43813 SSLRSLRDQHGVCLVRLWCEAPHLREFLDGDYFAGN 43920 Score = 43.1 bits (100), Expect = 0.006, Method: Compositional matrix adjust. Identities = 23/38 (61%), Positives = 25/38 (66%), Gaps = 4/38 (11%) Frame = +2 Query 66 VAGLRRFGCVVC----RWIAQDLSSLAGLLDQHGVRLV 99 V GLR FGC+ RW+A DLSSL L DQHGV LV Sbjct 43697 VXGLRSFGCMWSPPRSRWVAGDLSSLRSLRDQHGVCLV 43810 Score = 39.3 bits (90), Expect = 0.11, Method: Compositional matrix adjust. Identities = 18/24 (75%), Positives = 20/24 (83%), Gaps = 0/24 (0%) Frame = +3 Query 52 AVELRSLWREHACVVAGLRRFGCV 75 AVELRSLW+E A V AGLR FGC+ Sbjct 43611 AVELRSLWQEQARVAAGLRSFGCM 43682 > JSZB01001214.1 Manis javanica isolate MP_PG03-UM SCAFFOLD1338, whole genome shotgun sequence Length=551697 Score = 41.2 bits (95), Expect = 0.032, Method: Compositional matrix adjust. Identities = 21/51 (41%), Positives = 29/51 (57%), Gaps = 0/51 (0%) Frame = -3 Query 56 RSLWREHACVVAGLRRFGCVVCRWIAQDLSSLAGLLDQHGVRLVGVGPEAL 106 + LW + V+ + R GC +CR A+DLSSL LD+ GV L V E + Sbjct 239797 KELWEKSGAVIMAVWRPGCFLCREEAEDLSSLKPKLDELGVPLYAVVKEQI 239645 Lambda K H a alpha 0.319 0.136 0.407 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 85574139198 Database: /export/cursos/20428/BI/genomes/2016/Manis_javanica/genome.fa Posted date: Oct 21, 2016 4:42 PM Number of letters in database: 2,547,379,330 Number of sequences in database: 80,669 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40