TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Manis_javanica/genome.fa
           80,669 sequences; 2,547,379,330 total letters



Query= SPP00000052_2.0 # Protein # Phosphoseryl-tRNA kinase  (PSTK) # Human

Length=348
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JSZB01001613.1 Manis javanica isolate MP_PG03-UM SCAFFOLD1784, ...    186   5e-49
  JSZB01009744.1 Manis javanica isolate MP_PG03-UM SCAFFOLD11401,...  35.8    3.9  


> JSZB01001613.1 Manis javanica isolate MP_PG03-UM SCAFFOLD1784, 
whole genome shotgun sequence
Length=777097

 Score =   186 bits (471),  Expect = 5e-49, Method: Compositional matrix adjust.
 Identities = 89/97 (92%), Positives = 90/97 (93%), Gaps = 0/97 (0%)
 Frame = -1

Query  73      PSQWKLLRQELLKYLEYFLMAVINGCQMSVPPNRTEAMWEDFITCLKDQDLIFSAAFEAQ  132
               PSQWKLLRQELLKYLE FL AVINGCQMS PPNRTEAMWEDFITCLKDQDL+ SAA EAQ
Sbjct  510010  PSQWKLLRQELLKYLEGFLTAVINGCQMSAPPNRTEAMWEDFITCLKDQDLVSSAAHEAQ  509831

Query  133     SCYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARK  169
               SCY LTKTAVSRPL LVLDDNFYYQSMRYEVYQLARK
Sbjct  509830  SCYFLTKTAVSRPLLLVLDDNFYYQSMRYEVYQLARK  509720


 Score =   120 bits (300),  Expect = 5e-27, Method: Compositional matrix adjust.
 Identities = 66/129 (51%), Positives = 78/129 (60%), Gaps = 18/129 (14%)
 Frame = -2

Query  171     SLGFCQLFLDCPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPA  230
               SLGFCQLFLDCPLETCLQRN +RP+ALP +TIHLMG K+EKPNPEKNAWEHNSLTI S  
Sbjct  509478  SLGFCQLFLDCPLETCLQRNSRRPRALPTQTIHLMGSKIEKPNPEKNAWEHNSLTIQSAM  509299

Query  231     CASEASLEV--------TDLLLTALENPVKYAEDNMEQKD----------TDRIICSTNI  272
               C+SEA            + LL+  L NP   A   + Q +            ++ C    
Sbjct  509298  CSSEARYPTQSHQARCSSALLVFHLVNPYASAGGRLLQTE*EVSFVQSHGPQQLRCEPKF  509119

Query  273     LHKTDQTLR  281
               +HK    LR
Sbjct  509118  VHKRVLLLR  509092


 Score =   112 bits (280),  Expect = 1e-24, Method: Compositional matrix adjust.
 Identities = 53/71 (75%), Positives = 61/71 (86%), Gaps = 0/71 (0%)
 Frame = -3

Query  1       MKTAENIRGTGSDGPRKRGLCVLCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVMP  60
               MKT+E++RG  S+GPRK GLCVLCGLPAAGKSTFAR L+HRL+QE  WA+GVVAYDDVMP
Sbjct  512216  MKTSEHVRGARSEGPRKLGLCVLCGLPAAGKSTFARVLSHRLRQEGRWAVGVVAYDDVMP  512037

Query  61      DAFLAGARARP  71
               DAF+  A A P
Sbjct  512036  DAFIKEASAPP  512004


 Score = 60.5 bits (145),  Expect = 7e-08, Method: Compositional matrix adjust.
 Identities = 27/32 (84%), Positives = 30/32 (94%), Gaps = 0/32 (0%)
 Frame = -1

Query  262     DTDRIICSTNILHKTDQTLRRIVSQTMKEAKG  293
               +TDRIICS +ILH+ DQTLRRIVSQTMKEAKG
Sbjct  506326  ETDRIICSASILHQVDQTLRRIVSQTMKEAKG  506231


 Score = 53.1 bits (126),  Expect = 1e-05, Method: Compositional matrix adjust.
 Identities = 29/46 (63%), Positives = 35/46 (76%), Gaps = 3/46 (7%)
 Frame = -1

Query  216     KNAWEHNSLTIPSPACASEASLEVTDLLLTALENPVKYAEDNMEQK  261
               KN ++     IP P+ +S  SL++TDLLLTALENPVKY EDNMEQK
Sbjct  506980  KNMFDVRMYFIP-PSFSS--SLKLTDLLLTALENPVKYVEDNMEQK  506852


 Score = 39.7 bits (91),  Expect = 0.20, Method: Compositional matrix adjust.
 Identities = 19/29 (66%), Positives = 21/29 (72%), Gaps = 3/29 (10%)
 Frame = -1

Query  291     AKGNQEAFSEMTFKQR---WVRANHAAIW  316
               ++GNQE FSEMTFKQR     R NHA IW
Sbjct  505879  SRGNQEGFSEMTFKQRCE*KERTNHANIW  505793


> JSZB01009744.1 Manis javanica isolate MP_PG03-UM SCAFFOLD11401, 
whole genome shotgun sequence
Length=1113149

 Score = 35.8 bits (81),  Expect = 3.9, Method: Compositional matrix adjust.
 Identities = 28/105 (27%), Positives = 49/105 (47%), Gaps = 9/105 (9%)
 Frame = +3

Query  170   YSLGFCQLFLDCPLETC--LQRNGQR-----PQALPPETIHLMGRKLEKPNPEKNAWEHN  222
             YSLGF  + L C +  C  L  +G       PQ+L P+++H   +      P      + 
Sbjct  6168  YSLGFQGITLSCLISDCFDLTFSGFHLFFG*PQSLVPDSLHFS*QPFSNQ*PHLILCLYI  6347

Query  223   SLTIPSPACASEASLEVTDLLLTALENPVKYAEDNMEQKDTDRII  267
             S  I SP   S   +++++  LT+L    ++   + EQ++ D +I
Sbjct  6348  SCLISSPKLFSGL*IQISNYTLTSLGG--RFTIISTEQEEIDSLI  6476



Lambda      K        H        a         alpha
   0.321    0.134    0.417    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 181967260468


  Database: /export/cursos/20428/BI/genomes/2016/Manis_javanica/genome.fa
    Posted date:  Oct 21, 2016  4:42 PM
  Number of letters in database: 2,547,379,330
  Number of sequences in database:  80,669



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40