TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Manis_javanica/genome.fa
           80,669 sequences; 2,547,379,330 total letters



Query= SPP00000008_2.0 # Protein # Iodothyronine deiodinase 2 (DIO2) #
Human

Length=273
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JSZB01004262.1 Manis javanica isolate MP_PG03-UM SCAFFOLD4833, ...    358   9e-110
  JSZB01002288.1 Manis javanica isolate MP_PG03-UM SCAFFOLD2556, ...    157   2e-40 
  JSZB01004892.1 Manis javanica isolate MP_PG03-UM SCAFFOLD5563, ...  60.5    3e-08 
  JSZB01000763.1 Manis javanica isolate MP_PG03-UM SCAFFOLD847, w...  35.4    3.0   


> JSZB01004262.1 Manis javanica isolate MP_PG03-UM SCAFFOLD4833, 
whole genome shotgun sequence
Length=249848

 Score =   358 bits (920),  Expect = 9e-110, Method: Compositional matrix adjust.
 Identities = 174/203 (86%), Positives = 186/203 (92%), Gaps = 6/203 (3%)
 Frame = -2

Query  63      VWKSFLLDAYKQVKLGEDAPNSSVVHVSSTEGGDNSGNGTQEKIAEGATCHLLDFASPER  122
               ++K+F    + QVKLGEDAPNSSVVHVSS EGGDNSG+G QEK+ +GA CHLLDFA PER
Sbjct  176431  LFKNFF---HHQVKLGEDAPNSSVVHVSSREGGDNSGSGAQEKVTDGAECHLLDFAHPER  176261

Query  123     PLVVNFGSATXPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEV  182
               PLVVNFGSAT PPFTSQLPAFR LV+EFSSVADFLLVYIDEAHPSDGWA+P   SLSFEV
Sbjct  176260  PLVVNFGSAT*PPFTSQLPAFRTLVDEFSSVADFLLVYIDEAHPSDGWAVP---SLSFEV  176090

Query  183     KKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLG  242
               KKH+NQEDRCAAAQQLLERFSLPPQC+VVADRMDNNAN+AYGVAFERVCIVQRQKIAYLG
Sbjct  176089  KKHRNQEDRCAAAQQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRQKIAYLG  175910

Query  243     GKGPFSYNLQEVRHWLEKNFSKR  265
               GKGPF YNLQEVR WLEKNFSKR
Sbjct  175909  GKGPFCYNLQEVRCWLEKNFSKR  175841


 Score =   150 bits (380),  Expect = 4e-38, Method: Compositional matrix adjust.
 Identities = 73/75 (97%), Positives = 75/75 (100%), Gaps = 0/75 (0%)
 Frame = -1

Query  1       MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGL  60
               MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEG+
Sbjct  184376  MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGM  184197

Query  61      RCVWKSFLLDAYKQV  75
               RC+WKSFLLDAYKQV
Sbjct  184196  RCIWKSFLLDAYKQV  184152


> JSZB01002288.1 Manis javanica isolate MP_PG03-UM SCAFFOLD2556, 
whole genome shotgun sequence
Length=416281

 Score =   157 bits (397),  Expect = 2e-40, Method: Compositional matrix adjust.
 Identities = 98/273 (36%), Positives = 137/273 (50%), Gaps = 51/273 (19%)
 Frame = +2

Query  12      LQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEG------------  59
               L + P F      L L D + + KH   LL R    + E    LT +G            
Sbjct  210857  LVLFPRFLGTAFMLWLLDFLCIRKH---LLGRGHRGQPEPEVELTGDGEEVPPDDPPVCV  211027

Query  60      -----------LRCVWKSFLLDAYKQVKLGEDAPNSSVVHVSSTEGGDNSGNGTQEKIAE  108
                          L+ VW    LD +KQ   G  APNS VV                  + E
Sbjct  211028  SDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVV------------------LPE  211153

Query  109     G-ATCHLLDFASPERPLVVNFGSATXPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPS  167
               G  + H+LD+A   RPLV+NFGS T PPF +++ AF++LV ++    DFL++YI+EAHPS
Sbjct  211154  GFRSQHILDYARGTRPLVLNFGSCT*PPFMARMSAFQRLVTKYQHDVDFLIIYIEEAHPS  211333

Query  168     DGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAF  227
               DGW     +   + + +H+N EDR  AA+ L +     P C +V D M N+++ AYG  F
Sbjct  211334  DGWVT---TDSPYSIPQHRNLEDRVIAARVLQQGA---PSCSLVLDTMANSSSSAYGAYF  211495

Query  228     ERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEK  260
               ER+ +VQ   I Y GG+GP  Y + E+R WLE+
Sbjct  211496  ERLYVVQSGTIMYQGGRGPDGYQVSELRFWLER  211594


> JSZB01004892.1 Manis javanica isolate MP_PG03-UM SCAFFOLD5563, 
whole genome shotgun sequence
Length=196381

 Score = 60.5 bits (145),  Expect = 3e-08, Method: Compositional matrix adjust.
 Identities = 27/46 (59%), Positives = 36/46 (78%), Gaps = 0/46 (0%)
 Frame = +2

Query  122     RPLVVNFGSATXPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPS  167
               RPLV+NFGS T P F  +   F+ L+E+FSS+ADFL++YI+EAH S
Sbjct  159353  RPLVLNFGSCT*PSFLFKFDQFKGLIEDFSSIADFLVIYIEEAHAS  159490


> JSZB01000763.1 Manis javanica isolate MP_PG03-UM SCAFFOLD847, 
whole genome shotgun sequence
Length=1082010

 Score = 35.4 bits (80),  Expect = 3.0, Method: Compositional matrix adjust.
 Identities = 25/75 (33%), Positives = 35/75 (47%), Gaps = 13/75 (17%)
 Frame = -2

Query  51      WRRMLTSEGLRCVWKSFLLDAYKQVKLGEDAPNSSVVHVSSTEGGDN----SGNGTQEKI  106
               WR  LT+  +RC W  F      Q ++GED   SS++HV S   GDN      N      
Sbjct  703577  WR--LTTPKIRC-WPKF------QSRVGEDQSMSSLMHVCSVNSGDNCHAKQTNKKTNTH  703425

Query  107     AEGATCHLLDFASPE  121
               A+    HL +  +P+
Sbjct  703424  AQKTNKHLFEKINPK  703380



Lambda      K        H        a         alpha
   0.321    0.136    0.406    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 121626117595


  Database: /export/cursos/20428/BI/genomes/2016/Manis_javanica/genome.fa
    Posted date:  Oct 21, 2016  4:42 PM
  Number of letters in database: 2,547,379,330
  Number of sequences in database:  80,669



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40