TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
           6,294 sequences; 619,300,777 total letters



Query= SPP00000655_2.0 # Protein # Selenoprotein U2 (SELENOU2) # Zebrafish

Length=222
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JXSJ01000066.1 Miichthys miiuy scaffold66, whole genome shotgun...  70.9    3e-34


> JXSJ01000066.1 Miichthys miiuy scaffold66, whole genome shotgun 
sequence
Length=1661281

 Score = 70.9 bits (172),  Expect(3) = 3e-34, Method: Compositional matrix adjust.
 Identities = 33/48 (69%), Positives = 38/48 (79%), Gaps = 1/48 (2%)
 Frame = -3

Query  176     LIVGPGPDVHFAHFDMNRLDHMPINWLLQLAGL-PALDFSDSPKIINI  222
               L+   G  VHF+HFD NRLDHMPINWLLQLAG+   LDFSD PKII++
Sbjct  443174  LLTHAGSQVHFSHFDTNRLDHMPINWLLQLAGVQQTLDFSDKPKIIHV  443031


 Score = 67.8 bits (164),  Expect(3) = 3e-34, Method: Compositional matrix adjust.
 Identities = 34/87 (39%), Positives = 51/87 (59%), Gaps = 31/87 (36%)
 Frame = -2

Query  80      VLQDSNVRLVVIGQSSYSHIQ-------------------------------GFCSLTGF  108
               +LQD+++RLVVIGQS++ HI+                                FCSLTG+
Sbjct  443766  LLQDADIRLVVIGQSAHHHIEVIILFYIFSHMHNVSLPHMFPLC*CVFHLI*PFCSLTGY  443587

Query  109     PHEIYVDPERQIYKRLGLRRGETYMET  135
               P+E+YVDPER IY++LG++R ET+ ++
Sbjct  443586  PYEMYVDPERCIYQKLGMKREETFTDS  443506


 Score = 48.5 bits (114),  Expect(3) = 3e-34, Method: Compositional matrix adjust.
 Identities = 35/50 (70%), Positives = 39/50 (78%), Gaps = 2/50 (4%)
 Frame = -1

Query  132     YMETPSVSPHVKSSMLSGSLKSVWRAMTSPVfdfqgdpqqqggALIVGPG  181
               Y+  PS  PHVKS M  G +KS+WRAMTSPVFDFQGD  QQGGA+I GPG
Sbjct  443416  YLAHPS--PHVKSGMFMGQMKSIWRAMTSPVFDFQGDLHQQGGAIIAGPG  443273


 Score = 48.1 bits (113),  Expect = 4e-05, Method: Compositional matrix adjust.
 Identities = 21/55 (38%), Positives = 36/55 (65%), Gaps = 0/55 (0%)
 Frame = -1

Query  5       PITRQISSSVQDNKQASHNICLSELKNCFIFDRHGDKKSFSSLFEHNKAIVIFVR  59
               PITRQ++   ++   +S +I L ++++C I+DRHG    F  L +  K+++IFVR
Sbjct  444802  PITRQVTKENREIGTSSVDIPLKDVEDCIIYDRHGVSIPFKKLHQDRKSVIIFVR  444638


 Score = 45.4 bits (106),  Expect = 3e-04, Method: Composition-based stats.
 Identities = 21/46 (46%), Positives = 32/46 (70%), Gaps = 3/46 (7%)
 Frame = -2

Query  42      KSFSSLFEHNK---AIVIFVRHFLCYTCKEYVEDLGKIPQHVLQDS  84
               KS ++ +EH      + + +++FLCY+CKEYVEDL KIP+  L+ S
Sbjct  444363  KSNATAYEHIICVCTVCVSLQNFLCYSCKEYVEDLSKIPKEALEVS  444226



Lambda      K        H        a         alpha
   0.323    0.137    0.422    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 21804569728


  Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
    Posted date:  Oct 21, 2016  4:44 PM
  Number of letters in database: 619,300,777
  Number of sequences in database:  6,294



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40