TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
           6,294 sequences; 619,300,777 total letters



Query= SPP00000650_2.0 # Protein # Selenoprotein T2 (SELENOT2) # Zebrafish

Length=210
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JXSJ01000199.1 Miichthys miiuy scaffold199, whole genome shotgu...  64.3    3e-21
  JXSJ01000006.1 Miichthys miiuy scaffold6, whole genome shotgun ...  50.8    5e-14


> JXSJ01000199.1 Miichthys miiuy scaffold199, whole genome shotgun 
sequence
Length=504515

 Score = 64.3 bits (155),  Expect(2) = 3e-21, Method: Compositional matrix adjust.
 Identities = 25/44 (57%), Positives = 35/44 (80%), Gaps = 0/44 (0%)
 Frame = +1

Query  99      KYLGNFLSYFKLLAIALIVTGQNPFQMFGMNTPRIWAWGQENKI  142
               + +GN +SY KLL+I LIV+GQNPF + G++TPR W W Q+NK+
Sbjct  221095  RCVGNLVSYLKLLSILLIVSGQNPFILLGLDTPRAWTWSQDNKV  221226


 Score = 58.9 bits (141),  Expect(2) = 3e-21, Method: Compositional matrix adjust.
 Identities = 25/31 (81%), Positives = 29/31 (94%), Gaps = 0/31 (0%)
 Frame = +2

Query  141     KIFSCLMAFFISNMLETHFLSTGAFEITLND  171
               +IFSCLMAFF+ NM+ETHFLSTGAFE+TLN 
Sbjct  221294  QIFSCLMAFFLCNMMETHFLSTGAFEVTLNG  221386


 Score = 79.0 bits (193),  Expect = 4e-15, Method: Compositional matrix adjust.
 Identities = 37/52 (71%), Positives = 43/52 (83%), Gaps = 4/52 (8%)
 Frame = +2

Query  158     HFLSTGAFEITLNDIPIWSKLQSGYVPNIQELFQILDNHLKMNQADKMNFPS  209
               HFL    F + L+D+P+WSKLQSGYVPNIQE+FQILDNHLKMNQ D M+F S
Sbjct  221690  HFL----FPVCLSDVPLWSKLQSGYVPNIQEIFQILDNHLKMNQVDPMSFTS  221833


 Score = 69.3 bits (168),  Expect = 3e-12, Method: Compositional matrix adjust.
 Identities = 33/45 (73%), Positives = 40/45 (89%), Gaps = 1/45 (2%)
 Frame = +1

Query  61      YCISXGYSKVFQEYSRSISQLYPDIRIEGDNYPPKPINKYLGNFL  105
               + IS GYSKVFQEYSR+ISQLYPDIRIEG+NYPP P N+++ N+L
Sbjct  220687  FSIS*GYSKVFQEYSRAISQLYPDIRIEGENYPPTPFNRWV-NYL  220818


 Score = 44.7 bits (104),  Expect = 4e-04, Method: Compositional matrix adjust.
 Identities = 37/72 (51%), Positives = 46/72 (64%), Gaps = 11/72 (15%)
 Frame = +3

Query  1       MAEYSQTGIltalllftvvtvKDIYVGRNSV--TQQENTGP---------DINTQRQNKH  49
               MAEYSQ G+L ALLLFT +TV+D+Y+GR+S+   QQ   GP         D    +  K 
Sbjct  220221  MAEYSQAGLLAALLLFTALTVRDVYLGRSSLHRGQQPADGPSLSPDTVLPDTEPGKPTKS  220400

Query  50      TFYTGPVLKFQY  61
               + YTGPVLKFQY
Sbjct  220401  SLYTGPVLKFQY  220436


> JXSJ01000006.1 Miichthys miiuy scaffold6, whole genome shotgun 
sequence
Length=7996890

 Score = 50.8 bits (120),  Expect(2) = 5e-14, Method: Compositional matrix adjust.
 Identities = 19/30 (63%), Positives = 28/30 (93%), Gaps = 0/30 (0%)
 Frame = -1

Query  171      DIPIWSKLQSGYVPNIQELFQILDNHLKMN  200
                D+P+WSKL+SG++P++Q+L QILDN +KMN
Sbjct  6154557  DVPVWSKLESGHLPSMQQLVQILDNEMKMN  6154468


 Score = 47.8 bits (112),  Expect(2) = 5e-14, Method: Compositional matrix adjust.
 Identities = 19/30 (63%), Positives = 26/30 (87%), Gaps = 0/30 (0%)
 Frame = -3

Query  141      KIFSCLMAFFISNMLETHFLSTGAFEITLN  170
                +I++C+M FF+SNM+E   +STGAFEITLN
Sbjct  6154759  QIYACMMVFFLSNMIENQLMSTGAFEITLN  6154670


 Score = 60.5 bits (145),  Expect = 3e-09, Method: Composition-based stats.
 Identities = 23/44 (52%), Positives = 33/44 (75%), Gaps = 0/44 (0%)
 Frame = -3

Query  99       KYLGNFLSYFKLLAIALIVTGQNPFQMFGMNTPRIWAWGQENKI  142
                +++ +FLS FKLL I +I+ G++PF +FGM  P IW WGQ NK+
Sbjct  6155926  RHIASFLSMFKLLVIGVIIIGKDPFALFGMQAPGIWEWGQGNKV  6155795


 Score = 53.1 bits (126),  Expect = 7e-07, Method: Compositional matrix adjust.
 Identities = 22/39 (56%), Positives = 31/39 (79%), Gaps = 0/39 (0%)
 Frame = -1

Query  61       YCISXGYSKVFQEYSRSISQLYPDIRIEGDNYPPKPINK  99
                Y IS GY +VF+EY++++ Q YPDIRIEG+NY P P+ +
Sbjct  6157089  YSIS*GYKRVFEEYTQALYQRYPDIRIEGENYLPIPLYR  6156973



Lambda      K        H        a         alpha
   0.321    0.137    0.421    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 19542429290


  Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
    Posted date:  Oct 21, 2016  4:44 PM
  Number of letters in database: 619,300,777
  Number of sequences in database:  6,294



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40