TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
           6,294 sequences; 619,300,777 total letters



Query= SPP00000649_2.0 # Protein # Selenoprotein T1 (SELENOT1) # Zebrafish

Length=178
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JXSJ01000006.1 Miichthys miiuy scaffold6, whole genome shotgun ...  58.5    5e-17
  JXSJ01000199.1 Miichthys miiuy scaffold199, whole genome shotgu...  43.5    8e-09


> JXSJ01000006.1 Miichthys miiuy scaffold6, whole genome shotgun 
sequence
Length=7996890

 Score = 58.5 bits (140),  Expect(2) = 5e-17, Method: Compositional matrix adjust.
 Identities = 27/30 (90%), Positives = 28/30 (93%), Gaps = 0/30 (0%)
 Frame = -3

Query  126      KIYACMMVFFFSNMIENQCMSTGAFEITLN  155
                +IYACMMVFF SNMIENQ MSTGAFEITLN
Sbjct  6154759  QIYACMMVFFLSNMIENQLMSTGAFEITLN  6154670


 Score = 49.7 bits (117),  Expect(2) = 5e-17, Method: Compositional matrix adjust.
 Identities = 23/23 (100%), Positives = 23/23 (100%), Gaps = 0/23 (0%)
 Frame = -1

Query  156      DVPVWSKLESGHLPSMQQLVQIL  178
                DVPVWSKLESGHLPSMQQLVQIL
Sbjct  6154557  DVPVWSKLESGHLPSMQQLVQIL  6154489


 Score = 76.6 bits (187),  Expect = 1e-14, Method: Compositional matrix adjust.
 Identities = 34/37 (92%), Positives = 36/37 (97%), Gaps = 0/37 (0%)
 Frame = -1

Query  48       VSXGYKRVFEEYTQALYQRYPDIRIEGENYLPLPLYR  84
                +S GYKRVFEEYTQALYQRYPDIRIEGENYLP+PLYR
Sbjct  6157083  IS*GYKRVFEEYTQALYQRYPDIRIEGENYLPIPLYR  6156973


 Score = 63.2 bits (152),  Expect = 3e-10, Method: Compositional matrix adjust.
 Identities = 37/44 (84%), Positives = 40/44 (91%), Gaps = 0/44 (0%)
 Frame = -3

Query  84       RHIASFLSMFKllligviilgKDPFALCGMQAPGIWVWSQENKI  127
                RHIASFLSMFKLL+IGVII+GKDPFAL GMQAPGIW W Q NK+
Sbjct  6155926  RHIASFLSMFKLLVIGVIIIGKDPFALFGMQAPGIWEWGQGNKV  6155795


 Score = 58.5 bits (140),  Expect = 7e-09, Method: Compositional matrix adjust.
 Identities = 32/45 (71%), Positives = 37/45 (82%), Gaps = 3/45 (7%)
 Frame = -3

Query  4        MRWLPFS--ALLLWALCLHSASADNNGVKKMKMQFATGPLLKFQI  46
                MRWL FS  AL L++LC  + S D++GVKKMKMQFATGPLLKFQI
Sbjct  6158134  MRWLRFSLLALGLFSLCC-ATSGDSSGVKKMKMQFATGPLLKFQI  6158003


> JXSJ01000199.1 Miichthys miiuy scaffold199, whole genome shotgun 
sequence
Length=504515

 Score = 43.5 bits (101),  Expect(2) = 8e-09, Method: Composition-based stats.
 Identities = 17/30 (57%), Positives = 24/30 (80%), Gaps = 0/30 (0%)
 Frame = +2

Query  126     KIYACMMVFFFSNMIENQCMSTGAFEITLN  155
               +I++C+M FF  NM+E   +STGAFE+TLN
Sbjct  221294  QIFSCLMAFFLCNMMETHFLSTGAFEVTLN  221383


 Score = 36.6 bits (83),  Expect(2) = 8e-09, Method: Composition-based stats.
 Identities = 21/53 (40%), Positives = 33/53 (62%), Gaps = 0/53 (0%)
 Frame = +1

Query  80      LPLYRHIASFLSMFKllligviilgKDPFALCGMQAPGIWVWSQENKIYACMM  132
               L + R + + +S  KLL I +I+ G++PF L G+  P  W WSQ+NK+   M+
Sbjct  221083  LCVLRCVGNLVSYLKLLSILLIVSGQNPFILLGLDTPRAWTWSQDNKVPVTMV  221241


 Score = 55.5 bits (132),  Expect = 6e-08, Method: Composition-based stats.
 Identities = 28/49 (57%), Positives = 34/49 (69%), Gaps = 2/49 (4%)
 Frame = +1

Query  38      TGPLLKFQICVSXGYKRVFEEYTQALYQRYPDIRIEGENYLPLPLYRHI  86
               TG L  F I  S GY +VF+EY++A+ Q YPDIRIEGENY P P  R +
Sbjct  220669  TGVLCVFSI--S*GYSKVFQEYSRAISQLYPDIRIEGENYPPTPFNRWV  220809


 Score = 41.2 bits (95),  Expect = 0.003, Method: Compositional matrix adjust.
 Identities = 16/29 (55%), Positives = 26/29 (90%), Gaps = 0/29 (0%)
 Frame = +2

Query  150     FEITLNDVPVWSKLESGHLPSMQQLVQIL  178
               F + L+DVP+WSKL+SG++P++Q++ QIL
Sbjct  221699  FPVCLSDVPLWSKLQSGYVPNIQEIFQIL  221785



Lambda      K        H        a         alpha
   0.326    0.138    0.447    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 13371954050


  Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
    Posted date:  Oct 21, 2016  4:44 PM
  Number of letters in database: 619,300,777
  Number of sequences in database:  6,294



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40