TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
           6,294 sequences; 619,300,777 total letters



Query= SPP00000631_2.0 # Protein # Selenocysteine synthase (SecS) #
Zebrafish

Length=490
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JXSJ01000055.1 Miichthys miiuy scaffold55, whole genome shotgun...    112   2e-35


> JXSJ01000055.1 Miichthys miiuy scaffold55, whole genome shotgun 
sequence
Length=2100046

 Score =   112 bits (280),  Expect(2) = 2e-35, Method: Compositional matrix adjust.
 Identities = 52/54 (96%), Positives = 52/54 (96%), Gaps = 0/54 (0%)
 Frame = +3

Query  38       QGKCPEEGWSESTIELFLNELAVMDSNNFLGNCGVGEREGRVASSLVARRHYRL  91
                QGKCPEEGWSESTIELFLNELAVMDSNNFL NCGVGEREGRVASSLVARRHYR 
Sbjct  1602855  QGKCPEEGWSESTIELFLNELAVMDSNNFLSNCGVGEREGRVASSLVARRHYRF  1603016


 Score = 60.5 bits (145),  Expect(2) = 2e-35, Method: Compositional matrix adjust.
 Identities = 28/41 (68%), Positives = 32/41 (78%), Gaps = 0/41 (0%)
 Frame = +1

Query  1        MNSENFRLSEKLVSASYVRQGLDARRGHEQLIRRLLDQGKC  41
                MN ENF LSEK+VS +Y+RQG  ARR HEQLIR LL+Q K 
Sbjct  1602664  MNGENFSLSEKIVSPAYIRQGSQARRSHEQLIRHLLEQVKS  1602786


 Score =   114 bits (286),  Expect = 2e-25, Method: Compositional matrix adjust.
 Identities = 50/78 (64%), Positives = 63/78 (81%), Gaps = 0/78 (0%)
 Frame = +3

Query  129      TGVRSVSSCFVVPMATGMSLTLCFLTLRHRRPKARYILWPRIDQKSCFKSMVTAGFEPVV  188
                TGV+ V+SCFVVPMATGMSLTLCFLTLRHRRPKARYI+WPRIDQKSCFK+M+TAG + + 
Sbjct  1609173  TGVQRVASCFVVPMATGMSLTLCFLTLRHRRPKARYIIWPRIDQKSCFKAMITAGDDHIS  1609352

Query  189      IENVLEGDELRTNLEEVE  206
                +       ++  N++ + 
Sbjct  1609353  VRQKKNKIQVYLNVDNMS  1609406


 Score = 91.3 bits (225),  Expect = 7e-18, Method: Compositional matrix adjust.
 Identities = 39/49 (80%), Positives = 46/49 (94%), Gaps = 0/49 (0%)
 Frame = +2

Query  403      ARVVPLGVQQTVSGHTFSGFMSHSEAYPCPYLNAASAIGITKGDVTVCM  451
                +RV+PLG +QTVSGHTF GFMSHSEAYPCPYLNAASA+GIT+ DVT+C+
Sbjct  1609799  SRVIPLGKEQTVSGHTFRGFMSHSEAYPCPYLNAASAVGITREDVTLCI  1609945


 Score = 80.9 bits (198),  Expect = 2e-14, Method: Compositional matrix adjust.
 Identities = 39/46 (85%), Positives = 44/46 (96%), Gaps = 0/46 (0%)
 Frame = +2

Query  90       RLIHGIGRSGDIAAVQPKAAGSSLLNKITNSVVLDVLKLTGVRSVS  135
                RLIHGIGRSGDIAAVQPKAAGSSLLNK+TNSVVLD+LKL+G+  +S
Sbjct  1608599  RLIHGIGRSGDIAAVQPKAAGSSLLNKLTNSVVLDILKLSGMYMLS  1608736


 Score = 80.5 bits (197),  Expect = 2e-14, Method: Compositional matrix adjust.
 Identities = 39/46 (85%), Positives = 44/46 (96%), Gaps = 0/46 (0%)
 Frame = +1

Query  90       RLIHGIGRSGDIAAVQPKAAGSSLLNKITNSVVLDVLKLTGVRSVS  135
                RLIHGIGRSGDIAAVQPKAAGSSLLNK+TNSVVLD+LKL+G+  +S
Sbjct  1603318  RLIHGIGRSGDIAAVQPKAAGSSLLNKLTNSVVLDILKLSGMYMLS  1603455


 Score = 50.4 bits (119),  Expect = 4e-05, Method: Compositional matrix adjust.
 Identities = 25/42 (60%), Positives = 30/42 (71%), Gaps = 0/42 (0%)
 Frame = +1

Query  342      KELYGHLAQELSALAARHGERLLKTPHNPISLAMSLNHLEAH  383
                +E+Y  LAQEL  LA+ HGERLL TPHNPISL   +  L+ H
Sbjct  1599313  QEIYSFLAQELKTLASAHGERLLHTPHNPISLGKLIVLLQNH  1599438


 Score = 50.4 bits (119),  Expect = 4e-05, Method: Compositional matrix adjust.
 Identities = 25/42 (60%), Positives = 30/42 (71%), Gaps = 0/42 (0%)
 Frame = +3

Query  342      KELYGHLAQELSALAARHGERLLKTPHNPISLAMSLNHLEAH  383
                +E+Y  LAQEL  LA+ HGERLL TPHNPISL   +  L+ H
Sbjct  1606299  QEIYSFLAQELKTLASAHGERLLHTPHNPISLGKLIVLLQNH  1606424


 Score = 48.1 bits (113),  Expect = 2e-04, Method: Compositional matrix adjust.
 Identities = 24/31 (77%), Positives = 27/31 (87%), Gaps = 0/31 (0%)
 Frame = +2

Query  374      AMSLNHLEAHSSSAVTQLGSMLFTRQVSGAR  404
                AMSL+ L+A S+ AVT LGSMLFTRQVSGAR
Sbjct  1606547  AMSLDGLQAESNKAVTHLGSMLFTRQVSGAR  1606639



Lambda      K        H        a         alpha
   0.320    0.134    0.389    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 75061097330


  Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
    Posted date:  Oct 21, 2016  4:44 PM
  Number of letters in database: 619,300,777
  Number of sequences in database:  6,294



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40