TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
           6,294 sequences; 619,300,777 total letters



Query= SPP00000627_2.0 # Protein # SECIS binding protein 2 (SBP2) #
Zebrafish

Length=362
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JXSJ01000013.1 Miichthys miiuy scaffold13, whole genome shotgun...    208   6e-59
  JXSJ01000001.1 Miichthys miiuy scaffold1, whole genome shotgun ...    100   2e-21


> JXSJ01000013.1 Miichthys miiuy scaffold13, whole genome shotgun 
sequence
Length=5370402

 Score =   208 bits (530),  Expect(2) = 6e-59, Method: Compositional matrix adjust.
 Identities = 159/352 (45%), Positives = 179/352 (51%), Gaps = 130/352 (37%)
 Frame = -2

Query  42      LTVGGALPVVHKEPSVQKK-QWQQEKTPHNPLDSTCPSVKKGKQREVPKAKKPTPLKRV-  99
                 VGG LPVV K+P VQKK  WQQ K  HNPLDST P VKKGKQREVPKAKKPTPLK+V 
Sbjct  207866  FVVGGGLPVVQKQPLVQKKLHWQQAKIAHNPLDSTSPLVKKGKQREVPKAKKPTPLKKVS  207687

Query  100     ---------------------------------ILREREERKQNRLLEER-----DPSRV  121
                                                IL+EREERKQ RLLEER     +    
Sbjct  207686  PLTYSGFYIDALQFVRMYFSVGTAMMFLSDPQVILKEREERKQRRLLEERGLLPENECET  207507

Query  122     ATEAGEKE-----TNDNAE----------DIGVKDSEIE-------------------QP  147
               A +A E+E     TN   E          D   K S++E                     
Sbjct  207506  ANDAAEEEQEQCDTNATGERKHVKQYNSVDFYCKLSQLEVD*ENHVHSS*YLICVLCQDD  207327

Query  148     ASPSDDKLDDGLSENPT------------------LQTEIPNSNPISKVPKIHSRKFRD-  188
               A    ++LDD    N T                   QT  P + P S+ PKIHSRKFR+ 
Sbjct  207326  AGSLTEELDDQSEINGTNQMVKEVDEETDKDETVEQQTTSPVTCPASR-PKIHSRKFRE*  207150

Query  189     ------------------------------------YCNQVLSKDVDECVSNLLKELVRF  212
                                                   YC+Q+LSKDVDECV++LLKELVRF
Sbjct  207149  DFKRKTRFTVSQLVFLLVVCCFSFSHLSSFEMYVCSYCSQMLSKDVDECVTSLLKELVRF  206970

Query  213     QDRLYQKDPMKARMKRRLVMGLREvlkhlklkkvkcvIISPNCERIQSKGGL  264
               QDRLYQKDPMKARMKRR+VMGLREVLKHLKL+KVKCVIISPNCERIQSKG +
Sbjct  206969  QDRLYQKDPMKARMKRRIVMGLREVLKHLKLRKVKCVIISPNCERIQSKGTV  206814


 Score = 42.4 bits (98),  Expect(2) = 6e-59, Method: Compositional matrix adjust.
 Identities = 29/43 (67%), Positives = 36/43 (84%), Gaps = 0/43 (0%)
 Frame = -1

Query  1       SQQTSQKKSKVPVQLDLGNMLAFLEqkqqsqkskqdqRPVTLT  43
               +++ S KKSKVPVQLD+GNMLA LE+KQQ QK KQD +PV L+
Sbjct  208113  AEKVSGKKSKVPVQLDIGNMLAALEKKQQFQKDKQDTKPVILS  207985


 Score =   107 bits (266),  Expect(2) = 2e-30, Method: Compositional matrix adjust.
 Identities = 48/55 (87%), Positives = 51/55 (93%), Gaps = 0/55 (0%)
 Frame = -1

Query  262     GGLDEALHNIIDTCRDQSVPFVFALSRKALGRCVNKAVPVSLVGIFNYDGAQDHY  316
               GGLDEAL+ IIDTCR+Q VPFVFALSRKALGRCVNKAVPVSLVGIFNYDGAQ  +
Sbjct  206460  GGLDEALYTIIDTCREQGVPFVFALSRKALGRCVNKAVPVSLVGIFNYDGAQVRF  206296


 Score = 48.1 bits (113),  Expect(2) = 2e-30, Method: Compositional matrix adjust.
 Identities = 19/29 (66%), Positives = 27/29 (93%), Gaps = 0/29 (0%)
 Frame = -3

Query  313     QDHYHKMIELSAEARKSYEVMIANLDEEG  341
               QD+YHKMIELS+EAR +YEVM+++L++ G
Sbjct  206224  QDYYHKMIELSSEARTAYEVMVSSLEQTG  206138


> JXSJ01000001.1 Miichthys miiuy scaffold1, whole genome shotgun 
sequence
Length=20211669

 Score =   100 bits (250),  Expect = 2e-21, Method: Compositional matrix adjust.
 Identities = 57/77 (74%), Positives = 69/77 (90%), Gaps = 0/77 (0%)
 Frame = +1

Query  186     FRDYCNQVLSKDVDECVSNLLKELVRFQDRLYQKDPMKARMKRRLVMGLREvlkhlklkk  245
               F  YCNQVLSK++DE V+ LL+ELVRFQ+R+YQKDP KA+ KRRLVMGLREV KH+KL K
Sbjct  189196  FCRYCNQVLSKEIDESVTLLLQELVRFQERVYQKDPTKAKSKRRLVMGLREVTKHMKLHK  189375

Query  246     vkcvIISPNCERIQSKG  262
               +KCV+ISPNCE+IQ+KG
Sbjct  189376  IKCVVISPNCEKIQAKG  189426


 Score = 87.4 bits (215),  Expect = 3e-17, Method: Compositional matrix adjust.
 Identities = 38/53 (72%), Positives = 44/53 (83%), Gaps = 0/53 (0%)
 Frame = +3

Query  261     KGGLDEALHNIIDTCRDQSVPFVFALSRKALGRCVNKAVPVSLVGIFNYDGAQ  313
                GGLDEAL+N+I    +Q +PFVFAL RKALGRCVNK VPVS+VGIFNY GA+
Sbjct  190044  AGGLDEALYNVIAMASEQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAE  190202


 Score = 53.9 bits (128),  Expect = 2e-06, Method: Compositional matrix adjust.
 Identities = 25/45 (56%), Positives = 32/45 (71%), Gaps = 0/45 (0%)
 Frame = +3

Query  55      PSVQKKQWQQEKTPHNPLDSTCPSVKKGKQREVPKAKKPTPLKRV  99
               PS+ K   Q    PHN LDST P +K+GK+RE+PK K+PT LK+V
Sbjct  187590  PSMLKGHQQPYSAPHNSLDSTAPRIKRGKEREIPKVKRPTALKKV  187724



Lambda      K        H        a         alpha
   0.314    0.132    0.377    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 49359773760


  Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
    Posted date:  Oct 21, 2016  4:44 PM
  Number of letters in database: 619,300,777
  Number of sequences in database:  6,294



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40