TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
           6,294 sequences; 619,300,777 total letters



Query= SPP00000644_2.0 # Protein # Methionine-R-sufoxide reductase 1b
(MSRB1b) # Zebrafish

Length=104
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JXSJ01000015.1 Miichthys miiuy scaffold15, whole genome shotgun...    147   7e-40
  JXSJ01000046.1 Miichthys miiuy scaffold46, whole genome shotgun...  90.9    1e-21


> JXSJ01000015.1 Miichthys miiuy scaffold15, whole genome shotgun 
sequence
Length=4954414

 Score =   147 bits (371),  Expect = 7e-40, Method: Compositional matrix adjust.
 Identities = 76/126 (60%), Positives = 81/126 (64%), Gaps = 41/126 (33%)
 Frame = -1

Query  19       GIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMETLTAYK------------  66
                GIY CSQC HPLFSSRSK+ HSSPWPAFT+TIREDSVTKMMETLTA+K            
Sbjct  2036248  GIYVCSQCNHPLFSSRSKFAHSSPWPAFTDTIREDSVTKMMETLTAFKVRNHNLSSYMFY  2036069

Query  67       -----------------------------VLCGKCGNGLGHEFLNDGPEEGLSRFXIFSH  97
                                             VLCGKCGNGLGHEF+NDGPEEG+SRF IFSH
Sbjct  2036068  YWFLFVLL*NICSSGYGT*AGCDCVSCIQVLCGKCGNGLGHEFVNDGPEEGVSRF*IFSH  2035889

Query  98       SLKFVP  103
                SLKFVP
Sbjct  2035888  SLKFVP  2035871


 Score = 37.7 bits (86),  Expect = 0.012, Method: Compositional matrix adjust.
 Identities = 17/24 (71%), Positives = 18/24 (75%), Gaps = 0/24 (0%)
 Frame = -1

Query  1        MSFCSFFGKEFYKDHFKPGIYACS  24
                MSFC FFG E YKDHFKPG  A +
Sbjct  2037319  MSFCQFFGGEVYKDHFKPGEQAVA  2037248


> JXSJ01000046.1 Miichthys miiuy scaffold46, whole genome shotgun 
sequence
Length=2451071

 Score = 90.9 bits (224),  Expect(2) = 1e-21, Method: Compositional matrix adjust.
 Identities = 59/133 (44%), Positives = 65/133 (49%), Gaps = 47/133 (35%)
 Frame = -1

Query  19       GIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMETLTAYKV-LCGKCGNGL-  76
                G+Y CS+CGH LFSS SK+EHSSPWPAF+ETI EDSV+K  E     KV L   C N L 
Sbjct  2416109  GVYVCSECGHELFSSTSKFEHSSPWPAFSETIHEDSVSKHPEAWGPLKVDLLDICHNKLH  2415930

Query  77       ---------------------------------------------GHEFLNDGPEEGLSR  91
                                                             GHEFL DGP EGLSR
Sbjct  2415929  HIKLNLMSTVTLVINHAV*I*DFNLNLLDLCVLK*VCCGKCGGGLGHEFLYDGPREGLSR  2415750

Query  92       FXIFSHSLKFVPK  104
                F IFS SL F+PK
Sbjct  2415749  F*IFSSSLTFIPK  2415711


 Score = 31.6 bits (70),  Expect(2) = 1e-21, Method: Compositional matrix adjust.
 Identities = 13/19 (68%), Positives = 16/19 (84%), Gaps = 0/19 (0%)
 Frame = -3

Query  1        MSFCSFFGKEFYKDHFKPG  19
                MS+CSF G E YK+HF+PG
Sbjct  2416260  MSYCSFSGGEEYKNHFQPG  2416204


 Score = 74.3 bits (181),  Expect = 1e-14, Method: Compositional matrix adjust.
 Identities = 36/49 (73%), Positives = 39/49 (80%), Gaps = 0/49 (0%)
 Frame = -3

Query  19       GIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMETLTAYKV  67
                GIY C++C H LFSS SKYEHSSPWPAFTETI EDSV+K  E   AYKV
Sbjct  2418156  GIYVCAKCEHQLFSSHSKYEHSSPWPAFTETILEDSVSKHEERPGAYKV  2418010


 Score = 68.2 bits (165),  Expect = 1e-12, Method: Compositional matrix adjust.
 Identities = 31/39 (79%), Positives = 35/39 (90%), Gaps = 0/39 (0%)
 Frame = -2

Query  66       KVLCGKCGNGLGHEFLNDGPEEGLSRFXIFSHSLKFVPK  104
                +V CGKCGNGLGHEF+NDGP +GLSRF IFS SLKF+PK
Sbjct  2417884  QVRCGKCGNGLGHEFVNDGPAKGLSRF*IFSSSLKFIPK  2417768



Lambda      K        H        a         alpha
   0.323    0.138    0.452    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 5148409150


  Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
    Posted date:  Oct 21, 2016  4:44 PM
  Number of letters in database: 619,300,777
  Number of sequences in database:  6,294



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40