TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
           6,294 sequences; 619,300,777 total letters



Query= SPP00000645_2.0 # Protein # Methionine-R-sufoxide reductase 1a
(MSRB1a) # Zebrafish

Length=110
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JXSJ01000015.1 Miichthys miiuy scaffold15, whole genome shotgun...    124   8e-32
  JXSJ01000046.1 Miichthys miiuy scaffold46, whole genome shotgun...    104   4e-26


> JXSJ01000015.1 Miichthys miiuy scaffold15, whole genome shotgun 
sequence
Length=4954414

 Score =   124 bits (311),  Expect = 8e-32, Method: Compositional matrix adjust.
 Identities = 68/144 (47%), Positives = 80/144 (56%), Gaps = 49/144 (34%)
 Frame = -1

Query  1        MSFCSFSGGEIYKDHFESGMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSKQEE  60
                + FC+FS          SG+YVC+QC + LFSSRSK+ HSSPWPAFT+TI EDSV+K  E
Sbjct  2036278  LDFCAFSP--------PSGIYVCSQCNHPLFSSRSKFAHSSPWPAFTDTIREDSVTKMME  2036123

Query  61       RWGAYKVR-----------------------------------------CGKCGNGLGHE  79
                   A+KVR                                         CGKCGNGLGHE
Sbjct  2036122  TLTAFKVRNHNLSSYMFYYWFLFVLL*NICSSGYGT*AGCDCVSCIQVLCGKCGNGLGHE  2035943

Query  80       FVNDGPKHGLSRFXIFSSSLKFIP  103
                FVNDGP+ G+SRF IFS SLKF+P
Sbjct  2035942  FVNDGPEEGVSRF*IFSHSLKFVP  2035871


> JXSJ01000046.1 Miichthys miiuy scaffold46, whole genome shotgun 
sequence
Length=2451071

 Score =   104 bits (259),  Expect(2) = 4e-26, Method: Compositional matrix adjust.
 Identities = 63/134 (47%), Positives = 71/134 (53%), Gaps = 47/134 (35%)
 Frame = -1

Query  18       SGMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSKQEERWGAYKVR-CGKCGNGL  76
                SG+YVC++CG+ELFSS SK+EHSSPWPAF+ETIHEDSVSK  E WG  KV     C N L
Sbjct  2416112  SGVYVCSECGHELFSSTSKFEHSSPWPAFSETIHEDSVSKHPEAWGPLKVDLLDICHNKL  2415933

Query  77       ----------------------------------------------GHEFVNDGPKHGLS  90
                                                              GHEF+ DGP+ GLS
Sbjct  2415932  HHIKLNLMSTVTLVINHAV*I*DFNLNLLDLCVLK*VCCGKCGGGLGHEFLYDGPREGLS  2415753

Query  91       RFXIFSSSLKFIPK  104
                RF IFSSSL FIPK
Sbjct  2415752  RF*IFSSSLTFIPK  2415711


 Score = 33.5 bits (75),  Expect(2) = 4e-26, Method: Compositional matrix adjust.
 Identities = 14/19 (74%), Positives = 17/19 (89%), Gaps = 0/19 (0%)
 Frame = -3

Query  1        MSFCSFSGGEIYKDHFESG  19
                MS+CSFSGGE YK+HF+ G
Sbjct  2416260  MSYCSFSGGEEYKNHFQPG  2416204


 Score = 84.3 bits (207),  Expect = 5e-18, Method: Compositional matrix adjust.
 Identities = 40/49 (82%), Positives = 44/49 (90%), Gaps = 0/49 (0%)
 Frame = -3

Query  19       GMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSKQEERWGAYKV  67
                G+YVCA+C ++LFSS SKYEHSSPWPAFTETI EDSVSK EER GAYKV
Sbjct  2418156  GIYVCAKCEHQLFSSHSKYEHSSPWPAFTETILEDSVSKHEERPGAYKV  2418010


 Score = 73.9 bits (180),  Expect = 2e-14, Method: Compositional matrix adjust.
 Identities = 35/39 (90%), Positives = 36/39 (92%), Gaps = 0/39 (0%)
 Frame = -2

Query  66       KVRCGKCGNGLGHEFVNDGPKHGLSRFXIFSSSLKFIPK  104
                +VRCGKCGNGLGHEFVNDGP  GLSRF IFSSSLKFIPK
Sbjct  2417884  QVRCGKCGNGLGHEFVNDGPAKGLSRF*IFSSSLKFIPK  2417768


 Score = 37.0 bits (84),  Expect = 0.021, Method: Compositional matrix adjust.
 Identities = 15/22 (68%), Positives = 19/22 (86%), Gaps = 0/22 (0%)
 Frame = -2

Query  1        MSFCSFSGGEIYKDHFESGMYV  22
                MSFCSF GGE +KDHF+SG ++
Sbjct  2418712  MSFCSFFGGEAFKDHFKSGTFM  2418647



Lambda      K        H        a         alpha
   0.319    0.135    0.435    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 5147465050


  Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
    Posted date:  Oct 21, 2016  4:44 PM
  Number of letters in database: 619,300,777
  Number of sequences in database:  6,294



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40