TBLASTN 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa 6,294 sequences; 619,300,777 total letters Query= SPP00000622_2.0 # Protein # Glutathione peroxidase 4b (GPx4b) # Zebrafish Length=130 Score E Sequences producing significant alignments: (Bits) Value JXSJ01002762.1 Miichthys miiuy scaffold2762, whole genome shotg... 110 6e-27 JXSJ01000006.1 Miichthys miiuy scaffold6, whole genome shotgun ... 101 2e-23 JXSJ01000014.1 Miichthys miiuy scaffold14, whole genome shotgun... 81.6 9e-17 JXSJ01000525.1 Miichthys miiuy scaffold525, whole genome shotgu... 71.6 2e-13 JXSJ01000013.1 Miichthys miiuy scaffold13, whole genome shotgun... 63.5 9e-11 JXSJ01000043.1 Miichthys miiuy scaffold43, whole genome shotgun... 59.7 2e-09 JXSJ01000122.1 Miichthys miiuy scaffold122, whole genome shotgu... 58.9 3e-09 JXSJ01000061.1 Miichthys miiuy scaffold61, whole genome shotgun... 49.7 3e-06 JXSJ01000139.1 Miichthys miiuy scaffold139, whole genome shotgu... 35.4 3e-05 JXSJ01000010.1 Miichthys miiuy scaffold10, whole genome shotgun... 43.9 2e-04 JXSJ01005752.1 Miichthys miiuy scaffold5752, whole genome shotg... 43.1 2e-04 > JXSJ01002762.1 Miichthys miiuy scaffold2762, whole genome shotgun sequence Length=6586 Score = 110 bits (276), Expect = 6e-27, Method: Composition-based stats. Identities = 65/150 (43%), Positives = 85/150 (57%), Gaps = 21/150 (14%) Frame = -1 Query 1 FRGYVCIITNVASKXGKTPVNYTQLAAMHVTYAEKGLRILGFPCNQFGKQEPGSEAEIKE 60 F+G V ++ NVASK G TP Y L ++ + E GL +LGFP N F +QEPGS+ EIK Sbjct 4444 FQGKVLLVVNVASKCGLTP-QYAGLEKLYEEHREAGLEVLGFPANDFREQEPGSDEEIKA 4268 Query 61 FAK-GYNAEFDLFSKIDVNGDAAHPLWKWMKE-QPK--GRGTLGNNIK------------ 104 F Y +F LFSKI V G HPL++ + E QP+ G G + +K Sbjct 4267 FCSLNYAVQFPLFSKIAVTGADRHPLYRLLTEAQPQTTGEGPMRERLKGYGIEPGPLPEV 4088 Query 105 -WNFTKFLIDREGQVVKRYGP---MDDPSV 130 WNF KFL+ R+GQVV R+ P DDP + Sbjct 4087 LWNFEKFLVSRDGQVVARFAPDVAADDPRL 3998 > JXSJ01000006.1 Miichthys miiuy scaffold6, whole genome shotgun sequence Length=7996890 Score = 101 bits (251), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 49/70 (70%), Positives = 56/70 (80%), Gaps = 0/70 (0%) Frame = -1 Query 50 QEPGSEAEIKEFAKGYNAEFDLFSKIDVNGDAAHPLWKWMKEQPKGRGTLGNNIKWNFTK 109 QEPG++AEIKEFAKGYNAEFDLFSKIDVNGD AHPLWKWMK QPKG+GTLG + F+ Sbjct 3978360 QEPGTDAEIKEFAKGYNAEFDLFSKIDVNGDNAHPLWKWMKAQPKGKGTLGK*VICLFSC 3978181 Query 110 FLIDREGQVV 119 L + +V Sbjct 3978180 LLNIKSHDIV 3978151 Score = 88.2 bits (217), Expect = 5e-19, Method: Composition-based stats. Identities = 41/49 (84%), Positives = 42/49 (86%), Gaps = 0/49 (0%) Frame = -3 Query 2 RGYVCIITNVASKXGKTPVNYTQLAAMHVTYAEKGLRILGFPCNQFGKQ 50 RG VCII NVASK GKT VNYTQLA MH +YAEKGLRILGFPCNQFG Q Sbjct 3978784 RGDVCIIVNVASK*GKTRVNYTQLAGMHASYAEKGLRILGFPCNQFGGQ 3978638 Score = 43.9 bits (102), Expect = 2e-04, Method: Composition-based stats. Identities = 18/22 (82%), Positives = 20/22 (91%), Gaps = 0/22 (0%) Frame = -2 Query 109 KFLIDREGQVVKRYGPMDDPSV 130 +FLI+REG VVKRYGP DDPSV Sbjct 3977243 QFLINREGLVVKRYGPTDDPSV 3977178 > JXSJ01000014.1 Miichthys miiuy scaffold14, whole genome shotgun sequence Length=5141783 Score = 81.6 bits (200), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 34/54 (63%), Positives = 45/54 (83%), Gaps = 0/54 (0%) Frame = -3 Query 47 FGKQEPGSEAEIKEFAKGYNAEFDLFSKIDVNGDAAHPLWKWMKEQPKGRGTLG 100 F +EPG+E +IK+FA+ Y+ +FD+FSKI+VNGD AHPLWKW+K QP G+G LG Sbjct 7932 FTLKEPGNETQIKQFAESYHVQFDMFSKINVNGDNAHPLWKWLKNQPNGKGFLG 7771 Score = 77.8 bits (190), Expect = 2e-15, Method: Composition-based stats. Identities = 36/50 (72%), Positives = 39/50 (78%), Gaps = 0/50 (0%) Frame = -1 Query 1 FRGYVCIITNVASKXGKTPVNYTQLAAMHVTYAEKGLRILGFPCNQFGKQ 50 FRG V IITNVASK GKT VNY+Q A +H YAE+GLRIL FP NQFG Q Sbjct 8327 FRGSVVIITNVASK*GKTLVNYSQFADLHARYAERGLRILAFPSNQFGNQ 8178 Score = 47.4 bits (111), Expect = 1e-05, Method: Composition-based stats. Identities = 19/21 (90%), Positives = 21/21 (100%), Gaps = 0/21 (0%) Frame = -1 Query 110 FLIDREGQVVKRYGPMDDPSV 130 FLI+REGQVVKRYGP+DDPSV Sbjct 7205 FLINREGQVVKRYGPLDDPSV 7143 > JXSJ01000525.1 Miichthys miiuy scaffold525, whole genome shotgun sequence Length=204830 Score = 71.6 bits (174), Expect = 2e-13, Method: Composition-based stats. Identities = 35/86 (41%), Positives = 52/86 (60%), Gaps = 1/86 (1%) Frame = +2 Query 5 VCIITNVASKXGKTPVNYTQLAAMHVTYAEKGLRILGFPCNQFGKQEPGSEAEIKEFAKG 64 V ++ NVAS+ G T +Y ++ + + +L FPCNQFG+QEPGS+ EI F + Sbjct 179948 VSLVVNVASECGFTEEHYKEMQQLQRDFGPYHFNVLAFPCNQFGQQEPGSDKEIDSFVRR 180127 Query 65 -YNAEFDLFSKIDVNGDAAHPLWKWM 89 Y F LFSKI V G A+ ++K++ Sbjct 180128 VYGVSFPLFSKIAVVGTGANNVYKYL 180205 > JXSJ01000013.1 Miichthys miiuy scaffold13, whole genome shotgun sequence Length=5370402 Score = 63.5 bits (153), Expect = 9e-11, Method: Composition-based stats. Identities = 29/84 (35%), Positives = 44/84 (52%), Gaps = 1/84 (1%) Frame = -1 Query 7 IITNVASKXGKTPVNYTQLAAMHVTYAEKGLRILGFPCNQFGKQEPGSEAEIKEFAKG-Y 65 ++ NVAS +T NY + +H +L FPC QFG E G+ +I+ FAK Y Sbjct 1302507 LVVNVASHCEQTEANYRSMQELHRELGTSHFNVLAFPCGQFGDTETGTSRDIEAFAKSTY 1302328 Query 66 NAEFDLFSKIDVNGDAAHPLWKWM 89 F FS+I + G A P ++++ Sbjct 1302327 GVTFPFFSRIKIMGSEADPAFRFL 1302256 > JXSJ01000043.1 Miichthys miiuy scaffold43, whole genome shotgun sequence Length=2573463 Score = 59.7 bits (143), Expect = 2e-09, Method: Composition-based stats. Identities = 28/50 (56%), Positives = 33/50 (66%), Gaps = 0/50 (0%) Frame = -3 Query 1 FRGYVCIITNVASKXGKTPVNYTQLAAMHVTYAEKGLRILGFPCNQFGKQ 50 +G V +I NVAS G T +YTQ+ +H YA KGL ILG PCNQFG Q Sbjct 1231819 LKGKVVLIENVASL*GTTTRDYTQMNELHSRYAAKGLVILGVPCNQFGHQ 1231670 Score = 43.5 bits (101), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 23/77 (30%), Positives = 38/77 (49%), Gaps = 17/77 (22%) Frame = -2 Query 64 GYNAEFDLFSKIDVNGDAAHPLWKWMKEQPKGRG-----------------TLGNNIKWN 106 G+ +F + K++VNG AHPL+ ++K++ + N+I WN Sbjct 1231166 GFEPKFKILEKVNVNGKDAHPLFVFLKDKLRFPSDDAMALMNDPQCIIWSPVCRNDISWN 1230987 Query 107 FTKFLIDREGQVVKRYG 123 F KFL+ +G+ KRY Sbjct 1230986 FEKFLVGPDGEPYKRYS 1230936 > JXSJ01000122.1 Miichthys miiuy scaffold122, whole genome shotgun sequence Length=783778 Score = 58.9 bits (141), Expect = 3e-09, Method: Composition-based stats. Identities = 28/50 (56%), Positives = 33/50 (66%), Gaps = 0/50 (0%) Frame = -2 Query 1 FRGYVCIITNVASKXGKTPVNYTQLAAMHVTYAEKGLRILGFPCNQFGKQ 50 +G V +I NVAS G T +YTQ+ +H YA KGL ILG PCNQFG Q Sbjct 470517 LQGKVVLIENVASL*GTTTRDYTQMNELHDRYASKGLVILGVPCNQFGHQ 470368 Score = 42.4 bits (98), Expect = 5e-04, Method: Composition-based stats. Identities = 26/76 (34%), Positives = 36/76 (47%), Gaps = 17/76 (22%) Frame = -2 Query 64 GYNAEFDLFSKIDVNGDAAHPLWKWMKEQ--------------PK---GRGTLGNNIKWN 106 G+ +F L K+DVNG AH L+ +++E PK N++ WN Sbjct 469281 GFEPKFQLLEKVDVNGKDAHSLFVFLRESLPSPSDEPCAFITDPKLVIWSPVCRNDVAWN 469102 Query 107 FTKFLIDREGQVVKRY 122 F KFLI +G KRY Sbjct 469101 FEKFLIGPDGVPFKRY 469054 > JXSJ01000061.1 Miichthys miiuy scaffold61, whole genome shotgun sequence Length=1726861 Score = 49.7 bits (117), Expect = 3e-06, Method: Composition-based stats. Identities = 24/50 (48%), Positives = 32/50 (64%), Gaps = 1/50 (2%) Frame = +3 Query 1 FRGYVCIITNVASKXGKTPVNYTQLAAMHVTYAEKGLRILGFPCNQFGKQ 50 FRG V +I NVAS G T ++++L + Y + L +LGFPCNQFG Q Sbjct 671031 FRGRVVLIENVASL*GTTTRDFSELNQLQSKYPHR-LVVLGFPCNQFGYQ 671177 Score = 42.0 bits (97), Expect = 7e-04, Method: Compositional matrix adjust. Identities = 23/81 (28%), Positives = 36/81 (44%), Gaps = 25/81 (31%) Frame = +3 Query 64 GYNAEFDLFSKIDVNGDAAHPLWKWMKEQ---------------------PKGRGTLGNN 102 G+ F +F K DVNG HP++ ++K++ P R + Sbjct 671754 GFQPNFTIFEKCDVNGTNTHPVFAYLKDKLPYPDDDPSSLMQDPKFLIWSPINR----TD 671921 Query 103 IKWNFTKFLIDREGQVVKRYG 123 + WNF KF++ EG+ KRY Sbjct 671922 VSWNFEKFIVGPEGEPFKRYS 671984 > JXSJ01000139.1 Miichthys miiuy scaffold139, whole genome shotgun sequence Length=664307 Score = 35.4 bits (80), Expect(2) = 3e-05, Method: Composition-based stats. Identities = 16/28 (57%), Positives = 21/28 (75%), Gaps = 0/28 (0%) Frame = -2 Query 96 RGTLGNNIKWNFTKFLIDREGQVVKRYG 123 +G LG +WNFTKFL+ REG VV+ +G Sbjct 648193 QGNLGE*HQWNFTKFLMHREGVVVEAHG 648110 Score = 31.2 bits (69), Expect(2) = 3e-05, Method: Composition-based stats. Identities = 10/21 (48%), Positives = 14/21 (67%), Gaps = 0/21 (0%) Frame = -1 Query 80 DAAHPLWKWMKEQPKGRGTLG 100 D AHP W+W+++ PK R G Sbjct 648239 DNAHPPWRWIRQSPKAREPWG 648177 > JXSJ01000010.1 Miichthys miiuy scaffold10, whole genome shotgun sequence Length=6703581 Score = 43.9 bits (102), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 19/23 (83%), Positives = 20/23 (87%), Gaps = 0/23 (0%) Frame = +3 Query 36 GLRILGFPCNQFGKQEPGSEAEI 58 GL ILGFPCNQFGKQEPG + EI Sbjct 2819757 GLTILGFPCNQFGKQEPGQKHEI 2819825 > JXSJ01005752.1 Miichthys miiuy scaffold5752, whole genome shotgun sequence Length=1069 Score = 43.1 bits (100), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 26/77 (34%), Positives = 36/77 (47%), Gaps = 17/77 (22%) Frame = +1 Query 64 GYNAEFDLFSKIDVNGDAAHPLWKWMKEQ--------------PK---GRGTLGNNIKWN 106 G+ +F L K+DVNG AH L+ +++E PK N++ WN Sbjct 163 GFEPKFQLLEKVDVNGKDAHSLFVFLRESLPSPSDEPCAFITDPKLVIWSPVCRNDVAWN 342 Query 107 FTKFLIDREGQVVKRYG 123 F KFLI +G KRY Sbjct 343 FEKFLIGPDGVPFKRYS 393 Lambda K H a alpha 0.319 0.138 0.434 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 5144318050 Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa Posted date: Oct 21, 2016 4:44 PM Number of letters in database: 619,300,777 Number of sequences in database: 6,294 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40