TBLASTN 2.2.30+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
           6,294 sequences; 619,300,777 total letters



Query= SPP00000618_2.0 # Protein # Glutathione peroxidase 4a (GPx4a) #
Zebrafish

Length=113
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  JXSJ01000006.1 Miichthys miiuy scaffold6, whole genome shotgun ...  92.0    1e-20
  JXSJ01000014.1 Miichthys miiuy scaffold14, whole genome shotgun...  90.9    3e-20
  JXSJ01002762.1 Miichthys miiuy scaffold2762, whole genome shotg...  87.4    4e-19
  JXSJ01000525.1 Miichthys miiuy scaffold525, whole genome shotgu...  68.9    9e-13
  JXSJ01000013.1 Miichthys miiuy scaffold13, whole genome shotgun...  64.3    3e-11
  JXSJ01000043.1 Miichthys miiuy scaffold43, whole genome shotgun...  60.5    6e-10
  JXSJ01000122.1 Miichthys miiuy scaffold122, whole genome shotgu...  58.5    2e-09
  JXSJ01000061.1 Miichthys miiuy scaffold61, whole genome shotgun...  53.1    2e-07
  JXSJ01000010.1 Miichthys miiuy scaffold10, whole genome shotgun...  36.6    0.032


> JXSJ01000006.1 Miichthys miiuy scaffold6, whole genome shotgun 
sequence
Length=7996890

 Score = 92.0 bits (227),  Expect = 1e-20, Method: Compositional matrix adjust.
 Identities = 41/51 (80%), Positives = 46/51 (90%), Gaps = 0/51 (0%)
 Frame = -1

Query  53       QEPGTNSQIKEFAKSYNAEFDMFSKIDVNGDGAHPLWKWLKDQPNGKGFLG  103
                QEPGT+++IKEFAK YNAEFD+FSKIDVNGD AHPLWKW+K QP GKG LG
Sbjct  3978360  QEPGTDAEIKEFAKGYNAEFDLFSKIDVNGDNAHPLWKWMKAQPKGKGTLG  3978208


 Score = 73.9 bits (180),  Expect = 2e-14, Method: Composition-based stats.
 Identities = 36/53 (68%), Positives = 39/53 (74%), Gaps = 0/53 (0%)
 Frame = -3

Query  1        FTYFRGKVVIITNVASKXGKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQ  53
                F   RG V II NVASK GKT VNY+Q A MHA Y+E+GLRIL FP NQFG Q
Sbjct  3978796  FFSARGDVCIIVNVASK*GKTRVNYTQLAGMHASYAEKGLRILGFPCNQFGGQ  3978638


> JXSJ01000014.1 Miichthys miiuy scaffold14, whole genome shotgun 
sequence
Length=5141783

 Score = 90.9 bits (224),  Expect = 3e-20, Method: Compositional matrix adjust.
 Identities = 40/54 (74%), Positives = 48/54 (89%), Gaps = 0/54 (0%)
 Frame = -3

Query  50    FGRQEPGTNSQIKEFAKSYNAEFDMFSKIDVNGDGAHPLWKWLKDQPNGKGFLG  103
             F  +EPG  +QIK+FA+SY+ +FDMFSKI+VNGD AHPLWKWLK+QPNGKGFLG
Sbjct  7932  FTLKEPGNETQIKQFAESYHVQFDMFSKINVNGDNAHPLWKWLKNQPNGKGFLG  7771


 Score = 89.0 bits (219),  Expect = 2e-19, Method: Composition-based stats.
 Identities = 42/51 (82%), Positives = 47/51 (92%), Gaps = 0/51 (0%)
 Frame = -1

Query  3     YFRGKVVIITNVASKXGKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQ  53
             +FRG VVIITNVASK GKT VNYSQFA++HA+Y+ERGLRILAFPSNQFG Q
Sbjct  8330  FFRGSVVIITNVASK*GKTLVNYSQFADLHARYAERGLRILAFPSNQFGNQ  8178


> JXSJ01002762.1 Miichthys miiuy scaffold2762, whole genome shotgun 
sequence
Length=6586

 Score = 87.4 bits (215),  Expect = 4e-19, Method: Composition-based stats.
 Identities = 50/126 (40%), Positives = 74/126 (59%), Gaps = 18/126 (14%)
 Frame = -1

Query  4     FRGKVVIITNVASKXGKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQEPGTNSQIKE  63
             F+GKV+++ NVASK G TP  Y+   +++ ++ E GL +L FP+N F  QEPG++ +IK 
Sbjct  4444  FQGKVLLVVNVASKCGLTP-QYAGLEKLYEEHREAGLEVLGFPANDFREQEPGSDEEIKA  4268

Query  64    FAK-SYNAEFDMFSKIDVNGDGAHPLWKWLKD---QPNGKGFL-----GNGIK-------  107
             F   +Y  +F +FSKI V G   HPL++ L +   Q  G+G +     G GI+       
Sbjct  4267  FCSLNYAVQFPLFSKIAVTGADRHPLYRLLTEAQPQTTGEGPMRERLKGYGIEPGPLPEV  4088

Query  108   -WNFTK  112
              WNF K
Sbjct  4087  LWNFEK  4070


> JXSJ01000525.1 Miichthys miiuy scaffold525, whole genome shotgun 
sequence
Length=204830

 Score = 68.9 bits (167),  Expect = 9e-13, Method: Composition-based stats.
 Identities = 34/86 (40%), Positives = 53/86 (62%), Gaps = 1/86 (1%)
 Frame = +2

Query  8       VVIITNVASKXGKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQEPGTNSQIKEFAKS  67
               V ++ NVAS+ G T  +Y +  ++   +      +LAFP NQFG+QEPG++ +I  F + 
Sbjct  179948  VSLVVNVASECGFTEEHYKEMQQLQRDFGPYHFNVLAFPCNQFGQQEPGSDKEIDSFVRR  180127

Query  68      -YNAEFDMFSKIDVNGDGAHPLWKWL  92
                Y   F +FSKI V G GA+ ++K+L
Sbjct  180128  VYGVSFPLFSKIAVVGTGANNVYKYL  180205


> JXSJ01000013.1 Miichthys miiuy scaffold13, whole genome shotgun 
sequence
Length=5370402

 Score = 64.3 bits (155),  Expect = 3e-11, Method: Composition-based stats.
 Identities = 33/84 (39%), Positives = 46/84 (55%), Gaps = 1/84 (1%)
 Frame = -1

Query  10       IITNVASKXGKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQEPGTNSQIKEFAKS-Y  68
                ++ NVAS   +T  NY    E+H +       +LAFP  QFG  E GT+  I+ FAKS Y
Sbjct  1302507  LVVNVASHCEQTEANYRSMQELHRELGTSHFNVLAFPCGQFGDTETGTSRDIEAFAKSTY  1302328

Query  69       NAEFDMFSKIDVNGDGAHPLWKWL  92
                   F  FS+I + G  A P +++L
Sbjct  1302327  GVTFPFFSRIKIMGSEADPAFRFL  1302256


> JXSJ01000043.1 Miichthys miiuy scaffold43, whole genome shotgun 
sequence
Length=2573463

 Score = 60.5 bits (145),  Expect = 6e-10, Method: Composition-based stats.
 Identities = 27/53 (51%), Positives = 37/53 (70%), Gaps = 0/53 (0%)
 Frame = -3

Query  1        FTYFRGKVVIITNVASKXGKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQ  53
                F+  +GKVV+I NVAS  G T  +Y+Q  E+H++Y+ +GL IL  P NQFG Q
Sbjct  1231828  FSALKGKVVLIENVASL*GTTTRDYTQMNELHSRYAAKGLVILGVPCNQFGHQ  1231670


> JXSJ01000122.1 Miichthys miiuy scaffold122, whole genome shotgun 
sequence
Length=783778

 Score = 58.5 bits (140),  Expect = 2e-09, Method: Composition-based stats.
 Identities = 27/53 (51%), Positives = 36/53 (68%), Gaps = 0/53 (0%)
 Frame = -2

Query  1       FTYFRGKVVIITNVASKXGKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQ  53
               F+  +GKVV+I NVAS  G T  +Y+Q  E+H +Y+ +GL IL  P NQFG Q
Sbjct  470526  FSSLQGKVVLIENVASL*GTTTRDYTQMNELHDRYASKGLVILGVPCNQFGHQ  470368


> JXSJ01000061.1 Miichthys miiuy scaffold61, whole genome shotgun 
sequence
Length=1726861

 Score = 53.1 bits (126),  Expect = 2e-07, Method: Composition-based stats.
 Identities = 26/53 (49%), Positives = 35/53 (66%), Gaps = 1/53 (2%)
 Frame = +3

Query  1       FTYFRGKVVIITNVASKXGKTPVNYSQFAEMHAKYSERGLRILAFPSNQFGRQ  53
               F  FRG+VV+I NVAS  G T  ++S+  ++ +KY  R L +L FP NQFG Q
Sbjct  671022  FNVFRGRVVLIENVASL*GTTTRDFSELNQLQSKYPHR-LVVLGFPCNQFGYQ  671177


> JXSJ01000010.1 Miichthys miiuy scaffold10, whole genome shotgun 
sequence
Length=6703581

 Score = 36.6 bits (83),  Expect = 0.032, Method: Composition-based stats.
 Identities = 15/23 (65%), Positives = 17/23 (74%), Gaps = 0/23 (0%)
 Frame = +3

Query  39       GLRILAFPSNQFGRQEPGTNSQI  61
                GL IL FP NQFG+QEPG   +I
Sbjct  2819757  GLTILGFPCNQFGKQEPGQKHEI  2819825



Lambda      K        H        a         alpha
   0.318    0.136    0.423    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 5146993000


  Database: /export/cursos/20428/BI/genomes/2016/Miichthys_miiuy/genome.fa
    Posted date:  Oct 21, 2016  4:44 PM
  Number of letters in database: 619,300,777
  Number of sequences in database:  6,294



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40