Total number of SECIS elements predicted: 3
Number of known selenoproteins predicted: 1
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Selenoprotein id: 1         Category: known selenoprotein
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Protein prediction
Predicted by: exonerate Blastx evalue: 2e-26
Query protein: gi|884966827|ref|XP_010877918.2| PREDICTED: selenoprotein N [Esox lucius]
Positions on query: 74-568 Query length: 568

Target name: JARO01007492.1:subseq_15446,85386_
Positions on target: 50249-50369,50045-50175,49452-49664,49152-49276,48621-48758,48369-48450,48092-48286,47295-47400,46971-47083,45956-46057,45632-45799 Strand: -


  Query   HEVAIKALGTEGLFVFSSLDTDHDLYLSPEEFKPIVEKLTG <---Intron---> ITPPADFEEEITH
           |  //||| ||||/||||||| |||||||||||| ||| | <    73bp    > //|||/||||   
  Target  QEAGLRALGAEGLFLFSSLDTDRDLYLSPEEFKPIAEKLAG                LSPPAEFEEEEDR
          cgggccgcggggctctttcgagcgctcacggtacaggacgg                ctccggtgggggc
          aacgtgctgcagttttcctacagatatgcaatactcaatc                gtccccataaaaag
          gactggcgtcgcgcccccgcgcccgcccgggcacccggca                ggtagcgcaaggta
                                                                                
  
  Query   DPNGETLTLEAKMKPLNLDTMTKSKDGFLGV <---Intron---> THSSLSGLRAWKSPSIPSMSFSA
          ||||||||/||/|/|| /||||||||||||| <   380bp    > |||||||||/||||//||   ||
  Target  DPNGETLTVEARMQPLRMDTMTKSKDGFLGV                THSSLSGLRSWKSPAVPSTVISA
          gcaggacagggaacccaagaaaaaaggtcgg                acatcagccttaacggctagatg
          acagactctacgtactgtactcagaagttgt                cagctggtgcgagcctcccttcc
          ccccgcgggggaggccggccgcgcgccccg                tcctcgtggtcggtgtggcgcccc
                                                                                
  
  Query   SQFRAFLPPKNKGTVGDTWWVIPSELNIFSGYLPNNRYHPPVPRGKEV <---Intron---> LINSLL
           |||||||||/|  ||| ||//|||||||/|||||||| || |||||| <   175bp    > /|/|||
  Target  RQFRAFLPPKDKLDVGDAWWIVPSELNIFTGYLPNNRYQPPPPRGKEV                IIHSLL
          cctagtcccagacgggggttagcagcaatagtccaactccccccgagg                aactcc
          gatgcttccaaatatgacggttcgatattcgatcaagaaccccggaat                ttactt
          cgcgccggcgcggccgctggtcctgcccccgcgcccgcgacagggagg                ccccgc
                                                                                
  
  Query   SMFHPRPFIKPRFAPQGTVACVRALSDFYVDIVFRI <---Intron---> HAEFQLNEVPDFPFWFTP
          |||||||||| |||||| | |/|| |//| || ||| <   393bp    > |||||||/||||||||||
  Target  SMFHPRPFIKTRFAPQGAVGCIRAASEYYFDIAFRI                HAEFQLNDVPDFPFWFTP
          aatcccctaaactgccggggtacggagtttgagtaa                cggtccaggcgtctttac
          gttacgcttacgtccagctggtgccgaaatatctgt                acatataatcatctgtcc
          cgtcggtccattcccggtggccccccgtccccccg                ccggtgccctctcccgctg
                                                                                
  
  Query   GQFTGNIIMSRDSSHIRHFLLYVPNDRT <---Intron---> LNVDMEWLYGASESSNMEVDIGYLPQ
          |||||////|||/||/| | |||||||/ <   170bp    > ||||||||||||||||||||||||||
  Target  GQFTGHVLLSRDASHVREFHLYVPNDRS                LNVDMEWLYGASESSNMEVDIGYLPQ
          gctagcgcctcggtcgcgtcctgcagat                caggagtctggagaaaagggagtccc
          gatcgatttcgaccatgatatatcaagc                tatatagtagcgaggatatatgatca
          ggtgcctcgtgccgcccgcctcggtcga                ccgcggggcgccgcccgggcccctgg
                                                                                
  
  Query   ME <---Intron---> LQATGPSTPSFIQDEEGNILDSR--GGGGDPVQFVFEDIHWTSEISREDAAR
          || <    82bp    > ||||||| |/|| |||||//|||  | ||||||||||/||| ||||||/|||
  Target  ME                LQATGPSVPTFIHDEEGNVIDSRVGGAGGDPVQFVFEEIHWKSEISREEAAR
          ag                ccgagctgcatacggggagagacgggggggcgctgtggactatgaacggggc
          t                ataccgcctccttaaaagattaggtggcggactatttaatagacatggaaccg
          g                aggttaagtcccttcggctgttcggcactgcccgtgtggacggggctcggttg
                                                                                
  
  Query   RLEVTFYPFKKVP <---Intron---> YLPFSEAFERAQAENKLVHSILLWGALDDQSCUGSG <---
          |/||||||||||  <   691bp    > ||||||||/||||| ||||||||||||||||||||| <   
  Target  RMEVTFYPFKKVE                YLPFSEAFDRAQAEKKLVHSILLWGALDDQSCUGSG     
          caggattctaagg                tccttggtgcgcggaacgctatctggcggctttgtg     
          gtatctactaata                atctcactagcacaaattactttggctaaacgggc      
          cggcgccccggga                cgtcggctcccgagggggcccaggtagccgccatg      
                                                                       *        
  
  Query   Intron---> RTLRETVLESSPVLALLNQSFISSWSLVKELEDMQANE <---Intron---> QDPVE
          211bp    > |||||||||||||||||||||||||||||||||/||/  <   913bp    > |/|  
  Target             RTLRETVLESSPVLALLNQSFISSWSLVKELEDLQADP                QNPTL
                     caccgagcgatcgcgccacataaattcgagcggccggc                cacac
                    ggctgacttagccttcttaagttgggcttaataatacac                aacct
                    gatcggacgatgccctgccgcccccgcggggagctgtc                catcca
                                                                                
  
  Query   SQRARLHLEKYSFPVEMMVALPNGTIVHH <---Intron---> INANYFLDETSMKPDEEP-TTFSFS
          |  | ||||||/||||||/||||||/||| <   156bp    > ||||||||/|/|||/|/  | ||||
  Target  SHLAALHLEKYNFPVEMMLALPNGTVVHH                INANYFLDQTAMKPEEDDGTMFSFS
          actggcccgatatcggaatgccagaggcc                aagattcgcagaacgggggaatatt
          gatcctataaaatctatttctcagcttaa                tacaattaacctacaaaagcttgtc
          ccgtagccgaccccgggggttccccgtcc                ccacccgcggcggcggtctagctca
                                                                                
  
  Query   GGFEDPSTATYISFLKEGLEKAKDYVGQ
          ||||||||/||| ||/|||||||/|/ |
  Target  GGFEDPSTSTYIRFLQEGLEKAKEYLEQ
          ggtggctatatactccggcgagagtcgc
          ggtaacccccatgttaagtaacaaataa
          gctgcccaccccccggagggacaacggg
                                      
  

SECIS prediction
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SECIS image
Predicted by: Infernal (score 34.01)
Covels score: 30.84
Free Energy of structure: -26.4
Target name: JARO01007492.1:subseq_15446,85386_
Positions on target: 44989-45063 Strand: -
Distance between candidate CDS and SECIS: 568
Distance between Sec-UGA and SECIS: 952
SECIS grade: A

  SEQ  ACUUUGGCGCUUUGUGAAGGCCUGUUCCGAAAGCCCCCCCACGGGUGGGGGCAGGCCCGAUGUCACUGCCGAGGA
  SS   .((((((((.....{[[{((((((((((...................))))))))))}]]}.....)))))))).