Total number of SECIS elements predicted: 2
Number of known selenoproteins predicted: 1
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Selenoprotein id: 1 Category: known selenoprotein
Protein prediction
Predicted by: exonerate Blastx evalue: 8e-05
Query protein: gi|146261167|ref|NP_001078947.1| selenoprotein I [Xenopus laevis]
Positions on query: 1-400 Query length: 401
Target name: JARO01001505.1:subseq_4646,100074_
Positions on target: 46095-46148,50003-50071,50438-50546,51169-51243,54945-55207,56197-56305,56745-56793,57263-57443,57939-58121,59002-59106 Strand: +
Query MAWEYVSPEQLAGFDKYK <---Intron---> YSAVDTNPLSLYVMHPFWNSIVR <---Intron--
/ /|||/ |||/|||||| < 3854bp > ||||||||||/||||||||//|/ < 366bp
Target LLYEYVTQEQLSGFDKYK YSAVDTNPLSVYVMHPFWNTVVK
cctgtgacgcctgtgata tagggaacctgtgaccttaagga
ttaaatcaaatcgtaaaa agctacactctattactgactta
cttatccagggttttgcg ctctcctggcatcgcgtgcttgg
Query -> FFPTWLAPNLITFSGFLLLVFTFFLMAFFDPGFYASA <---Intron---> PGQVHVPNWVWILA
> |||||||||||/||/ || | //| || |||| < 622bp > |/ ||| |||/ |
Target ILPTWLAPNLITFTGFMFLVLNFVMLALFDFDFYASV QGKTHVPGWVWVAA
atcatcgcacaatagtatcgcatgatgctgtgttgtg cgaacgcgtgtggg
ttccgtccattctcgttttttattttcttatatacc tagacatcggtgtcc
tacagctctccgctccgcccgctaggtcctccctca aataacgacgggtat
Query GLLNFTSYTLD <---Intron---> GVDGKQARRTNSSTPLGELFDHGLDSWACIFFVVTVYSIFGRG
|/ |||/|||| < 3701bp > |||||||||||||||||||||||||||||||||||||||||||
Target GVFNFTAYTLD GVDGKQARRTNSSTPLGELFDHGLDSWACIFFVVTVYSIFGRG
ggtatagtacg ggggacgccaataaccggctgcgcgatgtattggagttatgcg
gttatccacta gtagaacggcacgcctgattaagtaggcgtttttctacttggg
cccccggtagc tttgagtccccccgaagggtctcgccgcctcttccgcccccca
Query ETGVSVLVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDLSQVT <---Intron---> ISCVYLVTA
|||| | || /|| ||||||||||||||||/||||||||/|||| < 989bp > || ||//||
Target ETGVDVSTLYYILWSVLFSFILSHWEKYNTGVLFLPWGYDISQVT ISVVYIITA
gaggggtacttacttgctttactctgataaggctcctgtgaacga atggtaaag
acgtatcctaattgctttctttcagaaaacgttttcggaatgatc tcttattcc
gccctgcgccccggcgccccccccgggccctgtcgggcccccag cctttctagt
Query MVGVEAWYTPAFFNILYRDLFTSMIVGC <---Intron---> ALCVTLPMSLYNVFKAY <---Intr
/||||||| | /| |||||| ||/|| < 439bp > | |||||||||| ||| < 469b
Target VVGVEAWYKPILWNFHYRDLFTVMILGC AFTVTLPMSLYNVLKAY
ggggggttacactatctagctagaacgt gtagaccaactagtagt
ttgtacgaacttgataagattcttttg gctctctctgtaattac
cgtgaggtacaggcctcgcgctggtgc tgcagctcgtcctcggg
Query on---> RDNTLKHGSLYEAMLPIISPVLLFILTTFWIFASPSNILEQHPRLFYFMVGTTFANITCQL <
p > | /||||||||| || /||||||||// |/ |||/||// ||/|| |||| |||/||/| <
Target RRGSLKHGSLYEAFLPFLSPVLLFILSSLWVHLSPSDILQKQPRIFYLMVGTAFANVTCKL
cagtcacgtctggtcctctcgcctacttctgcctcagaccacccattcaggagtgagatac
agggctaagctaacttcttccttttttcctgtatccgattaaacgttatttgcctcatcga
ccaccgacccgcgccgccgcaggccctcgggtcccctttgggggcccccggcactctctcg
Query ---Intron---> IVCQMSNTRCQPLSWLLLPLTLAVGVSVSGVDSQYETLALVMVTVLVTVAHIHYGV
495bp > |||||||||||| ||||||/ | | / ||/ || | | /| /||/||||
Target IVCQMSNTRCQPFSWLLLPMALLVLLVSSGLVQHSETAVLYTWTAVVLLAHVHYGV
agtcaaaactcctatccccagccgccgatgcgccagaggctatagggccgcgctgg
tttgatgacggactggtttctcttttttgcgttaagaccttacgccttttcataagt
gcgcggccgctacccggtgcgtggggggcatgggctgaggcccgcaggggttcccgc
Query NVVNQ <---Intron---> LSKHFNILPFSLKKPNTDULEEEEKIGLQSAVV
/|| | < 880bp > || ||/| ||||/|//||| ||||/|||/| |
Target SVVQQ LSAHFSIFAFSLKRPSSDUL-EEEKVGLQAAEV
tggcc tagctaatgttcaacatgtc-gggaggccgggg
cttaa tgcatgttctctagcgcagt-aaaatgtaccat
tggga gctcccctccagggctacaa-gggactgggggg
*
SECIS prediction Predicted by: Infernal (score 31.63)
Covels score: 30.07
Free Energy of structure: -14.6
Target name: JARO01001505.1:subseq_4646,100074_
Positions on target: 60029-60102 Strand: +
Distance between candidate CDS and SECIS: 922
Distance between Sec-UGA and SECIS: 961
SECIS grade: A
SEQ UUAGUUUGAGCUUGAAUGAAGAGUAUAACUCAAACCCUAUUUUGGACCAGUUAUACUCUGAAGGAACAAGCUUU
SS ..((((((........([[(((((((((((..................)))))))))))]])....))))))..