TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000114_2.0 # Protein # Selenoprotein U2 (SelU2) # Human
(Homo sapiens)
         (226 letters)

Database: genome.fa 
           47,351 sequences; 2,188,353,730 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KN342762.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic...    83   2e-16

>KN342762.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic scaffold
             scaffold20684, whole genome shotgun sequence
          Length = 68143

 Score = 83.2 bits (204), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 38/52 (73%), Positives = 44/52 (84%)
 Frame = +1

Query: 142   QSPHIKSNLLSGSLQSLWRAVTGPLFDFQGDPAQQGGTLILGPGNNIHFIHR 193
             QSPH+KSNLLSGS++S+WRA+T P FD QGDPAQQGGTLILGPG    FI +
Sbjct: 19525 QSPHVKSNLLSGSIRSIWRAMTSPAFDLQGDPAQQGGTLILGPGKLTCFILK 19680



 Score = 71.2 bits (173), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 30/43 (69%), Positives = 37/43 (86%)
 Frame = +2

Query: 184   PGNNIHFIHRDRNRLDHKPINSVLQLVGVQHVNFTNRPSVIHV 226
             PG+ +HF+H D+NRLDH PIN+VLQL GV+ VNFTN+P VIHV
Sbjct: 20504 PGSEVHFLHFDKNRLDHAPINTVLQLAGVKTVNFTNKPQVIHV 20632



 Score = 57.4 bits (137), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 24/40 (60%), Positives = 31/40 (77%)
 Frame = +2

Query: 104   IEPFCKLTGYSHEIYVDPEREIYKRLGMKRGEEIASSGQS 143
             ++PFC LT Y HE+YVDP+  +YK LGMKRGE   SSG++
Sbjct: 18242 LQPFCNLTRYPHEMYVDPDLALYKILGMKRGEAATSSGRN 18361



 Score = 43.5 bits (101), Expect = 0.005,   Method: Compositional matrix adjust.
 Identities = 18/21 (85%), Positives = 19/21 (90%)
 Frame = +2

Query: 67    LCYICKEYVEDLAKIPRSFLQ 87
             LCY CKEYVEDLAKIP+ FLQ
Sbjct: 16469 LCYACKEYVEDLAKIPKDFLQ 16531



 Score = 33.9 bits (76), Expect = 5.0,   Method: Compositional matrix adjust.
 Identities = 15/18 (83%), Positives = 17/18 (94%)
 Frame = +1

Query: 88    EANVTLIVIGQSSYHHIE 105
             +ANV LIVIGQSSYHHI+
Sbjct: 17794 DANVRLIVIGQSSYHHIK 17847


  Database: genome.fa
    Posted date:  Sep 15, 2015 12:21 AM
  Number of letters in database: 2,188,353,730
  Number of sequences in database:  47,351
  
Lambda     K      H
   0.320    0.138    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 47351
Number of Hits to DB: 579,056,852
Number of extensions: 8715599
Number of successful extensions: 40434
Number of sequences better than 10.0: 3
Number of HSP's gapped: 40432
Number of HSP's successfully gapped: 7
Length of query: 226
Length of database: 729,451,243
Length adjustment: 125
Effective length of query: 101
Effective length of database: 723,532,368
Effective search space: 73076769168
Effective search space used: 73076769168
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)