TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00001058_2.0 # Protein # Selenoprotein S (SelS) # Chicken
(Gallus gallus)
         (194 letters)

Database: genome.fa 
           47,351 sequences; 2,188,353,730 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KN343631.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic...    57   1e-07
KN326473.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic...    33   5.9  

>KN343631.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic scaffold
              scaffold21586, whole genome shotgun sequence
          Length = 229554

 Score = 56.6 bits (135), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 28/52 (53%), Positives = 35/52 (67%), Gaps = 2/52 (3%)
 Frame = -2

Query: 29     TVGALLSSYGWYILLACVAIYLIVQKI--SPYLRMRPSSQQGATGAAVEPDM 78
              +VGALLS+YGWY+LLAC+AIYL+VQK+  S   R RP     A G    P +
Sbjct: 180350 SVGALLSAYGWYLLLACIAIYLLVQKVAESTRARSRPDPPGAAAGVCPPPRL 180195



 Score = 50.4 bits (119), Expect = 1e-05,   Method: Compositional matrix adjust.
 Identities = 23/29 (79%), Positives = 27/29 (93%)
 Frame = -1

Query: 73     AVEPDMVVRRQEALLASRLRMQEELNAQA 101
               +EPD+VVRRQEAL A+RL+MQEELNAQA
Sbjct: 177906 CLEPDVVVRRQEALAAARLKMQEELNAQA 177820



 Score = 43.5 bits (101), Expect = 0.003,   Method: Compositional matrix adjust.
 Identities = 19/21 (90%), Positives = 20/21 (95%)
 Frame = -1

Query: 120    IEMWESMQEGKSYKGNLKLSQ 140
              I MWESMQEGKSYKGNLKL+Q
Sbjct: 176424 IAMWESMQEGKSYKGNLKLNQ 176362



 Score = 37.4 bits (85), Expect = 0.31,   Method: Compositional matrix adjust.
 Identities = 15/15 (100%), Positives = 15/15 (100%)
 Frame = -1

Query: 167    GYNPLSGEGGGTCSW 181
              GYNPLSGEGGGTCSW
Sbjct: 174177 GYNPLSGEGGGTCSW 174133


>KN326473.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic scaffold
              scaffold3372, whole genome shotgun sequence
          Length = 235099

 Score = 33.5 bits (75), Expect = 5.9,   Method: Compositional matrix adjust.
 Identities = 21/44 (47%), Positives = 26/44 (59%), Gaps = 2/44 (4%)
 Frame = +3

Query: 26     LQHTVGALLSSYGWYILLACVAI--YLIVQKISPYLRMRPSSQQ 67
              L+H  G L SS+GWY+LL  V I  YLI   I P  +M  S Q+
Sbjct: 200739 LKHGSGQLGSSFGWYMLLRPVQIGNYLIQIWIQPIWKMADSIQR 200870


  Database: genome.fa
    Posted date:  Sep 15, 2015 12:21 AM
  Number of letters in database: 2,188,353,730
  Number of sequences in database:  47,351
  
Lambda     K      H
   0.312    0.129    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 47351
Number of Hits to DB: 370,542,759
Number of extensions: 4382398
Number of successful extensions: 23970
Number of sequences better than 10.0: 6
Number of HSP's gapped: 23964
Number of HSP's successfully gapped: 10
Length of query: 194
Length of database: 729,451,243
Length adjustment: 122
Effective length of query: 72
Effective length of database: 723,674,421
Effective search space: 52104558312
Effective search space used: 52104558312
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 49 (23.5 bits)