TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000070_2.0 # Protein # Selenoprotein H (SelH) # Human
(Homo sapiens)
         (122 letters)

Database: genome.fa 
           47,351 sequences; 2,188,353,730 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KN331620.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic...    64   3e-11

>KN331620.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic scaffold
             scaffold8777, whole genome shotgun sequence
          Length = 74145

 Score = 63.9 bits (154), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 29/46 (63%), Positives = 37/46 (80%)
 Frame = -1

Query: 45    RVYGRNAAALSQALRLEAPELPVKVNPTKPRRGSFEVTLLRPDGSS 90
             RVYGRNA+A+S+ALR     L V++NP KPRR SFEV+L++ DGSS
Sbjct: 17622 RVYGRNASAVSEALRHAVDNLTVEINPEKPRRNSFEVSLVKEDGSS 17485



 Score = 53.9 bits (128), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 31/62 (50%), Positives = 37/62 (59%), Gaps = 11/62 (17%)
 Frame = -2

Query: 72    TKPRRGSFEV-----------TLLRPDGSSAELWTGIKKGPPRKLKFPEPQEVVEELKKY 120
             TKPR G  +            +LL       ELW+GIKKGPPRKLKFPEP++VVE LK  
Sbjct: 17249 TKPRHGLLQYNPVTSTLSVTPSLLCLHILVVELWSGIKKGPPRKLKFPEPEKVVEALKSS 17070

Query: 121   LS 122
             L+
Sbjct: 17069 LA 17064


  Database: genome.fa
    Posted date:  Sep 15, 2015 12:21 AM
  Number of letters in database: 2,188,353,730
  Number of sequences in database:  47,351
  
Lambda     K      H
   0.314    0.133    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 47351
Number of Hits to DB: 268,919,281
Number of extensions: 3712870
Number of successful extensions: 10955
Number of sequences better than 10.0: 3
Number of HSP's gapped: 10954
Number of HSP's successfully gapped: 4
Length of query: 122
Length of database: 729,451,243
Length adjustment: 97
Effective length of query: 25
Effective length of database: 724,858,196
Effective search space: 18121454900
Effective search space used: 18121454900
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 47 (22.7 bits)