TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000067_2.0 # Protein # 15 kDa selenoprotein (Sel15) #
Human (Homo sapiens)
         (162 letters)

Database: genome.fa 
           47,351 sequences; 2,188,353,730 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KN331551.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic...    88   5e-19

>KN331551.1 Gavialis gangeticus isolate Ggan-Ray unplaced genomic scaffold
             scaffold8695, whole genome shotgun sequence
          Length = 135074

 Score = 88.2 bits (217), Expect = 5e-19,   Method: Compositional matrix adjust.
 Identities = 43/52 (82%), Positives = 46/52 (88%)
 Frame = -3

Query: 111   KLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI 162
             K+     ++YVRGSDPVLKLLDD GNIAEELSILKWNTDSVEEFLSEKLERI
Sbjct: 83298 KVIPFFSLQYVRGSDPVLKLLDDTGNIAEELSILKWNTDSVEEFLSEKLERI 83143



 Score = 67.8 bits (164), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 35/68 (51%), Positives = 42/68 (61%)
 Frame = -2

Query: 18     LLATVLQAVSAFGAEFSSEACRELGFXXXXXXXXXXXXGQFNLLQLDPDCRGCCQEEAQF 77
              L   +   +S +G + SSEACRELGF            GQF+L QLDP CR CCQEEAQF
Sbjct: 130942 LFKYIHFQLSVYGTKLSSEACRELGFSSNLLCSSCSLLGQFSLNQLDPFCRQCCQEEAQF 130763

Query: 78     ETKKLYAG 85
              ET+K+  G
Sbjct: 130762 ETRKVRFG 130739



 Score = 46.6 bits (109), Expect = 1e-04,   Method: Compositional matrix adjust.
 Identities = 22/28 (78%), Positives = 24/28 (85%)
 Frame = -2

Query: 76     QFETKKLYAGAILEVCGUKLGRFPQVQA 103
              QF T +LYAGA+LEVCG KLGRFPQVQ 
Sbjct: 105979 QFFTLQLYAGAVLEVCG*KLGRFPQVQG 105896



 Score = 43.5 bits (101), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 25/48 (52%), Positives = 28/48 (58%)
 Frame = -2

Query: 98    FPQVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILK 145
             FP   AFVRSDKPKLFRGLQIK      P +     N +  EE S+ K
Sbjct: 85801 FPLTLAFVRSDKPKLFRGLQIKVSNDFSPFIL----NSSC*EEFSVHK 85670


  Database: genome.fa
    Posted date:  Sep 15, 2015 12:21 AM
  Number of letters in database: 2,188,353,730
  Number of sequences in database:  47,351
  
Lambda     K      H
   0.323    0.140    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 47351
Number of Hits to DB: 391,503,309
Number of extensions: 4559227
Number of successful extensions: 14966
Number of sequences better than 10.0: 1
Number of HSP's gapped: 14965
Number of HSP's successfully gapped: 4
Length of query: 162
Length of database: 729,451,243
Length adjustment: 119
Effective length of query: 43
Effective length of database: 723,816,474
Effective search space: 31124108382
Effective search space used: 31124108382
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 48 (23.1 bits)