TBLASTN 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= SPP00000077_2.0 # Protein # Selenoprotein M (SelM) # Human
(Homo sapiens)
         (145 letters)

Database: genome.fa 
           133,062 sequences; 684,497,465 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BADN01035956.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    54   6e-08
BADN01035957.1 Thunnus orientalis DNA, contig: superscaffoldBa00...    49   1e-06

>BADN01035956.1 Thunnus orientalis DNA, contig: superscaffoldBa00000876_0013, whole
            genome shotgun sequence
          Length = 9132

 Score = 54.3 bits (129), Expect = 6e-08,   Method: Compositional matrix adjust.
 Identities = 24/42 (57%), Positives = 34/42 (80%)
 Frame = -1

Query: 93   ERIPLSEMTREEINALVQELGFYRKAAPDAQVPPEYVWAPAK 134
            +RI LS+MTR EIN L+ +LGFY+KA P+ +VP E+ ++PAK
Sbjct: 8811 QRIALSDMTRSEINELLGKLGFYKKAQPEDEVPEEFRFSPAK 8686


>BADN01035957.1 Thunnus orientalis DNA, contig: superscaffoldBa00000876_0014, whole
            genome shotgun sequence
          Length = 4147

 Score = 48.5 bits (114), Expect(2) = 1e-06,   Method: Compositional matrix adjust.
 Identities = 23/33 (69%), Positives = 26/33 (78%)
 Frame = -3

Query: 68   HNLVMKHLPGADPELVLLGRRYEELERIPLSEM 100
            HNLVMKH+PGADPELVLL   +EEL+  P S M
Sbjct: 1427 HNLVMKHIPGADPELVLLNHYFEELDVSPPSVM 1329



 Score = 23.5 bits (49), Expect(2) = 1e-06,   Method: Compositional matrix adjust.
 Identities = 11/20 (55%), Positives = 13/20 (65%)
 Frame = -2

Query: 55   EVKAFVTQDIPFYHNLVMKH 74
            +VKAFV QDIP  + L   H
Sbjct: 1578 QVKAFVVQDIPL*YPLTPVH 1519


  Database: genome.fa
    Posted date:  Oct 9, 2014  7:00 PM
  Number of letters in database: 684,497,465
  Number of sequences in database:  133,062
  
Lambda     K      H
   0.317    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 133062
Number of Hits to DB: 82,594,695
Number of extensions: 868418
Number of successful extensions: 3380
Number of sequences better than 1.0e-02: 2
Number of HSP's gapped: 3380
Number of HSP's successfully gapped: 3
Length of query: 145
Length of database: 228,165,821
Length adjustment: 109
Effective length of query: 36
Effective length of database: 213,662,063
Effective search space: 7691834268
Effective search space used: 7691834268
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 40 (20.0 bits)